COXIV鼠单克隆抗体 COXIV Mouse mAb

COXIV鼠单克隆抗体 COXIV Mouse mAb

产品说明书

FAQ

COA

已发表文献

细胞色素c氧化酶(COX)是线粒体呼吸链的末端酶。 它是一种多亚基酶复合物,将电子从细胞色素c转移到分子氧,并在线粒体内膜上形成质子电化学梯度。该复合物由13个线粒体和核编码亚基组成。 线粒体编码的亚基进行电子转移和质子泵浦活动。核编码亚基的功能尚不清楚,但它们可能在调控和组装复合物中发挥作用。 该基因编码人类线粒体呼吸链酶的核编码亚基IV亚型1。 它位于NOC4(与COX4相邻的基因)的3’位,并与之共享一个启动子。 与该基因相关的假基因位于第13和14号染色体上。 选择性剪接导致多个转录变体编码不同的异构体。

 

产品性质

别名

COX4;COXIV;COX4-1.

SwissProt ID

P13073

基因ID

1327

免疫原

A synthetic peptide corresponding to carboxyl terminal residues of human COX IV

缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

分子量

17kDa

克隆类型

Monoclonal Antibody

亚型

IgG1

宿主

Mouse

物种反应

Human,Mouse,Rat,Hamster,Goat,Monkey

纯化方式

Affinity Purified

细胞定位

线粒体内膜

研究领域

内分泌代谢

 

应用

稀释比 WB: 1/1000 IHC: 1/50 ICC: 1/150 FC: 1/100 IP: 1/20

COXIV鼠单克隆抗体 COXIV Mouse mAb

Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (1:50 diluted),

showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0)

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.

 

 

运输与保存方法

冰袋运输-20存,1年有效期

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途!

HB220529

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

暂无内容

COXIV鼠单克隆抗体 COXIV Mouse mAb

暂无内容

细胞色素c氧化酶(COX)是线粒体呼吸链的末端酶。 它是一种多亚基酶复合物,将电子从细胞色素c转移到分子氧,并在线粒体内膜上形成质子电化学梯度。该复合物由13个线粒体和核编码亚基组成。 线粒体编码的亚基进行电子转移和质子泵浦活动。核编码亚基的功能尚不清楚,但它们可能在调控和组装复合物中发挥作用。 该基因编码人类线粒体呼吸链酶的核编码亚基IV亚型1。 它位于NOC4(与COX4相邻的基因)的3’位,并与之共享一个启动子。 与该基因相关的假基因位于第13和14号染色体上。 选择性剪接导致多个转录变体编码不同的异构体。

 

产品性质

别名

COX4;COXIV;COX4-1.

SwissProt ID

P13073

基因ID

1327

免疫原

A synthetic peptide corresponding to carboxyl terminal residues of human COX IV

缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

分子量

17kDa

克隆类型

Monoclonal Antibody

亚型

IgG1

宿主

Mouse

物种反应

Human,Mouse,Rat,Hamster,Goat,Monkey

纯化方式

Affinity Purified

细胞定位

线粒体内膜

研究领域

内分泌代谢

 

应用

稀释比 WB: 1/1000 IHC: 1/50 ICC: 1/150 FC: 1/100 IP: 1/20

COXIV鼠单克隆抗体 COXIV Mouse mAb

Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (1:50 diluted),

showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0)

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.

 

 

运输与保存方法

冰袋运输-20存,1年有效期

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途!

HB220529

 

COXIV鼠单克隆抗体 COXIV Mouse mAb

暂无内容

COXIV鼠单克隆抗体 COXIV Mouse mAb

暂无内容

重组小鼠MSLN/间皮蛋白(His标签) Mouse MSLN/Mesothelin Protein

重组小鼠MSLN/间皮蛋白(His标签) Mouse MSLN/Mesothelin Protein

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

CAK1; CAK1 antigen;MSLN; MPF

表达区间及表达系统(Source)

Mouse MSLN/Mesothelin Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp298-Ser600.[Accession | Q61468-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Mouse MSLN, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 19.4ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组小鼠MSLN/间皮蛋白(His标签) Mouse MSLN/Mesothelin Protein

暂无内容

重组小鼠MSLN/间皮蛋白(His标签) Mouse MSLN/Mesothelin Protein

暂无内容

 

性能参数

分子别名(Synonyms)

CAK1; CAK1 antigen;MSLN; MPF

表达区间及表达系统(Source)

Mouse MSLN/Mesothelin Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp298-Ser600.[Accession | Q61468-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Mouse MSLN, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 19.4ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组小鼠MSLN/间皮蛋白(His标签) Mouse MSLN/Mesothelin Protein

暂无内容

重组小鼠MSLN/间皮蛋白(His标签) Mouse MSLN/Mesothelin Protein

暂无内容

重组小鼠DLK1蛋白(His标签) Mouse DLK1 Protein

重组小鼠DLK1蛋白(His标签) Mouse DLK1 Protein

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

FA1;pG2; DLK

表达区间及表达系统(Source)

Mouse DLK1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala24-Gln305.[Accession | Q09163-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 30.9 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

FA1;pG2; DLK

表达区间及表达系统(Source)

Mouse DLK1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ala24-Gln305.[Accession | Q09163-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 30.9 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)

β-Actin Mouse Monoclonal Antibody
β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003) 一键复制产品信息

产品编号: AF0003

产品包装:100μl
选择包装

100μl

说明书下载

446.00 441.00 10

价格: ¥ 446.00

促销价: ¥ 446.00

促销价: ¥ 446.00 441.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
AF0003 β-Actin Mouse Monoclonal Antibody 100μl 446.00元
来源 用途 交叉反应性 分子量
Mouse WB, ICC, IHC, IF H, M, R 43KDa

WB,Western blot;IP,Immunoprecipitation;IF,Immunofluorescence;IHC,Immunohistochemistry;ICC,Immunocytochemistry;FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected. 
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ELISA ChIP
1:1000 1:20 1:20 1:20

抗体详细信息如下:

About this Antibody

Name

β-Actin Mouse Monoclonal Antibody

Category

Monoclonal antibody (mAb); Primary antibody

Isotype

IgG1

Purification

Affinity chromatography

About the Immunogen

Immunogen

synthetic peptide (KLH-coupled)

Gene ID

60/11461/81822

SwissProt

P60709/P60710/P60711

Synonyms

BRWS1; PS1TP5BP1

Category

Cytoskeletal Signaling

Background

Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error.

包装清单:

产品编号 产品名称 包装
AF0003 β-Actin Mouse Monoclonal Antibody 100μl
AZ100 Western一抗稀释液 100ml
说明书 1份

保存条件:
β-Actin Mouse Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。 
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
请根据抗体的实际用途选择相应的使用方法。
1. Western检测:

a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋
白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体 操作可以参考如下网页: http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:
β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)
Fig. 1. Western blot analysis on Hela (A), NIH/3T3 (B) and PC12 (C) cell lysatesusing β-actin mousemonoclonal antibody (AF003).
β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)
Fig. 2. Immunofluorescent staining of NIH/3T3 cells using β-actin mouse monoclonalantibody (AF0003). Nucleus (blue) were stained with DAPI.
β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)
Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissueusing β-actin mouse monoclonal antibody (AF0003).

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β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)
β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)

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β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)

β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)
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4. Zu-Guo Zheng, Si-Tong Zhu, Hui-Min Cheng, Xin Zhang, Gang Cheng, Pyone Myat Thu, Supeng Perry Wang, Hui-Jun Li, Ming Ding, Lei Qiang, Xiao-Wei Chen, Qing Zhong, Ping Li, Xiaojun Xu
Discovery of a potent SCAP degrader that ameliorates HFD-induced obesity, hyperlipidemia and insulin resistance via an autophagy-independent lysosomal pathway
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5. Yong Tang, Mengjia Hu, Yang Xu, Fang Chen, Shilei Chen, Mo Chen, Yan Qi, Mingqiang Shen, Cheng Wang, Yukai Lu, Zihao Zhang, Hao Zeng, Yong Quan, Fengchao Wang, Yongping Su, Dongfeng Zeng, Song Wang, Junping Wang
Megakaryocytes promote bone formation through coupling osteogenesis with angiogenesis by secreting TGF-β1
Theranostics. 2020 Jan 12;10(5):2229-2242.;doi: 10.7150/thno.40559. (IF 8.579)

6. Lianghua Shen, Sijia Lei, Bin Zhang, Shuaiguang Li, Luyuan Huang, Alexander Czachor, Mason Breitzig, Yuman Gao, Meiyan Huang, Xuemei Mo, Qing Zheng, Hanxiao Sun, Feng Wang
Skipping of exon 10 in Axl pre-mRNA regulated by PTBP1 mediates invasion and metastasis process of liver cancer cells
Theranostics. 2020 Apr 27;10(13):5719-5735.;doi: 10.7150/thno.42010. (IF 8.579)

7. Songling Hu, Lin Zhu, Yimeng Song, Xinrui Zhao, Qianping Chen, Yan Pan, Jianghong Zhang, Yang Bai, Haowen Zhang, Chunlin Shao
Radiation-induced abscopal reproductive effect is driven by TNF-α/p38 MAPK/Rac1 axis in Sertoli cells
Theranostics. 2021 Mar 31;11(12):5742-5758.;doi: 10.7150/thno.56853. (IF 8.579)

8. Xiu Yu, Shiqi Liang, Manqi Chen, Hanwen Yu, Ruiqi Li, Yuliang Qu, Xuhui Kong, Ruirui Guo, Rongyan Zheng, Zsuzsanna Izsvák, Chuanbo Sun, Mingzhu Yang, Jichang Wang
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9. Xiao Han, Jianbo Ni, Zengkai Wu, Jianghong Wu, Bin Li, Xin Ye, Juanjuan Dai, Congying Chen, Jing Xue, Rong Wan, Li Wen, Xingpeng Wang, Guoyong Hu
Myeloid-specific dopamine D 2 receptor signalling controls inflammation in acute pancreatitis via inhibiting M1 macrophage
BRIT J PHARMACOL. 2020 Jul;177(13):2991-3008.;doi: 10.1111/bph.15026. (IF 7.73)

10. Wei Yang, Shuai Liu, Yunlei Li, Yujie Wang, Yao Deng, Weimin Sun, Hualan Huang, Junmou Xie, Andong He, Honglv Chen, Ailin Tao, Jie Yan
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11. Tan Z, Zhang W, Sun J, Fu Z, Ke X, Zheng C, Zhang Y, Li P, Liu Y, Hu Q, Wang H, Zheng Z
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J NEUROINFLAMM. 2018 Sep 21;15(1):275. (IF 5.793)

12. Hou H, Yu X, Cong P, Zhou Y, Xu Y, Jiang Y
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CELL PROLIFERAT. 2019 Jul;52(4):e12617. (IF 5.753)

13. Sun H, Ou B, Zhao S, Liu X, Song L, Liu X, Wang R, Peng Z
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14. Xu J, Liu B, Ma S, Zhang J, Ji Y, Xu L, Zhu M, Chen S, Wu X, Wu D.
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CELL PHYSIOL BIOCHEM. 2018;45(4):1631-1640. (IF 5.5)

15. Zhu C, Jiang W, Cheng Q, Hu Z, Hao J
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CELL PHYSIOL BIOCHEM. 2018;46(2):644-653. (IF 5.5)

16. Zhu C, Jiang W, Cheng Q, Hu Z, Hao J.
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CELL PHYSIOL BIOCHEM. 2018 Mar 28;46(2):644-653. (IF 5.5)

17. Mengxiao Xie, Zhijiao Wu, Shuai Ying, Longfei Liu, Chenhui Zhao, Chunlei Yao, Zhiwei Zhang, Can Luo, Wenbo Wang, Dan Zhao, Jing Zhang, Wen Qiu, Yingwei Wang
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18. Qiong Li, Huifang Hou, Meng Li, Xia Yu, Hongbo Zuo, Jianhui Gao, Min Zhang, Zongjin Li, Zhikun Guo
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19. Song K, Yuan Y, Lin Y, Wang YX, Zhou J, Gai QJ, Zhang L, Mao M, Yao XX, Qin Y, Lu HM, Zhang X, Cui YH, Bian XW, Zhang X, Wang Y
ERBB3, IGF1R, and TGFBR2 expression correlate with PDGFR expression in glioblastoma and participate in PDGFR inhibitor resistance of glioblastoma cells.
Am J Cancer Res. 2018 May 1;8(5):792-809. eCollection 2018. (IF 5.177)

20. Zhang M, Linghu E, Zhan Q, He T, Cao B, Brock MV, Herman JG, Xiang R, Guo M.
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21. Yusi Guo, Xiaopei Chi, Yifan Wang, Boon Chin Heng, Yan Wei, Xuehui Zhang, Han Zhao, Ying Yin, Xuliang Deng
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22. Ding L, Wu J, Li D, Wang H, Zhu B, Lu W, Xu G.
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Mol Med Rep. 2016 Mar;13(3):2017-22. (IF 2.1)

23. Guo Q, Dong B, Nan F, Guan D, Zhang Y.
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Mol Med Rep. 2016 Jul;14(1):544-50. (IF 2.1)

24. Sun TL, Liu Z, Qi ZJ, Huang YP, Gao XQ, Zhang YY.
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25. Gao J, Zhang Y, Yu C, Tan F, Wang L.
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26. Wang Y, Che S, Cai G, He Y, Chen J, Xu W.
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27. Li XH, Deng YY, Li F, Shi JS, Gong QH.
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28. Liu B, Li F, Shi J, Yang D, Deng Y, Gong Q.
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29. Zhou J, Li Y, Yang L, Wu Y, Zhou Y, Cui Y, Yang G, Hong Y.
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30. Huang W, Liang Y, Dong J, Zhou L, Gao C, Jiang C, Chen M, Long Y, Xu Y.
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31. Zhang X, Dong Y, Dong H, Zhang W, Li F.
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32. Chen Z, Liu W, Sun X, Zhu L.
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33. Li Q, Shen F, Wang C.
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34. He X, Gao Q, Qiang Y, Guo W, Ma Y.
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35. Liu J, Meng Q, Jing H, Zhou S.
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36. Chen X, Tang L, Feng J, Wang Y, Han Z, Meng J.
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37. Li Z, Pan W, Shen Y, Chen Z, Zhang L, Zhang Y, Luo Q, Ying X
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38. Xu GH, Lou N, Shi HC, Xu YC, Ruan HL, Xiao W, Liu L, Li X, Xiao HB, Qiu B, Bao L, Yuan CF, Zhou YL, Hu WJ, Chen K, Yang HM, Zhang XP.
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39. You S, Guan Y, Li W
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Mol Med Rep. 2018 Jan;17(1):1065-1070. (IF 2.1)

40. Xu GH, Lou N, Shi HC, Xu YC, Ruan HL, Xiao W, Liu L, Li X, Xiao HB, Qiu B, Bao L, Yuan CF, Zhou YL, Hu WJ, Chen K, Yang HM, Zhang XP
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41. Zhang Y, Wang Y, Wang XH, Zhou WJ, Jin LP, Li MQ.
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Mol Med Rep. 2018 Feb;17(2):2869-2878. (IF 2.1)

42. Li S, Wang J, Han Y, Li X, Liu C, Lv Z, Wang X, Tang X, Wang Z.
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Exp Ther Med. 2018 Mar;15(3):2798-2803. (IF 1.785)

43. Yu C, Liu Y, Qin Z.
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44. Sun L, Dong Y, Zhao J, Yin Y, Tong B, Zheng Y, Xin H.
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45. Zhao C, Li Y, Zhang W, Zhao D, Ma L, Ma P, Yang F, Wang Y, Shu Y, Qiu W.
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Int J Oncol. 2018 Mar 7.doi: 10.3892/ijo.2018.4307. (IF 3.899)

46. Zhao C, Li Y, Zhang W, Zhao D, Ma L, Ma P, Yang F, Wang Y, Shu Y, Qiu W
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47. Wang J, Wang C, Bu G.
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48. Chu H, Li H, Guan X, Yan H, Zhang X, Cui X, Li X, Cheng M.
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49. Guo P, Yu C, Wang Q, Zhang R, Meng X, Feng Y
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50. Hairong Chu, Hong Li, Xiumei Guan, Hong Yan, Xiaoyun Zhang, Xiaodong Cui, Xin Li, Min Cheng
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51. Yao Q, Feng M, Yang B, Long Z, Luo S, Luo M, He G, Wang K.
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ACTA BIOCH BIOPH SIN. 2018 May 1;50(5):447-455. (IF 2.836)

52. Wang J, Liang WJ, Min GT, Wang HP, Chen W, Yao N.
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Int J Oncol. 2018 Jun;52(6):1886-1898. (IF 3.899)

53. Wu W, Jingbo S, Xu W, Liu J, Huang Y, Sheng Q, Lv Z
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54. Pan F, Shen F, Yang L, Zhang L, Guo W, Tian J
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55. Tian QT, Ding CY, Song SS, Wang YQ, Zhang A, Miao ZH
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Biochem Pharmacol. 2018 Aug;154:255-264. (IF 4.96)

56. Gao F, Yu X, Meng R, Wang J, Jia L
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57. Ji F, Sha H, Meng F, Zhu A, Ding N, Zhang H, Xu H, Qian H, Yu L, Liu Q, Liu B
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Oncol Rep. 2018 Sep;40(3):1583-1591. (IF 3.417)

58. Dong J, Geng J, Tan W
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Biomed Pharmacother. 2018 Sep;105:922-931. (IF 4.545)

59. Zheng J, Zhang M, Weng H
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BIOCHEM BIOPH RES CO. 2018 Sep 18;503(4):2226-2233. (IF 2.985)

60. Chen LY, Xia XD, Zhao ZW, Gong D, Ma XF, Yu XH, Zhang Q, Wang SQ, Dai XY, Zheng XL, Zhang DW, Yin WD, Tang CK
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61. Cong Bo, Qiang Wu, Hai Zhao, Xuebing Li, Qinghua Zhou
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62. Wang L, Li X, Zhang Y, Huang Y, Zhang Y, Ma Q
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63. Zhang D, Liu H, Luo P, Li Y
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64. Zhou Y, Li H, Xu P, Sun L, Wang Q, Lu Q, Yuan H, Liu Y
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65. Liu W, Zhang L, Wang J, Wang X, Sun H
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66. Cheng D, Zhang X, Tang J, Kong Y, Wang X, Wang S
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67. Zhang C, Wang T, Wu H, Zhang L, Li K, Wang F, Chen Y, Jin J, Hua D
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68. He J, Mu M, Wang H, Ma H, Tang X, Fang Q, Guo S, Song C
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69. Zhang L, Li HX, Pan WS, Khan FU, Qian C, Qi-Li FR, Xu X
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70. Mao J, Yi M, Tao Y, Huang Y, Chen M
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71. Chu HR, Sun YC, Gao Y, Guan XM, Yan H, Cui XD, Zhang XY, Li X, Li H, Cheng M
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72. Wang F, Li Z, Sun B
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73. Wei X, Luo D, Yan Y, Yu H, Sun L, Wang C, Song F, Ge H, Qian H, Li X, Tang X, Liu P
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74. Xie B, Wu J, Li Y, Wu X, Zeng Z, Zhou C, Xu D, Wu L
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75. Wang S, Li J, Zhang C, Xu G, Tang Z, Zhang Z, Liu Y, Wang Z
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76. Cai L, Qi B, Wu X, Peng S, Zhou G, Wei Y, Xu J, Chen S, Liu S
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77. Qin X, Chen Z
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78. Thu PM, Zheng ZG, Zhou YP, Wang YY, Zhang X, Jing D, Cheng HM, Li J, Li P, Xu X
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79. Wang Z, Bi B, Song H, Liu L, Zheng H, Wang S, Shen Z
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80. Man X, Yang L, Liu S, Yang L, Li M, Fu Q
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81. Lin M, Chen L, Xiao Y, Yu B
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82. Zhou H, Li S, Wang G
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83. Li M, Fang XZ, Zheng YF, Xie YB, Ma XD, Liu XT, Xia Y, Shao DH
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84. Liu T, Zou XZ, Huang N, Ge XY, Yao MZ, Liu H, Zhang Z, Hu CP
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85. Wang R, Wang R, Niu X, Cheng Y, Shang X, Li Y, Li S, Liu X, Shao J
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86. Wang F, Ren XF, Chen Z, Li XL, Zhu HJ, Li S, Ou XH, Zhang C, Zhang FX, Zhu BC
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87. Nawab A, Li G, An L, Wu J, Chao L, Xiao M, Zhao Y, Birmani MW, Ghani MW
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88. Wu Y, Xing Y, Zou D
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89. Mu M, Wu F, He J, Tang X, Ma H, Guo S, Song C
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90. Chu J
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91. Bao Z, Fan L, Zhao L, Xu X, Liu Y, Chao H, Liu N, You Y, Liu Y, Wang X, Ji J
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92. Niu G, Yang Y, Ren J, Song T, Hu Z, Chen L, Hong R, Xia J, Ke C, Wang X
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93. Hu Z, Niu G, Ren J, Wang X, Chen L, Hong R, Ke C
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Mol Med Rep. 2019 Nov;20(5):4477-4488. (IF 2.1)

94. Liu Y, Deng X, Wu D, Jin M, Yu B
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95. Sun B, Luo X, Yang C, Liu P, Yang Y, Dong X, Yang Z, Xu J, Zhang Y, Li L
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Stem Cells Int. 2019 Nov 22;2019:8035076. (IF 3.869)

96. Ding He, Hong Chen, Ming Zeng, Chunmei Xia, Jin Wang, Linlin Shen, Danian Zhu, Yonghua Chen, Jijiang Wang
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FRONT NEUROSCI-SWITZ. 2020 Jan 31;14:31.;doi: 10.3389/fnins.2020.00031. (IF 3.707)

97. Qing-Hua Wang, Min Zhang, Ming-Hui Zhou, Xiao-Jiao Gao, Fang Chen, Xun Yan, Feng Lu
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98. Yue Cui, Yuhua Fan, Guangcai Zhao, Qibing Zhang, Ying Bao, Yuanri Cui, Zengjie Ye, Guoyou Chen, Xianji Piao, Fang Guo, Jinghao Wang, Yuhua Bai, Dejun Yu
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99. Lan J, Zhong Z, Wang Y, Xiong Y, Ye Q
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100. Sun J, Lu Y, Yu C, Xu T, Nie G, Miao B, Zhang X
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101. Yingge Liu, Meiying Zhang, Tao He, Weili Yang, Lidong Wang, Lirong Zhang, Mingzhou Guo
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102. Jinjie Sun, Yongtian Lu, Changyun Yu, Ting Xu, Guohui Nie, Beiping Miao, Xin Zhang
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103. Jia’nan Lan, Zibiao Zhong, Yanfeng Wang, Yan Xiong, Qifa Ye
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104. Quan-Xing Shi, Bing Chen, Chuang Nie, Zhi-Ping Zhao, Jun-Hai Zhang, Shao-Yan Si, Shao-Jie Cui, Jian-Wen Gu
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105. Jing-Jing Yang, Xu-Hui Zhang, Xiao-Hui Ma, Wen-Jun Duan, Neng-Gui Xu, Yong-Jun Chen, Lei Liang
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106. Zhu Chen, Jinghua Liu, Lijiao Tian, Qianqian Zhang, Yong Guan, Liang Chen, Gang Liu, Han-Qing Yu, Yangchao Tian, Qing Huang
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109. Jinpeng Lv, Xiaohong An, Songzhou Jiang, Ying Yang, Guoqiang Song, Rongyin Gao
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110. Chengni Jin, Xin Miao, Yujie Zhong, Jiahui Han, Qi Liu, Jiachang Zhu, Xiaodong Xia, Xiaoli Peng
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112. KunBao Yang, YingHui Bai, Ning Yu, BiNan Lu, GuiYan Han, ChangJiang Yin, ZongRan Pang
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113. Qianqian Li, Ningbo Li, Yeting Zeng, Xinrui Wang, Jie Li, Hongying Su, Meiqin Gao, Xiongfei Huang
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115. Bin Li, Jianghong Wu, Jingpiao Bao, Xiao Han, Shuangjun Shen, Xin Ye, Juanjuan Dai, Zengkai Wu, Mengya Niu, Yan He, Jianbo Ni, Li Wen, Xingpeng Wang, Guoyong Hu
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116. Zhaoming Yang, Xiaohang Li, Chengshuo Zhang, Ning Sun, Tingwei Guo, Jianzhen Lin, Feng Li, Jialin Zhang
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117. Qipeng Han, Fangzhu Han, Yisheng Fan, Bowen Lian, Jinyang Xiao, Wei Sun, Dongbing Han, Hongbo Kou, Chunyan Li, Bin Wu
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118. Shibin Feng, Hongyan Ding, Leihong Liu, Chenglu Peng, Yingying Huang, Fuchao Zhong, Wei Li, Tingting Meng, Jinchun Li, Xichun Wang, Yu Li, Jinjie Wu
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119. Guangzhao Song, Liyan Li, Ying Yang
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120. Jian Wen, Yu Jiang, Zhengming Lei, Jingyu He, Mingxin Ye, Wenguang Fu
Leptin Influence Cholelithiasis Formation by Regulating Bile Acid Metabolism
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121. Yu-Long Luo, Sheng Wang, Zhi-Xin Fang, Yi-Chu Nie, Li-Ting Zhang, Chu-Qin Huang, Li Long, Ke-Fang Lai
STAT1 participates in the induction of substance P expression in airway epithelial cells by respiratory syncytial virus
Exp Lung Res. Feb-Mar 2021;47(2):78-86.;doi: 10.1080/01902148.2020.1850922. (IF 1.702)

122. Fan Yang, Yachun Li, Shuai Guo, Yongmei Pan, Cuihuan Yan, Zhiqiang Chen
Hirudo Lyophilized Powder Ameliorates Renal Injury in Diabetic Rats by Suppressing Oxidative Stress and Inflammation
Evid Based Complement Alternat Med. 2021 Feb 22;2021:6657673.;doi: 10.1155/2021/6657673.

123. Mianjie Lin, Ya-Nan Wang, Yixin Ye, Zhelei Xiong, Fengbiao Guo, Haibin Chen
DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells
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124. H-L Li, Y-A Duan, N Zhao
MiR-34a-5p directly targeting TRIM44 affects the biological behavior of ovarian cancer cells
Eur Rev Med Pharmacol Sci. 2021 Feb;25(3):1250-1260.;doi: 10.26355/eurrev_202102_24829.

125. Ling Zhou, Zeming Liu, Sichao Chen, Jing Qiu, Qianqian Li, Shipei Wang, Wei Zhou, Danyang Chen, Guang Yang, Liang Guo
Transcription factor EB‑mediated autophagy promotes dermal fibroblast differentiation and collagen production by regulating endoplasmic reticulum stress and autophagy‑dependent secretion
Int J Mol Med. 2021 Feb;47(2):547-560.;doi: 10.3892/ijmm.2020.4814. (IF 3.098)

126. Huimin Huang, Mingxing Li, Yi Wang, Xiaoxiao Wu, Jing Shen, Zhangang Xiao, Yueshui Zhao, Fukuan Du, Yu Chen, Zhigui Wu, Huijiao Ji, Chunyuan Zhang, Jing Li, Qinglian Wen, Parham Jabbarzadeh Kaboli, Chi Hin Cho, Shengpeng Wang, Yitao Wang, Yisheng He, Xu Wu
Excessive Intake of Longan Arillus Alters gut Homeostasis and Aggravates Colitis in Mice
Front Pharmacol. 2021 Mar 26;12:640417.;doi: 10.3389/fphar.2021.640417. (IF 4.225)

127. Binzhen Chen, Jia Han, Shaoheng Chen, Rufeng Xie, Jie Yang, Tongming Zhou, Qi Zhang, Rong Xia
MicroLet-7b Regulates Neutrophil Function and Dampens Neutrophilic Inflammation by Suppressing the Canonical TLR4/NF-κB Pathway
Front Immunol. 2021 Mar 29;12:653344.;doi: 10.3389/fimmu.2021.653344. (IF 5.085)

128. Cheng Gao, Jianbo Shen, Lanqing Yao, Zhenguo Xia, Xiaoliang Liang, Renfei Zhu, Zhong Chen
Low expression of AQP9 and its value in hepatocellular carcinoma
Transl Cancer Res. 2021 Apr;10(4):1826-1841.;doi: 10.21037/tcr-20-3158. (IF 0.986)

129. Liu Li, Li Yang, Feng Yang, Xin-Lan Zhao, Shengjiang Xue, Fang-Hua Gong
Ginkgo biloba Extract 50 (GBE50) Ameliorates Insulin Resistance, Hepatic Steatosis and Liver Injury in High Fat Diet-Fed Mice
J Inflamm Res. 2021 May 17;14:1959-1971.;doi: 10.2147/JIR.S302934.

130. Sijia Lei, Bin Zhang, Luyuan Huang, Ziyou Zheng, Shaohan Xie, Lianghua Shen, Mason Breitzig, Alexander Czachor, Hongtao Liu, Huiru Luo, Yanxia Chen, Kangshou Liu, Hanxiao Sun, Qing Zheng, Qiang Li, Feng Wang
SRSF1 promotes the inclusion of exon 3 of SRA1 and the invasion of hepatocellular carcinoma cells by interacting with exon 3 of SRA1pre-mRNA
Cell Death Discov. 2021 May 19;7(1):117.;doi: 10.1038/s41420-021-00498-w.

131. Ming Qin, Wei Chen, Zhixin Li, Lixue Wang, Lixia Ma, Jinhong Geng, Yu Zhang, Jing Zhao, Yongqing Zeng
Role of IFNLR1 gene in PRRSV infection of PAM cells
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132. Chen Zhang, Min Chen, Zhangcai Chi
Cytokine secretion and pyroptosis of cholesteatoma keratinocytes mediated by AIM2 inflammasomes in response to cytoplasmic DNA
Mol Med Rep. 2021 May;23(5):344.;doi: 10.3892/mmr.2021.11983. (IF 2.1)

133. Xu Huang, Nan Bi, Jingbo Wang, Hua Ren, Desi Pan, Xianping Lu, Luhua Wang
Chidamide and Radiotherapy Synergistically Induce Cell Apoptosis and Suppress Tumor Growth and Cancer Stemness by Regulating the MiR-375-EIF4G3 Axis in Lung Squamous Cell Carcinomas
J Oncol. 2021 Jun 11;2021:4936207.;doi: 10.1155/2021/4936207. (IF 2.206)

134. Xiaohui Xu, Yong Wu, Ke Yi, Yan Hu, Weiqun Ding, Chungen Xing
IRF1 regulates the progression of colorectal cancer via interferon‑induced proteins
Int J Mol Med. 2021 Jun;47(6):104.;doi: 10.3892/ijmm.2021.4937. (IF 3.098)

135. Yu Jia, Hui-Yan Li, Ying Wang, Jue Wang, Jing-Wen Zhu, Yan-Yan Wei, Lu Lou, Xing Chen, Shi-Jing Mo
Crosstalk between hypoxia-sensing ULK1/2 and YAP-driven glycolysis fuels pancreatic ductal adenocarcinoma development
Int J Biol Sci. 2021 Jul 5;17(11):2772-2794.;doi: 10.7150/ijbs.60018. (IF 4.858)

136. Fengshuo Zhu, Xiaoguang Li, Xiao Tang, Junjian Jiang, Yu Han, Yinuo Li, Chunyue Ma, Zhonglong Liu, Yue He
Neferine promotes the apoptosis of HNSCC through the accumulation of p62/SQSTM1 caused by autophagic flux inhibition
Int J Mol Med. 2021 Jul;48(1):124.;doi: 10.3892/ijmm.2021.4957. (IF 3.098)

137. Qingping Song, Hui Liu, Chengyan Li, Haifeng Liang
miR-33a-5p inhibits the progression of esophageal cancer through the DKK1-mediated Wnt/β-catenin pathway
Aging (Albany NY). 2021 Aug 23;13(16):20481-20494.;doi: 10.18632/aging.203430.

138. Cheng Gao, Jianbo Shen, Weipeng Chen, Lanqing Yao, Xiaoliang Liang, Renfei Zhu, Zhong Chen
Increased RBM12 expression predicts poor prognosis in hepatocellular carcinoma based on bioinformatics
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139. Yuxin Wang, Yixun Su, Guangdan Yu, Xiaorui Wang, Xiaoying Chen, Bin Yu, Yijun Cheng, Rui Li, Juan C Sáez, Chenju Yi, Lan Xiao, Jianqin Niu
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140. Xiao-Yue Ge, Tian-Tian Zhu, Mao-Zhong Yao, Hong Liu, Qian Wu, Jie Qiao, Wei-Fang Zhang, Chang-Ping Hu
MicroRNA-137 Inhibited Hypoxia-Induced Proliferation of Pulmonary Artery Smooth Muscle Cells by Targeting Calpain-2
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141. Huan Lan, Lu-Ying Zhang, Wen He, Wan-Ying Li, Zhen Zeng, Bo Qian, Chengqiang Wang, Jia-Le Song
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Bio-Serv动物模型饲料K3588   Mouse Fort, Red

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Bio-Serv动物模型饲料K3588   Mouse Fort, Red

K3588

Product Details
  • Certified (Contaminant Screened)
  • Made of high temp polycarbonate
  • Transparent material provides ease of visibility
  • Provides shelter and a secure place to build a nest
  • Low profile fits in most shoebox cages
  • Angled sides prevent pushing against the water source
  • Flat top gives additional floor space
  • Autoclavable
  • Cagewasher safe
Dimensions
5 1/4″ Length x 5 1/4″ wide x 1 3/4″ tall
with 4 openings
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His Tag Mouse mAb (HRP Conjugated)(His Tag 小鼠单抗)(AF2879-1ml)

His Tag Mouse Monoclonal Antibody (HRP Conjugated)
His Tag Mouse mAb (HRP Conjugated)(His Tag 小鼠单抗)(AF2879-1ml) 一键复制产品信息

产品编号: AF2879-1ml

产品包装:1ml
选择包装

50μl 200μl 1ml

说明书下载

4586.00 10

价格: ¥ 4586.00

促销价: ¥ 4586.00

促销价: ¥ 4586.00

产品简介
使用说明
产品文件
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产品编号 产品名称 产品包装 产品价格
AF2879-50μl His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl 388.00元
AF2879-200μl His Tag Mouse Monoclonal Antibody (HRP Conjugated) 200μl 1168.00元
AF2879-1ml His Tag Mouse Monoclonal Antibody (HRP Conjugated) 1ml 4586.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Mouse WB, IHC, ICC His-tagged protein in all species

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:500-1:2000 1:50-1:100 1:50-1:100

抗体详细信息如下:

About this Antibody
Name His Tag Mouse Monoclonal Antibody (HRP Conjugated)
Category Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody
Isotype IgG1
Purificationmethod Affinity purification
Positive samples
Cellular location
Customer validation
About the Immunogen
Immunogen Synthetic His tag peptide coupled to KLH
Sequence HHHHHH
Gene ID
SwissProt
Synonyms HHHHHH
Category Polypeptide protein tag
Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. A variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography (1). As is the case with other protein tag systems (2), this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest.

包装清单:

产品编号 产品名称 包装
AF2879-50µl His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份

产品编号 产品名称 包装
AF2879-200µl His Tag Mouse Monoclonal Antibody (HRP Conjugated) 200µl
AZ200 Western一抗稀释液 200ml
说明书 1份

产品编号 产品名称 包装
AF2879-1ml His Tag Mouse Monoclonal Antibody (HRP Conjugated) 1ml
AZ502 Western一抗稀释液 AZ500×2
说明书 1份

保存条件:

His Tag Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

请根据抗体的实际用途选择相应的使用方法。
1.Western检测:
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗,4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作,无须再孵育二抗。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。

相关产品:

产品编号 产品名称 包装
AF2852 Flag Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2855 Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2858 HA Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2861 HA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2864 Myc Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2867 Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2870 His Tag (C-terminal Specific) Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2873 His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2876 His Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2879 His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2882 GFP Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2885 GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2888 GST Tag Mouse Monoclonal Antibody 50μl/200μl
AF2891 GST Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2894 V5 Tag Mouse Monoclonal Antibody 50μl/200μl
AF2897 V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2900 Glu-Glu Tag Mouse Monoclonal Antibody 50μl
AF2906 DsRed Tag Mouse Monoclonal Antibody 50μl
AF2909 DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2912 MBP Tag Mouse Monoclonal Antibody 50μl
AF2915 MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2918 PA Tag Mouse Monoclonal Antibody 50μl
AF2921 PA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2924 StrepII Tag Mouse Monoclonal Antibody 50μl
AF2927 StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2930 S Tag Mouse Monoclonal Antibody 50μl
AF2933 S Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2936 TEV-His Tag Mouse Monoclonal Antibody 50μl
AF2939 TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2942 Target Tag Mouse Monoclonal Antibody 50μl
AF2945 Target Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2948 TRX Tag Mouse Monoclonal Antibody 50μl
AF2951 TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2954 AVI Tag Mouse Monoclonal Antibody 50μl
AF2957 AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl

His Tag Mouse mAb (HRP Conjugated)(His Tag 小鼠单抗)(AF2879-1ml)
His Tag Mouse mAb (HRP Conjugated)(His Tag 小鼠单抗)(AF2879-1ml)

物质安全数据单
输入右侧验证码点击下载:

His Tag Mouse mAb (HRP Conjugated)(His Tag 小鼠单抗)(AF2879-1ml)

His Tag Mouse mAb (HRP Conjugated)(His Tag 小鼠单抗)(AF2879-1ml)
质检证书
输入批号搜寻COA

搜索

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HRP标记一抗

使用本产品的文献:

重组小鼠EPHA2蛋白(His标签) Mouse EPHA2 Protein

重组小鼠EPHA2蛋白(His标签) Mouse EPHA2 Protein

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

Epithelial cell kinase;Ephrin type-A receptor 2; ECK

表达区间及表达系统(Source)

Mouse EPHA2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys26-Val538.[Accession | Q03145]

分子量大小(Molecular Weight)

The protein has a predicted MW of 57.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

Epithelial cell kinase;Ephrin type-A receptor 2; ECK

表达区间及表达系统(Source)

Mouse EPHA2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys26-Val538.[Accession | Q03145]

分子量大小(Molecular Weight)

The protein has a predicted MW of 57.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

His Tag Mouse mAb(His Tag 小鼠单抗)(AF2876-1ml)

His Tag Mouse Monoclonal Antibody
His Tag Mouse mAb(His Tag 小鼠单抗)(AF2876-1ml) 一键复制产品信息

产品编号: AF2876-1ml

产品包装:1ml
选择包装

50μl 200μl 1ml

说明书下载

3528.00 10

价格: ¥ 3528.00

促销价: ¥ 3528.00

促销价: ¥ 3528.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
AF2876-50μl His Tag Mouse Monoclonal Antibody 50μl 299.00元
AF2876-200μl His Tag Mouse Monoclonal Antibody 200μl 899.00元
AF2876-1ml His Tag Mouse Monoclonal Antibody 1ml 3528.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Mouse WB, IP, IF, IHC, ICC His-tagged protein in all species

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:500-1:2000 1:50 1:50-1:100 1:50-1:100 1:50-1:100

抗体详细信息如下:

About this Antibody
Name His Tag Mouse Monoclonal Antibody
Category Mouse Monoclonal Antibody (mAb); Primary antibody
Isotype IgG1
Purificationmethod Affinity purification
Positive samples
Cellular location
Customer validation
About the Immunogen
Immunogen Synthetic His tag peptide coupled to KLH
Sequence HHHHHH
Gene ID
SwissProt
Synonyms HHHHHH
Category Polypeptide protein tag
Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. A variety of plasmids contain DNA that encodes an amino-terminal tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography (1). As is the case with other protein tag systems (2), this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest.

包装清单:

产品编号 产品名称 包装
AF2876-50µl His Tag Mouse Monoclonal Antibody 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份

产品编号 产品名称 包装
AF2876-200µl His Tag Mouse Monoclonal Antibody 200µl
AZ200 Western一抗稀释液 200ml
说明书 1份

产品编号 产品名称 包装
AF2876-1ml His Tag Mouse Monoclonal Antibody 1ml
AZ502 Western一抗稀释液 AZ500×2
说明书 1份

保存条件:

His Tag Mouse Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

请根据抗体的实际用途选择相应的使用方法。
1. Western检测:
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品:

产品编号 产品名称 包装
AF2852 Flag Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2855 Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2858 HA Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2861 HA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2864 Myc Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2867 Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2870 His Tag (C-terminal Specific) Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2873 His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2876 His Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2879 His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2882 GFP Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2885 GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2888 GST Tag Mouse Monoclonal Antibody 50μl/200μl
AF2891 GST Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2894 V5 Tag Mouse Monoclonal Antibody 50μl/200μl
AF2897 V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2900 Glu-Glu Tag Mouse Monoclonal Antibody 50μl
AF2906 DsRed Tag Mouse Monoclonal Antibody 50μl
AF2909 DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2912 MBP Tag Mouse Monoclonal Antibody 50μl
AF2915 MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2918 PA Tag Mouse Monoclonal Antibody 50μl
AF2921 PA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2924 StrepII Tag Mouse Monoclonal Antibody 50μl
AF2927 StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2930 S Tag Mouse Monoclonal Antibody 50μl
AF2933 S Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2936 TEV-His Tag Mouse Monoclonal Antibody 50μl
AF2939 TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2942 Target Tag Mouse Monoclonal Antibody 50μl
AF2945 Target Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2948 TRX Tag Mouse Monoclonal Antibody 50μl
AF2951 TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2954 AVI Tag Mouse Monoclonal Antibody 50μl
AF2957 AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl

His Tag Mouse mAb(His Tag 小鼠单抗)(AF2876-1ml)
His Tag Mouse mAb(His Tag 小鼠单抗)(AF2876-1ml)

物质安全数据单
输入右侧验证码点击下载:

His Tag Mouse mAb(His Tag 小鼠单抗)(AF2876-1ml)

His Tag Mouse mAb(His Tag 小鼠单抗)(AF2876-1ml)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
标签抗体

使用本产品的文献:

Mouse Noggin 重组小鼠Noggin(小鼠Noggin)

Mouse Noggin 重组小鼠Noggin(小鼠Noggin)

产品说明书

FAQ

COA

已发表文献

Noggin is a bone morphogenetic protein (BMP) antagonist expressed in Spemann's organizer.  It inhibits TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin was originally found as a BMP-4 antagonist, and then has been shown to modulate the activities of other BMPs (BMP-2, 7, 13 and 14). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Secreted noggin protein regulates bone morphogenetic protein activity during development. The Role of the BMP Signaling Antagonist Noggin in the Development of Prostate Cancer Osteolytic Bone Metastasis. Lack of expression of the BMP antagonist noggin by osteoinductive, carcinoma-derived cell lines is a determinant of the osteoblast response induced by their bone metastases. In contrast, osteolytic, carcinoma-derived cell lines express noggin constitutively.

 

Product Properties

Synonyms

NOG, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A

Accession

P97466

GeneID

18121

Source

E.coli-derived human Noggin protein, Gln28-Cys232, with an N-terminal Met.

Molecular Weight

Approximately 46.4 kDa.

AA Sequence

MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0 × 105 IU/mg in the presence of 5 ng/mL BMP-4. Fully biologically active when compared to standard.

Endotoxin

1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration less than 0.25 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1.Avoid repeated freeze-thaw cycles.

2.For your safety and health, please wear lab coats and disposable gloves for operation.

3.For research use only!

HB221020

Mouse Noggin 重组小鼠Noggin(小鼠Noggin)

暂无内容

Mouse Noggin 重组小鼠Noggin(小鼠Noggin)

暂无内容

Noggin is a bone morphogenetic protein (BMP) antagonist expressed in Spemann's organizer.  It inhibits TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin was originally found as a BMP-4 antagonist, and then has been shown to modulate the activities of other BMPs (BMP-2, 7, 13 and 14). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Secreted noggin protein regulates bone morphogenetic protein activity during development. The Role of the BMP Signaling Antagonist Noggin in the Development of Prostate Cancer Osteolytic Bone Metastasis. Lack of expression of the BMP antagonist noggin by osteoinductive, carcinoma-derived cell lines is a determinant of the osteoblast response induced by their bone metastases. In contrast, osteolytic, carcinoma-derived cell lines express noggin constitutively.

 

Product Properties

Synonyms

NOG, SYM1, symphalangism 1 (proximal), synostoses (multiple) syndrome 1, SYNS1, SYNS1A

Accession

P97466

GeneID

18121

Source

E.coli-derived human Noggin protein, Gln28-Cys232, with an N-terminal Met.

Molecular Weight

Approximately 46.4 kDa.

AA Sequence

MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0 × 105 IU/mg in the presence of 5 ng/mL BMP-4. Fully biologically active when compared to standard.

Endotoxin

1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration less than 0.25 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1.Avoid repeated freeze-thaw cycles.

2.For your safety and health, please wear lab coats and disposable gloves for operation.

3.For research use only!

HB221020

Mouse Noggin 重组小鼠Noggin(小鼠Noggin)

暂无内容

Mouse Noggin 重组小鼠Noggin(小鼠Noggin)

暂无内容

S Tag Mouse mAb (HRP Conjugated)(S Tag 小鼠单抗)(AF2933)

S Tag Mouse Monoclonal Antibody (HRP Conjugated)
S Tag Mouse mAb (HRP Conjugated)(S Tag 小鼠单抗)(AF2933) 一键复制产品信息

产品编号: AF2933

产品包装:50μl
选择包装

50μl

说明书下载

388.00 10

价格: ¥ 388.00

促销价: ¥ 388.00

促销价: ¥ 388.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
AF2933 S Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl 388.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Mouse WB, IHC, ICC S-tagged protein in all species

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:500-1:2000 1:50-1:100 1:50-1:100

抗体详细信息如下:

About this Antibody
Name S Tag Mouse Monoclonal Antibody (HRP Conjugated)
Category Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody
Isotype IgG1
Purificationmethod Affinity purification
Positive samples
Cellular location
Customer validation
About the Immunogen
Immunogen Synthetic S tag peptide coupled to KLH
Sequence KETAAAKFERQHMDS
Gene ID
SwissProt
Synonyms S-protein
Category Polypeptide protein tag
Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. S-Tag is an epitope tag composed of a 15 residue peptide (KETAAAKFERQHMDS) that, together with S-protein, comprises pancreatic ribonuclease S.

包装清单:

产品编号 产品名称 包装
AF2933 S Tag Mouse Monoclonal Antibody (HRP Conjugated) 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份

保存条件:

S Tag Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

请根据抗体的实际用途选择相应的使用方法。
1.Western检测:
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗,4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作,无须再孵育二抗。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。

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S Tag Mouse mAb (HRP Conjugated)(S Tag 小鼠单抗)(AF2933)
S Tag Mouse mAb (HRP Conjugated)(S Tag 小鼠单抗)(AF2933)

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S Tag Mouse mAb (HRP Conjugated)(S Tag 小鼠单抗)(AF2933)

S Tag Mouse mAb (HRP Conjugated)(S Tag 小鼠单抗)(AF2933)
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