标签归档：cdb

cobaltbiologics 抗人 CD79b 重组抗体说明书

发表于由

cobaltbiologics 抗人 CD79b 重组抗体说明书

 

用同种型对照兔 IgG 染色的人外周血淋巴细胞的流式细胞术，然后是 Alexa Fluor 647-抗兔 IgG。A) CD4 与同种型对照。B) CD19 与同种型对照。

用人、小鼠、食蟹猴 CD79b 或无 DNA（模拟）转染 HEK293 细胞，然后用抗人 CD79b 兔 IgG 染色，然后用 Alexa Fluor 647 抗兔 IgG 染色。

抗人 CD79b 重组抗体

这种重组抗体与作为 B 细胞受体复合物 (BCR) 一部分的 CD79b 强烈结合，因此使其成为非常有用的 B 细胞标记抗体。提供 3 种格式以增加灵活性。

目录项目：

  • # cobaltbiologics 201601 – 兔 IgG，kappa – 50 µg – 89 美元

  • # cobaltbiologics 201602 – 小鼠 IgG2a，kappa NullFc™ – 50 µg – 89 美元

  • # cobaltbiologics 201603 – 生物素化兔 Fab – 50 µg – 129 美元

  • # 201601-2MG – 兔 IgG，kappa – 2 mg – $2,000

  • # 201602-2MG – 小鼠 IgG2a, kappa NullFc™ – 2 mg – $2,000

 

规格

克隆 XP2238
发现方法 用表达人CD79b的HEK293细胞免疫家兔，对抗原阳性B细胞进行单细胞分选。可变域序列被克隆、测序和亚克隆到包含各种物种和同种型恒定域的表达载体中
可用格式 兔 IgG/kappa；带有 NullFc™ 的小鼠 IgG2a/kappa（与传统的抗小鼠二级试剂兼容）；生物素化兔 Fab，在重链 C 端具有单一定义的生物素和 His 标签
宿主物种 兔子
免疫原 在 HEK293 细胞上重组表达的人 CD79b（登录号 NM_000626）
验证反应性 人，食蟹猴 (Macaca fascicularis)
已验证的应用程序 流式细胞术
专注 0.5 – 1.0 毫克/毫升
生产 哺乳动物 HEK293 细胞中的瞬时转染表达系统
纯化 蛋白 A 亲和层析 (MAb)、固定化金属离子层析 (Fab)
公式 PBS pH 7.2，含 0.08% 叠氮化纳
贮存 4°C 最多可保存 3 个月，用于更长时间的储存分装并储存在 -20°C
目标 CD79b
备用名称 B29、AGM6、IGB
目标描述 CD79b 与 CD79a 异二聚化，成为 B 细胞受体复合物 (BCR) 的一部分。一种 37-39 kD 的 I 型跨膜蛋白 CD79b 在表达表面 Ig 的 B 细胞上表达。
Uniprot P40259

 

 

重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

发表于由

重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

产品说明书

FAQ

COA

已发表文献

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens. Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, FcR gamma or zeta. The only inhibitory member in human and mouse, Fc gamma  RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses.

 

产品性质

别名（Synonyms）

Fc gamma RII; Fc gamma RIIB; CD32; CD32b; CDw32; FCG2; IGFR2; CD32A; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A

Uniprot No.

 P31994-1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RIIB/CD32b Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala46-Pro217.

分子量(Molecular Weight)

The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data：Human Fc gamma RIIB, His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 9.4μM as determined in SPR assay.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

HB220624重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

 

重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

暂无内容

重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

暂无内容

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens. Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, FcR gamma or zeta. The only inhibitory member in human and mouse, Fc gamma  RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses.

 

产品性质

别名（Synonyms）

Fc gamma RII; Fc gamma RIIB; CD32; CD32b; CDw32; FCG2; IGFR2; CD32A; FCG2 ; FcGR; FCGR2; FCGR21; FCGR2A

Uniprot No.

 P31994-1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RIIB/CD32b Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala46-Pro217.

分子量(Molecular Weight)

The protein has a predicted MW of 22.5 kDa. Due to glycosylation, the protein migrates to 32-37 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data：Human Fc gamma RIIB, His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 9.4μM as determined in SPR assay.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

HB220624重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

 

重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

暂无内容

重组人FcγRIIB/CD32b(His-Avi标签)|Recombinant Human Fc gamma RIIB/CD32b

暂无内容

重组人FcγRIIIB/CD16b(NA1)(His标签)|Recombinant Human Fc gamma RIIIB/CD16b

发表于由

重组人FcγRIIIB/CD16b(NA1)(His标签)|Recombinant Human Fc gamma RIIIB/CD16b

产品说明书

FAQ

COA

已发表文献

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes.

 

产品性质

别名（Synonyms）

CD16, FCG3, FCGR3B, FCRIIIB, IGFR3, CD16b (NA2), FCG3B, FCG3, FCGR3, FCR-10

Uniprot No.

AAA35881.1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RIIIB / CD16b (NA1) Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gly17-Ser200.

分子量(Molecular Weight)

Approximately 22 kDa. Due to glycosylation, the protein migrates to 48–55 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIIB (NA1) , His Tag with an affinity constant of 0.117 μM as determined in SPR assay (Biacore T200).

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRIIIB/CD16b(NA1)(His标签)|Recombinant Human Fc gamma RIIIB/CD16b

HB220707

重组人FcγRIIIB/CD16b(NA1)(His标签)|Recombinant Human Fc gamma RIIIB/CD16b

暂无内容

重组人FcγRIIIB/CD16b(NA1)(His标签)|Recombinant Human Fc gamma RIIIB/CD16b

暂无内容

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes.

 

产品性质

别名（Synonyms）

CD16, FCG3, FCGR3B, FCRIIIB, IGFR3, CD16b (NA2), FCG3B, FCG3, FCGR3, FCR-10

Uniprot No.

AAA35881.1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RIIIB / CD16b (NA1) Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gly17-Ser200.

分子量(Molecular Weight)

Approximately 22 kDa. Due to glycosylation, the protein migrates to 48–55 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data: Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIIB (NA1) , His Tag with an affinity constant of 0.117 μM as determined in SPR assay (Biacore T200).

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRIIIB/CD16b(NA1)(His标签)|Recombinant Human Fc gamma RIIIB/CD16b

HB220707

重组人FcγRIIIB/CD16b(NA1)(His标签)|Recombinant Human Fc gamma RIIIB/CD16b

暂无内容

重组人FcγRIIIB/CD16b(NA1)(His标签)|Recombinant Human Fc gamma RIIIB/CD16b

暂无内容

重组人FcγRIIIB/CD16b(NA2)(His-Avi标签)|Recombinant Human Fc gamma RIIIB/CD16b

发表于由

重组人FcγRIIIB/CD16b(NA2)(His-Avi标签)|Recombinant Human Fc gamma RIIIB/CD16b

产品说明书

FAQ

COA

已发表文献

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta  chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology.

 

产品性质

别名（Synonyms）

CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10

Uniprot No.

O75015-1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RIIIB/CD16b (NA2) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Ser200. The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106.The CD16b NA1 form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36,S65, N82, and I106

分子量(Molecular Weight)

The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data：Human Fc gamma RIIIB, His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 1.3μM as determined in SPR assay.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRIIIB/CD16b(NA2)(His-Avi标签)|Recombinant Human Fc gamma RIIIB/CD16b

HB220624

重组人FcγRIIIB/CD16b(NA2)(His-Avi标签)|Recombinant Human Fc gamma RIIIB/CD16b

暂无内容

重组人FcγRIIIB/CD16b(NA2)(His-Avi标签)|Recombinant Human Fc gamma RIIIB/CD16b

暂无内容

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on their genetic organization and molecular structure, three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta  chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular domains of Fc gamma RIIIA and B share 97% amino acid sequence homology.

 

产品性质

别名（Synonyms）

CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10

Uniprot No.

O75015-1

表达区间及表达系统（Source）

Recombinant Human Fc gamma RIIIB/CD16b (NA2) Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly17-Ser200. The CD16b NA1 differ with the CD16b NA2 in AA36, 65, 82, and 106.The CD16b NA1 form carries R36, N65, D82, and V106, while the CD16b NA2 form carries S36,S65, N82, and I106

分子量(Molecular Weight)

The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance）

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC

活性（Activity）

SPR Data：Human Fc gamma RIIIB, His Tag captured on CM5 Chip via anti-His antibody can bind Rituximab, hFc Tag with an affinity constant of 1.3μM as determined in SPR assay.

内毒素（Endotoxin）

< 1.0 EU per 1μg of the protein by the LAL method.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶（Reconstitution）

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输。-20℃至-80℃保存，一年有效期。

复溶后，–20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。

建议第一次使用时分装冻存，避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康，请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途！

 

产品数据

重组人FcγRIIIB/CD16b(NA2)(His-Avi标签)|Recombinant Human Fc gamma RIIIB/CD16b

HB220624

重组人FcγRIIIB/CD16b(NA2)(His-Avi标签)|Recombinant Human Fc gamma RIIIB/CD16b

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重组人FcγRIIIB/CD16b(NA2)(His-Avi标签)|Recombinant Human Fc gamma RIIIB/CD16b

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重组生物素化人CEACAM-8/CD66b蛋白 Recombinant Biotinylated Human CEACAM-8/CD66b Protein,His-Avi Tag

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重组生物素化人CEACAM-8/CD66b蛋白 Recombinant Biotinylated Human CEACAM-8/CD66b Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名（Synonyms）

CD66b; CEACAM8; CGM6; CD67; NCA-95

表达区间及表达系统（Source）

Biotinylated Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln35-Ser319.[Accession | NP_001807.2]

分子量大小（Molecular Weight）

The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

SPR Data:Biotinylated Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 6.13 μM as determined in SPR assay .

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名（Synonyms）

CD66b; CEACAM8; CGM6; CD67; NCA-95

表达区间及表达系统（Source）

Biotinylated Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln35-Ser319.[Accession | NP_001807.2]

分子量大小（Molecular Weight）

The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

内毒素（Endotoxin）

Less than 1EU per μg by the LAL method.

纯度（Purity）

> 95% as determined by SDS-PAGE and HPLC.

活性（Activity）

SPR Data:Biotinylated Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 6.13 μM as determined in SPR assay .

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法（Reconstitution）

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.