重组食蟹猴PTK7/CCK4蛋白(His标签) Cynomolgus PTK7/CCK4 Protein

重组食蟹猴PTK7/CCK4蛋白(His标签) Cynomolgus PTK7/CCK4 Protein

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

PTK7;CCK-4;

表达区间及表达系统(Source)

Cynomolgus PTK7/CCK4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala39-Thr712.[Accession | A0A2K5VNI5]

分子量大小(Molecular Weight)

The protein has a predicted MW of 75.73 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组食蟹猴PTK7/CCK4蛋白(His标签) Cynomolgus PTK7/CCK4 Protein

暂无内容

重组食蟹猴PTK7/CCK4蛋白(His标签) Cynomolgus PTK7/CCK4 Protein

暂无内容

 

性能参数

分子别名(Synonyms)

PTK7;CCK-4;

表达区间及表达系统(Source)

Cynomolgus PTK7/CCK4 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala39-Thr712.[Accession | A0A2K5VNI5]

分子量大小(Molecular Weight)

The protein has a predicted MW of 75.73 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组食蟹猴PTK7/CCK4蛋白(His标签) Cynomolgus PTK7/CCK4 Protein

暂无内容

重组食蟹猴PTK7/CCK4蛋白(His标签) Cynomolgus PTK7/CCK4 Protein

暂无内容

Clontech pTK-hyg (VSC0467)说明书

 

pTK-hyg

型号 载体名称 出品公司 载体用途
VSC0467 pTK-hyg Clontech 四环素调控系统

 

Description

pTK-Hyg is a selection vector that confers hygromycin resistance in mammalian cells for the 

selection of stably transformed cells. pTK-Hyg is especially useful for selection of double-stable 

cell lines using the Tet-On® or Tet-Off® Gene Expression Systems because the absence of an 

enhancer element on pTK-Hyg prevents the unwanted activation of pTRE- and pBI-derived 

plasmids upon cointegration into the host cell’s genome.

Use

pTK-Hyg can be cotransfected into the host cell line with an expression plasmid that contains 

a gene of interest using any standard transfection technique. Most mammalian cell lines 

require 200 µg/ml of Hygromycin to select for stably transformed cells.

Location of Features

HSV TK polyA signals: 1473–1478 & 1486–1491 (complementary)

Hygr

 (hygromycin resistance gene): 1537–2574 (complementary)

P

HSV TK (HSV TK promoter): 2588–2835 (complementary)

pUC origin ofreplication: 3152–3795

Ampr

 (ampicillin resistance gene; beta-lactamase): 3943–4803

Propagation in E. coli

Suitable host strains: DH™ and other general purpose strains. 

• Selectable marker: plasmid confers resistance to ampicillin (50 µg/ml) on E. coli hosts.

• E. coli replication origin: pUC

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