Molecular Innovations GAM-HRP 说明书

世界*实验材料供应商 Molecular Innovations上海金畔生物为其中国代理, Molecular Innovations在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Molecular Innovations就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Molecular Innovations中国代理, Molecular Innovations上海代理, Molecular Innovations北京代理,Molecular Innovations广东代理, Molecular Innovations江苏代理Molecular Innovations湖北代理,Molecular Innovations天津,Molecular Innovations黑龙江代理,Molecular Innovations内蒙古代理,Molecular Innovations吉林代理,Molecular Innovations福建代理, Molecular Innovations江苏代理, Molecular Innovations浙江代理, Molecular Innovations四川代理,

 

美国Molecular Innovations公司成立于1990年,是一家致力于抗体、纯化蛋白质及ELISA 试剂盒等产品的研发与生产的大型生物技术企业。该公司在凝血酶及溶栓剂研究领域有着*的优势,其提供的产品有纯化蛋白、单/多克隆抗体、ELISA检测试剂盒、抑制剂与荧光底物、层析树脂、血浆制品及组织切片六大产品系列,主要包括纤溶酶原激活物抑制因子1,肾素,肾素原,玻连蛋白,纤维蛋白,纤维连接蛋白,血纤维蛋白溶酶原,血纤维蛋白溶酶,抗凝血酶,多款ELISA试剂盒,抗体和其他研究使用的试剂。高质量的研究产品保证了研究人员检测操作方便快捷,结果、稳定。

 

Anti Mouse IgG, HRP Labeled

抗鼠IgG,HRP标记

规格:0.05ml.0.01ml

货号:GAM-HRP 分类:二抗,ELISA组件

 

产品描述

抗鼠IgG,HRP标记

辣根过氧化物酶缀合的山羊抗小鼠IgG,重链和轻链特异性。亲和纯化并吸附牛,马和人血清蛋白。通过对正常小鼠血清的免疫电泳测定,小鼠IgG具有单特异性,重链和轻链。通过直接固相免疫测定,与正常牛,马和人血清的交叉反应<2%。推荐用于ELISA的初始稀释度为1:10,000,但是zui终用户必须优化适当的工作稀释度。 

 

附加信息

纯度

亲和纯化

资源

山羊血清

目标分子量

160000

抗原

全长天然蛋白质(纯化的)(小鼠)。

表位

识别鼠IgG,重链和轻链

交叉反应性

女士

克隆

多克隆

滴度

1:10,000

主办

山羊

存储

4 C

运输条件

冷包

呼气

交货12个月

形成

液体

缓冲

0.01M PBS; 0.0005M EDTA; 10%甘露糖; 2%蔗糖; 1%BSA; 0.02%硫柳汞; pH 7.6

防腐剂

硫柳汞

样本大小

0.01毫升

样品浓度

1.0毫克/毫升

应用

ELISA

 

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

产品说明书

FAQ

COA

已发表文献

产品简介

本品是由辣根过氧化酶(HRP)标记的山羊抗兔IgG(H+L),使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子,也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应,但不会识别非免疫球蛋白类的血清蛋白。

本品适合做单标实验。要做多标(Multiple-labeling)实验,建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

 

产品应用

建议稀释浓度:

IHC/ICC:1:500-1:5000;

ELISA/WB(显色法):1:5000-1:100000;

ELISA/WB(化学发光法):1:10000-1:200000;

 

产品信息

抗体浓度(Antibody Concentration)

100 μL(0.4 mg/mL)

缓冲液(Buffer)

0.005M 磷酸钠,0.125M 氯化钠,pH7.6

稳定剂(Stabilizer)

7.5 mg/mL BSA(无IgG,蛋白酶), 50%甘油

防腐剂(Preservative)

None(叠氮化钠会抑制HRP活性)

原料来源(Source of Material)

Jackson Immunoresearch 111-035-003

 

储存条件

-25 ~ -15℃分装保存,尽量避免反复冻融。有效期1年。

 

注意事项

1)本品不能保存于叠氮化钠中其会抑制辣根过氧化酶(HRP)活性;

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

3)本产品仅作科研用途!

 

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

暂无内容

[1] Wang Z, Lu Z, Lin S, et al. Leucine-tRNA-synthase-2-expressing B cells contribute to colorectal cancer immunoevasion. Immunity. 2022;55(6):1067-1081.e8. doi:10.1016/j.immuni.2022.04.017(IF:43.474)
[2] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)
[3] Yang WQ, Xiong QP, Ge JY, et al. THUMPD3-TRMT112 is a m2G methyltransferase working on a broad range of tRNA substrates. Nucleic Acids Res. 2021;49(20):11900-11919. doi:10.1093/nar/gkab927(IF:16.971)
[4] Qian Y, Liu Q, Li P, et al. Highly Tumor-Specific and Long-Acting Iodine-131 Microbeads for Enhanced Treatment of Hepatocellular Carcinoma with Low-Dose Radio-Chemoembolization. ACS Nano. 2021;15(2):2933-2946. doi:10.1021/acsnano.0c09122(IF:15.881)
[5] Wang K, Jiang L, Hu A, et al. Vertebral-specific activation of the CX3CL1/ICAM-1 signaling network mediates non-small-cell lung cancer spinal metastasis by engaging tumor cell-vertebral bone marrow endothelial cell interactions. Theranostics. 2021;11(10):4770-4789. Published 2021 Mar 4. doi:10.7150/thno.54235(IF:11.556)
[6] Han L, Lai H, Yang Y, et al. A 5'-tRNA halve, tiRNA-Gly promotes cell proliferation and migration via binding to RBM17 and inducing alternative splicing in papillary thyroid cancer. J Exp Clin Cancer Res. 2021;40(1):222. Published 2021 Jul 5. doi:10.1186/s13046-021-02024-3(IF:11.161)
[7] Gao JP, Xu P, Wang M, et al. Nod factor receptor complex phosphorylates GmGEF2 to stimulate ROP signaling during nodulation. Curr Biol. 2021;31(16):3538-3550.e5. doi:10.1016/j.cub.2021.06.011(IF:10.834)
[8] Wang T, Hu J, Luo H, et al. Photosensitizer and Autophagy Promoter Coloaded ROS-Responsive Dendrimer-Assembled Carrier for Synergistic Enhancement of Tumor Growth Suppression. Small. 2018;14(38):e1802337. doi:10.1002/smll.201802337(IF:9.598)
[9] Wu X, Qiao S, Wang W, et al. Melatonin prevents peri‑implantitis via suppression of TLR4/NF-κB. Acta Biomater. 2021;134:325-336. doi:10.1016/j.actbio.2021.07.017(IF:8.947)
[10] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment. J Immunother Cancer. 2019;7(1):327. Published 2019 Nov 28. doi:10.1186/s40425-019-0750-6(IF:8.728)
[11] Song Y, Guo Y, Li X, et al. RBM39 Alters Phosphorylation of c-Jun and Binds to Viral RNA to Promote PRRSV Proliferation. Front Immunol. 2021;12:664417. Published 2021 May 17. doi:10.3389/fimmu.2021.664417(IF:7.561)
[12] Wang C, Qu L, Li S, et al. Discovery of First-in-Class Dual PARP and EZH2 Inhibitors for Triple-Negative Breast Cancer with Wild-Type BRCA. J Med Chem. 2021;64(17):12630-12650. doi:10.1021/acs.jmedchem.1c00567(IF:7.446)
[13] Song M, Wang Y, Chen Z, et al. Human CYP enzyme-activated genotoxicity of 2,2',4,4'-tetrabromobiphenyl ether in mammalian cells. Chemosphere. 2022;291(Pt 1):132784. doi:10.1016/j.chemosphere.2021.132784(IF:7.086)
[14] Liu S, Xu DS, Li M, et al. Icariin attenuates endothelial-mesenchymal transition via H19/miR-148b-3p/ELF5 in ox-LDL-stimulated HUVECs. Mol Ther Nucleic Acids. 2020;23:464-475. Published 2020 Dec 3. doi:10.1016/j.omtn.2020.11.021(IF:7.032)
[15] Wang C, Zhang H, Fu J, et al. Bartonella type IV secretion effector BepC induces stress fiber formation through activation of GEF-H1. PLoS Pathog. 2021;17(1):e1009065. Published 2021 Jan 28. doi:10.1371/journal.ppat.1009065(IF:6.823)
[16] Liu W, Guan Y, Qiao S, et al. Antiaging Effects of Vicatia thibetica de Boiss Root Extract on Caenorhabditis elegans and Doxorubicin-Induced Premature Aging in Adult Mice. Oxid Med Cell Longev. 2021;2021:9942090. Published 2021 Aug 6. doi:10.1155/2021/9942090(IF:6.543)
[17] Qu L, Li S, Ji L, et al. Discovery of PT-65 as a highly potent and selective Proteolysis-targeting chimera degrader of GSK3 for treating Alzheimer's disease. Eur J Med Chem. 2021;226:113889. doi:10.1016/j.ejmech.2021.113889(IF:6.514)
[18] Lao Y, Wang Y, Chen J, et al. Synthesis and biological evaluation of 1,2,4-triazole derivatives as potential Nrf2 activators for the treatment of cerebral ischemic injury. Eur J Med Chem. 2022;236:114315. doi:10.1016/j.ejmech.2022.114315(IF:6.514)
[19] Wang C, Chen X, Liu X, et al. Discovery of precision targeting EZH2 degraders for triple-negative breast cancer. Eur J Med Chem. 2022;238:114462. doi:10.1016/j.ejmech.2022.114462(IF:6.514)
[20] Qu L, Ji L, Wang C, et al. Synthesis and evaluation of multi-target-directed ligands with BACE-1 inhibitory and Nrf2 agonist activities as potential agents against Alzheimer's disease. Eur J Med Chem. 2021;219:113441. doi:10.1016/j.ejmech.2021.113441(IF:6.514)
[21] Bu S, Wang Q, Sun J, Li X, Gu T, Lai D. Melatonin suppresses chronic restraint stress-mediated metastasis of epithelial ovarian cancer via NE/AKT/β-catenin/SLUG axis [published correction appears in Cell Death Dis. 2020 Sep 8;11(9):726]. Cell Death Dis. 2020;11(8):644. Published 2020 Aug 18. doi:10.1038/s41419-020-02906-y(IF:6.304)
[22] Ning FL, Tao J, Li DD, et al. Activating BK channels ameliorates vascular smooth muscle calcification through Akt signaling. Acta Pharmacol Sin. 2022;43(3):624-633. doi:10.1038/s41401-021-00704-6(IF:6.150)
[23] Wang C, Zhang J, Song S, et al. Imaging epileptic foci in mouse models via a low-density lipoprotein receptor-related protein-1 targeting strategy. EBioMedicine. 2021;63:103156. doi:10.1016/j.ebiom.2020.103156(IF:5.736)
[24] Xu R, Lai C, Yang F, et al. Preliminary Characterization of Two Small Insulinase-Like Proteases in Cryptosporidium parvum. Front Microbiol. 2021;12:651512. Published 2021 May 21. doi:10.3389/fmicb.2021.651512(IF:5.640)
[25] Su J, Jin C, Wu H, et al. Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins. Front Microbiol. 2019;10:1177. Published 2019 May 29. doi:10.3389/fmicb.2019.01177(IF:5.640)
[26] Zhang S, Wang Y, Wu H, et al. Characterization of a Species-Specific Insulinase-Like Protease in Cryptosporidium parvum. Front Microbiol. 2019;10:354. Published 2019 Mar 6. doi:10.3389/fmicb.2019.00354(IF:5.640)
[27] Yang R, Li D, Yi S, Wang M. Evolutionarily conserved odorant-binding proteins participate in establishing tritrophic interactions. iScience. 2022;25(7):104664. Published 2022 Jun 23. doi:10.1016/j.isci.2022.104664(IF:5.458)
[28] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 exerts potent antitumor activity and synergizes with standard-of-care agents in preclinical acute myeloid leukemia models. Cell Death Discov. 2021;7(1):90. Published 2021 May 3. doi:10.1038/s41420-021-00465-5(IF:5.241)
[29] Ma Y, Zheng K, Pang Y, et al. Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs. J Orthop Translat. 2021;32:59-68. Published 2021 Jun 2. doi:10.1016/j.jot.2021.05.002(IF:5.191)
[30] Wu X, Jin S, Yang Y, et al. Altered expression of ferroptosis markers and iron metabolism reveals a potential role of ferroptosis in vitiligo. Pigment Cell Melanoma Res. 2022;35(3):328-341. doi:10.1111/pcmr.13032(IF:4.693)
[31] Li Z, Liu S, Fu T, Peng Y, Zhang J. Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):351. Published 2019 Nov 27. doi:10.1186/s13287-019-1441-4(IF:4.627)
[32] Shi X, Ding K, Zhao Q, et al. Suppression of CPSF6 Enhances Apoptosis Through Alternative Polyadenylation-Mediated Shortening of the VHL 3'UTR in Gastric Cancer Cells. Front Genet. 2021;12:707644. Published 2021 Sep 14. doi:10.3389/fgene.2021.707644(IF:4.599)
[33] Chen Z, Li Z, Jiang C, Jiang X, Zhang J. MiR-92b-3p promotes neurite growth and functional recovery via the PTEN/AKT pathway in acute spinal cord injury. J Cell Physiol. 2019;234(12):23043-23052. doi:10.1002/jcp.28864(IF:4.522)
[34] Yao H, Xu K, Zhou J, Zhou L, Wei S. A Tumor Microenvironment Destroyer for Efficient Cancer Suppression. ACS Biomater Sci Eng. 2020;6(1):450-462. doi:10.1021/acsbiomaterials.9b01544(IF:4.511)
[35] Sun X, Zhang J, Nie Q. Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples. PLoS Comput Biol. 2021;17(3):e1008379. Published 2021 Mar 5. doi:10.1371/journal.pcbi.1008379(IF:4.475)
[36] Yin L, Zhang L, Luo L, et al. Berbamine reduces body weight via suppression of small GTPase Rab8a activity and activation of paraventricular hypothalamic neurons in obese mice. Eur J Pharmacol. 2022;916:174679. doi:10.1016/j.ejphar.2021.174679(IF:4.432)
[37] Fang DD, Zhu H, Tang Q, et al. FLT3 inhibition by olverembatinib (HQP1351) downregulates MCL-1 and synergizes with BCL-2 inhibitor lisaftoclax (APG-2575) in preclinical models of FLT3-ITD mutant acute myeloid leukemia. Transl Oncol. 2022;15(1):101244. doi:10.1016/j.tranon.2021.101244(IF:4.243)
[38] Li D, Qiu X, Yang J, Liu T, Luo Y, Lu Y. Generation of Human Lens Epithelial-Like Cells From Patient-Specific Induced Pluripotent Stem Cells. J Cell Physiol. 2016;231(12):2555-2562. doi:10.1002/jcp.25374(IF:4.155)
[39] Su J, Shen Y, Li N, et al. Comparative Characterization of CpCDPK1 and CpCDPK9, Two Potential Drug Targets Against Cryptosporidiosis. Microorganisms. 2022;10(2):333. Published 2022 Feb 1. doi:10.3390/microorganisms10020333(IF:4.128)
[40] Guo Y, Huo J, Wu D, et al. Simvastatin inhibits the adipogenesis of bone marrow‑derived mesenchymal stem cells through the downregulation of chemerin/CMKLR1 signaling. Int J Mol Med. 2020;46(2):751-761. doi:10.3892/ijmm.2020.4606(IF:4.101)
[41] Yang B, Li Y, Ma Y, et al. Selenium attenuates ischemia/reperfusion injury‑induced damage to the blood‑brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway‑mediated autophagy inhibition. Int J Mol Med. 2021;48(3):178. doi:10.3892/ijmm.2021.5011(IF:4.101)
[42] Liu S, Xu DS, Ma JL, Huang P, Wu D, Ren LQ. LncRNA H19 Mitigates Oxidized Low-Density Lipoprotein Induced Pyroptosis via Caspase-1 in Raw 264.7 Cells. Inflammation. 2021;44(6):2407-2418. doi:10.1007/s10753-021-01511-1(IF:4.092)
[43] Zhang H, Guo F, Qi P, et al. OsHDA710-Mediated Histone Deacetylation Regulates Callus Formation of Rice Mature Embryo. Plant Cell Physiol. 2020;61(9):1646-1660. doi:10.1093/pcp/pcaa086(IF:4.062)
[44] Sun T, Zhang L, Feng J, et al. Characterization of cellular senescence in doxorubicin-induced aging mice. Exp Gerontol. 2022;163:111800. doi:10.1016/j.exger.2022.111800(IF:4.032)
[45] Wang J, Zhang Y, Liu X, Liu H. Is the Fixed Periodic Treatment Effective for the Tumor System without Complete Information?. Cancer Manag Res. 2021;13:8915-8928. Published 2021 Nov 30. doi:10.2147/CMAR.S339787(IF:3.989)
[46] Chen M, Lin H, Gao Y, Wang Z, Li Y, Jin F. Ghrelin attenuates drowning injury via dual effects on damage protection and immune repression. Ann Transl Med. 2021;9(11):920. doi:10.21037/atm-21-795(IF:3.932)
[47] Shi L, Zhang XB, Shi YF, et al. OsCDC48/48E complex is required for plant survival in rice (Oryza sativa L.). Plant Mol Biol. 2019;100(1-2):163-179. doi:10.1007/s11103-019-00851-9(IF:3.928)
[48] Shi J, Wang Y, Chen J, et al. Synthesis and biological evaluation of 1,2,4-oxadiazole core derivatives as potential neuroprotectants against acute ischemic stroke. Neurochem Int. 2021;148:105103. doi:10.1016/j.neuint.2021.105103(IF:3.921)
[49] Zhang KW, Wang D, Cai H, et al. IL‑6 plays a crucial role in epithelial‑mesenchymal transition and pro‑metastasis induced by sorafenib in liver cancer. Oncol Rep. 2021;45(3):1105-1117. doi:10.3892/or.2021.7926(IF:3.906)
[50] Qu F, Song Y, Wu Y, et al. The protective role of Ephrin-B2/EphB4 signaling in osteogenic differentiation under inflammatory environment. Exp Cell Res. 2021;400(2):112505. doi:10.1016/j.yexcr.2021.112505(IF:3.905)
[51] Cheng Q, Fan X, Liu Y, et al. miR-455-5p regulates circadian rhythms by accelerating the degradation of Clock mRNA. IUBMB Life. 2022;74(3):245-258. doi:10.1002/iub.2587(IF:3.885)
[52] He RZ, Zheng JH, Yao HF, et al. ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer [published online ahead of print, 2022 Apr 25]. Hepatobiliary Pancreat Dis Int. 2022;S1499-3872(22)00117-5. doi:10.1016/j.hbpd.2022.04.005(IF:3.780)
[53] Wang Y, Wang H, Zhang L, et al. Potential mechanisms of tremor tolerance induced in rats by the repeated administration of total alkaloid extracts from the seeds of Peganum harmala Linn. J Ethnopharmacol. 2020;262:113183. doi:10.1016/j.jep.2020.113183(IF:3.690)
[54] Bu S, Li B, Wang Q, et al. Epithelial ovarian cancer stem‑like cells are resistant to the cellular lysis of cytokine‑induced killer cells via HIF1A‑mediated downregulation of ICAM‑1. Int J Oncol. 2019;55(1):179-190. doi:10.3892/ijo.2019.4794(IF:3.571)
[55] Shen Y, Zhou M, Yan J, et al. miR-200b inhibits TNF-α-induced IL-8 secretion and tight junction disruption of intestinal epithelial cells in vitro. Am J Physiol Gastrointest Liver Physiol. 2017;312(2):G123-G132. doi:10.1152/ajpgi.00316.2016(IF:3.468)
[56] Yao C, Chen X, Xu Y, et al. Comparing pretreatment strategies to increase the yield and purity of human urinary extracellular vesicles [published online ahead of print, 2022 Jun 30]. J Chromatogr B Analyt Technol Biomed Life Sci. 2022;1206:123359. doi:10.1016/j.jchromb.2022.123359(IF:3.318)
[57] Cao H, Hua D, Zhang H, et al. Oral immunization of recombinant Saccharomyces cerevisiae expressing fiber-2 of fowl adenovirus serotype 4 induces protective immunity against homologous infection. Vet Microbiol. 2022;271:109490. doi:10.1016/j.vetmic.2022.109490(IF:3.293)
[58] Li Q , Sun J , Mohammadtursun N , Wu J , Dong J , Li L . Curcumin inhibits cigarette smoke-induced inflammation via modulating the PPARγ-NF-κB signaling pathway. Food Funct. 2019;10(12):7983-7994. doi:10.1039/c9fo02159k(IF:3.241)
[59] Yang LY, Liu XF, Yang Y, et al. Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities. Acta Pharmacol Sin. 2017;38(1):56-68. doi:10.1038/aps.2016.89(IF:3.166)
[60] Fei J, Wu H, Su J, et al. Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum. Parasit Vectors. 2018;11(1):312. Published 2018 May 23. doi:10.1186/s13071-018-2889-2(IF:3.163)
[61] Yao Y, Luo R, Xiong S, Zhang C, Zhang Y. Protective effects of curcumin against rat intestinal inflammation‑related motility disorders. Mol Med Rep. 2021;23(5):391. doi:10.3892/mmr.2021.12030(IF:2.952)
[62] Li X, An P, Zhao Y, et al. Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells. Evid Based Complement Alternat Med. 2022;2022:3664696. Published 2022 Apr 22. doi:10.1155/2022/3664696(IF:2.630)
[63] Li D, Han X, Zhao Z, Lu Y, Yang J. Functional analysis of deleterious EPHA2 SNPs in lens epithelial cells. Mol Vis. 2021;27:384-395. Published 2021 Jun 23. (IF:2.367)
[64] You J, Cheng Y, Yang XJ, Chen L. Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system. Stem Cell Res. 2021;53:102342. doi:10.1016/j.scr.2021.102342(IF:2.020)
[65] You J, Xi H, Ma S, Yang XJ, Chen L. Generation of a homozygous LRPAP1 knockout human embryonic stem cell line (FDCHDPe009-B) by CRISPR/Cas9 system. Stem Cell Res. 2021;56:102516. doi:10.1016/j.scr.2021.102516(IF:2.020)
[66] Zhu T, Zhao Y, Wu Y, Qu D. The Staphylococcus epidermidis gdpS regulates biofilm formation independently of its protein-coding function. Microb Pathog. 2017;105:264-271. doi:10.1016/j.micpath.2017.02.045(IF:2.009)
[67] Liu X, Xie F, Lai G, Wang J. Roles of heterogeneous nuclear ribonucleoprotein L in enamel organ development and the differentiation of ameloblasts. Arch Oral Biol. 2020;120:104933. doi:10.1016/j.archoralbio.2020.104933(IF:1.931)
[68] Jiang J, Chen Z, Yang Y, Yan J, Jiang H. Sevoflurane downregulates IGF‑1 via microRNA‑98. Mol Med Rep. 2017;15(4):1863-1868. doi:10.3892/mmr.2017.6219(IF:1.692)
[69] Xia Q, Zhou Y, Wang X, Fu S. Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis. Exp Ther Med. 2018;15(2):1586-1593. doi:10.3892/etm.2017.5549(IF:1.261)
[70] Li X, Lei Y, Yu Y, et al. Discovery and characterization of a novel splice variant of the p53 tumor suppressor gene in a human T cell leukemia cellline. Int J Clin Exp Pathol. 2020;13(5):1121-1135. Published 2020 May 1. (IF:0.252)
[71] Feng Y, Jiang C, Yang F, Chen Z, Li Z. Apocynum venetum leaf extract protects against H2O2-induced oxidative stress by increasing autophagy in PC12 cells. Biomed Rep. 2020;13(2):6. doi:10.3892/br.2020.1313(IF:0.000)

产品简介

本品是由辣根过氧化酶(HRP)标记的山羊抗兔IgG(H+L),使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子,也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应,但不会识别非免疫球蛋白类的血清蛋白。

本品适合做单标实验。要做多标(Multiple-labeling)实验,建议使用经过与相近物种血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

 

产品应用

建议稀释浓度:

IHC/ICC:1:500-1:5000;

ELISA/WB(显色法):1:5000-1:100000;

ELISA/WB(化学发光法):1:10000-1:200000;

 

产品信息

抗体浓度(Antibody Concentration)

100 μL(0.4 mg/mL)

缓冲液(Buffer)

0.005M 磷酸钠,0.125M 氯化钠,pH7.6

稳定剂(Stabilizer)

7.5 mg/mL BSA(无IgG,蛋白酶), 50%甘油

防腐剂(Preservative)

None(叠氮化钠会抑制HRP活性)

原料来源(Source of Material)

Jackson Immunoresearch 111-035-003

 

储存条件

-25 ~ -15℃分装保存,尽量避免反复冻融。有效期1年。

 

注意事项

1)本品不能保存于叠氮化钠中其会抑制辣根过氧化酶(HRP)活性;

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

3)本产品仅作科研用途!

 

过氧化物酶HRP标记山羊抗兔IgG抗体|Peroxidase-Conjugated Goat Anti-Rabbit IgG

暂无内容

[1] Wang Z, Lu Z, Lin S, et al. Leucine-tRNA-synthase-2-expressing B cells contribute to colorectal cancer immunoevasion. Immunity. 2022;55(6):1067-1081.e8. doi:10.1016/j.immuni.2022.04.017(IF:43.474)
[2] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)
[3] Yang WQ, Xiong QP, Ge JY, et al. THUMPD3-TRMT112 is a m2G methyltransferase working on a broad range of tRNA substrates. Nucleic Acids Res. 2021;49(20):11900-11919. doi:10.1093/nar/gkab927(IF:16.971)
[4] Qian Y, Liu Q, Li P, et al. Highly Tumor-Specific and Long-Acting Iodine-131 Microbeads for Enhanced Treatment of Hepatocellular Carcinoma with Low-Dose Radio-Chemoembolization. ACS Nano. 2021;15(2):2933-2946. doi:10.1021/acsnano.0c09122(IF:15.881)
[5] Wang K, Jiang L, Hu A, et al. Vertebral-specific activation of the CX3CL1/ICAM-1 signaling network mediates non-small-cell lung cancer spinal metastasis by engaging tumor cell-vertebral bone marrow endothelial cell interactions. Theranostics. 2021;11(10):4770-4789. Published 2021 Mar 4. doi:10.7150/thno.54235(IF:11.556)
[6] Han L, Lai H, Yang Y, et al. A 5'-tRNA halve, tiRNA-Gly promotes cell proliferation and migration via binding to RBM17 and inducing alternative splicing in papillary thyroid cancer. J Exp Clin Cancer Res. 2021;40(1):222. Published 2021 Jul 5. doi:10.1186/s13046-021-02024-3(IF:11.161)
[7] Gao JP, Xu P, Wang M, et al. Nod factor receptor complex phosphorylates GmGEF2 to stimulate ROP signaling during nodulation. Curr Biol. 2021;31(16):3538-3550.e5. doi:10.1016/j.cub.2021.06.011(IF:10.834)
[8] Wang T, Hu J, Luo H, et al. Photosensitizer and Autophagy Promoter Coloaded ROS-Responsive Dendrimer-Assembled Carrier for Synergistic Enhancement of Tumor Growth Suppression. Small. 2018;14(38):e1802337. doi:10.1002/smll.201802337(IF:9.598)
[9] Wu X, Qiao S, Wang W, et al. Melatonin prevents peri‑implantitis via suppression of TLR4/NF-κB. Acta Biomater. 2021;134:325-336. doi:10.1016/j.actbio.2021.07.017(IF:8.947)
[10] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 synergizes with PD-1 blockade through enhancing antitumor immunity in the tumor microenvironment. J Immunother Cancer. 2019;7(1):327. Published 2019 Nov 28. doi:10.1186/s40425-019-0750-6(IF:8.728)
[11] Song Y, Guo Y, Li X, et al. RBM39 Alters Phosphorylation of c-Jun and Binds to Viral RNA to Promote PRRSV Proliferation. Front Immunol. 2021;12:664417. Published 2021 May 17. doi:10.3389/fimmu.2021.664417(IF:7.561)
[12] Wang C, Qu L, Li S, et al. Discovery of First-in-Class Dual PARP and EZH2 Inhibitors for Triple-Negative Breast Cancer with Wild-Type BRCA. J Med Chem. 2021;64(17):12630-12650. doi:10.1021/acs.jmedchem.1c00567(IF:7.446)
[13] Song M, Wang Y, Chen Z, et al. Human CYP enzyme-activated genotoxicity of 2,2',4,4'-tetrabromobiphenyl ether in mammalian cells. Chemosphere. 2022;291(Pt 1):132784. doi:10.1016/j.chemosphere.2021.132784(IF:7.086)
[14] Liu S, Xu DS, Li M, et al. Icariin attenuates endothelial-mesenchymal transition via H19/miR-148b-3p/ELF5 in ox-LDL-stimulated HUVECs. Mol Ther Nucleic Acids. 2020;23:464-475. Published 2020 Dec 3. doi:10.1016/j.omtn.2020.11.021(IF:7.032)
[15] Wang C, Zhang H, Fu J, et al. Bartonella type IV secretion effector BepC induces stress fiber formation through activation of GEF-H1. PLoS Pathog. 2021;17(1):e1009065. Published 2021 Jan 28. doi:10.1371/journal.ppat.1009065(IF:6.823)
[16] Liu W, Guan Y, Qiao S, et al. Antiaging Effects of Vicatia thibetica de Boiss Root Extract on Caenorhabditis elegans and Doxorubicin-Induced Premature Aging in Adult Mice. Oxid Med Cell Longev. 2021;2021:9942090. Published 2021 Aug 6. doi:10.1155/2021/9942090(IF:6.543)
[17] Qu L, Li S, Ji L, et al. Discovery of PT-65 as a highly potent and selective Proteolysis-targeting chimera degrader of GSK3 for treating Alzheimer's disease. Eur J Med Chem. 2021;226:113889. doi:10.1016/j.ejmech.2021.113889(IF:6.514)
[18] Lao Y, Wang Y, Chen J, et al. Synthesis and biological evaluation of 1,2,4-triazole derivatives as potential Nrf2 activators for the treatment of cerebral ischemic injury. Eur J Med Chem. 2022;236:114315. doi:10.1016/j.ejmech.2022.114315(IF:6.514)
[19] Wang C, Chen X, Liu X, et al. Discovery of precision targeting EZH2 degraders for triple-negative breast cancer. Eur J Med Chem. 2022;238:114462. doi:10.1016/j.ejmech.2022.114462(IF:6.514)
[20] Qu L, Ji L, Wang C, et al. Synthesis and evaluation of multi-target-directed ligands with BACE-1 inhibitory and Nrf2 agonist activities as potential agents against Alzheimer's disease. Eur J Med Chem. 2021;219:113441. doi:10.1016/j.ejmech.2021.113441(IF:6.514)
[21] Bu S, Wang Q, Sun J, Li X, Gu T, Lai D. Melatonin suppresses chronic restraint stress-mediated metastasis of epithelial ovarian cancer via NE/AKT/β-catenin/SLUG axis [published correction appears in Cell Death Dis. 2020 Sep 8;11(9):726]. Cell Death Dis. 2020;11(8):644. Published 2020 Aug 18. doi:10.1038/s41419-020-02906-y(IF:6.304)
[22] Ning FL, Tao J, Li DD, et al. Activating BK channels ameliorates vascular smooth muscle calcification through Akt signaling. Acta Pharmacol Sin. 2022;43(3):624-633. doi:10.1038/s41401-021-00704-6(IF:6.150)
[23] Wang C, Zhang J, Song S, et al. Imaging epileptic foci in mouse models via a low-density lipoprotein receptor-related protein-1 targeting strategy. EBioMedicine. 2021;63:103156. doi:10.1016/j.ebiom.2020.103156(IF:5.736)
[24] Xu R, Lai C, Yang F, et al. Preliminary Characterization of Two Small Insulinase-Like Proteases in Cryptosporidium parvum. Front Microbiol. 2021;12:651512. Published 2021 May 21. doi:10.3389/fmicb.2021.651512(IF:5.640)
[25] Su J, Jin C, Wu H, et al. Differential Expression of Three Cryptosporidium Species-Specific MEDLE Proteins. Front Microbiol. 2019;10:1177. Published 2019 May 29. doi:10.3389/fmicb.2019.01177(IF:5.640)
[26] Zhang S, Wang Y, Wu H, et al. Characterization of a Species-Specific Insulinase-Like Protease in Cryptosporidium parvum. Front Microbiol. 2019;10:354. Published 2019 Mar 6. doi:10.3389/fmicb.2019.00354(IF:5.640)
[27] Yang R, Li D, Yi S, Wang M. Evolutionarily conserved odorant-binding proteins participate in establishing tritrophic interactions. iScience. 2022;25(7):104664. Published 2022 Jun 23. doi:10.1016/j.isci.2022.104664(IF:5.458)
[28] Fang DD, Tang Q, Kong Y, et al. MDM2 inhibitor APG-115 exerts potent antitumor activity and synergizes with standard-of-care agents in preclinical acute myeloid leukemia models. Cell Death Discov. 2021;7(1):90. Published 2021 May 3. doi:10.1038/s41420-021-00465-5(IF:5.241)
[29] Ma Y, Zheng K, Pang Y, et al. Anti-hypertrophic effect of synovium-derived stromal cells on costal chondrocytes promotes cartilage repairs. J Orthop Translat. 2021;32:59-68. Published 2021 Jun 2. doi:10.1016/j.jot.2021.05.002(IF:5.191)
[30] Wu X, Jin S, Yang Y, et al. Altered expression of ferroptosis markers and iron metabolism reveals a potential role of ferroptosis in vitiligo. Pigment Cell Melanoma Res. 2022;35(3):328-341. doi:10.1111/pcmr.13032(IF:4.693)
[31] Li Z, Liu S, Fu T, Peng Y, Zhang J. Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):351. Published 2019 Nov 27. doi:10.1186/s13287-019-1441-4(IF:4.627)
[32] Shi X, Ding K, Zhao Q, et al. Suppression of CPSF6 Enhances Apoptosis Through Alternative Polyadenylation-Mediated Shortening of the VHL 3'UTR in Gastric Cancer Cells. Front Genet. 2021;12:707644. Published 2021 Sep 14. doi:10.3389/fgene.2021.707644(IF:4.599)
[33] Chen Z, Li Z, Jiang C, Jiang X, Zhang J. MiR-92b-3p promotes neurite growth and functional recovery via the PTEN/AKT pathway in acute spinal cord injury. J Cell Physiol. 2019;234(12):23043-23052. doi:10.1002/jcp.28864(IF:4.522)
[34] Yao H, Xu K, Zhou J, Zhou L, Wei S. A Tumor Microenvironment Destroyer for Efficient Cancer Suppression. ACS Biomater Sci Eng. 2020;6(1):450-462. doi:10.1021/acsbiomaterials.9b01544(IF:4.511)
[35] Sun X, Zhang J, Nie Q. Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples. PLoS Comput Biol. 2021;17(3):e1008379. Published 2021 Mar 5. doi:10.1371/journal.pcbi.1008379(IF:4.475)
[36] Yin L, Zhang L, Luo L, et al. Berbamine reduces body weight via suppression of small GTPase Rab8a activity and activation of paraventricular hypothalamic neurons in obese mice. Eur J Pharmacol. 2022;916:174679. doi:10.1016/j.ejphar.2021.174679(IF:4.432)
[37] Fang DD, Zhu H, Tang Q, et al. FLT3 inhibition by olverembatinib (HQP1351) downregulates MCL-1 and synergizes with BCL-2 inhibitor lisaftoclax (APG-2575) in preclinical models of FLT3-ITD mutant acute myeloid leukemia. Transl Oncol. 2022;15(1):101244. doi:10.1016/j.tranon.2021.101244(IF:4.243)
[38] Li D, Qiu X, Yang J, Liu T, Luo Y, Lu Y. Generation of Human Lens Epithelial-Like Cells From Patient-Specific Induced Pluripotent Stem Cells. J Cell Physiol. 2016;231(12):2555-2562. doi:10.1002/jcp.25374(IF:4.155)
[39] Su J, Shen Y, Li N, et al. Comparative Characterization of CpCDPK1 and CpCDPK9, Two Potential Drug Targets Against Cryptosporidiosis. Microorganisms. 2022;10(2):333. Published 2022 Feb 1. doi:10.3390/microorganisms10020333(IF:4.128)
[40] Guo Y, Huo J, Wu D, et al. Simvastatin inhibits the adipogenesis of bone marrow‑derived mesenchymal stem cells through the downregulation of chemerin/CMKLR1 signaling. Int J Mol Med. 2020;46(2):751-761. doi:10.3892/ijmm.2020.4606(IF:4.101)
[41] Yang B, Li Y, Ma Y, et al. Selenium attenuates ischemia/reperfusion injury‑induced damage to the blood‑brain barrier in hyperglycemia through PI3K/AKT/mTOR pathway‑mediated autophagy inhibition. Int J Mol Med. 2021;48(3):178. doi:10.3892/ijmm.2021.5011(IF:4.101)
[42] Liu S, Xu DS, Ma JL, Huang P, Wu D, Ren LQ. LncRNA H19 Mitigates Oxidized Low-Density Lipoprotein Induced Pyroptosis via Caspase-1 in Raw 264.7 Cells. Inflammation. 2021;44(6):2407-2418. doi:10.1007/s10753-021-01511-1(IF:4.092)
[43] Zhang H, Guo F, Qi P, et al. OsHDA710-Mediated Histone Deacetylation Regulates Callus Formation of Rice Mature Embryo. Plant Cell Physiol. 2020;61(9):1646-1660. doi:10.1093/pcp/pcaa086(IF:4.062)
[44] Sun T, Zhang L, Feng J, et al. Characterization of cellular senescence in doxorubicin-induced aging mice. Exp Gerontol. 2022;163:111800. doi:10.1016/j.exger.2022.111800(IF:4.032)
[45] Wang J, Zhang Y, Liu X, Liu H. Is the Fixed Periodic Treatment Effective for the Tumor System without Complete Information?. Cancer Manag Res. 2021;13:8915-8928. Published 2021 Nov 30. doi:10.2147/CMAR.S339787(IF:3.989)
[46] Chen M, Lin H, Gao Y, Wang Z, Li Y, Jin F. Ghrelin attenuates drowning injury via dual effects on damage protection and immune repression. Ann Transl Med. 2021;9(11):920. doi:10.21037/atm-21-795(IF:3.932)
[47] Shi L, Zhang XB, Shi YF, et al. OsCDC48/48E complex is required for plant survival in rice (Oryza sativa L.). Plant Mol Biol. 2019;100(1-2):163-179. doi:10.1007/s11103-019-00851-9(IF:3.928)
[48] Shi J, Wang Y, Chen J, et al. Synthesis and biological evaluation of 1,2,4-oxadiazole core derivatives as potential neuroprotectants against acute ischemic stroke. Neurochem Int. 2021;148:105103. doi:10.1016/j.neuint.2021.105103(IF:3.921)
[49] Zhang KW, Wang D, Cai H, et al. IL‑6 plays a crucial role in epithelial‑mesenchymal transition and pro‑metastasis induced by sorafenib in liver cancer. Oncol Rep. 2021;45(3):1105-1117. doi:10.3892/or.2021.7926(IF:3.906)
[50] Qu F, Song Y, Wu Y, et al. The protective role of Ephrin-B2/EphB4 signaling in osteogenic differentiation under inflammatory environment. Exp Cell Res. 2021;400(2):112505. doi:10.1016/j.yexcr.2021.112505(IF:3.905)
[51] Cheng Q, Fan X, Liu Y, et al. miR-455-5p regulates circadian rhythms by accelerating the degradation of Clock mRNA. IUBMB Life. 2022;74(3):245-258. doi:10.1002/iub.2587(IF:3.885)
[52] He RZ, Zheng JH, Yao HF, et al. ADAMTS12 promotes migration and epithelial-mesenchymal transition and predicts poor prognosis for pancreatic cancer [published online ahead of print, 2022 Apr 25]. Hepatobiliary Pancreat Dis Int. 2022;S1499-3872(22)00117-5. doi:10.1016/j.hbpd.2022.04.005(IF:3.780)
[53] Wang Y, Wang H, Zhang L, et al. Potential mechanisms of tremor tolerance induced in rats by the repeated administration of total alkaloid extracts from the seeds of Peganum harmala Linn. J Ethnopharmacol. 2020;262:113183. doi:10.1016/j.jep.2020.113183(IF:3.690)
[54] Bu S, Li B, Wang Q, et al. Epithelial ovarian cancer stem‑like cells are resistant to the cellular lysis of cytokine‑induced killer cells via HIF1A‑mediated downregulation of ICAM‑1. Int J Oncol. 2019;55(1):179-190. doi:10.3892/ijo.2019.4794(IF:3.571)
[55] Shen Y, Zhou M, Yan J, et al. miR-200b inhibits TNF-α-induced IL-8 secretion and tight junction disruption of intestinal epithelial cells in vitro. Am J Physiol Gastrointest Liver Physiol. 2017;312(2):G123-G132. doi:10.1152/ajpgi.00316.2016(IF:3.468)
[56] Yao C, Chen X, Xu Y, et al. Comparing pretreatment strategies to increase the yield and purity of human urinary extracellular vesicles [published online ahead of print, 2022 Jun 30]. J Chromatogr B Analyt Technol Biomed Life Sci. 2022;1206:123359. doi:10.1016/j.jchromb.2022.123359(IF:3.318)
[57] Cao H, Hua D, Zhang H, et al. Oral immunization of recombinant Saccharomyces cerevisiae expressing fiber-2 of fowl adenovirus serotype 4 induces protective immunity against homologous infection. Vet Microbiol. 2022;271:109490. doi:10.1016/j.vetmic.2022.109490(IF:3.293)
[58] Li Q , Sun J , Mohammadtursun N , Wu J , Dong J , Li L . Curcumin inhibits cigarette smoke-induced inflammation via modulating the PPARγ-NF-κB signaling pathway. Food Funct. 2019;10(12):7983-7994. doi:10.1039/c9fo02159k(IF:3.241)
[59] Yang LY, Liu XF, Yang Y, et al. Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities. Acta Pharmacol Sin. 2017;38(1):56-68. doi:10.1038/aps.2016.89(IF:3.166)
[60] Fei J, Wu H, Su J, et al. Characterization of MEDLE-1, a protein in early development of Cryptosporidium parvum. Parasit Vectors. 2018;11(1):312. Published 2018 May 23. doi:10.1186/s13071-018-2889-2(IF:3.163)
[61] Yao Y, Luo R, Xiong S, Zhang C, Zhang Y. Protective effects of curcumin against rat intestinal inflammation‑related motility disorders. Mol Med Rep. 2021;23(5):391. doi:10.3892/mmr.2021.12030(IF:2.952)
[62] Li X, An P, Zhao Y, et al. Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells. Evid Based Complement Alternat Med. 2022;2022:3664696. Published 2022 Apr 22. doi:10.1155/2022/3664696(IF:2.630)
[63] Li D, Han X, Zhao Z, Lu Y, Yang J. Functional analysis of deleterious EPHA2 SNPs in lens epithelial cells. Mol Vis. 2021;27:384-395. Published 2021 Jun 23. (IF:2.367)
[64] You J, Cheng Y, Yang XJ, Chen L. Generation of a homozygous LRP2 knockout human embryonic stem cell line (FDCHDPe010-A-56) by CRISPR/Cas9 system. Stem Cell Res. 2021;53:102342. doi:10.1016/j.scr.2021.102342(IF:2.020)
[65] You J, Xi H, Ma S, Yang XJ, Chen L. Generation of a homozygous LRPAP1 knockout human embryonic stem cell line (FDCHDPe009-B) by CRISPR/Cas9 system. Stem Cell Res. 2021;56:102516. doi:10.1016/j.scr.2021.102516(IF:2.020)
[66] Zhu T, Zhao Y, Wu Y, Qu D. The Staphylococcus epidermidis gdpS regulates biofilm formation independently of its protein-coding function. Microb Pathog. 2017;105:264-271. doi:10.1016/j.micpath.2017.02.045(IF:2.009)
[67] Liu X, Xie F, Lai G, Wang J. Roles of heterogeneous nuclear ribonucleoprotein L in enamel organ development and the differentiation of ameloblasts. Arch Oral Biol. 2020;120:104933. doi:10.1016/j.archoralbio.2020.104933(IF:1.931)
[68] Jiang J, Chen Z, Yang Y, Yan J, Jiang H. Sevoflurane downregulates IGF‑1 via microRNA‑98. Mol Med Rep. 2017;15(4):1863-1868. doi:10.3892/mmr.2017.6219(IF:1.692)
[69] Xia Q, Zhou Y, Wang X, Fu S. Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis. Exp Ther Med. 2018;15(2):1586-1593. doi:10.3892/etm.2017.5549(IF:1.261)
[70] Li X, Lei Y, Yu Y, et al. Discovery and characterization of a novel splice variant of the p53 tumor suppressor gene in a human T cell leukemia cellline. Int J Clin Exp Pathol. 2020;13(5):1121-1135. Published 2020 May 1. (IF:0.252)
[71] Feng Y, Jiang C, Yang F, Chen Z, Li Z. Apocynum venetum leaf extract protects against H2O2-induced oxidative stress by increasing autophagy in PC12 cells. Biomed Rep. 2020;13(2):6. doi:10.3892/br.2020.1313(IF:0.000)

HRP标记小鼠抗HA-Tag单克隆抗体|HA-Tag,Mouse mAb(HRP)

HRP标记小鼠抗HA-Tag单克隆抗体|HA-Tag,Mouse mAb(HRP)

产品说明书

FAQ

COA

已发表文献

产品信息
 

产品名称

产品编号

规格

储存条件

价格(元)

HRP-conjugated HA-Tag, Mouse mAb小鼠抗HA-Tag单克隆抗体,HRP标记

30703ES20

20μl (>50次)

-20 ºC

418.00

HRP-conjugated HA-Tag, Mouse mAb小鼠抗HA-Tag单克隆抗体,HRP标记

30703ES60

100μl(>250次)

-20 ºC

988.00

产品描述
 

      人流感病毒血凝素(Human influenza hemagglutinin, HA)是决定人病毒传染性的一类表面糖蛋白。HA标签(HA-Tag)来源于HA分子对应的98-106位氨基酸(YPYDVPDYA ),广泛用作表达载体的通用表位标签。许多融合蛋白经基因工程重组后表达HA tag,且融合蛋白本身的生物活性和体内分布并不会受影响。其他常用的标签还包括myc、Flag、His、GST等,标签的使用简化了蛋白的检测,分离和纯化。

      本抗体特异性识别HA Tag,不仅利于检测带HA Tag的融合蛋白的表达量,另外还利于后续对融合蛋白的分离纯化。
 

产品类型
 

Mouse ascitesmonoclonal antibody (Mouse mAb)
 

产品介绍
 

反应性(Specificity

All

宿主(Host

Mouse

应用(Application

WB:1:1000~1:10000

缓冲液(Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, pH 7.2

纯化(Purified

亲和纯化; Purity:>95%(SDS-PAGE)
 

运输与保存方法
 

冰袋(wet ice)运输。

短期保存于4℃,长期请于-20℃分装冻存,避免反复冻融。
 

注意事项
 

1)使用前先离心,以免造成不必要的损失。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。
3)本产品仅作科研用途!

 

相关产品
 

30701ES20

HA-Tag, Mouse mAb小鼠抗HA-Tag单克隆抗体

20μl (>50次)

30701ES60

HA-Tag, Mouse mAb小鼠抗HA-Tag单克隆抗体

100μl (>250次)

30702ES20

HA-Tag, Rabbit pAb兔抗HA-Tag多克隆抗体

20μl (>50次)

30702ES60

HA-Tag, Rabbit pAb兔抗HA-Tag多克隆抗体

100μl (>250次)

 

 

 

HRP标记小鼠抗HA-Tag单克隆抗体|HA-Tag,Mouse mAb(HRP)

暂无内容

HRP标记小鼠抗HA-Tag单克隆抗体|HA-Tag,Mouse mAb(HRP)

暂无内容

产品信息
 

产品名称

产品编号

规格

储存条件

价格(元)

HRP-conjugated HA-Tag, Mouse mAb小鼠抗HA-Tag单克隆抗体,HRP标记

30703ES20

20μl (>50次)

-20 ºC

418.00

HRP-conjugated HA-Tag, Mouse mAb小鼠抗HA-Tag单克隆抗体,HRP标记

30703ES60

100μl(>250次)

-20 ºC

988.00

产品描述
 

      人流感病毒血凝素(Human influenza hemagglutinin, HA)是决定人病毒传染性的一类表面糖蛋白。HA标签(HA-Tag)来源于HA分子对应的98-106位氨基酸(YPYDVPDYA ),广泛用作表达载体的通用表位标签。许多融合蛋白经基因工程重组后表达HA tag,且融合蛋白本身的生物活性和体内分布并不会受影响。其他常用的标签还包括myc、Flag、His、GST等,标签的使用简化了蛋白的检测,分离和纯化。

      本抗体特异性识别HA Tag,不仅利于检测带HA Tag的融合蛋白的表达量,另外还利于后续对融合蛋白的分离纯化。
 

产品类型
 

Mouse ascitesmonoclonal antibody (Mouse mAb)
 

产品介绍
 

反应性(Specificity

All

宿主(Host

Mouse

应用(Application

WB:1:1000~1:10000

缓冲液(Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, pH 7.2

纯化(Purified

亲和纯化; Purity:>95%(SDS-PAGE)
 

运输与保存方法
 

冰袋(wet ice)运输。

短期保存于4℃,长期请于-20℃分装冻存,避免反复冻融。
 

注意事项
 

1)使用前先离心,以免造成不必要的损失。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。
3)本产品仅作科研用途!

 

相关产品
 

30701ES20

HA-Tag, Mouse mAb小鼠抗HA-Tag单克隆抗体

20μl (>50次)

30701ES60

HA-Tag, Mouse mAb小鼠抗HA-Tag单克隆抗体

100μl (>250次)

30702ES20

HA-Tag, Rabbit pAb兔抗HA-Tag多克隆抗体

20μl (>50次)

30702ES60

HA-Tag, Rabbit pAb兔抗HA-Tag多克隆抗体

100μl (>250次)

 

 

 

HRP标记小鼠抗HA-Tag单克隆抗体|HA-Tag,Mouse mAb(HRP)

暂无内容

HRP标记小鼠抗HA-Tag单克隆抗体|HA-Tag,Mouse mAb(HRP)

暂无内容

fabgennix HRP-1060说明书

fabgennix HRP-1060说明书

兔抗鸡 IgG HRP

产品编号:HRP-1060

一般信息

 

产品

 

抗鸡 IgY HRP

描述

兔抗鸡 IgY(重链和轻链)-马萝卜过氧化物酶

已验证的应用程序

CM、ELISA、IF、IHC、IP、WB

主持人

兔子

物种交叉反应

免疫原

鸡IgY全分子

物理性质

 

体积

 

500 微升

专注

.5 µg/µl 抗体稳定缓冲液

形式

液体

免疫球蛋白

IgY 重链和轻链

克隆性

多克隆

贮存

储存在 -20⁰C。从 -20⁰C 中取出后,无需解冻即可立即将偶联物用于移液。 

存储缓冲区

抗血清在含有 0.02%硫柳汞或硫柳汞作为防腐剂的抗体稳定缓冲液中提供。

应用协议

 

共聚焦显微镜

 

1:250-1:5,000

D B

1:30,000-1:75,000

酶联免疫吸附试验

1:30,000-1:75,000

免疫细胞化学

1:250-1:5,000

免疫荧光

1:250-1:5,000

免疫组化

1:250-1:5,000

免疫沉淀

1:250-1:5,000

蛋白质印迹

1:4,500-1:2 0,000

 

小鼠抗人IgG4 Fc-HRP单克隆抗体 说明书

货号:9200-05

产品描述:Mouse Anti-Human IgG4 Fc-HRP (小鼠抗人IgG4 Fc-HRP单克隆抗体

规格:1.0ml

品牌:SouthernBiotech 

Southernbiotech专门致力于开发、生产、净化、共轭、和商品化世界上zui高质量的抗体研究使用。

 

克隆

HP6025

同型

小鼠(BALB / c)中的IgG 1 K

同种型对照

小鼠IgG 1 -HRP

免疫原

人IgG 4骨髓瘤蛋白质

特异性

人IgG 4 Fc

格式/共轭

HRP(辣根过氧化物酶)

缓冲液配方

50%甘油/ 50%PBS(pH 7.4); 没有添加防腐剂

规格

1.0毫升

存储和处理

2-8°C储存

应用

ELISA – 质量检测

FLISA – 质量检测
流式细胞仪

免疫组化 – 冷冻切片

免疫组化 – 石蜡切片

免疫细胞化学

蛋白质印迹 

微阵列

Multiplex

纯化 

ELISA板用纯化的人IgG1,IgG2,IgG3和IgG4包被。用连续稀释的小鼠抗人IgG4 Fc-HRP(SB目录号9200-05)检测免疫球蛋白。

产品链接:https://www.southernbiotech.com/?catno=9200-05&type=Monoclonal

 

小鼠抗GST-tag单克隆抗体 HRP标记小鼠单克隆抗体|GST-tag,Mouse mAb(HRP)

小鼠抗GST-tag单克隆抗体 HRP标记小鼠单克隆抗体|GST-tag,Mouse mAb(HRP)

产品说明书

FAQ

COA

已发表文献

产品描述

融合蛋白表达中通常在蛋白N-端或C-端加上特定蛋白或多肽序列作为一种抗原表位(标签蛋白)以便于目的蛋白后续的定位、表达、检测及纯化。Glutathione S-Transferase (即谷胱甘肽S-转移酶,GST)是应用非常广泛的一种蛋白标签,来源于Schistosoma japonicum,分子量为26kDa,可应用于各种融合蛋白,可在大肠杆菌和酵母菌等宿主细胞中表达,用GST亲和柱可简便地分离融合蛋白。 GST标签有助于保护重组蛋白免受胞外蛋白酶的降解并提高其稳定性。

GST抗体可以用于GST融合蛋白后续的表达、细胞内定位,纯化、以及定性或定量分析。

 

产品介绍

产品类型(Isotype) 小鼠单抗(mouse McAb  IgG2a)
分子量(Molecular Weight) 26 kDa
反应性(Specificity) Recombinant protein, Schistosoma japonicum
应用(Application) WB : 1:5000-1:50000
免疫原(Immunogen) Recombinant Protein
纯化方式 Protein A purification
储存缓冲液(Storage Buffer) PBS with 0.02% thimerosal and 50% glycerol pH 7.3。

 

运输与保存方法
冰袋(wet ice)运输。-20℃保存,避免反复冻融。

注意事项

1)使用前请先离心,以免造成不必要的损失。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

3)本产品仅作科研用途!

小鼠抗GST-tag单克隆抗体 HRP标记小鼠单克隆抗体|GST-tag,Mouse mAb(HRP)

暂无内容

小鼠抗GST-tag单克隆抗体 HRP标记小鼠单克隆抗体|GST-tag,Mouse mAb(HRP)

暂无内容

产品描述

融合蛋白表达中通常在蛋白N-端或C-端加上特定蛋白或多肽序列作为一种抗原表位(标签蛋白)以便于目的蛋白后续的定位、表达、检测及纯化。Glutathione S-Transferase (即谷胱甘肽S-转移酶,GST)是应用非常广泛的一种蛋白标签,来源于Schistosoma japonicum,分子量为26kDa,可应用于各种融合蛋白,可在大肠杆菌和酵母菌等宿主细胞中表达,用GST亲和柱可简便地分离融合蛋白。 GST标签有助于保护重组蛋白免受胞外蛋白酶的降解并提高其稳定性。

GST抗体可以用于GST融合蛋白后续的表达、细胞内定位,纯化、以及定性或定量分析。

 

产品介绍

产品类型(Isotype) 小鼠单抗(mouse McAb  IgG2a)
分子量(Molecular Weight) 26 kDa
反应性(Specificity) Recombinant protein, Schistosoma japonicum
应用(Application) WB : 1:5000-1:50000
免疫原(Immunogen) Recombinant Protein
纯化方式 Protein A purification
储存缓冲液(Storage Buffer) PBS with 0.02% thimerosal and 50% glycerol pH 7.3。

 

运输与保存方法
冰袋(wet ice)运输。-20℃保存,避免反复冻融。

注意事项

1)使用前请先离心,以免造成不必要的损失。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

3)本产品仅作科研用途!

小鼠抗GST-tag单克隆抗体 HRP标记小鼠单克隆抗体|GST-tag,Mouse mAb(HRP)

暂无内容

小鼠抗GST-tag单克隆抗体 HRP标记小鼠单克隆抗体|GST-tag,Mouse mAb(HRP)

暂无内容

小鼠抗His-tag单克隆抗体(HRP标记)|His-tag Mouse mAb(HRP)

小鼠抗His-tag单克隆抗体(HRP标记)|His-tag Mouse mAb(HRP)

产品说明书

FAQ

COA

已发表文献

产品简介

 

His标签是由6个组氨酸(His-His-His-His-His-His)组成的短肽,可与多种蛋白形成融合蛋白,基于组氨酸的咪唑环与金属离子(如镍离子)起化学作用而生成螯合的原理,可以利用His-Tag标签纯化His融合蛋白。由于分子量较小,并且较容易分离和纯化,His融合标签与其他标签相比有很多明显优势,是目前融合标签中使用最为广泛的一种。

本品为HRP标记的His-tag抗体,可特异性识别来源于多种物种(如细菌、昆虫、哺乳动物)的位于N端或C端His的融合蛋白。抗体具有高灵敏度,高特异性和高亲和力。

 

产品应用

 

建议稀释浓度:WB:1:1000-1:10000;

 

产品信息

 

产品类型(Product Type)

Mouse mAb IgG1

克隆号(Clone No.)

9C11

纯化方式(Purification method)

Affinity Purified

免疫原(Immunogen)

偶联KLH的6×His多肽

应用(Application)

WB

储存缓冲液(Storage Buffer)

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

 

储存条件

 

-25 ~ -15℃分装保存,尽量避免反复冻融。

 

注意事项

 

1.使用前先离心,以免造成不必要的损失。

2.为了您的安全和健康,请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途!

Ver.CN20230802

小鼠抗His-tag单克隆抗体(HRP标记)|His-tag Mouse mAb(HRP)

暂无内容

[1] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)
[2] Tan FQ, Wang W, Li J, et al. A coiled-coil protein associates Polycomb Repressive Complex 2 with KNOX/BELL transcription factors to maintain silencing of cell differentiation-promoting genes in the shoot apex [published online ahead of print, 2022 May 4]. Plant Cell. 2022;koac133. doi:10.1093/plcell/koac133(IF:11.277)
[3] Zhang F, Chen Y, Ke Y, et al. Single Chain Fragment Variable (scFv) Antibodies Targeting the Spike Protein of Porcine Epidemic Diarrhea Virus Provide Protection against Viral Infection in Piglets. Viruses. 2019;11(1):58. Published 2019 Jan 14. doi:10.3390/v11010058(IF:3.811)

产品简介

 

His标签是由6个组氨酸(His-His-His-His-His-His)组成的短肽,可与多种蛋白形成融合蛋白,基于组氨酸的咪唑环与金属离子(如镍离子)起化学作用而生成螯合的原理,可以利用His-Tag标签纯化His融合蛋白。由于分子量较小,并且较容易分离和纯化,His融合标签与其他标签相比有很多明显优势,是目前融合标签中使用最为广泛的一种。

本品为HRP标记的His-tag抗体,可特异性识别来源于多种物种(如细菌、昆虫、哺乳动物)的位于N端或C端His的融合蛋白。抗体具有高灵敏度,高特异性和高亲和力。

 

产品应用

 

建议稀释浓度:WB:1:1000-1:10000;

 

产品信息

 

产品类型(Product Type)

Mouse mAb IgG1

克隆号(Clone No.)

9C11

纯化方式(Purification method)

Affinity Purified

免疫原(Immunogen)

偶联KLH的6×His多肽

应用(Application)

WB

储存缓冲液(Storage Buffer)

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

 

储存条件

 

-25 ~ -15℃分装保存,尽量避免反复冻融。

 

注意事项

 

1.使用前先离心,以免造成不必要的损失。

2.为了您的安全和健康,请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途!

Ver.CN20230802

小鼠抗His-tag单克隆抗体(HRP标记)|His-tag Mouse mAb(HRP)

暂无内容

[1] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)
[2] Tan FQ, Wang W, Li J, et al. A coiled-coil protein associates Polycomb Repressive Complex 2 with KNOX/BELL transcription factors to maintain silencing of cell differentiation-promoting genes in the shoot apex [published online ahead of print, 2022 May 4]. Plant Cell. 2022;koac133. doi:10.1093/plcell/koac133(IF:11.277)
[3] Zhang F, Chen Y, Ke Y, et al. Single Chain Fragment Variable (scFv) Antibodies Targeting the Spike Protein of Porcine Epidemic Diarrhea Virus Provide Protection against Viral Infection in Piglets. Viruses. 2019;11(1):58. Published 2019 Jan 14. doi:10.3390/v11010058(IF:3.811)

小鼠抗GFP-tag单克隆抗体 HRP标记小鼠单克隆抗体|GFP-tag,Mouse mAb(HRP)

小鼠抗GFP-tag单克隆抗体 HRP标记小鼠单克隆抗体|GFP-tag,Mouse mAb(HRP)

产品说明书

FAQ

COA

已发表文献

产品描述

GFPGreen Fluorescent Protein)最早是从一种生活在北太平洋寒冷水域的水母(Aequoria victoria)体内发现的蛋白,该蛋白在蓝色波长范围的光激发下能发出绿色的荧光,其最大和次大的激发波长是 395nm 470nm;最大发射波长是 509nm,在 540nm 处有一肩峰。

由于 GFP 具有荧光性质稳定,对细胞无毒害,表达具有光谱性等一系列的优点,常作为报告基因,融合标签和细胞标记物等,应用于分子和细胞生物学领域。

产品性质

别名(Synonym

CFPeGFPeYFPGFPGFP taqYFP

产品类型(Isotype

小鼠单克隆抗体(mouse ,pAbIgG

反应性(Reactivity

GFP 标签重组蛋白

宿主(Host

Mouse

应用(Application 

ELISAIFWBIP

抗原分子量(Antigen Molecular Weight

26kDa

储存缓冲液(Storage Buffer)

0.1%叠氮钠、50%甘油的 PBSpH7.3

纯化方式(Purification Method

Protein A 纯化

 

使用方法

推荐稀释倍数WB: 1:5000-1:50000

 

运输与保存方法

冰袋(wet ice)运输。-20℃保存,避免反复冻融,一年有效期。

注意事项

1)使用前先离心,以免造成不必要的损失。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作

3)本产品仅作科研用途

HB220627

小鼠抗GFP-tag单克隆抗体 HRP标记小鼠单克隆抗体|GFP-tag,Mouse mAb(HRP)

暂无内容

小鼠抗GFP-tag单克隆抗体 HRP标记小鼠单克隆抗体|GFP-tag,Mouse mAb(HRP)

暂无内容

产品描述

GFPGreen Fluorescent Protein)最早是从一种生活在北太平洋寒冷水域的水母(Aequoria victoria)体内发现的蛋白,该蛋白在蓝色波长范围的光激发下能发出绿色的荧光,其最大和次大的激发波长是 395nm 470nm;最大发射波长是 509nm,在 540nm 处有一肩峰。

由于 GFP 具有荧光性质稳定,对细胞无毒害,表达具有光谱性等一系列的优点,常作为报告基因,融合标签和细胞标记物等,应用于分子和细胞生物学领域。

产品性质

别名(Synonym

CFPeGFPeYFPGFPGFP taqYFP

产品类型(Isotype

小鼠单克隆抗体(mouse ,pAbIgG

反应性(Reactivity

GFP 标签重组蛋白

宿主(Host

Mouse

应用(Application 

ELISAIFWBIP

抗原分子量(Antigen Molecular Weight

26kDa

储存缓冲液(Storage Buffer)

0.1%叠氮钠、50%甘油的 PBSpH7.3

纯化方式(Purification Method

Protein A 纯化

 

使用方法

推荐稀释倍数WB: 1:5000-1:50000

 

运输与保存方法

冰袋(wet ice)运输。-20℃保存,避免反复冻融,一年有效期。

注意事项

1)使用前先离心,以免造成不必要的损失。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作

3)本产品仅作科研用途

HB220627

小鼠抗GFP-tag单克隆抗体 HRP标记小鼠单克隆抗体|GFP-tag,Mouse mAb(HRP)

暂无内容

小鼠抗GFP-tag单克隆抗体 HRP标记小鼠单克隆抗体|GFP-tag,Mouse mAb(HRP)

暂无内容

HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064)

HRP Tag Rabbit Monoclonal Antibody
HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064) 一键复制产品信息

产品编号: AG8064

产品包装:50μl
选择包装

50μl

说明书下载

456.00 10

价格: ¥ 456.00

促销价: ¥ 456.00

促销价: ¥ 456.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
AG8064 HRP Tag Rabbit Monoclonal Antibody 50μl 456.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Rabbit WB, FC, IF/ICC All 39kDa or Tagged protein 39kDa or Tagged protein

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; Ar, Arabidopsis; B, Bovine; C, Chicken; Cerastium, Ce; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Ph, Pharbitis; Pg, Pig; Rb, Rabbit; Rc, Rice; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:1,000-1:2,000 1:50-1:100 1:50-1:100 1:50-1:100

抗体详细信息如下:

About this Antibody
Name HRP Tag Rabbit Monoclonal Antibody
Category Recombinant Rabbit Monoclonal Antibody (mAb); Primary antibody; 标签抗体
Isotype IgG
Purification method Protein A affinity purification
Positive samples
Cellular location
Customer validation
About the Immunogen
Immunogen Full length native protein (purified) corresponding to HRP (Horseradish).
Sequence
Gene ID
SwissProt P00433
Synonyms PER1A_ARMRU, PRXC1A, HPRC1, Peroxidase C1A
Category
Background Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidativestress. These functions might be dependent on eachisozyme/isoform in each plant tissue.

包装清单:

产品编号 产品名称 包装
AG8064 HRP Tag Rabbit Monoclonal Antibody 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份

保存条件:

HRP Tag Rabbit Monoclonal Antibody -20℃保存,需避免反复冻融。Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000×g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
请根据抗体的实际用途选择相应的使用方法。
1.Western检测:
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的一抗,4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片。

HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064) All lanes: Anti-HRP antibody at 1:2,000 dilution Predicted MW: 39 kDa Observed MW: 39 kDa Lane 1: HRP protein Lysate at 20 ng per lane 2nd Ab: GAR HRP(H+L) 1:5,000 Exposure: 60s
HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064) HRP-tag Rabbit Monoclonal Antibody staining HRP in HeLa cells by IF/ICC . Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with anti-actin antibody (1:200) conjugated to HRP at 4°C. FITC-conjugated HRP-tag Rabbit Monoclonal Antibody was used as the secondary antibody (1:50). DAPI (blue) was used as the nuclear counter stain. Control: primary antibody, anti-actin antibody (1:200) and PBS.
HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064) Overlay histogram showing HeLa cells stained with HRP-tag Rabbit Monoclonal Antibody (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% Triton X-100 for 15 min. The cells were then incubated in the antibody (Alpha smooth muscle actin Rabbit Monoclonal Antibody, 1:50 dilution) conjugated to HRP in 1x PBS/1% BSA for 60 min at room temperature. The secondary antibody used was HRP-tag Rabbit Monoclonal Antibody conjugated with FITC at 1:50 dilution for 60 min at room temperature. Unlabelled sample (Black) was used as a control.
HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064)
HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064)

相关产品:

产品编号 产品名称 包装
E6001 MiniProGel™蛋白制胶与电泳系统(4胶) 1套
E6005 MiniProGel™蛋白制胶与电泳系统(2胶) 1套
E6050 MiniBlot™蛋白转膜系统 1套
E6150 MiniProGel™蛋白制胶、电泳与转膜系统(2胶/2膜) 1套
E6155 MiniProGel™蛋白制胶、电泳与转膜系统(4胶/2膜) 1套
E6159 MiniProGel™蛋白制胶、电泳与转膜系统(4胶/4膜) 1套
E6080 BeyoPower™中电流电源(300V/600mA/100W) 1套
E6085 BeyoPower™高电流电源(300V/2000mA/200W) 1套
E6600 TissueMaster™手持式组织研磨仪 1套
EI600 BeyoImager™ 600化学发光成像系统 1套
FFP24 PVDF膜(进口分装, 6.6×8.5cm, 0.2μm) 20张/包装
FFP32 PVDF膜(进口分装, 6.6×8.5cm, 0.45μm) 20张/包装
FFP51 转印滤纸(7.5×10cm) 100张/包装
FFX050-FFX055 Western洗膜盒 1个/包装
P0006 Bradford蛋白浓度测定试剂盒 1000次
P0006C Bradford蛋白浓度测定试剂盒(去垢剂兼容型) 800次
P0012 BCA蛋白浓度测定试剂盒 500次
P0012A SDS-PAGE凝胶配制试剂盒 可制30-50块胶
P0012AC SDS-PAGE凝胶快速配制试剂盒 可制30-50块胶
P0013 Western及IP细胞裂解液 100ml
P0013B-P0013D RIPA裂解液(强、中、弱) 100ml
P0014A/P0014B SDS-PAGE电泳液(Tris-Gly, Powder) 1L/10L
P0014C/P0014D SDS-PAGE电泳液(Tris-Gly, 10X) 100ml/500ml
P0015/P0015L SDS-PAGE蛋白上样缓冲液(5X) 2ml/15ml
P0015F SDS-PAGE蛋白上样缓冲液(6X) 2ml
P0017F BeyoBlue™考马斯亮蓝超快染色液 250ml
P0018A BeyoECL Star (特超敏ECL化学发光试剂盒) 100ml/500ml
P0018F BeyoECL Moon (极超敏ECL化学发光试剂盒) 100ml/500ml
P0021A Western转膜液 1L
P0021S PVDF膜浸润活化液 250ml
P0022 丽春红染色液 120ml
P0023A Western一抗稀释液 100ml/500ml
P0023B Western封闭液 100ml/500ml
P0023C Western洗涤液 250ml
P0023D Western二抗稀释液 100ml/500ml
P0025 Western一抗二抗去除液 250ml
P0059 Recombinant 16-Tag Protein 200μl/1ml
P0066 预染蛋白质分子量标准(19-117kD) 200μl
P0068 彩色预染蛋白质分子量标准(10-180kD) 200μl
P0071 BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) 200μl
P0075 BeyoColor™彩色预染蛋白分子量标准(10-170kD) 200μl
P0078 BeyoColor™彩色预染蛋白分子量标准(15-120kD) 200μl
P0095 免疫染色通透液(Saponin) 100ml/500ml
P0096 免疫染色通透液(Triton X-100) 100ml/500ml
P0097 免疫染色强力通透液 100ml/500ml
P0098 免疫染色固定液 100ml/500ml
P0099 4%多聚甲醛固定液 100ml/500ml
P0102 免疫染色封闭液 100ml
P0103 免疫染色一抗稀释液 100ml
P0106 免疫染色洗涤液 250ml
P0108 免疫荧光染色二抗稀释液 100ml
P0220-P0235 Quickblock™封闭液 100ml
P0252 QuickBlock™ Western封闭液 100ml/500ml
P0256 QuickBlock™ Western一抗稀释液 100ml/500ml
P0258 QuickBlock™ Western二抗稀释液 100ml/500ml
P0260 QuickBlock™免疫染色封闭液 100ml
P0262 QuickBlock™免疫染色一抗稀释液 100ml
P0265 QuickBlock™免疫荧光染色二抗稀释液 100ml
P0267 QuickBlock™免疫组化染色二抗稀释液 100ml
P0280 InstantView™ SDS-PAGE蛋白染色及上样缓冲液(5X) 1ml
P0050-P0058 BeyoGel™ SDS-PAGE预制胶(Tris-Gly, 12孔) 10块/50块
P0448-P0472 BeyoGel™ Plus PAGE预制胶(Tris-Gly, 10孔/15孔) 10块/50块
P0501-P0528 BeyoGel™ Plus PAGE预制胶(Hepes, 10孔/15孔) 10块/50块
P0552 BeyoGel™ Plus SDS-PAGE Hepes电泳液(20X) 100ml/500ml
P0670-P0678 SDS-PAGE下层胶预混液 250ml/500ml
P0683 SDS-PAGE上层胶预混液 250ml/500ml
P0686-P0695 SDS-PAGE凝胶超快速配制试剂盒 可制30-50块胶
P1005-P1031 蛋白酶抑制剂混合物(50X/100X) 1ml/5ml
P1045-P1066 蛋白酶磷酸酶抑制剂混合物(50X) 1ml/2ml/5ml/10ml
P1081-P1097 磷酸酶抑制剂混合物(50X) 2ml/10ml
P1112/P1113 去乙酰化酶抑制剂混合物(100X) 1ml/5ml
R0121 AllProtect™动物组织核酸、蛋白稳定保存液 25ml/100ml

HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064)
HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064)

物质安全数据单
输入右侧验证码点击下载:

HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064)

HRP Tag Rabbit mAb(HRP Tag兔单抗)(AG8064)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
标签抗体
标签抗体

使用本产品的文献:

Proteintech HRP-66005 现货

世界*实验材料供应商 Proteintech Group,Inc.上海金畔生物为其中国代理, Proteintech Group,Inc.在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Proteintech Group,Inc.就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

 Proteintech Group,Inc.中国代理, Proteintech Group,Inc.上海代理, Proteintech Group,Inc.北京代理,Proteintech Group,Inc.广东代理, Proteintech Group,Inc.江苏代理Proteintech Group,Inc.湖北代理,Proteintech Group,Inc.天津,Proteintech Group,Inc.黑龙江代理,Proteintech Group,Inc.内蒙古代理,Proteintech Group,Inc.吉林代理,Proteintech Group,Inc.福建代理, Proteintech Group,Inc.江苏代理,Proteintech Group,Inc.浙江代理, Proteintech Group,Inc.四川代理,

 

Proteintech Group,Inc.于2001年在美国芝加哥成立,是一家专门从事研发、生产和销售抗体及重组蛋白的生物公司。针对生物前沿的热点研究,为科研工作者提 供新颖*的一抗及相关产品。武汉三鹰生物技术有限公司是Proteintech Group,Inc.在中国成立的全资子公司。

公司自创建以来,一贯以感受现代生物学发展的脉搏,把握当今生物学先进的技术,为生物学研究提供所需的抗 体、蛋白及相关产品,为生物学界同仁提供优良服务为己任。公司拥有*管理体系,确立了一整套生物科技企业的良性运营模式,建立起了坚实而广泛的欧美 以及国内市场销售体系,产品和服务已为国内外*实验室所接纳和采用,在当今方兴未艾的人类蛋白质组计划已占据了一席之地。

Proteintech建立了拥有22,000条人类基因的cDNA文库,10,000多种人类蛋白质抗体库。公司以强大的研发和技术能力,面向国内外所 有的生物实验室提供优良的技术服务,以及从克隆到大规模表达和抗体制备的整套。

 

HRP-Conjugated 6*His, His-Tag Antibody

 

货号: HRP-66005

小鼠单克隆

将重组蛋白进行SDS PAGE,随后用HRP-66005(6 * His,His-Tag抗体)以1:100稀释的蛋白质印迹

物种特异性:  重组蛋白,人

正面WB检测:  重组蛋白

推荐稀释度   WB:1:5000-1:50000

 

产品信息

资源: 老鼠

净化方法: 蛋白G纯化

同种型:   IgG1

存储:    PBS含0.02%硫柳汞,50%甘油,pH 7.3。储存于-20 Ô C.等分是不必要的-20 ö下储         存。

 

免疫原信息

免疫原:  6xHis肽

全名:   6 *他,他的标签

计算分子量:  1 kDa

观察分子量:  

GenBank登录号:

基因ID(NCBI): 

基因符号

同义词   6 * His,6 * His,His Tag,6histag,6XHis,HHHHHH,His tag,HISTIDINE TAGGED,HRP 6XHis,HRP HHHHHH,HRP His tag

 

货号 产品名称 规格型号 品牌
HRP-66005 HRP-conjugated 6*His, His-Tag Antibody 100ul Proteintech

Proteintech HRP-66005 HRP-conjugated 6*His, His-Tag Antibody 现货供应