重组人TIGIT蛋白(hFc标签) Human TIGIT Proteing
产品说明书
FAQ
COA
已发表文献
性能参数
分子别名(Synonyms) |
VSTM3; VSIG9;TIGIT; |
表达区间及表达系统(Source) |
Human TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Met22-Pro141.[Accession | Q495A1-1] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on SDS-PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by SDS-PAGE and HPLC. |
活性(Activity) |
ELISA Data: Immobilized Human TIGIT, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA .Immobilized Biotinylated Human Nectin-2, His Tag at 1μg/ml (100μl/Well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.22μg/ml determined by ELISA. |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution) |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
性能参数
分子别名(Synonyms) |
VSTM3; VSIG9;TIGIT; |
表达区间及表达系统(Source) |
Human TIGIT Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Met22-Pro141.[Accession | Q495A1-1] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 48-50 kDa based on SDS-PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by SDS-PAGE and HPLC. |
活性(Activity) |
ELISA Data: Immobilized Human TIGIT, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 20.9ng/ml determined by ELISA .Immobilized Biotinylated Human Nectin-2, His Tag at 1μg/ml (100μl/Well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 0.22μg/ml determined by ELISA. |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution) |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.