cellecta EF1-Bleo说明书

cellecta EF1-Bleo说明书

 

具有CMV启动子的cDNA克隆载体(CMV-MCS-EF1选择)

$ 700.00

选带盒– SELECT ONE:EF1-布莱EF1-爆炸EF1-NeoEF1-PuroEF1-RFPEF1-RFP-Puro

尺寸10微克

储存温度| 交货时间-20℃| 1天

 EF1-Bleo / 10 ug / -20C | 1 day EF1-Blast / 10 ug / -20C | 1 day EF1-Neo / 10 ug / -20C | 1 day EF1-Puro / 10 ug / -20C | 1 day EF1-RFP / 10 ug / -20C | 1 day EF1-RFP-Puro / 10 ug / -20C | 1 day

产品信息

慢病毒载体,用于从CMV启动子克隆和表达cDNA。选择标记是从单独的EF1启动子表达的。

Nordicbiosite EKX-2EF538说明书


Nordicbiosite EKX-2EF538说明书

Cytokeratin 18

文章编号

EKX-2EF538

产品类别

分析和检测

应用

ELISA

尺寸

96T

BioSite品牌

BioSite ELISA

描述

人细胞角蛋白18-M30 ELISA试剂盒

检测方法

夹心ELISA,双抗体

供应商

BIOSITE

别名

M30

范围

15.625-1000mIU / ml的

灵敏度

9.375mIU / ml的

物种反应性

人的

存储

4℃,6个月

 

 

 

货号

品名

规格

目录价

品牌

ABE-001783

IL-13 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-006FA6

IL-6 (Rat) ELISA Kit

96T

14200

nordicbiosite

ABE-00D9B0

Human Cyclophilin A ELISA Kit

96T

14200

nordicbiosite

ABE-010C24

Albumin (Serum) (wide range) (Human) ELISA Kit (480T)

5 x 96T

30800

nordicbiosite

ABE-01C807

Leptin (Human) ELISA Kit (960T)

10 x 96T

65000

nordicbiosite

ABE-01F731

Asprosin (Human) ELISA Kit

96T

20400

nordicbiosite

ABE-01FC42

ASGPR1 (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-0214AD

CTRP6 (Human) ELISA Kit

96T

17200

nordicbiosite

ABE-021916

Regerating Islet-derived 1 beta (REG1B) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-03-01

PCSK9 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-03-02

PCSK9 (Rat) ELISA Kit

96T

14200

nordicbiosite

ABE-03093E

Soluble SCF (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-03EE5D

IL-5 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-047D47

Stathmin1/Metablastin (Human) ELISA Kit

96T

17200

nordicbiosite

ABE-05765D

Amyloid Precursor-Like Protein 1 (APLP1)(Soluble)(Human) ELISA Kit (192T)

2 x 96T

29600

nordicbiosite

ABE-05FF37

Angiotensinogen (AGT)/Serpin A8 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-06-01

Soluble LOX-1 (Human) ELISA Kit

96T

11200

nordicbiosite

ABE-0610C5

Osteonectin/SPARC (Rat) ELISA Kit

96T

14200

nordicbiosite

ABE-0704BE

GDF-15 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-073FEA

Eosinophil Cationic Protein (ECP) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-07B560

Meteorin Like (METRNL)/Subfatin (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-07E708

CTRP-13 (Human, Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-0817DC

APP (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-08D1FC

Streptavidin-HRP Conjugate (High sensitive, 8000-20000x)

50µL

2000

nordicbiosite

ABE-095-01

Proinsulin (Human) ELISA Kit

96T

11200

nordicbiosite

ABE-09CE64

Mouse Soluble ST2/IL-1R4ELISA Kit

96T

14200

nordicbiosite

ABE-09E264

Interleukin-13 Receptor alpha 1 (IL013RA1) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-0A0A59

BDNF (Human, Mouse, Rat) Ultrasensitive ELISA Kit

96T

13800

nordicbiosite

ABE-0C7DB1

LYVE-1 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-0CA14C

CTRP-5 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-0E1053

CXCL-14/BRAK (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-0E5216

GASP-2 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-0E7069

CTRP-9 (Human) ELISA Kit

96T

13800

nordicbiosite

ABE-0F4BD8

ACVR IIB (Soluble) (Human) ELISA KIT

96T

15200

nordicbiosite

ABE-0F52D3

SPARC (Human) ELISA Kit

96T

11200

nordicbiosite

ABE-0FE7C9

Dilution Buffer (Ready to use)

60mL

1400

nordicbiosite

ABE-100-02

Resisitin (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-10-01

Adiponectin (Total) (Human) ELISA KIT

96T

13800

nordicbiosite

ABE-10-06

Adiponectin (Rat/Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-107-05

RBP4 (Rat/Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-10A7E5

IL-1F9/IL-36G (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-10C28A

Asprosin (Human) ELISA Kit

96T

20400

nordicbiosite

ABE-10C5CF

IL-33 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-1101D8

Osteonectin/SPARC (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-112-01

sRAGE (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-112-03

sRAGE (Rat/Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-112C37

Fetuin A (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-1162ED

CNPY2 (Human) ELISA Kit

2 x 96T

30200

nordicbiosite

ABE-11BBF5

Fetuin A (Human) ELISA Kit (960T)

10 x 96T

78800

nordicbiosite

ABE-11C0D0

SPARCL1 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-121-02

PBEF1/Visfatin (Rat) ELISA Kit

96T

14200

nordicbiosite

ABE-123-02

VEGF R2 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-124-02

VEGF-R3 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-120D

Arginase 1 (ARG1) (Human) ELISA Set

1960T

34800

nordicbiosite

ABE-125ECC

TSLP (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-12A82C

NCAM-1/CD56 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-12BB1A

RANK Ligand (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-13165B

Annexin V-FITC Apoptosis Assay Kit

200 Test

11600

nordicbiosite

ABE-13AF38

Alpha Fetoprotein (AFP) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-141-01

Paraoxonase 1 (PON1) (Serum) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-145-01

FGF-21 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-145-03

FGF-21 (Rat/Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-145-08

FGF-21 (Mouse/Rat)) ELISA Kit

96T

14200

nordicbiosite

ABE-1463FD

CTRP-9(Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-148-01

FGF-19 (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-151-01

EG-VEGF/PROK1 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-1529AE

Vitamin D Binding Protein (VDBP) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-155-01

GP130 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-160557

CNPY2 (Human) ELISA Kit

5 x 96T

61000

nordicbiosite

ABE-16AD76

Azurocidin/CAP37 (Human) ELISA Kit (480T)

480T

58800

nordicbiosite

ABE-16E2A0

Delta Like Protein 4 (DLL4) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-174AB0

NRG 1-beta 1 (Human) ELISA Kit

96T

18200

nordicbiosite

ABE-175525

DKK1 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-176C8E

ESM1 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-186-01

KIM-1/TIM-1 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-1860D4

Nidogen-1/Entactin (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-1948B3

RBP4 (Active) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-196-02

CD36 (Soluble) (Human) ELISA Kit (for serum and plasma)

96T

14200

nordicbiosite

ABE-196-03

CD36 (Soluble) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-196C45

Galectin-3 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-197-01

Galectin-7 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-199-01

Galectin-3 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-19D2B2

MMP10 (Total)(Human) ELISA Kit

96T

13000

nordicbiosite

ABE-1A7D0C

Omentin 1 (Human) ELISA Kit

96T

22200

nordicbiosite

ABE-1A86D0

RNase A/RNASE 1 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-1AC2F6

Mouse PCSK9 ELISA Kit

96T

14200

nordicbiosite

ABE-1AD6E8

IL-33 (Human) ELISA Set

480T

19800

nordicbiosite

ABE-1B5348

sRAGE (Human) ELISA Development Kit

SET

19200

nordicbiosite

ABE-1B7D88

Neurotrophin 3 (NT-3) ELISA Kit

96T

13000

nordicbiosite

ABE-1BAF53

sTRAIL R4/DcR2/CD264 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-1BC686

CD21 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-1BD6C9

NT-4 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-1C4907

Chemokine CCL17/TRAC (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-1C676C

Resisitin (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-1C79

TGF-alpha 3 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-1F6623

Sample Interaction Reduce Solution (50 µL per well)

6mL

1200

nordicbiosite

ABE-1FBC5D

Cerebellin-1 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-1FBDBD

SDF-1/CXCL-12 (Human) ELISA Development Set

SET

19800

nordicbiosite

ABE-1FE705

Neprilysin/CD10 (Mouse) ELISA Kit

96T

15200

nordicbiosite

ABE-20159E

LAMp-3/CD1208 (Soluble)(Human) ELISA Kit

96T

14200

nordicbiosite

ABE-209E9F

PF4 (Human) ELISA Kit

96T

11200

nordicbiosite

ABE-20E4A0

FDC-SP (Human) ELISA Kit

5 x 96T

59000

nordicbiosite

ABE-212DC8

PLGF/PGF (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-213-06

FABP-3/HFABP (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-214A3E

CD66/CEACAM1 (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-219A67

IL-1 beta (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-21CEFC

IL-1RN (Secreted) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-21DDA0

PBEF1/Visfatin (Human) ELISA Kit (192T)

2 x 96T

24600

nordicbiosite

ABE-21F497

Surfactant Protein D (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-220-01

MCP-1/CCL-2 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-2203E9

Lipocalin-2/NGAL (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-222408

S100A8 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-2266BA

ATPase Inhibitor Factor 1 (ATPIF1) (Soluble Mitochondrial) (Human) ELISA Kit

96T

16000

nordicbiosite

ABE-22B1D1

Secretagogin (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-233-01

Lipocalin-2/NGAL (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-233-06

Lipocalin-2/NGAL (Rat/Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-2383AD

IL-18 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-2428E7

Soluble ACVRL1/ALK-1 (Human) ELISA Kit

96T

14600

nordicbiosite

ABE-24B150

CNTF (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-250-03

Soluble ICAM-1/CD54(Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-250-04

Soluble ICAM-1/CD54 (Rat) ELISA Kit

96T

14200

nordicbiosite

ABE-257912

FSTL3 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-25FB3F

IL-12 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-266C6D

Cystatin C (Human) ELISA Kit (960T)

10 x 96T

79600

nordicbiosite

ABE-27032E

PCSK9 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-2740B6

NGAL/Lipocalin-2 (Human) ELISA Kit (1920T)

20 x 96T

128600

nordicbiosite

ABE-2748AD

ACVR IIA (Soluble) (Human) ELISA KIT (460T)

5 x 96T

57000

nordicbiosite

ABE-276-02

Endothelial Lipase (Mouse/Rat) ELISA Kit

96T

14200

nordicbiosite

ABE-27D12D

Chromogranin A (19-146) (Rat/Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-281AF0

Erythroferrone/Myonectin/CTRP15/FAM132B (Human) ELISA Kit

96T

18200

nordicbiosite

ABE-295155

Gelsolin, Western Blot Kit (5-10 runs)

5-10 runs

17200

nordicbiosite

ABE-296937

LAMp-1/CD107a (Soluble)(Human) ELISA Kit

96T

14200

nordicbiosite

ABE-29882F

Cerebellin-1 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-299B77

WFDC2/HE4/WAP5 (Human) ELISA Kit

5 x 96T

58000

nordicbiosite

ABE-29C372

EphB6 (Soluble)(Human) ELISA Kit

96T

14400

nordicbiosite

ABE-2A1B9A

Human Apolipoprotein H (APOH) Control

1 vial

3800

nordicbiosite

ABE-2A26E0

CTRP-15/Myonectin (Rat, Mouse) ELISA Kit

96T

16000

nordicbiosite

ABE-2A5525

Soluble E-Cadherin/CD324 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-2A8507

Her2/ErbB2 (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-2A9892

Interferon alpha (IFN alpha)(Human) ELISA Kit

96T

12000

nordicbiosite

ABE-2AAA41

sTNFRI (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-2AE315

Beta NGF (Human) Ultrasensitice ELISA Kit

96T

13000

nordicbiosite

ABE-2AF08E

Secreted Klotho (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-2B225F

FGF-9 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-2BE5E5

Autotaxin/ENPP2 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-2C4CB1

TREM-1 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-2C70AB

Albumin (Serum) (Mouse) ELISA Kit

96T

13000

nordicbiosite

ABE-2CD858

FGF-19 (Human) Ultrasensitive ELISA Kit

96T

15200

nordicbiosite

ABE-2D7447

LIGHT/TNFSF14 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-2E372D

Serum Amyloid A-4 Protein (SAA4)(Human) ELISA Kit

96T

14200

nordicbiosite

ABE-2E84AB

VSNL1 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-2E89D6

Endothelial Lipase (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-2F431A

C1 Inhibiotr (C1-INH)/Serpin G1 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-2FB530

High Sensitive C Reactive Protein (hs-CRP) (Human) ELISA Kit

5 x 96T

35400

nordicbiosite

ABE-30-03

Fatty Acid Binding Protein 4 (FABP4)/Adipocyte-type FABP (Rat/Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-30-09

Fatty Acid Binding Protein 4 (FABP4 ) /Adipocyte-Type (AFABP) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-309-01

Angiopoietin-like 4 (ANGPTL-4) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-311-01

Angiopoietin-like 3 (ANGPTL-3) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-311785

IL-2 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-313-03

Acrogranin (GRN) (Mouse/Rat) ELISA KIT

96T

14200

nordicbiosite

ABE-313-06

Acrogranin (GRN) (Human) ELISA KIT

96T

14200

nordicbiosite

ABE-313A44

TGF-beta R II (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-31B19A

IL-1RN (Secreted) (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-31BD09

Pepsinogen C/Pepsinogen II (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-31ED6B

TGF-beta 1 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-32DE

PBEF1/Visfatin (Human) ELISA Kit (15 x 96T) (15 plates)

SET

26400

nordicbiosite

ABE-3276C9

Delta Like Protein 1 (DLL1) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-32800E

CD112 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-329CA9

Granulysin (GNLY)(Human) ELISA Kit

96T

14200

nordicbiosite

ABE-32CC07

REG4 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-330FD7

Osteoponin (OPN) (Human) ELISA Kit (192T)

2 x 96T

22200

nordicbiosite

ABE-339675

Myeloperoxidase (MPO) Antibody Set for ELISA

SET

18200

nordicbiosite

ABE-33BDEA

CD163 (Soluble) (Human) ELISA Kit

96T

24000

nordicbiosite

ABE-3478BE

CTRP-3 (Human) ELISA Kit, Doubles Abs

96T

13800

nordicbiosite

ABE-350A38

uPAR/CD87, Ultr-sensitive Soluble (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-352D96

MMP9/NGAL (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-355-01

Leukemia Inhibitory Factor (LIF) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-35FACB

Angiotensinogen (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-363961

MST1 (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-36D4

Progranulin (PGRN)/Granulin (GRN) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-36B543

Soluble TIM-3/HAVCR2 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-37F16C

High Sensitive C Reactive Protein (CRP) (Human) ELISA Development Set

SET

19200

nordicbiosite

ABE-383E4E

Rat MGFE8 ELISA Kit

96T

14200

nordicbiosite

ABE-38834D

IL-17A (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-388B04

Fatty Acid Binding Protein 4 (FABP4 ) /Adipocyte-Type (AFABP) (Human) ELISA Kit (960T)

10 x 96T

82800

nordicbiosite

ABE-392109

Fetuin A (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-396-06

CTRP7 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-39C5E3

ACVR IIB (Soluble) (Human) ELISA KIT

96T

15200

nordicbiosite

ABE-3AA15B

Fatty Acid Binding Protein 4 (FABP4 ) /Adipocyte-Type (AFABP) (Human) ELISA Kit (480T)

5 x 96T

51000

nordicbiosite

ABE-3AEEBF

CD48 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-3AF225

CCL8/MPC-2 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-3AF884

Lipocalin-2/NGAL (Human) ELISA Developemt Set

Set

19200

nordicbiosite

ABE-3B1CC8

Soluble Flt-1/VEGF-R1 (Human) ELISA Kit (serum and plasma samples)

96T

13000

nordicbiosite

ABE-3B2F6D

CTRP-10 (Rat/Mouse/Human) ELISA Kit

96T

14200

nordicbiosite

ABE-3B383B

CTRP-15/Myonectin (Human) ELISA Kit

96T

18200

nordicbiosite

ABE-3BA0AA

Soluble TREM-1 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-3C8687

Arginase 1 (ARG1) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-3C9179

IL-13 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-3CACBC

Gastric Lipase (Human) ELISA Kit

96T

20000

nordicbiosite

ABE-3D0188

MMP8 (Human) (Total) ELISA Kit

96T

13000

nordicbiosite

ABE-3D4242

CD163 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-3D7E59

CD40 Ligand (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-3E2927

CD209 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-3E7ABB

CD306/LAIR2 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-3E9A13

Renin (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-3EE62A

ADAM15 (Soluble) (Human) ELISA KIT

96T

13800

nordicbiosite

ABE-3F18F2

FSTL1 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-3F3245

Basic FGF/FGF-2 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-402024

IL-13 sR alpha 1 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-40213C

Soluble VEGF R3 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-40483B

ECM-1 (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-40C809

ACVR IIB (Soluble) (Human) ELISA KIT (460T)

460T

57000

nordicbiosite

ABE-413961

RANK (Soluble) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-414843

FGF-23 (Human) ELISA Kit (480T)

5 x 96T

54000

nordicbiosite

ABE-415597

Soluble Flt-1/VEGF-R1 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-41E222

s-E Selectin (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-425891

Blocking Solution (Dry milk base)(Ready to use)

100mL

2200

nordicbiosite

ABE-427-01

CXCL-1/GRO alpha (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-428A02

Soluble IL-1R II (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-42D6FB

TGF-beta 3 (Human( ELISA Kit

96T

13000

nordicbiosite

ABE-43BD6B

BDNF Pro (Human, Mouse, Rat)  ELISA Kit

96T

13000

nordicbiosite

ABE-4429FA

Alpha Fetoprotein (AFP) (Human) ELISA Set

15 x 96 T

19200

nordicbiosite

ABE-44C3EA

Growth Hormone (gGH) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-451A9C

CD5L/AIM (Apoptsis Inhibitor of Macrophages)(Soluble) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-460756

Endocan/Endothelial cell-specific molecule-1 (ESM1) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-4745B9

TREM-2 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-47602E

Corin (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-47F28A

Annexin V-FITC Apoptosis Assay Kit

25 Tests

3400

nordicbiosite

ABE-488864

Thrombospondin-2 (TSP1) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-48A1A4

Arginase 1 (ARG1) (Human) ELISA Kit (960T)

960T

114800

nordicbiosite

ABE-48A53D

High Sensitive Stanniocalcin-1 (STC-1) (Human) ELISA Kit

96T

17200

nordicbiosite

ABE-48FBDF

CD117/ c-Kit (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-49C8D0

Osteomodulin (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-49D557

Dilution Buffer (Ready to use)

60mL

1400

nordicbiosite

ABE-49DF48

TNF alpha (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-4A66B3

Anterior Gradient 2 (AGR2) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-4A7229

Kallikrein 13 (KLK13) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-4B29A5

HGF (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-4B46E9

CTRP-13 (Human, Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-4BA801

Fetuin A (Mouse) ELISA Kit (192T)

2 x 96T

22200

nordicbiosite

ABE-4BD4F6

Dilution Buffer (Ready to use)

60mL

1600

nordicbiosite

ABE-4C119A

MMP8 (Human) (Total) ELISA Kit

96T

13000

nordicbiosite

ABE-4C2125

TIMP-1 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-4C2B34

CXCL-13 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-4CA0DE

Goat Anti Rabbit lgG HRP Conjugate (4000-6000x)

50mL

2600

nordicbiosite

ABE-4CE823

Albumin (Serum) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-4D37DF

Her3/ErbB3 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-4D3CDD

Soluble Klotho (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-4D9F7E

Alpha-2 Antiplasmin (A2AP)/Serpin F2 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-4DDE27

Soluble BCAM/CD239 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-4E10C1

MMP9 (Human) (Total) ELISA Kit

96T

13000

nordicbiosite

ABE-4E3BFE

Cartilage Oligomeric Matrix Protein (COMP) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-4E47BF

LDL-PLA2/PLA2G7 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-4E9E80

Glial Fibrillary Acidic Protein (GFAP) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-4F81EF

Activin A (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-4F9FD7

Dilution Buffer (Ready to use)

60mL

1400

nordicbiosite

ABE-50-02

Leptin (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-502724

Betatrophin (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-509FC3

CTGF (Human) ELISA Kit

2 x 96T

24000

nordicbiosite

ABE-51785F

IL-6 Receptor (Soluble)(Human) ELISA Kit

96T

12000

nordicbiosite

ABE-519F07

Omentin 1 (Human) ELISA Development Kit

SET

18400

nordicbiosite

ABE-524ADA

sTMFRII (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-528-06

Betatrophin (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-528-08

Betatrophin (Total) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-5378

RBP4 (Ultrasensitive) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-53E45C

E-Selectin/ELAM-1 (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-53FE2C

Betatrophin (Active)(Human) ELISA Kit

96T

13000

nordicbiosite

ABE-5407C4

Endocan/Endothelial cell-specific molecule-1 (ESM1) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-5440E4

Coating Solution

100mL

2000

nordicbiosite

ABE-5441F1

Surfactant Protein D (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-5546D5

EGF (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-560-02

Vaspin (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-560-03

Vaspin (Rat/Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-563AA2

Myeloperoxidase (MPO) (Human) ELISA Kit (480T)

5 x 96T

45000

nordicbiosite

ABE-56A337

STC-1 (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-5704FD

IL-33 (Human) ELISA Kit

96T

10000

nordicbiosite

ABE-571C77

Fetuin A (Human) ELISA Kit (480T)

5 x 96T

49000

nordicbiosite

ABE-574AF0

IL-18 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-582CE2

Resistin (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-584278

Angiopoietin-4 (ANGPT4) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-587260

Endoglin/CD105 (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-591A46

Soluble IL-1R 4/ST2 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-5931B1

Mouse TNFRSF19/TROY ELISA Kit

96T

14200

nordicbiosite

ABE-5968E3

BMP-4 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-59AA59

Human Apolipoprotein H (APOH) Standard

1 vial

3800

nordicbiosite

ABE-59B09F

Cystatin C (Human) ELISA Kit (960T)

10 x 96T

79600

nordicbiosite

ABE-2075

DKK4 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-864E

CCL1 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-9B5E

CD83 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-9B70

Osteoprotegerin (OPG) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-CF86

WFDC2/HE4/WAP5 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-5B6A57

MMP3 (Human) (Total) ELISA Kit

96T

13000

nordicbiosite

ABE-5B6C42

Stathmin1/Metablastin (Human) ELISA Kit (192T)

2 x 96T

32800

nordicbiosite

ABE-5B870B

Anti Albumin (Mouse) IgG Microplate for prepration of mouse IgG free samples

96T

2600

nordicbiosite

ABE-5BD6C0

Arginase 1 (ARG1) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-5C527E

Soluble TLR3 (Mouse) ELISA Kit

96T

20000

nordicbiosite

ABE-5C547D

FABP-3/HFABP (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-5C7FEA

KIM-1/TIM-1 (Rat) ELISA Kit

96T

14200

nordicbiosite

ABE-5CF481

Soluble Neprilysin/CD10 (Human) ELISA Kit

96T

14800

nordicbiosite

ABE-5CFF76

Neuropilin-1 (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-5D2C45

Gastric Intrinsic Factor (GIF) (Human) ELISA Kit

96T

20000

nordicbiosite

ABE-5D3F41

Anti Albumin (Human) IgG Microplate for prepration of human IgG free samples

96T

2600

nordicbiosite

ABE-5E4F70

HGF (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-5E2E

IGF-1 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-5ED245

ANG (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-5F8785

LDLR (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-5FCA93

Lipocalin-1 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-609F1F

Delta Like Protein 4 (DLL4) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-610717

VGF (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-612-01

CD152-CTLA-4 (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-6134FA

Prostasin (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-619428

Megakaryocyte-potentiating Factor (MPF) (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-61B6F0

Streptavidin-HRP Conjugate (100x)

120µL

2000

nordicbiosite

ABE-62201C

BMP-2 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-6225E8

Sortilin (Soluble) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-624306

Vaspin (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-625452

High Sensitive C Reactive Protein (CRP) (Human) ELISA Kit

5 x 96T

33200

nordicbiosite

ABE-62950B

Soluble Vasorin (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-632C46

NOV/CCN3 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-63ADED

Neureguiln-4 (NRG-4) (Soluble) (Human) ELISA Kit

96T

18200

nordicbiosite

ABE-63BBFB

ACVR 2A Fc (Soluble) (Human) ELISA Kit 480T

5 x 96T

78800

nordicbiosite

ABE-63D490

vPA (Human) EILSA Kit

96T

13000

nordicbiosite

ABE-641-01

Decorin (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-649DCA

Beta2-microglobulin (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-64B2A7

IL-17A (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-66-01

Angiopoietin-2 (ANGPT2) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-66BF17

Endothelial Lipase (Human) ELISA Kit (for serum)

96T

14200

nordicbiosite

ABE-67-02

Adiponutrin (Human) ELISA Kit

96T

18200

nordicbiosite

ABE-67789D

CD44 (Soluble) (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-684F97

C Reactive Protein (CRP) (Human) ELISA Kit

96T

7400

nordicbiosite

ABE-686-06

Soluble ICAM-2/CD102(Human) ELISA Kit

96T

13000

nordicbiosite

ABE-687-06

Soluble ICAM-3/CD50 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-68E450

FGF-19 (Human) ELISA Kit

960T

104800

nordicbiosite

ABE-68FE22

NGF beta (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-694AE4

Soluble ST2/IL1RL1 (Human) ELISA Kit (for seum or plasma)

96T

14200

nordicbiosite

ABE-69539F

FABP-3/HFABP (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-697-01

Endoglin/CD105 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-699-01

Cystatin C (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-69B186

FGF-23 (Human) ELISA Kit (192T)

2 x 96T

25800

nordicbiosite

ABE-6A08B2

PBEF1/Visfatin (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-6A4442

Fetuin A (Mouse) ELISA Kit

96T

11200

nordicbiosite

ABE-6A8AE6

Cystatin C (Human) ELISA Kit

96T

11200

nordicbiosite

ABE-6AC9EF

Cadherin-16 (CDD-16) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-6AF115

Pepsinogen A/Pepsinogen I (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-6B1EC5

Galectin-7 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-6B6646

Meteorin Like (METRNL)/Subfatin (Human,Mouse,Rat) ELISA Kit

96T

15200

nordicbiosite

ABE-6B768E

CD14 (Soluble) (Human) Ultrasensitive ELISA Kit

96T

14200

nordicbiosite

ABE-6BA168

TNF alpha (Rat) ELISA Kit

96T

14200

nordicbiosite

ABE-6BE58C

Interferon gamma (IFN gamma) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-6C7125

Anti Albumin (Rat) IgG Microplate for prepration of rat IgG free samples

96T

2600

nordicbiosite

ABE-6D033B

Insulin-Like Growth Factor-1 (ILGF-1) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-6DB8EE

Cadherin-13/H-Cadherin/T-Cadherin (Human) ELISA Kit

192T

34400

nordicbiosite

ABE-6DC1D1

CTRP12 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-6DDF87

IGFBP7 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-6DF2CE

YKL-40 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-6E8C59

Trappin-2/Flafin (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-6EBEBD

Soluble IL-1R I (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-6F2253

Assay Wash Solution (10x)

500mL

4600

nordicbiosite

ABE-6F7090

Pro-BDNF (Human) ELISA Kit

96T

10000

nordicbiosite

ABE-6F7CAE

MIP-1 alpha/CCL3 (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-6FC84B

CTRP-9 (Human) ELISA Kit

96T

13800

nordicbiosite

ABE-701-06

Beta Defensin-1 (BD1) (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-701-08

Beta Defensin-1 (BD1) (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-701-09

Beta Defensin-1 (BD1) (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-701-11

Beta Defensin-1 (BD1) (Human) ELISA Set (5 plates)

SET

15200

nordicbiosite

ABE-70A292

CD14 (Soluble) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-70C213

EGF (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-70D28C

CXCL16 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-714DFB

DPPIV/CD26 (Soluble) (Human) ELISA Kit

480T

71000

nordicbiosite

ABE-719-01

Osteoactivin/GPNMB (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-71D254

Azurocidin/CAP37 (Human) ELISA Kit (960T)

960T

97600

nordicbiosite

ABE-72-03

Periostin/OSF-2 (Mouse) ELISA Kit

96T

16000

nordicbiosite

ABE-72-08

Periostin/OSF-2 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-724-05

Soluble Neprilysin ChemiFluorescence ELISA Kit

96T

14200

nordicbiosite

ABE-724-06

Soluble Neprilysin/CD10 (Human) Ultrasensitive ELISA Kit

96T

14800

nordicbiosite

ABE-728FF1

BMP-7 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-7316C8

MMP1 (Human) (Total) ELISA Kit

96T

13000

nordicbiosite

ABE-738A8A

Soluble Neprilysin/CD10 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-742986

Anterior Gradient 2 (AGR2) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-74C085

IGFBP3 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-74CCC5

IL-6 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-752-01

BDNF (Human, Mouse, Rat)  ELISA Kit

96T

13000

nordicbiosite

ABE-759-01

Osteoponin (OPN) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-7A7A

CTGF (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-75BAFD

Interferon gamma (IFN gamma) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-761-01

Solube CD25/IL-2R alpha (Human) ELISA Kit

96T

13000

nordicbiosite

ABE-766-02

Osteonectom/SPARC (Rat/Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-771666

CTRP-4 (Human) ELISA Kit

96T

15200

nordicbiosite

ABE-777272

IGFBP3 (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-77F1D9

BDNF Pro (Human, Mouse, Rat)  ELISA Kit

96T

13000

nordicbiosite

ABE-78587C

CTRP-6 (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-786E8F

P53 (Total) (Human) ELISA Kit

96T

12000

nordicbiosite

ABE-78C720

Meteorin Like (METRNL)/Subfatin (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-790BFF

Periostin/OSF-2 (Human) ELISA Kit

96T

16000

nordicbiosite

ABE-791807

Basic FGF/FGF-2 (Rat) ELISA Kit

96T

14200

nordicbiosite

ABE-796D5F

Cystatin C (Human) ELISA Set (1440T)

15 plates

19200

nordicbiosite

ABE-7996E3

Regerating Islet-derived 1 alpha (REG1A) (Human) ELISA Kit

96T

14200

nordicbiosite

ABE-7B8266

Soluble ST2/IL1RL1 (Human) ELISA Kit

2 X 96T

22200

nordicbiosite

ABE-7D66AF

Osteoponin (OPN) (Mouse) ELISA Kit

96T

14200

nordicbiosite

ABE-7E78E2

IL-37/IL-1F7 (Human) ELISA Kit

96T

15200

nordicbiosite

 

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。

 

qa-bio E-EF01说明书


内切糖苷酶

– 内切
糖苷酶从清洁制剂中的糖蛋白 – 高度稳定的酶切割完整的聚糖 – 不含甘油,NaCl或其他添加剂,如EDTA。
– 测试所有酶不存在蛋白水解或意外的糖苷活性

内切糖苷酶是用于分析糖蛋白的广泛使用的酶。这些酶从糖蛋白释放完整的聚糖,而外切糖苷酶释放单个单糖(即唾液酸,半乳糖,β-N-乙酰基葡糖胺,甘露糖等)。

PNGase F通常包含在酶组中,因为其切割完整的N-连接聚糖的活性相似,尽管它实际上是酰胺酶。它在天冬酰胺氨基酸和N-连接聚糖的壳二糖核心的个β-N-乙酰葡糖胺糖(GlcNAc)之间切割。Endo F酶和Endo H在和第二GlcNAc之间切割N-连接的聚糖,留下带电荷的残基,其有助于增加去糖基化蛋白质的溶解度,降低蛋白质聚集体沉淀的可能性。PNGase F切割所有N-连接的聚糖,而Endo H和Endo F酶切除特定类型的N-连接聚糖。

酶的这种分类还包括O-糖苷酶,其从丝氨酸或苏氨酸氨基酸中除去核心1O-连接的聚糖(Galβ1,3GalNAcα)。O-连接的聚糖通常含有在分支和线性连接中与半乳糖连接的另外的单糖,需要使用外切糖苷酶如唾液酸酶和半乳糖苷酶来除去额外的糖。

 

Endo F1 从肽和蛋白质中切割出高甘露糖和一些杂合型N-聚糖

 

qa-bio E-EF01说明书

 

 

产品编号 – 酶
E-EF01的量 – 60μLsE 
-EF01-20 – 20μls¹E 
-EF01-200 – 200μls²¹ 
  包括缓冲液
  ² 仅含酶

 

Endo F1,内切糖苷酶F1,内切-β-N-乙酰氨基葡萄糖苷酶F.

Endo F1切割天冬酰胺连接的高甘露糖和一些混合低聚糖。核心岩藻糖基化将活性降低50倍。内切糖苷酶F1将水解含有高甘露糖链的硫酸盐。它在寡糖的二乙酰基壳二糖核心中的两个N-乙酰葡糖胺残基之间切割,产生截短的糖分子,其中一个N-乙酰葡糖胺残基保留在天冬酰胺上。相反,PNGase F完整地去除寡糖。

附加远藤˚F产品
内切糖苷酶F2释放双触角和高甘露糖聚糖(以40X减小的速率)
内切糖苷酶F3将释放triantennarry和岩藻糖基双触角N-聚糖
的远藤˚F多套件包括每个远藤˚F酶和它们的缓冲器中的20个μLs。

重组Elizabethkingia miricola(是脑膜脓毒性金黄)在大肠杆菌

EC 3.2.1.96

Endo F1特异性切割所有天冬酰胺连接的高甘露糖和一些混合低聚糖

内容
在20mM酶的60微升等分试样(1 U)的Tris-HCl,pH 7.5中

包含20μL和60μL包装尺寸:
5x反应缓冲液 – 250 mM磷酸钠,pH 5.5

比活性 > 16U / mg 
活性 > 17U / ml

分子量 32,000道尔顿

建议用法
1.在Eppendorf管中加入高达200μg的糖蛋白。用去离子水调节至38μl终体积。
2.加入10μl5x反应缓冲液5.5 
3.加入2.0μlEndoF1。在37℃孵育1小时。

比活性
定义为在37℃,pH5.5下,在1分钟内催化从1微摩尔变性核糖核酸酶B(RNase B)释放N-连接寡糖所需的酶量。通过SDS-PAGE监测切割(切割的RNase B迁移更快)。

储存储存在4˚C。

稳定性妥善储存至少12个月。几天暴露于环境温度不会降低活动。

纯度内切糖苷酶F1如下测试污染蛋白酶; 将10μg变性的BSA在37℃下与2μL酶一起温育24小时。经处理的BSA的SDS-PAGE分析显示没有降解的迹象。

生产宿主菌株已经过广泛测试,并且不产生任何可检测的糖苷酶。

 

 

 

 

 

 

 

E2F-Luc荧光素酶报告基因质粒(E2F Luciferase Reporter Plasmid)

E2F-Luc荧光素酶报告基因质粒(E2F Luciferase Reporter Plasmid)

产品说明书

FAQ

COA

已发表文献

 

E2F-Luc萤光素酶报告基因(报告基因质粒)(E2F luciferase reporter plasmid)是金畔生物自主研发的用于检测E2F转录活性水平为目的的报告基因。E2F转录因子在细胞周期调控中其重要的作用。研究证明,E2F-1对细胞的生长具有双向调节作用,可以调节细胞的增殖和凋亡。因此,E2F与肿瘤发生、细胞凋亡、肿瘤抑制等均有密切关系。

E2F 报告基因主要用于检测细胞中Cell Cycle信号通路、药物研究、基因过表达和RNAi的表型分析等。

pE2F-Luc是金畔生物改造后的哺乳动物真核表达载体,在其多克隆位点插入了多个E2F结合位点,可以高灵敏度地检测E2F的激活水平。同时,对载体中预测出的其它转录因子以外的结合位点进行了适当的突变,在保持原有功能不变的情况下,增加了质粒的转录因子结合特异性。由于质粒体积减小,使得E2F报告基因更易于转染。

 

质粒图谱

E2F-Luc荧光素酶报告基因质粒(E2F Luciferase Reporter Plasmid) 

 

质粒元件信息

E2F response element (E2F)

32-67

Minimal TA promoter (pTA)

96-108

Luciferase reporter gene

150-1812

SV40 late poly(A) signal

1847-2068

SV40 early promoter

2116-2534

Synthetic neomycin phosphotransferase (Neor) coding region

2559-3353

Synthetic poly(A) signal

3378-3426

Synthetic Beta-lactamase(Ampr) coding region

4541-5401

Synthetic poly(A) signal/transcriptional pause site

5506-5659

 

运输和保存方法

冰袋低温运输。20℃保存保质期1年。

 

注意事项

1)本质粒未经金畔生物允许不得用于任何商业用途,也不得移交给订货人实验室以外的任何人或单位;

2为了您的安全和健康,请穿实验服并戴一次性手套操作

3)本产品仅作科研用途!

4)不提供该质粒序列信息。

 

使用说明

1)首次使用1 µg包装的本产品时,请先取少量本质粒转化大肠杆菌,进行质粒小量、中量或大量抽提后再用于后续用途。抽提获得的质粒可以通过酶切电泳进行鉴定,或通过测序进行鉴定;

2pE2F-Luc可以采用常规转染方法转染哺乳动物细胞。用萤光素酶检测试剂盒或双萤光素酶检测试剂盒进行检测。

 

参考文献

[1] Tang J, Liu J, Li J, et al. Upregulation of SKA3 enhances cell proliferation and correlates with poor prognosis in hepatocellular carcinoma[J]. Oncology Reports, 2021, 45(4): 1-11.

[2] Wu P, Ding B, Ye L, et al. Zhibaidihuang decoction ameliorates cell oxidative stress by regulating the Keap1-Nrf2-ARE signalling pathway[J]. Evidence-Based Complementary and Alternative Medicine, 2020.

[3] Polovic M, Dittmar S, Hennemeier I, et al. Identification of a novel lncRNA induced by the nephrotoxin ochratoxin A and expressed in human renal tumor tissue[J]. Cellular and Molecular Life Sciences, 2018, 75(12): 2241-2256.

[4] Tang J, Liu J, Li J, et al. Upregulation of SKA3 enhances cell proliferation and correlates with poor prognosis in hepatocellular carcinoma[J]. Oncology Reports, 2021, 45(4): 1-11.

[5] Zheng Y, Stamminger T, Hearing P. E2F/Rb family proteins mediate interferon induced repression of adenovirus immediate early transcription to promote persistent viral infection[J]. PLoS pathogens, 2016, 12(1): e1005415.

 

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pGM-CMV Luciferase Reporter Plasmid Positive Control pGM-CMV-Luc荧光素酶报告基因质粒阳性对照

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pGMLR-TK Luciferase Reporter Plasmid(pGMLR-TK海肾荧光素酶报告基因质粒)

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HB211026

 

 

E2F-Luc荧光素酶报告基因质粒(E2F Luciferase Reporter Plasmid)

暂无内容

[1] Wang H, Yu S, Peng H, et al. Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma. J Exp Clin Cancer Res. 2020;39(1):216. Published 2020 Oct 14. doi:10.1186/s13046-020-01725-5(IF:7.068)

 

E2F-Luc萤光素酶报告基因(报告基因质粒)(E2F luciferase reporter plasmid)是金畔生物自主研发的用于检测E2F转录活性水平为目的的报告基因。E2F转录因子在细胞周期调控中其重要的作用。研究证明,E2F-1对细胞的生长具有双向调节作用,可以调节细胞的增殖和凋亡。因此,E2F与肿瘤发生、细胞凋亡、肿瘤抑制等均有密切关系。

E2F 报告基因主要用于检测细胞中Cell Cycle信号通路、药物研究、基因过表达和RNAi的表型分析等。

pE2F-Luc是金畔生物改造后的哺乳动物真核表达载体,在其多克隆位点插入了多个E2F结合位点,可以高灵敏度地检测E2F的激活水平。同时,对载体中预测出的其它转录因子以外的结合位点进行了适当的突变,在保持原有功能不变的情况下,增加了质粒的转录因子结合特异性。由于质粒体积减小,使得E2F报告基因更易于转染。

 

质粒图谱

E2F-Luc荧光素酶报告基因质粒(E2F Luciferase Reporter Plasmid) 

 

质粒元件信息

E2F response element (E2F)

32-67

Minimal TA promoter (pTA)

96-108

Luciferase reporter gene

150-1812

SV40 late poly(A) signal

1847-2068

SV40 early promoter

2116-2534

Synthetic neomycin phosphotransferase (Neor) coding region

2559-3353

Synthetic poly(A) signal

3378-3426

Synthetic Beta-lactamase(Ampr) coding region

4541-5401

Synthetic poly(A) signal/transcriptional pause site

5506-5659

 

运输和保存方法

冰袋低温运输。20℃保存保质期1年。

 

注意事项

1)本质粒未经金畔生物允许不得用于任何商业用途,也不得移交给订货人实验室以外的任何人或单位;

2为了您的安全和健康,请穿实验服并戴一次性手套操作

3)本产品仅作科研用途!

4)不提供该质粒序列信息。

 

使用说明

1)首次使用1 µg包装的本产品时,请先取少量本质粒转化大肠杆菌,进行质粒小量、中量或大量抽提后再用于后续用途。抽提获得的质粒可以通过酶切电泳进行鉴定,或通过测序进行鉴定;

2pE2F-Luc可以采用常规转染方法转染哺乳动物细胞。用萤光素酶检测试剂盒或双萤光素酶检测试剂盒进行检测。

 

参考文献

[1] Tang J, Liu J, Li J, et al. Upregulation of SKA3 enhances cell proliferation and correlates with poor prognosis in hepatocellular carcinoma[J]. Oncology Reports, 2021, 45(4): 1-11.

[2] Wu P, Ding B, Ye L, et al. Zhibaidihuang decoction ameliorates cell oxidative stress by regulating the Keap1-Nrf2-ARE signalling pathway[J]. Evidence-Based Complementary and Alternative Medicine, 2020.

[3] Polovic M, Dittmar S, Hennemeier I, et al. Identification of a novel lncRNA induced by the nephrotoxin ochratoxin A and expressed in human renal tumor tissue[J]. Cellular and Molecular Life Sciences, 2018, 75(12): 2241-2256.

[4] Tang J, Liu J, Li J, et al. Upregulation of SKA3 enhances cell proliferation and correlates with poor prognosis in hepatocellular carcinoma[J]. Oncology Reports, 2021, 45(4): 1-11.

[5] Zheng Y, Stamminger T, Hearing P. E2F/Rb family proteins mediate interferon induced repression of adenovirus immediate early transcription to promote persistent viral infection[J]. PLoS pathogens, 2016, 12(1): e1005415.

 

相关产品

产品名称

货号

规格

Dual Luciferase Reporter Gene Assay Kit 双荧光素酶报告基因检测试剂盒HOT

11402JP60/80

100/1000 T

Luciferase Reporter Gene Assay Kit 萤火虫荧光素酶报告基因检测试剂盒

11401JP60/76/80

100/500/1000 T

pGM-CMV Luciferase Reporter Plasmid Positive Control pGM-CMV-Luc荧光素酶报告基因质粒阳性对照

11556JP03

1 μg

Luciferase Reporter Plasmid negative control (荧光素酶报告基因质粒阴性对照)

11555JP03

1 μg

pGMLR-TK Luciferase Reporter Plasmid(pGMLR-TK海肾荧光素酶报告基因质粒)

11557JP03

1 μg

pGMLR-CMV Luciferase Reporter Plasmid(pGMLR-CMV海肾荧光素酶报告基因质粒)

11558JP03

1 μg

 

HB211026

 

 

E2F-Luc荧光素酶报告基因质粒(E2F Luciferase Reporter Plasmid)

暂无内容

[1] Wang H, Yu S, Peng H, et al. Long noncoding RNA Linc00337 functions as an E2F1 co-activator and promotes cell proliferation in pancreatic ductal adenocarcinoma. J Exp Clin Cancer Res. 2020;39(1):216. Published 2020 Oct 14. doi:10.1186/s13046-020-01725-5(IF:7.068)