StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated)
一键复制产品信息 产品编号: AF2927
产品包装:50μl
选择包装
50μl
数
量
StrepII Tag Mouse mAb (HRP Conjugated)(StrepII Tag 小鼠单抗)(AF2927)
回复
一键复制产品信息 说明书下载
价格: ¥ 388.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2927 | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl | 388.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IHC, ICC | StrepII-tagged protein in all species | – | – |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | – | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) |
| Category | Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | – |
| Cellular location | – |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic StrepII tag peptide coupled to KLH |
| Sequence | WSHPQFEK |
| Gene ID | – |
| SwissProt | – |
| Synonyms | – |
| Category | Polypeptide protein tag |
| Background | Streptavidin is a tetrameric protein expressed in Streptomyces avidinii. Because of its high affinity for the vitamin h-biotin, Streptavidin is commonly used in the fields of molecular biology and biotechnology. The Strep-tag was originally selected from a genetic library to specifically bind to a proteolytically truncated “core” version of streptavidin. Over the years, the Strep -tag was systemically optimized, to permit a greater flexibility in the choice of attachment site. Further, its interaction partner, Streptavidin was also optimized to increase peptide-binding capacity, which resulted in the development of Strep-Tactin. The so called Strep-tag system, consisting of Strep-tag and Strep-Tactin, has proven particularly useful for the functional isolation and analysis of protein complexes in proteome research. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2927 | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2852 | Flag Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2855 | Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2858 | HA Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2861 | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2864 | Myc Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2867 | Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2870 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2873 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2876 | His Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2879 | His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2882 | GFP Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2885 | GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2888 | GST Tag Mouse Monoclonal Antibody | 50μl/200μl |
| AF2891 | GST Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
| AF2894 | V5 Tag Mouse Monoclonal Antibody | 50μl/200μl |
| AF2897 | V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
| AF2900 | Glu-Glu Tag Mouse Monoclonal Antibody | 50μl |
| AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50μl |
| AF2909 | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2912 | MBP Tag Mouse Monoclonal Antibody | 50μl |
| AF2915 | MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2918 | PA Tag Mouse Monoclonal Antibody | 50μl |
| AF2921 | PA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50μl |
| AF2927 | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2930 | S Tag Mouse Monoclonal Antibody | 50μl |
| AF2933 | S Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2936 | TEV-His Tag Mouse Monoclonal Antibody | 50μl |
| AF2939 | TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2942 | Target Tag Mouse Monoclonal Antibody | 50μl |
| AF2945 | Target Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2948 | TRX Tag Mouse Monoclonal Antibody | 50μl |
| AF2951 | TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2954 | AVI Tag Mouse Monoclonal Antibody | 50μl |
| AF2957 | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
标签抗体
HRP标记一抗
使用本产品的文献：
StrepII Tag Mouse mAb(StrepII Tag 小鼠单抗)(AF2924)
StrepII Tag Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF2924
产品包装:50μl
选择包装
50μl
数
量
说明书下载
价格: ¥ 299.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50μl | 299.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IP, IF, IHC, ICC | StrepII-tagged protein in all species | – | – |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | 1:50 | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | StrepII Tag Mouse Monoclonal Antibody |
| Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | – |
| Cellular location | – |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic StrepII tag peptide coupled to KLH |
| Sequence | WSHPQFEK |
| Gene ID | – |
| SwissProt | – |
| Synonyms | – |
| Category | Polypeptide protein tag |
| Background | Streptavidin is a tetrameric protein expressed in Streptomyces avidinii. Because of its high affinity for the vitamin h-biotin, Streptavidin is commonly used in the fields of molecular biology and biotechnology. The Strep-tag was originally selected from a genetic library to specifically bind to a proteolytically truncated “core” version of streptavidin. Over the years, the Strep -tag was systemically optimized, to permit a greater flexibility in the choice of attachment site. Further, its interaction partner, Streptavidin was also optimized to increase peptide-binding capacity, which resulted in the development of Strep-Tactin. The so called Strep-tag system, consisting of Strep-tag and Strep-Tactin, has proven particularly useful for the functional isolation and analysis of protein complexes in proteome research. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
StrepII Tag Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2852 | Flag Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2855 | Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2858 | HA Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2861 | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2864 | Myc Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2867 | Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2870 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2873 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2876 | His Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2879 | His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2882 | GFP Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2885 | GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2888 | GST Tag Mouse Monoclonal Antibody | 50μl/200μl |
| AF2891 | GST Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
| AF2894 | V5 Tag Mouse Monoclonal Antibody | 50μl/200μl |
| AF2897 | V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
| AF2900 | Glu-Glu Tag Mouse Monoclonal Antibody | 50μl |
| AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50μl |
| AF2909 | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2912 | MBP Tag Mouse Monoclonal Antibody | 50μl |
| AF2915 | MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2918 | PA Tag Mouse Monoclonal Antibody | 50μl |
| AF2921 | PA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50μl |
| AF2927 | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2930 | S Tag Mouse Monoclonal Antibody | 50μl |
| AF2933 | S Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2936 | TEV-His Tag Mouse Monoclonal Antibody | 50μl |
| AF2939 | TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2942 | Target Tag Mouse Monoclonal Antibody | 50μl |
| AF2945 | Target Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2948 | TRX Tag Mouse Monoclonal Antibody | 50μl |
| AF2951 | TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
| AF2954 | AVI Tag Mouse Monoclonal Antibody | 50μl |
| AF2957 | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
标签抗体
使用本产品的文献：