标签归档：fc

athensresearch 16-16-090707-1M-FC

发表于由

athensresearch 16-16-090707-1M-FC说明书

Immunoglobulin G1 (IgG1), Human Myeloma Plasma, FC Fragment

免疫球蛋白G1（IgG1），人骨髓瘤血浆，FC片段
尺寸许可数量
1毫克$230.00

产品编号：16-16-090707-1M-FC
打印页面电子邮件页面链接
规格
直接从制造商处购买。
批量可用。
分子量：50000 Da，通过SDS-PAGE
消光系数：1.35
在50 mM Tris HCl中冷冻，pH 8.0，200 mM NaCl和0.05%NaN3。
由两个免疫球蛋白重链的羧基末端通过二硫键相互连接而成的可结晶片段。Fc片段包含负责免疫球蛋白效应器功能的重链恒定区的羧基末端部分（补体固定、通过Fc受体与细胞膜结合和胎盘转运）。IgG1 FC作为一种潜在的抗炎药在治疗人类自身免疫性疾病中有着新的作用。
Anthony R，Ravetch J等人。（2010），临床免疫学杂志。30补遗1:S9-14
IgG1-FC片段不与抗原结合，但在细胞营养反应、补体结合和分解代谢率控制方面，它包含经典的抗原决定簇和生物活性。
储存温度：-20°C
纯度：SDS-PAGE法大于等于95%。
由经FDA批准的HBsAg、抗HCV、抗HBc和抗HIV 1&2阴性的血浆制备。
雅典研究与技术产品是实验室试剂，不得用于人类或任何药物用途。仅用于研究或进一步制造。

 

 

 

 

16-16-090707-LC

Immunoglobulin G (IgG), Light Chain, Human Plasma

16-16-090707

Immunoglobulin G (IgG), Normal Human Plasma

16-16-090707-dog

Immunoglobulin G, Dog Plasma

16-16-090707-FAB2

Immunoglobulin G, Fab '2, Human Plasma

16-16-090707-Fab

Immunoglobulin G, Fab Fragment, Human Plasma

16-16-090707-FC

Immunoglobulin G, FC Fragment, Human Plasma

16-16-090707-FC-IMM

Immunoglobulin G, FC Fragment, Human Plasma, Immunogen Grade

16-16-090707-FC-LEL

Immunoglobulin G, FC Fragment, Low Endotoxin Level, Human Plasma

16-16-090707-mouse

Immunoglobulin G, Mouse Plasma

16-16-090707-RAT

Immunoglobulin G, Rat Serum

16-16-090707-1M

Immunoglobulin G1 (IgG1), Human Myeloma Plasma

16-16-090707-1M-FAB

Immunoglobulin G1 (IgG1), Human Myeloma Plasma, Fab Fragment

16-16-090707-1M-FC

Immunoglobulin G1 (IgG1), Human Myeloma Plasma, FC Fragment

16-16-090707-1

Immunoglobulin G1 (IgG1), Normal Human Plasma

16-16-090707-2M

Immunoglobulin G2 (IgG2), Human Myeloma Plasma

16-16-090707-2

Immunoglobulin G2 (IgG2), Normal Human Plasma

16-16-090707-3

Immunoglobulin G3 (IgG3), Normal Human Plasma

16-16-090707-4M

Immunoglobulin G4 (IgG4), Human Myeloma Plasma

16-16-090707-4

Immunoglobulin G4 (IgG4), Normal Human Plasma

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

发表于由

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

产品说明书

FAQ

COA

已发表文献

 

Fc gamma RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta. Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA. Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity. Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis. Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases.

 

产品性质

别名

Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20

Accession

AAH17865

表达区间及表达系统

Recombinant Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Gln208.

分子量

Approximately 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

ELISA Data：Immobilized Human Fc gamma RIIIA (V176) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 0.09μg/ml determined by ELISA.

SPR Data：Human Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200).

内毒素

< 1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

 

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白
重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

 

HB220811

 

 

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

暂无内容

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

暂无内容

 

Fc gamma RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta. Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA. Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity. Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis. Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases.

 

产品性质

别名

Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20

Accession

AAH17865

表达区间及表达系统

Recombinant Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Gln208.

分子量

Approximately 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

ELISA Data：Immobilized Human Fc gamma RIIIA (V176) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 0.09μg/ml determined by ELISA.

SPR Data：Human Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200).

内毒素

< 1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

 

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白
重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

 

HB220811

 

 

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

暂无内容

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

暂无内容

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

发表于由

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

产品说明书

FAQ

COA

已发表文献

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. Glycosylation of the EPO protein is required for biological activities in vivo . The mature human EPO protein shares 75% – 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells . Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations.

 

Product Properties

Synonyms

Erythropoietin, Epoetin

Accession

P01588

Gene ID

2056

Source

HEK293 Cells-derived human EPO protein, Met1-Arg193 with hFc Tag at the C-terminus

Molecular Weight

Approximately 45.1 kDa.

AA Sequence

MGVHECPAWL WLLLSLLSLP LGLPVLGAPP RLICDSRVLE RYLLEAKEAE NITTGCAEHC SLNENITVPDTKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQALLVN SSQPWEPLQL HVDKAVSGLR SLTTLLRALG AQKEAISPPD AASAAPLRTI TADTFRKLFR VYSNFLRGKL KLYTGEACRT GDR

Tag

hFc Tag

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE.

Biological Activity

1. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2.0-8.0 ng/mL.
2. Measured by its ability to bind Human EPOR (ECD, His Tag) in functional ELISA.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only.

 

 

HB220415

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

暂无内容

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

暂无内容

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. Glycosylation of the EPO protein is required for biological activities in vivo . The mature human EPO protein shares 75% – 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells . Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations.

 

Product Properties

Synonyms

Erythropoietin, Epoetin

Accession

P01588

Gene ID

2056

Source

HEK293 Cells-derived human EPO protein, Met1-Arg193 with hFc Tag at the C-terminus

Molecular Weight

Approximately 45.1 kDa.

AA Sequence

MGVHECPAWL WLLLSLLSLP LGLPVLGAPP RLICDSRVLE RYLLEAKEAE NITTGCAEHC SLNENITVPDTKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQALLVN SSQPWEPLQL HVDKAVSGLR SLTTLLRALG AQKEAISPPD AASAAPLRTI TADTFRKLFR VYSNFLRGKL KLYTGEACRT GDR

Tag

hFc Tag

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE.

Biological Activity

1. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2.0-8.0 ng/mL.
2. Measured by its ability to bind Human EPOR (ECD, His Tag) in functional ELISA.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only.

 

 

HB220415

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

暂无内容

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

暂无内容

affinitybiologicals F8C-EIA现货

发表于由

affinitybiologicals F8C-EIA现货

Factor VIII Paired Antibody Set

Affinity的因子VIII配对抗体集由匹配的捕获和检测抗体组成，这些抗体已被滴定并优化用于三明治式ELISA测定。所提供的产品包含足够的捕获和检测抗体，用于四个完整的96孔微孔板，并包含详细的方案表，其中包含使用说明、溶液配方和所需额外材料的来源。该因子VIII配对抗体集旨在促进最终用户建立仅用于研究目的的“内部”免疫测定，不得用于诊断应用。化验验证由最终用户负责。

材料：

捕获抗体（F8C-EIA-C）：一个黄色盖小瓶，含有0.4mL多克隆亲和纯化的抗因子VIII抗体，用于包衣板

检测抗体（F8C-EIA-D）：四个较大的管，每个管含有10.0 mL过氧化物酶偶联的亲和纯化的多克隆抗因子VIII抗体，用于检测捕获的因子FVIII

样品稀释液（F8C-EIA-SD）：100.0 mL瓶，含有针对样品稀释而优化的绿色稀释液

Cat # Format
F8C-EIA

1 x 0.4 mL Capture Antibody (F8C-EIA-C)

4 x 10.0 mL Detecting Antibody (F8C-EIA-D)

1 x 100.0 mL Sample Diluent (F8C-EIA-SD)

Innovex Fc受体阻断剂说明书

发表于由

 Innovex Fc受体阻断剂说明书

Innovex Fc受体阻断剂 (Innovex Fc Receptor Block) | 货号NB309

 

Fc受体（ 受体（Fc receptors , FcR Fc receptors , FcR）是分⼦量约为 ）是分⼦量约为50-70 kD 50-70 kD的糖蛋⽩ 的糖蛋⽩(glycoproteins) (glycoproteins)。

他们广泛在整个免疫细胞 。他们广泛在整个免疫细胞(immune (immune cells) cells)中表达。

它们存在于⽩⾎球 中表达。它们存在于⽩⾎球(leukocytes) (leukocytes)如单核细胞 如单核细胞(monocytes) (monocytes)，组织巨噬细胞 ，组织巨噬细胞(tissue macrophages) (tissue macrophages)，，BB细胞细胞 (B cells) (B cells)，粒细胞 ，粒细胞(granulocytes) (granulocytes)嗜酸性粒细胞 嗜酸性粒细胞(eosinophils) (eosinophils)，嗜碱性粒细胞 ，嗜碱性粒细胞(basophils) (basophils)，嗜中性粒细胞 ，嗜中性粒细胞 (neutrophils) (neutrophils)，，NKNK细胞和⼀些 细胞和⼀些TT细胞。 细胞。Fc receptors Fc receptors肥⼤细胞，滤泡树突细胞 肥⼤细胞，滤泡树突细胞(follicular dendritic cells) (follicular dendritic cells)，上⽪细胞 ，上⽪细胞 (epithelial cells) (epithelial cells)，内⽪细胞 ，内⽪细胞(endothelial cells) (endothelial cells)，肝细胞上也存在受体和 ，肝细胞上也存在受体和langerhans cells langerhans cells等等。

 Fc Fc受体（ 受体（FcR FcR）有几 ）有几 种类型，它们基于他们所识别的抗体上。有⼀些对单体 种类型，它们基于他们所识别的抗体上。有⼀些对单体IgGIgG的的FcFc区具有很⾼的亲和⼒抗体，称为 区具有很⾼的亲和⼒抗体，称为Fc-Gamma Fc-Gamma受体，与 受体，与 IgA IgA抗体结合的抗体称为 抗体结合的抗体称为Fc- Fc-αα受体，那些结合 受体，那些结合IgE IgE抗体的抗体称为 抗体的抗体称为Fc- Fc-εε受体。非特异性 受体。非特异性FcFc受体在 受体在IHC IHC，免疫荧光（ ，免疫荧光（IFIF）和）和 流式细胞仪等检测中可以染⾊。这是由于⼀抗和⼆抗的 流式细胞仪等检测中可以染⾊。这是由于⼀抗和⼆抗的FcFc区与细胞上 区与细胞上FcFc受体的结合所致。在 受体的结合所致。在IHC IHC，，IFIF和流式细胞仪 和流式细胞仪 中，消除Fc受体染⾊是理想的。 Innovex Fc Innovex Fc受体阻滞剂 受体阻滞剂 (Innovex Fc Receptor Blocker, NB309) (Innovex Fc Receptor Blocker, NB309) 是⼀种通⽤的 是⼀种通⽤的FcFc阻滞剂，适⽤于阻断所有类型的 阻滞剂，适⽤于阻断所有类型的FcFc Receptor Receptor受体，例如 受体，例如 I receptors I receptors ，，II receptors II receptors 和和III III型型Fc- Fc-γγ受体； 受体； Fc- Fc-εε受体受体II和和IIII型；型； Fc – Fc –αα受体， 受体，FcFcαα/ µR / µR和和FcRn FcRn。。 Innovex Fc阻断剂不包含抗体，免疫球蛋⽩或免疫球蛋⽩片段。 INNOVEX Fc阻断剂可⽤于阻断⼈类，⼩鼠和所有动 物等所有物种中的所有类型的 物等所有物种中的所有类型的FcFc受体， 受体， 通过多种免疫测定（例如 通过多种免疫测定（例如IHC IHC，免疫荧光（ ，免疫荧光（Immunouorescence, IF Immunouorescence, IF）和流式 ）和流式 细胞仪(Flow cytometry) ）检测细胞和组织。INNOVEX Fc Blocker is also commonly used in eliminating background staining in Brain cells/ tissues. Fc Receptor blocker is also used for obtaining specic staining for tissues stained for kappa, lambda antibodies and Immunoglobulins (lgs) by IHC, IF and Flow cytometry assays. INNOVEX Fc INNOVEX Fc阻断剂也常⽤于消除脑细胞 阻断剂也常⽤于消除脑细胞( Brain cells)/ ( Brain cells)/组织中 组织中(tissues) (tissues)的背景染⾊ Fc Fc受体阻滞剂也可⽤ 受体阻滞剂也可⽤kappa kappa ，， lambda antibodies lambda antibodies 及免疫球蛋⽩ 及免疫球蛋⽩( Immunoglobulins , lgs) ( Immunoglobulins , lgs)于于 IHC IHC，，IFIF和流式细胞进⾏特异性染⾊。 和流式细胞进⾏特异性染⾊。 如如CDCD表型表型(CD (CD phenotyping) phenotyping)，⽩⾎病分型 ，⽩⾎病分型(leukemia typing ) (leukemia typing )和活细胞功能测定 和活细胞功能测定( live cell functional assays) ( live cell functional assays)。。

 

产品介绍: Fc : Fc受体阻滞剂 受体阻滞剂 (Innovex Fc Receptor Block) (Innovex Fc Receptor Block) ，先进和*的功能；阻断⼈和动物细胞中的 ，先进和*的功能；阻断⼈和动物细胞中的FcFc受体。是准确 受体。是准确 的淋巴瘤，⽩⾎病和⿊⾊素瘤分型以及无背景 的淋巴瘤，⽩⾎病和⿊⾊素瘤分型以及无背景Kappa Kappa和和λλ染⾊的必需品。 染⾊的必需品。Innovex Fc Receptor Block Innovex Fc Receptor Block是⼀种基于 是⼀种基于 peptide based的技术，不含抗体，⾎清，免疫球蛋⽩，免疫球蛋⽩片段或链霉蛋⽩酶。⽬的为阻断所有⽩⾎球细胞 （（white blood cells white blood cells），淋巴瘤 ），淋巴瘤(lymphomas) (lymphomas)和⿊⾊素瘤 和⿊⾊素瘤(melanomas) (melanomas)中存在的 中存在的FcFc受体受体(Fc Receptors) (Fc Receptors)。在室温下对 。在室温下对 ⼈体组织 ⼈体组织//细胞进⾏ 细胞进⾏3030分钟的孵育步骤；动物组织 分钟的孵育步骤；动物组织//细胞孵育 细胞孵育4545分钟⾄ 分钟⾄11⼩时。消除⽩⾎球细胞，淋巴组织，细胞株 ⼩时。消除⽩⾎球细胞，淋巴组织，细胞株 （⾎液和骨髓），⿊⾊素瘤和其他表达Fc的肿瘤的假阳性染⾊，确保准确的淋巴瘤和⽩⾎病分型。 FcFc受体受体(Fc Receptors) (Fc Receptors)也在⼤多数肿瘤 也在⼤多数肿瘤(tumors) (tumors)上表达。阻断 上表达。阻断FcFc受体对于淋巴 受体对于淋巴(lymphoid) (lymphoid)和肿瘤组织 和肿瘤组织(tumor tissues) (tumor tissues) 和细胞的准确分型⾄关重要。 和细胞的准确分型⾄关重要。 Fc Fc受体染⾊通过细胞上存在的 受体染⾊通过细胞上存在的FcFc受体与初级和 受体与初级和//或第⼆抗体的 或第⼆抗体的FcFc区的结合⽽发⽣。 区的结合⽽发⽣。 Fc Fc受受体阻断剂可⽤于IHC IHC（冷冻和⽯蜡），免疫荧光 （冷冻和⽯蜡），免疫荧光(immunouorescence) (immunouorescence)，流式细胞术 ，流式细胞术(ow cytometry) (ow cytometry)和原位杂交 和原位杂交(in- (insitu hybridization) situ hybridization)。对于活细胞和功能测定，建议使⽤ 。对于活细胞和功能测定，建议使⽤Azide-Free Fc Block ( Azide-Free Fc Block (货号货号NB335) NB335)。。 产品特点:

1. Blocks Fc Receptors in both human and animal cells

2. 2. 30 minute incubation step at room temperature for human tissues/cells

 

  • 45 minutes to 1-hour incubation for animal tissues/cells
  • Eliminates false positive staining of white blood cells, lymphoid tissues, cytosmears (blood & bone marrow), melanoma tumors and other Fc expressing tumors
  • Insures accurate lymphoma and leukemia typing
  • liminates negative control staining of lymphomas, lymphoid tissues, and blood and bone marrow smears
  • Eliminates false positive staining for Kappa and Lambda staining
  • Eliminates false positive staining of Reed Sternberg cells
  • A must for CD markers staining in IHC, immunouorescence
  • A must for CD phenotyping via ow cytometry
  • A must for Immunoglobulins (Igs) staining via IHC, ow cytometry and immunouorescence

12. A must for kappa and lambda staining

 

absoluteantibody兽用 IgE Fc 蛋白简介

发表于由

absoluteantibody兽用 IgE Fc 蛋白简介

兽用 IgE Fc 蛋白

IgE 是一种经常被忽视的抗体同种型，但它在过敏反应和某些自身免疫性疾病中起着关键作用。为推进相关研究和诊断，Absolute Antibody 使用重组抗体技术在广泛的物种中开发高度可重复的 IgE 蛋白。这些 Fc-only 蛋白质可用于多种应用：

  • 检测标准：通过将 IgE 添加到正常血清中来创建可重现的对照血清

  • 免疫原：产生针对免疫球蛋白 Fc 域的抗体

  • 设计成抗原特异性抗体：为您的过敏或自身免疫研究制作 IgE 抗体

 

新物种可用

我们现在提供多种物种的 IgE Fc 结构域蛋白。我们从常见的模型、人和老鼠开始，最近扩大了我们的目录，包括狗、猫和马等动物。新物种是兽医研究和诊断分析设计的理想选择。

IgE Fc 结构域蛋白与物种特异性抗 IgE 抗体反应，未观察到交叉反应（参见下面的 ELISA 数据）。

目前可用的 IgE Fc 结构域蛋白如下所列。我们还提供其他亚型的免疫球蛋白 Fc 结构域，包括 Ig1、IgG2A、IgA 和 IgM；

目录编号 产品名称 介绍价格
Pr00104-14.5 重组人
IgE-Fc 域		 0.2mg 285 英镑 / 335 欧元 / 370 美元
Pr00107-20.5 重组小鼠
IgE-Fc 域		 0.2mg 285 英镑 / 335 欧元 / 370 美元
Pr00344-39.5 重组狗
IgE-Fc 域		 0.2mg 285 英镑 / 335 欧元 / 370 美元
Pr00343-38.5 重组猫
IgE-Fc 域		 0.2mg 285 英镑 / 335 欧元 / 370 美元
Pr00345-40.5 重组马
IgE-Fc 域		 0.2mg 285 英镑 / 335 欧元 / 370 美元