pLVX-DsRed-Monomer-N1载体


 pLVX-DsRed-Monomer-N1载体

型号 载体名称 出品公司 载体用途
VMC0379 pLVX-DsRed-Monomer-N1 Clontech 慢病毒载体

Description

pLVX-DsRed-Monomer-N1 is an HIV-1-based, lentiviral expression vector that allows you 

to express your gene of interest fused to DsRed-Monomer, a monomeric mutant of the 

Discosoma sp. red fluorescent protein. Genes cloned into the multiple cloning site (MCS), 

located upstream of the DsRed-Monomer coding sequence, are expressed as N-terminal 

fusions of the DsRed-Monomer protein. Expression of the fusion protein is driven by the 

constitutively active human cytomegalovirus immediate early promoter (P

CMV IE) located 

just upstream of the MCS. Lentiviral particles derived from the vector allow the expression 

of DsRed-Monomer fusion proteins in virtually any cell type, including primary cells. The 

unmodified vector expresses DsRed-Monomer, and may be used to produce marker virus to 

optimize infection protocols.

质粒图谱: 

DsRed Tag Mouse mAb (HRP Conjugated)(DsRed Tag 小鼠单抗)(AF2909)

DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated)
DsRed Tag  Mouse mAb (HRP Conjugated)(DsRed Tag  小鼠单抗)(AF2909) 一键复制产品信息

产品编号: AF2909

产品包装:50μl
选择包装

50μl

说明书下载

388.00 10

价格: ¥ 388.00

促销价: ¥ 388.00

促销价: ¥ 388.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
AF2909 DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl 388.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Mouse WB, IHC, ICC DsRed-tagged protein in all species

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:500-1:2000 1:50-1:100 1:50-1:100

抗体详细信息如下:

About this Antibody
Name DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated)
Category Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody
Isotype IgG1
Purificationmethod Affinity purification
Positive samples
Cellular location
Customer validation
About the Immunogen
Immunogen Recombinant DsRed protein expressed in E. coli
Sequence
Gene ID
SwissProt
Synonyms
Category Polypeptide protein tag
Background DsRed is a red fluorescent protein cloned by homology to Green Fluorescent Protein (GFP) from Discosoma coral. DsRed exhibits excellent resistance to pH extremes and photobleaching and causes a strong red-shift from 558 nm to 583 nm once matured. Immature DsRed shows GFP-like excitation and emission maxima. The contrast created through GFP and DsRed spectral shifts allows for a powerful dual reporter system.

包装清单:

产品编号 产品名称 包装
AF2909 DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份

保存条件:

DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

请根据抗体的实际用途选择相应的使用方法。
1.Western检测:
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗,4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作,无须再孵育二抗。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。

相关产品:

产品编号 产品名称 包装
AF2852 Flag Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2855 Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2858 HA Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2861 HA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2864 Myc Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2867 Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2870 His Tag (C-terminal Specific) Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2873 His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2876 His Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2879 His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2882 GFP Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2885 GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2888 GST Tag Mouse Monoclonal Antibody 50μl/200μl
AF2891 GST Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2894 V5 Tag Mouse Monoclonal Antibody 50μl/200μl
AF2897 V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2900 Glu-Glu Tag Mouse Monoclonal Antibody 50μl
AF2906 DsRed Tag Mouse Monoclonal Antibody 50μl
AF2909 DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2912 MBP Tag Mouse Monoclonal Antibody 50μl
AF2915 MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2918 PA Tag Mouse Monoclonal Antibody 50μl
AF2921 PA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2924 StrepII Tag Mouse Monoclonal Antibody 50μl
AF2927 StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2930 S Tag Mouse Monoclonal Antibody 50μl
AF2933 S Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2936 TEV-His Tag Mouse Monoclonal Antibody 50μl
AF2939 TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2942 Target Tag Mouse Monoclonal Antibody 50μl
AF2945 Target Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2948 TRX Tag Mouse Monoclonal Antibody 50μl
AF2951 TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2954 AVI Tag Mouse Monoclonal Antibody 50μl
AF2957 AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl

DsRed Tag  Mouse mAb (HRP Conjugated)(DsRed Tag  小鼠单抗)(AF2909)
DsRed Tag  Mouse mAb (HRP Conjugated)(DsRed Tag  小鼠单抗)(AF2909)

物质安全数据单
输入右侧验证码点击下载:

DsRed Tag  Mouse mAb (HRP Conjugated)(DsRed Tag  小鼠单抗)(AF2909)

DsRed Tag  Mouse mAb (HRP Conjugated)(DsRed Tag  小鼠单抗)(AF2909)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
标签抗体
HRP标记一抗

使用本产品的文献:

DsRed Tag Mouse mAb(DsRed Tag 小鼠单抗)(AF2906)

DsRed Tag Mouse Monoclonal Antibody
DsRed Tag  Mouse mAb(DsRed Tag  小鼠单抗)(AF2906) 一键复制产品信息

产品编号: AF2906

产品包装:50μl
选择包装

50μl

说明书下载

299.00 10

价格: ¥ 299.00

促销价: ¥ 299.00

促销价: ¥ 299.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
AF2906 DsRed Tag Mouse Monoclonal Antibody 50μl 299.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Mouse WB, IP, IF, IHC, ICC DsRed-tagged protein in all species

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:500-1:2000 1:50 1:50-1:100 1:50-1:100 1:50-1:100

抗体详细信息如下:

About this Antibody
Name DsRed Tag Mouse Monoclonal Antibody
Category Mouse Monoclonal Antibody (mAb); Primary antibody
Isotype IgG1
Purificationmethod Affinity purification
Positive samples
Cellular location
Customer validation
About the Immunogen
Immunogen Recombinant DsRed protein expressed in E. coli
Sequence
Gene ID
SwissProt
Synonyms
Category Polypeptide protein tag
Background DsRed is a red fluorescent protein cloned by homology to Green Fluorescent Protein (GFP) from Discosoma coral. DsRed exhibits excellent resistance to pH extremes and photobleaching and causes a strong red-shift from 558 nm to 583 nm once matured. Immature DsRed shows GFP-like excitation and emission maxima. The contrast created through GFP and DsRed spectral shifts allows for a powerful dual reporter system.

包装清单:

产品编号 产品名称 包装
AF2906 DsRed Tag Mouse Monoclonal Antibody 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份

保存条件:

DsRed Tag Mouse Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

请根据抗体的实际用途选择相应的使用方法。
1. Western检测:
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品:

产品编号 产品名称 包装
AF2852 Flag Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2855 Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2858 HA Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2861 HA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2864 Myc Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2867 Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2870 His Tag (C-terminal Specific) Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2873 His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2876 His Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2879 His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2882 GFP Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2885 GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2888 GST Tag Mouse Monoclonal Antibody 50μl/200μl
AF2891 GST Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2894 V5 Tag Mouse Monoclonal Antibody 50μl/200μl
AF2897 V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2900 Glu-Glu Tag Mouse Monoclonal Antibody 50μl
AF2906 DsRed Tag Mouse Monoclonal Antibody 50μl
AF2909 DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2912 MBP Tag Mouse Monoclonal Antibody 50μl
AF2915 MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2918 PA Tag Mouse Monoclonal Antibody 50μl
AF2921 PA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2924 StrepII Tag Mouse Monoclonal Antibody 50μl
AF2927 StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2930 S Tag Mouse Monoclonal Antibody 50μl
AF2933 S Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2936 TEV-His Tag Mouse Monoclonal Antibody 50μl
AF2939 TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2942 Target Tag Mouse Monoclonal Antibody 50μl
AF2945 Target Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2948 TRX Tag Mouse Monoclonal Antibody 50μl
AF2951 TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2954 AVI Tag Mouse Monoclonal Antibody 50μl
AF2957 AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl

DsRed Tag  Mouse mAb(DsRed Tag  小鼠单抗)(AF2906)
DsRed Tag  Mouse mAb(DsRed Tag  小鼠单抗)(AF2906)

物质安全数据单
输入右侧验证码点击下载:

DsRed Tag  Mouse mAb(DsRed Tag  小鼠单抗)(AF2906)

DsRed Tag  Mouse mAb(DsRed Tag  小鼠单抗)(AF2906)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
标签抗体

使用本产品的文献:

RNAi-Ready pSIREN-Retro Q-DsRed-Express

RNAi载体,RNAi-Ready pSIREN-Retro Q-DsRed-Express载体 

RNAi-Ready pSIREN-Retro Q-DsRed-Express

型号 载体名称 出品公司 载体用途
VRC0346 RNAi-Ready pSIREN-Retro Q-DsRed-Express Clontech RNAi载体
载体描述: 

RNAi-Ready pSIREN-RetroQ-DsRed-Express is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter (PU6; RNA Pol III-dependent). RNAi-Ready pSIREN-RetroQ-DsRed-Express is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when a ds DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQDsRed-Express construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA.

质粒图谱: