Recombinant Murine KGF-2/FGF-10
产品编号: P6092-1mg
产品包装:1mg
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5μg 100μg 1mg
数
量
Recombinant Murine KGF-2/FGF-10(重组小鼠KGF-2/FGF-10)(P6092-1mg)
说明书下载
价格: ¥ 23581.00
产品简介
使用说明
产品文件
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产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P6092-5μg | Recombinant Murine KGF-2/FGF-10 | 5μg | 669.00元 |
| P6092-100μg | Recombinant Murine KGF-2/FGF-10 | 100μg | 7363.00元 |
| P6092-1mg | Recombinant Murine KGF-2/FGF-10 | 1mg | 23581.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Mouse | 14165 | O35565 | E. coli | 173aa | 19.5kDa | － |
| About this Antibody | |
| Name | Recombinant Murine KGF-2/FGF-10 (Recombinant Murine Keratinocyte Growth Factor-2/FGF-10; rMuKGF-2/FGF-10)；Recombinant Mouse KGF-2/FGF-10 ; 重组小鼠角质细胞生长因子2/成纤维细胞生长因子10 |
| Synonyms | KGF-2; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria |
| Purity | >95% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5ng/ml, corresponding to a specific activity of >2.0×106IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, 600mM NaCl, pH7.4, 1mM mercaptoethanol. |
| Endotoxin | Less than 1EU/μg of rMuKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family, which is involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may take parts in wound healing and is required for normal branching morphogenesis. Recombinant murine FGF-10 contains a 173 amino acids and it shares 93% and 90%a.a. sequence identity with human and rat FGF-10. |
| Amino Acid Sequence | QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P6092-5μg | Recombinant Murine KGF-2/FGF-10 | 5μg |
| P6092-25μg | Recombinant Murine KGF-2/FGF-10 | 25μg |
| P6092-100μg | Recombinant Murine KGF-2/FGF-10 | 100μg |
| P6092-1mg | Recombinant Murine KGF-2/FGF-10 | 1mg |
| – | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
相关产品请点击如下按钮：
细胞因子
培养液相关
使用本产品的文献：
Mouse KGF-1/FGF-7 小鼠KGF-1/FGF-7
Mouse KGF-1/FGF-7 小鼠KGF-1/FGF-7
产品说明书
FAQ
COA
已发表文献
产品描述
成纤维生长因子7（FGF-7），也称为角质形成细胞生长因子 (KGF) ，是成纤维细胞生长因子 (FGF) 家族的成员。在胚胎发育、细胞增殖和细胞分化的调控中发挥重要作用。正常分枝形态发生所必需的，对角质形成细胞有活性的生长因子。可能是正常上皮细胞增殖的主要旁分泌效应物。在大肠杆菌中过表达的重组KGF活性约为天然蛋白的10倍，可能与重组KGF未被糖基化有关。KGF，122-132AA段是KGF受体的特异性结合位点。KGF由各种来源的间质细胞分泌，与肝素有较强的亲和力，其受体KGFR属于蛋白酪氨酸激酶受体家族，其主要分布于上皮细胞，KGF与靶细胞膜上的受体KGFR特异性结合后，促使受体自身磷酸化，从而启动细胞内信号级联反应，进而发挥多种生物学功能:参与组织器官发育、促进细胞增殖及组织损伤修复等。
本品是由大肠杆菌原核表达系统制备的重组蛋白，经分离纯化、过滤除菌和冻干等步骤制成，具有高纯度、高活性、无标签及低内毒素等特点。
性能参数
|
分子别名 |
Fibroblast growth factor 7；HBGF-7 |
|
表达系统及表达区间 |
Mouse FGF-7 is expressed from E.coli with no tag . It contains Cys32-Thr194 |
|
AA Sequence |
CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT |
|
Accession |
P36363 |
|
分子量 |
Approximately 18.7 kDa. |
|
生物活性 |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
|
外观 |
无菌过滤白色冻干粉末。 |
|
纯度 |
> 96%，SDA-PAGE和 HPLC 检测 |
|
内毒素 |
<1.0 EU/μg |
|
制剂 |
Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl. |
|
复溶方法 |
建议在打开之前短暂离心，以将内容物带到底部。在无菌蒸馏水或者含有0.1% BSA的水性缓冲液中重新配制至0.1-1.0 mg/mL的浓度。储备溶液应分成等分试样，并在≤-20°C下储存。 |
储存条件
-25~-15℃保存，收到货之后有效期1年。
复溶后， 2 ~8 °C保存，30天有效期。复溶后， -25~-15℃保存，3个月有效期。
建议首次使用时将蛋白等分，避免重复冻融循环。
注意事项
1. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
Ver.CN20240305
产品描述
成纤维生长因子7（FGF-7），也称为角质形成细胞生长因子 (KGF) ，是成纤维细胞生长因子 (FGF) 家族的成员。在胚胎发育、细胞增殖和细胞分化的调控中发挥重要作用。正常分枝形态发生所必需的，对角质形成细胞有活性的生长因子。可能是正常上皮细胞增殖的主要旁分泌效应物。在大肠杆菌中过表达的重组KGF活性约为天然蛋白的10倍，可能与重组KGF未被糖基化有关。KGF，122-132AA段是KGF受体的特异性结合位点。KGF由各种来源的间质细胞分泌，与肝素有较强的亲和力，其受体KGFR属于蛋白酪氨酸激酶受体家族，其主要分布于上皮细胞，KGF与靶细胞膜上的受体KGFR特异性结合后，促使受体自身磷酸化，从而启动细胞内信号级联反应，进而发挥多种生物学功能:参与组织器官发育、促进细胞增殖及组织损伤修复等。
本品是由大肠杆菌原核表达系统制备的重组蛋白，经分离纯化、过滤除菌和冻干等步骤制成，具有高纯度、高活性、无标签及低内毒素等特点。
性能参数
|
分子别名 |
Fibroblast growth factor 7；HBGF-7 |
|
表达系统及表达区间 |
Mouse FGF-7 is expressed from E.coli with no tag . It contains Cys32-Thr194 |
|
AA Sequence |
CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT |
|
Accession |
P36363 |
|
分子量 |
Approximately 18.7 kDa. |
|
生物活性 |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
|
外观 |
无菌过滤白色冻干粉末。 |
|
纯度 |
> 96%，SDA-PAGE和 HPLC 检测 |
|
内毒素 |
<1.0 EU/μg |
|
制剂 |
Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl. |
|
复溶方法 |
建议在打开之前短暂离心，以将内容物带到底部。在无菌蒸馏水或者含有0.1% BSA的水性缓冲液中重新配制至0.1-1.0 mg/mL的浓度。储备溶液应分成等分试样，并在≤-20°C下储存。 |
储存条件
-25~-15℃保存，收到货之后有效期1年。
复溶后， 2 ~8 °C保存，30天有效期。复溶后， -25~-15℃保存，3个月有效期。
建议首次使用时将蛋白等分，避免重复冻融循环。
注意事项
1. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
Ver.CN20240305
暂无内容
暂无内容
Mouse KGF-2/FGF-10 重组小鼠角质形成细胞生长因子-2/FGF-10(小鼠KGF-2/FGF-10)
Mouse KGF-2/FGF-10 重组小鼠角质形成细胞生长因子-2/FGF-10(小鼠KGF-2/FGF-10)
产品说明书
FAQ
COA
已发表文献
Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is a stromal-derived paracrine growth factor considered to be important during embryogenesis. FGF-10 plays an important role in adipogenesis, at least partly by contributing to the expression of C/EBPβ through an autocrine/paracrine mechanism. During cardiogenesis, FGF-10 is expressed in the anterior second heart field. Together with Fibroblast growth factor 8 (FGF-8), FGF10 promotes the proliferation of these cardiac progenitor cells that form the arterial pole of the heart.
Product Properties
|
Synonyms |
FGF10, FGF-10, fibroblast growth factor 10, Keratinocyte growth factor 2, KGF2, KGF-2 |
|
Accession |
O35565 |
|
GeneID |
14165 |
|
Source |
E.coli-derived mouse KGF-2/FGF-10, Gln37-Thr209. |
|
Molecular Weight |
Approximately 19.5 kDa. |
|
AA Sequence |
QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
>95% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
HB220214
Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is a stromal-derived paracrine growth factor considered to be important during embryogenesis. FGF-10 plays an important role in adipogenesis, at least partly by contributing to the expression of C/EBPβ through an autocrine/paracrine mechanism. During cardiogenesis, FGF-10 is expressed in the anterior second heart field. Together with Fibroblast growth factor 8 (FGF-8), FGF10 promotes the proliferation of these cardiac progenitor cells that form the arterial pole of the heart.
Product Properties
|
Synonyms |
FGF10, FGF-10, fibroblast growth factor 10, Keratinocyte growth factor 2, KGF2, KGF-2 |
|
Accession |
O35565 |
|
GeneID |
14165 |
|
Source |
E.coli-derived mouse KGF-2/FGF-10, Gln37-Thr209. |
|
Molecular Weight |
Approximately 19.5 kDa. |
|
AA Sequence |
QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
>95% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
HB220214
暂无内容
暂无内容