标签归档：fc

正常人骨髓间充质干细胞 FC-0057

发表于由

 

 

细胞

正常人类细胞和动物细胞

Lifeline®人类细胞包括：内皮细胞，上皮细胞，成纤维细胞，造血细胞，黑素细胞，平滑肌和间充质干细胞。所有Lifeline®正常人和动物（小鼠）细胞：

  • 当在生命线培养基中培养时，不需要暴露于抗微生物剂或酚红。不需要抗微生物剂和酚红，并且不推荐使用，以获得适的电池性能。这些补充剂可能导致细胞应激和“掩蔽效应”，可能会对实验结果产生负面影响。
  • 支原体呈阴性，细菌和真菌生长呈阴性。
  • 通过PCR对HIV，HBV和HCB呈阴性。

原代细胞定义为已从组织中分离，铺在培养容器上，扩增，收获和冷冻保存的细胞。

二级细胞定义为在收获用于冷冻保存之前已在培养容器中分离，铺板和扩增两次的细胞。

 

人类干细胞

间充质干细胞包括：脂肪来源的成体，wharton的果冻，骨髓和前脂肪细胞。

Lifeline®人类间充质干细胞产品为多能干细胞生物学和分化过程的研究提供了理想的模型。这些细胞可以在典型的间充质谱系中分化，例如脂肪形成，软骨形成和成骨。

Lifeline®开发了优化的培养基，以扩展未分化状态的间充质干细胞产品，以及用于诱导脂肪生成，软骨形成和成骨的优化培养基试剂盒。此外，Lifeline®提供方便的染色试剂盒，用于染色*分化的细胞。

 

正常人骨髓间充质干细胞  FC-0057 

Normal Human Bone Marrow Mesenchymal Stem Cells

 

产品描述

生命线®人骨髓间质干细胞（HMSC-BM）为多潜能干细胞生物学和分化过程的研究的理想培养模型。

从骨髓抽吸物中分离HMSC-BM，扩增一次，并作为二次细胞冷冻保存，以确保宜表型和gao活力和平板效率。

骨髓间充质干细胞可以在未分化状态下扩增，以便将来分化成多个谱系。

  • Lifeline®人间充质干细胞 – 骨髓可以在典型的间充质谱系中分化，例如脂肪形成，软骨形成和成骨。

通过流式细胞术表征人间充质干细胞 – 骨髓，以确保间充质干细胞的多种标志物的正确表达。它们对CD29，CD44，CD73，CD90，CD105和CD166均为阳性。它们对CD14，CD31，CD34和CD45均为阴性。

生命线®已经开发优化的媒体，扩大骨髓间充质干细胞产品在未分化的状态，以及优化的媒体套件诱导脂肪形成，软骨，和成骨。此外，生命线®提供染色*分化的细胞方便染色试剂盒。

 

每瓶100万个细胞

间充质干细胞-Bone Marrow的质量测试：

  • 形态学：3次传代的正常形态学
  • 细胞活力：从冷冻保存中解冻时，活力至少达70％
  • 无菌检测：支原体阴性。对细菌和真菌生长有负面影响
  • 病毒检测：PCR检测HIV-1，HIV-2，HBV和HCV阴性
  • 特异性染色：CD29，CD44，CD73，CD90，CD105，CD166的阳性*。CD14，CD31，CD34，CD45为
    负*。

* Lifeline®定义阳性表达，因为当超过95％的细胞群表达该细胞标记时。

* Lifeline®定义阴性表达，因为少于2％的细胞群表达该细胞标记。

 

 产品规格表

  •  

干细胞系统，人类

    • 人间充质干细胞（HMSC）系统和培养基 
    • 人间充质干细胞 – 骨髓

辅助产品

    • StemLife MSC-BM骨髓中等完整试剂盒 [LL-0062]
    • AdipoLife™DfKt™-2脂肪生成培养基试剂盒 [LL-0059]
    • ChondroLife™完整软骨形成培养基 [LM-0022]
    • OsteoLife™*成骨培养基 [LM-0023]

SDS和“安全数据表”

（MSDS或材料安全数据表）

  •  

货号

品名

规格

品牌

FC-0057

Normal Human Bone Marrow Mesenchymal Stem Cells

ea

lifelinecelltech

FC-0020

Normal Human Mesenchymal Stem Cells — Wharton’s Jelly

ea

lifelinecelltech

FC-0034

Human Mesenchymal Stem Cells — Adult

ea

lifelinecelltech

FC-0062

Normal Human Pre-Adipocyte Cells

ea

lifelinecelltech

HC-0023

Mouse Dendritic Cells, Primary

ea

lifelinecelltech

FC-0091

Human Skeletal Muscle Satellite Cells

ea

lifelinecelltech

FC-0014

Normal Human Aortic Endothelial Cells

ea

lifelinecelltech

FC-0032

Human Coronary Artery Endothelial Cells

ea

lifelinecelltech

FC-0058

Human Lung Microvascular Endothelial Cells

ea

lifelinecelltech

FC-0039

Dermal Microvascular Endothelial Cells – Adult

ea

lifelinecelltech

FC-0053

Cardiac Microvascular Endothelial Cells

ea

lifelinecelltech

FC-0042

Dermal Microvascular Endothelial Cells — Neonatal

ea

lifelinecelltech

FC-0055  

Normal Human Pulmonary Artery Endothelial Cells

ea

lifelinecelltech

FC-0003

Normal Human Umbilical Vein Endothelial Cells (HUVEC), Primary

ea

lifelinecelltech

FC-0044

Human Umbilical Vein Endothelial Cells (HUVEC) –10-Donor Pool

ea

lifelinecelltech

FC-0081

Fallopian Tube Epithelial Cells

ea

lifelinecelltech

FC-0083

Human Vaginal Epithelial Cells

ea

lifelinecelltech

FC-0078

Endometrial (Uterine) Epithelial Cells

ea

lifelinecelltech

FC-0080

Human Cervical Epithelial Cells

ea

lifelinecelltech

FC-0016

Normal Human Small Airway Epithelial Cells, Primary

ea

lifelinecelltech

FC-0035

Human Bronchial/Tracheal Epithelial Cells, Primary

ea

lifelinecelltech

FC-0054

Human Lobar Bronchial Epithelial Cells, Primary

ea

lifelinecelltech

FC-0079

Human Bladder Dome Epithelial Cells

ea

lifelinecelltech

FC-0040

Human Bladder Apex Epithelial Cells

ea

lifelinecelltech

FC-0029

Normal Human Corneal Epithelial Cells

ea

lifelinecelltech

FC-0094

Human Oral Keratinocytes (Gingiva)

ea

lifelinecelltech

FC-0007

Epidermal Keratinocytes — Neonatal, Primary

ea

lifelinecelltech

FC-0025

Epidermal Keratinocytes — Adult, Primary

ea

lifelinecelltech

FC-0064

Human Epidermal Keratinocytes, 10-Donor Pool

ea

lifelinecelltech

FC-0063

Normal Human Mammary Epithelial Cells — Male

ea

lifelinecelltech

FC-0065

Normal Human Mammary Epithelial Cells — Female

ea

lifelinecelltech

FC-0038

Normal Human Prostate Epithelial Cells

ea

lifelinecelltech

FC-0018

Human Renal Medullary Epithelial Cells, Primary

ea

lifelinecelltech

FC-0013

Renal Proximal Tubule Epithelial Cells

ea

lifelinecelltech

FC-0012

Human Renal Cortical Epithelial Cells, Primary

ea

lifelinecelltech

FC-0017

Normal Human Renal Mixed Epithelial Cells, Primary

ea

lifelinecelltech

FC-0048

Human Seminal Vesicle Epithelial Cells

ea

lifelinecelltech

FC-0060

Normal Human Cardiac Fibroblasts

ea

lifelinecelltech

FC-0095

Human Gingival Fibroblasts

ea

lifelinecelltech

FC-0050

Normal Human Bladder Fibroblasts

ea

lifelinecelltech

FC-0037

Human Dermal Fibroblasts – Neonatal, Xeno-Free, Primary

ea

lifelinecelltech

FC-0001

Human Dermal Fibroblasts – Neonatal, Primary

ea

lifelinecelltech

FC-0024

Normal Human Dermal Fibroblasts — Adult, Primary

ea

lifelinecelltech

FC-0049

Normal Human Lung Fibroblasts, Primary

ea

lifelinecelltech

FC-0076

Normal Human Uterine Fibroblasts

ea

lifelinecelltech

FC-0052

Normal Human Vas Deferens Fibroblasts

ea

lifelinecelltech

 

我们公司优势是强大的采购，

1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，

2：货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，

3：提供加急服务，一般1-2周到货，超过时限加急费全免

4：价格公道，绝大部分价格有优势，当然不能保证100%产品都是价格低廉,因为价格低廉意味着没有服务.

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重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

发表于由

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

产品说明书

FAQ

COA

已发表文献

 

Fc gamma RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta. Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA. Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity. Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis. Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases.

 

产品性质

别名

Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20

Accession

AAH17865

表达区间及表达系统

Recombinant Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Gln208.

分子量

Approximately 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

ELISA Data：Immobilized Human Fc gamma RIIIA (V176) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 0.09μg/ml determined by ELISA.

SPR Data：Human Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200).

内毒素

< 1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

 

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白
重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

 

HB220811

 

 

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

暂无内容

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

暂无内容

 

Fc gamma RIIIA/CD16a is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes. In humans, a single nucleotide polymorphism (T230A) creates high binding (176V) and low binding (176F) forms that may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies. Fc gamma RIIIA surface expression requires interaction with an accessory chain, either the common gamma -chain or CD3 zeta. Glycosylation patterns, electrophoretic mobility, and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIA. Shed forms of both Fc gamma RIIIA and Fc gamma RIIIB can be generated by proteolytic cleavage and retain binding activity. Shedding from monocytes and macrophages can be triggered by cell activation or phagocytosis. Soluble Fc gamma RIII circulates in normal plasma and is elevated in rheumatoid arthritis and in coronary artery diseases.

 

产品性质

别名

Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20

Accession

AAH17865

表达区间及表达系统

Recombinant Human Fc gamma RIIIA/CD16a (V176) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Gln208.

分子量

Approximately 24.7 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

ELISA Data：Immobilized Human Fc gamma RIIIA (V176) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Fc gamma RIIIA Antibody, hFc Tag with the EC50 of 0.09μg/ml determined by ELISA.

SPR Data：Human Fc gamma RIIIA (V176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 67.3nM as determined in SPR assay (Biacore T200).

内毒素

< 1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

 

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白
重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

 

HB220811

 

 

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

暂无内容

重组人Fc γ RIIIA/CD16a(V176)(His-Avi标签)免疫蛋白

暂无内容

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

发表于由

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统（Source）

Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-terminal. It contains Val26-Gln205.[uniprot  | P12319-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per ug by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human Fc epsilon RI alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody , hFc Tag with the EC50 of 14.1ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.    

 

Ver. CN20230525

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

暂无内容

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

暂无内容

性能参数

表达区间及表达系统（Source）

Recombinant Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with His tag at the C-terminal. It contains Val26-Gln205.[uniprot  | P12319-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 22.1 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per ug by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human Fc epsilon RI alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Fc epsilon RI alpha Antibody , hFc Tag with the EC50 of 14.1ng/ml determined by ELISA.

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.    

 

Ver. CN20230525

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

暂无内容

重组人FcεRIα/FCER1a蛋白(His标签) Human Fc epsilon RI alpha/FCER1a Protein

暂无内容

​formumax F70101C-AC说明书

发表于由

formumax F70101C-AC说明书

Combo: Clophosome®-A and Control Liposomes (anionic)

  • SKU:

  •  

  • F70101C-AC

  

组合：氯膦®-A和对照脂质体

该方便组合试剂盒包括1瓶阴离子氯膦酸盐脂质体和1瓶阴离子对照脂质体。你的储蓄超过20%。

产品代码：F7001C-AC

该套件由以下项目组成

1小瓶（2或10mL）氯膦酸盐®-A-阴离子氯膦酸盐脂质体（F7001C-A）

1小瓶（2或10mL）对照阴离子脂质体（F7001-A）

储存在冰箱中（2-8摄氏度）

重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

发表于由

重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统（Source）

Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Val26-Gln205.[Accession | P12319-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 10ng/ml determined by ELISA. 

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

Ver. CN20230625

重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

暂无内容

重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

暂无内容

性能参数

表达区间及表达系统（Source）

Human Fc epsilon RI alpha/FCER1a Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Val26-Gln205.[Accession | P12319-1]

分子量大小（Molecular Weight）

The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and HPLC

活性（Activity）

ELISA Data: Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 10ng/ml determined by ELISA. 

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

Ver. CN20230625

重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

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重组人FcεRIα/FCER1a蛋白(hFc标签) Human Fc epsilon RI alpha/FCER1a Protein

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重组食蟹猴Fc-gamma RIIB蛋白(His标签) Cynomolgus Fc gamma RIIB Protein

发表于由

重组食蟹猴Fc-gamma RIIB蛋白(His标签) Cynomolgus Fc gamma RIIB Protein

产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统（Source）

Cynomolgus Fc gamma RIIB Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ala46-Pro224.[Accession | Q8SPW3]

分子量大小（Molecular Weight）

The protein has a predicted MW of 21 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
活性（Activity）

SPR Data: Trastuzumab captured on CM5 Chip via Protein A can bind Cynomolgus Fc gamma RIIB, His Tag with an affinity constant of 2.72 μM as determined in SPR assay (Biacore T200).

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.           

Ver. CN20230620

重组食蟹猴Fc-gamma RIIB蛋白(His标签) Cynomolgus Fc gamma RIIB Protein

暂无内容

重组食蟹猴Fc-gamma RIIB蛋白(His标签) Cynomolgus Fc gamma RIIB Protein

暂无内容

性能参数

表达区间及表达系统（Source）

Cynomolgus Fc gamma RIIB Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ala46-Pro224.[Accession | Q8SPW3]

分子量大小（Molecular Weight）

The protein has a predicted MW of 21 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
活性（Activity）

SPR Data: Trastuzumab captured on CM5 Chip via Protein A can bind Cynomolgus Fc gamma RIIB, His Tag with an affinity constant of 2.72 μM as determined in SPR assay (Biacore T200).

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.

2-7 days, 2 ~8 °C under sterile conditions after reconstitution.

3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.           

Ver. CN20230620

重组食蟹猴Fc-gamma RIIB蛋白(His标签) Cynomolgus Fc gamma RIIB Protein

暂无内容

重组食蟹猴Fc-gamma RIIB蛋白(His标签) Cynomolgus Fc gamma RIIB Protein

暂无内容

athensresearch 16-16-090707-1M-FC

发表于由

athensresearch 16-16-090707-1M-FC说明书

Immunoglobulin G1 (IgG1), Human Myeloma Plasma, FC Fragment

免疫球蛋白G1（IgG1），人骨髓瘤血浆，FC片段
尺寸许可数量
1毫克$230.00

产品编号：16-16-090707-1M-FC
打印页面电子邮件页面链接
规格
直接从制造商处购买。
批量可用。
分子量：50000 Da，通过SDS-PAGE
消光系数：1.35
在50 mM Tris HCl中冷冻，pH 8.0，200 mM NaCl和0.05%NaN3。
由两个免疫球蛋白重链的羧基末端通过二硫键相互连接而成的可结晶片段。Fc片段包含负责免疫球蛋白效应器功能的重链恒定区的羧基末端部分（补体固定、通过Fc受体与细胞膜结合和胎盘转运）。IgG1 FC作为一种潜在的抗炎药在治疗人类自身免疫性疾病中有着新的作用。
Anthony R，Ravetch J等人。（2010），临床免疫学杂志。30补遗1:S9-14
IgG1-FC片段不与抗原结合，但在细胞营养反应、补体结合和分解代谢率控制方面，它包含经典的抗原决定簇和生物活性。
储存温度：-20°C
纯度：SDS-PAGE法大于等于95%。
由经FDA批准的HBsAg、抗HCV、抗HBc和抗HIV 1&amp;2阴性的血浆制备。
雅典研究与技术产品是实验室试剂，不得用于人类或任何药物用途。仅用于研究或进一步制造。

 

 

 

 

16-16-090707-LC

Immunoglobulin G (IgG), Light Chain, Human Plasma

16-16-090707

Immunoglobulin G (IgG), Normal Human Plasma

16-16-090707-dog

Immunoglobulin G, Dog Plasma

16-16-090707-FAB2

Immunoglobulin G, Fab '2, Human Plasma

16-16-090707-Fab

Immunoglobulin G, Fab Fragment, Human Plasma

16-16-090707-FC

Immunoglobulin G, FC Fragment, Human Plasma

16-16-090707-FC-IMM

Immunoglobulin G, FC Fragment, Human Plasma, Immunogen Grade

16-16-090707-FC-LEL

Immunoglobulin G, FC Fragment, Low Endotoxin Level, Human Plasma

16-16-090707-mouse

Immunoglobulin G, Mouse Plasma

16-16-090707-RAT

Immunoglobulin G, Rat Serum

16-16-090707-1M

Immunoglobulin G1 (IgG1), Human Myeloma Plasma

16-16-090707-1M-FAB

Immunoglobulin G1 (IgG1), Human Myeloma Plasma, Fab Fragment

16-16-090707-1M-FC

Immunoglobulin G1 (IgG1), Human Myeloma Plasma, FC Fragment

16-16-090707-1

Immunoglobulin G1 (IgG1), Normal Human Plasma

16-16-090707-2M

Immunoglobulin G2 (IgG2), Human Myeloma Plasma

16-16-090707-2

Immunoglobulin G2 (IgG2), Normal Human Plasma

16-16-090707-3

Immunoglobulin G3 (IgG3), Normal Human Plasma

16-16-090707-4M

Immunoglobulin G4 (IgG4), Human Myeloma Plasma

16-16-090707-4

Immunoglobulin G4 (IgG4), Normal Human Plasma

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

发表于由

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

产品说明书

FAQ

COA

已发表文献

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. Glycosylation of the EPO protein is required for biological activities in vivo . The mature human EPO protein shares 75% – 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells . Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations.

 

Product Properties

Synonyms

Erythropoietin, Epoetin

Accession

P01588

Gene ID

2056

Source

HEK293 Cells-derived human EPO protein, Met1-Arg193 with hFc Tag at the C-terminus

Molecular Weight

Approximately 45.1 kDa.

AA Sequence

MGVHECPAWL WLLLSLLSLP LGLPVLGAPP RLICDSRVLE RYLLEAKEAE NITTGCAEHC SLNENITVPDTKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQALLVN SSQPWEPLQL HVDKAVSGLR SLTTLLRALG AQKEAISPPD AASAAPLRTI TADTFRKLFR VYSNFLRGKL KLYTGEACRT GDR

Tag

hFc Tag

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE.

Biological Activity

1. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2.0-8.0 ng/mL.
2. Measured by its ability to bind Human EPOR (ECD, His Tag) in functional ELISA.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only.

 

 

HB220415

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

暂无内容

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

暂无内容

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species. Glycosylation of the EPO protein is required for biological activities in vivo . The mature human EPO protein shares 75% – 84% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain. EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the erythropoietin receptor (EPO R). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells . Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation. Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations.

 

Product Properties

Synonyms

Erythropoietin, Epoetin

Accession

P01588

Gene ID

2056

Source

HEK293 Cells-derived human EPO protein, Met1-Arg193 with hFc Tag at the C-terminus

Molecular Weight

Approximately 45.1 kDa.

AA Sequence

MGVHECPAWL WLLLSLLSLP LGLPVLGAPP RLICDSRVLE RYLLEAKEAE NITTGCAEHC SLNENITVPDTKVNFYAWKR MEVGQQAVEV WQGLALLSEA VLRGQALLVN SSQPWEPLQL HVDKAVSGLR SLTTLLRALG AQKEAISPPD AASAAPLRTI TADTFRKLFR VYSNFLRGKL KLYTGEACRT GDR

Tag

hFc Tag

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE.

Biological Activity

1. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 2.0-8.0 ng/mL.
2. Measured by its ability to bind Human EPOR (ECD, His Tag) in functional ELISA.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at –20℃ to -80℃ for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only.

 

 

HB220415

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

暂无内容

Human EPO/Fc 重组人促红细胞生成素Fc(人促红细胞生成素Fc)

暂无内容

lifelinecelltech FC-0079说明书

发表于由

 

 

lifelinecelltech FC-0079说明书

Lifeline®细胞技术的创始人在25年前帮助引入了正常人类细胞系统和培养基进行研究。Lifeline®通过细致的质量测试、不断的创新以及提供*佳客户和技术服务的热情，Lifeline®细胞技术（Lifeline™）专门开发和生产纯化的原代人细胞和用于细胞培养的优化试剂。

Lifeline®科学家已经在这一重要的细胞培养领域工作了20多年

Lifeline®科学家开发了许多人类细胞系统和质量标准，这些系统和标准目前用于学术、政府和制药实验室研究人类疾病

我们的科学家和质量控制技术人员团队继续制造新的和改进的人类细胞系统，以满足对原代人类细胞要求更高的细胞培养市场的需求

Lifeline®科学家正在人类干细胞产品开发方面取得突破

Human Bladder Dome Epithelial Cells

人膀胱上皮细胞

SKU: FC-0079

Categories: Bladder, Bladder

GTIN: 1246000000000

 

产品描述

当在UroLifeTM培养基中生长时，Lifeline®正常人膀胱穹顶上皮细胞为膀胱生物学研究提供了理想的培养模型。

获得*佳研究结果的原代膀胱细胞

原代膀胱上皮细胞——穹窿是从人膀胱组织的穹窿区分离出来的，并在培养容器中扩增，然后采集进行冷冻保存。这些膀胱细胞是直接从其来源器官组织中培养出来的，没有经过任何修饰，并且来自各种捐献者——所有这些都有助于确保有效的研究结果。

膀胱细胞圆顶培养我们的膀胱上皮细胞在UroLifeTM培养基中进行质量测试，以确保正确的生长和形态。

当在Lifeline®培养基中培养时，Lifeline™膀胱上皮细胞无需暴露于抗生素或酚红。Lifeline®提供了这些传统补充剂，但在大多数研究应用中，不需要或不建议使用这些补充剂来实现*佳细胞性能（这是一个优势，因为这些补充品可能会对实验结果产生负面影响）。

排泄系统细胞：每瓶500000个细胞

测试人膀胱穹顶上皮细胞：

细胞存活率：从低温保存解冻时，存活率大于70%

增殖和形态：15个群体倍增的正常细胞外观

无菌检测：支原体阴性。细菌和真菌生长阴性。

病毒检测：PCR检测HIV-1、HIV-2、HBV和HCV阴性