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The Developmental Studies Hybridoma Bank
- was created by NIH as a national resource,
- sells at cost (e.g. 1 ml of supernatant costs $40.00, not $200.00 or more),
- is growing at 10% to 20% per year,
- has been given the distribution rights for monoclonals being generated by an NCI initiative in diagnostics, and
- has begun a second bank for microbes (DSHB-Microbe).
AIIB2
| Antigen: Integrin, beta-1 subunit | Hybridoma Cells Available: Yes | |
| Antigen Species: Human | Depositor: Damsky, C.H. | |
| Antigen Sequence: | Depositors Institution: University of California, San Francisco | |
| Antigen Molecular Weight: Predicted: 88kDa (multiple isoforms 87 to 92kDa); Apparent: 110kDa non-reduced gel mobility, 130kDa reduced gel mobility | Human Protein Atlas: Link | |
| Positive Tested Species Reactivity: Bovine, Canine, Human, Mouse, Sheep | Depositors Notes: Blocks cell attachment to fibronectin, collagen-I, collagen-IV, laminin. This antibody stains aldehyde fixed cells and frozen sections of fixed tissues. Does not appear to work for immunoblotting. Binds protein G. | |
| Gene: ITGB1 | Immunogen: Whole JAR choriocarcinoma trophoblastic cell line | |
| Alternate Gene Names: CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA | Immunogen Sequence: Full length protein | |
| Isotype: RIgG1 | Alternate Antibody Name: | |
| Clonality: Monoclonal | Alternate Antigen Name: | |
| Epitope Mapped: Yes | Myeloma Strain: SP 2/0 | |
| Epitope Location or Sequence: NKGEVFNELVGK (a.a. 207-218) | Uniprot ID: P05556 | |
| Host Species: rat | Entrez Gene ID: 3688 | |
| Additional Characterization: Link | Antibody Registry ID: AB_528306 | |
| Additional Information: Like most anti-integrin antibodies, AIIB2 does not blot well. Refer to the cited papers for protocols required for western blotting. | ||
| Recommended Applications: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot | ||