标签归档：cleancap

CleanCap Cas9 mRNA开放阅读框序列

发表于由

 

CleanCap Cas9 mRNA开放阅读框序列
TriLink产品：
L-7206（用5-甲氧基-U取代）https://www.trilinkbiotech.com/cleancap-cas9-mrna-5mou.html
L-7606（野生型）https://www.trilinkbiotech.com/cleancap-cas9-mrna.html
mRNA可从TriLink BioTechnologies，LLC购买。有关更多信息，请联系sales@trilinkbiotech.com。
此处包含的序列是TriLink BioTechnologies，LLC的机密信息，仅供客户使用。

1 AUGGCCCCCA AGAAGAAGCG GAAGGUGGGC AUCCACGGCG UGCCCGCCGC CGACAAGAAG UACAGCAUCG GCCUGGACAU CGGCACCAAC AGCGUGGGCU 101 GGGCCGUGAU CACCGACGAG UACAAGGUGC CCAGCAAGAA GUUCAAGGUG CUGGGCAACA CCGACCGGCA CAGCAUCAAG AAGAACCUGA UCGGCGCCCU 201 GCUGUUCGAC AGCGGCGAGA CCGCCGAGGC CACCCGGCUG AAGCGGACCG CCCGGCGGCG GUACACCCGG CGGAAGAACC GGAUCUGCUA CCUGCAGGAG 301 AUCUUCAGCA ACGAGAUGGC CAAGGUGGAC GACAGCUUCU UCCACCGGCU GGAGGAGAGC UUCCUGGUGG AGGAGGACAA GAAGCACGAG CGGCACCCCA 401 UCUUCGGCAA CAUCGUGGAC GAGGUGGCCU ACCACGAGAA GUACCCCACC AUCUACCACC UGCGGAAGAA GCUGGUGGAC AGCACCGACA AGGCCGACCU 501 GCGGCUGAUC UACCUGGCCC UGGCCCACAU GAUCAAGUUC CGGGGCCACU UCCUGAUCGA GGGCGACCUG AACCCCGACA ACAGCGACGU GGACAAGCUG 601 UUCAUCCAGC UGGUGCAGAC CUACAACCAG CUGUUCGAGG AGAACCCCAU CAACGCCAGC GGCGUGGACG CCAAGGCCAU CCUGAGCGCC CGGCUGAGCA 701 AGAGCCGGCG GCUGGAGAAC CUGAUCGCCC AGCUGCCCGG CGAGAAGAAG AACGGCCUGU UCGGCAACCU GAUCGCCCUG AGCCUGGGCC UGACCCCCAA 801 CUUCAAGAGC AACUUCGACC UGGCCGAGGA CGCCAAGCUG CAGCUGAGCA AGGACACCUA CGACGACGAC CUGGACAACC UGCUGGCCCA GAUCGGCGAC 901 CAGUACGCCG ACCUGUUCCU GGCCGCCAAG AACCUGAGCG ACGCCAUCCU GCUGAGCGAC AUCCUGCGGG UGAACACCGA GAUCACCAAG GCCCCCCUGA1001 GCGCCAGCAU GAUCAAGCGG UACGACGAGC ACCACCAGGA CCUGACCCUG CUGAAGGCCC UGGUGCGGCA GCAGCUGCCC GAGAAGUACA AGGAGAUCUU1101 CUUCGACCAG AGCAAGAACG GCUACGCCGG CUACAUCGAC GGCGGCGCCA GCCAGGAGGA GUUCUACAAG UUCAUCAAGC CCAUCCUGGA GAAGAUGGAC1201 GGCACCGAGG AGCUGCUGGU GAAGCUGAAC CGGGAGGACC UGCUGCGGAA GCAGCGGACC UUCGACAACG GCAGCAUCCC CCACCAGAUC CACCUGGGCG1301 AGCUGCACGC CAUCCUGCGG CGGCAGGAGG ACUUCUACCC CUUCCUGAAG GACAACCGGG AGAAGAUCGA GAAGAUCCUG ACCUUCCGGA UCCCCUACUA1401 CGUGGGCCCC CUGGCCCGGG GCAACAGCCG GUUCGCCUGG AUGACCCGGA AGAGCGAGGA GACCAUCACC CCCUGGAACU UCGAGGAGGU GGUGGACAAG1501 GGCGCCAGCG CCCAGAGCUU CAUCGAGCGG AUGACCAACU UCGACAAGAA CCUGCCCAAC GAGAAGGUGC UGCCCAAGCA CAGCCUGCUG UACGAGUACU1601 UCACCGUGUA CAACGAGCUG ACCAAGGUGA AGUACGUGAC CGAGGGCAUG CGGAAGCCCG CCUUCCUGAG CGGCGAGCAG AAGAAGGCCA UCGUGGACCU1701 GCUGUUCAAG ACCAACCGGA AGGUGACCGU GAAGCAGCUG AAGGAGGACU ACUUCAAGAA GAUCGAGUGC UUCGACAGCG UGGAGAUCAG CGGCGUGGAG1801 GACCGGUUCA ACGCCAGCCU GGGCACCUAC CACGACCUGC UGAAGAUCAU CAAGGACAAG GACUUCCUGG ACAACGAGGA GAACGAGGAC AUCCUGGAGG1901 ACAUCGUGCU GACCCUGACC CUGUUCGAGG ACCGGGAGAU GAUCGAGGAG CGGCUGAAGA CCUACGCCCA CCUGUUCGAC GACAAGGUGA UGAAGCAGCU2001 GAAGCGGCGG CGGUACACCG GCUGGGGCCG GCUGAGCCGG AAGCUGAUCA ACGGCAUCCG GGACAAGCAG AGCGGCAAGA CCAUCCUGGA CUUCCUGAAG2101 AGCGACGGCU UCGCCAACCG GAACUUCAUG CAGCUGAUCC ACGACGACAG CCUGACCUUC AAGGAGGACA UCCAGAAGGC CCAGGUGAGC GGCCAGGGCG2201 ACAGCCUGCA CGAGCACAUC GCCAACCUGG CCGGCAGCCC CGCCAUCAAG AAGGGCAUCC UGCAGACCGU GAAGGUGGUG GACGAGCUGG UGAAGGUGAU2301 GGGCCGGCAC AAGCCCGAGA ACAUCGUGAU CGAGAUGGCC CGGGAGAACC AGACCACCCA GAAGGGCCAG AAGAACAGCC GGGAGCGGAU GAAGCGGAUC2401 GAGGAGGGCA UCAAGGAGCU GGGCAGCCAG AUCCUGAAGG AGCACCCCGU GGAGAACACC CAGCUGCAGA ACGAGAAGCU GUACCUGUAC UACCUGCAGA2501 ACGGCCGGGA CAUGUACGUG GACCAGGAGC UGGACAUCAA CCGGCUGAGC GACUACGACG UGGACCACAU CGUGCCCCAG AGCUUCCUGA AGGACGACAG2601 CAUCGACAAC AAGGUGCUGA CCCGGAGCGA CAAGAACCGG GGCAAGAGCG ACAACGUGCC CAGCGAGGAG GUGGUGAAGA AGAUGAAGAA CUACUGGCGG2701 CAGCUGCUGA ACGCCAAGCU GAUCACCCAG CGGAAGUUCG ACAACCUGAC CAAGGCCGAG CGGGGCGGCC UGAGCGAGCU GGACAAGGCC GGCUUCAUCA2801 AGCGGCAGCU GGUGGAGACC CGGCAGAUCA CCAAGCACGU GGCCCAGAUC CUGGACAGCC GGAUGAACAC CAAGUACGAC GAGAACGACA AGCUGAUCCG2901 GGAGGUGAAG GUGAUCACCC UGAAGAGCAA GCUGGUGAGC GACUUCCGGA AGGACUUCCA GUUCUACAAG GUGCGGGAGA UCAACAACUA CCACCACGCC3001 CACGACGCCU ACCUGAACGC CGUGGUGGGC ACCGCCCUGA UCAAGAAGUA CCCCAAGCUG GAGAGCGAGU UCGUGUACGG CGACUACAAG GUGUACGACG3101 UGCGGAAGAU GAUCGCCAAG AGCGAGCAGG AGAUCGGCAA GGCCACCGCC AAGUACUUCU UCUACAGCAA CAUCAUGAAC UUCUUCAAGA CCGAGAUCAC3201 CCUGGCCAAC GGCGAGAUCC GGAAGCGGCC CCUGAUCGAG ACCAACGGCG AGACCGGCGA GAUCGUGUGG GACAAGGGCC GGGACUUCGC CACCGUGCGG3301 AAGGUGCUGA GCAUGCCCCA GGUGAACAUC GUGAAGAAGA CCGAGGUGCA GACCGGCGGC UUCAGCAAGG AGAGCAUCCU GCCCAAGCGG AACAGCGACA3401 AGCUGAUCGC CCGGAAGAAG GACUGGGACC CCAAGAAGUA CGGCGGCUUC GACAGCCCCA CCGUGGCCUA CAGCGUGCUG GUGGUGGCCA AGGUGGAGAA3501 GGGCAAGAGC AAGAAGCUGA AGAGCGUGAA GGAGCUGCUG GGCAUCACCA UCAUGGAGCG GAGCAGCUUC GAGAAGAACC CCAUCGACUU CCUGGAGGCC3601 AAGGGCUACA AGGAGGUGAA GAAGGACCUG AUCAUCAAGC UGCCCAAGUA CAGCCUGUUC GAGCUGGAGA ACGGCCGGAA GCGGAUGCUG GCCAGCGCCG3701 GCGAGCUGCA GAAGGGCAAC GAGCUGGCCC UGCCCAGCAA GUACGUGAAC UUCCUGUACC UGGCCAGCCA CUACGAGAAG CUGAAGGGCA GCCCCGAGGA3801 CAACGAGCAG AAGCAGCUGU UCGUGGAGCA GCACAAGCAC UACCUGGACG AGAUCAUCGA GCAGAUCAGC GAGUUCAGCA AGCGGGUGAU CCUGGCCGAC3901 GCCAACCUGG ACAAGGUGCU GAGCGCCUAC AACAAGCACC GGGACAAGCC CAUCCGGGAG CAGGCCGAGA ACAUCAUCCA CCUGUUCACC CUGACCAACC4001 UGGGCGCCCC CGCCGCCUUC AAGUACUUCG ACACCACCAU CGACCGGAAG CGGUACACCA GCACCAAGGA GGUGCUGGAC GCCACCCUGA UCCACCAGAG4101 CAUCACCGGC CUGUACGAGA CCCGGAUCGA CCUGAGCCAG CUGGGCGGCG ACAGCGGCGG CAAGCGGCCC GCCGCCACCA AGAAGGCCGG CCAGGCCAAG4201 AAGAAGAAGG GCAGCUACCC CUACGACGUG CCCGACUACG CCUGA     ...AUGGCCCCCAAGAAGAAGCGGAAGGUGGGCAUCCACGGCGUGCCCGCCGCCGACAAGAAGUACAGCAUCGGCCUGGACAUCGGCACCAACAGCGUGGGCUGGGCCGUGAUCACCGACGAGUACAAGGUGCCCAGCAAGAAGUUCAAGGUGCUGGGCAACACCGACCGGCACAGCAUCAAGAAGAACCUGAUCGGCGCCCUGCUGUUCGACAGCGGCGAGACCGCCGAGGCCACCCGGCUGAAGCGGACCGCCCGGCGGCGGUACACCCGGCGGAAGAACCGGAUCUGCUACCUGCAGGAGAUCUUCAGCAACGAGAUGGCCAAGGUGGACGACAGCUUCUUCCACCGGCUGGAGGAGAGCUUCCUGGUGGAGGAGGACAAGAAGCACGAGCGGCACCCCAUCUUCGGCAACAUCGUGGACGAGGUGGCCUACCACGAGAAGUACCCCACCAUCUACCACCUGCGGAAGAAGCUGGUGGACAGCACCGACAAGGCCGACCUGCGGCUGAUCUACCUGGCCCUGGCCCACAUGAUCAAGUUCCGGGGCCACUUCCUGAUCGAGGGCGACCUGAACCCCGACAACAGCGACGUGGACAAGCUGUUCAUCCAGCUGGUGCAGACCUACAACCAGCUGUUCGAGGAGAACCCCAUCAACGCCAGCGGCGUGGACGCCAAGGCCAUCCUGAGCGCCCGGCUGAGCAAGAGCCGGCGGCUGGAGAACCUGAUCGCCCAGCUGCCCGGCGAGAAGAAGAACGGCCUGUUCGGCAACCUGAUCGCCCUGAGCCUGGGCCUGACCCCCAACUUCAAGAGCAACUUCGACCUGGCCGAGGACGCCAAGCUGCAGCUGAGCAAGGACACCUACGACGACGACCUGGACAACCUGCUGGCCCAGAUCGGCGACCAGUACGCCGACCUGUUCCUGGCCGCCAAGAACCUGAGCGACGCCAUCCUGCUGAGCGACAUCCUGCGGGUGAACACCGAGAUCACCAAGGCCCCCCUGAGCGCCAGCAUGAUCAAGCGGUACGACGAGCACCACCAGGACCUGACCCUGCUGAAGGCCCUGGUGCGGCAGCAGCUGCCCGAGAAGUACAAGGAGAUCUUCUUCGACCAGAGCAAGAACGGCUACGCCGGCUACAUCGACGGCGGCGCCAGCCAGGAGGAGUUCUACAAGUUCAUCAAGCCCAUCCUGGAGAAGAUGGACGGCACCGAGGAGCUGCUGGUGAAGCUGAACCGGGAGGACCUGCUGCGGAAGCAGCGGACCUUCGACAACGGCAGCAUCCCCCACCAGAUCCACCUGGGCGAGCUGCACGCCAUCCUGCGGCGGCAGGAGGACUUCUACCCCUUCCUGAAGGACAACCGGGAGAAGAUCGAGAAGAUCCUGACCUUCCGGAUCCCCUACUACGUGGGCCCCCUGGCCCGGGGCAACAGCCGGUUCGCCUGGAUGACCCGGAAGAGCGAGGAGACCAUCACCCCCUGGAACUUCGAGGAGGUGGUGGACAAGGGCGCCAGCGCCCAGAGCUUCAUCGAGCGGAUGACCAACUUCGACAAGAACCUGCCCAACGAGAAGGUGCUGCCCAAGCACAGCCUGCUGUACGAGUACUUCACCGUGUACAACGAGCUGACCAAGGUGAAGUACGUGACCGAGGGCAUGCGGAAGCCCGCCUUCCUGAGCGGCGAGCAGAAGAAGGCCAUCGUGGACCUGCUGUUCAAGACCAACCGGAAGGUGACCGUGAAGCAGCUGAAGGAGGACUACUUCAAGAAGAUCGAGUGCUUCGACAGCGUGGAGAUCAGCGGCGUGGAGGACCGGUUCAACGCCAGCCUGGGCACCUACCACGACCUGCUGAAGAUCAUCAAGGACAAGGACUUCCUGGACAACGAGGAGAACGAGGACAUCCUGGAGGACAUCGUGCUGACCCUGACCCUGUUCGAGGACCGGGAGAUGAUCGAGGAGCGGCUGAAGACCUACGCCCACCUGUUCGACGACAAGGUGAUGAAGCAGCUGAAGCGGCGGCGGUACACCGGCUGGGGCCGGCUGAGCCGGAAGCUGAUCAACGGCAUCCGGGACAAGCAGAGCGGCAAGACCAUCCUGGACUUCCUGAAGAGCGACGGCUUCGCCAACCGGAACUUCAUGCAGCUGAUCCACGACGACAGCCUGACCUUCAAGGAGGACAUCCAGAAGGCCCAGGUGAGCGGCCAGGGCGACAGCCUGCACGAGCACAUCGCCAACCUGGCCGGCAGCCCCGCCAUCAAGAAGGGCAUCCUGCAGACCGUGAAGGUGGUGGACGAGCUGGUGAAGGUGAUGGGCCGGCACAAGCCCGAGAACAUCGUGAUCGAGAUGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGAUGAAGCGGAUCGAGGAGGGCAUCAAGGAGCUGGGCAGCCAGAUCCUGAAGGAGCACCCCGUGGAGAACACCCAGCUGCAGAACGAGAAGCUGUACCUGUACUACCUGCAGAACGGCCGGGACAUGUACGUGGACCAGGAGCUGGACAUCAACCGGCUGAGCGACUACGACGUGGACCACAUCGUGCCCCAGAGCUUCCUGAAGGACGACAGCAUCGACAACAAGGUGCUGACCCGGAGCGACAAGAACCGGGGCAAGAGCGACAACGUGCCCAGCGAGGAGGUGGUGAAGAAGAUGAAGAACUACUGGCGGCAGCUGCUGAACGCCAAGCUGAUCACCCAGCGGAAGUUCGACAACCUGACCAAGGCCGAGCGGGGCGGCCUGAGCGAGCUGGACAAGGCCGGCUUCAUCAAGCGGCAGCUGGUGGAGACCCGGCAGAUCACCAAGCACGUGGCCCAGAUCCUGGACAGCCGGAUGAACACCAAGUACGACGAGAACGACAAGCUGAUCCGGGAGGUGAAGGUGAUCACCCUGAAGAGCAAGCUGGUGAGCGACUUCCGGAAGGACUUCCAGUUCUACAAGGUGCGGGAGAUCAACAACUACCACCACGCCCACGACGCCUACCUGAACGCCGUGGUGGGCACCGCCCUGAUCAAGAAGUACCCCAAGCUGGAGAGCGAGUUCGUGUACGGCGACUACAAGGUGUACGACGUGCGGAAGAUGAUCGCCAAGAGCGAGCAGGAGAUCGGCAAGGCCACCGCCAAGUACUUCUUCUACAGCAACAUCAUGAACUUCUUCAAGACCGAGAUCACCCUGGCCAACGGCGAGAUCCGGAAGCGGCCCCUGAUCGAGACCAACGGCGAGACCGGCGAGAUCGUGUGGGACAAGGGCCGGGACUUCGCCACCGUGCGGAAGGUGCUGAGCAUGCCCCAGGUGAACAUCGUGAAGAAGACCGAGGUGCAGACCGGCGGCUUCAGCAAGGAGAGCAUCCUGCCCAAGCGGAACAGCGACAAGCUGAUCGCCCGGAAGAAGGACUGGGACCCCAAGAAGUACGGCGGCUUCGACAGCCCCACCGUGGCCUACAGCGUGCUGGUGGUGGCCAAGGUGGAGAAGGGCAAGAGCAAGAAGCUGAAGAGCGUGAAGGAGCUGCUGGGCAUCACCAUCAUGGAGCGGAGCAGCUUCGAGAAGAACCCCAUCGACUUCCUGGAGGCCAAGGGCUACAAGGAGGUGAAGAAGGACCUGAUCAUCAAGCUGCCCAAGUACAGCCUGUUCGAGCUGGAGAACGGCCGGAAGCGGAUGCUGGCCAGCGCCGGCGAGCUGCAGAAGGGCAACGAGCUGGCCCUGCCCAGCAAGUACGUGAACUUCCUGUACCUGGCCAGCCACUACGAGAAGCUGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCUGUUCGUGGAGCAGCACAAGCACUACCUGGACGAGAUCAUCGAGCAGAUCAGCGAGUUCAGCAAGCGGGUGAUCCUGGCCGACGCCAACCUGGACAAGGUGCUGAGCGCCUACAACAAGCACCGGGACAAGCCCAUCCGGGAGCAGGCCGAGAACAUCAUCCACCUGUUCACCCUGACCAACCUGGGCGCCCCCGCCGCCUUCAAGUACUUCGACACCACCAUCGACCGGAAGCGGUACACCAGCACCAAGGAGGUGCUGGACGCCACCCUGAUCCACCAGAGCAUCACCGGCCUGUACGAGACCCGGAUCGACCUGAGCCAGCUGGGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCUACCCCUACGACGUGCCCGACUACGCCUGA

CleanCap™ Cas9 mRNA

发表于由

trilink 

CleanCap™  Cas9 mRNA

CleanCapTM CRISPR 相关蛋白 9 mRNA

目录号trilink L-7606

CleanCap Cas9 mRNA

Cas9 mRNA 表达一个版本的酿脓链球菌 SF370 Cas9 蛋白 (CRISPR Associated protein 9)。Cas9 作为 CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) 基因组编辑系统的一部分发挥作用。在 CRISPR 系统中，一个 RNA 引导序列靶向目标位点，Cas9 蛋白被用来进行双链 DNA 切割。

Cas9 mRNA 编码具有 N 和 C 末端核定位信号 (NLS) 的 Cas9 蛋白。mRNA 内两个 NLS 信号的掺入

增加了传递到细胞核的频率，从而增加了 DNA 剪切的速度。此外，C 末端 HA 表位标签有助于 Cas9 蛋白的检测、分离和纯化。

L-7606-20 (20µg)

L-7606-100 (100µg)

L-7606-1000 (1 mg)

L-7606-BK（原液量）

1.0 mg/mL，溶于 1 mM 柠檬酸钠 (pH 6.4) mRNA 长度：4521 个核苷酸

储存于-40 °C 或以下

QC 分析

鉴别和纯度琼脂糖凝胶迁移率；通过浓度：±6%；通过

产品详情

使用 CleanCap™，TriLink 专有的共转录加盖方法对该 mRNA 加盖，从而得到天然存在的 Cap 1 结构，具有较高的加盖效率。多聚腺苷酸化，并针对哺乳动物系统进行了优化。它模拟了一个*加工的成熟 mRNA。

 

处理

 

在-40 °C 或更低温度下储存。在冰上解冻并处理 mRNA。*使用后，打开前脉冲旋转，并等分至一次性使用部分。请勿涡旋。仅使用经过认证的无 RNase 试剂和具有适当无 RNase 技术的耗材。建议使用屏障。避免冻融循环。不得与含血清的培养基混合，除非首先与稳定转染试剂络合。

 

在 Broad、MIT、Harvard、Iowa、UTokyo 和 Rockefeller（分别称为“研究机构”，统称为“机构”）和 TriLink BioTechnologies LLC (“TriLink”) 向产品买方（“有限被许可方”）授予的有限许可下，提供 Cas9/CRISPR 产品和/或其中包含的技术（“产品”），向有限被许可方传达不可转让的权利，即在有限被许可方按照以下要求开展的内部研究中使用所购买的产品金额：

 

i. 有限被许可方不得向任何其他人士或实体出售或以其他方式转让产品（包括但不限于包含全部或部分产品的任何材料），或为任何其他人士或实体的利益使用产品履行服务，

 

ii. 有限被许可方应仅使用从公司采购的协议产品的采购金额，并应仅将协议产品和协议产品的组分用于其内部研究，但以下情形除外：(a) 任何人类或临床用途，包括但不限于对人类的任何管理或任何诊断或预后用途；(b) 任何人类生殖系修饰，包括对以下各项进行修改：  人类胚胎或人类生殖细胞的 DNA；(c) 任何体内兽医或牲畜使用；(d) 为人类或动物开发、生产、分销、进口、出口、运输、销售、提供销售、营销、推广或其他专有权利或产品的开发或使用，或作为人类或动物的检测服务、治疗或诊断；(e) 根据 1938 年《联邦食品、药品和化妆品法案》第 505 节（经修订）、1944 年《公共健康服务法案》第 351 节（经修订）或任何后续法律或美国以外辖区的同等法律或法规，提供营养益处并由监管机构监管为药物或生物制品的产品；(f) 任何农业用途，包括但不限于使用或

在烟草产品的种植、生长、生产、出口或生产中的应用；及 (g) 任何与基因驱动有关的使用或应用（下称“领域”），但有限被许可方获得机构单独许可在领域外使用产品，而非用于任何商业目的的情况除外，

 

iii. 有限被许可方应按照所有适用法律法规（包括但不限于适用的人类健康和动物福利法律法规）使用产品，

 

  • 机构不得就知识产权或产品向有限被许可方提供任何类型的保证（法定或暗示），包括但不限于产品质量、条件、描述、适销性、特定用途的适合性、不侵犯知识产权或不存在潜在或其他缺陷，并且在此明确否认所有此类保证。

 

v. 研究机构应明确放弃对通过使用产品获得的结果的任何保证，包括但不限于对不准确、无效或不完整结果的任何索赔，

vi. 机构及其董事、受托人、管理人员、雇员、代理人、教员、关联研究者和学生不承担责任

向有限被许可方，包括但不限于任何使用或利润损失、业务中断或任何间接损害赔偿、附带损害赔偿、特殊损害赔偿或其他类型的间接损害赔偿，无论该等损害赔偿是如何造成的，也无论该等损害赔偿是由合同、侵权行为、严格产品责任还是其他行为引起的，

 

vii. 有限被许可方应赔偿、保护 TriLink、各研究机构及其现任和前任受托人、董事、管理人员、教员、附属研究者、学生、雇员和代理人以及其各自的继任者、继承人和受让人，使其免受因行使根据有限许可向有限被许可方授予的任何权利或违反有限许可而产生或施加的任何索赔、诉讼、调查、诉讼、要求或判决所产生的任何责任、损害、损失或费用（包括但不限于合理的律师费和费用）的损害。

该等有限被许可方的许可，但前提是，在法律不允许的范围内，该等有限被许可方同意，在法律允许的范围内，其（而非受偿方）应负责因行使根据有限许可向有限被许可方授予的任何权利或因有限被许可方违反有限许可而产生的或与之相关的任何责任、损害、损失或支出，以及

 

viii. 产品及其使用可能涉及一项或多项已专有技术或一项或多项未决专有技术申请，或  更多机构和产品的购买并未根据前述专有技术申请中针对产品或其使用、生产或商业化提出的任何要求传达许可，除非有限许可中明确规定。