标签归档：rat

Bio-Serv动物模型饲料MD300-2 Rat MD’s, Baytril (2 mg/Tablet)Bacon Flavor, 100/Bottle

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Bio-Serv动物模型饲料MD300-2 Rat MD’s, Baytril (2 mg/Tablet)Bacon Flavor, 100/Bottle

MD300-2

F05266

F07813

Product Details

5 gm Tablet – 1″ dia. x 1/4″ high
Shelf Life: 6 months, cool dry storage

Baytril is an effective antibiotic drug used in a variety of species including rodents. Baytril MD’s are highly palatable and encourage appetite post-operatively promoting a more rapid recovery versus other conventional methods. They are an ideal pain-free alternative to stressful and injurious post-operative injections. Baytril MD’s can be customized to include anti-inflammatory drugs such as Rimadyl and Meloxicam to provide both medications easily and affordably.

  • Highly palatable, nutritionally complete, grain-based, bacon flavored tablets
  • Easy to administer: Place on cage floor for easy access
  • Each 5 gram bacon-flavored tablet contains: 2 mg Baytril
  • Rat Average Daily Dose: One 5 gram Tablet/Rat per day provides daily medication needs and is a dietary supplement to standard feed
  • Introduction of Bio-Serv’s Placebo or MD tablet 1-2 days prior to surgery will decrease novelty and increase acceptance
  • More convenient and less risk of dehydration compared to using medicated water
  • Safe alternative to traditional dosing methods
  • Cold tableting process eliminates drug degradation
  • Can be customized to meet specific dosage requirements
  • Nutritionally Assayed

Gamma irradiation is available upon request. Please contact Customer Service for pricing and lead time.

Nutritional Profile
Protein
Fat
Fiber		 21.3
4.3
4.0		 %
%
%		 Ash
Moisture
Carbohydrate		 7.8
<10
54.0		 %
%
%
Calories: 3.39 kcal/gm

MD300-2 Rat MD  MD300-2 Nutritional Profile
  F05266 Nutritional Profile
        F07813 Nutritional Profile

Ingredients
Ground Corn, Banana Flakes, Dehulled Soybean Meal, Corn Gluten Meal, Ground Wheat, Corn Gluten Feed, Fish Meal, Dehydrated Alfalfa Meal, Casein, Dried Whey, Sucrose, Fructose, Dextrose, Dried Beet Pulp, Soybean Oil, Porcine Animal Fat (Preserved with BHA), Dried Brewers Yeast, Mineral Mix, Vitamin Mix, Magnesium Stearate, Calcium Silicate, Bacon Flavor, DL-Methionine, Choline Chloride, Sodium Propionate, Ascorbic Acid, Kaolin

Feeding Guidelines

For best acceptance, introduce Bio-Serv’s placebo or MD Tablets 1-2 days prior to surgery. Feed one MD tablet per rat per day to provide the daily medication dose and nutritional supplement. Place tablets at cage floor level for ease of access by the animal. Standard feed should remain in feed hopper.

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Bio-Serv动物模型饲料K3325 Rat Tunnel, Red, Certified

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上海金畔生物科技有限公司代理Bio-Serv模型动物饲料全线产品，欢迎访问官网了解更多产品信息。
Bio-Serv动物模型饲料K3325 Rat Tunnel, Red, Certified

K3325

K3326

K3339

Product Details
  • Certified (Contaminant Screened)
  • Made of high temp polycarbonate
  • Offers rodents shelter and security
  • Transparency makes routine cage checks
    easy and fast
  • Helps reduce boredom and aggressive behaivor
  • To increase physical activity and usable floor space,
    suspend with the optional stainless steel hanger
  • Autoclavable
  • Cagewasher safe
Dimensions
Tunnels – 6″ long x 3″ inside diameter; 1/8″ wall
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Rat BD-3 重组大鼠β防御素3(大鼠β-防御素蛋白3)

发表于由

Rat BD-3 重组大鼠β防御素3(大鼠β-防御素蛋白3)

产品说明书

FAQ

COA

已发表文献

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.

 

Product Properties

Synonyms

BD-3 Protein, DEFB-3 Protein, DEFB103 Protein, DEFB3 Protein, BD-3 Protein, BD3 Protein, BP-3 Protein, BP3 Protein

Accession

Q32Z14

GeneID

641623

Source

E.coli-derived rat Beta-defensin 3 protein,Lys23-Lys63

Molecular Weight

Approximately 4.5 kDa.

AA Sequence

KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20 μg/ml.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

 

HB220419

Rat BD-3 重组大鼠β防御素3(大鼠β-防御素蛋白3)

暂无内容

Rat BD-3 重组大鼠β防御素3(大鼠β-防御素蛋白3)

暂无内容

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.

 

Product Properties

Synonyms

BD-3 Protein, DEFB-3 Protein, DEFB103 Protein, DEFB3 Protein, BD-3 Protein, BD3 Protein, BP-3 Protein, BP3 Protein

Accession

Q32Z14

GeneID

641623

Source

E.coli-derived rat Beta-defensin 3 protein,Lys23-Lys63

Molecular Weight

Approximately 4.5 kDa.

AA Sequence

KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20 μg/ml.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

 

HB220419

Rat BD-3 重组大鼠β防御素3(大鼠β-防御素蛋白3)

暂无内容

Rat BD-3 重组大鼠β防御素3(大鼠β-防御素蛋白3)

暂无内容

Bio-Serv动物模型饲料9004 Rat Pellet Tube

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上海金畔生物科技有限公司代理Bio-Serv模型动物饲料全线产品，欢迎访问官网了解更多产品信息。
Bio-Serv动物模型饲料9004 Rat Pellet Tube

9004

9005

90045

90055

Product Details
Tubes:

  • Made of borosilicate glass
  • Easy, accurate and convenient method
    of measuring food intake
  • Narrow opening allows access to pellet
    without removal from the tube
  • Mouse tubes hold eight 1 gm pellets
  • Rat tubes hold eleven 1 gm pellets
  • Autoclavable
  • Cagewasher safe

Holders:

  • Made of stainless steel
  • Bottom tray catches any reclaimable pellet fines
  • Holder is designed to hang over lip of cage
  • Autoclavable
  • Cagewasher safe
Dimensions
Mouse Feeding Tube – 3 1/2″ tall x 1/2″ od
Mouse Tube Holder – 4 1/2″ tall x 1 3/8 " wide

Rat Feeding Tube – 5 1/4″ tall x 1/2″ od
Rat Tube Holder – 5 3/4″ tall x 1 3/8 " wide

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Rat BD-4 重组大鼠β防御素4(大鼠β-防御素蛋白4)

发表于由

Rat BD-4 重组大鼠β防御素4(大鼠β-防御素蛋白4)

产品说明书

FAQ

COA

已发表文献

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.  BD-4 is expressed in the testes, stomach, uterus, neutrophils, thyroid, lungs and kidneys. 

 

Product Properties

Synonyms

Defensin beta4，Beta-defensin 4，BD-4，Defensin, beta 4

Accession

O88514

GeneID

64389

Source

E.coli-derived rat Beta-defensin 4 protein, Gln23-Lys63

Molecular Weight

Approximately 4.4 kDa.

AA Sequence

QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 10 mM PB, pH 7.4, 500 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

 

HB220419

 

Rat BD-4 重组大鼠β防御素4(大鼠β-防御素蛋白4)

暂无内容

Rat BD-4 重组大鼠β防御素4(大鼠β-防御素蛋白4)

暂无内容

Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The α-defensins are distinguished from the β-defensins by the pairing of their three disulfide bonds. To date, six human β-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. β-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The β-defensin proteins are expressed as the C-terminal portion of precursors, and are released by proteolytic cleavage of a signal sequence and, in some cases, a propeptide sequence. β-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.  BD-4 is expressed in the testes, stomach, uterus, neutrophils, thyroid, lungs and kidneys. 

 

Product Properties

Synonyms

Defensin beta4，Beta-defensin 4，BD-4，Defensin, beta 4

Accession

O88514

GeneID

64389

Source

E.coli-derived rat Beta-defensin 4 protein, Gln23-Lys63

Molecular Weight

Approximately 4.4 kDa.

AA Sequence

QSINNPITCL TKGGVCWGPC TGGFRQIGTC GLPRVRCCKK K

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 0.1-100.0 ng/ml.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 10 mM PB, pH 7.4, 500 mM NaCl.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

 

HB220419

 

Rat BD-4 重组大鼠β防御素4(大鼠β-防御素蛋白4)

暂无内容

Rat BD-4 重组大鼠β防御素4(大鼠β-防御素蛋白4)

暂无内容

Rat GMF-β 重组大鼠胶质细胞成熟因子β(大鼠GMFβ)

发表于由

Rat GMF-β 重组大鼠胶质细胞成熟因子β(大鼠GMFβ)

产品说明书

FAQ

COA

已发表文献

The glia maturation factor beta belongs to the actin-binding proteins ADF family, GMF subfamily. It contains an ADF-H domain, but the research of crystallography and NMR reveals that there are structures different between human and mouse ADF-H domain. GMF-β is involved in the differentiation, maintenance, and regeneration of the nervous system. It also inhibition of proliferation of tumor cells.

 

Product Properties

Synonyms

Glia maturation factor beta, GMF-beta

Accession

Q63228

GeneID

81661

Source

E.coli-derived rat Glia Maturation Factor beta protein，Ser2-His142

Molecular Weight

Approximately 16.6 kDa.

AA Sequence

SESLVVCDVAEDLVEKLRKFRFRKETHNAAIIMKIDKDKRLVVLDEELEGVSPDELKDELPERQPRFIVYSYKYQHDDGRVSYPLCFIFSSPLGCKPEQQMMYAGSKNKLVQTAELTKVFEIRNTEDLTEWLREKLGFFH

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Biological Activity

Data is not available.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

 

HB220419

Rat GMF-β 重组大鼠胶质细胞成熟因子β(大鼠GMFβ)

暂无内容

Rat GMF-β 重组大鼠胶质细胞成熟因子β(大鼠GMFβ)

暂无内容

The glia maturation factor beta belongs to the actin-binding proteins ADF family, GMF subfamily. It contains an ADF-H domain, but the research of crystallography and NMR reveals that there are structures different between human and mouse ADF-H domain. GMF-β is involved in the differentiation, maintenance, and regeneration of the nervous system. It also inhibition of proliferation of tumor cells.

 

Product Properties

Synonyms

Glia maturation factor beta, GMF-beta

Accession

Q63228

GeneID

81661

Source

E.coli-derived rat Glia Maturation Factor beta protein，Ser2-His142

Molecular Weight

Approximately 16.6 kDa.

AA Sequence

SESLVVCDVAEDLVEKLRKFRFRKETHNAAIIMKIDKDKRLVVLDEELEGVSPDELKDELPERQPRFIVYSYKYQHDDGRVSYPLCFIFSSPLGCKPEQQMMYAGSKNKLVQTAELTKVFEIRNTEDLTEWLREKLGFFH

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Biological Activity

Data is not available.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

 

HB220419

Rat GMF-β 重组大鼠胶质细胞成熟因子β(大鼠GMFβ)

暂无内容

Rat GMF-β 重组大鼠胶质细胞成熟因子β(大鼠GMFβ)

暂无内容

Rat BD-1 重组大鼠β防御素1(大鼠β-防御素蛋白1)

发表于由

Rat BD-1 重组大鼠β防御素1(大鼠β-防御素蛋白1)

产品说明书

FAQ

COA

已发表文献

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.

 

Product Properties

Synonyms

BD1 Protein, DEFB-1 Protein, DEFB101 Protein, BD1 Protein

Accession

O89117

GeneID

83687

Source

E.coli-derived rat Beta-defensin 1 protein, Asp33-Ser69

Molecular Weight

Approximately 4.1 kDa.

AA Sequence

DQYRCLQNGG FCLRSSCPSH TKLQGTCKPD KPNCCRS

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

 

HB220419

Rat BD-1 重组大鼠β防御素1(大鼠β-防御素蛋白1)

暂无内容

Rat BD-1 重组大鼠β防御素1(大鼠β-防御素蛋白1)

暂无内容

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6 kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.

 

Product Properties

Synonyms

BD1 Protein, DEFB-1 Protein, DEFB101 Protein, BD1 Protein

Accession

O89117

GeneID

83687

Source

E.coli-derived rat Beta-defensin 1 protein, Asp33-Ser69

Molecular Weight

Approximately 4.1 kDa.

AA Sequence

DQYRCLQNGG FCLRSSCPSH TKLQGTCKPD KPNCCRS

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

 

HB220419

Rat BD-1 重组大鼠β防御素1(大鼠β-防御素蛋白1)

暂无内容

Rat BD-1 重组大鼠β防御素1(大鼠β-防御素蛋白1)

暂无内容

Rat IL-4 ELISA Kit

发表于由

Rat IL-4 ELISA Kit

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白细胞介素-4（IL-4），也称为B细胞刺激因子-1，是一种多效性细胞因子，诱导初始辅助性T细胞向Th2细胞分化，由肥大细胞、活化的T细胞和骨髓基质细胞产生。成熟的人IL-4与牛、小鼠和大鼠IL-4分别具有55%、39%和43%的氨基酸序列同一性。它是一种由T细胞产生的强效细胞因子，在较小程度上由肿瘤相关的自然杀伤细胞、嗜碱性粒细胞和肥大细胞产生。IL-4是分泌Th3的转化生长因子β的分化因子。IL-4通过信号转导子和转录激活子Stat6和一种名为4PS的170K蛋白的酪氨酸磷酸化激活两种不同的信号通路。

翌圣Rat IL-4 ELISA(酶联免疫吸附测定)试剂盒是一种体外酶联免疫吸附测定试剂盒，用于定量测定血清、血浆中的大鼠白介素4(Rat IL-4)。特异性抗大鼠白介素4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本，经过孵育，样本中存在的大鼠白介素4与固相抗体结合。洗涤去除未结合的物质后，加入检测抗体孵育结合，经洗涤，再加入酶结合物(Streptavidin-HRP)孵育结合。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中大鼠白介素4的浓度成正比。加入终止液终止反应，在450 nm 波长(参考波长570 – 630 nm)测定吸光度值。

 

产品信息

货号

98503ES48 / 98503ES96 

规格

48 T / 96 T

检测范围

15.63-1000 pg/mL

检测方法

双抗夹心法

检测物种

大鼠

检测时长

4.5小时

灵敏度

11.6 pg/mL

稀释线性

85 – 117%

回收率

76 – 123%

板内差

2.0%

板间差

3.0%

 

实验数据

  1. 标曲数据

数据拟合绘制标准曲线，生成的标曲用于实验数据分析。

标准曲线图

浓度（pg/mL）

显色值

平均值

校准值

1000

2.821

2.716

2.768

2.777

500

2.148

2.029

2.088

2.056

250

1.322

1.245

1.283

1.326

125

0.802

0.775

0.788

0.767

62.5

0.432

0.414

0.423

0.423

31.25

0.252

0.243

0.247

0.238

15.625

0.145

0.142

0.144

0.147

0

0.060

0.058

0.059

/

Rat IL-4 ELISA Kit

  1. 灵敏度检测

IL-4的最低检测限为11.6 pg/mL，通过使用重复检测20次零孔OD值的均值与标准差计算得出。

  1. 精密度检测

酶标板内精密度

3个已知浓度的样本酶标板内重复测定6次，评估酶标板内的精密度。

酶标板间精密度

3个已知浓度的样本酶标板间重复检测22次，评估酶标板间的精密度。

项目

板内精密度

板间精密度

样本

1

2

3

1

2

3

6

6

6

22

22

22

平均值

468.10

122.28

32.29

468.08

123.19

32.10

标准差

12.47

2.48

0.43

15.75

2.68

1.07

变异系数（%）

2.7

2.0

1.3

3.4

2.2

3.3
  1. 回收率检测

 通过不同水平的IL-4添加于样本中来测定回收率，其回收率如下:

样本类型

平均回收率(%)

范围（%）

血清

82.4

75.7-89.5

血浆

118.4

112.0-123.2

细胞培养上清

89.2

82.4-93.6
  1. 稀释线性检测

血清稀释比例

平均期望值(%)

范围（%）

1:02

99.0

94.8-105.1

1:04

96.8

95.3-99.6

1:08

104.7

103.3-105.8

1:16

110.0

105.7-116.7

 

血浆稀释比例

平均期望值(%)

范围（%）

1:02

98.7

95.1-104.8

1:04

100.1

94.5-107.5

1:08

103.6

97.5-106.9

1:16

92.4

85.2-104.5

 

细胞培养上清稀释比例

平均期望值(%)

范围（%）

1:02

100.0

97.4-101.9

1:04

101.3

96.8-104.4

1:08

107.2

100.9-112.9

1:16

104.7

94.8-112.7
  1. 样本值

应用本试剂盒，检测若干健康志愿者的样本，志愿者的用药史不详。

样本类型

样本数目

平均值（pg/mL）

样本值（pg/mL）

血清

10

n.d.

n.d.

血浆

8

n.d.

n.d.

细胞培养上清

4

n.d.

n.d.

n.d. 指样本浓度值低于检测范围内得15.63 pg/mL

  1. 测定特异性

本试剂盒识别天然和重组大鼠白介素4。下述因子进行了特异性的评估，没有观察到明显的交叉反应和干扰影响。

Recombinant Rat:

Recombinant Human:

Recombinant Mouse:

IL-1α

IL-10

IL-4

IL-4

IL-1β

IL-18

 

 

IL-2

IFN-γ

 

 

IL-6

TNF-α

 

 

检测简图

Rat IL-4 ELISA Kit

 

疑问解答

问题

造成原因

解决方法

标曲不好

移液量不准确

检查移液器，按时校准，仔细操作，充分混匀时盖紧管口并尽量避免泡沫。

稀释方法不恰当

显色值偏低

孵育时间太短

给予足够的孵育时间，样本和溶解的标准品隔夜后更换。

移液量不足或稀释不当

校准移液器和规范操作

CV偏高

酶标板清洗不当

使用正确的洗涤工序；如果使用洗板机，检查所有的端口是否堵塞。

受污染的洗液

准备新鲜洗液

灵敏度较低

试剂盒储存不当

按照产品组分表进行保存。

 

Ver.CN20231106

 

Rat IL-4 ELISA Kit

暂无内容

Rat IL-4 ELISA Kit

暂无内容

 

白细胞介素-4（IL-4），也称为B细胞刺激因子-1，是一种多效性细胞因子，诱导初始辅助性T细胞向Th2细胞分化，由肥大细胞、活化的T细胞和骨髓基质细胞产生。成熟的人IL-4与牛、小鼠和大鼠IL-4分别具有55%、39%和43%的氨基酸序列同一性。它是一种由T细胞产生的强效细胞因子，在较小程度上由肿瘤相关的自然杀伤细胞、嗜碱性粒细胞和肥大细胞产生。IL-4是分泌Th3的转化生长因子β的分化因子。IL-4通过信号转导子和转录激活子Stat6和一种名为4PS的170K蛋白的酪氨酸磷酸化激活两种不同的信号通路。

翌圣Rat IL-4 ELISA(酶联免疫吸附测定)试剂盒是一种体外酶联免疫吸附测定试剂盒，用于定量测定血清、血浆中的大鼠白介素4(Rat IL-4)。特异性抗大鼠白介素4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本，经过孵育，样本中存在的大鼠白介素4与固相抗体结合。洗涤去除未结合的物质后，加入检测抗体孵育结合，经洗涤，再加入酶结合物(Streptavidin-HRP)孵育结合。洗涤后，加入显色底物 TMB，避光显色。颜色反应的深浅与样本中大鼠白介素4的浓度成正比。加入终止液终止反应，在450 nm 波长(参考波长570 – 630 nm)测定吸光度值。

 

产品信息

货号

98503ES48 / 98503ES96 

规格

48 T / 96 T

检测范围

15.63-1000 pg/mL

检测方法

双抗夹心法

检测物种

大鼠

检测时长

4.5小时

灵敏度

11.6 pg/mL

稀释线性

85 – 117%

回收率

76 – 123%

板内差

2.0%

板间差

3.0%

 

实验数据

  1. 标曲数据

数据拟合绘制标准曲线，生成的标曲用于实验数据分析。

标准曲线图

浓度（pg/mL）

显色值

平均值

校准值

1000

2.821

2.716

2.768

2.777

500

2.148

2.029

2.088

2.056

250

1.322

1.245

1.283

1.326

125

0.802

0.775

0.788

0.767

62.5

0.432

0.414

0.423

0.423

31.25

0.252

0.243

0.247

0.238

15.625

0.145

0.142

0.144

0.147

0

0.060

0.058

0.059

/

Rat IL-4 ELISA Kit

  1. 灵敏度检测

IL-4的最低检测限为11.6 pg/mL，通过使用重复检测20次零孔OD值的均值与标准差计算得出。

  1. 精密度检测

酶标板内精密度

3个已知浓度的样本酶标板内重复测定6次，评估酶标板内的精密度。

酶标板间精密度

3个已知浓度的样本酶标板间重复检测22次，评估酶标板间的精密度。

项目

板内精密度

板间精密度

样本

1

2

3

1

2

3

6

6

6

22

22

22

平均值

468.10

122.28

32.29

468.08

123.19

32.10

标准差

12.47

2.48

0.43

15.75

2.68

1.07

变异系数（%）

2.7

2.0

1.3

3.4

2.2

3.3
  1. 回收率检测

 通过不同水平的IL-4添加于样本中来测定回收率，其回收率如下:

样本类型

平均回收率(%)

范围（%）

血清

82.4

75.7-89.5

血浆

118.4

112.0-123.2

细胞培养上清

89.2

82.4-93.6
  1. 稀释线性检测

血清稀释比例

平均期望值(%)

范围（%）

1:02

99.0

94.8-105.1

1:04

96.8

95.3-99.6

1:08

104.7

103.3-105.8

1:16

110.0

105.7-116.7

 

血浆稀释比例

平均期望值(%)

范围（%）

1:02

98.7

95.1-104.8

1:04

100.1

94.5-107.5

1:08

103.6

97.5-106.9

1:16

92.4

85.2-104.5

 

细胞培养上清稀释比例

平均期望值(%)

范围（%）

1:02

100.0

97.4-101.9

1:04

101.3

96.8-104.4

1:08

107.2

100.9-112.9

1:16

104.7

94.8-112.7
  1. 样本值

应用本试剂盒，检测若干健康志愿者的样本，志愿者的用药史不详。

样本类型

样本数目

平均值（pg/mL）

样本值（pg/mL）

血清

10

n.d.

n.d.

血浆

8

n.d.

n.d.

细胞培养上清

4

n.d.

n.d.

n.d. 指样本浓度值低于检测范围内得15.63 pg/mL

  1. 测定特异性

本试剂盒识别天然和重组大鼠白介素4。下述因子进行了特异性的评估，没有观察到明显的交叉反应和干扰影响。

Recombinant Rat:

Recombinant Human:

Recombinant Mouse:

IL-1α

IL-10

IL-4

IL-4

IL-1β

IL-18

 

 

IL-2

IFN-γ

 

 

IL-6

TNF-α

 

 

检测简图

Rat IL-4 ELISA Kit

 

疑问解答

问题

造成原因

解决方法

标曲不好

移液量不准确

检查移液器，按时校准，仔细操作，充分混匀时盖紧管口并尽量避免泡沫。

稀释方法不恰当

显色值偏低

孵育时间太短

给予足够的孵育时间，样本和溶解的标准品隔夜后更换。

移液量不足或稀释不当

校准移液器和规范操作

CV偏高

酶标板清洗不当

使用正确的洗涤工序；如果使用洗板机，检查所有的端口是否堵塞。

受污染的洗液

准备新鲜洗液

灵敏度较低

试剂盒储存不当

按照产品组分表进行保存。

 

Ver.CN20231106

 

Rat IL-4 ELISA Kit

暂无内容

Rat IL-4 ELISA Kit

暂无内容

Bio-Serv动物模型饲料K3170 Rat Retreat, Blue, Certified

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Bio-Serv动物模型饲料K3170 Rat Retreat, Blue, Certified

K3170

K3245

K3246

Product Details
  • Certified (Contaminant Screened)
  • Made of high temp polycarbonate
  • Best rat enrichment ever!
  • Allows for sheltering and gnawing opportunities
  • Flat top allows for increased floor space
  • Large flat sides prevent rolling
  • Autoclavable
  • Cagewasher safe
Dimensions
3 3/4″ wide x 3 3/4″ high x 6″ long
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