重组人Nectin-2/CD112蛋白 Recombinant Human Nectin-2/CD112 Protein

重组人Nectin-2/CD112蛋白 Recombinant Human Nectin-2/CD112 Protein

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

Nectin2;PVRL2;MPH;CD112

表达区间及表达系统(Source)

Human Nectin-2/CD112 Protein is expressed from HEK293 without tag. It contains Gln32-Leu360.[Accession | Q92692-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.54 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Human Nectin-2, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.19μg/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

Nectin2;PVRL2;MPH;CD112

表达区间及表达系统(Source)

Human Nectin-2/CD112 Protein is expressed from HEK293 without tag. It contains Gln32-Leu360.[Accession | Q92692-2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 35.54 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Human Nectin-2, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.19μg/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

MHC II Dextramer – 识别抗原特异性CD4+ T 细胞

immudex MHC II Dextramer® – 识别抗原特异性 CD4+ T 细胞

提高灵敏度以获得可靠结果

通常在抗原呈递细胞表面表达,MHC II 分子对接并启动 CD4+ T 细胞。

鉴于抗原特异性 CD4+ T 细胞在招募和协调其他免疫细胞方面的作用,它们对许多疾病的免疫反应至关重要。使用标准试剂检测和分层通常具有挑战性,通过 Dextramer® 技术的高亲和力和增强的灵敏度揭示抗原特异性 CD4+ T 细胞。

MHC II Dextramer® 试剂在灵活的葡聚糖骨架上具有多种 MHC 肽复合物和荧光团,专为 CD4+ T 细胞反应的高分辨率表征而设计,即使在低丰度细胞和血液等复杂基质中也是如此。

 

MHC II Dextramer® 通过流式细胞术检测血液样本中的 DRB1*0101/EBV 特异性 T 细胞(内部数据)。

细致而精确的研究工具

使用 MHC II Dextramer® 试剂突破流式细胞仪检测的界限。MHC II Dextramer® 试剂专为高亲和力和出色的信噪比而设计,使您能够自信地检测抗原特异性 CD4+ T 细胞。

  • 全面了解 CD4+ T 细胞反应,甚至是低亲和力的反应。

  • 确保一致、可重复和可比较的结果。

  • 扩大您的研究范围。

  • 确保实验的灵活性。

  • 目录号或所需的 MHC 等位基因-肽组合。
         – 查阅我们的可用MHC 等位基因列表 – 使用我们的预测工具
         计算预测的 MHC 肽亲和力

  • 荧光团(PE、APC、FITC 或无)

  • 测试规模(50,150、500、1000 次测试)。一项测试是 10 µl 试剂,足以染色 1–3 x 10^6 淋巴细胞或 1–3 x 10^5 克隆抗原特异性 T 细胞。

Cat. No.

Allele Peptide Antigen Disease/Organism
FA10001 DRB1*0101 PKYVKQNTLKLAT HA Influenza
FA10002  DRB1*0101  PVSKMRMATPLLMQA CLIP Endogenous Ag
FA10003  DRB1*0101  TSLYNLRRGTALA  EBNA1 EBV 
FA10004 DRB1*0101  PEQWMFQGAPPSQGT EBNA3A EBV 
FA10005  DRB1*0101  QEIYMQHTYPISA Tetanus Toxin Tetanus 
FA10006  DRB1*0101  LPLKMLNIPSINVH pp65 CMV 
FB10001  DRB1*0401  PKYVKQNTLKLAT HA Influenza
FB10002 DRB1*0401  PVSKMRMATPLLMQA CLIP Endogenous Ag
FB10012 DRB1*0401  GAGSLQPLALEGSLQKRG Proinsulin Diabetes 
FB10013 DRB1*0401  IAFTSEHSHFSLK  GAD65  Diabetes 
FB10014 DRB1*0401 NFIRMVISNPAAT GAD65  Diabetes 
FD10001 DRB1*1101 PKYVKQNTLKLAT HA Influenza
FD10002  DRB1*1101 PVSKMRMATPLLMQA CLIP Endogenous Ag
FD10020  DRB1*1101 EHPTFTSQYRIQGKL pp65 CMV
FH10002 DPB1*0401  PVSKMRMATPLLMQA CLIP Endogenous Ag
FH10035 DPB1*0401  SLLMWITQCFLPVF  NY-ESO Cancer 
FH10036  DPB1*0401   TQHFVQENYLEY  MAGE-A3  Cancer 
FH10037  DPB1*0401  KKYFAATQFEPLA Oxytocinase  Cancer
FI10187 HLA-DQ2.5 QLQPFPQPELPY α-I, Gliadin Celiac disease
FI10188 HLA-DQ2.5 GMATPLLMQALPMGAL CLIP2 Endogenous Ag

 

Test Size  Price (EUR) Price (USD)
50 tests 1.349* 1,549*
150 tests 2.599* 2,999*
500 tests 6.199* 7,099*
1000 tests 10.799* 12,349*

 

virogen抗CD38抗体说明书

 

virogen抗CD38抗体说明书

 

ANTI-CD38 HEMATOPOIETIC PRECURSORS

 

 

CATALOG #514-A

抗CD38抗体
目录号:514-A
产品描述:抗CD38 Igg2A(单克隆)
特异性:CD38抗原(人)。造血的
前体,胸腺细胞,活化T细胞,浆细胞。
克隆:ICO-20
原产地:鼠标
纯度:蛋白A纯化
缓冲液/成分:1 x PBS,pH7.2;
浓度:1 mg/ml,根据要求提供大量
储存:长期-80˚C
短期:4˚C
应用:免疫沉淀法,酶联免疫吸附法,
稀释酶联免疫吸附试验:1:1000
装运:冷包装

这些产品仅用于实验室研究或进一步制造,不应用于人类治疗或诊断应用。

重组人CLEC4M/CD299蛋白 Recombinant Human CLEC4M/CD299 Protein,His-Flag Tag

重组人CLEC4M/CD299蛋白 Recombinant Human CLEC4M/CD299 Protein,His-Flag Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

DC-SIGNR;L-SIGN;CD299;CLEC4M

表达区间及表达系统(Source)

Human CLEC4M/CD299 Protein is expressed from HEK293 with His tag and Flag tag at the N-terminus. It contains Gln71-Glu399.[Accession | Q9H2X3-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.81 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE,> 90% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

DC-SIGNR;L-SIGN;CD299;CLEC4M

表达区间及表达系统(Source)

Human CLEC4M/CD299 Protein is expressed from HEK293 with His tag and Flag tag at the N-terminus. It contains Gln71-Glu399.[Accession | Q9H2X3-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.81 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE,> 90% as determined by HPLC

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

celldatasci CD501说明书


celldatasci CD501说明书

RNAstorm FFPE RNA Extraction Kit

Catalog No. CD501

从FFPE组织样品中提取RNA的适合解决方案。包括可处理多达50个样品的试剂。

RNAstorm™试剂盒 – 从FFPE样品中提取强大的RNA

福尔马林固定的组织样品是RNA提取的挑战,通常导致可扩增的RNA的低产率和在涉及酶操作的后续步骤中的差的性能,包括逆转录和测序文库制备。

RNAstorm™提取试剂盒采用专有的CAT5™技术,可增强甲醛诱导损伤的去除,并为RNA提供更高的产量和质量,更好的完整性和更高的可扩增性。该技术由Cell Data Sciences研究人员开发,基于斯坦福大学开展的研究,并于2015年在Nature Chemistry上发表。

无论您是进行RNA-seq,qPCR,微阵列还是其他基因表达分析,RNAstorm™试剂盒都是您获得成功的适合机会。

 

 

一个简单方便的工作流程

RNAstorm试剂盒为从FFPE样品中提取高产量和高完整性RNA提供了便利的工作流程。

该试剂盒还可与DNAstorm™试剂盒结合使用,从同一组织切片中获得纯DNA和RNA。

 

 

可扩增RNA的产量更高

使用来自各种组织的FFPE样品的RNAstorm™试剂盒可以看到RNA产量和质量的显着改善(通过可扩增RNA的量来衡量),其年龄范围从1976年到2015年。

NormalLiver 1NormalLiver 2NormalKidneyRenal CellCarcinomaColorectalCancer 1ColorectalCancer 20102030Fold increase in RNA amountRNAstorm™ KitKit Q

通过FFPE组织的定量RT-PCR比较RNA回收率。“Q”代表竞争性商业FFPE提取试剂盒。

更高完整性RNA

NormalLiver 1NormalLiver 2NormalKidneyRenal CellCarcinomaColorectalCancer 1ColorectalCancer 2020406080DV200 (%)RNAstorm™ KitKit Q

对于使用RNAstorm TM试剂盒提取的RNA相对于流行的商业试剂盒,观察到增加的DV 200值。DV 200表示使用Agilent Bioanalyzer RNA 6000纳米试剂盒测量的长度大于200nt的RNA的百分比。

 

使用来自RNAstorm TM试剂盒和试剂盒Q所见的相同样品的RNA获得的BioAnalyzer痕迹的示例性叠加显示如下。

 

 

应用 RNAseq,PCR,qPCR / RT-PCR,微阵列
套件格式 手动(包括20/50旋转柱); 磁珠套件即将推出
DNase处理步骤 包括
输入样本 福尔马林固定样品(石蜡包埋或固定剂)
推荐的输入样本量 1-4节(每节5-10微米)
分离的RNA类型 总RNA(包括miRNA)
隔离时间 50分钟的动手时间
每个套件包括:

旋转柱
蛋白酶
DNase I 
DNase缓冲液
CAT5™试剂
裂解缓冲液
洗涤缓冲液
结合缓冲液
脱石蜡试剂

 

 

经常问的问题

使用RNAstorm™试剂盒获得的RNA中是否存在任何污染的基因组DNA?

基因组DNA的污染是一个很大的问题,因为它可以干扰下游应用。RNAstorm™试剂盒包括优化的DNase消化步骤,可去除污染的基因组DNA,而不会显着影响RNA产量。虽然此步骤是可选的,但强烈建议您这样做。

我可以从FFPE样本中获得多少RNA?

影响获得的RNA总量的大变量是样品本身的质量(即组织的类型和数量,以及分离和保存样品时的护理)。使用RNAstorm™试剂盒,并假设至少合理的样品质量,可以获得大于1微克的量。

使用RNAstorm™试剂盒获得的RNA可以用于RNA-Seq吗?

是。只要RNA具有足够高的质量,就可以获得高质量的文库。对于Illumina测序,建议使用至少30%的DV200,并且应使用提供至少1μgRNA的样品。

如何准备组织?

使用切片机从FFPE样品中获得5-10μm切片。如果可以可靠地切割,则可以使用厚度小于5μm的部分。建议不要使用厚度超过10微米的部分,因为它们可能无法*消化。此外,每次提取应使用不超过5个部分(每个10μM)。使用过多的组织会导致消化不*并降低产量。

我可以使用非石蜡包埋的组织吗?

是的,可以使用未包埋在石蜡中的组织。在这种情况下,我们建议机械研磨相当于推荐部分数量的组织。

我可以使用FFPE核心吗?

是的,可以使用FFPE核心。由于核心不使用切片机进行处理,因此如果观察到不*消化,则推荐样品消化更加困难并且建议进行机械均质化(例如使用钢珠)。

你推荐哪种脱蜡方法?

RNAstorm™试剂盒包括推荐的Deparaffinization Reagent。与其他常用方法(例如二甲苯)不同,脱石蜡试剂是,无毒的,不需要使用通风橱。在我们的测试中,所包含的试剂在去除石蜡和允许纯化高质量核酸方面至少与二甲苯一样有效。

定量从FFPE样品中获得的RNA的适合方法是什么?

源自FFPE的RNA比从新鲜样品获得的RNA更准确地定量。仅知道RNA的量是否足够,以及RNA是否适用于下游应用,这取决于以下因素:

  • 片段大小分布:如果RNA-Seq由<200nt的片段组成,则5μg样品(通过Qubit测量)对RNA-Seq无用。
  • 化学修饰:对于从福尔马林固定的样品中获得的RNA,各种化学加合物和交联(包括碱基修饰,碱基交联和碱 – 蛋白质交联)可使核酸分子不能被酶接近,因此在下游应用中无活性。
  • 污染:纯化过程中使用的细胞碎片,蛋白质,盐和清洁剂可能会影响下游检测。例如,基于UV / Vis的方法如Nanodrop特别容易受到在200-280nm范围内吸收的污染物的影响。
  • 基于荧光的方法如Qubit容易出现明显错误。使用低浓度的DNA或RNA时,基于染料的检测可能不是线性的。还必须注意RNA样品中基因组DNA的污染,因为用于荧光定量的染料并不*特异于FFPE衍生的DNA或RNA。
  • 定量PCR是定量严重受损和修饰的核酸的优选方法。

RIN编号是否应用于确定FFPE衍生RNA的质量?

虽然RIN数可以提供有关样品碎片程度的一般信息,但它不是敏感或可预测的,不足以成为下游性能的有用指标,特别是对于RNA-Seq。通常,FFPE衍生的RNA的RIN数字将在2到3之间。这些样本中的一些将用于RNA-Seq,而其他样本则不会–RIN不会告诉您。

使用Illumina测序RNA-Seq中稍微更好的预测性是DV200,其代表长于200个核苷酸的RNA片段的百分比。DV200也是基于Bioanalyzer数据计算的,但是存在与所有基于生物分析仪的方法相同的缺点,特别是高变异性。

从FFPE样品中提取RNA时我需要知道什么?
  • 避免使用基于有机溶剂的方法(Trizol)
  • 避免使用刺激的离液盐(即胍盐)
  • 避免使用UV和/或Qubit影响下游定量的洗涤剂(例如Triton X-100)
  • 不要依赖RIN来定量FFPE衍生样品的完整性。看看为什么。请改用DV200。
  • 使用从福尔马林中去除化学修饰的试剂盒或方法。不要将温度升至80˚C或更高。即使在此温度下短时间也会显着降低完整性。
  • 警惕Qubit和Nanodrop浓度,因为有可能被有机分子或DNA污染。
  • 使用qPCR定量RNA,并始终仔细查看解链曲线,以确定是否可能发生非特异性扩增。

 

Macs CD133、BDCA-2(CD303)、BDCA-4(CD304)产品代理


Macs CD133、BDCA-2(CD303)、BDCA-4(CD304)产品代理

简要描述:Macs 代理产品

详细介绍

产品咨询

 德国美天旎公司是一个以细胞分选技术为主、拥有多样化产品的生物技术公司。主要开发研制并销售世界上的细胞分选、细胞生物学、相关分子生物学产品和技术,尤其在干细胞分选、DC细胞分选与分析、细胞因子分泌细胞分选与分析、免疫治疗、再生医学方面占有极大的优势。CD133、BDCA-2(CD303)、BDCA-4(CD304)单抗均为其产品。

敬请关注

Missouri S&T cDNA Resource Center


Missouri S&T cDNA Resource Center

简要描述:Missouri S&T cDNA Resource Center,Missouri S&T cDNA Resource Center代理,cdna产品,cdna产品代理,Missouri S&T cDNA Resource Center上海代理,Missouri S&T cDNA Resource Center北京代理

详细介绍

产品咨询

Missouri S&T cDNA Resource Center,Missouri S&T cDNA Resource Center代理,cdna产品,cdna产品代理,上海代理,北京代理

上海金畔生物科技有限公司专业代理,具体产品信息欢迎电询:

是一个非营利机构,专业为科研工作者提供cDNA克隆编码人信号传导蛋白的研究产品和服务。提供了稳定转染的CHO-K1细胞,M1,M2,M3,M4和M5(毒蕈碱受体1-5)在野生型和3xha标记(N-末端)版本。

 

Name: 5-Hydroxytryptamine (serotonin) receptor 1A (HTR1A)
Catalog Number: #HTR01A0000 
Description  Human 5-hydroxytryptamine (serotonin) receptor HTR1A (wild type) cloned into pcDNA3.1+ (Invitrogen) at BamHI (5') and XhoI (3'). The open reading frame was amplified by the PCR fromHuman whole brain cDNA (Clontech). The insert was sequenced. Insert size = 1270 bp.
Name: 5-Hydroxytryptamine (serotonin) receptor 1A (HTR1A) 3xHA-tagged (N-terminus)
Catalog Number: #HTR01ATN00 
Description  N-terminal 3xHA-tagged Human 5-hydroxytryptamine (serotonin) receptor HTR1A (wild type) cloned into pcDNA3.1+ (Invitrogen) at KpnI (5') and XhoI (3'). The open reading frame was derived from HTR01A0000 by PCR. The insert was sequenced. Insert size= 1354bp.
Name: 5-Hydroxytryptamine (serotonin) receptor 1B (HTR1B)
Catalog Number: #HTR01B0000 
Description  5-hydroxytryptamine (serotonin) receptor (HTR1B) (wild type) cloned into pcDNA3.1+ (Invitrogen) at EcoRI (5') and XhoI (3'). The open reading frame was amplified by the PCR from Human Genomic DNA. The insert was sequenced. Insert size= 1173 bp
Name: 5-Hydroxytryptamine (serotonin) receptor 1B (HTR1B) 3xHA-tagged (N-terminus)
Catalog Number: #HTR01BTN00 
Description  N-terminal 3xHA-tagged human 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B) cloned into pcDNA3.1+ (Invitrogen) at KpnI (5') and XhoI (3'). The open reading frame was derived from HTR01B0000 by PCR. The insert was sequenced. Insert size= 1257bp.
Name: 5-Hydroxytryptamine (serotonin) receptor 1D (HTR1D)
Catalog Number: #HTR01D0000 
Description  Human 5-hydroxytryptamine (serotonin) receptor HTR1D (wild type) cloned into pcDNA3.1+ (Invitrogen) at EcoRI (5') and XbaI (3'). The open reading frame was amplified by the PCR from Image clone (4299633). The insert was sequenced. Insert size = 1140 bp.

上海金畔生物科技有限公司

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

产品说明书

FAQ

COA

已发表文献

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities only recognizing IgG aggregates surrounding multivalent antigens. Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta. The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses.

 

产品性质

别名(Synonyms

CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI

Uniprot No.

 P12314-1

表达区间及表达系统(Source

Recombinant Human Fc gamma RI/CD64 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln16-Pro288. 

分子量(Molecular Weight)

The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度(Purity

> 95% as determined by SDS-PAGE and HPLC

活性(Activity

SPR Data: Human Fc gamma RI, His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 11.3nM as determined in SPR assay.

内毒素(Endotoxin

1.0 EU per 1μg of the protein by the LAL method.

制剂(Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶(Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输-20-80存,一年有效期

复溶后,20 -80°C,在未开封状态下保存 3-6 个月复溶后,2-8°C 保存 2-7 天。

建议第一次使用时分装存,避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康,请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途!

 

产品数据

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

HB220624

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

暂无内容

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

暂无内容

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities only recognizing IgG aggregates surrounding multivalent antigens. Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta. The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses.

 

产品性质

别名(Synonyms

CD64; CD64a; Fc gamma RI; FCG1; Fc-gamma RIA; FCGR1; FcgRI; FCRI; FcRIA; FLJ18345; FCGR1A; IGFR1; Fcr; Fcgr; FCE1A; FcERI

Uniprot No.

 P12314-1

表达区间及表达系统(Source

Recombinant Human Fc gamma RI/CD64 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln16-Pro288. 

分子量(Molecular Weight)

The protein has a predicted MW of 33.5 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

外观(Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度(Purity

> 95% as determined by SDS-PAGE and HPLC

活性(Activity

SPR Data: Human Fc gamma RI, His Tag captured on CM5 Chip via Anti-His Antibody can bind Herceptin, hFc Tag with an affinity constant of 11.3nM as determined in SPR assay.

内毒素(Endotoxin

1.0 EU per 1μg of the protein by the LAL method.

制剂(Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

复溶(Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.

 

运输与保存方法

冰袋运输-20-80存,一年有效期

复溶后,20 -80°C,在未开封状态下保存 3-6 个月复溶后,2-8°C 保存 2-7 天。

建议第一次使用时分装存,避免反复冻融。

 

注意事项

1.避免反复冻融。

2.为了您的安全和健康,请穿实验服并戴一次性手套操作。

3.本产品仅作科研用途!

 

产品数据

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

HB220624

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

暂无内容

重组人FcγRI/CD64(His-Avi标签)|Recombinant Human Fc gamma RI/CD64

暂无内容

ACROBiosystems CD8-H52H4-100ug 说明书

世界*实验材料供应商 ACROBiosystems 上海金畔生物为其中国代理, ACROBiosystems 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, ACROBiosystems 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

ACROBiosystems 中国代理, ACROBiosystems 上海代理, ACROBiosystems 北京代理,ACROBiosystems 广东代理, ACROBiosystems 江苏代理ACROBiosystems 湖北代理,ACROBiosystems 天津,ACROBiosystems 黑龙江代理,ACROBiosystems 内蒙古代理,ACROBiosystems 吉林代理,ACROBiosystems 福建代理, ACROBiosystems 江苏代理, ACROBiosystems 浙江代理, ACROBiosystems 四川代理,

 

ACROBiosystems 公司是一个具有范围影响力的生物技术,其运营包括了位于北美、欧洲和亚洲的数家公司, 主要为生命科学研究、生物制药与疫病诊断等行业提供产品、技术服务和临床应用等解决方案,同时致力于在蛋白组学、药物发现和细胞治疗等领域推广面向工业化和临床转化的技术和产品。ACROBiosystems 的业务遍及60多个国家和地区,并通过在中国、英国等世界范围的不同时区设立分支机构以服务于当地客户与经销商

 

Human FCGR3A / CD16a (V176) Protein

人类FCGR3A / CD16a(V176)蛋白

货号

CD8-H52H4-100ug
CD8-H52H4-1mg (500ug x 2)

代名词

FCGR3A,CD16A,FCG3,FCGR3,IGFR3

资源

在ACROBiosystems的人293细胞(HEK293)中产生人FCGR3A / CD16a(V176)蛋白,也称为人FCGR3A / CD16a蛋白(176 Val),Gly17-Gln208(登录号P08637-1)。

预测的N-末端:Gly 17

分子表征

 

该蛋白质在C端携带多组氨酸标签。

该蛋白质具有计算的23.7kDa的MW。由于糖基化,蛋白质在还原(R)条件(SDS-PAGE)下以40-50kDa迁移。

内毒素

LAL方法每μg不足1.0 EU。

纯度

通过还原性SDS-PAGE测定> 95%。

公式

从0.22μm过滤的PBS溶液(pH7.4)中冻干。通常在冻干前加入海藻糖作为保护剂。

定制产品形式或配方。

重建

请参阅分析证书了解具体说明。

为获得*性能,我们强烈建议您遵循CoA提供的重组协议。

存储

对于长期储存,产品应在-20°C或更低的温度下保存在冻干状态。

请避免反复的冻融循环。

储存后没有观察到活性损失:

  1. 4-8 ° C冻干12个月;
  2. -70 ° C在无菌条件下重建3个月。

 

重组人CD20蛋白VLP Human CD20 Protein-VLP

重组人CD20蛋白VLP Human CD20 Protein-VLP

产品说明书

FAQ

COA

已发表文献

产品信息

 

产品名称

产品编号

规格

Recombinant Human CD20 Protein-VLP

95038ES60

100 μg

95038ES80

1 mg

 

性能参数

 

蛋白别名(Synonyms)

MS4A1,CD20,MS4A-1

表达系统(Source)

HEK293 Cells

表达区间(AA sequence)

Accession #P11836: Met1 – Pro297

内毒素(Endotoxin)

< 1EU per μg by the LAL method.

纯度(Purity)

>90% as determined by DLS.

制剂(Formulation)

115mM Gly 4% trehalose

活性(Activity)

ELISA Data 1Immobilized Human CD20 VLP at 5 ug/mL (30 ul/well) can bind obinutuzumab(P214063), The EC50 was approximately 0.006752 ug/ml.

ELISA Data 2Immobilized Human CD20 VLP at 5 ug/mL (30 ul/well) can bind Anti-CD20 / MS4A1 Reference Antibody (divozilimab), The EC50 was approximately 0.01698 ug/ml.

应用(Applications

ELISA, BLI, SPR, Immunization

稀释(dilution)

Dilute with PBS.

 

储存条件

 

The product should be stored at –85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

  1. Please operate with lab coats and disposable gloves,for your safety.       
  2. This product is for research use only.      

   Ver. CN20240115

重组人CD20蛋白VLP Human CD20 Protein-VLP

暂无内容

重组人CD20蛋白VLP Human CD20 Protein-VLP

暂无内容

产品信息

 

产品名称

产品编号

规格

Recombinant Human CD20 Protein-VLP

95038ES60

100 μg

95038ES80

1 mg

 

性能参数

 

蛋白别名(Synonyms)

MS4A1,CD20,MS4A-1

表达系统(Source)

HEK293 Cells

表达区间(AA sequence)

Accession #P11836: Met1 – Pro297

内毒素(Endotoxin)

< 1EU per μg by the LAL method.

纯度(Purity)

>90% as determined by DLS.

制剂(Formulation)

115mM Gly 4% trehalose

活性(Activity)

ELISA Data 1Immobilized Human CD20 VLP at 5 ug/mL (30 ul/well) can bind obinutuzumab(P214063), The EC50 was approximately 0.006752 ug/ml.

ELISA Data 2Immobilized Human CD20 VLP at 5 ug/mL (30 ul/well) can bind Anti-CD20 / MS4A1 Reference Antibody (divozilimab), The EC50 was approximately 0.01698 ug/ml.

应用(Applications

ELISA, BLI, SPR, Immunization

稀释(dilution)

Dilute with PBS.

 

储存条件

 

The product should be stored at –85~-65℃ for 1 year from date of receipt.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

 

  1. Please operate with lab coats and disposable gloves,for your safety.       
  2. This product is for research use only.      

   Ver. CN20240115

重组人CD20蛋白VLP Human CD20 Protein-VLP

暂无内容

重组人CD20蛋白VLP Human CD20 Protein-VLP

暂无内容