重组PE标记人CD7蛋白 Recombinant PE-Labeled Human CD7 Protein,His-Avi Tag

发表于2024年12月23日由上海金畔生物科技有限公司

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	CD7 molecule; CD7; LEU-9; Tp40; TP41; GP40
表达区间及表达系统（Source）	PE-Labeled Human CD7 Protein is expressed from HEK293 His tag and Avi tag at the C-Terminus. It contains Ala26-Pro180.[Accession \| P09564]
分子量大小（Molecular Weight）	The protein has a predicted MW of 19.3 kDa.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）
制剂（Formulation）	Supplied as 0.22μm filtered solution in PBS (pH 7.4).

储存条件

The product should be stored at -85~-65℃ for 6 months from date of receipt.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	CD7 molecule; CD7; LEU-9; Tp40; TP41; GP40
表达区间及表达系统（Source）	PE-Labeled Human CD7 Protein is expressed from HEK293 His tag and Avi tag at the C-Terminus. It contains Ala26-Pro180.[Accession \| P09564]
分子量大小（Molecular Weight）	The protein has a predicted MW of 19.3 kDa.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）
制剂（Formulation）	Supplied as 0.22μm filtered solution in PBS (pH 7.4).

储存条件

The product should be stored at -85~-65℃ for 6 months from date of receipt.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组PE标记人CD7蛋白 Recombinant PE-Labeled Human CD7 Protein,His-Avi Tag

暂无内容

Recombinant CPB, Expressed in E.coli 大肠杆菌表达重组羧肽酶B

发表于2024年12月22日由上海金畔生物科技有限公司

Recombinant CPB, Expressed in E.coli 大肠杆菌表达重组羧肽酶B

产品说明书

FAQ

COA

已发表文献

产品简介

羧肽酶B又称肽酰-L-赖氨酸（L-赖氨酸）水解酶、精蛋白酶，专一水解蛋白质C-末端碱性氨基酸（赖氨酸(Lysine、K)、精氨酸(Arginine、R)、组氨酸(Histidine、H)）的氨基端；分子量为33.8 kD，等电点为6.0，最适pH为7.5-9.0。羧肽酶B的活性受精氨酸、赖氨酸的竞争性抑制，金属离子螯合剂如EDTA等抑制酶活。

翌圣重组羧肽酶B采用大肠杆菌表达，在GMP法规下生产，不含任何动物源成分，无动物源性的病毒污染，氨基酸序列与大鼠胰腺羧肽酶B完全一致，具有与动物源性羧肽酶B相同的酶学性质，可替代动物源性羧肽酶 B用于各种生物技术过程中。

产品应用

1. 重组胰岛素及其类似物的生产。

2. 蛋白质C-末端氨基酸的测定。

3. 蛋白质C-末端组氨酸标签的去除。

4. 其他重组多肽类物质的生产。

5. 某些特殊化合物的酶促合成。

产品信息

货号	20417JP03 / 20417JP10
规格	1 mg / 10 mg

产品性质

来源（Source）	大肠杆菌表达
分子量（Molecular Weight）	理论值33.8±3.4 kDa
外观（Appearance）	白色、类白色、类黄色粉末
酶浓度（Enzyme Concentration）	≥170 USP units/mg pro
活性定义（Activity Definition）	25℃，pH7.6，1 min催化1 μmoL马脲酰-L-精氨酸水解的酶量为一个酶活单位
质量保证（Quality assurance）	SDS-PAGE胶检测仅可见清晰单一的目的条带；不含其他蛋白酶，无非特异性切割

储存条件

冻干粉2~8℃保存，有效期2年。

用无菌水或者25 mM Tris-HCl （pH 7.6）溶解后存于-25~-15℃，反复冻融10次，无活性损失。

使用方法（应用举例）

用无菌水或者25mM Tris-HCl（pH 7.6）溶解重组羧肽酶B，使酶液浓度为1-10 mg/mL；

酶量：目的蛋白（质量比）=1:50-1:1000，最适pH为7.5-9.0。

注意事项

1. 本产品仅作科研用途。

2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

Ver.CN20240219

产品简介

产品应用

1. 重组胰岛素及其类似物的生产。

2. 蛋白质C-末端氨基酸的测定。

3. 蛋白质C-末端组氨酸标签的去除。

4. 其他重组多肽类物质的生产。

5. 某些特殊化合物的酶促合成。

产品信息

货号	20417JP03 / 20417JP10
规格	1 mg / 10 mg

产品性质

来源（Source）	大肠杆菌表达
分子量（Molecular Weight）	理论值33.8±3.4 kDa
外观（Appearance）	白色、类白色、类黄色粉末
酶浓度（Enzyme Concentration）	≥170 USP units/mg pro
活性定义（Activity Definition）	25℃，pH7.6，1 min催化1 μmoL马脲酰-L-精氨酸水解的酶量为一个酶活单位
质量保证（Quality assurance）	SDS-PAGE胶检测仅可见清晰单一的目的条带；不含其他蛋白酶，无非特异性切割

储存条件

冻干粉2~8℃保存，有效期2年。

用无菌水或者25 mM Tris-HCl （pH 7.6）溶解后存于-25~-15℃，反复冻融10次，无活性损失。

使用方法（应用举例）

用无菌水或者25mM Tris-HCl（pH 7.6）溶解重组羧肽酶B，使酶液浓度为1-10 mg/mL；

酶量：目的蛋白（质量比）=1:50-1:1000，最适pH为7.5-9.0。

注意事项

1. 本产品仅作科研用途。

2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

Ver.CN20240219

Recombinant CPB, Expressed in E.coli 大肠杆菌表达重组羧肽酶B

暂无内容

重组人VEGF-C His标签VEGF-C|Recombinant Human VEGF-C

发表于2024年12月19日由上海金畔生物科技有限公司

重组人VEGF-C His标签VEGF-C|Recombinant Human VEGF-C

产品说明书

FAQ

COA

已发表文献

Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a subfamily of the angiogenic VEGF angiogenic factors. VEGF-C is synthesized as a 58 kDa molecule that consists of a VEGF homolgy domain (VHD) flanked by N- and C-terminal propeptides. The proprotein undergoes covalent homodimerization and stepwise proteolytic processing to generate ligands with increasing affinity for VEGF R3/Flt-4. Fully processed VEGF-C containing just the 21 kDa VHD can additionally bind and activate VEGF R2/KDR/Flk-1. Fully processed human VEGF-C shares 98% amino acid sequence identity with mouse and rat VEGF-C. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. VEGF-C and VEGF R3 are usually co-expressed at sites with lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, resulting in enhanced lymph flow and metastasis to regional lymph nodes.

Product Properties

Synonyms	Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM
Accession	P49767
Source	HEK293 Cells-derived human VEGF-C, Thr103-Arg227 with a C-terminal polyhistidine tag.
Molecular Weight	The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.
Tag	His
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95% by SDS-PAGE.
Biological Activity	1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at2μg/mL(100 μL/well) can bind VEGFR3 hFc, the EC50 of VEGFR3 hFc is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED₅₀for this effect is typically 0.1-0.5 μg/mL.
Endotoxin	< 1.0 EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Normally 5% – 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only.

HB221025

Product Properties

Synonyms	Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM
Accession	P49767
Source	HEK293 Cells-derived human VEGF-C, Thr103-Arg227 with a C-terminal polyhistidine tag.
Molecular Weight	The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation.
Tag	His
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Purity	> 95% by SDS-PAGE.
Biological Activity	1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at2μg/mL(100 μL/well) can bind VEGFR3 hFc, the EC50 of VEGFR3 hFc is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED₅₀for this effect is typically 0.1-0.5 μg/mL.
Endotoxin	< 1.0 EU per 1μg of the protein by the LAL method.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Normally 5% – 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

Avoid repeated freeze-thaw cycles.
For your safety and health, please wear lab coats and disposable gloves for operation.
For research use only.

HB221025

重组人VEGF-C His标签VEGF-C|Recombinant Human VEGF-C

暂无内容

Recombinant Ten-Eleven Translocase (TET)

发表于2024年12月12日由上海金畔生物科技有限公司

Recombinant Ten-Eleven Translocase (TET)

产品说明书

FAQ

COA

已发表文献

Recombinant Ten-Eleven Translocase (TET)是基于TET酶改造和重组表达的高效 Fe²⁺和α-酮戊二酸（α-KG）依赖的双加氧酶，通过一系列氧化反应，可将5-甲基胞嘧啶（5mC）转化为5-羟甲基胞嘧啶（5hmC），再转化为5-甲酰胞嘧啶（5fC）和5-羧基胞嘧啶（5caC）。再通过转化剂或酶法的作用，5caC进一步被转化成尿嘧啶(U)，通过PCR将尿嘧啶（U）转化成胸腺嘧啶（T），从而达到单碱基分辨率和高准确性的DNA甲基化位点鉴定。

组分信息

组分名称	14554ES80	14554ES90
Recombinant Ten-Eleven Translocase (TET) (5 μg/μL)	200 μL	1000 μL

储存条件

-25~-15℃保存，有效期1年。

使用说明

EM-seq投入量~40ug/T*，该酶的反应温度为37℃，充分反应一般需60 min，需进行终止反应。

*：酶投入量可根据实验需求进行适当调整。

注意事项

1. 该酶解冻后应置于冰上操作。

2. 该酶适用于酶法甲基化测序（EM-seq），DNA模板投入量为1-200 ng。

3. 本产品仅作科研用途。

4. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

Ver.CN20231023

组分信息

组分名称	14554ES80	14554ES90
Recombinant Ten-Eleven Translocase (TET) (5 μg/μL)	200 μL	1000 μL

储存条件

-25~-15℃保存，有效期1年。

使用说明

EM-seq投入量~40ug/T*，该酶的反应温度为37℃，充分反应一般需60 min，需进行终止反应。

*：酶投入量可根据实验需求进行适当调整。

注意事项

1. 该酶解冻后应置于冰上操作。

2. 该酶适用于酶法甲基化测序（EM-seq），DNA模板投入量为1-200 ng。

3. 本产品仅作科研用途。

4. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

Ver.CN20231023

Recombinant Ten-Eleven Translocase (TET)

暂无内容

Recombinant Human Ubiquitin Activating Enzyme E1 (UBE1)

发表于2024年12月11日由上海金畔生物科技有限公司

Recombinant Human Ubiquitin Activating Enzyme E1 (UBE1)

产品说明书

FAQ

COA

已发表文献

This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions.

产品性质

别名(Synonyms)	UBA1, UBA6
来源(Source)	E.coli
分子量 (Molecular Weight)	Approximately 118 kDa.
氨基酸序列 (AA Sequence)	MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDT SNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIY DDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
外观 (Physical Appearance)	Liquid
标签(Tag)	None
纯度(Purity)	> 95% by SDS-PAG
缓冲液(Buffer)	50mM PBS, pH7.0, 150mM NaCl, 1mM DTT.

储存条件

-25~-15℃保存

注意事项

1. 避免反复冻融。

2. 本产品仅作科研用途。

2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

反应缓冲液配方

名称	组分	浓度
10 × Reaction Buffer（200 ul）	Tris PH8.0	500 mM
	DTT	6 mM
	ddH2O	to 200μL
10 x ATP-Mg （200 ul）	ATP	100 mM
	MgCl2	100 mM
	ddH2O	to 200μL

参考阳性反应体系

组分	体积 (μL)
ddH2O	to 20 μL
10 × Reaction Buffer	2 μL
10 × E1 Enzyme	2 μL
5 × Ubiquitin	4 μL
10 × ATP-Mg	2 μL
20 × E2 Enzyme	1 μL
10 × E3 Ligase Itch (Positive control)	2 μL
20 × S5a substrate (Positive control)	1 μL

Ver.CN20231024

产品性质

别名(Synonyms)	UBA1, UBA6
来源(Source)	E.coli
分子量 (Molecular Weight)	Approximately 118 kDa.
氨基酸序列 (AA Sequence)	MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDT SNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIY DDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
外观 (Physical Appearance)	Liquid
标签(Tag)	None
纯度(Purity)	> 95% by SDS-PAG
缓冲液(Buffer)	50mM PBS, pH7.0, 150mM NaCl, 1mM DTT.

储存条件

-25~-15℃保存

注意事项

1. 避免反复冻融。

2. 本产品仅作科研用途。

2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。

反应缓冲液配方

名称	组分	浓度
10 × Reaction Buffer（200 ul）	Tris PH8.0	500 mM
	DTT	6 mM
	ddH2O	to 200μL
10 x ATP-Mg （200 ul）	ATP	100 mM
	MgCl2	100 mM
	ddH2O	to 200μL

参考阳性反应体系

组分	体积 (μL)
ddH2O	to 20 μL
10 × Reaction Buffer	2 μL
10 × E1 Enzyme	2 μL
5 × Ubiquitin	4 μL
10 × ATP-Mg	2 μL
20 × E2 Enzyme	1 μL
10 × E3 Ligase Itch (Positive control)	2 μL
20 × S5a substrate (Positive control)	1 μL

Ver.CN20231024

Recombinant Human Ubiquitin Activating Enzyme E1 (UBE1)

暂无内容

重组人CEACAM-3蛋白 Recombinant Human CEACAM-3 Protein,hFc Tag

发表于2024年12月9日由上海金畔生物科技有限公司

重组人CEACAM-3蛋白 Recombinant Human CEACAM-3 Protein,hFc Tag

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	CGM1;CD66d;CEACAM3
表达区间及表达系统（Source）	Human CEACAM-3 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Lys35-Gly155.[Accession \| P40198-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Human CEACAM-7, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	CGM1;CD66d;CEACAM3
表达区间及表达系统（Source）	Human CEACAM-3 Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Lys35-Gly155.[Accession \| P40198-1]
分子量大小（Molecular Weight）	The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Human CEACAM-7, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组人SMPD1蛋白 Recombinant Human SMPD1 Protein,His Tag

发表于2024年12月2日由上海金畔生物科技有限公司

重组人SMPD1蛋白 Recombinant Human SMPD1 Protein,His Tag

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	ASM;
表达区间及表达系统（Source）	Human SMPD1 Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus. It contains Met1-Pro628.[Accession \| NP_000534.3]
分子量大小（Molecular Weight）	The protein has a predicted MW of 66.53 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
制剂（Formulation）	Supplied as 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 25% glycerol (pH 7.5).

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	ASM;
表达区间及表达系统（Source）	Human SMPD1 Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus. It contains Met1-Pro628.[Accession \| NP_000534.3]
分子量大小（Molecular Weight）	The protein has a predicted MW of 66.53 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
制剂（Formulation）	Supplied as 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 25% glycerol (pH 7.5).

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组人SMPD1蛋白 Recombinant Human SMPD1 Protein,His Tag

暂无内容

重组猴痘病毒E8L蛋白Recombinant Monkeypox Virus E8L Protein,His Tag

发表于2024年11月29日由上海金畔生物科技有限公司

重组猴痘病毒E8L蛋白Recombinant Monkeypox Virus E8L Protein,His Tag

产品说明书

FAQ

COA

已发表文献

猴痘病毒（Monkeypox Virus, MPXV）属于正痘病毒属（Orthopoxvirus genus）。一般来说，同属病毒具有相似的生物学特性。猴痘病毒可能与“痘病毒属模式病毒”—牛痘传播机制类似。

猴痘病毒E8L蛋白是病毒入侵宿主细胞重要基因，在病毒入侵宿主完成复制的过程中作为MV的细胞表面结合蛋白与细胞表面的硫酸软骨素结合，以提供病毒粒子附着在靶细胞上。有研究表明利用siRNA技术能有效拮抗Monkeypox virus的A6R和E8L基因，由此构建siA6-a和si8 -d能破坏MPV复制。

产品性质

别名	E8L
Accession	QJQ40248.1
表达系统	E.coli.
表达区间	Met1-Thr275.
标签	N-His
分子量	The protein migrates to 50-60 kDa based on SDS-PAGE result.
纯度	> 95% as determined by SDS-PAGE.
内毒素	< 1 EU per 1μg of the protein by the LAL method.
制剂	蛋白溶于PBS, 200 mM L-精氨酸(pH 7.4)中，经0.22 μm滤膜过滤后冻干。
复溶	用无菌蒸馏水复溶至0.1 mg/mL（通常使用1 mg/mL溶液进行冻干）。

运输与保存方法

冰袋运输。

冻干粉-20 ℃保存一年有效期。

复溶后，蛋白溶液–20 °C保存3个月，2-8°C 保存一周。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

HB221027

产品性质

别名	E8L
Accession	QJQ40248.1
表达系统	E.coli.
表达区间	Met1-Thr275.
标签	N-His
分子量	The protein migrates to 50-60 kDa based on SDS-PAGE result.
纯度	> 95% as determined by SDS-PAGE.
内毒素	< 1 EU per 1μg of the protein by the LAL method.
制剂	蛋白溶于PBS, 200 mM L-精氨酸(pH 7.4)中，经0.22 μm滤膜过滤后冻干。
复溶	用无菌蒸馏水复溶至0.1 mg/mL（通常使用1 mg/mL溶液进行冻干）。

运输与保存方法

冰袋运输。

冻干粉-20 ℃保存一年有效期。

复溶后，蛋白溶液–20 °C保存3个月，2-8°C 保存一周。

建议第一次使用时分装冻存，避免反复冻融。

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

HB221027

重组猴痘病毒E8L蛋白Recombinant Monkeypox Virus E8L Protein,His Tag

暂无内容

重组食蟹猴CD46蛋白 Recombinant Cynomolgus CD46 Protein,His Tag

发表于2024年11月28日由上海金畔生物科技有限公司

重组食蟹猴CD46蛋白 Recombinant Cynomolgus CD46 Protein,His Tag

产品说明书

FAQ

COA

已发表文献

性能参数

分子别名（Synonyms）	TLX; CD46; MCP; MIC10; AHUS2; TRA2.10
表达区间及表达系统（Source）	Cynomolgus CD46 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys35-Asp329.[Accession \| A0A2K5WCS2]
分子量大小（Molecular Weight）	The protein has a predicted MW of 34.05 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Cynomolgus CD46, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 0.67μg/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

性能参数

分子别名（Synonyms）	TLX; CD46; MCP; MIC10; AHUS2; TRA2.10
表达区间及表达系统（Source）	Cynomolgus CD46 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys35-Asp329.[Accession \| A0A2K5WCS2]
分子量大小（Molecular Weight）	The protein has a predicted MW of 34.05 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.
内毒素（Endotoxin）	Less than 1EU per μg by the LAL method.
纯度（Purity）	> 95% as determined by SDS-PAGE and HPLC.
活性（Activity）	ELISA Data: Immobilized Cynomolgus CD46, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD46 Antibody, hFc Tag with the EC50 of 0.67μg/ml determined by ELISA.
制剂（Formulation）	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
重构方法（Reconstitution）	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

Recombinant SARS-COV-2 Nucleocapsid Protein,His-Avi Tag

发表于2024年11月28日由上海金畔生物科技有限公司

Recombinant SARS-COV-2 Nucleocapsid Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

Nucleocapsid protein (N) is the major viral structural component; its main function is to protect and encapsidate the viral RNA forming viral RNP complex. It is encoded by the S segment vRNA and is abundantly expressed in the cytoplasm of infected cells.

产品信息

别名	Nucleocapsid; NP; NC
Accession	QHD43423.2
表达区间及表达系统	Recombinant SARS-COV-2 Nucleocapsid Protein is expressed from E.coli with His tag and Avi tag at the N-terminal. It contains Ser2-Ala419.
分子量	Approximately 48.9 kDa. Due to glycosylation, the protein migrates to 47–52 kDa based on Tris-Bis PAGE result.
纯度	> 95% as determined by Tris-Bis PAGE.
内毒素	Less than 1EU per μg by the LAL method.
制剂	Supplied as 0.22μm filtered solution in 10mM PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

储存条件

The product should be stored at -25~-15℃ for 1 year.

1 month, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves，for your safety。

2.This product is for research use only.

产品数据

Recombinant SARS-COV-2 Nucleocapsid Protein,His-Avi Tag

产品信息

别名	Nucleocapsid; NP; NC
Accession	QHD43423.2
表达区间及表达系统	Recombinant SARS-COV-2 Nucleocapsid Protein is expressed from E.coli with His tag and Avi tag at the N-terminal. It contains Ser2-Ala419.
分子量	Approximately 48.9 kDa. Due to glycosylation, the protein migrates to 47–52 kDa based on Tris-Bis PAGE result.
纯度	> 95% as determined by Tris-Bis PAGE.
内毒素	Less than 1EU per μg by the LAL method.
制剂	Supplied as 0.22μm filtered solution in 10mM PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.

储存条件

The product should be stored at -25~-15℃ for 1 year.

1 month, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~-15℃ °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

注意事项

1.Please operate with lab coats and disposable gloves，for your safety。

2.This product is for research use only.

产品数据

Recombinant SARS-COV-2 Nucleocapsid Protein,His-Avi Tag

暂无内容