标签归档：vegf

重组恒河猴VEGF R2/KDR蛋白(His标签) Rhesus macaque VEGF R2/KDR Protein

发表于由

重组恒河猴VEGF R2/KDR蛋白(His标签) Rhesus macaque VEGF R2/KDR Protein

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名（Synonyms）

VEGFR; KDR;CD309; VEGFR-21

表达区间及表达系统（Source）

Rhesus macaque VEGF R2/KDR Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala20-Glu764.[Accession | F7E313]

分子量大小（Molecular Weight）

The protein has a predicted MW of 84.37 kDa. Due to glycosylation, the protein migrates to 115-145 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Rhesus macaque VEGF R2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 19.6ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名（Synonyms）

VEGFR; KDR;CD309; VEGFR-21

表达区间及表达系统（Source）

Rhesus macaque VEGF R2/KDR Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala20-Glu764.[Accession | F7E313]

分子量大小（Molecular Weight）

The protein has a predicted MW of 84.37 kDa. Due to glycosylation, the protein migrates to 115-145 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized Rhesus macaque VEGF R2, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 19.6ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组人VEGF-C His标签VEGF-C|Recombinant Human VEGF-C

发表于由

重组人VEGF-C His标签VEGF-C|Recombinant Human VEGF-C

产品说明书

FAQ

COA

已发表文献

 

Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a subfamily of the angiogenic VEGF angiogenic factors. VEGF-C is synthesized as a 58 kDa molecule that consists of a VEGF homolgy domain (VHD) flanked by N- and C-terminal propeptides. The proprotein undergoes covalent homodimerization and stepwise proteolytic processing to generate ligands with increasing affinity for VEGF R3/Flt-4. Fully processed VEGF-C containing just the 21 kDa VHD can additionally bind and activate VEGF R2/KDR/Flk-1. Fully processed human VEGF-C shares 98% amino acid sequence identity with mouse and rat VEGF-C. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. VEGF-C and VEGF R3 are usually co-expressed at sites with lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

 

Product Properties

Synonyms

Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM

Accession

P49767

Source

HEK293 Cells-derived human VEGF-C, Thr103-Arg227 with a C-terminal polyhistidine tag.

Molecular Weight

The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.

Tag

His

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE.

Biological Activity

1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at2μg/mL(100 μL/well) can bind VEGFR3 hFc, the EC50 of VEGFR3 hFc is 2-15 ng/mL.
2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.
3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Normally 5% – 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

  1. Avoid repeated freeze-thaw cycles.
  2. For your safety and health, please wear lab coats and disposable gloves for operation.
  3. For research use only.

HB221025

重组人VEGF-C His标签VEGF-C|Recombinant Human VEGF-C

暂无内容

重组人VEGF-C His标签VEGF-C|Recombinant Human VEGF-C

暂无内容

 

Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a subfamily of the angiogenic VEGF angiogenic factors. VEGF-C is synthesized as a 58 kDa molecule that consists of a VEGF homolgy domain (VHD) flanked by N- and C-terminal propeptides. The proprotein undergoes covalent homodimerization and stepwise proteolytic processing to generate ligands with increasing affinity for VEGF R3/Flt-4. Fully processed VEGF-C containing just the 21 kDa VHD can additionally bind and activate VEGF R2/KDR/Flk-1. Fully processed human VEGF-C shares 98% amino acid sequence identity with mouse and rat VEGF-C. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. VEGF-C and VEGF R3 are usually co-expressed at sites with lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

 

Product Properties

Synonyms

Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM

Accession

P49767

Source

HEK293 Cells-derived human VEGF-C, Thr103-Arg227 with a C-terminal polyhistidine tag.

Molecular Weight

The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.

Tag

His

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE.

Biological Activity

1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at2μg/mL(100 μL/well) can bind VEGFR3 hFc, the EC50 of VEGFR3 hFc is 2-15 ng/mL.
2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.
3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Normally 5% – 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

  1. Avoid repeated freeze-thaw cycles.
  2. For your safety and health, please wear lab coats and disposable gloves for operation.
  3. For research use only.

HB221025

重组人VEGF-C His标签VEGF-C|Recombinant Human VEGF-C

暂无内容

重组人VEGF-C His标签VEGF-C|Recombinant Human VEGF-C

暂无内容

重组FITC标记人VEGF165蛋白 Recombinant FITC-Labeled Human VEGF165 Protein,His-Avi Tag

发表于由

重组FITC标记人VEGF165蛋白 Recombinant FITC-Labeled Human VEGF165 Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名（Synonyms）

VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165

表达区间及表达系统（Source）

FITC-Labeled Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala27-Arg191.[Accession | P15692-4]

分子量大小（Molecular Weight）

The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-33 kDa under reduced (R) condition, 45-55 kDa under Non reducing (N) condition based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized FITC-Labeled Human VEGF165, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 22.3ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名（Synonyms）

VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165

表达区间及表达系统（Source）

FITC-Labeled Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala27-Arg191.[Accession | P15692-4]

分子量大小（Molecular Weight）

The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-33 kDa under reduced (R) condition, 45-55 kDa under Non reducing (N) condition based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized FITC-Labeled Human VEGF165, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 22.3ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

小鼠VEGF ELISA试剂盒 Mouse VEGF ELISA Kit

发表于由

小鼠VEGF ELISA试剂盒 Mouse VEGF ELISA Kit

产品说明书

FAQ

COA

已发表文献

 

血管内皮生长因子（VEGF）是特异性最高的血管生成调控因子，可为内皮细胞的迁移和肿瘤细胞的转移提供形态学基础。作为肿瘤基质和毛细血管网形成的基础，VEGF可抑制肿瘤细胞的凋亡，机制尚不明确。

翌圣Mouse VEGF ELISA(酶联免疫吸附测定)试剂盒是一种体外酶联免疫吸附测定试剂盒，用于定量测定小鼠血清、血浆和细胞培养上清中的血管内皮生长因子(Mouse VEGF)。特异性抗血管内皮生长因子抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本，经过孵育，样本中存在的血管内皮生长因子与固相抗体结合。洗涤去除未结合的物质后，加入检测抗体（生物素标记）孵育结合，经洗涤，再加入酶结合物(HRP标记链霉亲和素)孵育结合。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中血管内皮生长因子的浓度成正比。加入终止液终止反应，在450 nm 主波长，630nm次波长测定吸光度值。

 

产品信息

货号

98020ES48 / 98020ES96 

规格

48 T / 96 T

检测范围

6.25-400 pg/mL

检测方法

双抗夹心法

检测物种

小鼠

检测时长

4小时30分钟

灵敏度

0.11 pg/mL

稀释线性

96.7-126.9%

回收率

77.1 – 124%

板内差

4.4%

板间差

6.33%

 

实验数据

  1. 标曲数据

数据拟合绘制标准曲线，生成的标曲用于实验数据分析。标准曲线图

  1. 灵敏度检测

血管内皮生长因子的最低检测限为0.11 pg/mL，通过使用重复检测20次零孔OD值的均值与标准差计算得出。

  1. 精密度检测

酶标板内精密度

3个已知浓度的样本酶标板内重复测定20次，评估酶标板内的精密度。

酶标板间精密度

3个已知浓度的样本酶标板间重复检测36次，评估酶标板间的精密度。

项目

板内精密度

板间精密度

样本

1

2

3

1

2

3

20

20

20

36

36

36

平均值

9.35

41.89

177.21

9.89

42.80

179.60

标准差

0.36

1.98

8.26

0.81

2.70

8.00

变异系数（%）

3.84

4.73

4.66

8.21

6.32

4.45
  1. 回收率检测

  通过不同水平的血管内皮生长因子添加于样本中来测定回收率，其回收率如下:

样本类型

平均回收率(%)

范围（%）

血清

88.8

77.1-103.6

血浆

89.5

86.4-91.5

细胞培养上清

110.0

97.1-124.0
  1. 稀释线性检测

血清稀释比例

平均期望值(%)

范围（%）

1:02

107.6

105.3-109.5

1:04

112.1

109.9-115.2

1:08

116.3

110.0-124.2

1:16

122.9

112.2-126.9

 

血浆稀释比例

平均期望值(%)

范围（%）

1:02

103.7

102.4-105

1:04

107.6

104.1-111.1

1:08

111.8

111.3-112.4

1:16

122.4

118.5-126.3

 

细胞培养上清稀释比例

平均期望值(%)

范围（%）

1:02

102.8

100.3-105.3

1:04

104.1

104.0-104.2

1:08

99.4

96.7-102.1

1:16

99.1

98.1-100.1
  1. 样本值

应用本试剂盒，检测若干样本，背景不详。

样本类型

样本数目

平均值（pg/mL）

样本值（pg/mL）

细胞培养上清

10

n.d.

n.d.

血清

10

85.20

58-101

血浆

5

116.80

98-134

n.d. 指样本浓度值低于检测范围内的6.25 pg/mL  

  1. 测定特异性

本试剂盒识别天然和重组小鼠血管内皮生长因子。配制50ng/ml的下述因子进行了特异性的评估，没有观察到明显的交叉反应和干扰影响。

Recombinant mouse:

Recombinant human:

Other Recombinants:

VEGF R2/Fc Chimera

VEGF121

canine VEGF

 

VEGF165

feline VEGF

 

VEGF165/PIGF

 

 

 

 

 

 

 

 

 

 

 

 

 

检测简图

 

小鼠VEGF ELISA试剂盒 Mouse VEGF ELISA Kit

 

疑问解答

 

问题

造成原因

解决方法

标曲不好

移液量不准确

检查移液器，按时校准，仔细操作，充分混匀时盖紧管口并尽量避免泡沫。

稀释方法不恰当

显色值偏低

孵育时间太短

给予足够的孵育时间，样本和溶解的标准品隔夜后更换。

移液量不足或稀释不当

校准移液器和规范操作

CV偏高

酶标板清洗不当

使用正确的洗涤工序；如果使用洗板机，检查所有的端口是否堵塞。

受污染的洗液

准备新鲜洗液

灵敏度较低

试剂盒储存不当

按照产品组分表进行保存。

 

小鼠VEGF ELISA试剂盒 Mouse VEGF ELISA Kit

暂无内容

小鼠VEGF ELISA试剂盒 Mouse VEGF ELISA Kit

暂无内容

 

血管内皮生长因子（VEGF）是特异性最高的血管生成调控因子，可为内皮细胞的迁移和肿瘤细胞的转移提供形态学基础。作为肿瘤基质和毛细血管网形成的基础，VEGF可抑制肿瘤细胞的凋亡，机制尚不明确。

翌圣Mouse VEGF ELISA(酶联免疫吸附测定)试剂盒是一种体外酶联免疫吸附测定试剂盒，用于定量测定小鼠血清、血浆和细胞培养上清中的血管内皮生长因子(Mouse VEGF)。特异性抗血管内皮生长因子抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本，经过孵育，样本中存在的血管内皮生长因子与固相抗体结合。洗涤去除未结合的物质后，加入检测抗体（生物素标记）孵育结合，经洗涤，再加入酶结合物(HRP标记链霉亲和素)孵育结合。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中血管内皮生长因子的浓度成正比。加入终止液终止反应，在450 nm 主波长，630nm次波长测定吸光度值。

 

产品信息

货号

98020ES48 / 98020ES96 

规格

48 T / 96 T

检测范围

6.25-400 pg/mL

检测方法

双抗夹心法

检测物种

小鼠

检测时长

4小时30分钟

灵敏度

0.11 pg/mL

稀释线性

96.7-126.9%

回收率

77.1 – 124%

板内差

4.4%

板间差

6.33%

 

实验数据

  1. 标曲数据

数据拟合绘制标准曲线，生成的标曲用于实验数据分析。标准曲线图

  1. 灵敏度检测

血管内皮生长因子的最低检测限为0.11 pg/mL，通过使用重复检测20次零孔OD值的均值与标准差计算得出。

  1. 精密度检测

酶标板内精密度

3个已知浓度的样本酶标板内重复测定20次，评估酶标板内的精密度。

酶标板间精密度

3个已知浓度的样本酶标板间重复检测36次，评估酶标板间的精密度。

项目

板内精密度

板间精密度

样本

1

2

3

1

2

3

20

20

20

36

36

36

平均值

9.35

41.89

177.21

9.89

42.80

179.60

标准差

0.36

1.98

8.26

0.81

2.70

8.00

变异系数（%）

3.84

4.73

4.66

8.21

6.32

4.45
  1. 回收率检测

  通过不同水平的血管内皮生长因子添加于样本中来测定回收率，其回收率如下:

样本类型

平均回收率(%)

范围（%）

血清

88.8

77.1-103.6

血浆

89.5

86.4-91.5

细胞培养上清

110.0

97.1-124.0
  1. 稀释线性检测

血清稀释比例

平均期望值(%)

范围（%）

1:02

107.6

105.3-109.5

1:04

112.1

109.9-115.2

1:08

116.3

110.0-124.2

1:16

122.9

112.2-126.9

 

血浆稀释比例

平均期望值(%)

范围（%）

1:02

103.7

102.4-105

1:04

107.6

104.1-111.1

1:08

111.8

111.3-112.4

1:16

122.4

118.5-126.3

 

细胞培养上清稀释比例

平均期望值(%)

范围（%）

1:02

102.8

100.3-105.3

1:04

104.1

104.0-104.2

1:08

99.4

96.7-102.1

1:16

99.1

98.1-100.1
  1. 样本值

应用本试剂盒，检测若干样本，背景不详。

样本类型

样本数目

平均值（pg/mL）

样本值（pg/mL）

细胞培养上清

10

n.d.

n.d.

血清

10

85.20

58-101

血浆

5

116.80

98-134

n.d. 指样本浓度值低于检测范围内的6.25 pg/mL  

  1. 测定特异性

本试剂盒识别天然和重组小鼠血管内皮生长因子。配制50ng/ml的下述因子进行了特异性的评估，没有观察到明显的交叉反应和干扰影响。

Recombinant mouse:

Recombinant human:

Other Recombinants:

VEGF R2/Fc Chimera

VEGF121

canine VEGF

 

VEGF165

feline VEGF

 

VEGF165/PIGF

 

 

 

 

 

 

 

 

 

 

 

 

 

检测简图

 

小鼠VEGF ELISA试剂盒 Mouse VEGF ELISA Kit

 

疑问解答

 

问题

造成原因

解决方法

标曲不好

移液量不准确

检查移液器，按时校准，仔细操作，充分混匀时盖紧管口并尽量避免泡沫。

稀释方法不恰当

显色值偏低

孵育时间太短

给予足够的孵育时间，样本和溶解的标准品隔夜后更换。

移液量不足或稀释不当

校准移液器和规范操作

CV偏高

酶标板清洗不当

使用正确的洗涤工序；如果使用洗板机，检查所有的端口是否堵塞。

受污染的洗液

准备新鲜洗液

灵敏度较低

试剂盒储存不当

按照产品组分表进行保存。

 

小鼠VEGF ELISA试剂盒 Mouse VEGF ELISA Kit

暂无内容

小鼠VEGF ELISA试剂盒 Mouse VEGF ELISA Kit

暂无内容

Human VEGF121 重组人血管内皮生长因子121(人VEGF121)

发表于由

Human VEGF121 重组人血管内皮生长因子121(人VEGF121)

产品说明书

FAQ

COA

已发表文献

Vascular endothelial growth factor 121 (VEGF121) is a protein that plays a crucial role in the growth and survival of blood vessels. It stimulates the formation of new blood vessels, a process known as angiogenesis, which is essential for normal development and tissue repair. VEGF121 has therapeutic applications for conditions such as ischemic heart disease and cancer.

 

性能参数

Synonyms

VEGFA; vascular permeability factor; vasculotropin;V EGF-A

Uniprot No.

P15692-9

Source

Recombinant Human VEGF121 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Ala27-Arg147.

Molecular Weight

The protein has a predicted MW of 16 kDa. And it migrates as 17-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. 

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge tubes before opening. Dissolve lyophilized protein with sterile  PBS to ensure the concentration is greater than 100 ug/mL.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

产品数据

Human VEGF121 重组人血管内皮生长因子121(人VEGF121)

Ver. CN20230609

 

Human VEGF121 重组人血管内皮生长因子121(人VEGF121)

暂无内容

Human VEGF121 重组人血管内皮生长因子121(人VEGF121)

暂无内容

Vascular endothelial growth factor 121 (VEGF121) is a protein that plays a crucial role in the growth and survival of blood vessels. It stimulates the formation of new blood vessels, a process known as angiogenesis, which is essential for normal development and tissue repair. VEGF121 has therapeutic applications for conditions such as ischemic heart disease and cancer.

 

性能参数

Synonyms

VEGFA; vascular permeability factor; vasculotropin;V EGF-A

Uniprot No.

P15692-9

Source

Recombinant Human VEGF121 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Ala27-Arg147.

Molecular Weight

The protein has a predicted MW of 16 kDa. And it migrates as 17-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. 

Purity

> 95% as determined by SDS-PAGE.

Endotoxin

<0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge tubes before opening. Dissolve lyophilized protein with sterile  PBS to ensure the concentration is greater than 100 ug/mL.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

产品数据

Human VEGF121 重组人血管内皮生长因子121(人VEGF121)

Ver. CN20230609

 

Human VEGF121 重组人血管内皮生长因子121(人VEGF121)

暂无内容

Human VEGF121 重组人血管内皮生长因子121(人VEGF121)

暂无内容