Enzymatics N2050-10-L说明书

发表于2024年7月6日由上海金畔生物科技有限公司

货号：N2050-10-L

品名：10 mM dNTP Solution Mix

产品描述：

四种天然脱氧核苷酸的等摩尔（10mM）混合物。

规格：	100 umol
浓度：	10 mM
保存：	-25°C至-15°C
纯度	>99.9％
碱基纯度	99.5%
焦磷酸	0.003 pmol/pmol of nucleotide
ATP	未检测到
Nick 活性	未检测到
DNase活性	未检测到
RNase活性	未检测到

提供：
pH 7.5, 各10mM dNTP（dATP，dCTP，dGTP，dTTP）

部分产品信息：

货号	品名	规格	浓度	品牌
N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

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Enzymatics Y9410L 产品说明书

发表于2024年7月5日由上海金畔生物科技有限公司

货号：Y9410L

品名：5X WGS Fragmentation Mix（5X WGS碎片混合物）

产品描述：

这种组合为Illumina平台的文库构建提供了单管解决方案。该协议在单个反应步骤中支持碎片，末端修复和dA拖尾，因此极大地简化了工作流程，缩短了总反应时间和动手时间。

规格：	24Reactions
体积：	0.24 mL
保存：	-25°C至-15°C
随附：	B0330 10X碎片缓冲液 B0340增强剂
检测：	DNA文库构建分析
质量控制分析	通过执行文库构建，PCR / qPCR和测序来评估5X WGS碎裂混合物的功能。
其他：	在组装之前测试酶组分，发现没有污染性内切核酸酶和外切核酸酶。通过SDS-PAGE测定，酶纯度> 95％，通过qPCR确认可忽略的大肠杆菌基因组DNA污染。

部分产品信息：

货号	品名	规格	浓度	品牌
N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

Enzymatics L6060L 说明书

发表于2024年7月5日由上海金畔生物科技有限公司

货号：L6060L

品名：Taq DNA Ligase（Taq DNA连接酶）

产品描述：

使用NAD +作为辅因子，Taq DNA连接酶催化在含有相邻5'-磷酰基和3'-羟基末端的双链DNA中形成磷酸二酯键。

规格：	20,000 U
浓度：	40,000 U/ml
保存：	-25°C至-15°C
纯度（SDS-PAGE）	> 99％
活性：	400,000 U/mg
SS核酸外切酶	400 U <5.0% released
DS核酸外切酶	400 U <1.0% released
DS内切核酸酶	400 U = No conversion
大肠杆菌 DNA污染	400 U <10 copies

蛋白质来源

重组大肠杆菌菌株携带Taq DNA连接酶基因的克隆。

提供：

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
0.1% Tween-20
50% glycerol
pH 7.5 @ 25°C

随附：

B6060 (10X Taq DNA Ligase Buffer)

10X Taq DNA Ligase Buffer (B6060)：
200 mM Tris-HCI
250 mM KCl
100 mM MgCl2
5 mM NAD
0.1% Triton X-100
pH 7.6 @ 25°C

单位定义
1单位定义为在50μl1X Taq DNA连接酶缓冲液中10分钟后加入1μg用Sma I和Sal I切割的Lambda DNA的12个碱基粘性末端的50％所需的Taq DNA连接酶的量在45℃孵育。

质量控制分析

单位表征测定
单位活性使用2倍系列稀释法测量。在1X Taq DNA连接酶反应缓冲液中进行酶批次的稀释并加入到50μL含有λHind III消化的DNA和1X Taq DNA连接酶反应缓冲液的反应中。将反应在45℃孵育10分钟，停止，并在用溴化乙锭染色的0.8％琼脂糖凝胶上分析。

SDS-Page（物理纯度评估）

将2.0μL浓缩的酶溶液加载到变性的4-20％Tris-甘氨酸SDS-PAGE凝胶上，该凝胶侧翼为宽范围的MW标记和2.0μL的1：100稀释的相同酶物种。电泳后，将凝胶染色并比较样品以确定物理纯度。该测试的验收标准要求浓缩样品中污染带的总量不超过稀释样品中目的蛋白质条带的质量，确认浓缩样品的纯度大于99％。

污染测试

单链核酸外切酶活性

将含有10,000cpm放射性标记的单链DNA底物的50μL反应液和10μL在37℃下孵育4小时的酶溶液导致TCA可溶性计数的释放小于5.0％。

双链核酸外切酶活性将含有5,000cpm放射性标记的双链DNA底物和10μL酶溶液的50μl反应液在37℃下温育4小时，导致TCA可溶性计数的释放少于1.0％。

双链核酸内切酶活性将含有0.5μgpENZuC DNA和10μL酶溶液的50μL反应物在37℃下温育4小时，结果通过琼脂糖凝胶电泳测定没有肉眼可察觉地转变为带切口的环状DNA。

大肠杆菌 16S rDNA污染测试
重复5μL酶溶液样品变性，并在TaqMan qPCR测定中筛选污染性大肠杆菌基因组DNA，使用对应于16S rRNA基因座的寡核苷酸引物。测试的验收标准是由3个重复的无模板对照样品的平均值产生的阈值循环计数（C t）。基于无模板对照 Ct值和标准曲线数据之间的相关性，该测定的检测限为<10拷贝基因组/样品。

部分产品信息：

货号	品名	规格	浓度	品牌
N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

Enzymatics L6030-HC-L 说明书

发表于2024年7月1日由上海金畔生物科技有限公司

货号：L6030-HC-L

品名：T4 DNA Ligase (Rapid) （T4 DNA连接酶（快速））

产品描述：

T4 DNA连接酶催化双链DNA或RNA的末端5'磷酸和3'羟基之间形成磷酸二酯键。该酶有效连接钝端和粘端，并修复双链DNA，RNA或DNA / RNA杂合体中的单链缺口（1）。

规格：	600,000 U/ml
浓度：	240,000 U
保存：	-25°C至-15°C
纯度（SDS-PAGE）	> 99％
活性：	300,000 U/mg
SS核酸外切酶	6,000 U< 1.0 % Released
DS核酸外切酶	6,000 U< 1.0 % Released
DS内切核酸酶	6,000 U=No Conversion
大肠杆菌 DNA污染	6,000 U <10 copies

蛋白质来源

携带克隆的T4 DNA连接酶基因的重组大肠杆菌菌株。

提供：

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% glycerol
pH 7.5 @ 25°C

随附：

B1010 (2X Rapid Ligation Buffer)
B6030 (10X T4 DNA Ligase Buffer)

2X Rapid Ligation Buffer (B1010):
132mM Tris-HCI
20 mM MgCl2
2 mM DTT
2 mM ATP
15% PEG 6000
pH 7.6 @ 25°C

10X T4 DNA Ligase Buffer (B6030):
500mM Tris-HCI
100 mM MgCl2
50 mM DTT
10 mM ATP
pH 7.6 @ 25°C

单位定义
1单位定义为在23℃孵育30分钟后，在50μl1X DNA连接酶缓冲液中加入100ng具有粘性末端的DNA片段的50％所需的DNA连接酶的量。

使用说明：

1、反应设置

Amount	Description	Final Concentration
10 uL	2X Rapid Ligation Buffer	1X
X uL	Vector	1-10 ng/uL
X uL	Insert	1-10 ng/uL
1 uL	T4 DNA Ligase (600 U/ uL)	30 U/uL
X uL	Type I Water	N/A
20 uL	Total Volume

2、过程操作

1）将所有上述组分加入干净的反应容器中，通过移液充分混合。

2）25°C孵育10分钟。

3）使用PCR清洁柱立即纯化DNA并洗脱约50μL。

4）– 或 – 在TE或水中立即稀释（至少1:10，但足以使0.1-10ng连接产物转化）

5）将0.1-10ng连接产物转化为与载体相容的化学或电子感受态细胞系

3、其他

在1X T4 DNA连接酶反应缓冲液中用Lambda-Hind III DNA片段底物测量，一个酶酶T4 DNA连接酶粘性末端单位相当于约3个粘性末端单位。

一个Weiss单位大约相当于22个Enzymatics粘性末端单位。

T4 DNA连接酶是ATP依赖性的。建议在-20°C储存一年后丢弃反应缓冲液，并用新鲜缓冲液代替以确保*性能。当靶向插入片段：载体比率在2和6之间时，单插入连接是*的。高于6：1的比率将促进插入多个片段，而降至2：1以下将降低连接效率。

对于有问题的连接或DNA浓度未知的情况，可能需要改变比率并进行多次连接。

高浓度PEG的存在将显着降低电感受态细胞的转化效率。为了zui大限度地转化为电感受态细胞，建议采用以下方法：

*：连接后，使用DNA纯化离心柱纯化产物并在50μLTE中洗脱。DNA现在已准备好转化。要转化的DNA的zui终数量应该在0.1-10ng范围内。

更好：在ddH2O或TE中稀释连接产物以降低PEG浓度。要转化的DNA的zui终数量应该在0.1-10ng范围内。

Enzymatics的高浓度T4 DNA连接酶与2X快速连接缓冲液结合使用，极大地刺激了平端连接的速度和效率，因此不建议长时间孵育（> 10分钟），并可能大大降低连接产物的转化效率。为了使正确的插入/载体组合的转化效率zui大化，推荐使用以下方案。

酶学10X T4 DNA连接酶缓冲液不含PEG，并且与标准连接方案相兼容，该方案不规定使用快速/快速/快速格式缓冲液。

部分产品信息：

货号	品名	规格	浓度	品牌
N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

Enzymatics 中国代理

发表于2024年4月4日由上海金畔生物科技有限公司

世界*实验材料供应商Enzymatics上海金畔生物为其中国代理，Enzymatics在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， Enzymatics 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Enzymatics中国代理，Enzymatics上海代理，Enzymatics北京代理，Enzymatics广东代理，Enzymatics江苏代理，Enzymatics湖北代理,Enzymatics天津,Enzymatics黑龙江代理,Enzymatics内蒙古代理,Enzymatics吉林代理,Enzymatics福建代理,Enzymatics江苏代理,Enzymatics浙江代理,Enzymatics四川代理,

Enzymatics公司建立于2006年，专门从事开发、制造和分销酶产品，致力于酶产品研发，为市场带来了特殊的、高品质的产品。 Enzymatics公司建立了严格完整的操作体系，通过了ISO13485:2003和9001:2000体系标准认证，并符合FDA 21 CFR.820质量系统法规中对于常规用途试剂的生产规范。同时Enzymatics的生产能力被许多FDA认证的医疗设备制造商以及的ISO认证机构TÜV SÜD 认可。

Enzymatics 严格质检流程

Enzymatics致力于向客户提供*品质产品。因此，Enzymatics不仅使用制造技术生产产品，同时进行zui严格的质量检验以保证产品的品质。Enzymatics技术的质量监控检测类型包括：

■ 检测核酸酶污染：使用灵敏度高于同类厂家1000倍以上的检测方法

■ SDS‐PAGE检测：使用硝酸银染色，灵敏度高于使用考染方法50倍以上

■ 残留DNA污染：使用qPCR扩增16s rRNA亚基

T4 DNA连接酶：连接率高于其他产品20%-30%；提供高浓度/低浓度产品供选择
T7 DNA连接酶：连接效率高于传统T4 DNA连接酶
T3 DNA连接酶：连接效率高于传统T4 DNA连接酶，适用于高盐度/高pH值缓冲液
VeraSeq DNA Polymerase 2.0 ：超高保真度、聚合速度快、适用于高GC含量样本扩增的新型高保真聚合酶
VeraSeq ULtra DNA Polymerase：高保真度、聚合速度快、适用于重亚硫酸盐测序和高GC含量样本扩增的新型聚合酶
Phoenix Hot Start Taq DNA Polymerase：抗体介导的热启动酶，适用于高GC含量样本扩增
Taq DSC 2.0 DNA Polymerase ：高性能的新型热启动酶
Omni klentaq：可直接从血液、土壤等样本中扩增目的片段，无需核酸提取
Taq-B DNA Polymerase：高品质Taq酶，高性价比

明星产品推荐： Enzymatics连接酶系列

Enzymatics为您提供高连接效率的系列连接酶产品，包括DNA连接酶与RNA连接酶，具有高纯度、高连接率等特点。其T4连接酶、T3连接酶以及T7连接酶在多篇文献中均有提及，超高的连接效率是您的*选择。使用Enzymatics提供的连接酶，连接反应中遇到的问题必将迎刃而解。

连接效率比较

粘性末端：T7≥T3>T4

平末端(添加15% PEG 6000)： T3≈T7>T4

比较结果来源于文献：Characterization of Bacteriophage T3 DNA Ligase. Liang Cai. J. Biochem. 135, 397–403 (2004)

明星产品推荐： Enzymatics PCR聚合酶系列

Enzymatics销售多种PCR聚合酶产品，包括高保真聚合酶、热启动聚合酶、常规聚合酶以及无需核酸提取，可直接扩增目标片段的血源直扩聚合酶，满足您的不同研究需求。

Omni Klentaq 新一代血源直扩热启动PCR 酶

Omni Klentaq是经工程改造过的Taq DNA聚合酶，缺乏5‘→3’ 和3‘→5’ 外切酶结构域，能够耐受全血、土壤样本中存在的抑制剂，能够直接扩增全血样品中的 DNA 而无需事先提纯。

·高耐受度：能够耐受高达 40% 的全血；同样可耐受土壤、食品中的抑制剂及插入染料；

·样本直扩：无需核酸提取即可直接从样本中扩增片段；

·热启动：降低引物二聚体产生的风险；

VeraSeq DNA Polymerase 2.0 超高保真度与高速扩增的结合

VeraSeq DNA Polymerase 2.0是经工程改造过的Taq DNA聚合酶，具有校正功能。VeraSeq 2.0扩增速度极快，同时具有*的保真度。同时具备了率、高保真性、等特点，是您PCR产品的*选择。

高保真性：保真度高于普通Taq酶50倍以上；

高速扩增：扩增速度高达1kb/15秒；

配有适合于扩增高GC含量样本的缓冲液；

可扩增长达8kb的片段

VeraSeq ULtra DNA Polymerase 高保真度与高速扩增的结合
VeraSeq ULtra DNA Polymerase是经工程改造过的Taq DNA聚合酶，具有校正功能。 VeraSeq ULtra扩增速度极快，保真度高，可用于重亚硫酸盐测序。

高保真性：保真度高于普通Taq酶25倍以上；

高速扩增：扩增速度高达1kb/15秒；

配有适合于扩增高GC含量样本的缓冲液；

适用于重亚硫酸盐测序；

可扩增长达8kb的片段

Taq-B DNA Polymerase 标准PCR Taq酶
Taq-B DNA 聚合酶是耐热性的DNA聚合酶，提供优化过的Buffer体系，适用于科研和工业生产。

经优化的Buffer 体系

高标准质检过程，保证质量

高性价比

Taq DSC 2.0 DNA Polymerase 热启动PCR Taq酶

Enzymatics推出的DSC 2.0热启动聚合酶使用经修饰的多聚核苷酸，能与聚合酶结合，在室温条件下阻断聚合酶与引物等组分结合，从而预防了引物二聚体出现。

新型热启动原理

经优化过的Buffer体系

部分产品列表

货号	品名	规格	浓度	品牌
N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

enzymatics代理

发表于2024年4月4日由上海金畔生物科技有限公司

enzymatics代理

简要描述：enzymatics代理，enzymatics上海代理，enzymatics北京代理，enzymatics 代理，enzymatics一级代理，enzymatics试剂盒，enzymatics kit，enzymatics国内代理

上海金畔生物科技有限公司enzymatics专业代理，具体产品信息欢迎电询：021-50837765

详细介绍

产品咨询

enzymatics代理，enzymatics上海代理，enzymatics北京代理，enzymatics 代理，enzymatics一级代理，enzymatics试剂盒，enzymatics kit，enzymatics国内代理

上海金畔生物科技有限公司enzymatics专业代理，具体产品信息欢迎电询：

Enzymatics 严格质检流程
Enzymatics致力于向客户提供*品质产品。因此，Enzymatics不仅使用制造技术生产产品，同时进行zui严格的质量检验以保证产品的品质。Enzymatics技术的质量监控检测类型包括：
■ 检测核酸酶污染：使用灵敏度高于同类厂家1000倍以上的检测方法
■ SDS‐PAGE检测：使用硝酸银染色，灵敏度高于使用考染方法50倍以上
■ 残留DNA污染：使用qPCR扩增16s rRNA亚基

Poly(A) Polymerase	P7460L	1,000 U	5,000 U/mL
RecA	Y9260L	1.5 mg	2.0 mg/mL
RNAse H	Y9220L	5,000 U	5,000 U/ml


	Y9220F	770 U	5,000 U/ml
RNAse Inhibitor	Y9240L	20,000 U	40,000 U/ml
SPARK™ DNA Sample Prep Kit for Illumina®	SPK0001-V08	N/A	N/A
SPARK™ DNA Sample Prep Kit Ion Torrent™ Platform	SPK0002-V08	N/A	N/A
T3 DNA Ligase	L6010L	900,000 U	3,000,000 U/ml


	L6010F	140,000 U	3,000,000 U/ml
T4 DNA Ligase	L6030-LC-L	150,000 U	120,000 U/ml
T4 DNA Ligase (Rapid)	L6030-HC-L	240,000 U	600,000 U/ml
T4 DNA Polymerase	P7080L	2,000 U	3,000 U/ml
T4 Gene 32 Protein	Y9130L	1.0 mg	10 mg/mL
T4 Polynucleotide Kinase	Y9040L	10,000 U	10,000 U/ml
T4 RNA Ligase 1	L6050L	10,000 U	20,000 U/ml


	L6050F	1,540 U	20,000 U/ml
T4 RNA Ligase 2	L6080L	4,500 U	30,000 U/ml
T4 RNA Ligase 2 Truncated	L6070L	500 U	5,000 U/ml
T7 DNA Ligase	L6020L	900,000 U	3,000,000 U/ml


	L6020F	138,000 U	3,000,000 U/ml
T7 DNA Polymerase	P7260L	3,500 U	10,000 U/ml


	P7260F	550 U	10,000 U/ml
T7 RNA Polymerase	P7180L	50,000 U	50,000 U/ml


	P7180F	7,700 U	50,000 U/ml
Taq DNA Ligase	L6060L	20,000 U	40,000 U/ml


	L6060F	3,100 U	40,000 U/ml
Taq DSC 2.0 DNA Polymerase	P7110L	1,250 U	5,000 U/ml


	P7110F	200 U	5,000 U/ml
Taq-B DNA Polymerase	P7250L	10,000 U	5,000 U/ml


	P7250F	1,540 U	5,000 U/ml
Terminal deoxynucleotidyl Transferase (TdT)	P7070L	6,000 U	20,000 U/ml
Thermolabile UDG	G5020L	500 U	1,000 U/ml
Uracil DNA Glycosylase (UDG)	G5010L	10,000 U	2,000 U/ml


	G5010F	1,540 U	2,000 U/ml
VeraSeq™ 2.0 High-Fidelity DNA Polymerase	P7511L	500 U	2,000 U/ml


	P7511S	100 U	2,000 U/ml
VeraSeq™ ULtra DNA Polymerase	P7520L	500 U	2,000 U/ml


	P7520S	100 U	2,000 U/ml
φ29 DNA Polymerase (High Concentration)	P7020-HC-L	2,000 U	100,000 U/ml
φ29 DNA Polymerase (Low Concentration)	P7020-LC-L	2,000 U	10,000 U/ml


	P7020-LC-F	310 U	10,000 U/ml

上海金畔生物科技有限公司
：

*8006

:
:http://www.jinpanbio.com/

Enzymatics P7050L 说明书

发表于2024年3月3日由上海金畔生物科技有限公司

货号：P7050L

品名：DNA Polymerase I（DNA聚合酶I）

产品描述：

DNA聚合酶I是一种嗜温性DNA聚合酶，除3'→5'和5'→3'核酸外切酶活性外，还具有5'-3'DNA合成。DNA合成和5'→3'核酸酶特征的组合使DNA合成期间的缺口翻译成为可能。

规格：	5,000 U
浓度：	10,000 U/ml
纯度（SDS-PAGE）	> 99％
活性：	6,850 U/mg
SS核酸外切酶	实用
DS核酸外切酶	实用
DS内切核酸酶	200 U = no conversion
大肠杆菌 DNA污染	200 U <10 copies

蛋白质来源

重组大肠杆菌菌株携带的POLA基因。

提供：

25 mM Tris-HCl
0.1 mM EDTA
1.0 mM DTT
50% glycerol
pH 7.4 @ 25°C

随附：

pB0110 (10X Blue Buffer)

10X Blue Buffer (B0110)：
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
10 mM DTT
pH 7.9 @ 25°C

单位定义
1单位定义为在37℃30分钟内将10nmol dNTPs转化为酸不溶性物质所需的聚合酶量。

质量控制分析

单位表征测定
单位活性使用2倍系列稀释法测量。在1X Blue反应缓冲液中制备酶的稀释液，并加入含有小牛胸腺DNA，1X蓝色反应缓冲液，3H-dTTP和100μMdNTP的50μL反应物中。将反应在37℃孵育10分钟，在冰上沉降，并使用TCA沉淀法分析。

蛋白质浓度（OD 280）测量使用使用2.0mg / ml BSA样品（Pierce Cat＃23209）标准化的Nanodrop ND-1000分光光度计

在OD 280处分析2.0μL酶样品并用产品储存溶液冲切。使用80,790的消光系数和103,118道尔顿的分子量将观察到的3个重复样品的平均测量值转换成mg / mL。

SDS-Page（物理纯度评估）
将2.0μL浓缩的酶溶液加载到变性的4-20％Tris-甘氨酸SDS-PAGE凝胶上，该凝胶侧翼为宽范围的MW标记和2.0μL的样品的1:100稀释物。电泳后，将凝胶染色并比较样品以确定物理纯度。该测试的验收标准要求浓缩样品中污染带的总量不超过稀释样品中目的蛋白质条带的质量，确认浓缩样品的纯度大于99％。

污染测试

单链核酸外切酶活性

将含有10,000cpm放射性标记的单链DNA底物和10μL酶溶液的50μL反应物在37℃下孵育4小时，导致TCA可溶性计数的释放超过80％。

双链核酸外切酶活性将含有5,000cpm放射性标记的双链DNA底物的50μL反应液和10μL在37℃下孵育4小时的酶溶液导致超过50％的TCA可溶性计数的释放。

双链核酸内切酶活性将含有0.5μgpBR322 DNA和10μL酶溶液的50μL反应物在37℃下温育4小时，结果通过琼脂糖凝胶电泳确定没有肉眼可察觉地转化为带切口的环状DNA。

大肠杆菌 16S rDNA污染测试
重复5μL酶溶液样品变性，并在TaqMan qPCR测定中筛选污染性大肠杆菌基因组DNA，使用对应于16S rRNA基因座的寡核苷酸引物。测试的验收标准是由3个重复的无模板对照样品的平均值产生的阈值循环计数（C t）。基于无模板对照 Ct 值与标准曲线数据之间的相关性，该测定的检测限为<10拷贝基因组/样品。

部分产品信息：

货号	品名	规格	浓度	品牌
N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

Enzymatics N2010L说明书

发表于2024年3月3日由上海金畔生物科技有限公司

货号：N2010L

品名：100 mM 75µmol dNTP Solution Set

产品描述：

一组四种天然脱氧核苷酸的浓缩100mM溶液。

规格：	75 umol
浓度：	100 mM
保存：	-25°C至-15°C
纯度	>99.9％
碱基纯度	99.5%
焦磷酸	0.003 pmol/pmol of nucleotide
ATP	未检测到
Nick 活性	未检测到
DNase活性	未检测到
RNase活性	未检测到

提供：
pH7.5, 各100mM的dNTP（dATP，dCTP，dGTP，dTTP）

部分产品信息：

货号	品名	规格	浓度	品牌
N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

Enzymatics L6030-W-L 说明书

发表于2024年3月3日由上海金畔生物科技有限公司

货号：L6030-W-L

品名：WGS Ligase （WGS连接酶）

产品描述：

WGS连接酶针对WGS碎片化后的连接进行了优化。它催化在双链DNA或RNA的末端5'磷酸和3'羟基之间形成磷酸二酯键。该酶有效连接平端和粘性末端，并修复双链RNA，RNA或DNA / RNA杂合体中的单链缺口（1）。

规格：	24 Reactions
浓度：	N/A
体积：	0.24 mL
保存：	-25°C至-15°C
纯度（SDS-PAGE）	> 99％
活性：	300,000 U/mg
SS核酸外切酶	6,000 U< 1.0 % Released
DS核酸外切酶	6,000 U< 1.0 % Released
DS内切核酸酶	6,000 U=No Conversion
大肠杆菌 DNA污染	6,000 U <10 copies

蛋白质来源

携带克隆的T4 DNA连接酶基因的重组大肠杆菌。

提供：

10 mM Tris-HCl
50 mM KCl
1 mM dithiothreitol
0.1 mM EDTA
50% glycerol
pH 7.5 @ 25°C

随附：

B9020: 330mM Tris-HCl
50mM MgCl
5mM DTT
5mM ATP
30% PEG 6000
pH 7.6 @ 25°C

单位定义
1单位定义为在23℃孵育30分钟后，在50μl1X DNA连接酶缓冲液中加入100ng具有粘性末端的DNA的片段的50％所需的DNA连接酶的量。

分子量

55,292Da

质量控制分析

单位表征测定
单位活动使用2倍连续稀释法测量。在1X DNA连接酶反应缓冲液中进行酶批次的稀释并加入到20μL含有双链DNA的片段和1X DNA连接酶反应缓冲液的反应中。将反应在23℃孵育30分钟，停止，并在用溴化乙锭染色的1％琼脂糖凝胶上分析。

蛋白质浓度由OD 280吸光度确定。

物理纯度通过浓缩和稀释的酶溶液的SDS-PAGE评估，然后进行银染检测。通过比较浓缩样品中污染物条带的总质量与稀释样品中目的蛋白质条带的质量来评估纯度。

单链核酸外切酶在含有放射性标记的单链DNA底物的50μL反应液中和10μL酶溶液在37℃下温育4小时。

在含有放射性标记的双链DNA底物的50μl反应液中和在37℃下温育4小时的10μL酶溶液测定双链核酸外切酶。

双链核酸内切酶在含有0.5μg质粒DNA的50μL反应液和10μL酶溶液中在37℃下温育4小时。

大肠杆菌的16S rDNA污染用5μL评价 ř的变性，并筛选酶溶液在的TaqMan qPCR测定对于污染的存在eplicate样品大肠杆菌使用对应于所述的16S rRNA基因座寡核苷酸引物的基因组DNA。

部分产品信息：

货号	品名	规格	浓度	品牌
N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

Enzymatics Y9040L 说明书

发表于2024年3月2日由上海金畔生物科技有限公司

货号：Y9040L

品名：T4 Polynucleotide Kinase（T4多核苷酸激酶）

产品描述：

T4多核苷酸激酶（PNK）催化ATP的末端γ位磷酸酯转移和交换到双链和单链DNA，RNA和核苷3'-单磷酸酯分子的5'-羟基末端（1）。T4 PNK还表现出3'-磷酸酶和2'，3'环磷酸二酯酶活性（2,3,4,5,6）。

规格：	10,000 U
浓度：	10,000 U/ml
保存：	-25°C至-15°C
纯度（SDS-PAGE）	> 99％
活性：	133,333U / mg
SS核酸外切酶	2000 U <5.0% released
DS核酸外切酶	2000 U <1.0% released
DS内切核酸酶	2000 U = No conversion
大肠杆菌 DNA污染	2000 U <10 copies

蛋白质来源

从表达重组T4多核苷酸激酶基因的大肠杆菌中纯化而得。

补充：

10 mM Tris-HCl

50 mM KCl

0.1 碌M ATP

1.0 mM DTT

0.1 mM EDTA

50% glycerol

pH 7.4 @ 25掳C

随附：

B9040 (10X T4 Polynucleotide Kinase Buffer)：

10X多核苷酸激酶缓冲液（B9040）700mM

Tris-HCl

100mM MgCl 2

50mM DTT

pH 7.6在25℃

单位定义
一个单位定义为在1X T4多核苷酸激酶反应缓冲液中，37℃下30分钟内催化掺入1 nmol [ 32 P]（ATP供体）的酶量。

质量控制分析

单位表征测定
单位活性使用2倍系列稀释法测量。在1X反应缓冲液中制备酶的稀释液，并将其加入到50μL含有10μMOligo dT单链DNA，1X PNK反应缓冲液和66μMATP的反应中，并将[γ- 32 P] ATP反应在37°孵育30分钟将其置于冰上，并使用Sambrook和Russell的方法（Molecular Cloning，v3,2001，pp.A8.25-A8.26）进行分析。

蛋白质浓度（OD 280）测量使用使用2.0mg / ml BSA样品（Pierce Cat＃23209）标准化的Nanodrop ND-1000分光光度计
在OD 280处分析2.0μL酶样品并用产品储存溶液冲切。使用消光系数63,440和分子量34,620道尔顿将观察到的3个重复样品的平均测量结果转换为mg / mL。

SDS-Page（物理纯度评估）
将2.0μL酶溶液加载到变性的4-20％Tris-甘氨酸SDS-PAGE凝胶上，其侧翼为宽范围MW标记和2.0μL1：100稀释的样品。电泳后，将凝胶染色并比较样品以确定物理纯度。该测试的验收标准要求浓缩样品中污染带的总量不超过稀释样品中目的蛋白质条带的质量，确认浓缩样品的纯度大于99％。

部分产品信息：

货号	品名	规格	浓度	品牌
N2070-10-L	ATP Solution	50 µmol	10 mM	Enzymatics
N2050-10-L	10 mM dNTP Solution Mix	100 µmol	10 mM	Enzymatics
N2010L	100 mM 75µmol dNTP Solution Set	75 µmol	100 mM	Enzymatics
N2010-A-L	100 mM dATP Solution	75 µmol	100 mM	Enzymatics
N2050L	100µmol dNTP Solution Mix	100 µmol	25 mM	Enzymatics
Y9180L	10x Uracil Cleavage System	750 Reactions	N/A	Enzymatics
P7670	2x HiFi PCR Master Mix	1 rxn	2x	Enzymatics
Y9420L	5x ER/A-Tailing Enzyme Mix	24 reactions	N/A	Enzymatics
Y9380L	E. coli Pyrophosphatase	50 U	100 U/mL	Enzymatics
Y9030L	E. coli Single-Stranded DNA Binding Protein	1.5 mg	2.75 mg/ml	Enzymatics
Y9140-HC-L	End-Repair Mix (High Concentration)	75 Reactions	1 Rxn/µL	Enzymatics
Y9140-LC-L	End-Repair Mix (Low Concentration)	200 Reactions	1 Rxn/µL	Enzymatics
Y9080L	Endonuclease VIII	10,000 U	10,000 U/ml	Enzymatics
P7600L	EnzScript™ (MMLV Reverse Transcriptase RNase H-)	10,000 U	200,000 U/mL	Enzymatics
X8010L	Exonuclease I	30,000 U	20,000 U/ml	Enzymatics
X8020L	Exonuclease III	50,000 U	100,000 U/ml	Enzymatics
P7010-HC-L	Klenow (3′→ 5′ exo-) (High Concentration)	10,000 U	50,000 U/ml	Enzymatics
P7010-LC-L	Klenow (3′→ 5′ exo-) (Low Concentration)	10,000 U	5,000 U/ml	Enzymatics
P7060L	Klenow Fragment	2,500 U	5,000 U/ml	Enzymatics
X8030L	Lambda Exonuclease	10,000 U	5,000 U/ml	Enzymatics
P7040L	M-MuLV Reverse Transcriptase	100,000 U	200,000 U/ml	Enzymatics
P7090L	Mako DNA Polymerase (3′→ 5′ exo-)	3,000 U	30,000 U/ml	Enzymatics
P7140-HC-L	Manta 1.0 DNA Polymerase (High Concentration)	100,000 U	400,000 U/ml	Enzymatics
P7140-LC-L	Manta 1.0 DNA Polymerase (Low Concentration)	100,000 U	40,000 U/ml	Enzymatics
P7610L	NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions	250 Reactions	2X	Enzymatics
P7590L	Phoenix™ Hot Start Taq DNA Polymerase	500 U	5,000 U/ml	Enzymatics
P7460L	Poly(A) Polymerase	1,000 U	5,000 U/mL	Enzymatics
Y9260L	RecA	1.5 mg	2.0 mg/mL	Enzymatics
Y9220L	RNAse H	5,000 U	5,000 U/ml	Enzymatics
Y9240L	RNAse Inhibitor	20,000 U	40,000 U/ml	Enzymatics
L6010L	T3 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
L6030-LC-L	T4 DNA Ligase	150,000 U	120,000 U/ml	Enzymatics
L6030-HC-L	T4 DNA Ligase (Rapid)	240,000 U	600,000 U/ml	Enzymatics
P7080L	T4 DNA Polymerase	2,000 U	3,000 U/ml	Enzymatics
Y9130L	T4 Gene 32 Protein	1.0 mg	10 mg/mL	Enzymatics
Y9040L	T4 Polynucleotide Kinase	10,000 U	10,000 U/ml	Enzymatics
L6050L	T4 RNA Ligase 1	10,000 U	20,000 U/ml	Enzymatics
L6080L	T4 RNA Ligase 2	4,500 U	30,000 U/ml	Enzymatics
L6070L	T4 RNA Ligase 2 Truncated	500 U	5,000 U/ml	Enzymatics
L6020L	T7 DNA Ligase	900,000 U	3,000,000 U/ml	Enzymatics
P7260L	T7 DNA Polymerase	3,500 U	10,000 U/ml	Enzymatics
P7180L	T7 RNA Polymerase	50,000 U	50,000 U/ml	Enzymatics
L6060L	Taq DNA Ligase	20,000 U	40,000 U/ml	Enzymatics
P7110L	Taq DSC 2.0 DNA Polymerase	1,250 U	5,000 U/ml	Enzymatics
P7250L	Taq-B DNA Polymerase	10,000 U	5,000 U/ml	Enzymatics
P7620L	TaqIT	5,000 U	50,000 U/mL	Enzymatics
P7070L	Terminal deoxynucleotidyl Transferase (TdT)	6,000 U	20,000 U/ml	Enzymatics
G5020L	Thermolabile UDG	500 U	1,000 U/ml	Enzymatics
Y9370L	Thermostable Pyrophosphatase	1,250 U	2,000 U/ml	Enzymatics
G5010L	Uracil DNA Glycosylase	10,000 U	2,000 U/ml	Enzymatics
P7511L	VeraSeq™ 2.0 High-Fidelity DNA Polymerase	500 U	2,000 U/ml	Enzymatics
P7520L	VeraSeq™ ULtra DNA Polymerase	500 U	2,000 U/ml	Enzymatics
L6030-W-L	WGS Ligase	24 Reactions	N/A	Enzymatics
P7640L	ZipScript™ One-Step RT-qPCR Kit	1000 Reactions	N/A	Enzymatics
P7020-HC-L	φ29 DNA Polymerase (High Concentration)	2,000 U	100,000 U/ml	Enzymatics
P7020-LC-L	φ29 DNA Polymerase (Low Concentration)	2,000 U	10,000 U/ml	Enzymatics

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标签归档：enzymatics

Enzymatics N2050-10-L说明书

品名：10 mM dNTP Solution Mix

Enzymatics Y9410L 产品说明书

品名：5X WGS Fragmentation Mix（5X WGS碎片混合物）

Enzymatics L6060L 说明书

品名：Taq DNA Ligase（Taq DNA连接酶）

Enzymatics L6030-HC-L 说明书

品名：T4 DNA Ligase (Rapid) （T4 DNA连接酶（快速））

Enzymatics 中国代理

enzymatics代理

enzymatics代理

Enzymatics P7050L 说明书

品名：DNA Polymerase I（DNA聚合酶I）

Enzymatics N2010L说明书

品名：100 mM 75µmol dNTP Solution Set

Enzymatics L6030-W-L 说明书

品名：WGS Ligase （WGS连接酶）

Enzymatics Y9040L 说明书