标签归档：psilencer

pSilencer 2.1-U6 hygro载体说明书

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pSilencer 2.1-U6 hygro

载体描述: 

This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene （hygromycin） to enable selection of transfected cells and features the human RNA polymerase III promoter （U6）. Sufficient reagents are provided for 20 reactions.

• Select transfected cells to enrich the population of cells expressing your siRNA 
• Eliminate the need to synthesize RNA oligonucleotides for RNAi experiments
• Supplied linearized and ready for ligation

Compensate for Low Transfection Efficiencies and Perform Long-Term Studies
The use of mammalian siRNA expression vectors with antibiotic selectable markers conveys many benefits. Selectable markers can help compensate for poor plasmid transfection efficiencies seen with some cell lines. In these cases, only a fraction of the transfected cells express the siRNA, and reduction in target gene expression with even a potent siRNA can be difficult to detect. Use of a selectable marker and transient antibiotic selection permits.

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pSilencer 4.1-CMV puro载体说明书

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pSilencer 4.1-CMV puro

载体描述: 

The pSilencer 4.1-CMV siRNA Expression vectors are linearized with both BamH 1 and Hind III to facilitate directional cloning. They are also purified to remove the digested insert so that it cannot re-ligate with the vector. This greatly increases the percentage of clones bearing the hairpin siRNA template insert after ligation, reducing the time and effort required to screen clones.

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pSilencer 3.1-H1 hygro载体说明书

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pSilencer 3.1-H1 hygro

型号 载体名称 出品公司 载体用途
VRA0340 pSilencer 3.1-H1 hygro Ambion RNAi载体
载体描述: 

The pSilencer vectors employ RNA polymerase III （pol III） promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.

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pSilencer 3.1-H1 neo载体说明书

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pSilencer 3.1-H1 neo

型号 载体名称 出品公司 载体用途
VRA0341 pSilencer 3.1-H1 neo Ambion RNAi载体
载体描述: 

The pSilencer vectors employ RNA polymerase III （pol III） promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.

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pSilencer1.0载体说明书

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 pSilencer1.0

型号 载体名称 出品公司 载体用途
VRA0337 pSilencer1.0 Ambion RNAi载体

One of the biggest challenges in the use of RNA interference （RNAi） to

study gene function in mammalian systems is delivering the small interfering RNAs （siRNA） or siRNA expression vector into cells. The

pSilencer™ ade.0-CMV System employs adenoviral mediated gene

transfer—a well characterized and useful tool for the delivery of DNA

sequences both in vivo and in vitro. Once inside a mammalian cell, a

modified CMV promoter drives the expression of a hairpin siRNA for

RNAi studies. Using this kit, researchers can produce a replication-deficient adenovirus containing a hairpin siRNA template that targets their

gene of interest. It has been shown that adenoviral vectors are capable of

expressing functional siRNAs that enter the RNAi pathway and reduce

target gene expression in both tissue culture cells and animals （Xia 2002）.

 

pSilencer5.1-H1 Retro载体说明书

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 RNAi载体，pSilencer5.1-H1 Retro载体

pSilencer5.1-H1 Retro

型号 载体名称 出品公司 载体用途
VRA0581 pSilencer5.1-H1 Retro Ambion RNAi载体
载体描述: 

pSilencer 5.1 Retro vectors harness retroviruses’ ability to stably integrate into the host genome of nearly all mitotically active cells and include a puromycin resistance gene for selection of cells that express the recombinant DNA. The pSilencer 5.1 Retro vectors enable researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. This technology expands the capabilities of RNA interference research to address long-term alteration of gene expression.
pSilencer 5.1-H1 Retro uses the H1 promoter.

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pSilencer5.1-U6 Retro RNAi载体说明书

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 Ambion RNAi载体，RNAi载体，pSilencer5.1-U6 Retro载体

pSilencer5.1-U6 Retro

型号 载体名称 出品公司 载体用途
VRA0580 pSilencer5.1-U6 Retro Ambion RNAi载体
载体描述: 

pSilencer 5.1 Retro vectors harness retroviruses’ ability to stably integrate into the host genome of nearly all mitotically active cells and include a puromycin resistance gene for selection of cells that express the recombinant DNA. The pSilencer 5.1 Retro vectors enable researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. This technology expands the capabilities of RNA interference research to address long-term alteration of gene expression.
pSilencer 5.1-U6 Retro uses the U6 promoter.

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