Cyclopamine(环巴胺) 异甾体类生物碱 Hedgehog(Hh)抑制剂|CAS 4449-51-8

Cyclopamine(环巴胺) 异甾体类生物碱 Hedgehog(Hh)抑制剂|CAS 4449-51-8

产品说明书

FAQ

COA

已发表文献

Cyclopamine(环巴胺)是从藜芦属植物内分离得到的一种异甾体类生物碱, 主要存在于百合科植物中北美山藜芦、印第安鹿食草、毛叶藜芦、伊贝四种植物中。Cyclopamine对Hedgehog信号通路有抑制作用,其IC50值为46 nM。Cyclopamine 还是一种选择性的Smo抑制剂。Cyclopamine是一种特异性Hedgehog (Hh) 信号通路拮抗剂,对包括基底细胞瘤、神经胶质瘤、小细胞肺癌、胃癌、乳腺癌等恶性肿瘤均有显著的抑制活性,是一种潜在的抗肿瘤药物。

 

产品性质

英文别名(English Synonym)

11-Deoxojervine; Shh Signaling Antagonist I

中文名称 (Chinese Name)

环巴胺;环杷明;11-去氧芥芬胺

靶点(Target)

人内源性代谢物,Human Endogenous Metabolite

CAS号(CAS NO.)

4449-51-8

分子式(Formula)

C27H41NO2

分子量(Molecular Weight)

411.62

外观(Appearance)

类白色粉末

纯度(Purity)

≥98%

溶解性(Solubility)

溶于DMSO:5 mg/mL (12.15 mM);溶于乙醇:10 mg/mL (24.3 mM)

结构式(Structure)

Cyclopamine(环巴胺) 异甾体类生物碱 Hedgehog(Hh)抑制剂|CAS 4449-51-8

 

运输和保存方法

冰袋运输。粉末直接保存于-25~-15℃,有效期2年。建议干燥避光保存,避免反复冻融。

 

注意事项

1. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

2. 粉末溶解前请先短暂离心,以保证产品全在管底。

3. 请勿吸入、吞咽或者直接接触皮肤和眼睛。

4. 本产品仅用于科研用途,禁止用于人身上。

 

使用浓度

【具体使用浓度请参考相关文献,并根据自身实验条件(如实验目的,细胞种类,培养特性等)进行摸索和优化。

 

使用方法(数据来自于公开发表的文献,仅供参考)

(一)细胞实验(体外实验)

为了检测Cyclopamine对人食道癌细胞的影响,接种6×105个数量的人食道癌细胞系EC9706至96孔培养板中,添加不同浓度(2.5, 5, 10, 20 μM)Cyclopamine工作液,并培养孵育12~72 h。经MTT实验验证,Cyclopamine以剂量和时间依赖性的方式有效抑制细胞增殖[1]

Cyclopamine是一种Hedgehog信号通路拮抗剂,其IC50值为46 nM。Cyclopamine可以抑制细胞生长。Cyclopamine( 3 μM)通过抑制PTCHmRNA的表达,下调Hh信号通路活性和抑制消化道肿瘤细胞生长。Cyclopamine是一种甾体生物碱,通过与Smo直接相互作用抑制Hh信号通路,抑制人类Smo受体活性,其IC50值为280 nM。[3]

(二)动物实验(体内实验)

为了检测Cyclopamine抗肿瘤的潜在作用,100 μL 2×106Panc 05.04胰腺癌细胞皮下注射到4~6周龄CD-1裸鼠,待成瘤后,给予皮下注射连续7天,每天一次1.2 mg /小鼠的Cyclopamine,与未给予Cyclopamine对照组相比,每周测量的肿瘤体积显著变小[2] 将人的胰腺癌细胞移植到裸鼠体内后,再用Cyclopamine(1.2 mg)处理肿瘤裸鼠7天,结果显示Cyclopamine显著抑制肿瘤转移,诱导肿瘤细胞凋亡。[3]

 

参考文献

[1]. Yu J, et al. Cyclopamine Suppresses Human Esophageal Carcinoma Cell Growth by Inhibiting Glioma-Associated Oncogene Protein-1, a Marker of Human Esophageal Carcinoma Progression. Med Sci Monit. 2019 Feb 26;25:1518-1525.

[2]. Thayer SP, et al. Hedgehog is an early and late mediator of pancreatic cancer tumorigenesis. Nature. 2003 Oct 23;425(6960):851-6.

[3]. Yang DH, et al. Absorptive constituents and their metabolites in drug-containing urine samples from Wuzhishan miniature pigs orally administered with Buyang Huanwu decoction. J Nat Med. 2014 Jan;68(1):11-21. 

HB220218

Cyclopamine(环巴胺) 异甾体类生物碱 Hedgehog(Hh)抑制剂|CAS 4449-51-8

暂无内容

Cyclopamine(环巴胺) 异甾体类生物碱 Hedgehog(Hh)抑制剂|CAS 4449-51-8

暂无内容

Cyclopamine(环巴胺)是从藜芦属植物内分离得到的一种异甾体类生物碱, 主要存在于百合科植物中北美山藜芦、印第安鹿食草、毛叶藜芦、伊贝四种植物中。Cyclopamine对Hedgehog信号通路有抑制作用,其IC50值为46 nM。Cyclopamine 还是一种选择性的Smo抑制剂。Cyclopamine是一种特异性Hedgehog (Hh) 信号通路拮抗剂,对包括基底细胞瘤、神经胶质瘤、小细胞肺癌、胃癌、乳腺癌等恶性肿瘤均有显著的抑制活性,是一种潜在的抗肿瘤药物。

 

产品性质

英文别名(English Synonym)

11-Deoxojervine; Shh Signaling Antagonist I

中文名称 (Chinese Name)

环巴胺;环杷明;11-去氧芥芬胺

靶点(Target)

人内源性代谢物,Human Endogenous Metabolite

CAS号(CAS NO.)

4449-51-8

分子式(Formula)

C27H41NO2

分子量(Molecular Weight)

411.62

外观(Appearance)

类白色粉末

纯度(Purity)

≥98%

溶解性(Solubility)

溶于DMSO:5 mg/mL (12.15 mM);溶于乙醇:10 mg/mL (24.3 mM)

结构式(Structure)

Cyclopamine(环巴胺) 异甾体类生物碱 Hedgehog(Hh)抑制剂|CAS 4449-51-8

 

运输和保存方法

冰袋运输。粉末直接保存于-25~-15℃,有效期2年。建议干燥避光保存,避免反复冻融。

 

注意事项

1. 为了您的安全和健康,请穿实验服并戴一次性手套操作。

2. 粉末溶解前请先短暂离心,以保证产品全在管底。

3. 请勿吸入、吞咽或者直接接触皮肤和眼睛。

4. 本产品仅用于科研用途,禁止用于人身上。

 

使用浓度

【具体使用浓度请参考相关文献,并根据自身实验条件(如实验目的,细胞种类,培养特性等)进行摸索和优化。

 

使用方法(数据来自于公开发表的文献,仅供参考)

(一)细胞实验(体外实验)

为了检测Cyclopamine对人食道癌细胞的影响,接种6×105个数量的人食道癌细胞系EC9706至96孔培养板中,添加不同浓度(2.5, 5, 10, 20 μM)Cyclopamine工作液,并培养孵育12~72 h。经MTT实验验证,Cyclopamine以剂量和时间依赖性的方式有效抑制细胞增殖[1]

Cyclopamine是一种Hedgehog信号通路拮抗剂,其IC50值为46 nM。Cyclopamine可以抑制细胞生长。Cyclopamine( 3 μM)通过抑制PTCHmRNA的表达,下调Hh信号通路活性和抑制消化道肿瘤细胞生长。Cyclopamine是一种甾体生物碱,通过与Smo直接相互作用抑制Hh信号通路,抑制人类Smo受体活性,其IC50值为280 nM。[3]

(二)动物实验(体内实验)

为了检测Cyclopamine抗肿瘤的潜在作用,100 μL 2×106Panc 05.04胰腺癌细胞皮下注射到4~6周龄CD-1裸鼠,待成瘤后,给予皮下注射连续7天,每天一次1.2 mg /小鼠的Cyclopamine,与未给予Cyclopamine对照组相比,每周测量的肿瘤体积显著变小[2] 将人的胰腺癌细胞移植到裸鼠体内后,再用Cyclopamine(1.2 mg)处理肿瘤裸鼠7天,结果显示Cyclopamine显著抑制肿瘤转移,诱导肿瘤细胞凋亡。[3]

 

参考文献

[1]. Yu J, et al. Cyclopamine Suppresses Human Esophageal Carcinoma Cell Growth by Inhibiting Glioma-Associated Oncogene Protein-1, a Marker of Human Esophageal Carcinoma Progression. Med Sci Monit. 2019 Feb 26;25:1518-1525.

[2]. Thayer SP, et al. Hedgehog is an early and late mediator of pancreatic cancer tumorigenesis. Nature. 2003 Oct 23;425(6960):851-6.

[3]. Yang DH, et al. Absorptive constituents and their metabolites in drug-containing urine samples from Wuzhishan miniature pigs orally administered with Buyang Huanwu decoction. J Nat Med. 2014 Jan;68(1):11-21. 

HB220218

Cyclopamine(环巴胺) 异甾体类生物碱 Hedgehog(Hh)抑制剂|CAS 4449-51-8

暂无内容

Cyclopamine(环巴胺) 异甾体类生物碱 Hedgehog(Hh)抑制剂|CAS 4449-51-8

暂无内容

LC Laboratories公司Cyclopamine


LC Laboratories公司Cyclopamine

简要描述:LC Laboratories公司提供信号转导,调节蛋白亚细胞定位试剂(抗生素)产品蛋白抑制剂产品

详细介绍

产品咨询

公司:www.lclabs.com

LC Laboratories公司提供信号转导,调节蛋白亚细胞定位试剂(抗生素)产品蛋白抑制剂产品。 从下表可以看出LC价格是zui低的

      Purity and Price Comparisons **

C-8700   Cyclopamine, Free Base

Prices in US Dollars ($)

Company

Cat. No.

Purity
Claim

1 mg

2 mg

5 mg

10 mg

20 mg

25 mg

50 mg

100 mg

300 mg

500 mg

1 g

2 g

 

LC Labs

C-8700 

99 %

 

 

 

 

 

122 

214 

375 

895 

1250 

1980 

3630 

Calbiochem

239803 

97 %  

139 

 

 

 

 

 

 

 

 

 

 

 

Sigma

C4116 

98 %  

183 

 

713 

 

 

 

 

 

 

 

 

 

Enzo

BML-GR334 

98 %  

95 

 

381 

 

 

 

 

 

 

 

 

 

Tocris

1623 

97 %  

159 

 

 

 

 

 

 

 

 

 

 

 

AG Scientific

C-1405 

98 %  

 

 

89 

 

 

195 

 

 

 

 

 

 

Axxora

BML-GR334 

98 %  

95 

 

381 

 

 

 

 

 

 

 

 

 

Indofine

08-1995 

99 %  

 

 

 

 

 

375 

710 

 

 

 

 

 

LKT Labs

C9710 

98 %  

135 

 

534 

 

 

 

 

 

 

 

 

 

Santa Cruz Biotech

sc-200929 

98 %  

90 

 

360 

 

 

 

 

 

 

 

 

 

Active Biochem

A-1057 

99 %  

 

 

95 

150 

250 

 

420 

 

 

 

 

 

US Biological

C8845 

99 %  

210 

 

 

 

 

 

 

 

 

 

 

 

BioVision

1578 

99 %  

 

 

195 

 

 

 

 

 

 

 

 

 

Logan Nat. Products

cycl 

99 %  

 

 

 

100 

 

200 

350 

600 

 

2000 

3500 

 

BioShop Canada

CYC870 

99 %  

137 

 

327 

446 

 

 

 

 

 

 

 

 

GenDepot

BC019 

99 %  

 

 

 

 

 

216 

340 

620 

 

 

 

 

R&D Systems

1623 

97 %  

159 

 

 

 

 

 

 

 

 

 

 

 

Ascent Scientific

Asc-392 

97 %  

75 

 

312 

 

 

 

 

 

 

 

 

 

Alfa Aesar

J61528 

99 %  

 

 

 

 

 

278 

492 

900 

 

 

 

 

Selleck Chemicals

S1146 

99 %  

 

 

70 

120 

 

210 

370 

 

 

 

 

 

StressMarq

SIH-226 

99 %  

89 

 

355 

 

 

 

 

 

 

 

 

 

Stemgent

04-0022 

99 %  

 

159 

 

 

 

 

 

 

 

 

 

 

JiHe Pharmaceutica

JH-1057 

99 %  

 

 

95 

150 

250 

 

420 

 

 

 

 

 

antibodies-online

ABIN412154 

99 %  

 

 

195 

 

 

 

 

 

 

 

 

 

biorbyt.com

orb61011 

99 %  

 

 

 

 

 

240 

384 

640 

 

 

 

 

Clear Synth Labs

CS-T-05627 

98 %  

 

 

 

600 

 

 

 

 

 

 

 

 

Reagents Direct

29-O52 

99 %  

 

 

199 

 

 

 

 

 

 

 

 

 

biokits.com

1578 

99 %  

 

 

195 

 

 

 

 

 

 

 

 

 

HuoLi Biochem

A-1057 

99 %  

 

 

95 

150 

250 

 

420 

 

 

 

 

 

Cellagen Technology

C2925 

98 %  

 

129 

 

 

 

 

 

 

 

 

 

 

AddexBio

IN0029 

98 %  

 

 

100 

150 

 

250 

420 

 

 

 

 

 

Synthonix

C3393 

99 %  

 

 

 

 

 

180 

350 

650 

 

2600 

5000 

 

Savings with
LC Labs

 

 

 

 

 

 

 

32 – 67%

37 – 70%

38 – 58%

 

38 – 52%

43 – 60%

 

** Our prices are lower than other vendors’, but there is no compromise in quality.  Read how we are able to accomplish this:  Product Quality Discussion.

NOTES:
1.  The purpose of our competitor price comparison tables is to enable our visitors to compare the prices and purities of products of nominally equivalent quality, for vendors whose price and purity claims are openly available from a website or catalog (without a need for the customer to contact the vendor and request a quotation in order to obtain a price and purity for a standard product size).  However, the quality comparisons in these tables are not absolute:  although our product purities are uniformly very high and are established by careful testing of every lot in our laboratories, we cannot and do not vouch for the purities claimed by other vendors in these competitor price comparison tables.  (We do carry out occasional testing of other vendors’ products — see below —  and we have found many instances where vendors’ product purities are far below their claimed levels.)  Effective April 1, 2011, our price comparison tables only include products where a purity commitment is found together with sizes and prices on at least one page of the vendor’s web site.

2.  Researching and compiling sizes and prices for the >140 competitive vendors and more than 135 products is an expensive and tedious task for our small company.  Thus, we typically only perform revisions of these tables annually or less frequently.  The date and source of each competitive vendor’s prices is indicated at the bottom of this page.  Obviously, we make no guarantee that competitive vendor prices will remain unchanged in the interval between our annual revisions.  We may occasionally update individual competitive vendor information and add new competitive vendors as we locate them.

3.  In some circumstances we permanently remove vendors from our price comparison tables.  Circumstances that lead to permanent removal are: 

    A.  If a vendor does not honor its advertised price (ignoring small price increases) for one or more products when an order is attempted.  Example:  a vendor listed a compound at $808 for 10 grams on its website price list, but quoted $4,500 per 10 grams upon our inquiry.  That vendor has been permanently removed from our price comparison tables.

    B.  If, on a product-by-product basis, our analyses of a vendor’s product show purities more than a few percent below the vendor’s advertised purities.  Example:  a vendor advertises a purity for 97% for a compound, but repeated elemental analyses show a minimum of 9% inorganic material present, beyond any salt counterion.  Another example: an HPLC analysis at a relevant UV detector wavelength shows 92% purity for a vendor’s product for which 98% purity is claimed.  We realize of course that UV absorbances for impurities can be higher than for the main compound, but our products meet our purity commitment of typically >99% irrespective of absorbance factors for impurities, and we apply that standard to products from other vendors.

    C.  Again on a product-by-product basis, if a vendor makes no purity claim for a given product, then their product is not shown on that product table.

    D.  If we find that, as a pattern, several of a competitive vendor’s products fail to meet their advertised purity commitments by significant margins, then that vendor is permanently removed from all of our competitor price tables.

    E.  Finally, if we determine that a vendor has provided fraudulent information on its website, in its literature or on a certificate of analysis, that vendor is permanently removed from all of our price comparison tables.

4.  IMPORTANT:  The presence of a competitive vendor entry in our price comparison tables does not necessarily mean that we have analyzed any of that vendor’s products for purity; indeed, in most cases we have not.

5.  Please note:  where necessary, competitor prices are rounded to the nearest whole dollar.

To the best of our knowledge, the prices listed above for our competitors’ products were correct as of the dates listed below for the sources of our annual price information update process.

Pricing Sources and Dates: LC Labs – Current, Calbiochem – website – Dec 2011, Sigma – website – Jan 2012, Enzo – website – Dec 2011, Tocris – website – Jan 2012, AG Scientific – website – Jan 2012, Axxora – website – Dec 2011, Indofine – website – Dec 2011, LKT Labs – website – Dec 2011, Santa Cruz Biotech – website – Dec 2011, Active Biochem – website – Dec 2011, US Biological – website – Dec 2011, BioVision – website – Dec 2011, Logan Nat. Products – website – Dec 2011, BioShop Canada – website – Dec 2011, GenDepot – website – Dec 2011, R&D Systems – website – Jan 2012, Ascent Scientific – website – Dec 2011, Alfa Aesar – website – Dec 2011, Cambridge BioScience – website – Dec 2011, Selleck Chemicals – website – Dec 2011, StressMarq – website – Dec 2011, Stemgent – website – Dec 2011, JiHe Pharmaceutica – website – Dec 2011, antibodies-online – website – Dec 2011, biorbyt.com – website – Dec 2011, Reagents Direct – website – Dec 2011, biokits.com – website – Dec 2011, HuoLi Biochem – website – Dec 2011, Cellagen Technology – website – Dec 2011, AddexBio – website – Dec 2011, Synthonix – website – Dec 2011.

Note:  Sigma’s product C4116 is the hydrated form of this compound.