标签归档：psicor

pSicoR载体序列

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 pSicoR载体说明书

型号 载体名称 出品公司 载体用途
VMA0372 pSicoR Addgene 慢病毒载体
产品参数: 

Backbone size （bp）: 7567
Modifications to Backbone: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5′: HpaI
Cloning site 3′: XhoI
5′ sequencing primer: mU6-F

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pSicoR allows for conditional （Cre-Lox）, stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression.

质粒图谱: 

pSicoR pGK puro载体

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 pSicoR pGK puro载体

型号 载体名称 出品公司 载体用途
VMA0373 pSicoR pGK puro Addgene 慢病毒载体
产品参数: 

Backbone size （bp）: 7700
Modifications to Backbone: Puromycin is expressed from this plasmid as a selectable marker. Puromycin and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5′: HpaI 
Cloning site 3′: XhoI
5′ sequencing primer: mU6-F

 

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pSicoR PGK Puro allows for conditional （Cre-Lox）, stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression. See pSicoR and pSico PGK Puro for more information.

The shRNA coding oligos have to be cloned into the HpaI and XhoI restriction sites. Oligo design information can be found on the author’s map for pSico and on the Jacks Lab 

质粒图谱: