标签归档：rnai

RNAi-Ready pSIREN-RetroQ载体说明书

发表于由

 RNAi载体，RNAi-Ready pSIREN-RetroQ载体，Clontech

RNAi-Ready pSIREN-RetroQ

型号 载体名称 出品公司 载体用途
VRC0345 RNAi-Ready pSIREN-Retro Q Clontech RNAi载体
载体描述: 

RNAi-Ready pSIREN-RetroQ is a self-inactivating retroviral expression vector designed to express a small hairpin RNA （shRNA） using the human U6 promoter （PU6; RNA Pol III-dependent）. RNAi-Ready pSIREN-RetroQ is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an oligonuceotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQ construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA. The vector contains a puromycin resistance gene for the selection of stable transfectants.

质粒图谱: 

RNAi-Ready pSIREN-Retro Q-ZsGreen

发表于由

 RNAi载体,RNAi-Ready pSIREN-Retro Q-ZsGreen载体

RNAi-Ready pSIREN-Retro Q-ZsGreen

型号 载体名称 出品公司 载体用途
VRC0347 RNAi-Ready pSIREN-Retro Q-ZsGreen Clontech RNAi载体
产品参数: 

Promoter: Human U6
Reporter: ZsGreen
Selection: prokaryotic—ampicillin
Replication: prokaryotic—Col E1 ori （low copy）, viral—SV40 ori

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

RNAi-Ready pSIREN-RetroQ-ZsGreen derives from our original gene silencing retroviral vector RNAi-Ready pSIREN-RetroQ. pSIREN-RetroQ-ZsGreen is a self-inactivating retroviral expression vector designed to express a small hairpin RNA （shRNA） using the human U6 promoter. This vector is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an annealed DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. The pSIREN-RetroQ-ZsGreen construct can be transfected as a plasmid expression vector. For generation of active retrovirus for infecting hard-to-transfect cell lines, transfect the pSIREN-RetroQ-ZsGreen construct into a retroviral packaging cell line and collect the viral supernatant.

质粒图谱: 

pSilencer5.1-U6 Retro RNAi载体说明书

发表于由

 Ambion RNAi载体，RNAi载体，pSilencer5.1-U6 Retro载体

pSilencer5.1-U6 Retro

型号 载体名称 出品公司 载体用途
VRA0580 pSilencer5.1-U6 Retro Ambion RNAi载体
载体描述: 

pSilencer 5.1 Retro vectors harness retroviruses’ ability to stably integrate into the host genome of nearly all mitotically active cells and include a puromycin resistance gene for selection of cells that express the recombinant DNA. The pSilencer 5.1 Retro vectors enable researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. This technology expands the capabilities of RNA interference research to address long-term alteration of gene expression.
pSilencer 5.1-U6 Retro uses the U6 promoter.

质粒图谱: 

RNAi-Ready pSIREN-Retro Q-DsRed-Express

发表于由

RNAi载体，RNAi-Ready pSIREN-Retro Q-DsRed-Express载体 

RNAi-Ready pSIREN-Retro Q-DsRed-Express

型号 载体名称 出品公司 载体用途
VRC0346 RNAi-Ready pSIREN-Retro Q-DsRed-Express Clontech RNAi载体
载体描述: 

RNAi-Ready pSIREN-RetroQ-DsRed-Express is a self-inactivating retroviral expression vector designed to express a small hairpin RNA （shRNA） using the human U6 promoter （PU6; RNA Pol III-dependent）. RNAi-Ready pSIREN-RetroQ-DsRed-Express is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when a ds DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQDsRed-Express construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA.

质粒图谱: