AVI Tag Mouse mAb(AVI Tag 小鼠单抗)(AF2954)

AVI Tag Mouse Monoclonal Antibody
AVI Tag Mouse mAb(AVI Tag 小鼠单抗)(AF2954) 一键复制产品信息

产品编号: AF2954

产品包装:50μl
选择包装

50μl

说明书下载

299.00 10

价格: ¥ 299.00

促销价: ¥ 299.00

促销价: ¥ 299.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
AF2954 AVI Tag Mouse Monoclonal Antibody 50μl 299.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Mouse WB, IP, IF, IHC, ICC AVI-tagged protein in all species

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:500-1:2000 1:50 1:50-1:100 1:50-1:100 1:50-1:100

抗体详细信息如下:

About this Antibody
Name AVI Tag Mouse Monoclonal Antibody
Category Mouse Monoclonal Antibody (mAb); Primary antibody
Isotype IgG1
Purificationmethod Affinity purification
Positive samples
Cellular location
Customer validation
About the Immunogen
Immunogen Synthetic AVI tag peptide coupled to KLH
Sequence GLNDIFEAQKIEWHE
Gene ID
SwissProt
Synonyms
Category Polypeptide protein tag
Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. The extremely tight binding between biotin and avidin or streptavidin makes labeling proteins with biotin a useful tool for many applications. BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide, which is also known as AVI-tag, GLNDIFEAQKIEWHE. Oriented on streptavidin-coated surfaces, AVI creates an ideal presentation for molecular binding interactions, which allows following protein fixation, purification and visualization

包装清单:

产品编号 产品名称 包装
AF2954 AVI Tag Mouse Monoclonal Antibody 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份

保存条件:

AVI Tag Mouse Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

请根据抗体的实际用途选择相应的使用方法。
1. Western检测:
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品:

产品编号 产品名称 包装
AF2852 Flag Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2855 Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2858 HA Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2861 HA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2864 Myc Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2867 Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2870 His Tag (C-terminal Specific) Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2873 His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2876 His Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2879 His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2882 GFP Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2885 GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2888 GST Tag Mouse Monoclonal Antibody 50μl/200μl
AF2891 GST Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2894 V5 Tag Mouse Monoclonal Antibody 50μl/200μl
AF2897 V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2900 Glu-Glu Tag Mouse Monoclonal Antibody 50μl
AF2906 DsRed Tag Mouse Monoclonal Antibody 50μl
AF2909 DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2912 MBP Tag Mouse Monoclonal Antibody 50μl
AF2915 MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2918 PA Tag Mouse Monoclonal Antibody 50μl
AF2921 PA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2924 StrepII Tag Mouse Monoclonal Antibody 50μl
AF2927 StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2930 S Tag Mouse Monoclonal Antibody 50μl
AF2933 S Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2936 TEV-His Tag Mouse Monoclonal Antibody 50μl
AF2939 TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2942 Target Tag Mouse Monoclonal Antibody 50μl
AF2945 Target Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2948 TRX Tag Mouse Monoclonal Antibody 50μl
AF2951 TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2954 AVI Tag Mouse Monoclonal Antibody 50μl
AF2957 AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl

AVI Tag Mouse mAb(AVI Tag 小鼠单抗)(AF2954)
AVI Tag Mouse mAb(AVI Tag 小鼠单抗)(AF2954)

物质安全数据单
输入右侧验证码点击下载:

AVI Tag Mouse mAb(AVI Tag 小鼠单抗)(AF2954)

AVI Tag Mouse mAb(AVI Tag 小鼠单抗)(AF2954)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
标签抗体

使用本产品的文献:

Molecular Innovations AVI-HRP 说明书

世界*实验材料供应商 Molecular Innovations上海金畔生物为其中国代理, Molecular Innovations在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Molecular Innovations就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Molecular Innovations中国代理Molecular Innovations上海代理, Molecular Innovations北京代理,Molecular Innovations广东代理, Molecular Innovations江苏代理Molecular Innovations湖北代理,Molecular Innovations天津,Molecular Innovations黑龙江代理,Molecular Innovations内蒙古代理,Molecular Innovations吉林代理,Molecular Innovations福建代理, Molecular Innovations江苏代理, Molecular Innovations浙江代理, Molecular Innovations四川代理,

 

美国Molecular Innovations公司成立于1990年,是一家致力于抗体、纯化蛋白质及ELISA 试剂盒等产品的研发与生产的大型生物技术企业。该公司在凝血酶及溶栓剂研究领域有着*的优势,其提供的产品有纯化蛋白、单/多克隆抗体、ELISA检测试剂盒、抑制剂与荧光底物、层析树脂、血浆制品及组织切片六大产品系列,主要包括纤溶酶原激活物抑制因子1,肾素,肾素原,玻连蛋白,纤维蛋白,纤维连接蛋白,血纤维蛋白溶酶原,血纤维蛋白溶酶,抗凝血酶,多款ELISA试剂盒,抗体和其他研究使用的试剂。高质量的研究产品保证了研究人员检测操作方便快捷,结果、稳定。

Avidin, HRP Labeled

抗生物素蛋白,HRP标记

货号: AVI-HRP 

类别: Secondary Antibodies, ELISA Components

 

产品描述

抗生物素蛋白,HRP标记

辣根过氧化物酶从鸡蛋清中结合纯化的抗生物素蛋白。对生物素,生物素化蛋白或生物素化抗体具有特异性。推荐用于ELISA的起始稀释度为1:1,000,但适当的工作稀释度必须由zui终用户优化。

 

附加信息

资源

蛋白

存储

4 C

运输条件

冷包

呼气

交货12个月

形成

液体

缓冲

0.01M PBS; 0.0005M EDTA; 10%甘露糖; 2%蔗糖; 10%BSA; <0.1%硫柳汞; pH 8.0

防腐剂

硫柳汞

样本大小

0.01毫升

应用

ELISA

 

 

重组人SIRPβ1亚型3蛋白(His-Avi标签) Human SIRP Beta 1 isoform 3 Protein

重组人SIRPβ1亚型3蛋白(His-Avi标签) Human SIRP Beta 1 isoform 3 Protein

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

SIRPB1;SIRP-beta-1 isoform 3;CD172b

表达区间及表达系统(Source)

Human SIRP Beta 1 isoform 3 Proteinis expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu30-Leu371.[Accession | Q5TFQ8-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

SIRPB1;SIRP-beta-1 isoform 3;CD172b

表达区间及表达系统(Source)

Human SIRP Beta 1 isoform 3 Proteinis expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu30-Leu371.[Accession | Q5TFQ8-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

AVI Tag Mouse mAb (HRP Conjugated)(AVI Tag 小鼠单抗)(AF2957)

AVI Tag Mouse Monoclonal Antibody (HRP Conjugated)
AVI Tag Mouse mAb (HRP Conjugated)(AVI Tag 小鼠单抗)(AF2957) 一键复制产品信息

产品编号: AF2957

产品包装:50μl
选择包装

50μl

说明书下载

388.00 10

价格: ¥ 388.00

促销价: ¥ 388.00

促销价: ¥ 388.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
AF2957 AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl 388.00元
来源 用途 交叉反应性 理论分子量 实际分子量
Mouse WB, IHC, ICC AVI-tagged protein in all species

WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.

H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.

配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。

建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

WB IP IF IHC ICC FC ChIP ChIP-seq
1:500-1:2000 1:50-1:100 1:50-1:100

抗体详细信息如下:

About this Antibody
Name AVI Tag Mouse Monoclonal Antibody (HRP Conjugated)
Category Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody
Isotype IgG1
Purificationmethod Affinity purification
Positive samples
Cellular location
Customer validation
About the Immunogen
Immunogen Synthetic AVI tag peptide coupled to KLH
Sequence GLNDIFEAQKIEWHE
Gene ID
SwissProt
Synonyms
Category Polypeptide protein tag
Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. The extremely tight binding between biotin and avidin or streptavidin makes labeling proteins with biotin a useful tool for many applications. BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide, which is also known as AVI-tag, GLNDIFEAQKIEWHE. Oriented on streptavidin-coated surfaces, AVI creates an ideal presentation for molecular binding interactions, which allows following protein fixation, purification and visualization

包装清单:

产品编号 产品名称 包装
AF2957 AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) 50µl
AZ050 Western一抗稀释液 50ml
说明书 1份

保存条件:

AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

注意事项:

如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。

回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。

提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

请根据抗体的实际用途选择相应的使用方法。
1.Western检测:
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗,4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作,无须再孵育二抗。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。

相关产品:

产品编号 产品名称 包装
AF2852 Flag Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2855 Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2858 HA Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2861 HA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2864 Myc Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2867 Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2870 His Tag (C-terminal Specific) Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2873 His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2876 His Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2879 His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2882 GFP Tag Mouse Monoclonal Antibody 50μl/200μl/1ml
AF2885 GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl/1ml
AF2888 GST Tag Mouse Monoclonal Antibody 50μl/200μl
AF2891 GST Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2894 V5 Tag Mouse Monoclonal Antibody 50μl/200μl
AF2897 V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl/200μl
AF2900 Glu-Glu Tag Mouse Monoclonal Antibody 50μl
AF2906 DsRed Tag Mouse Monoclonal Antibody 50μl
AF2909 DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2912 MBP Tag Mouse Monoclonal Antibody 50μl
AF2915 MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2918 PA Tag Mouse Monoclonal Antibody 50μl
AF2921 PA Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2924 StrepII Tag Mouse Monoclonal Antibody 50μl
AF2927 StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2930 S Tag Mouse Monoclonal Antibody 50μl
AF2933 S Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2936 TEV-His Tag Mouse Monoclonal Antibody 50μl
AF2939 TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2942 Target Tag Mouse Monoclonal Antibody 50μl
AF2945 Target Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2948 TRX Tag Mouse Monoclonal Antibody 50μl
AF2951 TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl
AF2954 AVI Tag Mouse Monoclonal Antibody 50μl
AF2957 AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) 50μl

AVI Tag Mouse mAb (HRP Conjugated)(AVI Tag 小鼠单抗)(AF2957)
AVI Tag Mouse mAb (HRP Conjugated)(AVI Tag 小鼠单抗)(AF2957)

物质安全数据单
输入右侧验证码点击下载:

AVI Tag Mouse mAb (HRP Conjugated)(AVI Tag 小鼠单抗)(AF2957)

AVI Tag Mouse mAb (HRP Conjugated)(AVI Tag 小鼠单抗)(AF2957)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
标签抗体
HRP标记一抗

使用本产品的文献: