New England Biolabs(NEB)公司介绍
简要描述:NEB公司成立于二十世纪七十年代中期,拥有众多经验丰富的科学家,是生产生命科学试剂的*。目前,NEB为基因组研究提供Z齐全的重组酶和天然酶,并且公司业务范围已延伸至蛋白质组学和药品开发领域。
详细介绍
产品咨询
公司:http://www.neb.com
简要描述:NEB公司成立于二十世纪七十年代中期,拥有众多经验丰富的科学家,是生产生命科学试剂的*。目前,NEB为基因组研究提供Z齐全的重组酶和天然酶,并且公司业务范围已延伸至蛋白质组学和药品开发领域。
详细介绍
产品咨询
公司:http://www.neb.com
New england biolabs
NEB公司成立于二十世纪七十年代中期,拥有众多经验丰富的科学家,是生产生命科学试剂。目前,NEB为基因组研究提供的重组酶和天然酶,并且公司业务范围已延伸至蛋白质组学和药品开发领域。
NEB公司成立于二十世纪七十年代中期,拥有众多经验丰富的科学家,是生产生命科学试剂。目前,NEB为基因组研究提供的重组酶和天然酶,并且公司业务范围已延伸至蛋白质组学和药品开发领域。
Founded in the mid-1970s by scientists committed to developing innovative products for the life sciences, New England Biolabs is a recognized world leader in the discovery, development and commercialization of reagents for genomic research.
产品列表:
No. |
品牌 |
货号 |
名称 |
规格 |
1 |
New england biolabs |
E6350S |
NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads |
6 reactions |
2 |
New england biolabs |
M0492S |
Q5® High-Fidelity 2X Master Mix |
100 reactions |
3 |
New england biolabs |
M0205S |
E. coli DNA Ligase |
200 units |
4 |
New england biolabs |
R0734S |
Esp3I |
300 units |
5 |
New england biolabs |
T1020S |
Monarch® DNA Gel Extraction Kit |
50 preps |
6 |
New england biolabs |
E0546S |
BioBrick® Assembly Kit |
50 reactions |
7 |
New england biolabs |
M0386S |
Cas9 Nuclease, S. pyogenes |
70 pmol |
8 |
New england biolabs |
C1010S |
Cloning Competent E.coli Sampler |
8 tubes |
9 |
New england biolabs |
N3233S |
Low Molecular Weight DNA Ladder |
0.1 mL |
10 |
New england biolabs |
E1610S |
EpiMark® N6-Methyladenosine Enrichment Kit |
20 reactions |
11 |
New england biolabs |
E9100S |
SNAP-Cell® Starter Kit |
1 set |
12 |
New england biolabs |
P0763S |
E. coli Ribosome |
1 mg |
13 |
New england biolabs |
M0649S |
Nucleoside Digestion Mix |
50 reactions |
14 |
New england biolabs |
P8109S |
Endoproteinase LysC |
20μg |
15 |
New england biolabs |
E8033S |
Anti-M13 pIII Monoclonal Antibody |
0.1 mL |
简要描述:NEB代理,NEB中国,NEB代理,NEB公司,NEB专业代理-上海金畔生物科技有限公司,具体产品信息欢迎电询:021-50837765
详细介绍
产品咨询
世界*实验材料供应商NEB上海金畔生物为其中国代理,NEB 在一直是行业的*,一直为广大科研客户提供zui为优质的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, NEB 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
NEB 中国代理,NEB 上海代理,NEB北京代理,NEB 广东代理,NEB 江苏代理 NEB 湖北代理, NEB 天津, NEB 黑龙江代理, NEB内蒙古代理, NEB 吉林代理, NEB 福建代理, NEB 江苏代理, NEB 浙江代理, NEB 四川代理,
美国纽英伦生物技术NEB New England Biolabs www.neb-china.com www.neb.com
New England Biolabs, Inc.成立于20世纪70年代,致力于生化酶类及其它相关试剂的研发和生产。经过20多年的不懈努力,New England Biolabs, Inc.(NEB)凭借其高品质的产品、*的专业服务,已成为限制性内切酶及相关产品供应商之一。到目前为止,NEB可提供210多种内切酶,其中有130多种内切酶为重组酶。克隆及重组技术的运用,使NEB能在提高内切酶的纯度及品质的同时,极大地削减生产费用,从而为用户提供价格更合理、品质更优良的产品。NEB将通过不懈努力,在内切酶及相关领域继续保持先进地位,为客户提供更多更好的产品和更专业的服务。
美国NEB作为zui早生产商品化限制性内切酶的公司之一,在分子生物学的限制性内切酶及其相关产品方面处于世界,可以提供1739多种限制性内切酶和多种与DNA或蛋白修饰相关的重组酶.主要产品包括限制性内切酶,聚合酶,修饰酶,各种载体, Marker , 引物,测序,基因突变系统,噬菌体呈现,蛋白质工具(蛋白激酶,磷酸酶,糖生物学,蛋白酶)。该公司产品线主要分为以下九大方面:①限制性内切酶;②聚合酶与扩增技术;③DNA修饰酶与克隆技术;④核酸;⑤RNAi与RNA酶;⑥基因表达和蛋白纯化技术;⑦感受态细胞;⑧蛋白质工具;⑨表观遗传学。
Established in the mid-1970s as a cooperative laboratory of experienced scientists, New England Biolabs is a world leader in the production and supply of reagents for the life science industry. NEB now offers the largest selection of recombinant and native enzymes for genomic research and continues to expand its product offerings into areas related to proteomics and drug discovery.
After more than 30 years, NEB can be considered one of the first companies to help shape today’s biotechnology industry. Housed in a new state-of-the-art facility, NEB’s USA headquarters includes a modern fermentation center and fully equipped laboratories for production, quality control, product development and basic research. As one of the first companies to produce restriction enzymes on a commercial scale, NEB continues to specialize in restriction enzymes and consistently maintains a position at the forefront of this field. At New England Biolabs, science has always been a priority and through this approach, NEB’s reagents have gained a world-wide reputation for setting the highest of standards for quality and value.
Recombinant Enzymes
At NEB, enzyme production is linked to basic research in the cloning and overexpression of restriction/modification enzyme systems. This enables us to introduce substantial cuts in reagent costs and unsurpassed improvements in product quality. At NEB, over 160 enzyme systems have been cloned, many compley and some partially. Presently, NEB supplies more than 240 restriction enzymes, over 160 of which are available in recombinant form, as well as numerous recombinant polymerases and modifying enzymes for a wide variety of applications.
Quality and Customer Service
NEB is dedicated to providing research products of the highest purity to the worldwide scientific community and supports this dedication with extensive quality control testing, a commitment to customer service and an intensive research and development program. When you contact NEB either directly or via one of our International offices you will notice our personalized approach to customer service. The same scientists (product managers) who purify and quality-control our enzymes respond to questions concerning specific restriction endonucleases, methylases, other DNA modifying enzymes and protein modifying enzymes. Our Organic Synthesis Division provides information about linkers, primers, adaptors, probes and custom oligonucleotide synthesis, and our research staff is available as a technical resource to our customers. These staff scientists have expertise in DNA sequencing, methylation, cloning, over-expression, fermentation, protein purification and protein analysis.
Basic Science
Basic research at NEB, in Molecular Biology and Parasitology, is supervised by our senior scientists. Well established in their respective fields, the scientists at NEB routinely publish their work in the leading journals, supervise post-doctoral fellows, offer a number of summer internships to undergraduate students and are regularly invited to lecture at local high schools. Collaborations are encouraged outside of NEB and a weekly academic seminar series enables scientists to visit our facility and interact with our staff.
Environmental Policy
One of the founding principles of NEB was to do everything possible to promote sound ecological practices. Products are prepared, analyzed and shipped to you under conditions compatible with both product stability and our environmental policy. Most prominent in our efforts is our shipping box recycling program, established in the USA over 20 years ago and now maintained in Canada, Germany and the United Kingdom. We also have a comprehensive in-house recycling program and regularly print our marketing literature on recycled paper. These small but important steps do not compromise the quality of our products; the benefit is clearly environmental.
Corporate Responsibility
In addition to our sound environmental policy, NEB seeks to improve the quality of life in our local community and around the world. Much of this effort is achieved through the New England Biolabs Foundation, a private foundation established in 1982, that supports environmental awareness, education, health and the arts, primarily in developing countries. Closer to home, projects in our local community are also supported through our corporate donations committee.
International Network of New England Biolabs
The International distribution of NEB’s products is achieved through a worldwide network of distributors, affiliates and NEB owned subsidiaries, all committed to providing quality, value and service. The countries serviced by this International Network include: Argentina, Australia, Austria, Belgium, Brazil, Canada, China, Denmark, Federal Republic of Germany, Finland, France, Greece, Hong Kong, Iceland, India, Republic of Ireland, Israel, Italy, Japan, Korea, Luxembourg, Malaysia, Mexico, The Netherlands, New Zealand, Norway, Portugal, Singapore, Spain, Sweden, Switzerland, Taiwan, Thailand and the United Kingdom. Countries not included in this International Network please contact NEB headquarters in the USA for further information.
上海金畔生物科技有限公司
neb2024年价格表
NEB成立于二十世纪七十年代中期,拥有众多经验丰富的科学家,致力于生命科学领域高质量产品的开发和创新。NEB以成为全球范围内分子生物应用酶的开发和生产领d者感到自豪。
NEB公司的创立,源于“scientists for scientists”理念,将科学进步置于s位,注重环境保护,以回报我们的世界。自1974年成立伊始,我们一直致力于高质量产品的开发和创新,持续助力与赋能科学研究,也不断成就自我。NEB坚持将盈收广泛投入到科学研究项目中,以此与客户形成紧密连接并推动科学突破。从我们的创立原则到我们d一无二的企业文化,NEB的企业哲学可以提炼为三个核心价值观:激情、谦逊和存真。
为基因组研究提供种类z齐全的重组酶和天然酶。限制性内切酶一直是我们的核心产品,同时我们也在不断扩大其他产品线,这些产品线包括了PCR,基因表达,NGS文库制备,合成生物学,糖生物学,表观遗传学和RNA分析相关工具。除此之外,我们也更专注于加深合作,借此将新技术延伸到分子诊断开发和核酸疫苗等关键市场领域。
特别告知:为保证产品质量与良好性能,所以在购买相应产品的同时我司会收取一定量的运输费用!!!本次报价有效时间为2024年1月1日—2024年12月14日。
货号 | 品名 | 规格 | 价格 | 品牌 | 货期 | 价格来源 |
P0704L | PNGase F | 75,000units | 8469 | neb | 1周 | 上海金畔生物 |
P0705L | PNGase F (Glycerol-free) | 75,000units | 8469 | neb | 1周 | 上海金畔生物 |
R3101S | EcoRI-HF | 10000units | 699 | neb | 1周 | 上海金畔生物 |
R3552S | AgeI-HF | 300units | 909 | neb | 1周 | 上海金畔生物 |
M0202L | T4 DNA Ligase – 100000 units | 100,000units | 3189 | neb | 1周 | 上海金畔生物 |
R0145S | XbaI | 3000units | 849 | neb | 1周 | 上海金畔生物 |
R3136S | BamHI-HF | 10000units | 699 | neb | 1周 | 上海金畔生物 |
R3189S | NotI-HF | 500units | 899 | neb | 1周 | 上海金畔生物 |
R3150L | PvuI-HF | 2500units | 3759 | neb | 1周 | 上海金畔生物 |
M0202S | T4 DNA Ligase – 20000 units | 20,000units | 809 | neb | 1周 | 上海金畔生物 |
R0146V | XhoI | 2500units | 479 | neb | 1周 | 上海金畔生物 |
R0146S | XhoI | 5000units | 859 | neb | 1周 | 上海金畔生物 |
N0362S | ssRNA Ladder | 25gellanes | 909 | neb | 1周 | 上海金畔生物 |
R3104S | HindIII-HF | 10000units | 699 | neb | 1周 | 上海金畔生物 |
M0525S | Quick CIP – 1000 units | 1,000units | 1199 | neb | 1周 | 上海金畔生物 |
M0297S | RNase H – 250 units | 250units | 879 | neb | 1周 | 上海金畔生物 |
R3131S | NheI-HF | 1000units | 849 | neb | 1周 | 上海金畔生物 |
E2040S | HiScribe T7 High Yield RNA Synthesis Kit – 50 reactions | 50rxns | 2759 | neb | 1周 | 上海金畔生物 |
R3193S | NcoI-HF | 1000units | 789 | neb | 1周 | 上海金畔生物 |
R3133S | SpeI-HF | 500units | 849 | neb | 1周 | 上海金畔生物 |
R3539L | BbsI-HF | 1500units | 3869 | neb | 1周 | 上海金畔生物 |
R3733S | BsaI-HFv2 | 1000units | 899 | neb | 1周 | 上海金畔生物 |
R0607S | Nt.BstNBI | 1000units | 909 | neb | 1周 | 上海金畔生物 |
E2621L | NEBuilder HiFi DNA Assembly Master Mix – 50 reactions | 50rxns | 7029 | neb | 1周 | 上海金畔生物 |
R0176S | DpnI | 1000units | 849 | neb | 1周 | 上海金畔生物 |
R3138S | SalI-HF | 2000units | 759 | neb | 1周 | 上海金畔生物 |
N0445L | 7-deaza-dGTP – 1.5 umol | 1.5umol | 3629 | neb | 1周 | 上海金畔生物 |
B0201S | T4 Polynucleotide Kinase Reaction Buffer | 4.0ml | 399 | neb | 1周 | 上海金畔生物 |
B0202S | T4 DNA Ligase Reaction Buffer | 5ml | 399 | neb | 1周 | 上海金畔生物 |
B0208S | Taq DNA Ligase Reaction Buffer | 6.0ml | 399 | neb | 1周 | 上海金畔生物 |
B0216L | T4 RNA Ligase Reaction Buffer | 3.0ml | 739 | neb | 1周 | 上海金畔生物 |
B0289S | Antarctic Phosphatase Reaction Buffer | 6.0ml | 399 | neb | 1周 | 上海金畔生物 |
B0293S | Exonuclease I Reaction Buffer | 6.0ml | 399 | neb | 1周 | 上海金畔生物 |
B0303S | DNase I Reaction Buffer | 6.0ml | 379 | neb | 1周 | 上海金畔生物 |
B0349S | NEBNext dsDNA Fragmentase Reaction Buffer v2 – 6 ml | 6.0ml | 509 | neb | 1周 | 上海金畔生物 |
B0363S | RNA Loading Dye (2x) | 4.0ml | 709 | neb | 1周 | 上海金畔生物 |
B0374S | Isothermal Amplification Buffer II Pack | 6.0ml | 449 | neb | 1周 | 上海金畔生物 |
B0518S | Phusion® HF Buffer Pack | 6.0ml | 399 | neb | 1周 | 上海金畔生物 |
B0519S | Phusion® GC Buffer Pack | 6.0ml | 399 | neb | 1周 | 上海金畔生物 |
B0535S | StickTogether™ DNA Ligase Buffer | 2ml | 669 | neb | 1周 | 上海金畔生物 |
B0537S | Isothermal Amplification Buffer Pack | 6.0ml | 469 | neb | 1周 | 上海金畔生物 |
B0701S | Endoglycosidase Reaction Buffer Pack | 4.0ml | 379 | neb | 1周 | 上海金畔生物 |
B1003S | Magnesium Sulfate (MgSO4) Solution | 6.0ml | 379 | neb | 1周 | 上海金畔生物 |
B1430S | NEBNext Adaptor Dilution Buffer – 9.6 ml | 9.6ml | 549 | neb | 1周 | 上海金畔生物 |
B1500L | Nuclease-free Water | 100ml | 879 | neb | 1周 | 上海金畔生物 |
B1500S | Nuclease-free Water | 25ml | 359 | neb | 1周 | 上海金畔生物 |
B1700S | LAMP Fluorescent Dye – 250 ul | 0.25ml | 729 | neb | 1周 | 上海金畔生物 |
B6001V | NEBuffer™ r1.1 | 2X1.25ml | 229 | neb | 1周 | 上海金畔生物 |
B6002S | NEBuffer™ r2.1 | 4×1.25ml | 399 | neb | 1周 | 上海金畔生物 |
B6002V | NEBuffer™ r2.1 | 2X1.25ml | 229 | neb | 1周 | 上海金畔生物 |
关键词:neb、重组酶、天然酶、限制性内切酶、RNA分析
neb P0704L说明书
PNGase F.
目录 #
P0704S
浓度
500,000单位/毫升
尺寸
P0704L 75,000个单位
价格表
$ 718.00
PNGase F是从糖蛋白中去除几乎所有N-连接的寡糖的有效的酶促方法。PNGase F是酰胺酶,其在高甘露糖,杂合和复合寡糖的内部GlcNAc和天冬酰胺残基之间切割。
肽 – N-糖苷酶F,也称为PNGase F,是一种酰胺酶,可裂解来自N-连接糖蛋白的高甘露糖,杂合寡糖和复合寡糖的内层GlcNAc和天冬酰胺残基(1)
详细的特异性:
当内部的GlcNAc残基与α1-3岩藻糖残基连接时,PNGase F不能从糖蛋白切割N-连接的聚糖。这种修饰常见于植物和一些昆虫糖蛋白中。
产品来源
PNGase F从脑膜炎黄杆菌(Flavobacterium meningosepticum)(3)中纯化,不含蛋白酶和Endo F活性。
提供的试剂
本产品随附以下试剂:
存储在(°C) | 浓度 | |
糖蛋白变性缓冲液 | -20 | 10 X. |
NP-40 | -20 | 10% |
GlycoBuffer 2 | -20 | 10 X. |
应用功能
单位定义
一个单位定义为在37℃下在10μl的总反应体积中在1小时内从10μg变性的RNase B中除去> 95%的碳水化合物所需的酶量。
单位定义测定:
将10μgRNaseB用1X糖蛋白变性缓冲液(0.5%SDS,40mM DTT)在100℃变性10分钟。加入NP-40和GlycoBuffer 2后,加入两倍稀释的PNGase F,将反应混合物在37℃下温育1小时。通过SDS-PAGE显现反应产物的分离。
1X糖蛋白变性缓冲液
0.5%SDS
40 mM DTT
1X NP-40
1%NP-40,MilliQ-H 2 O
反应条件
1X GlycoBuffer 2
在37°C孵育
1X GlycoBuffer 2
50 mM磷酸钠
(pH值@ 25°C)
贮存温度
-20℃下
储藏条件
在 25℃下,20mM Tris-HCl
50mM NaCl
5mM EDTA
50%甘油
pH 7.5
热灭活
75°C,10分钟
分子量
表观:36000道尔顿
查看有关Bioz的更多详细信息
目录 # | 专注 | 尺寸 | 价格表 |
---|---|---|---|
M0302S | 10,000单位/毫升 | 250伙 | $ 71.00 |
M0302L | 10,000单位/毫升 | 1,250单位 | $ 284.00 |