标签归档：fc

lifelinecelltech FC-0079说明书

发表于由

 

 

lifelinecelltech FC-0079说明书

Lifeline®细胞技术的创始人在25年前帮助引入了正常人类细胞系统和培养基进行研究。Lifeline®通过细致的质量测试、不断的创新以及提供*佳客户和技术服务的热情，Lifeline®细胞技术（Lifeline™）专门开发和生产纯化的原代人细胞和用于细胞培养的优化试剂。

Lifeline®科学家已经在这一重要的细胞培养领域工作了20多年

Lifeline®科学家开发了许多人类细胞系统和质量标准，这些系统和标准目前用于学术、政府和制药实验室研究人类疾病

我们的科学家和质量控制技术人员团队继续制造新的和改进的人类细胞系统，以满足对原代人类细胞要求更高的细胞培养市场的需求

Lifeline®科学家正在人类干细胞产品开发方面取得突破

Human Bladder Dome Epithelial Cells

人膀胱上皮细胞

SKU: FC-0079

Categories: Bladder, Bladder

GTIN: 1246000000000

 

产品描述

当在UroLifeTM培养基中生长时，Lifeline®正常人膀胱穹顶上皮细胞为膀胱生物学研究提供了理想的培养模型。

获得*佳研究结果的原代膀胱细胞

原代膀胱上皮细胞——穹窿是从人膀胱组织的穹窿区分离出来的，并在培养容器中扩增，然后采集进行冷冻保存。这些膀胱细胞是直接从其来源器官组织中培养出来的，没有经过任何修饰，并且来自各种捐献者——所有这些都有助于确保有效的研究结果。

膀胱细胞圆顶培养我们的膀胱上皮细胞在UroLifeTM培养基中进行质量测试，以确保正确的生长和形态。

当在Lifeline®培养基中培养时，Lifeline™膀胱上皮细胞无需暴露于抗生素或酚红。Lifeline®提供了这些传统补充剂，但在大多数研究应用中，不需要或不建议使用这些补充剂来实现*佳细胞性能（这是一个优势，因为这些补充品可能会对实验结果产生负面影响）。

排泄系统细胞：每瓶500000个细胞

测试人膀胱穹顶上皮细胞：

细胞存活率：从低温保存解冻时，存活率大于70%

增殖和形态：15个群体倍增的正常细胞外观

无菌检测：支原体阴性。细菌和真菌生长阴性。

病毒检测：PCR检测HIV-1、HIV-2、HBV和HCV阴性

 

重组生物素化人FcγRIIA/CD32a(H167)蛋白(His-Avi标签) Human Fc gamma RIIA/CD32a (H167) Protein

发表于由

重组生物素化人FcγRIIA/CD32a(H167)蛋白(His-Avi标签) Human Fc gamma RIIA/CD32a (H167) Protein

产品说明书

FAQ

COA

已发表文献

性能参数

表达区间及表达系统（Source）

Recombinant Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(H167).[Uniprot  | P12318-1]

分子量大小

（Molecular Weight）

The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and  HPLC

活性（Activity）

SPR Data: Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 1.44 μM as determined in SPR assay .

SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.680 μM as determined in SPR assay

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.  2-7 days, 2 ~8 °C under sterile conditions after reconstitution.   3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.                  

 

Ver. CN20230421

重组生物素化人FcγRIIA/CD32a(H167)蛋白(His-Avi标签) Human Fc gamma RIIA/CD32a (H167) Protein

暂无内容

重组生物素化人FcγRIIA/CD32a(H167)蛋白(His-Avi标签) Human Fc gamma RIIA/CD32a (H167) Protein

暂无内容

性能参数

表达区间及表达系统（Source）

Recombinant Biotinylated Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(H167).[Uniprot  | P12318-1]

分子量大小

（Molecular Weight）

The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by Tris-Bis PAGE and  HPLC

活性（Activity）

SPR Data: Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 1.44 μM as determined in SPR assay .

SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.680 μM as determined in SPR assay

制剂（Formulation）

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~–15℃ for 1 year from date of receipt.  2-7 days, 2 ~8 °C under sterile conditions after reconstitution.   3 months, -25~–15℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.                  

 

Ver. CN20230421

重组生物素化人FcγRIIA/CD32a(H167)蛋白(His-Avi标签) Human Fc gamma RIIA/CD32a (H167) Protein

暂无内容

重组生物素化人FcγRIIA/CD32a(H167)蛋白(His-Avi标签) Human Fc gamma RIIA/CD32a (H167) Protein

暂无内容

FormuMax F70101C-N 说明书

发表于由

世界*实验材料供应商 FormuMax上海金畔生物为其中国代理， FormuMax在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， FormuMax就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

FormuMax中国代理, FormuMax上海代理， FormuMax北京代理，FormuMax广东代理， FormuMax江苏代理FormuMax湖北代理,FormuMax天津,FormuMax黑龙江代理,FormuMax内蒙古代理,FormuMax吉林代理,FormuMax福建代理, FormuMax江苏代理, FormuMax浙江代理, FormuMax四川代理,

 

FormuMax Scientific是一家位于加州北部硅谷中心提供脂质体给药的专业公司，以满足制药、生物技术、化妆品及营养企业日益增长的研发需求。FormuMax Scientific力求成为脂质体给药领域的，特别是在配制用脂质体及其他特定纳米包埋传输的复杂药物领域。在药物传输企业，FormuMax Scientific拥有由世界*专家的技术团队，并致力于脂质体及其它以磷脂为基础的先进药物传输技术。FormuMax 是目前*一家在线提供大量预制脂质体试剂的公司，可为大学、研究所、研究中心及各种研发企业提供的药物传输试剂。同时该公司还可为基础研究、配方可行性、定制配方制备、配方鉴定及分析提供技术服务。

 

FormuMax供应主要产品：

Clophosome® –氯膦酸盐脂质体

FormuMax提供两种预埋有氯磷酸盐分子的中性脂质体产品，该产品单次静脉或腹腔注射后可在脾脏经巨噬细胞清除率可达80-90%，并具有高度活性、稳定的理化性质（长期冰箱存放后仍保持均一性及悬浮特性），粘滞度低使用简便，适用于静脉、腹腔、皮下、鼻腔、气管等多种方式给药。

Doxoves®-盐酸多柔比星脂质体

FormuMax可提供脂质定制的盐酸多柔比星脂质体（定制内容为脂质成分及脂质体大小）。FormuMax提供的产品为Doxoves®，具有高度的产品稳定性和一致性, 紧密的粒径分布和的药物包埋效率（高达4.0 mg/mL(40mM lipid)的包埋药物浓度）。 Doxoves®系列产品在产品特性、稳定性及药代动力学特性上可媲美商业产品Doxil® 。

Drug Loaded Liposomes

FormuMax提供多款包埋活性药物的脂质体产品，包括Doxorubicin HCl（含2.0mg/mL及4.0mg/mL两种浓度），Irinotecan HCl (2.0mg/mL)，Topotecan HCl (2.0mg/mL)，产品线仍在持续更新中。

预制脂质体

FormuMax 供应的预制脂质体产品囊括多种通用的脂质体类型，使用世界*的脂类原料。该系列产品易于使用，稳定性、可靠性及可重复性高，节省时间和成本，并可为研究人员提供定制脂质体服务。目前FormuMax提供聚乙二醇化/非聚乙二醇化的常用脂质体，以及含浓度梯度Ammonium的脂质体

阳离子脂质体

FormuMax供应一系列的阳离子脂质体，用于需带正电荷的脂质体或细胞转染质粒DNA、反义/干扰RNA等。 

荧光脂质体

FormuMax 供应预制荧光标记的脂质体，包括常规荧光脂质体、生物素化的荧光脂质体以及Avdinized化的荧光脂质体，另外还提供客户定制的荧光脂质体（脂类及染料可选）。

脂质体制备薄膜

FormuMax 供应一系列不同规格的聚碳酸酯薄膜，用于脂质体挤出制备。

 

Clophosome® – Clodronate Liposomes (Neutral)

SKU: F70101C-N

* Volume:

  • 2 mL
  • 10mL

Product Description

Clophosome®– Clodronate Liposomes (Neutral)

Clophosome®, neutral clodronate liposomes, offers highly efficient in vivo macrophage depletion. Clodronate molecules are encapsulated in small MLV liposomes for superior activity, physical and chemical stability, and convenience of use. It is formulated to an exceptionally high clodronate/lipid ratio. Free clodronate is cleaned out and the liposomal clodronate encapsulation efficiency is more than 90%. Clophosome liposomes remain homogenous and suspended even after long term storage in fridge. It is not viscous and easy to inject. It is manufactured sterile for long term shelf-life. 

The product is suitable for dosing with various routes including intravenous, intraperitoneal, subcutaneous, intranasal, intratracheal and etc. We have achieved 80-90% macrophage depletion in spleen (red pulp macrophages) 24 hours after a single 0.2 mL intravenous injection. A starting dose of 0.15 – 0.2 mL (i.v. or i.p.) for 20-25g animal body weight is recommended for good consistency in macrophage depletion. Optimization is recommended for your specific applications.

 

Specifications

Product code: F70101C-N 

Brand Name: Clophosome®

Active: clodronate disodium. CAS: 22560-50-5, 7 mg/mL

 

Lipid composition: phosphatidylcholine and cholesterol

Bulk solution: phosphate buffered saline (20mM sodium phosphate, 09wt% NaCl), pH 7.0-7.5

Shelf-life: 6 months guaranteed for unopened vials

 

部分产品报价如下：

货号 品名 规格 价格 品牌
F90101TH-05 Trans-Hi™ – In vitro Transfection Reagent 0.5 1935 FormuMax Scientific2017
F90101TH-10 Trans-Hi™ – In vitro Transfection Reagent 1.0 3435 FormuMax Scientific2017
F90101TH-101 Trans-Hi™ – In vitro Transfection Reagent 10 x 1.0mL 25485 FormuMax Scientific2017
F90101TH-00 free evaluation sample, Trans-Hi™ – In vitro Transfection Reagent (50µl) 0.05 400 FormuMax Scientific2017
F10102 Plain DPPC/CHOL Liposomes (55:45 mol/mol, 100 nm, 60mM) 10 7485 FormuMax Scientific2017
F10103 Plain DSPC/CHOL Liposomes (55:45 mol/mol, 100 nm, 60mM) 10 5235 FormuMax Scientific2017
F10104 Plain HSPC/CHOL Liposomes (55:45 mol/mol, 100 nm,60mM) 10 4485 FormuMax Scientific2017
F10105 Plain 20:0PC/CHOL Liposomes (55:45 mol/mol, 100 nm, 60mM) 10 7485 FormuMax Scientific2017
F10109 Plain Pure DOPC Liposomes (100 nm, 60mM) 10 5385 FormuMax Scientific2017
F10110 Plain Pure POPC Liposomes (100 nm, 60mM) 10 6285 FormuMax Scientific2017
F10202 Plain DPPC/CHOL/mPEG2000-DSPE Liposomes (50:45:5 mol/mol, 100 nm, 60mM) 10 8235 FormuMax Scientific2017
F10203 Plain DSPC/CHOL/mPEG2000-DSPE Liposomes (50:45:5 mol/mol, 100 nm, 60mM) 10 6285 FormuMax Scientific2017
F10204 Plain HSPC/CHOL/mPEG2000-DSPE Liposomes (50:45:5 mol/mol, 100 nm, 60mM) 10 5685 FormuMax Scientific2017
F10205 Plain 20:0PC/CHOL/mPEG2000-DSPE Liposomes (50:45:5 mol/mol, 100 nm, 60mM) 10 8235 FormuMax Scientific2017
F20103A DSPC/CHOL (55:45 mol/mol)  Liposomes with Ammonium Gradient (100nm) 10 8385 FormuMax Scientific2017
F20104A HSPC/CHOL (55:45 mol/mol)  Liposomes with Ammonium Gradient (100nm) 10 7935 FormuMax Scientific2017
F20203A DSPC/CHOL/mPEG-DSPE (50:45:5 mol/mol)  Liposomes with Ammonium Gradient (100nm) 10 8985 FormuMax Scientific2017
F20204A HSPC/CHOL/mPEG2000-DSPE (50:45:5 mol/mol) Liposomes with Ammonium Gradient (100nm) 10 8235 FormuMax Scientific2017
F20204AB HSPC/CHOL/mPEG2000-DSPE (56.2/38.5/5.3) Liposomes with Ammonium Gradient (100nm) 10 8835 FormuMax Scientific2017
F20204AS SUV HSPC/CHOL/mPEG2000-DSPE (56.2/38.5/5.3) Liposomes with Ammonium Gradient (80 nm) 10 10485 FormuMax Scientific2017
F30204B-D2-2 Doxoves® – STEALTH Liposomal Doxorubicin HCl (2.0mg/mL)  2 4485 FormuMax Scientific2017
F30204B-D2-10 Doxoves® – STEALTH Liposomal Doxorubicin HCl (2.0mg/mL)  10 14985 FormuMax Scientific2017
F30204B-C2-2 Control liposomes for Doxoves® (20mM) 2 2235 FormuMax Scientific2017
F30204B-C2-10 Control liposomes for Doxoves® (20mM) 10 7485 FormuMax Scientific2017
F30204C-D2-2 Combo kit of Doxoves® (2.0mg/mL) Control liposomes for Doxoves® (20mM) 2 5985 FormuMax Scientific2017
F30204C-D2-10 Combo kit of Doxoves® (2.0mg/mL) Control liposomes for Doxoves® (20mM) 10 20235 FormuMax Scientific2017
F30204B-D-2 Doxoves® – STEALTH Liposomal Doxorubicin HCl (4.0mg/mL)  2 7035 FormuMax Scientific2017
F30204B-D-10 Doxoves® – STEALTH Liposomal Doxorubicin HCl (4.0mg/mL)  10 23985 FormuMax Scientific2017
F30204C-D-2 Combo kit of Doxoves®  and plain control liposomes 2 9285 FormuMax Scientific2017
F30204C-D-10 Combo kit of Doxoves®  and plain control liposomes 10 32385 FormuMax Scientific2017
F30204B-C-2 Control liposomes for Doxoves® (40mM) 2 2835 FormuMax Scientific2017
F30204B-C-10 Control liposomes for Doxoves® (40mM) 10 11535 FormuMax Scientific2017
F30204-IT-2 STEALTH Liposomal Irinotecan HCl (2.0mg/mL, 80-100nm)  2 11985 FormuMax Scientific2017
F30204-TT-2 STEALTH Liposomal Topotecan HCl (2.0mg/mL, 80-100nm)  2 11985 FormuMax Scientific2017
FB30020 Dilution Buffer for Doxoves®  20 885 FormuMax Scientific2017
F50101 Cationic Liposomes DMTAP/DOPE 2 4335 FormuMax Scientific2017
F50102 Cationic Liposomes DOTAP/DOPE 2 3285 FormuMax Scientific2017
F50103 Cationic Liposomes DDAB/DOPE 2 3435 FormuMax Scientific2017
F50104 Cationic Liposomes DODAP/DOPE 2 3585 FormuMax Scientific2017
F50105 Cationic Liposomes DC-CHOL/DOPE 2 3585 FormuMax Scientific2017
F60103-P Placebo Control for fluorescent DOPC/CHOL Liposomes 1 2685 FormuMax Scientific2017
F60103F-D DOPC/CHOL Liposomes labeled with dansyl DOPE 1 5985 FormuMax Scientific2017
F60103F-F  DOPC/CHOL Liposomes labeled with Fluorescein DHPE 1 4935 FormuMax Scientific2017
F60103F-MB DOPC/CHOL Liposomes labeled with Marina Blue-DHPE 1 8535 FormuMax Scientific2017
F60103F-NBD DOPC/CHOL Liposomes Labeled with NBD-PE 1 4935 FormuMax Scientific2017
F60103F-OG DOPC/CHOL Liposomes labeled with Oregon Green 488 DHPE 1 6585 FormuMax Scientific2017
F60103F-R DOPC/CHOL Liposomes labeled with Rhodamine DHPE 1 4935 FormuMax Scientific2017

 

AB Biosciences Fc受体家族产品

发表于由

ABB的核心能力是用于免疫肿瘤疾病的诊断和治疗应用的生物制剂蛋白质设计。具体而言，我们的疗法研发专注于能够调节与抗原靶标和效应分子相关的活性的生物制剂。此外，ABB设计并生产了一组与免疫相关的蛋白质试剂，可供生物医学研究人员用作研究工具。

历史

AB Biosciences（ABB）成立于2007年，一直致力于治疗生物制剂和研究蛋白试剂的工程设计。凭借数十年的研发经验，ABB的科学家们为开发这两种蛋白质类别建立了一个有效的平台。传统IVIG（PRIM程序）的重组替代是我们在研发*蛋白药物以替代传统血清衍生IVIG方面的工程专业技术的一个很好的例子。

AB Biosciences Fc受体家族产品

蛋白质名称 种类 融合标签 目录代码
FcRn 人的 谷胱甘肽S转移酶（GST） P7142D
FcγRI 人的 谷胱甘肽S转移酶（GST） P7131D
FcγRIIa，H131 人的 谷胱甘肽S转移酶（GST） P7501D
FcγRIIa，R131 人的 谷胱甘肽S转移酶（GST） P7133D
FcγRIIb 人的 谷胱甘肽S转移酶（GST） P7134D
FcγRIII，F158 人的 谷胱甘肽S转移酶（GST） P7135D
FcγRIII，V158 人的 谷胱甘肽S转移酶（GST） P7502D
FcγRI 老鼠 谷胱甘肽S转移酶（GST） M7131D
FcγRIIb 老鼠 谷胱甘肽S转移酶（GST） M7134D
FcγRIII 老鼠 谷胱甘肽S转移酶（GST） M7135D
丙型肝炎病毒 老鼠 谷胱甘肽S转移酶（GST） M7511D

抗体是双功能分子。抗体的前半部分包含识别抗原的可变区（Fab）。抗体的后半部分包含与一系列Fc受体（FcR）相互作用的可结晶片段（Fc）。Fc-FcR相互作用将结合Fab的抗原带到免疫效应细胞附近。FcR主要以它们结合的抗体种类命名：Fcγ受体（FcγR）与IgG结合，Fcα受体（FcaRs）与IgA结合，Fcε受体（FceRs）与IgE结合，Fc mu受体（FcmR）结合IgM和IgA。除经典（与Ig超家族结构相关）受体外，IgG还与两种非经典受体结合，即新生儿Fc受体（FcRn）和DC-SIGN。

常规FcR属于免疫球蛋白超家族。在人类中，该家族包括五个成员，FcγRI，FcγRIIA，FcγRIIB，FcγRIIIA和FcγRIIIB。在小鼠，该家族包括4名成员，FC γ RI，FcγRIIB的，FcγRIII的，和FcγRIV。间的非规范的Fc γ受体，FcRn的在结构上与I类主要组织相容性复合物（MHC-1），和DC-SIGN（在SIGNR1小鼠）是有关C型lections。

除FcγRIIB和FcgRIIIB外，所有规范的人Fcγ受体均通过固有的（FcγRIIA）或与Fcγ链相关的ITAM基序（FcγRI和FcγRIIIA）转导激活信号。相比之下，FcγRIIB通过固有的ITIM基序转导抑制信号，而FcγRIIIB则缺乏跨膜区和细胞内结构域。相反，它被GPI锚定在细胞表面，被认为是诱饵受体。FcRn和DC-SIGN中均不存在细胞内结构域。在小鼠中，所有激活受体（FcγRI，FcγRIII和FcγRIV）通过相关Fcγ链中的ITAM基序转导激活信号，而小鼠FcγRIIB通过内在ITIM基元转导抑制信号。FcRn和SIGNR1都不转导细胞内信号，因为两者都缺乏细胞内结构域。

丰富的抗体类别人IgG 1以高亲和力与FcγRI和FcRn结合，并以低亲和力与FcγRIIA，FcγRIIB，FcγRIIIA，FcγRIIIB和DC-SIGN结合。这样，仅IgG-抗原免疫复合物可以通过多价相互作用（亲和力结合）有效地结合这些受体。同样，小鼠中抗体种类多的小鼠IgG 2a以高亲和力与FcgRI和FcRn结合，与抗原复合后仅与FcγRIIB，FcγRIII，FcγRIV和SIGNR1结合。

人IgG 1和小鼠IgG 2a都在Asn297残基处被糖基化。该聚糖部分的细微变化会显着影响IgG与Fcγ受体的结合。例如，岩藻糖基部分部分地阻止了IgG与FcγRIII（人）和FcγRIV（小鼠）的结合。由于缺少这种岩藻糖的抗体（岩藻糖基抗体）可以与这些受体结合，并具有更高的亲和力，因此已经探索出治疗性抗体的更好疗效。

 

 

 

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

发表于由

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

产品说明书

FAQ

COA

已发表文献

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities only recognizing IgG aggregates surrounding multivalent antigens. Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta. The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses.

 

产品性质

别名（Synonyms）

CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI

Uniprot No.

 P12314-1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RI/CD64 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln16-Pro288. 

分子量(Molecular Weight)

The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data: Human Fc gamma RI, His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 11.3nM as determined in SPR assay.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

HB220624

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

暂无内容

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

暂无内容

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities only recognizing IgG aggregates surrounding multivalent antigens. Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta. The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses.

 

产品性质

别名（Synonyms）

CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI

Uniprot No.

 P12314-1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RI/CD64 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln16-Pro288. 

分子量(Molecular Weight)

The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data: Human Fc gamma RI, His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 11.3nM as determined in SPR assay.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

HB220624

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

暂无内容

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

暂无内容

FC-MicroPlus-迷你台式高速离心机TC-MicroSpin

发表于由

FC-MicroPlus迷你台式高速离心机TC-MicroSpin

品牌 加工定制 否
结构类型 台式 布局形式 其他

迷你台式高速离心机TC-MicroSpin/FC-MicroPlus，
容量：12 x 1.5 ml / 2 ml 的离心管
转速 14,500 rpm；相对离心力 14,100 x g
简单直观，便于操作的数字显示屏
超静音操作
金属转子

迷你台式高速离心机TC-MicroSpin/FC-MicroPlus

产品特点

  • 精巧的造型

  • 大容量：12 x 1.5 ml / 2 ml 的离心管

  • 大转速 14,500 rpm；大相对离心力 14,100 x g

  • 到达大转速的加速时间和低转速的减速时间<13秒

  • 简单直观，便于操作的数字显示屏

  • 超静音操作

  • 金属转子

  • 符合IEC 1010-2-020安全认证

  • 单独的short 瞬时离心按键，实现快速便捷地瞬时离心

  • 的气流交换技术，保护温度敏感性样品

  • 离心结束后，自动开盖，减少操作时间

  • 免维护电机

  • 转子可高温高压灭菌（121°C，20 分钟）

  • 转子和盖子耐化学腐蚀           FC-MicroPlus-迷你台式高速离心机TC-MicroSpin

技术参数

型号

FC-MicroSpin

FC-MicroPlus

电源

120V-60Hz or 230V-50HZ

120V-60Hz or 230V-50HZ

电源功率

100W

105W

大转速

800-13,400 rpm
 (in 100 rpm increments)

800-14,500 rpm
(in 100 rpm increments)

大相对离心率

12,100 x g

14,100 x g

时间设定范围

15 sec—99min

15 sec—99min

加速至大转速的时间

13s

13s

从大转速减速的时间

12s

128s

尺寸（长X宽X高）

26x26x15

26x26x15

重量（包括转子）

4.3kg

4.3kg

订货信息

产品货号

产品编号

规格（大转速）

8011151

TC-MicroSpin

13,400 rpm/min

8011152

TC-MicroPlus

14,500 rpm/min

转子及附件

  • 1. 透明高强度塑料盖

  • 2. 转子可高温高压灭菌（121℃，20min）

  • 3. 可选购适配器配件
    —- 0.2ml PCR管的适配器
    —- 0.5ml PCR管的适配器

迷你台式高速离心机TC-MicroSpin/FC-MicroPlus

FC-MicroPlus-迷你台式高速离心机TC-MicroSpinFC-MicroPlus-迷你台式高速离心机TC-MicroSpin

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

发表于由

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

产品说明书

FAQ

COA

已发表文献

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands.

 

产品性质

别名（Synonyms）

Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A

Uniprot No.

 P12318-1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(H167).

分子量(Molecular Weight)

The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data：Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.606 μM as determined in SPR assay.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

HB220624

 

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

暂无内容

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

暂无内容

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC).The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands.

 

产品性质

别名（Synonyms）

Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A

Uniprot No.

 P12318-1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218(H167).

分子量(Molecular Weight)

The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data：Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.606 μM as determined in SPR assay.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

HB220624

 

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

暂无内容

重组人FcγRIIA/CD32a(H167)(His-Avi标签)|Recombinant Human Fc gamma RIIA/CD32a

暂无内容

重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

发表于由

重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

产品说明书

FAQ

COA

已发表文献

 

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16). Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified. The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

 

产品性质

别名

Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A

Uniprot No.

P12318.1

表达区间及表达系统

Recombinant Human Fc gamma RIIA/CD32a (R167) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218.

分子量

Approximately 23.2 kDa. Due to glycosylation, the protein migrates to 34-40 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

SPR Data：Human Fc gamma RIIA (R167) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 9μM as determined in SPR assay (Biacore T200).

内毒素

< 1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

 

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据
重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白
重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

HB220810

 

重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

暂无内容

重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

暂无内容

 

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16). Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified. The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.

 

产品性质

别名

Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A

Uniprot No.

P12318.1

表达区间及表达系统

Recombinant Human Fc gamma RIIA/CD32a (R167) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala36-Ile218.

分子量

Approximately 23.2 kDa. Due to glycosylation, the protein migrates to 34-40 kDa based on Tris-Bis PAGE result.

纯度

> 95% as determined by SDS-PAGE and HPLC.

活性

SPR Data：Human Fc gamma RIIA (R167) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 9μM as determined in SPR assay (Biacore T200).

内毒素

< 1.0 EU per 1μg of the protein by the LAL method.

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

 

1. 避免反复冻融。

2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。

3. 本产品仅作科研用途！

 

产品数据
重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白
重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

HB220810

 

重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

暂无内容

重组人Fc γ RIIA/CD32a(R167)(His-Avi标签)免疫蛋白

暂无内容

affinitybiologicals F8C-EIA说明书

发表于由

affinitybiologicals F8C-EIA说明书

 

Factor VIII Paired Antibody Set

 

VIII因子配对抗体组

 

F8C-EIA –因子VIII：C配对抗体组– 4板组

分类：抗体，配对抗体组，VIII因子产品，VIII因子抗体，VIII因子EIA

 

描述

VIII因子配对抗体组

Affinity的VIII因子配对抗体组由匹配的捕获和检测抗体组成，这些抗体经过滴定和优化后可用于三明治式ELISA分析。提供的产品包含足够的捕获和检测抗体，可用于四个完整的96孔微孔板，并且包含详细的协议表，其中包含使用说明，溶液配方和所需其他材料的来源。VIII因子配对抗体组旨在帮助终用户建立“内部”免疫测定法，仅用于研究目的，不得用于诊断应用。分析验证是终用户的责任。

产品编号： F8C-EIA

随附材料：

  1. 捕获抗体（F8C-EIA-C）：一个黄色封闭的小瓶，其中包含0.4 ml多克隆亲和纯化抗因子VIII抗体，用于包被板。
  2. 检测抗体（F8C-EIA-D）：一根装有0.4 ml过氧化物酶结合的亲和纯化的多克隆抗因子VIII抗体的红盖试管，用于检测捕获的因子FVIII。