世界*实验材料供应商 Cell Technology上海金畔生物为其中国代理， Cell Technology在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， Cell Technology就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
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Cell Technology成立于1998年，致力于为研究院、生物技术公司及制药企业的科研人员研发生产*的细胞功能研究用试剂与试剂盒。这些检测试剂与试剂盒可以实现不破坏细胞形态和结构的情况下原位检测细胞成分，研究细胞内发生的特定事件.Cell Technology发布了通过测定胞内活性caspase及线粒体膜电位研究细胞凋亡的一系列试剂。随着公司研发团队的持续努力，我们又推出了一系列高特异性的caspase检测试剂，以及cathepsin检测的试剂和ELISA试剂盒。目前Cell Technology提供的检测试剂和ELISA试剂盒涵盖Caspase、NOS、ROS、线粒体膜电位、信号通路和蛋白磷酸化等多个研究领域，产品倍各大高校、研究机构、生物技术企业和制药企业采用。
Cell Technology主要产品简介
1、Fluoro Cholesterol：高灵敏、一步检测生物样本中总胆固醇含量，可用于细胞代谢与药物作用的研究。
2、Fluoro NADP：检测组织或细胞提取液内NADP/NADPH含量，与NAD/NADH检测无交叉，可用于细胞死亡、抗氧化/氧化应激、能量代谢及线粒体功能研究。
3、Fluoro Sarcosine：定量检测细胞、血清及组织提取物中的肌氨酸含量。
4、Fluoro IndoBlu：定量检测细胞中IndoBlu的含量，适用于细胞增殖研究。
5、Fluoro Lactate：定量检测生物样本中 L-lactate的含量。
6、Fluoro Phosphate：定量检测生物样本中的磷含量，灵敏度可达200nM。
- 产品编号:FLNADH 100-2
- 产品名称:Fluoro NAD™ Fluorescent NAD/NADH Detection Kit – Detection of NAD/NADH in tissue extracts /cell samples/荧光NAD/NADH检测试剂盒
- 产品规格:100 Tests
| Fluoro NAD™ NEW! Fluorescent NAD/NADH Detection Kit – Detection of NAD/NADH in tissue extracts /cell samples |
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| Introduction | |||||||||
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The role of pyridine nucleotides (NAD/NADH) metabolism in health, is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation and are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling. This suggests that either the enzymes or NAD could be an important therapeutical target 1. |
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| Assay Principle | |||||||||
| The Fluoro NAD/NADH detection kit utilizes a non-fluorescent detection reagent, which is oxidized in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH.
Reaction: 1. NADH + non-fluorescent detection reagent+ electron coupler —–> fluorescent 2. NAD + enzyme coupled reaction ——-> NADH (then proceeds to reaction 1). excitation: 530-570nm and emission at 590-600nm
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| References | |||||||||
| Anthony A. Sauve NAD+ and Vitamin B3: From metabolism to therapies J. Pharmacol. Exp. Ther. 2007 : jpet.107.120758v1 |
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| Kit contents (for 100 assays) | |||||||||
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