产品描述

5×SDS-PAGE蛋白上样缓冲液(SDS-PAGE Sample Loading Buffer)，是一种经过改良的以溴酚蓝为染料，5倍浓缩的蛋白上样缓冲液。

SDS可与蛋白质结合使蛋白质-SDS复合物上带有大量的负电荷， 这时蛋白质本身的电荷完全SDS掩盖，消除了各种蛋白质本身电荷的差异， SDS还可以断开分子内和分子间的氢键，破坏蛋白质分子的二级和三级结构， DTT可以断开半胱氨酸残基之间的二硫键， 破坏蛋白质的空间结构， 消除了蛋白结构之间的差异。因此，电泳速度只是与其分子量大小有关。溴酚蓝用作电泳时的指示剂， 可大概指示电泳结束的时间。

可以用于常规的SDS-PAGE蛋白样品的上样。

运输与保存方法

冰袋运输。 -20℃储存，一年有效期。

注意事项

1)为了您的安全和健康，请穿实验服并戴一次性手套操作。

2)5×SDS-PAGE蛋白上样缓冲液中含少量DTT，有轻微刺激性气味，但不含剧毒的巯基乙醇。

3)5×SDS-PAGE蛋白上样缓冲液必须完全溶解后再使用。

操作方法

1)在室温或不超过37℃的水浴中溶解5×SDS-PAGE上样缓冲液。水浴溶解后立即室温存放， 尽量避免长时间置于水浴中。

2)按照每4 μL蛋白样品加入1 μL 5×SDS-PAGE蛋白上样缓冲液的比例， 混合蛋白样品和5×SDS-PAGE蛋白上样缓冲液。

3)100℃或沸水浴加热3-5分钟，以充分变性蛋白。

4)冷却到室温后，直接上样到SDS-PAGE胶加样孔内即可。

5)通常电泳至蓝色染料到达胶的底端处附近即可停止电泳。

6)本产品仅作科研用途！

HB220402

Q：该产品有沉淀，可以直接使用吗？

A：5×SDS-PAGE 蛋白上样缓冲液必须完全溶解后再使用。

Q：该产品有沉淀，能直接煮沸溶解吗？

A：不可以。在室温或不超过 37°C 的水浴中溶解 5×SDS-PAGE 上样缓冲液。水浴溶解后立即室温存放，尽量避免长时间置于水浴中。

Q：蛋白自身的电荷会对缓冲液有影响吗？

A：不会的。5×SDS-PAGE 蛋白上样缓冲液中 SDS 可与蛋白质结合使蛋白质－SDS 复合物上带有大量的负电荷，这时蛋白质本身的电荷完全被 SDS 掩盖，因此，电泳速度只是与其分子量大小有关。

Q：上样过程需要添加指示剂吗？

A：需要，一般采用溴酚蓝用作电泳指示剂，可大概指示电泳结束的时间。

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