AVI Tag Mouse mAb(AVI Tag 小鼠单抗)(AF2954)
产品编号 | 产品名称 | 产品包装 | 产品价格 |
AF2954 | AVI Tag Mouse Monoclonal Antibody | 50μl | 299.00元 |
来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
Mouse | WB, IP, IF, IHC, ICC | AVI-tagged protein in all species | – | – |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
1:500-1:2000 | 1:50 | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
About this Antibody | |
Name | AVI Tag Mouse Monoclonal Antibody |
Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
Isotype | IgG1 |
Purificationmethod | Affinity purification |
Positive samples | – |
Cellular location | – |
Customer validation | – |
About the Immunogen | |
Immunogen | Synthetic AVI tag peptide coupled to KLH |
Sequence | GLNDIFEAQKIEWHE |
Gene ID | – |
SwissProt | – |
Synonyms | – |
Category | Polypeptide protein tag |
Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. The extremely tight binding between biotin and avidin or streptavidin makes labeling proteins with biotin a useful tool for many applications. BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide, which is also known as AVI-tag, GLNDIFEAQKIEWHE. Oriented on streptavidin-coated surfaces, AVI creates an ideal presentation for molecular binding interactions, which allows following protein fixation, purification and visualization |
包装清单:
产品编号 | 产品名称 | 包装 |
AF2954 | AVI Tag Mouse Monoclonal Antibody | 50µl |
AZ050 | Western一抗稀释液 | 50ml |
— | 说明书 | 1份 |
保存条件:
AVI Tag Mouse Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
1. Western检测:
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗,4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2. 免疫染色:
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体,使用后注意回收稀释好的一抗,具体操作可以参考如下网页:
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品:
产品编号 | 产品名称 | 包装 |
AF2852 | Flag Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2855 | Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2858 | HA Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2861 | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2864 | Myc Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2867 | Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2870 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2873 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2876 | His Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2879 | His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2882 | GFP Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2885 | GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2888 | GST Tag Mouse Monoclonal Antibody | 50μl/200μl |
AF2891 | GST Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
AF2894 | V5 Tag Mouse Monoclonal Antibody | 50μl/200μl |
AF2897 | V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
AF2900 | Glu-Glu Tag Mouse Monoclonal Antibody | 50μl |
AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50μl |
AF2909 | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2912 | MBP Tag Mouse Monoclonal Antibody | 50μl |
AF2915 | MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2918 | PA Tag Mouse Monoclonal Antibody | 50μl |
AF2921 | PA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50μl |
AF2927 | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2930 | S Tag Mouse Monoclonal Antibody | 50μl |
AF2933 | S Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2936 | TEV-His Tag Mouse Monoclonal Antibody | 50μl |
AF2939 | TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2942 | Target Tag Mouse Monoclonal Antibody | 50μl |
AF2945 | Target Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2948 | TRX Tag Mouse Monoclonal Antibody | 50μl |
AF2951 | TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2954 | AVI Tag Mouse Monoclonal Antibody | 50μl |
AF2957 | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
搜索
Molecular Innovations AVI-HRP 说明书
世界*实验材料供应商 Molecular Innovations上海金畔生物为其中国代理, Molecular Innovations在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Molecular Innovations就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
Molecular Innovations中国代理, Molecular Innovations上海代理, Molecular Innovations北京代理,Molecular Innovations广东代理, Molecular Innovations江苏代理Molecular Innovations湖北代理,Molecular Innovations天津,Molecular Innovations黑龙江代理,Molecular Innovations内蒙古代理,Molecular Innovations吉林代理,Molecular Innovations福建代理, Molecular Innovations江苏代理, Molecular Innovations浙江代理, Molecular Innovations四川代理,
美国Molecular Innovations公司成立于1990年,是一家致力于抗体、纯化蛋白质及ELISA 试剂盒等产品的研发与生产的大型生物技术企业。该公司在凝血酶及溶栓剂研究领域有着*的优势,其提供的产品有纯化蛋白、单/多克隆抗体、ELISA检测试剂盒、抑制剂与荧光底物、层析树脂、血浆制品及组织切片六大产品系列,主要包括纤溶酶原激活物抑制因子1,肾素,肾素原,玻连蛋白,纤维蛋白,纤维连接蛋白,血纤维蛋白溶酶原,血纤维蛋白溶酶,抗凝血酶,多款ELISA试剂盒,抗体和其他研究使用的试剂。高质量的研究产品保证了研究人员检测操作方便快捷,结果、稳定。
Avidin, HRP Labeled
抗生物素蛋白,HRP标记
货号: AVI-HRP
类别: Secondary Antibodies, ELISA Components
产品描述
抗生物素蛋白,HRP标记
辣根过氧化物酶从鸡蛋清中结合纯化的抗生物素蛋白。对生物素,生物素化蛋白或生物素化抗体具有特异性。推荐用于ELISA的起始稀释度为1:1,000,但适当的工作稀释度必须由zui终用户优化。
附加信息
资源 |
蛋白 |
---|---|
存储 |
4 C |
运输条件 |
冷包 |
呼气 |
交货12个月 |
形成 |
液体 |
缓冲 |
0.01M PBS; 0.0005M EDTA; 10%甘露糖; 2%蔗糖; 10%BSA; <0.1%硫柳汞; pH 8.0 |
防腐剂 |
硫柳汞 |
样本大小 |
0.01毫升 |
应用 |
ELISA |
重组人SIRPβ1亚型3蛋白(His-Avi标签) Human SIRP Beta 1 isoform 3 Protein
重组人SIRPβ1亚型3蛋白(His-Avi标签) Human SIRP Beta 1 isoform 3 Protein
产品说明书
FAQ
COA
已发表文献
性能参数
分子别名(Synonyms) |
SIRPB1;SIRP-beta-1 isoform 3;CD172b |
表达区间及表达系统(Source) |
Human SIRP Beta 1 isoform 3 Proteinis expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu30-Leu371.[Accession | Q5TFQ8-1] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by SDS-PAGE and HPLC. |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution) |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
性能参数
分子别名(Synonyms) |
SIRPB1;SIRP-beta-1 isoform 3;CD172b |
表达区间及表达系统(Source) |
Human SIRP Beta 1 isoform 3 Proteinis expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu30-Leu371.[Accession | Q5TFQ8-1] |
分子量大小(Molecular Weight) |
The protein has a predicted MW of 39.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. |
内毒素(Endotoxin) |
Less than 1EU per μg by the LAL method. |
纯度(Purity) |
> 95% as determined by SDS-PAGE and HPLC. |
制剂(Formulation) |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
重构方法(Reconstitution) |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
储存条件
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
注意事项
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
AVI Tag Mouse mAb (HRP Conjugated)(AVI Tag 小鼠单抗)(AF2957)
产品编号 | 产品名称 | 产品包装 | 产品价格 |
AF2957 | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl | 388.00元 |
来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
Mouse | WB, IHC, ICC | AVI-tagged protein in all species | – | – |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
1:500-1:2000 | – | – | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
About this Antibody | |
Name | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) |
Category | Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody |
Isotype | IgG1 |
Purificationmethod | Affinity purification |
Positive samples | – |
Cellular location | – |
Customer validation | – |
About the Immunogen | |
Immunogen | Synthetic AVI tag peptide coupled to KLH |
Sequence | GLNDIFEAQKIEWHE |
Gene ID | – |
SwissProt | – |
Synonyms | – |
Category | Polypeptide protein tag |
Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties. The extremely tight binding between biotin and avidin or streptavidin makes labeling proteins with biotin a useful tool for many applications. BirA is the Escherichia coli biotin ligase that site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide, which is also known as AVI-tag, GLNDIFEAQKIEWHE. Oriented on streptavidin-coated surfaces, AVI creates an ideal presentation for molecular binding interactions, which allows following protein fixation, purification and visualization |
包装清单:
产品编号 | 产品名称 | 包装 |
AF2957 | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50µl |
AZ050 | Western一抗稀释液 | 50ml |
— | 说明书 | 1份 |
保存条件:
AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
1.Western检测:
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时,确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗,4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作,无须再孵育二抗。具体操作可以参考如下网页:
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
相关产品:
产品编号 | 产品名称 | 包装 |
AF2852 | Flag Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2855 | Flag Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2858 | HA Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2861 | HA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2864 | Myc Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2867 | Myc Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2870 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2873 | His Tag (C-terminal Specific) Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2876 | His Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2879 | His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2882 | GFP Tag Mouse Monoclonal Antibody | 50μl/200μl/1ml |
AF2885 | GFP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
AF2888 | GST Tag Mouse Monoclonal Antibody | 50μl/200μl |
AF2891 | GST Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
AF2894 | V5 Tag Mouse Monoclonal Antibody | 50μl/200μl |
AF2897 | V5 Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl |
AF2900 | Glu-Glu Tag Mouse Monoclonal Antibody | 50μl |
AF2906 | DsRed Tag Mouse Monoclonal Antibody | 50μl |
AF2909 | DsRed Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2912 | MBP Tag Mouse Monoclonal Antibody | 50μl |
AF2915 | MBP Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2918 | PA Tag Mouse Monoclonal Antibody | 50μl |
AF2921 | PA Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50μl |
AF2927 | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2930 | S Tag Mouse Monoclonal Antibody | 50μl |
AF2933 | S Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2936 | TEV-His Tag Mouse Monoclonal Antibody | 50μl |
AF2939 | TEV-His Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2942 | Target Tag Mouse Monoclonal Antibody | 50μl |
AF2945 | Target Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2948 | TRX Tag Mouse Monoclonal Antibody | 50μl |
AF2951 | TRX Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
AF2954 | AVI Tag Mouse Monoclonal Antibody | 50μl |
AF2957 | AVI Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
搜索