Recombinant Murine KGF-2/FGF-10
产品编号: P6092-1mg
产品包装:1mg
选择包装
5μg 100μg 1mg
数
量
Recombinant Murine KGF-2/FGF-10(重组小鼠KGF-2/FGF-10)(P6092-1mg)
说明书下载
价格: ¥ 23581.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| P6092-5μg | Recombinant Murine KGF-2/FGF-10 | 5μg | 669.00元 |
| P6092-100μg | Recombinant Murine KGF-2/FGF-10 | 100μg | 7363.00元 |
| P6092-1mg | Recombinant Murine KGF-2/FGF-10 | 1mg | 23581.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Mouse | 14165 | O35565 | E. coli | 173aa | 19.5kDa | － |
| About this Antibody | |
| Name | Recombinant Murine KGF-2/FGF-10 (Recombinant Murine Keratinocyte Growth Factor-2/FGF-10; rMuKGF-2/FGF-10)；Recombinant Mouse KGF-2/FGF-10 ; 重组小鼠角质细胞生长因子2/成纤维细胞生长因子10 |
| Synonyms | KGF-2; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria |
| Purity | >95% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5ng/ml, corresponding to a specific activity of >2.0×106IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, 600mM NaCl, pH7.4, 1mM mercaptoethanol. |
| Endotoxin | Less than 1EU/μg of rMuKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family, which is involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may take parts in wound healing and is required for normal branching morphogenesis. Recombinant murine FGF-10 contains a 173 amino acids and it shares 93% and 90%a.a. sequence identity with human and rat FGF-10. |
| Amino Acid Sequence | QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P6092-5μg | Recombinant Murine KGF-2/FGF-10 | 5μg |
| P6092-25μg | Recombinant Murine KGF-2/FGF-10 | 25μg |
| P6092-100μg | Recombinant Murine KGF-2/FGF-10 | 100μg |
| P6092-1mg | Recombinant Murine KGF-2/FGF-10 | 1mg |
| – | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
相关产品请点击如下按钮：
细胞因子
培养液相关
使用本产品的文献：
Mouse KGF-1/FGF-7 小鼠KGF-1/FGF-7
Mouse KGF-1/FGF-7 小鼠KGF-1/FGF-7
产品说明书
FAQ
COA
已发表文献
产品描述
成纤维生长因子7（FGF-7），也称为角质形成细胞生长因子 (KGF) ，是成纤维细胞生长因子 (FGF) 家族的成员。在胚胎发育、细胞增殖和细胞分化的调控中发挥重要作用。正常分枝形态发生所必需的，对角质形成细胞有活性的生长因子。可能是正常上皮细胞增殖的主要旁分泌效应物。在大肠杆菌中过表达的重组KGF活性约为天然蛋白的10倍，可能与重组KGF未被糖基化有关。KGF，122-132AA段是KGF受体的特异性结合位点。KGF由各种来源的间质细胞分泌，与肝素有较强的亲和力，其受体KGFR属于蛋白酪氨酸激酶受体家族，其主要分布于上皮细胞，KGF与靶细胞膜上的受体KGFR特异性结合后，促使受体自身磷酸化，从而启动细胞内信号级联反应，进而发挥多种生物学功能:参与组织器官发育、促进细胞增殖及组织损伤修复等。
本品是由大肠杆菌原核表达系统制备的重组蛋白，经分离纯化、过滤除菌和冻干等步骤制成，具有高纯度、高活性、无标签及低内毒素等特点。
性能参数
|
分子别名 |
Fibroblast growth factor 7；HBGF-7 |
|
表达系统及表达区间 |
Mouse FGF-7 is expressed from E.coli with no tag . It contains Cys32-Thr194 |
|
AA Sequence |
CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT |
|
Accession |
P36363 |
|
分子量 |
Approximately 18.7 kDa. |
|
生物活性 |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
|
外观 |
无菌过滤白色冻干粉末。 |
|
纯度 |
> 96%，SDA-PAGE和 HPLC 检测 |
|
内毒素 |
<1.0 EU/μg |
|
制剂 |
Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl. |
|
复溶方法 |
建议在打开之前短暂离心，以将内容物带到底部。在无菌蒸馏水或者含有0.1% BSA的水性缓冲液中重新配制至0.1-1.0 mg/mL的浓度。储备溶液应分成等分试样，并在≤-20°C下储存。 |
储存条件
-25~-15℃保存，收到货之后有效期1年。
复溶后， 2 ~8 °C保存，30天有效期。复溶后， -25~-15℃保存，3个月有效期。
建议首次使用时将蛋白等分，避免重复冻融循环。
注意事项
1. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
Ver.CN20240305
产品描述
成纤维生长因子7（FGF-7），也称为角质形成细胞生长因子 (KGF) ，是成纤维细胞生长因子 (FGF) 家族的成员。在胚胎发育、细胞增殖和细胞分化的调控中发挥重要作用。正常分枝形态发生所必需的，对角质形成细胞有活性的生长因子。可能是正常上皮细胞增殖的主要旁分泌效应物。在大肠杆菌中过表达的重组KGF活性约为天然蛋白的10倍，可能与重组KGF未被糖基化有关。KGF，122-132AA段是KGF受体的特异性结合位点。KGF由各种来源的间质细胞分泌，与肝素有较强的亲和力，其受体KGFR属于蛋白酪氨酸激酶受体家族，其主要分布于上皮细胞，KGF与靶细胞膜上的受体KGFR特异性结合后，促使受体自身磷酸化，从而启动细胞内信号级联反应，进而发挥多种生物学功能:参与组织器官发育、促进细胞增殖及组织损伤修复等。
本品是由大肠杆菌原核表达系统制备的重组蛋白，经分离纯化、过滤除菌和冻干等步骤制成，具有高纯度、高活性、无标签及低内毒素等特点。
性能参数
|
分子别名 |
Fibroblast growth factor 7；HBGF-7 |
|
表达系统及表达区间 |
Mouse FGF-7 is expressed from E.coli with no tag . It contains Cys32-Thr194 |
|
AA Sequence |
CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT |
|
Accession |
P36363 |
|
分子量 |
Approximately 18.7 kDa. |
|
生物活性 |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
|
外观 |
无菌过滤白色冻干粉末。 |
|
纯度 |
> 96%，SDA-PAGE和 HPLC 检测 |
|
内毒素 |
<1.0 EU/μg |
|
制剂 |
Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl. |
|
复溶方法 |
建议在打开之前短暂离心，以将内容物带到底部。在无菌蒸馏水或者含有0.1% BSA的水性缓冲液中重新配制至0.1-1.0 mg/mL的浓度。储备溶液应分成等分试样，并在≤-20°C下储存。 |
储存条件
-25~-15℃保存，收到货之后有效期1年。
复溶后， 2 ~8 °C保存，30天有效期。复溶后， -25~-15℃保存，3个月有效期。
建议首次使用时将蛋白等分，避免重复冻融循环。
注意事项
1. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
Ver.CN20240305
暂无内容
暂无内容
Human FGF-2 重组人碱性成纤维细胞生长因子(人FGF-2)
Human FGF-2 重组人碱性成纤维细胞生长因子(人FGF-2)
产品说明书
FAQ
COA
已发表文献
FGF-2, also known as basic fibroblast growth factor, is a canonical FGF that belongs to the FGF-1 subfamily. It is a regulator of proliferation, migration, differentiation, cell survival, and stemness in human stem cells. More over，FGF-2 play a major role in skeletal development, bone formation, and fracture repair, which make FGF-2 an attractive molecule for clinical and pharmaceutical applications in bone regeneration.
性能参数
|
Synonyms |
bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin |
|
Uniprot No. |
P09038 |
|
Source |
E.coli-derived human FGF, Pro143-Ser288, with an N-terminal Gly. |
|
Molecular Weight |
Approximately 16.5 kDa. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
<0.1 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS. |
|
Reconstitution |
Centrifuge tubes before opening. Dissolve lyophilized protein with PBS to ensure the concentration is greater than 100 ug/mL.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. |
储存条件
The product should be stored at -25~–15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~–15℃ under sterile conditions after reconstitution.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
产品数据
Ver. CN20230607
FGF-2, also known as basic fibroblast growth factor, is a canonical FGF that belongs to the FGF-1 subfamily. It is a regulator of proliferation, migration, differentiation, cell survival, and stemness in human stem cells. More over，FGF-2 play a major role in skeletal development, bone formation, and fracture repair, which make FGF-2 an attractive molecule for clinical and pharmaceutical applications in bone regeneration.
性能参数
|
Synonyms |
bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin |
|
Uniprot No. |
P09038 |
|
Source |
E.coli-derived human FGF, Pro143-Ser288, with an N-terminal Gly. |
|
Molecular Weight |
Approximately 16.5 kDa. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
<0.1 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS. |
|
Reconstitution |
Centrifuge tubes before opening. Dissolve lyophilized protein with PBS to ensure the concentration is greater than 100 ug/mL.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. |
储存条件
The product should be stored at -25~–15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~–15℃ under sterile conditions after reconstitution.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
产品数据
Ver. CN20230607
暂无内容
暂无内容
reddotbiotech RD-FGF10-Hu说明书
Reddot Biotech Inc.位于加拿大不列颠哥伦比亚省基洛纳，由一支在医疗器械行业拥有超过35年经验的执行团队于2015年成立。我们专注于为市场的客户提供及时的客户服务，可靠的供应和具有性能的产品。我们依靠竞争，创新和快速资源分配来满足客户需求。
Reddot Biotech Inc.目前专注于销售用于制药，实验室，医学和科研用途的试剂和相关产品。我们致力于研究和开发，为客户提供高质量的产品，为任何研究提供准确，可靠和快速的结果。凭借与各大洲公司和研究机构合作的丰富经验，我们为我们提供的产品和服务提供了巨大的价值。
RD-FGF10-HuHuman Fibroblast Growth Factor 10 (FGF10) ELISA Kit
|
Catalog Number |
RD-FGF10-Hu |
|
Detection Range |
15.625-1000pg/mL |
|
Assay Length |
1-4.5 hours |
|
Method |
sandwich |
|
Sensitivity |
5.7pg/mL |
Reddotbiotech 常见问题解答
传统的ELISA试剂盒与即用型ELISA试剂盒
我们新的“即用型”ELISA试剂盒具有以下特点：
- 缩短实验时间
- 预稀释检测试剂
- 增强稳定性，允许在4 o C下储存整个试剂盒
这些试剂盒仍然保持我们高水平的质量和灵敏度，以及我们12个月的保质期。
与传统的ELISA试剂盒相比，我们的即用型试剂盒使用起来更容易，更快捷。
提供的试剂和材料
|
传统的96T ELISA试剂盒
|
即用型96T ELISA试剂盒
|
|
传统的48T ELISA试剂盒
|
即用型48T ELISA试剂盒
|
所需材料但未提供套件
- 微孔板读数器，450±10nm滤光片。
- 精密单通道或多通道移液器和一次性吸头。
- 微量离心管用于稀释。
- 去离子水或蒸馏水。
- 用于印迹微量滴定板的吸水纸。
- 稀释洗涤缓冲液的容器。
存放套件
对于未开封的套件
传统的ELISA试剂盒：所有试剂应根据样品瓶上的标签保存。的TMB底物，洗涤缓冲液（30X浓缩物）和终止溶液应在被存储4 ö Ç 在接收而其他人应在-20 Ô Ç 。
即用型ELISA试剂盒：所有试剂应在收到后于4 o C储存。
打开套件
打开试剂盒后，仍需要根据上述储存条件储存剩余的试剂。此外，将未使用的孔返回到含有干燥剂包的铝箔袋中，并沿着拉链密封的整个边缘重新密封。
注意：
有关套件的失效日期，请参阅套件盒上的标签。在此日期之前，所有组件都是稳定的
强烈建议在开封后1个月内使用剩余的试剂。
样品采集和存储
请注意您感兴趣的套件推荐哪些样品类型。
血清
使用血清分离管，让样品在室温下凝固2小时或在4℃下过夜，然后以约1000×g离心20分钟。立即测定新鲜制备的血清，或将样品分装在-20℃或-80℃下备用。避免反复冻/融循环。
等离子体
使用EDTA或肝素作为抗凝血剂收集血浆。在收集后30分钟内，在2-8℃下以1000×g离心样品15分钟。立即去除血浆并进行检测，或将样品保存在-20℃或-80℃的等分试样中以备后用。避免反复冻/融循环。
组织匀浆
组织匀浆的制备将根据组织类型而变化。通常，组织应在冰冷的PBS（0.02mol / L，pH 7.0-7.2）中*冲洗以除去过量的血液并在均质化前称重。将组织切成小块并在冰上用玻璃匀浆器在5-10mL PBS中均化（Micro Tissue Grinders也起作用）。应使用超声波细胞破碎器对所得悬浮液进行超声处理，或进行两次冻融循环以进一步破坏细胞膜。之后，匀浆应以5000×g离心5分钟。立即取出上清液并进行分析，或等分试样并在≤-20℃下储存。
细胞裂解物
根据以下说明，在测定前必须裂解细胞：
- 应用胰蛋白酶分离贴壁细胞，然后通过离心收集（悬浮细胞可通过直接离心收集）。
- 在冷PBS中洗涤细胞三次。
- 在1X PBS中重悬细胞并进行4次超声波处理（或在≤-20℃下冷冻细胞。轻轻混合解冻细胞。重复冻融循环3次。）
- 在2 – 8℃下以1500×g离心10分钟以除去细胞碎片。细胞培养上清液和其他生物液体 – 以1000×g离心20分钟。立即去除颗粒物并进行分析，或将样品分装在-20℃或-80℃的等分试样中。避免反复冻/融循环。
唾液
使用收集装置或等同物收集唾液。在2-8在1000×g下离心15分钟样品ö C.在≤-20删除在等分试样的微粒，并立即检测，或将ö C.避免反复冷冻/解冻循环。
尿
无菌收集当天的批尿液（中游），直接收集到无菌容器中。离心机以除去颗粒物质，测定立即或等分试样并储存在≤-20 ö C.避免反复冻融。
牛奶
用于以2 10,000×G下离心15分钟的样品- 8 ø C.收集总共3个周期的水性馏分和离心机两次以上。立即测定或将样品分装在-20 o C或-80 o C以备后用。避免反复冻/融循环。
泪液，细胞培养上清液和其他生物液体
以1000×g离心样品20分钟。立即去除颗粒物并进行检测，或将样品保存在-20 o C或-80 o C的等分试样中。避免反复冻/融循环。
注意：
- 样品在5天内可以被存储在4使用Ô C，否则样品必须储存在-20 Ô C（≤1月）或-80 Ô C（≤2个月），以避免生物活性和/或污染的损失。
- 样品溶血会影响结果; 不会检测到用作样本的溶血标本。
- 进行测定时，将样品置于室温下。
试剂准备
*使用前将所有试剂盒组分和样品置于室温（18-25 o C）。
标准
用标准稀释剂重新配制标准品，在室温下保持10分钟，轻轻摇动（不要起泡）。有关储备溶液中标准浓度的信息，请参阅手册。准备7管含有0.5mL标准稀释剂的试管，并使用稀释的标准品生产双稀释系列。在下次转移之前*混合每个管。
检测试剂A和检测试剂B.
传统的ELISA试剂盒：使用前短暂旋转或离心检测A和检测B溶液。分别用Assay Diluent A和B稀释至工作浓度（1：100）。
即用型ELISA试剂盒：检测溶液A和B的浓度已经正确，无需进一步稀释。
洗液
用580mL去离子水或蒸馏水稀释20mL洗涤液浓缩物（30×），制备600mL洗涤液（1×）。
TMB底物
用灭菌的吸头吸取所需剂量的溶液。不要将残留的溶液倒回到小瓶中。
注意：
- 不要直接在孔中进行连续稀释。
- 在进行测定后15分钟内制备标准品。不要直接在37 o C 溶解试剂。
- 检测试剂A和B是粘性溶液，因此慢慢移液它们以减少体积误差。
- 传统的ELISA试剂盒：根据说明小心地重新构建标准品或使用检测试剂A和B，避免起泡并轻轻混合直至晶体*溶解。为了大限度地减少移液造成的不性，请使用小体积并确保移液器已校准。建议一次移液10μL以上以确保准确性。
- 即用型ELISA试剂盒：重组标准品只能使用一次。
- 重构的标准品，检测试剂A和检测试剂B只能使用一次。
- 如果在洗涤液浓缩物（30×）中形成晶体，则温热至室温并轻轻混合直至晶体*溶解。
- 试剂制备过程中使用的任何污染水或容器都会影响检测结果。
样品制备
- Reddot Biotech仅对试剂盒本身负责，而不是对试验过程中消耗的样品负责。实验者应该在开始实验之前总是计算所需的样品总量。
- 实验者还应该在开始之前预测大致的浓度。如果预期值不在标准曲线范围内，建议确定样品的宜稀释度。
- 如果您使用的样品类型未在手册中指出，则应在开始实际实验之前进行初步实验以确保试剂盒的有效性。
- 建议不要使用化学裂解缓冲液来制备组织或细胞提取样品，因为它可能会由于裂解缓冲液的含量而导致意外的反应和结果。
- 由于其他来源的抗原与我们试剂盒中使用的抗体之间可能存在错配，因此不建议使用其他制造商生产的天然或重组蛋白，因为我们的试剂盒可能无法识别它们。
- 我们的试剂盒可能无法检测到细胞培养上清液中的样品，因为它们很容易受到细胞活力，细胞数量和/或取样时间等因素的影响。
- 建议将未经长期储存的新鲜样品与我们的试剂盒一起使用。如果样品长时间未冷冻保存，可能会发生蛋白质降解和变性，导致结果相互矛盾或不正确。
分析程序
用于夹心ELISA试剂盒
- 确定稀释标准品，空白样品和样品的孔。为标准准备7口井，为空白准备1口井。每种稀释标准品（读取试剂制备），空白和样品加入100μL到适当的孔中。用Plate密封剂盖住。在37 o C 孵育2小时。
- 从每个井中取出液体，不要洗。
- 向每个孔中加入100μL检测试剂A工作溶液（即用型试剂盒中的检测溶液A）。用Plate密封剂覆盖后，在37 o C 孵育1小时。
- 吸出溶液，用喷枪，多通道移液器，歧管分配器或自动洗衣机在每个孔中用350μL1×洗涤液洗涤，静置1~2分钟。通过将板轻敲到吸水纸上，*从所有孔中除去剩余的液体。*洗净3次。后一次洗涤后，通过抽吸或倾析除去任何剩余的洗涤缓冲液。翻转平板并将其吸附在吸水纸上。
- 向每个孔中加入100μL检测试剂B工作溶液（即用型试剂盒中的检测溶液B）。用Plate密封剂覆盖后，在37 o C 孵育1小时。
- 如步骤4中所述，重复抽吸/洗涤过程总共5次。
- 每孔加入90μL底物溶液。盖上新的Plate密封剂。在37 o C 孵育15-25分钟（不要超过30分钟）。避光。加入底物溶液后液体会变蓝。
- 每孔加入50μL终止液。加入Stop溶液后液体会变黄。通过敲击板的侧面来混合液体。如果颜色变化不均匀，请轻轻敲打板以确保充分混合。
- 去除板底部的任何水滴和指纹，确认液体表面没有气泡。运行酶标仪，立即进行450nm的测量。
适用于竞争性ELISA试剂盒
- 确定稀释标准品，空白样品和样品的孔。为标准准备7口井，为空白准备1口井。分别加入50μL标准稀释液（读取试剂制备），空白和样品加入适当的孔中。然后立即向每个孔中加入50μL检测试剂A（即用型试剂盒中的检测溶液A）。轻轻摇动平板（建议使用微孔板振荡器）。用Plate密封剂覆盖。在37 o C 孵育1小时。检测试剂A可能会出现混浊。温热至室温并轻轻混合直至溶液看起来均匀。
- 吸出溶液，用喷枪，多通道移液器，歧管分配器或自动洗涤器，每孔加入350μL1×洗涤液，静置1-2分钟。通过将板轻敲到吸水纸上，*从所有孔中除去剩余的液体。*洗净3次。后一次洗涤后，通过抽吸或倾析除去任何剩余的洗涤缓冲液。翻转平板并将其吸附在吸水纸上。
- 向每个孔中加入100μL检测试剂B工作溶液（即用型试剂盒中的检测溶液B）。用Plate密封剂覆盖后，在37 o C 孵育1小时。
- 在步骤2中重复抽吸/洗涤过程总共5次。
- 每孔加入90μL底物溶液。盖上新的Plate密封剂。在37 o C 孵育15-25分钟（不要超过30分钟）。避光。加入底物溶液后液体会变蓝。
- 每孔加入50μL终止液。加入Stop溶液后液体会变黄。通过敲击板的侧面来混合液体。如果颜色变化不均匀，请轻轻敲打板以确保充分混合。
- 去除板底部的任何水滴和指纹，确认液体表面没有气泡。运行酶标仪，立即进行450nm的测量。
注意：
- 分析准备：为每个实验保留适当数量的孔，并从微孔板中移除额外的孔。剩余的井应重新密封，并保存在-20 o C的传统套件中，4 o C的即用型套件。
- 样品或试剂添加：请使用新制备的标准品。小心地将样品加入孔中并轻轻混合以避免起泡。不要触摸井壁。对于该程序中的每个步骤，将试剂或样品添加到测定板的总分配时间不应超过10分钟。这将确保每个移液步骤的相等的经过时间，而不会中断。建议复制所有标准品和标本，尽管不是必需的。为避免交叉污染，在添加标准品，样品和试剂之间更换移液器吸头。此外，对每种试剂使用分离的储库。
- 孵育：为了确保准确的结果，需要在孵育步骤中适当粘附板密封剂。在孵化步骤之间不允许孔长时间未被覆盖。一旦将试剂添加到孔条中，在测定期间不要让条带干燥。必须控制孵育时间和温度。
- 洗涤：洗涤程序至关重要。在每个步骤中*去除液体对于试剂盒的良好性能是*的。后一次清洗后，通过抽吸或倾析除去任何残留的清洗液，并去除板底部的任何水滴或指纹。洗涤不足将导致精度差和吸光度读数错误升高。
- 控制反应时间：观察加入TMB底物后的颜色变化（例如每10分钟观察一次），如果颜色太深，则提前加入终止溶液以避免过强的反应，这将导致吸光度读数不准确。
- TMB底物 很容易被污染。保护它免受光线和物理污染。
- 环境湿度可能对从试剂盒获得的结果产生影响。如果您工厂的湿度低于60％，建议使用加湿器。
测试原理
用于夹心ELISA试剂盒
该试剂盒中提供的微量滴定板预先涂有对靶抗原特异的抗体。然后将标准品或样品加入适当的微量滴定板孔中，用对靶抗原特异的生物素缀合的抗体制剂。接下来，将与辣根过氧化物酶（HRP）缀合的抗生物素蛋白加入每个微孔板孔中并孵育。在添加TMB底物溶液后，仅含有靶抗原，生物素缀合的抗体和酶缀合的抗生物素蛋白的孔将显示颜色变化。通过添加硫酸溶液终止酶 – 底物反应，并且在450nm±10nm的波长下通过分光光度法测量颜色变化。然后通过比较样品的OD与标准曲线确定样品中靶抗原的浓度。
适用于竞争性ELISA试剂盒
该测定采用竞争性抑制酶免疫测定技术。将靶抗原特异性的单克隆抗体预先包被在微孔板上。在生物素标记的靶抗原和未标记的靶抗原（标准品或样品）之间启动竞争性抑制反应，其中预涂的抗体对靶抗原具有特异性。温育后，洗去未结合的缀合物。接下来，将与辣根过氧化物酶（HRP）缀合的抗生物素蛋白加入每个微孔板孔中并孵育。结合的HRP缀合物的量是反向正比于样品中的靶抗原的浓度。加入底物溶液后，显色的强度与样品中靶抗原的浓度成比例。
结果计算
用于夹心ELISA试剂盒
平均每个标准品，对照品和样品的重复读数，然后减去平均零标准光密度。通过绘制每个标准品的平均OD和浓度构建标准曲线，并通过图表上的点绘制宜拟合曲线，或在log-log图纸上创建标准曲线，其中y轴上的目标抗原浓度和吸光度x轴。还建议使用绘图软件（例如，曲线专家1.30）。如果样品已稀释，则从标准曲线读取的浓度必须乘以稀释倍数。
适用于竞争性ELISA试剂盒
该测定采用竞争性抑制酶免疫测定技术，因此样品中的靶抗原浓度与测定信号强度之间存在负相关。平均每个标准品，对照品和样品的重复读数。在对数 – 对数或半对数图纸上创建标准曲线，在y轴上记录目标抗原浓度，在x轴上记录吸光度。通过标准点绘制宜拟合直线，或者可以通过回归分析确定。还建议使用绘图软件（例如，曲线专家1.30）。如果样品已稀释，则从标准曲线读取的浓度必须乘以稀释倍数。
灵敏度
该测定的灵敏度或检测下限（LLD）被定义为可以与零区分的低蛋白质浓度。通过将两个标准偏差加到二十个零标准重复的平均光密度值并计算相应的浓度来确定。
夹心ELISA试剂盒的典型数据
为了使计算更容易，我们绘制标准的OD值（X轴）与标准的已知浓度（Y轴），尽管浓度是自变量，OD值是因变量。然而，标准曲线的OD值可以根据测定性能的条件（例如操作者，移液技术，洗涤技术或温度效应）而变化，建议绘制数据的对数以建立每个测试的标准曲线。有关典型的标准曲线，请参阅套件中包含的手册。
确定所有套件精度的一般方法如下所述。可根据要求提供个人批量套件的规格。
- 测定内精密度（测定内的度）：分别在一个平板上测试3个具有低，中和高水平指数的样品20次。
- 批间精密度（测定之间的度）：在3个不同的平板上测试3个具有低，中和高水平指数的样品，每个平板8个重复。
- CV（％）= SD /平均值（X）100
- 分析内：CV <10％
- Inter-Assay：CV <12％
稳定性
我们的ELISA试剂盒的稳定性取决于活性丧失的速率。在适当的储存条件下，我们的试剂盒的失效率在失效日期内通常低于5％。
为尽量减少外部对我们套件性能的影响，应始终控制操作程序和实验室条件。需要特别注意的因素包括：室温，空气湿度和培养箱温度。还强烈建议整个测定由相同的实验者从开始到结束进行。
分析程序摘要
用于夹心ELISA试剂盒
- 准备所有试剂，样品和标准品;
- 每孔加入100μL标准品或样品。在37 o C 孵育2小时;
- 吸出并加入100μL制备的检测试剂A（即用型试剂盒中的检测溶液A）。在37 o C 孵育1小时;
- 吸3次洗净;
- 加入100μL制备的检测试剂B（即用型试剂盒中的检测溶液B）。在37 o C 孵育1小时;
- 吸取并洗涤5次;
- 加入90μL底物溶液。在37 o C 孵育15-25分钟;
- 加入50μL终止液。立即读取450nm。
适用于竞争性ELISA试剂盒
- 准备所有试剂，样品和标准品;
- 每孔加入50μL标准品或样品。然后立即加入50μL制备的检测试剂A（即用型试剂盒中的检测溶液A）。摇晃和混合。在37oC孵育1小时;
- 吸3次洗净;
- 加入100μL制备的检测试剂B（即用型试剂盒中的检测溶液B）。在37oC孵育1小时;
- 吸取并洗涤5次;
- 加入90μL底物溶液。在37oC孵育15-25分钟;
- 加入50μL终止液。立即读取450nm。
重要的提示
- 受现有条件和科学技术的限制，不可能对供应商提供的原材料进行全面的鉴定和分析测试。因此，可能存在一些定性和/或技术风险。
- 终的实验结果将与产品的有效性，终用户的操作技能和实验环境密切相关。请确保有足够的样本可以获得准确的结果。
- 不同批次的试剂盒在检测范围，灵敏度和显色时间上可能略有不同。请*按照套件中包含的使用说明书进行实验。我们的电子版仅供参考。
- 不要将试剂从一个试剂盒中混合或替换为另一个试剂盒。仅使用制造商提供的试剂。
- 在储存和孵育期间保护所有试剂免受强光照射。所有试剂瓶盖应紧密关闭，以防止液体蒸发和微生物污染。
- 当次打开板时，孔中可能存在雾状物质。它对终的测定结果没有任何影响。在需要之前，不要从储存袋中取出微量滴定板。
- 试剂准备和装载过程中的程序不正确，以及读板器的参数设置不正确可能导致错误的结果。在450±10nm波长下带宽为10nm或更小且光密度范围为0-3OD或更大的微孔板读数器可用于吸光度测量。请仔细阅读说明书并在实验前调整仪器。
- 即使是同一个实验者也可能从两个独立的实验中获得不同的结果。为了获得更好的可重复结果，应该控制测定中每个步骤的操作。此外，建议在每批次的一般测定之前进行初步实验。
- 每个套件都经过了几次严格的质量控制测试。但是，由于某些意外的运输条件或不同的实验室设备，终用户的结果可能与我们的内部数据不一致。不同批次的试剂盒之间的测定内差异也可能来自上述因素。
- 来自不同制造商的相同产品的套件可能会产生不同的结果，因为我们没有将我们的产品与其他制造商进行比较。
- 该试剂盒中的标准品以及抗体制备中使用的抗原通常是重组蛋白质。不同表达的序列，表达系统和/或纯化方法可用于制备重组蛋白。在我们的试剂盒中，抗体和抗体对的筛选技术也存在差异。因此，我们无法保证我们的试剂盒能够检测其他公司生产的重组蛋白。我们不建议使用Reddot Biotech ELISA试剂盒检测其他重组蛋白。
- 有效期：12个月。
- 说明手册也适用于48T套件，但48T套件中的所有试剂都减少了一半。
预防
提供与该试剂盒一起使用的终止溶液是酸溶液。使用此试剂时，请佩戴眼睛，手，脸和衣物。
|
货号 |
品名 |
规格 |
品牌 |
|
RD-ACE2-Ra |
Rat Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit |
96T |
reddotbiotech |
|
RD-ACE2-Ra |
Rat Angiotensin I Converting Enzyme 2 (ACE2) ELISA Kit |
48T |
reddotbiotech |
|
RD-ACVAB-Hu |
Human Activin AB (ACVAB) ELISA Kit |
96T |
reddotbiotech |
|
RD-ACVAB-Hu |
Human Activin AB (ACVAB) ELISA Kit |
48T |
reddotbiotech |
|
RD-ACVA-Hu |
Human Activin A (ACVA) ELISA Kit |
96T |
reddotbiotech |
|
RD-ACVA-Hu |
Human Activin A (ACVA) ELISA Kit |
48T |
reddotbiotech |
|
RD-ACVA-Mu |
Mouse Activin A (ACVA) ELISA Kit |
96T |
reddotbiotech |
|
RD-ACVA-Mu |
Mouse Activin A (ACVA) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADIPOR1-Ra |
Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADIPOR1-Ra |
Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADIPOR2-Hu |
Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADIPOR2-Hu |
Human Adiponectin Receptor 2 (ADIPOR2) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADIPOR2-Ra |
Rat Adiponectin Receptor 2 (ADIPOR2) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADIPOR2-Ra |
Rat Adiponectin Receptor 2 (ADIPOR2) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADP-b |
Bovine Adiponectin (ADP) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADP-b |
Bovine Adiponectin (ADP) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADP-c |
Canine Adiponectin (ADP) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADP-c |
Canine Adiponectin (ADP) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADP-Ch |
Chicken Adiponectin (ADP) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADP-Ch |
Chicken Adiponectin (ADP) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADP-Hu |
Human Adiponectin (ADP) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADP-Hu |
Human Adiponectin (ADP) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADP-Mu |
Mouse Adiponectin (ADP) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADP-Mu |
Mouse Adiponectin (ADP) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADP-p |
Porcine Adiponectin (ADP) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADP-p |
Porcine Adiponectin (ADP) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADP-Ra |
Rat Adiponectin (ADP) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADP-Ra |
Rat Adiponectin (ADP) ELISA Kit |
48T |
reddotbiotech |
|
RD-ADP-Rb |
Rabbit Adiponectin (ADP) ELISA Kit |
96T |
reddotbiotech |
|
RD-ADP-Rb |
Rabbit Adiponectin (ADP) ELISA Kit |
48T |
reddotbiotech |
|
RD-AGT-Hu |
Human Angiotensinogen (AGT) ELISA Kit |
96T |
reddotbiotech |
|
RD-AGT-Hu |
Human Angiotensinogen (AGT) ELISA Kit |
48T |
reddotbiotech |
|
RD-AGT-Mu |
Mouse Angiotensinogen (AGT) ELISA Kit |
96T |
reddotbiotech |
|
RD-AGT-Mu |
Mouse Angiotensinogen (AGT) ELISA Kit |
48T |
reddotbiotech |
|
RD-AGTR1-Hu |
Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit |
96T |
reddotbiotech |
|
RD-AGTR1-Hu |
Human Angiotensin II Receptor 1 (AGTR1) ELISA Kit |
48T |
reddotbiotech |
|
RD-AGTR1-Ra |
Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit |
96T |
reddotbiotech |
|
RD-AGTR1-Ra |
Rat Angiotensin II Receptor 1 (AGTR1) ELISA Kit |
48T |
reddotbiotech |
|
RD-AGTR2-Hu |
Human Angiotensin II Receptor 2 (AGTR2) ELISA Kit |
96T |
reddotbiotech |
|
RD-AGTR2-Hu |
Human Angiotensin II Receptor 2 (AGTR2) ELISA Kit |
48T |
reddotbiotech |
|
RD-AGTR2-Ra |
Rat Angiotensin II Receptor 2 (AGTR2) ELISA Kit |
96T |
reddotbiotech |
|
RD-AGTR2-Ra |
Rat Angiotensin II Receptor 2 (AGTR2) ELISA Kit |
48T |
reddotbiotech |
|
RD-AGT-Ra |
Rat Angiotensinogen (AGT) ELISA Kit |
96T |
reddotbiotech |
|
RD-AGT-Ra |
Rat Angiotensinogen (AGT) ELISA Kit |
48T |
reddotbiotech |
|
RD-ALCAM-Hu |
Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit |
96T |
reddotbiotech |
|
RD-ALCAM-Hu |
Human Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit |
48T |
reddotbiotech |
|
RD-ALCAM-Mu |
Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit |
96T |
reddotbiotech |
|
RD-ALCAM-Mu |
Mouse Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit |
48T |
reddotbiotech |
|
RD-ALCAM-Ra |
Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit |
96T |
reddotbiotech |
|
RD-ALCAM-Ra |
Rat Activated Leukocyte Cell Adhesion Molecule (ALCAM) ELISA Kit |
48T |
reddotbiotech |
|
RD-Ang1-7-c |
Canine Angiotensin 1-7 (Ang1-7) ELISA Kit |
96T |
reddotbiotech |
|
RD-Ang1-7-c |
Canine Angiotensin 1-7 (Ang1-7) ELISA Kit |
48T |
reddotbiotech |
|
RD-Ang1-7-Hu |
Human Angiotensin 1-7 (Ang1-7) ELISA Kit |
96T |
reddotbiotech |
|
RD-Ang1-7-Hu |
Human Angiotensin 1-7 (Ang1-7) ELISA Kit |
48T |
reddotbiotech |
|
RD-Ang1-7-Mu |
Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit |
96T |
reddotbiotech |
|
RD-Ang1-7-Mu |
Mouse Angiotensin 1-7 (Ang1-7) ELISA Kit |
48T |
reddotbiotech |
|
RD-Ang1-7-Ra |
Rat Angiotensin 1-7 (Ang1-7) ELISA Kit |
96T |
reddotbiotech |
|
RD-Ang1-7-Ra |
Rat Angiotensin 1-7 (Ang1-7) ELISA Kit |
48T |
reddotbiotech |
|
RD-Ang1-9-Hu |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
96T |
reddotbiotech |
|
RD-Ang1-9-Hu |
Human Angiotensin 1-9 (Ang1-9) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngI-c |
Canine Angiotensin I (AngI) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngI-c |
Canine Angiotensin I (AngI) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngI-Hu |
Human Angiotensin I (AngI) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngI-Hu |
Human Angiotensin I (AngI) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngII-b |
Bovine Angiotensin II (AngII) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngII-b |
Bovine Angiotensin II (AngII) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngII-Hu |
Human Angiotensin II (AngII) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngII-Hu |
Human Angiotensin II (AngII) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngIII-Hu |
Human Angiotensin III (AngIII) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngIII-Hu |
Human Angiotensin III (AngIII) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngII-Mu |
Mouse Angiotensin II (AngII) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngII-Mu |
Mouse Angiotensin II (AngII) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngII-Ra |
Rat Angiotensin II (AngII) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngII-Ra |
Rat Angiotensin II (AngII) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngII-Rb |
Rabbit Angiotensin II (AngII) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngII-Rb |
Rabbit Angiotensin II (AngII) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngI-Mu |
Mouse Angiotensin I (AngI) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngI-Mu |
Mouse Angiotensin I (AngI) ELISA Kit |
48T |
reddotbiotech |
|
RD-AngI-Ra |
Rat Angiotensin I (AngI) ELISA Kit |
96T |
reddotbiotech |
|
RD-AngI-Ra |
Rat Angiotensin I (AngI) ELISA Kit |
48T |
reddotbiotech |
|
RD-ATP4a-Hu |
Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit |
96T |
reddotbiotech |
|
RD-ATP4a-Hu |
Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit |
48T |
reddotbiotech |
|
RD-ATP4a-Mu |
Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit |
96T |
reddotbiotech |
|
RD-ATP4a-Mu |
Mouse ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit |
48T |
reddotbiotech |
|
RD-ATP4a-Ra |
Rat ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit |
96T |
reddotbiotech |
|
RD-ATP4a-Ra |
Rat ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit |
48T |
reddotbiotech |
|
RD-Bcl11A-Hu |
Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit |
96T |
reddotbiotech |
|
RD-Bcl11A-Hu |
Human B-Cell CLL/Lymphoma 11A (Bcl11A) ELISA Kit |
48T |
reddotbiotech |
|
RD-Bcl2-b |
Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit |
96T |
reddotbiotech |
|
RD-Bcl2-b |
Bovine B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit |
48T |
reddotbiotech |
|
RD-Bcl2-Hu |
Human B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit |
96T |
reddotbiotech |
|
RD-Bcl2-Hu |
Human B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit |
48T |
reddotbiotech |
|
RD-Bcl2L2-Ra |
Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit |
96T |
reddotbiotech |
|
RD-Bcl2L2-Ra |
Rat B-Cell CLL/Lymphoma 2 Like Protein 2 (Bcl2L2) ELISA Kit |
48T |
reddotbiotech |
|
RD-Bcl2L-Mu |
Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit |
96T |
reddotbiotech |
|
RD-Bcl2L-Mu |
Mouse B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) ELISA Kit |
48T |
reddotbiotech |
|
RD-Bcl2-Mu |
Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit |
96T |
reddotbiotech |
|
RD-Bcl2-Mu |
Mouse B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit |
48T |
reddotbiotech |
|
RD-Bcl2-Ra |
Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit |
96T |
reddotbiotech |
|
RD-Bcl2-Ra |
Rat B-Cell Leukemia/Lymphoma 2 (Bcl2) ELISA Kit |
48T |
reddotbiotech |
|
RD-Bcl9-Mu |
Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit |
96T |
reddotbiotech |
|
RD-Bcl9-Mu |
Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit |
48T |
reddotbiotech |
|
RD-BDNF-c |
Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
96T |
reddotbiotech |
|
RD-BDNF-c |
Canine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
48T |
reddotbiotech |
|
RD-BDNF-Ch |
Chicken Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
96T |
reddotbiotech |
|
RD-BDNF-Ch |
Chicken Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
48T |
reddotbiotech |
|
RD-BDNF-Gu |
Guinea pig Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
96T |
reddotbiotech |
|
RD-BDNF-Gu |
Guinea pig Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
48T |
reddotbiotech |
|
RD-BDNF-Hu |
Human Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
96T |
reddotbiotech |
|
RD-BDNF-Hu |
Human Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
48T |
reddotbiotech |
|
RD-BDNF-Mu |
Mouse Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
96T |
reddotbiotech |
|
RD-BDNF-Mu |
Mouse Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
48T |
reddotbiotech |
|
RD-BDNF-p |
Porcine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
96T |
reddotbiotech |
|
RD-BDNF-p |
Porcine Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
48T |
reddotbiotech |
|
RD-BDNF-Ra |
Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
96T |
reddotbiotech |
|
RD-BDNF-Ra |
Rat Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
48T |
reddotbiotech |
|
RD-BDNF-Rb |
Rabbit Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
96T |
reddotbiotech |
|
RD-BDNF-Rb |
Rabbit Brain Derived Neurotrophic Factor (BDNF) ELISA Kit |
48T |
reddotbiotech |
|
RD-BLC1-Hu |
Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit |
96T |
reddotbiotech |
|
RD-BLC1-Hu |
Human B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit |
48T |
reddotbiotech |
|
RD-BLC1-Mu |
Mouse B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit |
96T |
reddotbiotech |
|
RD-BLC1-Mu |
Mouse B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit |
48T |
reddotbiotech |
|
RD-BLC1-Ra |
Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit |
96T |
reddotbiotech |
|
RD-BLC1-Ra |
Rat B-Lymphocyte Chemoattractant 1 (BLC1) ELISA Kit |
48T |
reddotbiotech |
|
RD-BLK-Hu |
Human B-Lymphoid Tyrosine Kinase (BLK) ELISA Kit |
96T |
reddotbiotech |
|
RD-BLK-Hu |
Human B-Lymphoid Tyrosine Kinase (BLK) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP2-b |
Bovine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP2-b |
Bovine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP2-Ch |
Chicken Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP2-Ch |
Chicken Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP2-Hu |
Human Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP2-Hu |
Human Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP2-Mu |
Mouse Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP2-Mu |
Mouse Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP2-p |
Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP2-p |
Porcine Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP2-Ra |
Rat Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP2-Ra |
Rat Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP2-Rb |
Rabbit Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP2-Rb |
Rabbit Bone Morphogenetic Protein 2 (BMP2) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP4-b |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP4-b |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP4-c |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP4-c |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP4-Ch |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP4-Ch |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP4-g |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP4-g |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP4-Hu |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP4-Hu |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP4-Mu |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP4-Mu |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP4-p |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP4-p |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP4-Ra |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP4-Ra |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMP4-Rb |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMP4-Rb |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMPR1A-Hu |
Human Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMPR1A-Hu |
Human Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMPR1A-Mu |
Mouse Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMPR1A-Mu |
Mouse Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit |
48T |
reddotbiotech |
|
RD-BMPR1A-Ra |
Rat Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit |
96T |
reddotbiotech |
|
RD-BMPR1A-Ra |
Rat Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit |
48T |
reddotbiotech |
|
RD-C1QTNF1-Hu |
Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit |
96T |
reddotbiotech |
|
RD-C1QTNF1-Hu |
Human C1q And Tumor Necrosis Factor Related Protein 1 (C1QTNF1) ELISA Kit |
48T |
reddotbiotech |
|
RD-C1QTNF3-Hu |
Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit |
96T |
reddotbiotech |
|
RD-C1QTNF3-Hu |
Human C1q And Tumor Necrosis Factor Related Protein 3 (C1QTNF3) ELISA Kit |
48T |
reddotbiotech |
|
RD-C1QTNF9-Hu |
Human C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) ELISA Kit |
96T |
reddotbiotech |
|
RD-C1QTNF9-Hu |
Human C1q And Tumor Necrosis Factor Related Protein 9 (C1QTNF9) ELISA Kit |
48T |
reddotbiotech |
|
RD-cAMP-Ge |
General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit |
96T |
reddotbiotech |
|
RD-cAMP-Ge |
General Cyclic Adenosine Monophosphate (cAMP) ELISA Kit |
48T |
reddotbiotech |
|
RD-CSF2Rb-Hu |
Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit |
96T |
reddotbiotech |
|
RD-CSF2Rb-Hu |
Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit |
48T |
reddotbiotech |
|
RD-CTGF-b |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-CTGF-b |
Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-CTGF-Hu |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-CTGF-Hu |
Human Connective Tissue Growth Factor (CTGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-CTGF-Mu |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-CTGF-Mu |
Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-CTGF-p |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-CTGF-p |
Porcine Connective Tissue Growth Factor (CTGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-CTGF-Ra |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-CTGF-Ra |
Rat Connective Tissue Growth Factor (CTGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-CTGF-Rb |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-CTGF-Rb |
Rabbit Connective Tissue Growth Factor (CTGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-CTLA4-Hu |
Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit |
96T |
reddotbiotech |
|
RD-CTLA4-Hu |
Human Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) ELISA Kit |
48T |
reddotbiotech |
|
RD-EDN1-c |
Canine Endothelin 1 (EDN1) ELISA KitRD-EDN1-c |
96T |
reddotbiotech |
|
RD-EDN1-c |
Canine Endothelin 1 (EDN1) ELISA KitRD-EDN1-c |
48T |
reddotbiotech |
|
RD-EDN1-Gu |
Guinea pig Endothelin 1 (EDN1) ELISA Kit |
96T |
reddotbiotech |
|
RD-EDN1-Gu |
Guinea pig Endothelin 1 (EDN1) ELISA Kit |
48T |
reddotbiotech |
|
RD-EDN1-Hu |
Human Endothelin 1 (EDN1) ELISA Kit |
96T |
reddotbiotech |
|
RD-EDN1-Hu |
Human Endothelin 1 (EDN1) ELISA Kit |
48T |
reddotbiotech |
|
RD-EDN1-Mu |
Mouse Endothelin 1 (EDN1) ELISA Kit |
96T |
reddotbiotech |
|
RD-EDN1-Mu |
Mouse Endothelin 1 (EDN1) ELISA Kit |
48T |
reddotbiotech |
|
RD-EDN1-p |
Porcine Endothelin 1 (EDN1) ELISA Kit |
96T |
reddotbiotech |
|
RD-EDN1-p |
Porcine Endothelin 1 (EDN1) ELISA Kit |
48T |
reddotbiotech |
|
RD-EDN1-Ra |
Rat Endothelin 1 (EDN1) ELISA Kit |
96T |
reddotbiotech |
|
RD-EDN1-Ra |
Rat Endothelin 1 (EDN1) ELISA Kit |
48T |
reddotbiotech |
|
RD-EDN1-Rb |
Rabbit Endothelin 1 (EDN1) ELISA Kit |
96T |
reddotbiotech |
|
RD-EDN1-Rb |
Rabbit Endothelin 1 (EDN1) ELISA Kit |
48T |
reddotbiotech |
|
RD-EDN2-Hu |
Human Endothelin 2 (EDN2) ELISA Kit |
96T |
reddotbiotech |
|
RD-EDN2-Hu |
Human Endothelin 2 (EDN2) ELISA Kit |
48T |
reddotbiotech |
|
RD-EDN2-Ra |
Rat Endothelin 2 (EDN2) ELISA Kit |
96T |
reddotbiotech |
|
RD-EDN2-Ra |
Rat Endothelin 2 (EDN2) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGF-Hu |
Human Epidermal Growth Factor (EGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGF-Hu |
Human Epidermal Growth Factor (EGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGF-Mu |
Mouse Epidermal Growth Factor (EGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGF-Mu |
Mouse Epidermal Growth Factor (EGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGF-p |
Porcine Epidermal Growth Factor (EGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGF-p |
Porcine Epidermal Growth Factor (EGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGFR2-Hu |
Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGFR2-Hu |
Human Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGFR2-Mu |
Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGFR2-Mu |
Mouse Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGFR2-Ra |
Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGFR2-Ra |
Rat Epidermal Growth Factor Receptor 2 (EGFR2) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGF-Ra |
Rat Epidermal Growth Factor (EGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGF-Ra |
Rat Epidermal Growth Factor (EGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGFR-Hu |
Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGFR-Hu |
Human Epidermal Growth Factor Receptor (EGFR) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGFR-Mu |
Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGFR-Mu |
Mouse Epidermal Growth Factor Receptor (EGFR) ELISA Kit |
48T |
reddotbiotech |
|
RD-EGFR-Ra |
Rat Epidermal Growth Factor Receptor (EGFR) ELISA Kit |
96T |
reddotbiotech |
|
RD-EGFR-Ra |
Rat Epidermal Growth Factor Receptor (EGFR) ELISA Kit |
48T |
reddotbiotech |
|
RD-EG-VEGF-b |
Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-EG-VEGF-b |
Bovine Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-EG-VEGF-Hu |
Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-EG-VEGF-Hu |
Human Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-EG-VEGF-Mu |
Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-EG-VEGF-Mu |
Mouse Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-EG-VEGF-Ra |
Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
96T |
reddotbiotech |
|
RD-EG-VEGF-Ra |
Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit |
48T |
reddotbiotech |
|
RD-FGF10-Hu |
Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit |
96T |
reddotbiotech |
|
RD-FGF10-Hu |
Human Fibroblast Growth Factor 10 (FGF10) ELISA Kit |
48T |
reddotbiotech |
|
RD-FGF10-Mu |
Mouse Fibroblast Growth Factor 10 (FGF10) ELISA Kit |
96T |
reddotbiotech |
|
RD-FGF10-Mu |
Mouse Fibroblast Growth Factor 10 (FGF10) ELISA Kit |
48T |
reddotbiotech |
|
RD-FGF10-Ra |
Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit |
96T |
reddotbiotech |
|
RD-FGF10-Ra |
Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit |
48T |
reddotbiotech |
|
RD-FGF11-Hu |
Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit |
96T |
reddotbiotech |
|
RD-FGF11-Hu |
Human Fibroblast Growth Factor 11 (FGF11) ELISA Kit |
48T |
reddotbiotech |
|
RD-FGF11-Mu |
Mouse Fibroblast Growth Factor 11 (FGF11) ELISA Kit |
96T |
reddotbiotech |
|
RD-FGF11-Mu |
Mouse Fibroblast Growth Factor 11 (FGF11) ELISA Kit |
48T |
reddotbiotech |
|
RD-FGF11-Ra |
Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit |
96T |
reddotbiotech |
|
RD-FGF11-Ra |
Rat Fibroblast Growth Factor 11 (FGF11) ELISA Kit |
48T |
reddotbiotech |
|
RD-FGF12-Hu |
Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit |
96T |
reddotbiotech |
|
RD-FGF12-Hu |
Human Fibroblast Growth Factor 12 (FGF12) ELISA Kit |
48T |
reddotbiotech |
|
RD-FGF12-Mu |
Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit |
96T |
reddotbiotech |
|
RD-FGF12-Mu |
Mouse Fibroblast Growth Factor 12 (FGF12) ELISA Kit |
48T |
reddotbiotech |
|
RD-FGF12-Ra |
Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit |
96T |
reddotbiotech |
|
RD-FGF12-Ra |
Rat Fibroblast Growth Factor 12 (FGF12) ELISA Kit |
48T |
reddotbiotech |
上海金畔生物科技有限公司是实验试剂一站式采购服务商
1：强大的进口辐射能力，血清、抗体、耗材、大部分限制进口品等。
2：产品种类齐全，经营超过700多个品牌，基本涵盖所有生物实验试剂耗材。
3：提供加急服务，货品一般1-2周到货。
4：富有竞争力的价格优势，绝大部分价格有优势。
5：多年积累良好的信誉，大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校，ROCHE，阿斯利康、国药、fisher等药企。
6：我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司代理。
7：我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发，欢迎合作。
FGF-23 ELISA Kit Kainos 说明书
世界*实验材料供应商 Kainos Laboratories上海金畔生物为其中国代理， Kainos Laboratories在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， Kainos Laboratories就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
Kainos Laboratories中国代理, Kainos Laboratories上海代理， Kainos Laboratories北京代理，Kainos Laboratories广东代理， Kainos Laboratories江苏代理Kainos Laboratories湖北代理,Kainos Laboratories天津,Kainos Laboratories黑龙江代理,Kainos Laboratories内蒙古代理,Kainos Laboratories吉林代理,Kainos Laboratories福建代理, Kainos Laboratories江苏代理, Kainos Laboratories浙江代理, Kainos Laboratories四川代理,
Kainos Laboratories 是日本的生物公司，其生产的FGF-23 ELISA Kit和ADAMTS13-cleaved广泛应用于各大医药企业。Kainos产品包括各种生物化学临床检测试剂系统，如用于自动分析设备的“Aqua-auto Kainos”系列; “Lana 1,G”是一种高灵敏度糖尿病诊断试剂和一系列免疫血清学检测试剂;“MAST”，一种过敏性诊断试剂; “Hitazyme Chlamydia”，用于传染病。其他流感检测试剂盒“Statmark FLUstick-N”，用于A群β-溶血性链球菌的抗原检测试剂盒“Statmark Strep A”等。
货号：CY-4000
品名：FGF-23 ELISA Kit （FGF-23酶联免疫吸附试剂盒）
一、FGF-23 ELISA试剂盒的包装规格
|
CY-4000 |
品名 |
规格 |
数量 |
包装尺寸 |
总重 |
|---|---|---|---|---|---|
|
CY-4000 |
FGF-23 ELISA Kit |
96t |
1 kit |
28x37x29 cm |
6.0 kg |
|
CY-4000 |
FGF-23 ELISA Kit |
2 x 96t |
2 kits |
28x37x29 cm |
6.5 kg |
|
CY-4000 |
FGF-23 ELISA Kit |
3 x 96t |
3 kits |
35x42x30 cm |
7.5 kg |
|
CY-4000 |
FGF-23 ELISA Kit |
4 x 96t |
4 kits |
35x42x30 cm |
8.0 kg |
|
CY-4000 |
FGF-23 ELISA Kit |
5 x 96t |
5 kits |
35x42x30 cm |
9.0 kg |
|
CY-4000 |
FGF-23 ELISA Kit |
10 x 96t |
10 kits |
37x66x32 cm |
15.0 kg |
二、试剂盒组成
1） FGF-23抗体包被微孔板（FGF-23板）
用抗FGF-23的鼠单克隆抗体包被微孔板96孔微孔板。
2） HRP标记的FGF-23抗体（FGF-23缀合物）
12mL与辣根过氧化物酶缀合的FGF-23的鼠单克隆抗体与防腐剂混合。该试剂应储存在2-10℃并避光。
3） FGF-23校准液（STD1～std7）
用防腐剂缓冲FGF-23的FGF-23标准品（Std1-Std7）12mL（Std1）和0.5mL（Std2-Std7）。7瓶含有防腐剂的缓冲碱的FGF-23。
4） 测定稀释剂（Assay Diluent）
12 mL缓冲液。
5） 显色剂（底物）
12mL四甲基联苯胺TMBZ）与过氧化氢脲。
6） FGF-23清洗缓冲液（10倍浓缩）
53毫升缓冲表面活性剂与防腐剂的10倍浓缩溶液。使用前，用去离子水将内容物稀释至10倍并充分混合。
7） 终止液（终止液）
12 mL 0.5 mol / L硫酸。
8） 封板器
试剂盒中包含密封板三。
* 这些试剂应在黑暗中储存在2-10℃。使用前将所有试剂置于室温下。
三、试剂盒检测原理
FGF-23 ELISA试剂盒是用于测量血清中FGF-23的双位点酶联免疫吸附测定试剂盒。两种特异性小鼠单克隆抗体结合全长FGF-23。其中一种抗体固定在微量滴定板上进行捕获。另一种抗体与HRP（辣根过氧化物酶）缀合用于检测。
在*个反应中，将含有FGF-23的样品与固定在微量滴定孔的抗体一起培养。用抗体捕获样品中的FGF-23。在该反应结束时，洗涤该孔以去除未结合的FGF-23和其他组分。
在第二个反应中，将这种固定的FGF-23与HRP标记的抗体一起温育以形成“三明治”复合物：抗FGF-23抗体 – N端FGF-23C端-HRP标记的抗FGF-23抗体。在该反应结束时，洗涤该孔以除去未结合的组分。
在酶反应中，将固定在孔上的夹心复合物与底物溶液一起孵育，然后用分光光度微量滴定板读数器测量。与孔结合的复合物的酶活性与样品中FGF-23的量成正比。通过绘制吸光度与FGF-23标准品各浓度的关系绘制标准曲线。从该曲线确定样品中FGF-23的浓度。
四、产品使用注意事项
|
1） |
测试样品的储存条件是什么？ |
|
|
使用鲜的血清样本。如果您无法立即测试您的样品，请将其存放在-70°C以下。避免解冻/重新冷却循环。 在我们的实验室测试中，在冰箱中13天后，给定样品的活性降至86.6％。样品在-80°C下可保持稳定约三年，但在-20°C时稳定性不能得到保证。 我们已确认zui多六次解冻/再次冻结循环可能不会影响活动，但我们建议小批量冻结样本，以尽量缩短解冻/再次冻结周期。 |
|
2） |
试剂盒检测的样本 |
|
|
虽然血浆和血清都可以使用FGF-23 ELISA试剂盒进行检测，但建议使用血清。 |
|
3） |
试剂盒是否可以测试动物样本？ |
|
|
FGF-23ELISA试剂盒可用于测量小鼠，大鼠或猴血FGF-23水平。 |
|
4） |
试剂盒是否含有任何汞基防腐剂？ |
|
|
FGF-23 ELISA试剂盒不使用水银防腐剂，如硫柳汞。标准溶液，稀释溶液，酶标抗体溶液和浓缩洗涤溶液使用Kathon TM CG作为防腐剂。 |
|
5） |
操作预防措施 |
|
|
校准曲线必须为每次测试准备。 如果测试多个样品，则反应时间必须保持一致。 搅拌板时必须进行一级反应。搅拌前应密封盘子。 洗涤周期中必须遵守以下预防措施： 不要将洗版溶液留在其中。 一旦从井中充分移除洗涤液，立即进入下一步。 |
五、试剂使用方法
|
1） |
在支架上准备足够数量的抗体涂层带。 |
|
2） |
向孔中加入50μL检测稀释剂。 |
|
3） |
向每个孔中加入50μLFGF-23标准品或样品，并用平板密封器盖住平板。 |
|
4） |
在板式混合器上在室温下孵育平板2小时。 |
|
5） |
拆卸封板器。吸取每孔中的样品。用300μL工作洗涤缓冲液洗涤每个孔4次，并*吸出内容物。 |
|
6） |
向每个孔中加入100μL的FGF-23结合物并用封板器覆盖该板。 |
|
7) |
在平板混合器上在室温下孵育平板1小时。 |
|
8) |
拆卸封板器。吸取每孔中的样品。每孔用300μL工作洗涤缓冲液洗涤4次，并将其*吸出。 |
|
9) |
向每个孔中加入100μL底物，并用平板封口机和铝箔覆盖平板。 |
|
10) |
在室温下孵育板30分钟。 |
|
11) |
去除封口机和铝箔。立即加入100μL终止液到每个孔。在平板混合器上摇动板1分钟。 |
|
12) |
用微量滴定板读数器在10分钟内读取450nm处的吸光度。 |
|
准备好所有的试剂和标准品 |
|
|
|
|
|
向每个孔中加入50μL测定稀释液 |
|
|
|
|
|
每孔加入50μLFGF-23标准品或样品 |
|
|
|
|
|
在平板混合器上在室温下孵育2小时 |
|
|
|
|
|
抽吸并洗涤4次 |
|
|
|
|
|
每孔加入100μL的FGF-23共轭物 |
|
|
|
|
|
在平板混合器上在室温下孵育1小时 |
|
|
|
|
|
抽吸并洗涤4次 |
|
|
|
|
|
每孔加入100μL底物 |
|
|
|
|
|
在室温下孵育30分钟避光 |
|
|
|
|
|
在每孔中加入100μLStop Solution 在10分钟内在450nm处读数 |
|
六、性能表现
1）校准曲线
1）线性度
2）灵敏性
FGF-23 ELISA Kit，大量现货。
Mouse KGF-2/FGF-10 重组小鼠角质形成细胞生长因子-2/FGF-10(小鼠KGF-2/FGF-10)
Mouse KGF-2/FGF-10 重组小鼠角质形成细胞生长因子-2/FGF-10(小鼠KGF-2/FGF-10)
产品说明书
FAQ
COA
已发表文献
Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is a stromal-derived paracrine growth factor considered to be important during embryogenesis. FGF-10 plays an important role in adipogenesis, at least partly by contributing to the expression of C/EBPβ through an autocrine/paracrine mechanism. During cardiogenesis, FGF-10 is expressed in the anterior second heart field. Together with Fibroblast growth factor 8 (FGF-8), FGF10 promotes the proliferation of these cardiac progenitor cells that form the arterial pole of the heart.
Product Properties
|
Synonyms |
FGF10, FGF-10, fibroblast growth factor 10, Keratinocyte growth factor 2, KGF2, KGF-2 |
|
Accession |
O35565 |
|
GeneID |
14165 |
|
Source |
E.coli-derived mouse KGF-2/FGF-10, Gln37-Thr209. |
|
Molecular Weight |
Approximately 19.5 kDa. |
|
AA Sequence |
QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
>95% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
HB220214
Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is a stromal-derived paracrine growth factor considered to be important during embryogenesis. FGF-10 plays an important role in adipogenesis, at least partly by contributing to the expression of C/EBPβ through an autocrine/paracrine mechanism. During cardiogenesis, FGF-10 is expressed in the anterior second heart field. Together with Fibroblast growth factor 8 (FGF-8), FGF10 promotes the proliferation of these cardiac progenitor cells that form the arterial pole of the heart.
Product Properties
|
Synonyms |
FGF10, FGF-10, fibroblast growth factor 10, Keratinocyte growth factor 2, KGF2, KGF-2 |
|
Accession |
O35565 |
|
GeneID |
14165 |
|
Source |
E.coli-derived mouse KGF-2/FGF-10, Gln37-Thr209. |
|
Molecular Weight |
Approximately 19.5 kDa. |
|
AA Sequence |
QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
>95% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
HB220214
暂无内容
暂无内容
Human FGF-10 重组人成纤维细胞生长因子10/(人FGF-10)
Human FGF-10 重组人成纤维细胞生长因子10/(人FGF-10)
产品说明书
FAQ
COA
已发表文献
产品信息
|
产品名称 |
货号 |
规格 |
|
Recombinant Human KGF-2/FGF-10 Protein |
91306ES05 |
2 μg |
|
91306ES10 |
10 μg |
|
|
91306ES50 |
50 μg |
|
|
91306ES60 |
100 μg |
|
|
91306ES76 |
500 μg |
产品描述
成纤维细胞生长因子10（FGF-10）是一种肝素结合生长因子，属于FGF家族。该家族的蛋白质通过促进细胞增殖和分化，在各种组织的产前发育、产后生长和再生过程中发挥着核心作用。FGF-10与KGF/FGF-7最相关，在发育过程中表达，优先在成人肺中表达。它通过FGFR 2b发出信号。
重组人FGF-10是一种常见的类器官培养因子。本品是由大肠杆菌原核表达系统制备的重组蛋白，经分离纯化、过滤除菌和冻干等步骤制成，具有高纯度、高活性、无标签及低内毒素等特点。
性能参数
|
分子别名 |
FGF10, FGF-10, fibroblast growth factor 10, Keratinocyte growth factor 2, KGF2, KGF-2 |
|
表达系统及表达区间 |
Human FGF-10 is expressed from E.coli with no tag . It contains Leu40-Ser208.. |
|
Accession |
O15520 |
|
分子量 |
Approximately 19.1 kDa. |
|
生物活性 |
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
外观 |
无菌过滤,白色冻干粉末。 |
|
纯度 |
> 97%，SDA-PAGE 检测 |
|
内毒素 |
<1.0 EU/μg |
|
制剂 |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
|
复溶方法 |
建议在打开之前短暂离心，以将内容物带到底部。在无菌蒸馏水或者含有0.1% BSA的水性缓冲液中重新配制至0.1-1.0 mg/mL的浓度。储备溶液应分成等分试样，并在≤-20°C下储存。 |
储存条件
-25~-15℃保存，收到货之后有效期1年。
复溶后， 2 ~8 °C保存，30天有效期。复溶后， -25~-15℃保存，3个月有效期。
建议首次使用时将蛋白等分，避免重复冻融循环。
注意事项
1. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
相关产品
|
产品名称 |
货号 |
规格 |
|
Human noggin |
92528ES10 |
10μg |
|
Human R-Spondin 1 |
92278ES10 |
10μg |
|
Human EGF |
92701ES60 |
100μg |
|
Human Wnt-3a |
92278ES10 |
10μg |
|
Human FGF-2/bFGF |
91330ES10 |
10μg |
|
Human FGF-7/KGF-1 |
91304ES10 |
10μg |
|
Human BMP-4 |
92053ES20 |
20μg |
|
Human BMP-7 |
92054ES10 |
10μg |
|
Human HGF |
92055ES10 |
10μg |
|
Human Epiregulin |
92516ES08 |
5μg |
|
Human BMP-2 |
92051ES10 |
10μg |
产品信息
|
产品名称 |
货号 |
规格 |
|
Recombinant Human KGF-2/FGF-10 Protein |
91306ES05 |
2 μg |
|
91306ES10 |
10 μg |
|
|
91306ES50 |
50 μg |
|
|
91306ES60 |
100 μg |
|
|
91306ES76 |
500 μg |
产品描述
成纤维细胞生长因子10（FGF-10）是一种肝素结合生长因子，属于FGF家族。该家族的蛋白质通过促进细胞增殖和分化，在各种组织的产前发育、产后生长和再生过程中发挥着核心作用。FGF-10与KGF/FGF-7最相关，在发育过程中表达，优先在成人肺中表达。它通过FGFR 2b发出信号。
重组人FGF-10是一种常见的类器官培养因子。本品是由大肠杆菌原核表达系统制备的重组蛋白，经分离纯化、过滤除菌和冻干等步骤制成，具有高纯度、高活性、无标签及低内毒素等特点。
性能参数
|
分子别名 |
FGF10, FGF-10, fibroblast growth factor 10, Keratinocyte growth factor 2, KGF2, KGF-2 |
|
表达系统及表达区间 |
Human FGF-10 is expressed from E.coli with no tag . It contains Leu40-Ser208.. |
|
Accession |
O15520 |
|
分子量 |
Approximately 19.1 kDa. |
|
生物活性 |
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
外观 |
无菌过滤,白色冻干粉末。 |
|
纯度 |
> 97%，SDA-PAGE 检测 |
|
内毒素 |
<1.0 EU/μg |
|
制剂 |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
|
复溶方法 |
建议在打开之前短暂离心，以将内容物带到底部。在无菌蒸馏水或者含有0.1% BSA的水性缓冲液中重新配制至0.1-1.0 mg/mL的浓度。储备溶液应分成等分试样，并在≤-20°C下储存。 |
储存条件
-25~-15℃保存，收到货之后有效期1年。
复溶后， 2 ~8 °C保存，30天有效期。复溶后， -25~-15℃保存，3个月有效期。
建议首次使用时将蛋白等分，避免重复冻融循环。
注意事项
1. 本产品仅作科研用途。
2. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
相关产品
|
产品名称 |
货号 |
规格 |
|
Human noggin |
92528ES10 |
10μg |
|
Human R-Spondin 1 |
92278ES10 |
10μg |
|
Human EGF |
92701ES60 |
100μg |
|
Human Wnt-3a |
92278ES10 |
10μg |
|
Human FGF-2/bFGF |
91330ES10 |
10μg |
|
Human FGF-7/KGF-1 |
91304ES10 |
10μg |
|
Human BMP-4 |
92053ES20 |
20μg |
|
Human BMP-7 |
92054ES10 |
10μg |
|
Human HGF |
92055ES10 |
10μg |
|
Human Epiregulin |
92516ES08 |
5μg |
|
Human BMP-2 |
92051ES10 |
10μg |
暂无内容
暂无内容
Mouse FGF-9 重组小鼠成纤维细胞生长因子9(小鼠FGF-9)
Mouse FGF-9 重组小鼠成纤维细胞生长因子9(小鼠FGF-9)
产品说明书
FAQ
COA
已发表文献
Fibroblast growth factor-9, also called FGF-9, is an approximately 26 kDa secreted glycoprotein of the FGF family. Secreted human FGF-9 is a 205-207 aa protein that lacks the N-terminal 1-3 aa and shares 98% sequence identity with mouse, rat, equine, porcine and bovine FGF-9. In addition to FGF R3 (IIIb), FGF-9 binding to the IIIc splice forms of FGF R1, R2 and R3 are variably reported. FGF-9 is an autocrine/paracrine growth factor considered to be important for the growth and survival of motorneurons and prostate. Meanwhile, FGF9 is a potent mitogen and survival factor required for morphogenesis during embryonic development and numerous biological functions at adulthood. FGF9 have been shown to improve systolic function after myocardial infarction in a clinical trial. FGF9 promotes cardiac vascularization during embryonic development but is only weakly expressed in the adult heart.
Product Properties
|
Synonyms |
FGF9, FGF-9, Glia-activating factor, GAF, glia-activating factor, HBFG-9, HBGF-9 |
|
Accession |
P54130 |
|
GeneID |
14180 |
|
Source |
E.coli-derived mouse FGF-9, Pro3-Ser208, with an N-terminal Met. |
|
Molecular Weight |
Approximately 23.4 kDa. |
|
AA Sequence |
MPLGEVGSYF GVQDAVPFGN VPVLPVDSPV LLNDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
>95% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 8.5. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
HB220214
Fibroblast growth factor-9, also called FGF-9, is an approximately 26 kDa secreted glycoprotein of the FGF family. Secreted human FGF-9 is a 205-207 aa protein that lacks the N-terminal 1-3 aa and shares 98% sequence identity with mouse, rat, equine, porcine and bovine FGF-9. In addition to FGF R3 (IIIb), FGF-9 binding to the IIIc splice forms of FGF R1, R2 and R3 are variably reported. FGF-9 is an autocrine/paracrine growth factor considered to be important for the growth and survival of motorneurons and prostate. Meanwhile, FGF9 is a potent mitogen and survival factor required for morphogenesis during embryonic development and numerous biological functions at adulthood. FGF9 have been shown to improve systolic function after myocardial infarction in a clinical trial. FGF9 promotes cardiac vascularization during embryonic development but is only weakly expressed in the adult heart.
Product Properties
|
Synonyms |
FGF9, FGF-9, Glia-activating factor, GAF, glia-activating factor, HBFG-9, HBGF-9 |
|
Accession |
P54130 |
|
GeneID |
14180 |
|
Source |
E.coli-derived mouse FGF-9, Pro3-Ser208, with an N-terminal Met. |
|
Molecular Weight |
Approximately 23.4 kDa. |
|
AA Sequence |
MPLGEVGSYF GVQDAVPFGN VPVLPVDSPV LLNDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY KDILSQS |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
>95% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 8.5. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
HB220214
暂无内容
暂无内容
Human FGF-9 重组人成纤维细胞生长因子9(人FGF-9)
Human FGF-9 重组人成纤维细胞生长因子9(人FGF-9)
产品说明书
FAQ
COA
已发表文献
Human FGF-9 protein, also known as fibroblast growth factor 9, is a member of the fibroblast growth factor (FGF) family. It functions as a signaling molecule, regulating various cellular processes like cell proliferation, differentiation, and migration. FGF-9 protein plays a vital role in various physiological and pathological processes, making it an important focus of research in the field of molecular biology and medicine.
性能参数
|
Synonyms |
FGF9, FGF-9, Glia-activating factor, GAF, glia-activating factor, HBFG-9, HBGF-9 |
|
Uniprot No. |
P31371 |
|
Source |
E.coli-derived human FGF-9 protein, Leu4-Ser208, with C-terminal His tag. |
|
Molecular Weight |
Approximately25.2kDa. |
|
Purity |
> 90% as determined by SDS-PAGE. |
|
Endotoxin |
< 1 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Liquid in PBS，the concentration is 0.3 mg/ml. |
储存条件
The product should be stored at -25~–15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~–15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves, for your safety.
2.This product is for research use only.
产品数据
Ver. CN20230628
Human FGF-9 protein, also known as fibroblast growth factor 9, is a member of the fibroblast growth factor (FGF) family. It functions as a signaling molecule, regulating various cellular processes like cell proliferation, differentiation, and migration. FGF-9 protein plays a vital role in various physiological and pathological processes, making it an important focus of research in the field of molecular biology and medicine.
性能参数
|
Synonyms |
FGF9, FGF-9, Glia-activating factor, GAF, glia-activating factor, HBFG-9, HBGF-9 |
|
Uniprot No. |
P31371 |
|
Source |
E.coli-derived human FGF-9 protein, Leu4-Ser208, with C-terminal His tag. |
|
Molecular Weight |
Approximately25.2kDa. |
|
Purity |
> 90% as determined by SDS-PAGE. |
|
Endotoxin |
< 1 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Liquid in PBS，the concentration is 0.3 mg/ml. |
储存条件
The product should be stored at -25~–15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~–15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves, for your safety.
2.This product is for research use only.
产品数据
Ver. CN20230628
暂无内容
暂无内容
Kainos FGF-23 ELISA Kit说明书
Kainos Laboratories 是日本的生物公司，其生产的FGF-23 ELISA Kit和ADAMTS13-cleaved广泛应用于各大医药企业，上海金畔生物科技有限公司为您带来Kainos Laboratories高品质的产品，查询 http://www.kainos.co.jp
Kainos Laboratories代理 Kainos Laboratories中国代理，Kainos Laboratories上海代理，Kainos Laboratories代理，Kainos Laboratories北京代理
| This kit captures ADAMTS13-cleaved products using a sandwich method with the anti-GST mouse monoclonal antibody and the peroxidase-conjugated mouse monoclonal anti-N10 antibody. First, the anti-GST mouse monoclonal antibody immobilized on to the microplate reacts with the VWF73 substrate（GST-VWF73-His）. The sample is then applied to the microplate, on which ADAMTS13 cleaves the VWF73 substrate. By applying the mouse monoclonal anti-N10 antibody conjugated with horseradish peroxidase（HRP conjugated antibody）, the cleavage product is sandwiched between the two antibodies. Because the amount of the cleavage products depends on the ADAMTS13 activity, the amount of the enzyme-labeled antibodies that bind to the cleavage products also reflect the level of ADAMTS13 activity. Therefore, the plasma ADAMTS13 activity can be determined by colorimetrically measuring the amount of detached oxidized （colored）TMBZ using urea hydrogen peroxide（H2O2）and 3,3’,5,5’-Tetramethyl- benzidine（TMBZ）as the substrates. | ||
上海金畔生物科技有限公司
实验试剂一站式采购服务商
1：强大的进口辐射能力，血清、抗体、耗材、大部分限制进口品等。
2：产品种类齐全，经营超过700多个品牌，基本涵盖所有生物实验试剂耗材。
3：提供加急服务，货品一般1-2周到货。
4：富有竞争力的价格优势，绝大部分价格有优势。
5：多年积累良好的信誉，大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校，ROCHE，阿斯利康、国药、fisher等药企。
6：我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司代理。
7：我们还是invitrogen,qiagen,Tempshield, Inc.am,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发，欢迎合作。