重组食蟹猴Fc-gamma RIIB蛋白(His标签) Cynomolgus Fc gamma RIIB Protein
产品说明书
FAQ
COA
已发表文献
性能参数
表达区间及表达系统(Source) |
Cynomolgus Fc gamma RIIB Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ala46-Pro224.[Accession | Q8SPW3] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 21 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Tris-Bis PAGE |
活性(Activity) |
SPR Data: Trastuzumab captured on CM5 Chip via Protein A can bind Cynomolgus Fc gamma RIIB, His Tag with an affinity constant of 2.72 μM as determined in SPR assay (Biacore T200). |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution) |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~–15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~–15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230620
性能参数
表达区间及表达系统(Source) |
Cynomolgus Fc gamma RIIB Protein is expressed from HEK293 with His tag at the C-terminal. It contains Ala46-Pro224.[Accession | Q8SPW3] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 21 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by Tris-Bis PAGE |
活性(Activity) |
SPR Data: Trastuzumab captured on CM5 Chip via Protein A can bind Cynomolgus Fc gamma RIIB, His Tag with an affinity constant of 2.72 μM as determined in SPR assay (Biacore T200). |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution) |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~–15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~–15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Ver. CN20230620
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