产品描述

LDL是由极低密度脂蛋白（VLDL）转变而来，主要功能是把胆固醇运输到全身各处细胞，运输到肝脏合成胆酸，其可用于研究受体介导的内吞作用过程，尤其是在动脉粥样硬化等疾病中，其血浆来源的LDL可用于研究LDL在功能和代谢中的氧化作用。

氧化的LDL（Ox-LDL）是修饰LDL中的一类。修饰的LDL除包括氧化修饰的LDL外，还包括乙酰化LDL及丙二醛（MDA）、4-羟烯酸（4-HNE）直接结合的LDL，这些未经氧化修饰而仅经一般化学修饰的LDL称为衍化的LDL。不同于衍化的LDL，Ox-LDL的生理学独特性表现在：1）在细胞生理功能影响上，Ox-LDL可影响花生四烯酸的代谢，抑制胆固醇酯化作用等，但衍化的LDL无上述效应；2）Ox-LDL消耗LDL内源性抗氧化物质，使LDL上的维生素E含量下降，而MDA-LDL无上述效应；3）氧化修饰涉及脂质过氧化反应，LDL中的PUFAs被氧化。MDA对LDL修饰，是直接和ApoB-100结合成希夫氏碱，脂质过氧化反应轻微；4）氧化LDL在氧化程度低时，ApoB降解；在氧化程度高时，ApoB又可发生再聚合。MDA对LDL的修饰，ApoB无降解、聚合反应发生；5）Ox-LDL产生的荧光峰波长为430 nm，而MDA -LDL的荧光峰波长为460 nm。Ox-LDL不经LDL受体代谢，由清道夫受体识别、结合、内吞饮入细胞并丧失正常的胆固醇代谢途径，引起细胞内脂质沉积，泡沫样变。

LDL氧化修饰的方式有很多种，常见的有：1）细胞介导的LDL氧化修饰，又称为生物氧化修饰的LDL。如内皮细胞，巨噬细胞，单核细胞都具有此功能；2）过度金属离子介导的LDL氧化修饰，如Ca²⁺，Fe²⁺等；还有其他形式的氧化修饰，包括物理方法如紫外线，或过氧化物酶催化。

YEASEN提供的人源氧化低密度脂蛋白（Human Oxidized Low Density Lipoprotein，Ox-LDL），是由过度铜离子介导人血浆来源的LDL进行的氧化修饰。新鲜血浆经检测为HCV，HBsAg和HIV阴性。本产品为无菌包装，可以直接稀释使用。本Ox-LDL广泛用于脂质代谢的研究，较少诱导细胞凋亡。另外我们还提供High Ox-LDL（货号：20608JP03）可产生明显的氧化应激，能够用来诱导细胞凋亡，并建立细胞损伤模型。除提供Ox-LDL，我们还提供人源乙酰化LDL（Ac-LDL），以及荧光标记的LDL。 

制备方法

在含10 μM Cu₂SO₄的PBS溶液中氧化人LDL，加入过量的EDTA终止氧化反应。

产品性质

稀释方法

根据实验需要用PBS磷酸盐缓冲液或细胞培养液稀释即可。

运输与保存方法

冰袋运输。4℃保存，建议避光，保存时间不要超过6周。千万不可冻存!!

注意事项

1）该产品经长期保存后会看到少量沉淀，属于正常现象。低速离心1 ~2 min去除沉淀物，得到澄清液。

2）Ox-LDL工作液很不稳定，强烈建议根据单次需要用量，新鲜配置工作液。

3）为了您的安全和健康，请穿实验服并戴一次性手套操作。

4）本产品仅作科研用途！

HB210830

Q：该产品与 20604 区别是什么？

A：该产品主要研究 LDL 在功能和代谢中的氧化作用；而 20604 主要研究乙酰化作用。

Q：该产品的缓冲液是多少？

A：10 μM Cu2SO4 的PBS 溶液。

Q：如何终止产品的氧化反应？

A：加入过量的 EDTA。

Q：该产品长期保存会出现少量沉淀，还可以使用吗？

A：这是正常现象。低速离心 1 ~2min 去除沉淀物，即得到澄清液。

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