标签归档：methylcytidine

5-Methylcytidine抗体现货 BI-MECY-0100

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Anti-5-Methylcytidine, mAb
Eurogentec公司的5-Methylcytidine抗体现货供应

AnaSpec, Eurogentec’s newest North American division, is pleased to offer Eurogentec’s popular monoclonal antibody, anti-5-methylcytidine （Clone 33D3） for use in epigenetics research. Purified from ascites, this IgG1 / λ isotype antibody is supplied as a 1 mg/ml solution （in PBS） and is available in three sizes （0.1 mg, 0.5 mg and 1 mg）.

5-Methylcytidine is a modified base found in the DNA of plants and vertebrate. DNA methylation is a post-replication process involved in the establishment of genomic imprinting, in the control of gene expression and of differentiation. Alterations of DNA methylation pattern are associated with carcinogenesis. A global DNA hypomethylation is often detected in tumor tissues, associated with local hypermethylation sites. Since this antibody discriminates between the modified base, 5-MeCyd and its normal counterpart, cytidine; it is a useful tool for gene promoter methylation analysis.

The most published anti 5-Methylcytidine antibody available on the market, anti-5-Methylcyidine from Clone 33D3 can be used in applications such as Methylated DNA Immunoprecipitation （MeDIP）, MeDIP-on-chip assay, immunofluorescence, flow cytometry and immunohistochemistry. Histopathological analysis of tissue samples can be performed without having to destroy cells through DNA extraction procedures.

This antibody has been used to detect alterations in the urinary excretion of nucleosides from cancer patients, to visualize the distribution of methyl-rich regions along human chromosomes, to quantify in situ differences between normal and malignant cells from peripheral blood as well as on tissue sections.

Product

Size

Catalog #

Monoclonal Antibody against 5-Methylcytidine NEW
Host and Clonality: Mouse monoclonal; Application: ELISA, WB, FC, ICC, IHC, and Cytogenetics

0.1 mg

BI-MECY-0100

Monoclonal Antibody against 5-Methylcytidine NEW
Host and Clonality: Mouse monoclonal; Application: ELISA, WB, FC, ICC, IHC, and Cytogenetics

0.5 mg

BI-MECY-0500

Monoclonal Antibody against 5-Methylcytidine NEW
Host and Clonality: Mouse monoclonal; Application: ELISA, WB, FC, ICC, IHC, and Cytogenetics

1 mg

BI-MECY-1000

The following papers are examples of recent publications citing the use of this antibody. To view a citation archive, please click here.

Jin, SG. et al. （2010）. Examination of the specificity of DNA methylation profiling techniques towards 5-methylcytosine and 5-hydroxymethylcytosine. Nucleic Acid Res. doi:10.1093/nar/gkq22.

Sanchez-Abarca, LI. et al. （2010）. Immunomodulatory effect of 5-azacytidine （5-azaC）: potential role in the transplantation setting. Blood 115: 107, doi: 10.1182/blood-2009-03-210393.

Wolk, M. & JE. Martin （2009）. Titrimetric immunohistochemical evaluation of DNA hypomethylation in uterine tumours. J. Clin. Pathol. 62:1039, doi:10.1136/jcp.2009.066613.

Peters, DD. et al. （2009）. Mammalian Genome 20: 664, doi: 10.1007/s00335-009-9227-0.

Mutskov, V. & G. Felsenfeld （2009）. The human insulin gene is part of a large open chromatin domain specific for human islets. Proc. Natl. Acad. Sci. 106: 17419, doi: 10.1073/pnas.0909288106.

Other epigenetics products include a wide selection of histone peptides, especially histone H3 peptides, SensoLyte® HDAC, LSD1 and SIRT1 assay kits.

 

 

5-Methylcytidine-5′-Triphosphate

发表于由

5-Methylcytidine-5′-Triphosphate

简要描述：5-甲基胞苷-5-三磷酸盐是TRILINK公司产品，用于干细胞研究用

详细介绍

产品咨询

5-甲基胞苷-5-三磷酸盐是TRILINK公司产品，用于干细胞研究用,5-methyl-ctp

Synonyms]
5-methyl-dCTP
5-methyldeoxycytidine triphosphate
CPD-1094
5-methyl deoxycytidine-5′-triphosphate
5-methyl-2′-deoxycytidine-5′-triphosphate
cytidine 5′-（tetrahydrogen triphosphate）, 2′-deoxy-5-methyl-
[[[5-[（4-amino-5-methyl-2-oxo-1H-pyrimidin-1-yl）]-3-hydroxy-tetrahydrofuran-2-yl]methoxy-hydroxy-phosphinoyl]oxy-hydroxy-phosphinoyl]oxyphosphonic acid

[Structure]
 

[ Properties Computed from Structure]
 

Molecular Weight 481.183503 [g/mol]
Molecular Formula C10H18N3O13P3
XLogP -5.9
H-Bond Donor 6
H-Bond Acceptor 14
Rotatable Bond Count 8
Tautomer Count 3
Exact Mass 481.005247
MonoIsotopic Mass 481.005247
Topological Polar Surface Area 248
Heavy Atom Count 29
Formal Charge 0
Complexity 868
Isotope Atom Count 0
Defined Atom StereoCenter Count 0
Undefined Atom StereoCenter Count 3
Defined Bond StereoCenter Count 0
Undefined Bond StereoCenter Count 0
Covalently-Bonded Unit Count 1
Description
5-Methyl-dCTP is widely used for construction of cDNA libraries
 		 Incorporation of 5-Methyl-dCTP
M-MuLV Reverse Transcriptase
Klenow Fragment of DNA Polymerase I
Sequenase DNA Polymerase
（Taq Polymerase, Vent） *
Incorporation of Hg-dCTP
DNA Polymerase I

References to 5-Methyl-dCTP
Lefaucheur et al. （1998） Evidence for three adult fast myosin heavy chain isoforms in type II skeletal muscle fibers in pigs. J. Anim. Sci. 76:1584.
Nelson et al. （1993） Restriction endonuclease cleavage of 5-methyl-deoxycytosine hemimethylated DNA at high enzyme-to-substrate ratios. Nucl. Acids Res. 21 （3）:681.
Asamizu et al. （1999） A large scale structural analysis of cDNAs in a unicellular green alga, Chlamydomonas reinhardtii. I. Generation of 3433 non-redundant expressed sequence tags. DNA Research 6:369.
* Wong et al. （1991） PCR with 5-methyl-dCTP replacing dCTP. Nucl. Acids Res. 19 （5）:1081.
Reference to Hg-dCTP
Banfalvi et al. （1995） Effect of mercury substitution of DNA on its susceptibility to cleavage by restriction endonucleases. DNA Cell Biol. 14 （5）:445.
 

 

N-1014-1 5-Methylcytidine-5′-TP 1umole 1317.5
N-1014-10 5-Methylcytidine-5′-TP 10umoles 10625
N-1014-5 5-Methylcytidine-5′-TP 5umoles 6205

 

Reference（s）
Kariko K, Muramatsu H, Ludwig J, Weissman D. Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein encoding mRNA. （2011） Nucleic Acids Research.
         
Kormann M, Hasenpusch G, Aneja M, et al. Expression of therapeutic proteins after delivery of chemically modified mRNA in mice. （2011） Nature Biotechnology 29:154–157.
         
Anderson, B., Muramatsu, H., Nallagatla, S.R., Bevilacqua, P.C., Sansing, L.H., Weissman, D. & Kariko, K. Incorporation of pseudouridine into mRNA enhances translation by dimishing PKR activation （2010） Nucleic Acids Research, 38（17）: 5884-5892.
         
Warren et al., Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA, Cell Stem Cell （2010）, doi:10.1016/j.stem.2010.08.012.
         
Kariko K, Muramatsu H, Welsh F, et al. Incorporation of Pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. （2008） Molecular Therapy （16）11: 1833-1840.
         
Kariko, K., Buckstein, M., Ni, H. & Weissman, D. Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modifiation and the Evolutionary Origin of RNA （2005）. Immunity, 23（2）, 165-175.
         
Lefmann M, et al. Novel Mass Spectrometry-based tool for genotypic identification of mycobacteria. （2004） Journal of Clinical Microbiology, 42（1）: 339-346.
         
Hartmer R, Storm N, Boecker S, Rodi CP, Hillenkamp F, Jurinke C, van den Boom D. RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis. （2003） Nucleic Acids Res., 31（9）: e47.
         
Nguyen A, Zhao C, Dorris D, Mazumder A. Quantitative assessment of the use of modified nucleoside triphosphates in expression profiling: differential effects on signal intensities and impacts on expression ratios. （2002） BMC Biotechnology, 2（1）: 14.
         
Van Rompay AR, Norda A, Linden K, Johansson M, Karlsson A. Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases. （2001） Mol Pharmacol., 59（5）: 1181-6.