Anti-TTF1 – Rabbit (WRAB-1231)使用说明
产品信息
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品牌
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Seven Hills Bioreagents
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货号
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WRAB-1231
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规格
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100 µL
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宿主
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兔
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反应种属
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人、鼠
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克隆类型
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多克隆
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偶联物
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无
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缓冲液
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0.01% sodium azide
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抗原
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重组鼠TTF-1融合6*his蛋白
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保存条件
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储存在-20℃，避免反复冻融
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蛋白背景
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甲状腺转录因子-1（TTF-1）是同源域转录因子家族的成员，并且在调节甲状腺，肺和脑内表达的基因中起作用。
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产品使用
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实验应用
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建议稀释倍数
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Western Blot
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1: 5000
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Immunohistochemistry
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1:1000 – 1:2000
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说明：最佳稀释倍数与浓度应由研究人员确认
应用实例
图片为成年小鼠肺组织，用6%正常山羊血清预包埋，并用抗大鼠TTF-1兔血清（1:2000稀释）染色。通过DAB（黑色）观察TTF-1，并用核快红（粉红色）复染。放大倍数10倍。
参考文献
1. Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93.
2. Stahlman MT, Gray ME, Whitsett JA. 'Expression of thyroid transcription factor-1(TTF-1) in fetal and neonatal human lung.' J Histochem Cytochem. 1996 Jul;44(7):673-8.
3. Khoor A, Whitsett JA, Stahlman MT, Olson SJ, Cagle PT. 'Utility of surfactant protein B precursor and thyroid transcription factor 1 in differentiating adenocarcinoma of the lung from malignant mesothelioma.' Hum Pathol. 1999 Jun;30(6):695-700.
4. deFelice M, Silberschmidt D, DiLauro R, Xu Y, Wert SE, Weaver TE, Bachurski CJ, Clark JC, Whitsett JA. 'TTF-1 phosphorylation is required for peripheral lung morphogenesis, perinatal survival, and tissue-specific gene expression.' J Biol Chem. 2003 Sep 12;278(37):35574-83.
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