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FITC标记山羊抗小鼠IgG(H+L)(A0568)

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FITC标记山羊抗小鼠IgG(H+L)
FITC标记山羊抗小鼠IgG(H+L)(A0568) 一键复制产品信息

产品编号: A0568

产品包装:100μl
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100μl

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352.00 340.00 10

价格: ¥ 352.00

促销价: ¥ 352.00

促销价: ¥ 352.00 340.00

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A0568 FITC标记山羊抗小鼠IgG(H+L) 100μl 352.00元

本FITC标记山羊抗小鼠IgG (H+L)(FITC-labeled Goat Anti-Mouse IgG (H+L))为进口分装，用于免疫荧光染色。
FITC是一种常用的绿色荧光探针。FITC的吸收(激发)和发射峰参见下表。

Fluorophore Absorption Peak (nm) Emission Peak (nm)
FITC 492 520

本抗体为用纯化的小鼠IgG免疫山羊，然后用亲和纯化柱对获得的抗血清进行纯化，并经过人IgG吸附纯化的优质二抗。对人IgG几乎没有结合能力。
本FITC标记山羊抗小鼠IgG (H+L)用于免疫荧光染色时的推荐稀释比例为1:500。实际实验操作过程中需根据抗原和抗体的具体情况适当调节荧光标记二抗的稀释比例，推荐的调节范围为1:200-1000。
本抗体如果用于常规的免疫染色，以每次检测需1毫升1:500稀释的荧光标记二抗计，至少可以检测50次。如果适当重复使用已经使用过的荧光标记二抗，至少可以多检测150-250次。
包装清单：

产品编号 产品名称 包装
A0568 FITC标记山羊抗小鼠IgG (H+L) 100μl
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保存条件：
-20℃避光保存，一年有效。
注意事项：
 本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 免疫荧光染色请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
2. 如果希望重复使用稀释的荧光标记二抗，稀释的荧光标记二抗4℃保存。

FITC标记山羊抗小鼠IgG(H+L)(A0568)
FITC标记山羊抗小鼠IgG(H+L)(A0568)

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FITC标记山羊抗小鼠IgG(H+L)(A0568)

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78. Pengcheng Wang, Juan Bai, Xuewei Liu, Mi Wang, Xianwei Wang, Ping Jiang
Tomatidine inhibits porcine epidemic diarrhea virus replication by targeting 3CL protease
Vet Res. 2020 Nov 11;51(1):136.;doi: 10.1186/s13567-020-00865-y. (IF 3.357)

79. Han Xian-Kui, Wang Hui-Fang, Shen Jing-Ran, Hou Yu-Rong, Chen Bo-Yu, Song Xiu-Jun
P38 Inhibition Prevents Herpes Simplex Virus Type 1 (HSV-1) Infection in Cultured Corneal Keratocytes
Curr Eye Res. 2020 Nov;45(11):1342-1351.;doi: 10.1080/02713683.2020.1748658. (IF 1.754)

80. Nansheng Wan, Xin Tang, Hui Ding, Yuxia Yan, Yan Zhuang, Chao Qi, Qianqian Chen, Wei Xie, Jing Zhang, Yan Wang, Maoli Liang, Wen Ning, Jie Cao
Influence of coexistence of mild OSA on airway mucus hypersecretion in patients with COPD
J Breath Res. 2020 Dec 18.;doi: 10.1088/1752-7163/abd52e. (IF 2.929)

81. Changxin Chen, Zhixin Hunag, Mucheng Wang, Zicheng Huang, Xiangbo Chen, Anye Huang, Binbin Zheng, Lishan Wu, Yi Liu, Xinwen Wang, Weifeng Xu
Endothelial transdifferentiation of human HGC-27 gastric cancer cells in vitro
Oncol Lett. 2020 Dec;20(6):303.;doi: 10.3892/ol.2020.12166. (IF 2.311)

82. Fangyuan Xing, Yongrong Liu, Ruifang Dong, Ye Cheng
miR-374 improves cerebral ischemia reperfusion injury by targeting Wnt5a
EXP ANIM TOKYO. 2021 Feb 6;70(1):126-136.;doi: 10.1538/expanim.20-0034. (IF 1.574)

83. Xiaoye Cheng, Zain-Ui Aabdin, Yan Wang, Nana Ma, Hongyu Dai, Xiaoli Shi, Xiangzhen Shen
Glutamine pretreatment protects bovine mammary epithelial cells from inflammation and oxidative stress induced by γ-d-glutamyl-meso-diaminopimelic acid (iE-DAP)
J Dairy Sci. 2021 Feb;104(2):2123-2139.;doi: 10.3168/jds.2020-18402. (IF 3.333)

84. Jiachao Xiong, Hong Qiang, Ting Li, Jiayi Zhao, Ziyu Wang, Fei Li, Jianwen Xu
Human adipose-derived stem cells promote seawater-immersed wound healing via proangiogenic effects
Aging (Albany NY). 2021 Mar 26;13(13):17118-17136.;doi: 10.18632/aging.202773.

85. Dai-Chao Ma, Nan-Nan Zhang, Yi-Na Zhang, Hui-Sheng Chen
Salvianolic Acids for Injection alleviates cerebral ischemia/reperfusion injury by switching M1/M2 phenotypes and inhibiting NLRP3 inflammasome/pyroptosis axis in microglia in vivo and in vitro
J Ethnopharmacol. 2021 Apr 24;270:113776.;doi: 10.1016/j.jep.2021.113776. (IF 3.69)

86. Yingqi Zhu, Xiangxue Gu, Miao Zhang, Xuan Lv, Chong Zhang, Jiaming Li, Zengjin Hu, Qiong Wu, Ruichen Zhang, Juanwen Wei, Rong Huang, Bei Wang, Qing Wang, Guijun Wang
Epigallocatechin-3-gallate exhibits antiviral effects against the duck Tembusu virus via blocking virus entry and upregulating type I interferons
Poult Sci. 2021 Apr;100(4):100989.;doi: 10.1016/j.psj.2021.01.012.

87. Rundong Jiang, Jiaqi Xu, Yuezhong Zhang, Xuanmeng Zhu, Jiachen Liu, Yurong Tan
Ligustrazine Alleviate Acute Lung Injury Through Suppressing Pyroptosis and Apoptosis of Alveolar Macrophages
Front Pharmacol. 2021 May 28;12:680512.;doi: 10.3389/fphar.2021.680512. (IF 4.225)

88. Cheng Wang, Shaobo Wang, Daixi Li, Peiqin Chen, Songling Han, Gaomei Zhao, Yin Chen, Jianqi Zhao, Jiachuan Xiong, Jingfei Qiu, Dong-Qing Wei, Jinghong Zhao, Junping Wang
Human Cathelicidin Inhibits SARS-CoV-2 Infection: Killing Two Birds with One Stone
ACS Infect Dis. 2021 Jun 11;7(6):1545-1554.;doi: 10.1021/acsinfecdis.1c00096. (IF 4.614)

89. Guobin Xie, Yuqi Zhou, Mingxuan Du, Qiqiang Wang, Jiajin Yi, Xiao-Kun Zhang
Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines
STAR Protoc. 2021 Jun 17;2(3):100495.;doi: 10.1016/j.xpro.2021.100495.

90. Qian Chai, Ziang Meng, Dexue Lu, Ziying Zhang, Meili Liu, Weihua Wu
Intermittent high glucose induces pyroptosis of rat H9C2 cardiomyocytes via sodium-glucose cotransporter 1
Mol Cell Biochem. 2021 Jun;476(6):2479-2489.;doi: 10.1007/s11010-021-04104-6. (IF 2.795)

91. Zhao Huang, Lang Gong, Zezhong Zheng, Qi Gao, Xiongnan Chen, Yang Chen, Xiaojun Chen, Runda Xu, Jiachen Zheng, Zhiying Xu, Shengxun Zhang, Heng Wang, Guihong Zhang
GS-441524 inhibits African swine fever virus infection in vitro
Antiviral Res. 2021 Jul;191:105081.;doi: 10.1016/j.antiviral.2021.105081.

92. Yang Bai, Yanli Yang, Yafen Gao, Duomao Lin, Zhaoqi Wang, Jun Ma
Melatonin postconditioning ameliorates anoxia/reoxygenation injury by regulating mitophagy and mitochondrial dynamics in a SIRT3-dependent manner
Eur J Pharmacol. 2021 Aug 5;904:174157.;doi: 10.1016/j.ejphar.2021.174157. (IF 3.263)

93. Zhaohui Liu, Zheng Hu, Xingdong Cai, Shengming Liu
SLC39A5 promotes lung adenocarcinoma cell proliferation by activating PI3K/AKT signaling
Pathol Res Pract. 2021 Aug;224:153541.;doi: 10.1016/j.prp.2021.153541. (IF 2.05)

94. Jiangyuan Guo, Bin Li, Jueqiong Wang, Ruoyi Guo, Ye Tian, Shuang Song, Li Guo
Protective effect and mechanism of nicotinamide adenine dinucleotide against optic neuritis in mice with experimental autoimmune encephalomyelitis
Int Immunopharmacol. 2021 Sep;98:107846.;doi: 10.1016/j.intimp.2021.107846. (IF 3.943)

95. Lili Tan, Jie Lu, Limin Liu, Lu Li
Fatty acid binding protein 3 deficiency limits atherosclerosis development via macrophage foam cell formation inhibition
Exp Cell Res. 2021 Oct 1;407(1):112768.;doi: 10.1016/j.yexcr.2021.112768. (IF 3.383)

96. Weiwei Liu, Liqiang Shi, Qiang Wan, Yansheng Wu, Di Huang, Jiaoying Ou, Qiuyu Liu, Xin Guan, Yuzhu Yang, Xiaotian Zhang, Jiandong Gao
Huangqi Guizhi Wuwu Decoction attenuates Podocyte cytoskeletal protein damage in IgA nephropathy rats by regulating AT1R/Nephrin/c-Abl pathway
Biomed Pharmacother. 2021 Oct;142:111907.;doi: 10.1016/j.biopha.2021.111907. (IF 4.545)

97. Lei Wang, Bojuan Lang, Youdong Zhou, Jinyang Ma, Keqi Hu
Up-regulation of miR-663a inhibits the cancer stem cell-like properties of glioma via repressing the KDM2A-mediated TGF-β/SMAD signaling pathway
Cell Cycle. 2021 Oct;20(19):1935-1952.;doi: 10.1080/15384101.2021.1966962. (IF 3.699)

98. Jing-Hua Song, Hong-Yan Jia, Tian-Peng Shao, Zhi-Bao Liu, Yuan-Ping Zhao
Hydrogen gas post-conditioning alleviates cognitive dysfunction and anxiety-like behavior in a rat model of subarachnoid hemorrhage
Exp Ther Med. 2021 Oct;22(4):1121.;doi: 10.3892/etm.2021.10555. (IF 1.785)

99. Tingjiao Liu, Qianqian Li, Shanshan Yang, Tingting Zhao, Jinghan Lin, Ting Ju, Zhaohui Wen
CNTs-CaP/chitosan-coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway
Cell Biochem Funct. 2021 Oct;39(7):908-920.;doi: 10.1002/cbf.3662. (IF 2.632)

100. Xin Wang, Yu Hu, Yaguang Wang, Dapeng Shen, Guizhou Tao
CLEC5A knockdown protects against cardiac dysfunction after myocardial infarction by suppressing macrophage polarization, NLRP3 inflammasome activation, and pyroptosis
Biochem Cell Biol. 2021 Oct;99(5):655-665.;doi: 10.1139/bcb-2020-0672. (IF 2.46)

101. Shuang Zhang, Sihan Lin, Qiaofei Tang, Zhiyong Yan
Knockdown of miR‑205‑5p alleviates the inflammatory response in allergic rhinitis by targeting B‑cell lymphoma 6
Mol Med Rep. 2021 Nov;24(5):818.;doi: 10.3892/mmr.2021.12458. (IF 2.1)

102. Xi Su, Wenqiang Li, Zhen Li, Kang Liu, Meng Song, Minglong Shao, Luxian Lv, Xulu Chang
Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats
Ecotoxicol Environ Saf. 2021 Dec 1;225:112806.;doi: 10.1016/j.ecoenv.2021.112806.

103. Menghuai Sun, Qian Lin, Chunyang Wang, Jiao Xing, Kunlong Yan, Zhifeng Liu, Yu Jin, Carol J Cardona, Zheng Xing
Enterovirus A71 2B Inhibits Interferon-Activated JAK/STAT Signaling by Inducing Caspase-3-Dependent Karyopherin-α1 Degradation
Front Microbiol. 2021 Dec 21;12:762869.;doi: 10.3389/fmicb.2021.762869. (IF 4.235)

104. Wen-Chong Sun, Ling Pei
Dexmedetomidine attenuates propofol-induced apoptosis of neonatal hippocampal astrocytes by inhibiting the Bcl2l1 signalling pathway
Eur J Neurosci. 2021 Dec;54(11):7775-7789.;doi: 10.1111/ejn.15517. (IF 3.115)

105. Hui Zheng, Zhihuang Qiu, Tianci Chai, Jian He, Yuling Zhang, Chaoyun Wang, Jianqiang Ye, Xiaohui Wu, Yumei Li, Li Zhang, Liangwan Chen
Insulin Resistance Promotes the Formation of Aortic Dissection by Inducing the Phenotypic Switch of Vascular Smooth Muscle Cells
Front Cardiovasc Med. 2022 Feb 3;8:732122.;doi: 10.3389/fcvm.2021.732122.

重组FITC标记人HLA-A*11:01&B2M&KRAS WT(VVVGAGGVGK)单体蛋白 Recombinant FITC-Labeled Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein,His-Avi Tag

发表于由

重组FITC标记人HLA-A*11:01&B2M&KRAS WT(VVVGAGGVGK)单体蛋白 Recombinant FITC-Labeled Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名（Synonyms）

MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD

表达区间及表达系统（Source）

FITC-Labeled Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGAGGVGK peptide.[Accession | AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGAGGVGK]

分子量大小（Molecular Weight）

The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE

制剂（Formulation）

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人HLA-A*11:01&B2M&KRAS WT(VVVGAGGVGK)单体蛋白 Recombinant FITC-Labeled Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein,His-Avi Tag

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重组FITC标记人HLA-A*11:01&B2M&KRAS WT(VVVGAGGVGK)单体蛋白 Recombinant FITC-Labeled Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein,His-Avi Tag

暂无内容

 

性能参数

分子别名（Synonyms）

MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD

表达区间及表达系统（Source）

FITC-Labeled Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGAGGVGK peptide.[Accession | AAV53343.1(HLA-A*11:01)&P61769(B2M)&VVVGAGGVGK]

分子量大小（Molecular Weight）

The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE

制剂（Formulation）

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人HLA-A*11:01&B2M&KRAS WT(VVVGAGGVGK)单体蛋白 Recombinant FITC-Labeled Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein,His-Avi Tag

暂无内容

重组FITC标记人HLA-A*11:01&B2M&KRAS WT(VVVGAGGVGK)单体蛋白 Recombinant FITC-Labeled Human HLA-A*11:01&B2M&KRAS WT (VVVGAGGVGK) Monomer Protein,His-Avi Tag

暂无内容

重组FITC 兼容人BCMA/TNFRSF17蛋白 Recombinant FITC-Compatible Human BCMA/TNFRSF17 Protein,His Tag

发表于由

重组FITC 兼容人BCMA/TNFRSF17蛋白 Recombinant FITC-Compatible Human BCMA/TNFRSF17 Protein,His Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名（Synonyms）

BCMA;TNFRSF13A;TNFRSF17;CD269

表达区间及表达系统（Source）

FITC-Compatible Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Met1-Ala54.[Accession | Q02223-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 37-48 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

制剂（Formulation）

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC 兼容人BCMA/TNFRSF17蛋白 Recombinant FITC-Compatible Human BCMA/TNFRSF17 Protein,His Tag

暂无内容

重组FITC 兼容人BCMA/TNFRSF17蛋白 Recombinant FITC-Compatible Human BCMA/TNFRSF17 Protein,His Tag

暂无内容

 

性能参数

分子别名（Synonyms）

BCMA;TNFRSF13A;TNFRSF17;CD269

表达区间及表达系统（Source）

FITC-Compatible Human BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Met1-Ala54.[Accession | Q02223-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 37-48 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

制剂（Formulation）

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC 兼容人BCMA/TNFRSF17蛋白 Recombinant FITC-Compatible Human BCMA/TNFRSF17 Protein,His Tag

暂无内容

重组FITC 兼容人BCMA/TNFRSF17蛋白 Recombinant FITC-Compatible Human BCMA/TNFRSF17 Protein,His Tag

暂无内容

重组FITC标记的人MSLN/间皮蛋白(hFc标签) FITC-Labeled Human MSLN/Mesothelin Proteing

发表于由

重组FITC标记的人MSLN/间皮蛋白(hFc标签) FITC-Labeled Human MSLN/Mesothelin Proteing

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名（Synonyms）

Mesothelin;MSLN; MPF; SMR

表达区间及表达系统（Source）

FITC-Labeled Human MSLN/Mesothelin Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu296-Gly580.[Accession | Q13421-2]

分子量大小（Molecular Weight）

The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE

制剂（Formulation）

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名（Synonyms）

Mesothelin;MSLN; MPF; SMR

表达区间及表达系统（Source）

FITC-Labeled Human MSLN/Mesothelin Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Glu296-Gly580.[Accession | Q13421-2]

分子量大小（Molecular Weight）

The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE

制剂（Formulation）

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人VEGF165蛋白 Recombinant FITC-Labeled Human VEGF165 Protein,His-Avi Tag

发表于由

重组FITC标记人VEGF165蛋白 Recombinant FITC-Labeled Human VEGF165 Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名（Synonyms）

VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165

表达区间及表达系统（Source）

FITC-Labeled Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala27-Arg191.[Accession | P15692-4]

分子量大小（Molecular Weight）

The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-33 kDa under reduced (R) condition, 45-55 kDa under Non reducing (N) condition based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized FITC-Labeled Human VEGF165, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 22.3ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名（Synonyms）

VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165

表达区间及表达系统（Source）

FITC-Labeled Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala27-Arg191.[Accession | P15692-4]

分子量大小（Molecular Weight）

The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-33 kDa under reduced (R) condition, 45-55 kDa under Non reducing (N) condition based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized FITC-Labeled Human VEGF165, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-VEGF165 Antibody, hFc Tag with the EC50 of 22.3ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人NKG2D/CD314蛋白 Recombinant FITC-Labeled Human NKG2D/CD314 Protein,hFc-Flag Tag

发表于由

重组FITC标记人NKG2D/CD314蛋白 Recombinant FITC-Labeled Human NKG2D/CD314 Protein,hFc-Flag Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名（Synonyms）

CD314; D12S2489E; KLR; NKG2-D; NKG2D

表达区间及表达系统（Source）

FITC-Labeled Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus. It contains Phe78-Val216.[Accession | P26718]

分子量大小（Molecular Weight）

The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

制剂（Formulation）

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人NKG2D/CD314蛋白 Recombinant FITC-Labeled Human NKG2D/CD314 Protein,hFc-Flag Tag

暂无内容

重组FITC标记人NKG2D/CD314蛋白 Recombinant FITC-Labeled Human NKG2D/CD314 Protein,hFc-Flag Tag

暂无内容

 

性能参数

分子别名（Synonyms）

CD314; D12S2489E; KLR; NKG2-D; NKG2D

表达区间及表达系统（Source）

FITC-Labeled Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus. It contains Phe78-Val216.[Accession | P26718]

分子量大小（Molecular Weight）

The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

制剂（Formulation）

Supplied as 0.22μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人NKG2D/CD314蛋白 Recombinant FITC-Labeled Human NKG2D/CD314 Protein,hFc-Flag Tag

暂无内容

重组FITC标记人NKG2D/CD314蛋白 Recombinant FITC-Labeled Human NKG2D/CD314 Protein,hFc-Flag Tag

暂无内容

FITC标记山羊抗人IgG Goat Anti-Human IgG(H+L)

发表于由

FITC标记山羊抗人IgG Goat Anti-Human IgG(H+L)

产品说明书

FAQ

COA

已发表文献

产品信息

产品名称

产品编号

规格

FITC-AffiniPure Goat Anti-Human IgG(H+L)

34856JP60

100 μL

 

产品简介

本品是由FITC标记的山羊抗人IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的人IgG分子，也会与其他人免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。该荧光基团光谱性质同Alexa Fluor 488，Amax（最大激发波长）为492nm，Emax（最大发射波长）为520nm。

本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）以及免疫组化（IHC）等。要做多标（multiple-labeling）实验，建议使用与相近物种的血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。。

 

产品信息

抗体浓度（Antibody Concentration）

100 µL (0.75 mg/ml)

缓冲液（Buffer）

0.005M 磷酸钠，0.125M 氯化钠，pH7.6

稳定剂（Stabilizer）

7.5mg/ml BSA（无IgG，无蛋白酶）

防腐剂（Preservative）

0.025%叠氮化钠

 

产品应用

建议稀释浓度：1:25-1:100(for most application)

 

储存条件

-25 ~ -15℃分装保存，尽量避免反复冻融。

 

注意事项

1）为了您的安全和健康，请穿实验服并戴一次性手套操作；

2）本产品仅作科研用途！

Ver.CN20240228

 

 

FITC标记山羊抗人IgG Goat Anti-Human IgG(H+L)

暂无内容

FITC标记山羊抗人IgG Goat Anti-Human IgG(H+L)

暂无内容

产品信息

产品名称

产品编号

规格

FITC-AffiniPure Goat Anti-Human IgG(H+L)

34856JP60

100 μL

 

产品简介

本品是由FITC标记的山羊抗人IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的人IgG分子，也会与其他人免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。该荧光基团光谱性质同Alexa Fluor 488，Amax（最大激发波长）为492nm，Emax（最大发射波长）为520nm。

本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）以及免疫组化（IHC）等。要做多标（multiple-labeling）实验，建议使用与相近物种的血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。。

 

产品信息

抗体浓度（Antibody Concentration）

100 µL (0.75 mg/ml)

缓冲液（Buffer）

0.005M 磷酸钠，0.125M 氯化钠，pH7.6

稳定剂（Stabilizer）

7.5mg/ml BSA（无IgG，无蛋白酶）

防腐剂（Preservative）

0.025%叠氮化钠

 

产品应用

建议稀释浓度：1:25-1:100(for most application)

 

储存条件

-25 ~ -15℃分装保存，尽量避免反复冻融。

 

注意事项

1）为了您的安全和健康，请穿实验服并戴一次性手套操作；

2）本产品仅作科研用途！

Ver.CN20240228

 

 

FITC标记山羊抗人IgG Goat Anti-Human IgG(H+L)

暂无内容

FITC标记山羊抗人IgG Goat Anti-Human IgG(H+L)

暂无内容

FITC标记山羊抗兔IgG抗体 FITC山羊抗兔IgG抗体|FITC Goat Anti-Rabbit IgG

发表于由

FITC标记山羊抗兔IgG抗体 FITC山羊抗兔IgG抗体|FITC Goat Anti-Rabbit IgG

产品说明书

FAQ

COA

已发表文献

产品描述

 

本品是由FITC标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。该荧光基团光谱性质同Alexa Fluor 488，Amax（最大激发波长）为492nm，Emax（最大发射波长）为520nm。

本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）以及免疫组化（IHC）等。要做多标（multiple-labeling）实验，建议使用与相近物种的血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

产品应用

建议稀释浓度：1:25-1:100（For most application）

产品性质 

抗体浓度（Antibody Concentration）

100µl (0.75mg/ml)

缓冲液（Buffer）

0.005M 磷酸钠，0.125M 氯化钠, pH 7.6

稳定剂（Stabilizer）

7.5mg/ml BSA（无IgG，蛋白酶），50% 甘油

荧光素（Fluorophore）

FITC-isomer 1, Amax =492nm, Emax=520nm

防腐剂（Preservative）

0.025% 叠氮化钠

原料来源（Source of Material）

Jackson Immunoresearch 111-095-003

运输与保存方法

冰袋运输。-20℃分装保存，尽量避免反复冻融。有效期1年。

注意事项

1）本品含叠氮化钠，对人体有害，请注意适当防护。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

3）本产品仅作科研用途！

 

FITC标记山羊抗兔IgG抗体 FITC山羊抗兔IgG抗体|FITC Goat Anti-Rabbit IgG

暂无内容

[1] Wang Z, Gong X, Li J, et al. Oxygen-Delivering Polyfluorocarbon Nanovehicles Improve Tumor Oxygenation and Potentiate Photodynamic-Mediated Antitumor Immunity. ACS Nano. 2021;15(3):5405-5419. doi:10.1021/acsnano.1c00033(IF:15.881)
[2] Bao L, Dou G, Tian R, et al. Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution. Bioact Mater. 2021;9:183-197. Published 2021 Aug 27. doi:10.1016/j.bioactmat.2021.08.008(IF:14.593)
[3] Shi X, Cheng Y, Wang J, et al. 3D printed intelligent scaffold prevents recurrence and distal metastasis of breast cancer. Theranostics. 2020;10(23):10652-10664. Published 2020 Aug 29. doi:10.7150/thno.47933(IF:11.556)
[4] Li Z, Wu M, Liu S, et al. Apoptotic vesicles activate autophagy in recipient cells to induce angiogenesis and dental pulp regeneration [published online ahead of print, 2022 May 10]. Mol Ther. 2022;S1525-0016(22)00304-5. doi:10.1016/j.ymthe.2022.05.006(IF:11.454)
[5] Chen H, Wang X, Wang J, et al. In vitroadipogenesis and long-term adipocyte culture in adipose tissue-derived cell banks. Biofabrication. 2021;13(3):10.1088/1758-5090/ac0610. Published 2021 Jul 5. doi:10.1088/1758-5090/ac0610(IF:10.020)
[6] Wang Y, Lin YX, Qiao SL, et al. Polymeric nanoparticles promote macrophage reversal from M2 to M1 phenotypes in the tumor microenvironment. Biomaterials. 2017;112:153-163. doi:10.1016/j.biomaterials.2016.09.034(IF:8.387)
[7] Yuan J, Jiang X, Lan H, et al. Multi-Omics Analysis of the Therapeutic Value of MAL2 Based on Data Mining in Human Cancers. Front Cell Dev Biol. 2022;9:736649. Published 2022 Jan 17. doi:10.3389/fcell.2021.736649(IF:6.684)
[8] Tao Y, Qiao SM, Lv CJ, et al. Phytoestrogen arctigenin preserves the mucus barrier in inflammatory bowel diseases by inhibiting goblet cell apoptosis via the ERβ/TRIM21/PHB1 pathway [published online ahead of print, 2022 May 22]. Phytother Res. 2022;10.1002/ptr.7495. doi:10.1002/ptr.7495(IF:5.882)
[9] Li Z, You L, Yan D, James AA, Huang Y, Tan A. Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination. PLoS Genet. 2018;14(2):e1007245. Published 2018 Feb 23. doi:10.1371/journal.pgen.1007245(IF:5.540)
[10] Tao Y, Yue M, Lv C, et al. Pharmacological activation of ERβ by arctigenin maintains the integrity of intestinal epithelial barrier in inflammatory bowel diseases. FASEB J. 2020;34(2):3069-3090. doi:10.1096/fj.201901638RR(IF:5.391)
[11] Li Z, Liu S, Fu T, Peng Y, Zhang J. Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):351. Published 2019 Nov 27. doi:10.1186/s13287-019-1441-4(IF:4.627)
[12] Li Z, You L, Zhang Q, Yu Y, Tan A. A Targeted In-Fusion Expression System for Recombinant Protein Production in Bombyx mori. Front Genet. 2022;12:816075. Published 2022 Jan 4. doi:10.3389/fgene.2021.816075(IF:4.599)
[13] Zheng C, Wu SM, Lian H, et al. Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways. J Cell Mol Med. 2019;23(3):1963-1975. doi:10.1111/jcmm.14098(IF:4.302)
[14] Wang H, Zhang Z, Guan J, Lu W, Zhan C. Unraveling GLUT-mediated transcytosis pathway of glycosylated nanodisks. Asian J Pharm Sci. 2021;16(1):120-128. doi:10.1016/j.ajps.2020.07.001(IF:3.968)
[15] Chen L, Lv L, Zhang L, et al. Metformin ameliorates bladder dysfunction in a rat model of partial bladder outlet obstruction. Am J Physiol Renal Physiol. 2021;320(5):F838-F858. doi:10.1152/ajprenal.00625.2020(IF:3.377)
[16] Yang LY, Liu XF, Yang Y, et al. Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities. Acta Pharmacol Sin. 2017;38(1):56-68. doi:10.1038/aps.2016.89(IF:3.166)
[17] Wang Y, Jiang C, Cong S, Guo C, Yan Z. Extracellular matrix deposited by Wharton's jelly mesenchymal stem cells enhances cell expansion and tissue specific lineage potential. Am J Transl Res. 2018;10(11):3465-3480. Published 2018 Nov 15. (IF:3.061)
[18] Xue Z, Zhuang J, Bai H, et al. VDR mediated HSD3B1 to regulate lipid metabolism and promoted testosterone synthesis in mouse Leydig cells. Genes Genomics. 2022;44(5):583-592. doi:10.1007/s13258-022-01232-1(IF:1.839)

产品描述

 

本品是由FITC标记的山羊抗兔IgG（H+L），使用抗原偶联的琼脂糖微珠从山羊抗血清内亲和色谱纯化所得。免疫电泳和/或ELISA法检测显示本品特异性结合完整的兔IgG分子，也会与其他兔免疫球蛋白的轻链结合。可能会与其他物种免疫球蛋白发生交叉反应，但不会识别非免疫球蛋白类的血清蛋白。该荧光基团光谱性质同Alexa Fluor 488，Amax（最大激发波长）为492nm，Emax（最大发射波长）为520nm。

本品适合做单标实验，广泛应用于多种免疫学实验如免疫细胞化学（ICC），流式细胞术（FC）以及免疫组化（IHC）等。要做多标（multiple-labeling）实验，建议使用与相近物种的血清蛋白或者免疫球蛋白预先经过亲和吸附处理的二抗。

产品应用

建议稀释浓度：1:25-1:100（For most application）

产品性质 

抗体浓度（Antibody Concentration）

100µl (0.75mg/ml)

缓冲液（Buffer）

0.005M 磷酸钠，0.125M 氯化钠, pH 7.6

稳定剂（Stabilizer）

7.5mg/ml BSA（无IgG，蛋白酶），50% 甘油

荧光素（Fluorophore）

FITC-isomer 1, Amax =492nm, Emax=520nm

防腐剂（Preservative）

0.025% 叠氮化钠

原料来源（Source of Material）

Jackson Immunoresearch 111-095-003

运输与保存方法

冰袋运输。-20℃分装保存，尽量避免反复冻融。有效期1年。

注意事项

1）本品含叠氮化钠，对人体有害，请注意适当防护。

2）为了您的安全和健康，请穿实验服并戴一次性手套操作。

3）本产品仅作科研用途！

 

FITC标记山羊抗兔IgG抗体 FITC山羊抗兔IgG抗体|FITC Goat Anti-Rabbit IgG

暂无内容

[1] Wang Z, Gong X, Li J, et al. Oxygen-Delivering Polyfluorocarbon Nanovehicles Improve Tumor Oxygenation and Potentiate Photodynamic-Mediated Antitumor Immunity. ACS Nano. 2021;15(3):5405-5419. doi:10.1021/acsnano.1c00033(IF:15.881)
[2] Bao L, Dou G, Tian R, et al. Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution. Bioact Mater. 2021;9:183-197. Published 2021 Aug 27. doi:10.1016/j.bioactmat.2021.08.008(IF:14.593)
[3] Shi X, Cheng Y, Wang J, et al. 3D printed intelligent scaffold prevents recurrence and distal metastasis of breast cancer. Theranostics. 2020;10(23):10652-10664. Published 2020 Aug 29. doi:10.7150/thno.47933(IF:11.556)
[4] Li Z, Wu M, Liu S, et al. Apoptotic vesicles activate autophagy in recipient cells to induce angiogenesis and dental pulp regeneration [published online ahead of print, 2022 May 10]. Mol Ther. 2022;S1525-0016(22)00304-5. doi:10.1016/j.ymthe.2022.05.006(IF:11.454)
[5] Chen H, Wang X, Wang J, et al. In vitroadipogenesis and long-term adipocyte culture in adipose tissue-derived cell banks. Biofabrication. 2021;13(3):10.1088/1758-5090/ac0610. Published 2021 Jul 5. doi:10.1088/1758-5090/ac0610(IF:10.020)
[6] Wang Y, Lin YX, Qiao SL, et al. Polymeric nanoparticles promote macrophage reversal from M2 to M1 phenotypes in the tumor microenvironment. Biomaterials. 2017;112:153-163. doi:10.1016/j.biomaterials.2016.09.034(IF:8.387)
[7] Yuan J, Jiang X, Lan H, et al. Multi-Omics Analysis of the Therapeutic Value of MAL2 Based on Data Mining in Human Cancers. Front Cell Dev Biol. 2022;9:736649. Published 2022 Jan 17. doi:10.3389/fcell.2021.736649(IF:6.684)
[8] Tao Y, Qiao SM, Lv CJ, et al. Phytoestrogen arctigenin preserves the mucus barrier in inflammatory bowel diseases by inhibiting goblet cell apoptosis via the ERβ/TRIM21/PHB1 pathway [published online ahead of print, 2022 May 22]. Phytother Res. 2022;10.1002/ptr.7495. doi:10.1002/ptr.7495(IF:5.882)
[9] Li Z, You L, Yan D, James AA, Huang Y, Tan A. Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination. PLoS Genet. 2018;14(2):e1007245. Published 2018 Feb 23. doi:10.1371/journal.pgen.1007245(IF:5.540)
[10] Tao Y, Yue M, Lv C, et al. Pharmacological activation of ERβ by arctigenin maintains the integrity of intestinal epithelial barrier in inflammatory bowel diseases. FASEB J. 2020;34(2):3069-3090. doi:10.1096/fj.201901638RR(IF:5.391)
[11] Li Z, Liu S, Fu T, Peng Y, Zhang J. Microtubule destabilization caused by silicate via HDAC6 activation contributes to autophagic dysfunction in bone mesenchymal stem cells. Stem Cell Res Ther. 2019;10(1):351. Published 2019 Nov 27. doi:10.1186/s13287-019-1441-4(IF:4.627)
[12] Li Z, You L, Zhang Q, Yu Y, Tan A. A Targeted In-Fusion Expression System for Recombinant Protein Production in Bombyx mori. Front Genet. 2022;12:816075. Published 2022 Jan 4. doi:10.3389/fgene.2021.816075(IF:4.599)
[13] Zheng C, Wu SM, Lian H, et al. Low-intensity pulsed ultrasound attenuates cardiac inflammation of CVB3-induced viral myocarditis via regulation of caveolin-1 and MAPK pathways. J Cell Mol Med. 2019;23(3):1963-1975. doi:10.1111/jcmm.14098(IF:4.302)
[14] Wang H, Zhang Z, Guan J, Lu W, Zhan C. Unraveling GLUT-mediated transcytosis pathway of glycosylated nanodisks. Asian J Pharm Sci. 2021;16(1):120-128. doi:10.1016/j.ajps.2020.07.001(IF:3.968)
[15] Chen L, Lv L, Zhang L, et al. Metformin ameliorates bladder dysfunction in a rat model of partial bladder outlet obstruction. Am J Physiol Renal Physiol. 2021;320(5):F838-F858. doi:10.1152/ajprenal.00625.2020(IF:3.377)
[16] Yang LY, Liu XF, Yang Y, et al. Biochemical features of the adhesion G protein-coupled receptor CD97 related to its auto-proteolysis and HeLa cell attachment activities. Acta Pharmacol Sin. 2017;38(1):56-68. doi:10.1038/aps.2016.89(IF:3.166)
[17] Wang Y, Jiang C, Cong S, Guo C, Yan Z. Extracellular matrix deposited by Wharton's jelly mesenchymal stem cells enhances cell expansion and tissue specific lineage potential. Am J Transl Res. 2018;10(11):3465-3480. Published 2018 Nov 15. (IF:3.061)
[18] Xue Z, Zhuang J, Bai H, et al. VDR mediated HSD3B1 to regulate lipid metabolism and promoted testosterone synthesis in mouse Leydig cells. Genes Genomics. 2022;44(5):583-592. doi:10.1007/s13258-022-01232-1(IF:1.839)

Annexin V-FITC/PI细胞凋亡检测试剂盒|Annexin V-FITC/PI Apoptosis Detection Kit

发表于由

Annexin V-FITC/PI细胞凋亡检测试剂盒|Annexin V-FITC/PI Apoptosis Detection Kit

产品说明书

FAQ

COA

已发表文献

产品描述

Annexin V-FITC/PI细胞凋亡检测试剂盒是用FITC标记的Annexin V作为探针，来检测细胞早期凋亡的发生。

其检测原理为：在正常的活细胞中，磷脂酰丝氨酸（phosphotidylserine，PS）位于细胞膜的内侧，但在早期凋亡的细胞中，PS 从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中。Annexin-Ⅴ（膜联蛋白-V）是一种分子量为35-36 kDa的Ca2+ 依赖性磷脂结合蛋白，能与PS高亲和力结合，可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。

另外，本试剂盒中还提供了碘化丙啶（Propidium Iodide，PI）用来区分存活的早期细胞和坏死或晚期凋亡细胞。PI是一种核酸染料，它不能透过正常细胞或早期凋亡细胞的完整的细胞膜，但可以透过凋亡晚期和坏死细胞的细胞膜而使细胞核染红。因此，将Annexin V与PI联合使用时，PI 则被排除在活细胞（Annexin V-/PI-）和早期凋亡细胞（Annexin V+/PI-）之外，而晚期凋亡细胞和坏死细胞同时被FITC 和PI 结合染色呈现双阳性（Annexin V+/PI+）。

本试剂盒可用于流式细胞仪、荧光显微镜进行检测。

 

产品组分

编号

组分

产品编号/规格

40302ES20（20T）

40302ES50（50T）

40302ES60（100T）

40302-A

Annexin V-FITC

100 μL

250 μL

500 μL

40302-B

PI Staining Solution

200 μL

500 μL

1.0 mL

40302-C

1×Binding Buffer

10 mL

25 mL

50 mL

 

运输与保存方法

冰袋（wet ice）运输。-20℃避光保存，避免反复冻融，一年有效。

【注】：如果需要在短时间内多次重复使用，可以在4℃避光保存，半年有效。

 

注意事项

1）由于细胞凋亡是一个快速的过程，建议样品在染色后1小时之内进行分析。

2） 对于贴壁细胞，消化是一个关键步骤。贴壁细胞诱导细胞凋亡时如有漂浮细胞，需收集漂浮细胞和贴壁细胞后合并染色。处理贴壁细胞时要小心操作，尽量避免人为的损伤。胰酶消化时间过短，细胞需要用力吹打才能脱落，容易造成细胞膜的损伤；PI摄入过多，消化时间过长，细胞膜同样易造成损伤，甚至会影响细胞膜上磷脂酰丝氨酸与Annexin V-FITC的结合。消化时将胰酶铺满孔板底后，轻摇使胰酶与细胞充分接触，然后倒掉大部分胰酶，利用剩余少量胰酶再消化一段时间，待细胞间空隙增大，瓶底呈花斑状即可终止。在消化液中尽量不用EDTA，EDTA会影响Annexin V与PS的结合。

3）如果样品来源于血液，请务必除去血液中的血小板。因为血小板含有PS，能与Annexin V结合，从而干扰实验结果。可以使用含有EDTA的缓冲剂并在200 g离心洗去血小板。

4）试剂在开盖前请短暂离心，将盖内壁上的液体甩至管底，避免开盖时液体洒落。

5）Annexin V-FITC和PI是光敏物质，在操作时请注意避光。

6）本产品仅作科研用途！

 

操作方法

1.1 样品染色

1）悬浮细胞：300 g，4℃离心5 min收集细胞。

贴壁细胞：用不含EDTA的胰酶消化后，300 g，4℃离心5 min收集细胞。胰酶消化时间不宜过长，以防引起假阳性。

2）用预冷的PBS洗涤细胞2次，每次均需300 g，4℃离心5 min。收集1～5×105细胞。

3）吸弃PBS，加入100 μL 1×Binding Buffer重悬细胞。

4）加入5 μL Annexin V-FITC和10 μLPI Staining Solution，轻轻混匀。

5）避光、室温反应10-15 min。

6）加入400 μL 1×Binding Buffer，混匀后放置于冰上，样品在1小时内用流式细胞仪或荧光显微镜检测。

【注】：为了避免洗涤细胞时损失细胞，在吸液时可以用大的Tip头套上小的Tip头吸液。

1.2 样品分析

A．流式细胞仪分析：

FITC最大激发波长为488 nm，最大发射波长525 nm，FITC的绿色荧光在FL1通道检测；PI-DNA复合物的最大激发波长为535 nm，最大发射波长为615 nm，PI的红色荧光在FL2或FL3通道检测。用CellQuest等软件进行分析，绘制双色散点图（two-color dot plot），FITC为横坐标，PI为纵坐标。典型的实验中，细胞可以分成三个亚群，活细胞仅有很低强度的背景荧光，早期凋亡细胞仅有较强的绿色荧光，晚期凋亡细胞有绿色和红色荧光双重染色。

B．荧光显微镜分析：

1）滴一滴用Annexin V-FITC/PI双染的细胞悬液于载玻片上，并用盖玻片盖上细胞。

【注】：对于贴壁细胞，可直接用盖玻片培养细胞并诱导细胞凋亡。

2）在荧光显微镜下用双色滤光片观察。Annexin V-FITC荧光信号呈绿色，PI荧光信号呈红色。

 

相关产品

产品名称

货号

规格

Cell Cycle and Apoptosis Analysis Kit

细胞周期与细胞凋亡检测试剂盒

40301ES50

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40301ES60

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Annexin V-EGFP/PI 细胞凋亡检测试剂盒

Annexin V-EGFP/PI Apoptosis Detection Kit

40303ES20

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Annexin V-Alexa Fluor 647/PI 细胞凋亡检测试剂盒

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit

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40304ES50

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Annexin V-Alexa Fluor 488/PI 细胞凋亡检测试剂盒

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit

40305ES20

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40305ES50

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Annexin V-PE/7-AAD细胞凋亡检测试剂盒

Annexin V-PE/7-AAD Apoptosis Detection Kit

40310ES20

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40310ES50

50 T

40310ES60

100 T

 

 

HB220609

Q：Annexin V 和 JC-1、Tunel 细胞凋亡检测的区别？

A: Annexin V 是检测细胞早期凋亡的试剂，JC-1 是检测细胞中期凋亡的试剂、Tunel 是检测细胞晚期凋亡的试剂。

Q：Annexin V 和JC-1、Tunel 细胞凋亡检测的可以应用到植物或是细菌（原核生物） 吗？

A：可以，但是需要制备原生质体，因为植物细胞或是细菌（原核生物）含有细胞壁，具体的染液使用剂量只需浸没细胞即可，染色时间对于不同细胞有一定的不同。

Q：40302ES Annexin V-FITC/PI 细胞凋亡检测试剂盒里的PI的浓度是多少呢？

A：20ug/ml。

Q:实验结果如何判断？

A：活细胞（Annexin V-/PI-）

  早期凋亡细胞（Annexin V+/PI-）

  晚期凋亡细胞和坏死细胞呈现双阳性（Annexin V+/PI+）

  裸核（Annexin V-/PI+）

Q: ​Annexin V和TUNEL有什么区别？

A：末端脱氧核苷酸转移酶 dUTP 缺口末端标记 (TUNEL) 是一种染色方法，用于识别细胞内 DNA 片段化位点——晚期细胞凋亡的标志性特征。 它使用酶末端脱氧核苷酸转移酶 (TdT) 将修饰的 dNTP（例如 dUTP）连接到片段化 DNA 链的 3'-羟基末端。 dNTPs 通常用荧光团修饰以促进量化和/或可视化。

Annexin V 染色通过结合由于细胞膜不对称性丧失而暴露在细胞外的 PS 残基来识别细胞凋亡的早期阶段。 Annexin V 通常用 FITC 等荧光团标记，以促进凋亡细胞的检测。

Annexin V-FITC/PI细胞凋亡检测试剂盒|Annexin V-FITC/PI Apoptosis Detection Kit

 

 

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产品描述

Annexin V-FITC/PI细胞凋亡检测试剂盒是用FITC标记的Annexin V作为探针，来检测细胞早期凋亡的发生。

其检测原理为：在正常的活细胞中，磷脂酰丝氨酸（phosphotidylserine，PS）位于细胞膜的内侧，但在早期凋亡的细胞中，PS 从细胞膜的内侧翻转到细胞膜的表面，暴露在细胞外环境中。Annexin-Ⅴ（膜联蛋白-V）是一种分子量为35-36 kDa的Ca2+ 依赖性磷脂结合蛋白，能与PS高亲和力结合，可通过细胞外侧暴露的磷脂酰丝氨酸与凋亡早期细胞的胞膜结合。

另外，本试剂盒中还提供了碘化丙啶（Propidium Iodide，PI）用来区分存活的早期细胞和坏死或晚期凋亡细胞。PI是一种核酸染料，它不能透过正常细胞或早期凋亡细胞的完整的细胞膜，但可以透过凋亡晚期和坏死细胞的细胞膜而使细胞核染红。因此，将Annexin V与PI联合使用时，PI 则被排除在活细胞（Annexin V-/PI-）和早期凋亡细胞（Annexin V+/PI-）之外，而晚期凋亡细胞和坏死细胞同时被FITC 和PI 结合染色呈现双阳性（Annexin V+/PI+）。

本试剂盒可用于流式细胞仪、荧光显微镜进行检测。

 

产品组分

编号

组分

产品编号/规格

40302ES20（20T）

40302ES50（50T）

40302ES60（100T）

40302-A

Annexin V-FITC

100 μL

250 μL

500 μL

40302-B

PI Staining Solution

200 μL

500 μL

1.0 mL

40302-C

1×Binding Buffer

10 mL

25 mL

50 mL

 

运输与保存方法

冰袋（wet ice）运输。-20℃避光保存，避免反复冻融，一年有效。

【注】：如果需要在短时间内多次重复使用，可以在4℃避光保存，半年有效。

 

注意事项

1）由于细胞凋亡是一个快速的过程，建议样品在染色后1小时之内进行分析。

2） 对于贴壁细胞，消化是一个关键步骤。贴壁细胞诱导细胞凋亡时如有漂浮细胞，需收集漂浮细胞和贴壁细胞后合并染色。处理贴壁细胞时要小心操作，尽量避免人为的损伤。胰酶消化时间过短，细胞需要用力吹打才能脱落，容易造成细胞膜的损伤；PI摄入过多，消化时间过长，细胞膜同样易造成损伤，甚至会影响细胞膜上磷脂酰丝氨酸与Annexin V-FITC的结合。消化时将胰酶铺满孔板底后，轻摇使胰酶与细胞充分接触，然后倒掉大部分胰酶，利用剩余少量胰酶再消化一段时间，待细胞间空隙增大，瓶底呈花斑状即可终止。在消化液中尽量不用EDTA，EDTA会影响Annexin V与PS的结合。

3）如果样品来源于血液，请务必除去血液中的血小板。因为血小板含有PS，能与Annexin V结合，从而干扰实验结果。可以使用含有EDTA的缓冲剂并在200 g离心洗去血小板。

4）试剂在开盖前请短暂离心，将盖内壁上的液体甩至管底，避免开盖时液体洒落。

5）Annexin V-FITC和PI是光敏物质，在操作时请注意避光。

6）本产品仅作科研用途！

 

操作方法

1.1 样品染色

1）悬浮细胞：300 g，4℃离心5 min收集细胞。

贴壁细胞：用不含EDTA的胰酶消化后，300 g，4℃离心5 min收集细胞。胰酶消化时间不宜过长，以防引起假阳性。

2）用预冷的PBS洗涤细胞2次，每次均需300 g，4℃离心5 min。收集1～5×105细胞。

3）吸弃PBS，加入100 μL 1×Binding Buffer重悬细胞。

4）加入5 μL Annexin V-FITC和10 μLPI Staining Solution，轻轻混匀。

5）避光、室温反应10-15 min。

6）加入400 μL 1×Binding Buffer，混匀后放置于冰上，样品在1小时内用流式细胞仪或荧光显微镜检测。

【注】：为了避免洗涤细胞时损失细胞，在吸液时可以用大的Tip头套上小的Tip头吸液。

1.2 样品分析

A．流式细胞仪分析：

FITC最大激发波长为488 nm，最大发射波长525 nm，FITC的绿色荧光在FL1通道检测；PI-DNA复合物的最大激发波长为535 nm，最大发射波长为615 nm，PI的红色荧光在FL2或FL3通道检测。用CellQuest等软件进行分析，绘制双色散点图（two-color dot plot），FITC为横坐标，PI为纵坐标。典型的实验中，细胞可以分成三个亚群，活细胞仅有很低强度的背景荧光，早期凋亡细胞仅有较强的绿色荧光，晚期凋亡细胞有绿色和红色荧光双重染色。

B．荧光显微镜分析：

1）滴一滴用Annexin V-FITC/PI双染的细胞悬液于载玻片上，并用盖玻片盖上细胞。

【注】：对于贴壁细胞，可直接用盖玻片培养细胞并诱导细胞凋亡。

2）在荧光显微镜下用双色滤光片观察。Annexin V-FITC荧光信号呈绿色，PI荧光信号呈红色。

 

相关产品

产品名称

货号

规格

Cell Cycle and Apoptosis Analysis Kit

细胞周期与细胞凋亡检测试剂盒

40301ES50

50 T

40301ES60

100 T

Annexin V-EGFP/PI 细胞凋亡检测试剂盒

Annexin V-EGFP/PI Apoptosis Detection Kit

40303ES20

20 T

40303ES50

50 T

40303ES60

100 T

Annexin V-Alexa Fluor 647/PI 细胞凋亡检测试剂盒

Annexin V-Alexa Fluor 647/PI Apoptosis Detection Kit

40304ES20

20 T

40304ES50

50 T

40304ES60

100 T

Annexin V-Alexa Fluor 488/PI 细胞凋亡检测试剂盒

Annexin V-Alexa Fluor 488/PI Apoptosis Detection Kit

40305ES20

20 T

40305ES50

50 T

40305ES60

100 T

Annexin V-PE/7-AAD细胞凋亡检测试剂盒

Annexin V-PE/7-AAD Apoptosis Detection Kit

40310ES20

20 T

40310ES50

50 T

40310ES60

100 T

 

 

HB220609

Q：Annexin V 和 JC-1、Tunel 细胞凋亡检测的区别？

A: Annexin V 是检测细胞早期凋亡的试剂，JC-1 是检测细胞中期凋亡的试剂、Tunel 是检测细胞晚期凋亡的试剂。

Q：Annexin V 和JC-1、Tunel 细胞凋亡检测的可以应用到植物或是细菌（原核生物） 吗？

A：可以，但是需要制备原生质体，因为植物细胞或是细菌（原核生物）含有细胞壁，具体的染液使用剂量只需浸没细胞即可，染色时间对于不同细胞有一定的不同。

Q：40302ES Annexin V-FITC/PI 细胞凋亡检测试剂盒里的PI的浓度是多少呢？

A：20ug/ml。

Q:实验结果如何判断？

A：活细胞（Annexin V-/PI-）

  早期凋亡细胞（Annexin V+/PI-）

  晚期凋亡细胞和坏死细胞呈现双阳性（Annexin V+/PI+）

  裸核（Annexin V-/PI+）

Q: ​Annexin V和TUNEL有什么区别？

A：末端脱氧核苷酸转移酶 dUTP 缺口末端标记 (TUNEL) 是一种染色方法，用于识别细胞内 DNA 片段化位点——晚期细胞凋亡的标志性特征。 它使用酶末端脱氧核苷酸转移酶 (TdT) 将修饰的 dNTP（例如 dUTP）连接到片段化 DNA 链的 3'-羟基末端。 dNTPs 通常用荧光团修饰以促进量化和/或可视化。

Annexin V 染色通过结合由于细胞膜不对称性丧失而暴露在细胞外的 PS 残基来识别细胞凋亡的早期阶段。 Annexin V 通常用 FITC 等荧光团标记，以促进凋亡细胞的检测。

Annexin V-FITC/PI细胞凋亡检测试剂盒|Annexin V-FITC/PI Apoptosis Detection Kit

 

 

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重组FITC标记人FOLR1蛋白 Recombinant FITC-Labeled Human FOLR1 Protein,His-Avi Tag

发表于由

重组FITC标记人FOLR1蛋白 Recombinant FITC-Labeled Human FOLR1 Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名（Synonyms）

FR-alpha; FR alpha; FR α; FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18; Folate receptor 1; Fbp1; Folate receptor alpha;

表达区间及表达系统（Source）

FITC-Labeled Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg25-Met233.[Accession | P15328]

分子量大小（Molecular Weight）

The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 37-47 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized FITC-Labeled Human FOLR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA.

制剂（Formulation）

Supplied as 0.22 μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人FOLR1蛋白 Recombinant FITC-Labeled Human FOLR1 Protein,His-Avi Tag

暂无内容

重组FITC标记人FOLR1蛋白 Recombinant FITC-Labeled Human FOLR1 Protein,His-Avi Tag

暂无内容

 

性能参数

分子别名（Synonyms）

FR-alpha; FR alpha; FR α; FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18; Folate receptor 1; Fbp1; Folate receptor alpha;

表达区间及表达系统（Source）

FITC-Labeled Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Arg25-Met233.[Accession | P15328]

分子量大小（Molecular Weight）

The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 37-47 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

ELISA Data: Immobilized FITC-Labeled Human FOLR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 15.0ng/ml determined by ELISA.

制剂（Formulation）

Supplied as 0.22 μm filtered solution in PBS (pH 7.4).

 

储存条件

The product should be stored at -85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组FITC标记人FOLR1蛋白 Recombinant FITC-Labeled Human FOLR1 Protein,His-Avi Tag

暂无内容

重组FITC标记人FOLR1蛋白 Recombinant FITC-Labeled Human FOLR1 Protein,His-Avi Tag

暂无内容