pcDNA3.1(hyg)+载体说明书

 

pcDNA3.1(hyg)+

型号 载体名称 出品公司 载体用途
VPI0008 pcDNA3.1(hyg)+ Invitrogen 哺乳动物表达载体

 

产品参数: 

Promotor: CMV
5′ Sequencing 1 Primer: T7 Fwd
5′ Sequencing 1 Primer Sequence: 5’d[TAATACGACTCACTATAGGG]3′
Selectable Marker: Hygromycin

载体描述: 

Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.

说明书: 
 pcDNA3.1(hyg)+ 载体序列
质粒图谱: 

Clontech pTK-hyg (VSC0467)说明书

 

pTK-hyg

型号 载体名称 出品公司 载体用途
VSC0467 pTK-hyg Clontech 四环素调控系统

 

Description

pTK-Hyg is a selection vector that confers hygromycin resistance in mammalian cells for the 

selection of stably transformed cells. pTK-Hyg is especially useful for selection of double-stable 

cell lines using the Tet-On® or Tet-Off® Gene Expression Systems because the absence of an 

enhancer element on pTK-Hyg prevents the unwanted activation of pTRE- and pBI-derived 

plasmids upon cointegration into the host cell’s genome.

Use

pTK-Hyg can be cotransfected into the host cell line with an expression plasmid that contains 

a gene of interest using any standard transfection technique. Most mammalian cell lines 

require 200 µg/ml of Hygromycin to select for stably transformed cells.

Location of Features

HSV TK polyA signals: 1473–1478 & 1486–1491 (complementary)

Hygr

 (hygromycin resistance gene): 1537–2574 (complementary)

P

HSV TK (HSV TK promoter): 2588–2835 (complementary)

pUC origin ofreplication: 3152–3795

Ampr

 (ampicillin resistance gene; beta-lactamase): 3943–4803

Propagation in E. coli

Suitable host strains: DH™ and other general purpose strains. 

• Selectable marker: plasmid confers resistance to ampicillin (50 µg/ml) on E. coli hosts.

• E. coli replication origin: pUC

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