世界*实验材料供应商 antibodychain 上海金畔生物为其中国代理， antibodychain 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， antibodychain 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

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antibodychain为您的研究提供更多帮助！antibodychain将整个抗体产业聚集在一个平台开辟了*的网络，市场营销和销售机会。antibodychain平台为顾客提供几万种抗体及相关研究产品。包括抗体，裂解，蛋白质，肽等。antibodychain致力为客户提供更的产品与服务。

antibodychain 113004  HyMAX™ Hybridoma Fusion and Cloning Supplement, 20X 大量现货

antibodychain 113004说明书如下：

HyMAX™ Hybridoma Fusion and Cloning Supplement, 20X

Catalog No.: 113004

Alternate Names: Hybridoma supplement, Hybridoma cloning factor, HCF, hybridoma cloning supplement, HCS, hybridoma cloning fusion supplement, HCFS, hybridoma growth supplement, HGS, hybridoma fusion and cloning booster, hybridoma fusion and cloning supplement, HFCS, Cell Culture Hybridoma Media Supplements

Description: HyMax™ Hybridoma Fusion and Growth Supplement is a proprietary blend of conditioned media containing various cytokines, growth factors & hormones. It is formulated to replace feeder cells during new hybridoma development. It can also be used for fast recovery of frozen cells from liquid nitrogen.

Feeder cells are routinely used during plating of cells after fusion. Typically feeder cells provide many unknown growth factors to the newly developing clones. These includes many cytokines which are involved in isotype switching and maintenance of IgG secretion by B-cells. From our many years of experience with hybridoma development we have formulated this conditioned media that support better growth & higher frequency of IgG producing clone development. It is tested for hybridoma colony forming efficiency and maintenance of IgG secretion.

Usage: Add 5mL of HyMax™ 20x to 95mL of your working media currently used for culturing cells to make 5%. Do not use HyMax™ at more than 10%. Continue with culturing cells with this media until cells have grown well for few generations. Once cells have grown well, culture may be weaned off of HyMax by gradually reducing the percentage in steps to 1%, 0.5% and 0%.

Format: Provided as a sterile filtered, tissue culture tested, 20x concentrate ready to use solution. Never frozen before shipping to customer. Each 100mL bottle is enough to make 2L of working media, enough for doing a complete hybridoma development project.

Storage: It is recommended to store at 4°C – 8°C in refrigerator until use within 6 months. It can be made into aliquots and frozen stored at -20°C – 80°C for long term to use within a 12 month period. Avoid repeated freezing and thawing, which may cause activity loss and or show precipitation.

Stability: 6 months when stored at 4°C – 8°C in refrigerator or 12 months when stored frozen. Once thawed use within 30 days or aliquot and store as needed. Do not freeze & thaw repeatedly.

Shipping: It is prepared fresh (within 30 days) and shipped in liquid form on blue ice packs.

Third party independent comparisons of HyMax to other products:

Figure-1 Analysis of cloning factor supplements for production of IgG-producing B cell hybridomas. Splenocytes were fused with SP2/0 cells, cultured for 14 days before analysis of supernatant from the cultures. Analysis was performed via anti-Fc (gamma) ELISA. IgG positive clones were identified as those producing more than 25ng/mL IgG.

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antibodychain代理

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世界*实验材料供应商 antibodychain 上海金畔生物为其中国代理， antibodychain 在一直是行业的*,一直为广大科研客户提供zui为优质的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， antibodychain 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

 antibodychain 中国代理, antibodychain 上海代理， antibodychain 北京代理，antibodychain 广东代理， antibodychain 江苏代理antibodychain 湖北代理,antibodychain 天津,antibodychain 黑龙江代理,antibodychain 内蒙古代理,antibodychain 吉林代理,antibodychain 福建代理, antibodychain 江苏代理, antibodychain 浙江代理, antibodychain 四川代理,

antibodychain为您的研究提供更多帮助！antibodychain将整个抗体产业聚集在一个平台开辟了*的网络，市场营销和销售机会。antibodychain平台为顾客提供几万种抗体及相关研究产品。包括抗体，裂解，蛋白质，肽等。antibodychain致力为客户提供更优质的产品与服务。

部分产品目录：

上海金畔生物科技有限公司
：

*8006

:
:http://www.jinpanbio.com/
 

热烈祝贺上海金畔生物科技有限公司与antibodychain公司达成代理合作协议，antibodychain上海金畔生物科技有限公司为其中国区代理，在这里我们要感谢广大客户对我们的支持,我们将一如既往的为广大新老客户提供antibodychain的产品和服务，欢迎广大新老客户，金畔实验试剂也会继续秉承客户至上的原则，为您的实验研究提供更多产品与服务。

上海金畔生物科技有限公司antibodychain专业代理，具体产品信息欢迎电询：

antibodychain，antibodychain代理，antibodychain上海代理，antibodychain中国，antibodychain北京代理，antibodychain一级代理，antibodychain代理

antibodychain为您的研究提供更多帮助！antibodychain将整个抗体产业聚集在一个平台开辟了*的网络，市场营销和销售机会。antibodychain平台为顾客提供几万种抗体及相关研究产品。包括抗体，裂解，蛋白质，肽等。antibodychain致力为客户提供更的产品与服务。

部分产品信息如下：

上海金畔生物科技有限公司
：

:
:http://www.jinpanbio.com/