MS、B5、N6常用培养基选购指南 |
MS(Murashige & Skoog)培养基是目前普遍使用的培养基。它有较高的无机盐浓度,对保证组织生长所需的矿质营养和加速愈伤组织的生长十分有利。由于配方中的离子浓度高,在配制、贮存、消毒等过程中,即使有些成分略有出入,也不致影响离子间的平衡。MS固体培养基可用来诱导愈伤组织,或用于胚、茎段、茎尖及花药培养,它的液体培养基用于细胞悬浮培养时能获得明显成功。这种培养基中的无机养分的数量和比例比较合适,足以满足植物细胞在营养上和生理上的需要。因此,一般情况下,无须再添加氨基酸、酪蛋白水解物、酵母 提取物及椰子汁等有机附加成分。与其它培养基的基本成分相比,MS培养基中的硝酸盐、钾和铵的含量高,这是它的明显特点。PhytoTech提供多种MS 培养基供您选择。 |
货号 |
名称 |
M519 |
Murashige & Skoog (MS) Basal Medium w/Vitamins |
MS基本培养基 含维生素 |
M524 |
Murashige & Skoog (MS) Basal Salt Mixture |
MS基本培养基 不含维生素 |
M153 |
Murashige & Skoog (MS) Modified Basal Salt Mixture |
1/2MS培养基 |
M407 |
Murashige & Skoog (MS) Modified Basal Salt Mixture without Nitrogen, Phosphorous, & Potassium |
MS基本培养基 不含氮磷钾 |
M531 |
Murashige & Skoog Modified Basal Salt Mixture (Without Nitrogen) |
MS基本培养基 不含氮 |
M561 |
Murashige & Skoog Modified Basal Salt Mixture (1?2x Nitrates) |
MS基本培养基 1/2硝酸盐 |
M571 |
Murashige & Skoog Modified Basal Salt Mixture (Without NH4NO3) |
MS基本培养基 |
M290 |
Murashige & Skoog Modified Basal Salt Mixture (1?2x Nitrates & 1?2x Calcium) |
MS 基本培养基 含1/2硝酸盐与1/2钙盐 |
M404 |
Murashige & Skoog (MS) Basal Medium w/ Gamborg Vitamins |
MS 基本培养基与 B5 维生素混合物 |
M533 |
M & S Vitamin Powder (1000x) |
MS基本培养基中的维生素粉末1000x |
M553 |
M & S Vitamin Solution (1000x) |
MS基本培养基中的维生素溶液1000x |
M547 |
M & S Modified Vitamin Powder(1000x) |
MS改良培养基中的维生素粉末1000x,含硫胺素 |
M557 |
M & S MOD VIT Solution (1000x) |
MS改良培养基中的维生素溶液1000x, 含硫胺素 |
M530 |
M & S Modified Basal Medium |
MS改良培养基 含MES缓冲液 |
M502 |
Murashige & Skoog Macronutrient Salt Base |
MS大量元素 |
M654 |
M & S Macronutrient Stock SOL(10x) |
MS大量元素10x储存液 |
M554 |
Murashige & Skoog Micronutrient Salt Base |
MS微量元素 |
M529 |
M & S Micronutrient Stock SOL(10x) |
MS微量元素10x储存液 |
M516 |
Murashige & Skoog Modified BC Potato Basal Medium |
MS基本培养基 含激动素 |
M401 |
Murashige & Skoog (MS) Modified Basal Medium |
MS基本培养基 含1.0 BA &0.1 NAA,30g/L的蔗糖(mg/L) |
M5642 |
Murashige & Skoog (MS) Basal Salt Mixture Van der Salm Modification |
MS基本培养基 含维生素,其中铁源由FeNa-EDDHA 代替硫酸亚铁和Na2EDTA?2H2O |
M5541 |
Murashige & Skoog (MS) Basal Salt Mixture Van der Salm Modification |
MS基本培养基,其中铁源由FeNa-EDDHA 代替硫酸亚铁和Na2EDTA?2H2O |
M550 |
Murashige & Skoog (MS)Modified Medium Arabidopsis Culture Medium |
MS基本培养基含改良的维生素,同时含2.0 2,4-D, 0.05激动素和蔗糖 |
M701 |
Murashige & Skoog (MS)Modified Basal Medium |
MS基本培养基,其中铁源由FeNa-EDTA 代替Na2EDTA?2H2O, 10.0 2iP, 1.0 IAA, and 0.4 硫胺素代替MS维生素(mg/L) |
M702 |
Murashige & Skoog (MS)Modified Basal Medium |
MS基本培养基,80 腺嘌呤硫酸盐, 148磷酸钠, 30.0 2iP, 0.3 IAA, 30,000 蔗糖 and 0.4硫胺素代替MS维生素)(mg/L) |
M419 |
MG Basal salt mixture |
改良的MS和B5的基盐混合物 |
M504 |
MS Basal salt mixture, FINER & NAGASAWA Modification |
MS基盐,FINER&NAGASAWA 改良 |
M5615 |
MS Liquid medium modified to contain 15g/L sucrose and 15g/L glucose |
滤灭的MS改良液体培养基含15g/L的蔗糖和15g/L葡萄糖 |
M5530 |
MS Liquid medium modified to contain 30g/L sucrose |
滤灭的MS改良液体培养基含30g/L的蔗糖 |
M5501 |
MS medium w/ vitamins & sucrose |
MS培养基含有30g/L的蔗糖 |
M5825 |
MS medium w/ vitamins ,sucrose & Gelzan |
MS培养基含有30g/L的蔗糖和2g/L的琼脂(G3251) |
M5707 |
MS Medium w/ Vitamins, Sucrose, and Glucose |
MS培养基含有10g/L的蔗糖和20g/L葡萄糖 |
M576 |
MURASHIGE & SKOOG (MS) BASAL SALT CONCENTRATE (20X) |
MS液体培养基基盐 20× |
M541 |
MURASHIGE & SKOOG (MS) MODIFIED BASAL MEDIUM |
MS改良培养基不含 KH2PO4,含300mg/L磷酸钠,150mg/L硫酸腺嘌呤和1000mg/L水解酪蛋白 |
M555 |
Murashige & Skoog Modified Multiplication Medium |
MS基盐 LS维生素 148 磷酸二氢钠 80 硫酸腺嘌呤1.0 Kinetin, 0.1 NAA, and 30,000 蔗糖(mg/L) |
M5531 |
Murashige & Skoog Medium w/ 1g/L MES |
在M519的基础上加入1g/L MES |
M499 |
MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE W/ FeNaEDTA |
MS基盐的基础上用FeNaEDTA代替了硫酸亚铁和NaEDTA |
M517 |
Murashige Modified African Violet/Gloxinia Multiplication Basal Medium |
MS基盐 LS维生素 170 磷酸二氢钠 2.0 IAA, 2.0 Kinetin,其中铁源由FeNa-EDDHA 代替Na2EDTA?2H2O(mg/L) |
M518 |
Murashige Modified African Violet/Gloxinia Pretransplant Basal Medium |
MS基盐 LS维生素 1.0 IAA 其中铁源由FeNa-EDDHA 代替硫酸亚铁和Na2EDTA?2H2O(mg/L) |
M508 |
Murashige Modified Fern Multiplication Basal Medium |
MS基盐 LS维生素 255磷酸二氢钠2.0 Kinetin, 1.0 IAA 其中铁源由FeNa-EDDHA 代替硫酸亚铁和Na2EDTA?2H2O(mg/L) |
M509 |
Murashige Modified Gerbera Multiplication Basal Medium |
MS基盐和改良维生素,85磷酸二氢钠, 80 硫酸腺嘌呤, 10.0 Kinetin, 0.5 IAA, 100 L-酪氨酸(mg/L)其中铁源由FeNa-EDDHA 代替硫酸亚铁和Na2EDTA?2H2O |
M510 |
Murashige Modified Gerbera Pretransplant Basal Medium |
MS基盐和改良维生素,85磷酸二氢钠, 10.0 IAA, 100 L-酪氨酸 其中铁源由FeNa-EDDHA 代替Na2EDTA?2H2O(mg/L) |
M511 |
Murashige Modified Kalanchoe Multiplication Basal Medium |
MS基盐 LS维生素,3.0 2iP,其中铁源由FeNa-EDDHA 代替硫酸亚铁和Na2EDTA?2H2O(mg/L) |
M512 |
MURASHIGE KALANCHOE PRETRANSPLANT MEDIUM |
MS基盐 LS维生素,3.0 IAA,其中铁源由FeNa-EDDHA 代替硫酸亚铁和Na2EDTA?2H2O(mg/L) |
M536 |
Murashige Modified Multiplication Basal Medium |
MS培养基,含170 磷酸二氢钠, 80硫酸腺嘌呤, 0.4 硫胺(mg/L) |
M527 |
MURASHIGE MODIFIED MULTIPLICATION MEDIUM |
MS基盐 LS维生素,1.0 KT 0.3 IAA,其中铁源由FeNa-EDDHA 代替硫酸亚铁和Na2EDTA?2H2O(mg/L) |
M491 |
MURASHIGE SHOOT MULTIPLICATION MEDIUM |
MS基盐 LS维生素,170 磷酸二氢钠 80 硫酸腺嘌呤 30 2ip 0.3 IAA,其中铁源由FeNa-EDDHA 代替硫酸亚铁和Na2EDTA?2H2O(mg/L) |
M462 |
MUSA (BANANA) MULTIPLICATION MEDIUM |
VUYLSTEKE培养基,包括植物生长调节剂,蔗糖和凝胶剂 |
M535 |
Murashige & Skoog (MS)Modified Basal Medium |
MS基本培养基,改良过的含硫胺素,肌醇,腺嘌呤硫酸盐,不含烟酸,盐酸吡哆醇和甘氨酸 |
B5培养基的主要特点是含有较低的铵,这是因为铵可能对不少培养物的生长有抑制作用。经过试验发现,有些植物的愈伤组织和悬浮培养物在MS培养基上生长得比B5培养基上要好,而另一些植物,在B5培养基上更适宜。 |
货号 |
名称 |
G398 |
Gamborg B5 Basal Medium |
B5基本培养基 含维生素 |
G768 |
Gamborg Basal Salt Mixture |
B5基本培养基 不含维生素 |
G249 |
Gamborg Vitamin Powder (1000x) |
B5维生素粉末 1000x |
G219 |
Gamborg Vitamin Solution (1000x) |
B5维生素溶液 1000x |
N492 |
NB Basal Medium |
N6大量元素与B5微量元素混合物 |
N6培养基特别则适合于禾谷类植物的花药和花粉培养,在国内外得到广泛应用。在组织培养中,经常采用的还有怀特(While,1963)培养基、尼许(Nitsch,1951)培养基等。它们在基本成分上大同小异。怀特培养基由于无机盐的数量比较低,更适合木本植物的组织培养。 |
货号 |
名称 |
C167 |
CHU’S N6 Basal Medium w/ Vitamins |
N6基本培养基 含维生素 |
C416 |
CHU’S N6 Basal Salt Mixture |
N6基本培养基 不含维生素 |
C149 |
CHU N6 Vitamin SOL. (1000x) |
N6基本培养基中维生素溶液 1000x |
其它培养基 |
S808 |
SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM(1/2x and 10 g/L Sucrose) |
Schenk & Hildebrandt 改良培养基含SH 1/2大量、微量和维生素,10g/L蔗糖 |
S806 |
SCHENK & HILDEBRANDT MODIFIED BASAL SALT MIXTURE |
Schenk & Hildebrandt 改良基盐混合物 不含氯化钙 |
L444 |
LLOYD & McCOWN WOODY PLANT MICRONUTRIENT MIXTURE |
LLOYD & McCOWN木本植物微量元素混合物/WPM微量元素 |
A267 |
ANDERSON BASAL SALT MIXTURE |
ANDERSON培养基基盐混合物 |
B144 |
BANANA AGS BASAL MEDIUM |
香蕉培养基含植物生长调节剂 |
B514 |
BLAYDES BASAL MEDIUM |
Blaydes 改良培养基基盐含蔗糖 |
B1396 |
Broadleaf Tree Basal Medium |
阔叶树(橡树、菩提树等)培养基基盐/Chalupa 培养基 |
C206 |
CAPE SUNDEW/ VENUS FLYTRAP MULTIPLICATION BASAL MEDIUM |
食肉植物培养基(如捕蝇草等)含植物生长调节剂 |
C212 |
CARROT CALLUS INTIATION MEDIUM FROM PITH TISSUE |
胡萝卜愈伤组织培养基 |
C222 |
CARROT SHOOT DEVELOPMENT BASAL MEDIUM |
胡萝卜幼苗发育培养基 |
C287 |
CHEE & POOL C2d VITIS BASAL MEDIUM |
Chee & Pool 培养基(葡萄幼苗) |
D146 |
DCR BASAL SALT MIXTURE |
DCR基盐混合物/Gupta and Durzan 培养基 |
D191 |
DKW BASAL MEDIUM, CONTAINS 10 g/L SUCROSE |
DKW培养基基盐,含10 g/L蔗糖 |
D189 |
DKW BASAL MEDIUM, CONTAINS 30 G/L SUCROSE |
DKW培养基基盐,含30 g/L蔗糖 |
D190 |
DKW BASAL SALT MIXTURE |
DKW培养基基盐 |
E575 |
ECONOMOU & READ BASAL MEDIUM |
ECONOMOU & READ 培养基 |
E333 |
EXS IIIa™ BASAL SALT MEDIUM WITH ADENINE |
EXS IIIa™ 培养基含腺嘌呤 |
E337 |
EXS III™ BASAL MEDIUM W/OUT ADENINE |
EXS III™ 不含腺嘌呤 |
G371 |
GRESSHOFF & DOY BASAL MEDIUM |
GRESSHOFF & DOY 培养基 |
H393 |
HELLER BASAL SALT MIXTURE |
HELLER 培养基基盐混合物 |
H396 |
HELLER/WHITE MODIFIED BASAL SALT MIXTURE |
HELLER/WHITE 改良培养基 不含三氧化钼 |
H353 |
HOAGLAND MODIFIED BASAL SALT MIXTURE |
Hoagland 改良培养基基盐混合物 |
H435 |
HOSTA INITIATION/MULTIPLICATION MEDIUM STAGE I/II MEDIUM |
HOSTA增殖培养基 I/II段 |
H436 |
HOSTA MULTIPLICATION MEDIUM STAGE II MEDIUM |
HOSTA增殖培养基 II段 改良的MS |
H437 |
HOSTA ROOTING MEDIUM STAGE III MEDIUM |
HOSTA生根培养基 II段 改良的MS |
K413 |
KAO & MICHAYLUK BASAL SALT MIXTURE |
Kao & Michayluk 培养基基盐 |
K427 |
KAO & MICHAYLUK MODIFIED BASAL MEDIUM |
Kao & Michayluk 改良培养基 |
L689 |
LINSMAIER & SKOOG BASAL MEDIUM |
LS 培养基(MS基盐和LS维生素)/MSMO 培养基 |
L467 |
LINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7 g/L AGAR |
LS 培养基 含30mg/L蔗糖,7g/L琼脂 |
L452 |
LINSMAIER & SKOOG BASAL MEDIUM W/ 30g/L SUCROSE & 7g/L AGAR, pH ADJUSTED & BUFFERED |
LS 培养基 含30mg/L蔗糖,7g/L琼脂和MES缓冲液 |
L473 |
LINSMAIER & SKOOG BASAL MODIFIED MEDIUM, w/ SUCROSE pH ADJUSTED AND BUFFERED |
LS 改良培养基 含30mg/L蔗糖和MES缓冲液 |
L477 |
LINSMAIER & SKOOG MODIFIED BASAL MEDIUM, pH ADJUSTED AND BUFFERED |
LS 改良培养基 含MES缓冲液 |
L546 |
LITVAY BASAL SALT MIXTURE |
Litvay 培养基基盐 |
L154 |
LLOYD & McCOWN WOODY PLANT BASAL SALT MIXTURE |
WPM (木本植物)培养基基盐混合物 |
L449 |
LLOYD & McCOWN WOODY PLANT MEDIUM w/ VITAMINS |
WPM (木本植物)培养基 含维生素 |
N616 |
NITSCH & NITSCH BASAL MEDIUM w/VITAMINS |
NN 培养基 |
N613 |
NITSCH & NITSCH BASAL SALT MIXTURE |
NN 培养基基盐混合物 |
N608 |
NITSCH & NITSCH VITAMIN POWDER (1000X) |
NN 培养基维生素(1000X) |
N479 |
NLN BASAL MEDIUM |
NLN培养基,不含蔗糖 |
P713 |
PARKER THOMPSON FERN BASAL SALT MIXTURE |
Parker Thompson 蕨类培养基基盐混合物 |
P6647 |
PHYTOTECH SPATHIPHYLLUM MEDIUM |
白鹤芋属植物培养基含凝胶剂,碳水化合物和植物生长调节剂 |
Q673 |
QUOIRIN & LEPOIVRE BASAL SALT MIXTURE |
Quoirin & Lepoivre 培养基基盐混合物 |
R756 |
ROSE INITIATION MEDIUM |
玫瑰培育培养基 |
R757 |
ROSE MODIFIED MULTIPLICATION BASAL MEDIUM |
玫瑰改良增殖培养基 |
R758 |
ROSE MODIFIED ROOTING BASAL MEDIUM |
玫瑰改良生根培养基 |
R7100 |
RUGINI OLIVE MEDIUM |
Rugini 橄榄树培养基 |
S813 |
SCHENK & HILDEBRANDT BASAL MEDIUM |
Schenk and Hildebrandt 培养基 |
S816 |
Schenk & Hildebrandt Basal Salt Mixture |
Schenk & Hildebrandt 培养基基盐混合物 |
S811 |
Schenk & Hildebrandt Modified Basal Medium |
Schenk & Hildebrandt 改良培养基基盐混合物含10g/L蔗糖 |
T7954 |
TM4 Basal Medium |
TM4培养基(西红柿原生质体培养基) |
T8024 |
TM4 Basal Salts |
TM4培养基基盐(西红柿原生质体培养基) |
T868 |
TM4G Basal Medium |
TM4G 培养基 |
T853 |
TM4G Basal Salt Mixture |
TM4G 培养基基盐 |
T856 |
Tobacco Modified Callus Initiation Basal Medium |
改良的烟草愈伤组织培养基 |
T861 |
Tobacco Modified Root Initiation Basal Medium |
改良的烟草生根培养基 |
T867 |
Tobacco Modified Shoot and Root Basal Medium |
改良的烟草幼苗和生根培养基 |
T864 |
Tobacco Modified Shoot Multiplication Basal Medium |
改良的烟草幼苗微量增殖培养基 |
W863 |
Westvaco WV3 Basal Medium |
Coke培养基/Westvaco WV3培养基 |
W865 |
Westvaco WV5 Basal Medium |
Coke培养基/Westvaco WV5培养基 |
W898 |
White Basal Salt Mixture |
White 培养基 |
维生素 |
E330 |
Eriksson Vitamin Solution (1000x) |
Eriksson 维生素溶液(1000x) |
K421 |
Kao & Michayluk Vitamin Solution (100x) |
Kao and Michayluk 维生素溶液(100x) 已滤灭 |
M587 |
MOREL & MARTIN VITAMIN SOLUTION |
Morel & Martin 维生素溶液(100x) |
M592 |
Morel & Wetmore Vitamin Solution (100X) |
Morel & Wetmore 维生素溶液(100x) |
N608 |
Nitsch & Nitsch Vitamin Powder (1000x) |
Nitsch & Nitsch 维生素粉末(1000x) |
N603 |
Nitsch & Nitsch Vitamin Solution (1000x) |
Nitsch & Nitsch 维生素溶液(1000x) |
S826 |
Schenk & Hildebrandt Vitamin Powder (100x) |
Schenk & Hildebrandt 维生素粉末(100x) |
S743 |
Staba Modified Vitamin Solution (100x) |
改良的 Staba 维生素溶液(100x) |