β-Actin Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF0003
产品包装:100μl
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100μl
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β-Actin Mouse mAb(β-Actin小鼠单抗)(AF0003)
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价格: ¥ 446.00
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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF0003 | β-Actin Mouse Monoclonal Antibody | 100μl | 446.00元 |
| 来源 | 用途 | 交叉反应性 | 分子量 |
| Mouse | WB, ICC, IHC, IF | H, M, R | 43KDa |
WB,Western blot;IP,Immunoprecipitation;IF,Immunofluorescence;IHC,Immunohistochemistry;ICC,Immunocytochemistry;FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ELISA | ChIP |
| 1:1000 | – | 1:20 | 1:20 | 1:20 | – | – | – |
抗体详细信息如下:
|
About this Antibody |
|
|
Name |
β-Actin Mouse Monoclonal Antibody |
|
Category |
Monoclonal antibody (mAb); Primary antibody |
|
Isotype |
IgG1 |
|
Purification |
Affinity chromatography |
|
About the Immunogen |
|
|
Immunogen |
synthetic peptide (KLH-coupled) |
|
Gene ID |
60/11461/81822 |
|
SwissProt |
P60709/P60710/P60711 |
|
Synonyms |
BRWS1; PS1TP5BP1 |
|
Category |
Cytoskeletal Signaling |
|
Background |
Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF0003 | β-Actin Mouse Monoclonal Antibody | 100μl |
| AZ100 | Western一抗稀释液 | 100ml |
| — | 说明书 | 1份 |
保存条件：
β-Actin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋
白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体 操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片:
Fig. 1. Western blot analysis on Hela (A), NIH/3T3 (B) and PC12 (C) cell lysatesusing β-actin mousemonoclonal antibody (AF003).
Fig. 2. Immunofluorescent staining of NIH/3T3 cells using β-actin mouse monoclonalantibody (AF0003). Nucleus (blue) were stained with DAPI.
Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse testis tissueusing β-actin mouse monoclonal antibody (AF0003).
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Increased RBM12 expression predicts poor prognosis in hepatocellular carcinoma based on bioinformatics
J Gastrointest Oncol. 2021 Aug;12(4):1905-1926.;doi: 10.21037/jgo-21-390.
139. Yuxin Wang, Yixun Su, Guangdan Yu, Xiaorui Wang, Xiaoying Chen, Bin Yu, Yijun Cheng, Rui Li, Juan C Sáez, Chenju Yi, Lan Xiao, Jianqin Niu
Reduced Oligodendrocyte Precursor Cell Impairs Astrocytic Development in Early Life Stress
Adv Sci (Weinh). 2021 Aug;8(16):e2101181.;doi: 10.1002/advs.202101181.
140. Xiao-Yue Ge, Tian-Tian Zhu, Mao-Zhong Yao, Hong Liu, Qian Wu, Jie Qiao, Wei-Fang Zhang, Chang-Ping Hu
MicroRNA-137 Inhibited Hypoxia-Induced Proliferation of Pulmonary Artery Smooth Muscle Cells by Targeting Calpain-2
Biomed Res Int. 2021 Sep 1;2021:2202888.;doi: 10.1155/2021/2202888. (IF 2.276)
141. Huan Lan, Lu-Ying Zhang, Wen He, Wan-Ying Li, Zhen Zeng, Bo Qian, Chengqiang Wang, Jia-Le Song
Sinapic Acid Alleviated Inflammation-Induced Intestinal Epithelial Barrier Dysfunction in Lipopolysaccharide- (LPS-) Treated Caco-2 Cells
Mediators Inflamm. 2021 Sep 8;2021:5514075.;doi: 10.1155/2021/5514075.
142. Bei Li, Junjian Yu, Peipei Liu, Taohui Zeng, Xueliang Zeng
Astragaloside IV protects cardiomyocytes against hypoxia injury via HIF-1α and the JAK2/STAT3 pathway
Ann Transl Med. 2021 Sep;9(18):1435.;doi: 10.21037/atm-21-4080. (IF 3.297)
143. Suya Zheng, Han Gu, Guangwei Han, Huiling Xu, Zehui Liu, Ying Lu, Fang He
Porcine Reproductive and Respiratory Syndrome Virus nsp11 Antagonizes Broad Antiviral Effects of MCPIP1 by Inducing Interleukin-17 Expression
J Virol. 2021 Oct 27;95(22):e0111921.;doi: 10.1128/JVI.01119-21. (IF 4.501)
144. Baocheng Xie, Zhanwei Zeng, Shiyi Liao, Chenhui Zhou, Longhuo Wu, Daohua Xu
Kaempferol Ameliorates the Inhibitory Activity of Dexamethasone in the Osteogenesis of MC3T3-E1 Cells by JNK and p38-MAPK Pathways
Front Pharmacol. 2021 Oct 5;12:739326.;doi: 10.3389/fphar.2021.739326. (IF 4.225)
145. Yeqing Wang, Shanshan Zhang, Hailai Ni, Yanjie Zhang, Xiaodong Yan, Yue Gao, Beixuan He, Wenzheng Wang, Chong Liu, Meili Guo
Autophagy is involved in the neuroprotective effect of nicotiflorin
J Ethnopharmacol. 2021 Oct 5;278:114279.;doi: 10.1016/j.jep.2021.114279. (IF 3.69)
146. Tian-Tian Zhu, Chao-Nan Zhu, Yue Qiu, Qian-Shuai Li, Xin Yu, Guo-Jie Hao, Ping Song, Jian Xu, Peng Li, Ya-Ling Yin
Tertiary butylhydroquinone alleviated liver steatosis and increased cell survival via β-arrestin-2/PI3K/AKT pathway
Iran J Basic Med Sci. 2021 Oct;24(10):1428-1436.;doi: 10.22038/IJBMS.2021.58156.12924. (IF 2.146)
147. Lin Shen, Chao Li, Fang Chen, Liangfang Shen, Zhanzhan Li, Na Li
CRISPR/Cas9 genome-wide screening identifies LUC7L2 that promotes radioresistance via autophagy in nasopharyngeal carcinoma cells
Cell Death Discov. 2021 Dec 14;7(1):392.;doi: 10.1038/s41420-021-00783-8.
148. Chang Xiong, Zhongfeng Sun, Jinjin Yu, Yaying Lin
Exosome Component 4 Promotes Epithelial Ovarian Cancer Cell Proliferation, Migration, and Invasion via the Wnt Pathway
Front Oncol. 2021 Dec 8;11:797968.;doi: 10.3389/fonc.2021.797968. (IF 4.848)
149. Lu Xia, Yu Liu, Zhiwei Zhang, Yajuan Gong, Tianyi Yu, Dan Zhao, Wen Qiu, Yingwei Wang, Jing Zhang
Modulation of IL-6 Expression by KLF4-Mediated Transactivation and PCAF-Mediated Acetylation in Sublytic C5b-9-Induced Rat Glomerular Mesangial Cells
Front Immunol. 2022 Jan 3;12:779667.;doi: 10.3389/fimmu.2021.779667. (IF 5.085)
150. Hu Zhou, Jingxin Zhang, Huaxiang Shi, Pengfei Li, Xin Sui, Yongan Wang, Liyun Wang
Downregulation of CDK5 signaling in the dorsal striatum alters striatal microcircuits implicating the association of pathologies with circadian behavior in mice
Mol Brain. 2022 Jun 14;15(1):53.;doi: 10.1186/s13041-022-00939-2. (IF 4.686)
151. Xin Ye, Mengyi Li, Wei Bian, Anting Wu, Ting Zhang, Junwei Li, Peng Zhou, Huairui Cui, Yu-Qiang Ding, Min Liao, Chenyou Sun
RBP-J deficiency promoted the proliferation and differentiation of CD133-positive cells in both in vitro and in vivo studies
Eur J Neurosci. 2022 Jul;56(2):3839-3860.;doi: 10.1111/ejn.15727. (IF 3.115)
β-Actin Mouse mAb (HRP Conjugated)(β-Actin 小鼠单抗)(AF2815-1ml)
β-Actin Mouse Monoclonal Antibody (HRP Conjugated)
一键复制产品信息 产品编号: AF2815-1ml
产品包装:1ml
选择包装
50μl 200μl 1ml
数
量
说明书下载
价格: ¥ 4586.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2815-50μl | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 50μl | 388.00元 |
| AF2815-200μl | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 200μl | 1168.00元 |
| AF2815-1ml | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 1ml | 4586.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IHC, ICC | All | 43kDa | 43kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | – | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) |
| Category | Mouse Monoclonal Antibody (mAb); HRP Conjugated antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | HeLa, TE-1, 293T, NIH/3T3, Mouse brain, Mouse testis, C6, Rat brain, Rat lung |
| Cellular location | Cytoplasm, cytoskeleton |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic peptide DDIAALVIDNGSG coupled to KLH |
| Sequence | DDIAALVIDNGSG |
| Gene ID | 60/11461/81822 |
| SwissProt | P60709/P60710/P60711 |
| Synonyms | Act; Actx; beta-a; beta-actin; E430023M04Rik |
| Category | Cytoskeletal Signaling |
| Background | Beta actin also known as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2815-50µl | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2815-200µl | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2815-1ml | β-Actin Mouse Monoclonal Antibody (HRP Conjugated) | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
保存条件：
β-Actin Mouse Monoclonal Antibody (HRP Conjugated) -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的HRP一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的HRP一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤和检测等操作，无须再孵育二抗。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2811 | β-Actin Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2815 | β-Actin Mouse Monoclonal Antibody(HRP Conjugated) | 50μl/200μl/1ml |
| AF2819 | GAPDH Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2823 | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2827 | α-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2831 | α-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2835 | β-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2839 | β-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
内参抗体
HRP标记一抗
使用本产品的文献：
β-Actin Mouse mAb(β-Actin 小鼠单抗)(AF2811-1ml)
β-Actin Mouse Monoclonal Antibody
一键复制产品信息 产品编号: AF2811-1ml
产品包装:1ml
选择包装
50μl 200μl 1ml
数
量
说明书下载
价格: ¥ 3528.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF2811-50μl | β-Actin Mouse Monoclonal Antibody | 50μl | 299.00元 |
| AF2811-200μl | β-Actin Mouse Monoclonal Antibody | 200μl | 899.00元 |
| AF2811-1ml | β-Actin Mouse Monoclonal Antibody | 1ml | 3528.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Mouse | WB, IF, IHC, ICC | All | 43kDa | 43kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay;ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | – | 1:50-1:100 | 1:50-1:100 | 1:50-1:100 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | β-Actin Mouse Monoclonal Antibody |
| Category | Mouse Monoclonal Antibody (mAb); Primary antibody |
| Isotype | IgG1 |
| Purificationmethod | Affinity purification |
| Positive samples | HeLa, TE-1, 293T, NIH/3T3, Mouse brain, Mouse testis, C6, Rat brain, Rat lung |
| Cellular location | Cytoplasm, cytoskeleton |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Synthetic peptide DDIAALVIDNGSG coupled to KLH |
| Sequence | DDIAALVIDNGSG |
| Gene ID | 60/11461/81822 |
| SwissProt | P60709/P60710/P60711 |
| Synonyms | Act; Actx; beta-a; beta-actin; E430023M04Rik |
| Category | Cytoskeletal Signaling |
| Background | Beta actin also known as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β-Actin is generally used as an internal control for experimental error. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF2811-50µl | β-Actin Mouse Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2811-200µl | β-Actin Mouse Monoclonal Antibody | 200µl |
| AZ200 | Western一抗稀释液 | 200ml |
| — | 说明书 | 1份 |
| 产品编号 | 产品名称 | 包装 |
| AF2811-1ml | β-Actin Mouse Monoclonal Antibody | 1ml |
| AZ502 | Western一抗稀释液 | AZ500×2 |
| — | 说明书 | 1份 |
保存条件：
β-Actin Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
请根据抗体的实际用途选择相应的使用方法。
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
1. Western检测：
a. 按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：
http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| AF2811 | β-Actin Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2815 | β-Actin Mouse Monoclonal Antibody(HRP Conjugated) | 50μl/200μl/1ml |
| AF2819 | GAPDH Mouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2823 | GAPDH Mouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2827 | α-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2831 | α-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
| AF2835 | β-TubulinMouse Monoclonal Antibody | 50μl/200μl/1ml |
| AF2839 | β-TubulinMouse Monoclonal Antibody (HRP Conjugated) | 50μl/200μl/1ml |
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
内参抗体
使用本产品的文献：
1. Fangyuan Li, Heng Sun, Jiafeng Ren, Bo Zhang, Xi Hu, Chunyan Fang, Jiyoung Lee, Hongzhou Gu, Daishun Ling
A nuclease-mimetic platinum nanozyme induces concurrent DNA platination and oxidative cleavage to overcome cancer drug resistance
Nat Commun. 2022 Nov 30;13(1):7361.;doi: 10.1038/s41467-022-35022-w. (IF 12.121)
β-Actin Rabbit Monoclonal Antibody(β-Actin 兔单抗)(AF5003)
β-Actin Rabbit Monoclonal Antibody
一键复制产品信息 产品编号: AF5003
产品包装:50μl
选择包装
50μl
数
量
说明书下载
价格: ¥ 456.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF5003 | β-Actin Rabbit Monoclonal Antibody | 50μl | 456.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB, IF, IHC | H, M, R, C, Z, Pg, Oryza sativa | 41kDa | 42kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:1000-1:5000 | – | 1:50-1:200 | 1:50-1:200 | 1:50-1:200 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | β-Actin Rabbit Monoclonal Antibody |
| Category | Rabbit Monoclonal Antibody(mAbAntibody (RabMAb); Primary antibody |
| Isotype | IgG |
| Purification method | Affinity purification |
| Positive samples | NIH/3T3, TE-1, 293T, SW480, Mouse brain, Mouse testis, Mouse lung, Rat brain, HeLa |
| Cellular location | Cytoplasm, cytoskeleton |
| Customer validation | WB (Rat , Clam , pig , Human , Chicken , Mouse , Zebrafish); WB (Human); IF (Mouse); IHC (Mouse); CoIP(Human) |
| About the Immunogen | |
| Immunogen | Recombinant protein of human β-actin |
| Sequence | – |
| Gene ID | 60 |
| SwissProt | P60709 |
| Synonyms | ACTB; BRWS1; PS1TP5BP1; beta actin |
| Category | Cytoskeletal Signaling |
| Background | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF5003 | β-Actin Rabbit Monoclonal Antibody | 50μl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
β-Actin Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：http://www.beyotime.com/support/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。
4.代表性图片：
Fig1. Western blot analysis of extracts of various cell lines, using β-Actin Rabbit Monoclonal Antibody at 1:2000 dilution. Secondary antibody: HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time: 30s.
Fig2. Immunohistochemistry of paraffin-embedded human breast cancer using β-Actin Rabbit Monoclonal Antibody at dilution of 1:50 (20x lens).
Fig3. Immunohistochemistry of paraffin-embedded rat kidney using β-Actin Rabbit Monoclonal Antibody at dilution of 1:50 (20x lens).
Fig4. Immunofluorescence analysis of HeLa cells using β-Actin Rabbit Monoclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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内参抗体
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β-Actin-HRP Rabbit Monoclonal Antibody(β-Actin-HRP 兔单抗)(AF5006)
β-Actin-HRP Rabbit Monoclonal Antibody
一键复制产品信息 产品编号: AF5006
产品包装:50μl
选择包装
50μl
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说明书下载
价格: ¥ 598.00
产品简介
使用说明
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相关产品
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产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF5006 | β-Actin-HRP Rabbit Monoclonal Antibody | 50μl | 598.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB | H, M, R, C, Z, Pg, Oryza sativa | 41kDa | 42kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:1000-1:10000 | – | – | – | – | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | β-Actin-HRP Rabbit Monoclonal Antibody |
| Category | Rabbit Monoclonal Antibody (RabMAb); HRP-conjugated antibody |
| Isotype | IgG |
| Purification method | Affinity purification |
| Positive samples | HeLa, HepG2, MCF7, Mouse liver, Mouse kidney, Mouse lung, Rat liver, Rat kidney, Rat lung |
| Cellular location | Cytoplasm, cytoskeleton |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | Recombinant protein of human β-actin |
| Sequence | – |
| Gene ID | 60 |
| SwissProt | P60709 |
| Synonyms | ACTB; BRWS1; PS1TP5BP1; beta actin |
| Category | Cytoskeletal Signaling |
| Background | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AF5006 | β-Actin-HRP Rabbit Monoclonal Antibody | 50μl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
β-Actin-HRP Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：http://www.beyotime.com/support/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。
4.代表性图片：
Fig1. Western blot analysis of extracts of various cell lines, using β-Actin-HRP (HRP-Conjugated β-actin) Rabbit Monoclonal Antibody at 1:1000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: QuickBlock™ Blocking Buffer (P0231). Detection: BeyoECL Star (P0018A). Exposure time: 3s.
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| P0267 | QuickBlock™免疫组化染色二抗稀释液 | 100ml |
| P0280 | InstantView™ SDS-PAGE蛋白染色及上样缓冲液(5X) | 1ml |
| P0050-P0058 | BeyoGel™ SDS-PAGE预制胶(Tris-Gly, 12孔) | 10块/50块 |
| P0448-P0472 | BeyoGel™ Plus PAGE预制胶(Tris-Gly, 10孔/15孔) | 10块/50块 |
| P0501-P0528 | BeyoGel™ Plus PAGE预制胶(Hepes, 10孔/15孔) | 10块/50块 |
| P0552 | BeyoGel™ Plus SDS-PAGE Hepes电泳液(20X) | 100ml/500ml |
| P0670-P0678 | SDS-PAGE下层胶预混液 | 250ml/500ml |
| P0683 | SDS-PAGE上层胶预混液 | 250ml/500ml |
| P0686-P0695 | SDS-PAGE凝胶超快速配制试剂盒 | 可制30-50块胶 |
| P1005-P1031 | 蛋白酶抑制剂混合物(50X/100X) | 1ml/5ml |
| P1045-P1066 | 蛋白酶磷酸酶抑制剂混合物(50X) | 1ml/2ml/5ml/10ml |
| P1081-P1097 | 磷酸酶抑制剂混合物(50X) | 2ml/10ml |
| P1112/P1113 | 去乙酰化酶抑制剂混合物(100X) | 1ml/5ml |
| R0121 | AllProtect™动物组织核酸、蛋白稳定保存液 | 25ml/100ml |
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1. Wenjie Bao, Huayue Xing, Shiwei Cao, Xin Long, Haifeng Liu, Junwei Ma, Fan Guo, Zimu Deng, Xiaolong Liu
Neutrophils restrain sepsis associated coagulopathy via extracellular vesicles carrying superoxide dismutase 2 in a murine model of lipopolysaccharide induced sepsis
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Phosphosolutions公司Anti-Actin产品代理
Phosphosolutions公司Anti-Actin产品代理
简要描述：Phosphosolutions公司Anti-Actin产品代理
详细介绍
产品咨询
公司概况
背景
基因工程– Phosphosolutions是*代可以完整描绘人体的遗传物质序列的企业。
蛋白质体学项目：Phosphosolutions是第二代试图将所有体内蛋白质表达出来的企业。
PhosphoSolutions公司—第三步我们将超越蛋白质体学 进而 专注于磷蛋白质。
our focus 专业特色
PhosphoSolutions公司专注于蛋白质组学中的一个（10-20%）含量的小部分磷蛋白质。磷蛋白是监管控制组蛋白质的关键,这一部分是被称为phosphosome蛋白质。磷蛋白被认为是在神经系统疾病如老年痴呆症和癌症方面的关键元素，实质上,phosphosome是蛋白质组学作物的精华。
公司目标
简明概述：我们要成为世界上的磷蛋白组的提供者。
方案#1， 特异性磷抗体：首先我们要准备磷蛋白组。在激活或磷酸化状态下磷蛋白组是蛋白质识别研究中的*关键工具。
Antibodies 抗体
特异性磷抗体：Detection and quantitation of changes in the state of phosphorylation of specific proteins is of great utility in the quest to establish the function of a given protein and the consequences of its reversible phosphorylation. Two methods commonly used to measure protein phosphorylation and dephosphorylation in cell preparations employ prelabeling with 32Pi or back phosphorylation. These methods continue to be very effective and have advantages for many test systems, but they do have several practical and theoretical limitations （Nestler and Greengard, 1984）. Based in large part on the successful use of short synthetic peptides to produce epitope-targeted antibodies （Lerner, 1982;Sutcliffe et al., 1983）, an immunochemical approach became an attractive alternative for detecting changes in the state of phosphorylation of specific proteins at a specific site. The use of phosphorylation state-specific antibodies takes advantage of the sensitivity and selectivity afforded by immunochemical methodology, combined with relatively simple preparation and potentially broad applications.
The first report of phosphorylation-dependent antibodies appeared in 1981, when polyclonal antibodies that could detect phosphotyrosine-containing proteins were produced by immunization with benzyl phosphonate conjugated to keyhole limpet hemocyanin （KLH） （Ross et al., 1981）. Shortly thereafter, Nairn and colleagues reported the production of serum antibodies that distinguished between the phospho- and dephospho-forms of G-substrate, a protein localized to cerebellar Purkinje cells and phosphorylated by cGMP-dependent protein kinase （Nairn et al., 1982）. A synthetic heptapeptide, Arg-Lys-Asp-Thr-Pro-Ala-Leu, corresponding to a repeated sequence surrounding two phosphorylated threonyl residues in the intact protein, served as antigen. Rabbit antisera against a peptide-KLH conjugate were specific for the dephospho-form of G-substrate. Phospho-specific antibodies were prepared by immunization of rabbits with the purified phosphoprotein, phosphorylated in vitro to a stoichiometry of 2 mol/mol with cGMP-dependent protein kinase. Despite this initial success, other attempts in our laboratory to produce phospho-specific polyclonal antisera by immunization with the phospho-form of intact proteins were not very successful, probably because of two significant factors. First, many phosphorylated proteins are believed to undergo rapid dephosphorylation during immunization, regardless of the route of injection, leading to the loss of the desired phospho-epitope. Second, holoproteins generally contain multiple immunogenic epitopes; this decreases the probability that colonal dominance for a phospho-specific epitope will be obtained.
Taking a more direct approach utilizing phosphorylated and unphosphorylated forms of synthetic phosphopeptides, we developed a general protocol for the production of phosphorylation state-specific antibodies for substrates with established site（s） of phosphorylation （Czernik et al., 1991））. In early stages of our development of this methodology, phosphopeptides were routinely prepared by enzymatic phosphorylation （Czernik et al., 1991）. Although this approach remains perfectly valid today, the preparation of synthetic phosphopeptides using Fmoc derivatives of phosphoamino acids has become the state-of-the-art （Czernik et al., 1995;Czernik et al., 1996）. Likewise, we have examined the use of both polyclonal and monoclonal techniques for antibody production. Given the high success rate that we and others have obtained with the polyclonal technique, it has become the method of choice, because it is an easier and less costly method for the average laboratory. However, when appropriate, this approach can be readily adapted for monoclonal antibody production.
参考文献
1. Czernik AJ, Girault J-A, Nairn AC, Chen J, Snyder G, Kebabian J, Greengard P （1991） Production of phosphorylation state-specific antibodies. Methods Enzymol 201: 264-283.
2. Czernik AJ, Mathers J, Mische SM （1997） Phosphorylation state-specific antibodies. Neuromethods: Regulatory Protein Modification: Techniques & Protocols 30: 219-250.
3. Czernik AJ, Mathers J, Tsou K, Greengard P, Mische SM （1995） Phosphorylation state-specific antibodies: preparation and applications. Neuroprotocols 6: 56-61.
4. Lerner, R. A. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299, 593-596. 1982.
5. Nairn AC, Detre JA, Casnellie JE, Greengard P （1982） Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein. Nature （Lond ） 299: 734-736.
6. Nestler, E. J. and Greengard, P. Protein Phosphorylation in the Nervous System. Nestler and Greengard. Protein Phosphorylation in the Nervous System. [8], 255-299. 1984. New York, Wiley.
8. Sutcliffe JG, Shinnick TM, Green N, Lerner RA （1983） Antibodies that react with predetermined sites on proteins. Science 219: 660-666.
主营产品清单如下：
| Item: | Anti-Actin |
| Category: | |
| Sub-Category: | |
| SKU/Catalog Number: | 125-ACT |
| Datasheet: | click to view |
| SKU | Price | Formulation | Application | Amount | Qty |
| 125-ACT | $275.00 | ascites fluid | WB, IF, IHC | 100 ul |
Cytoskeleton SiR-Actin Kit说明书
Cytoskeleton，Inc。很高兴为药物筛选，信号转导和细胞骨架研究提供广泛的试剂盒和产品。我们专注于生产纯化蛋白质和易于使用的试剂盒，以研究生物化学和细胞过程。我们的试剂盒可用于少量样品，用于基础研究或小屏幕（Biochem Kits™），也可用于大屏幕的高通量样品（CytoDYNAMIX Screens™）。
除了我们现有的产品，我们还在产品线中为微管，微管蛋白，运动蛋白，小G蛋白效应物，GAP，GEF和其他几种蛋白提供药物筛选服务。
Binding Proteins
结合蛋白质
· 概述
· 关于
· CITATIONS
· 常见问题解答
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测定所有肌动蛋白结合蛋白（ABP）以确保其活性与公布的值相当。测定因产品而异，包括F-肌动蛋白切断测定，F-肌动蛋白激活的ATP水解和肌动蛋白聚合测定。显示了测量WASP VCA结构域蛋白在肌动蛋白聚合中的作用（右）。
Cytoskeleton，Inc。销售一系列纯化的肌动蛋白结合蛋白。所有Cytoskeleton的肌动蛋白结合蛋白都具有高纯度，并经过严格的生物活性测试。细胞骨架还为肌动蛋白结合蛋白研究提供肌动蛋白，抗体和试剂盒。 |
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由Arp2 / 3和WASP VCA结构域刺激的肌动蛋白聚合。试剂盒BK003用于研究Arp2 / 3（Cat。＃RP01）和WASP VCA（Cat。＃VCG03）结构域对肌动蛋白聚合的影响。 |
肌动蛋白与细胞中的多种蛋白质相互作用
*，F-和G-肌动蛋白与细胞中的多种蛋白质相互作用（14）。目前有超过150种已知的肌动蛋白结合蛋白（ABP），其占细胞蛋白的约25％。ABP在肌动蛋白细胞骨架上赋予大量功能多样性，其涉及诸如肌肉收缩，唇形突破，细胞运动，胞质分裂和细胞质流动的过程。研究这些过程如何调节肌动蛋白丝功能和动力学是一个重要且不断增长的研究领域。
可以通过使用生物化学，遗传学和免疫学方法分离和研究肌动蛋白结合蛋白。因为存在超过50种类型的肌动蛋白结合蛋白，所以用于鉴定它们的方法及其功能/活性也是多种多样的。肌动蛋白亲和柱通常用于亲和纯化ABP，无论它们是F-或G-肌动蛋白结合。可以通过利用芘肌动蛋白荧光的聚合增强来进行显示潜在ABP影响肌动蛋白动力学的简单测定（参见测量肌动蛋白聚合物部分）。这种性质的研究有助于鉴定新的肌动蛋白结合蛋白。有几篇值得一读的关于识别和量化新肌动蛋白结合蛋白的可能方法的评论（15,16,17）
问题1：哪种肌动蛋白结合蛋白（ABP）可用作F-肌动蛋白结合对照？
答案1：肌动蛋白结合蛋白α-辅肌动蛋白（Cat。＃AT01）是一种F-肌动蛋白结合和交联蛋白，使其可用作F-肌动蛋白结合研究的对照。它的Kd是1μM，这意味着在该浓度下发生50％结合。因此，我们的目标是使用 2μM的α-肌动蛋白作为结合测定中的阳性对照。在结合反应中，2μM浓度的α-辅肌动蛋白相当于0.23μg/μl。此外，α-辅肌动蛋白对pH敏感，其高亲和力发生在pH 7.0。
问题2：Cytoskeleton的肌球蛋白或重的meromyosin是否具有F-肌动蛋白活力？
答案2：遗憾的是，我们不会出售运动级肌球蛋白或重肌红蛋白用于肌动蛋白运动试验。我们发现质量控制测试非常难以复制，因此我们无法充分确保试剂的质量。因此，肌球蛋白（Cat。＃MY02和MY03）和重型meromyosin蛋白（Cat。＃MH01）不推荐用于F-肌动蛋白运动测定。已经测试过这些试剂的客户在运动方面没有取得很好的成功，因此我们不建议购买样品进行测试。如果需要运动级肌动蛋白或重型meromyosin，我们建议遵循原始方案（Kron和Spudich，1985）并在液氮中冷冻100μl等分试样以确保稳定性。
参考文献： SJ Kron和JA Spudich，1985年。荧光肌动蛋白丝在固定在玻璃表面的肌球蛋白上移动。PNAS，83,6272-6276。
SiR-Actin Kit SiR-肌动蛋白试剂盒
50 nmol SiR-Actin and 1 umol verapamil (Cat. # CY-SC001
SiR-肌动蛋白基于结合F-肌动蛋白的天然产物jasplakinolide。SiR-肌动蛋白是荧光的，细胞可渗透的并且对F-肌动蛋白具有高度特异性。Sir-actin在不需要遗传操作或过表达的情况下对内源性F-肌动蛋白进行染色。它在远红色中的发射使光毒性和样品自发荧光小化。SiR-肌动蛋白与GFP和/或m-cherry荧光蛋白相容。它可以使用标准的Cy5滤波器进行成像。SiR-肌动蛋白可用于活细胞和组织中的宽场，共焦，SIM或STED成像。探针数量允许50 – 200染色实验。*
光学特性
λ 腹肌 652纳米
λ EM 674纳米
ε 大 1.0·10 5 摩尔-1 ·厘米-1
*基于以下条件：0.5-1ml染色溶液/染色实验，0.5-1μM探针浓度。通过减少体积或探针浓度可以进一步增加染色实验的数量。
Q1。什么是STED显微镜以及它是如何工作的？
A1。STED显微镜代表受刺激的发射消耗显微镜。它是一种超分辨率显微镜，它可以捕获具有比传统光学显微镜更高分辨率的图像，传统光学显微镜受光的衍射约束。STED使用2个激光脉冲，一个是激发荧光团的激发脉冲，使其发荧光。被称为STED脉冲的第二脉冲通过在未被激发的中心焦斑周围的区域中的受激发射来去激发荧光团，从而继续发荧光。这是通过将STED脉冲聚焦成环形，即所谓的甜甜圈来实现的，其中中心焦点没有STED激光脉冲，赋予荧光区域高分辨率（图1;参见参考文献1了解更多信息）有关STED显微镜的详细信息）。
图1.人原代成纤维细胞中用SiR-微管蛋白标记的微管的STED显微图像。
Q2。为什么SiR肌动蛋白（或微管蛋白）探针适用于STED显微镜检查？
A2。STED显微镜提供了在体内研究纳米极谱规模的细胞细节的能力。为了利用这种超分辨率显微镜，人们必须能够以高特异性选择使用荧光探针检查的区域。此外，荧光探针必须是明亮的，光稳定的，没有或几乎没有光毒性，被激发并在远红光谱中发射。另外，如果探针用于活细胞成像（从而避免细胞固定时发生的固定伪影），则需要高细胞渗透性。SiR肌动蛋白和微管蛋白探针满足所有这些要求。简而言之，STED和SiR探针的组合允许亚细胞肌动蛋白和微管蛋白/微管结构的的荧光可视化及其在活细胞中的物理表征（参见图2）。2和参考 2）。
图2.用SiR-肌动蛋白染色的培养的大鼠海马神经元的STED图像。底部图像是顶部图像的一部分的特写视图，以清楚地显示具有180nm周期性的肌动蛋白环（条纹）。感谢Elisa D'Este，MPI Biophysical Chemistry，Göttingen。
Q3。这些探头的滤波器组是什么？
A3。使用标准Cy5过滤器可视化SiR肌动蛋白和微管蛋白探针。宜激发为650nm，发射为670nm。我们推荐激发波长为630 + 20 nm，发射波长为680 + 20 nm的滤光片（图3）。
图3. SiR探针的激发（蓝色）和发射（红色）光谱。
Q4。为什么SiR探针与其他荧光团相比具有低背景？
A4。SiR探针在近红外/远红光谱范围内被激发并发光，因此避免使用较短波长，例如通常自发荧光的蓝光和绿光，从而产生更高的背景信号。SiR偶联探针具有两种物理状态：1。非荧光，封闭的关闭状态（螺内酯）和2.开放的，高度荧光的开启状态（两性离子）。探针与其配体靶的结合有利于高度荧光的开放状态，而游离的未结合的探针以闭合的非荧光状态存在（图4）。荧光扩增从未结合状态到结合状态是100倍。这导致高灵敏度的生物传感器，其中大部分荧光仅在结合状态下发生（参见参考文献3和4）。
图4.SiR衍生物存在于荧光两性离子（开放）形式（左结构）和非荧光螺（闭合）形式（右结构）之间的平衡中。
Q5：SiR探针在室温下是否稳定？
A5：是的，探头在室温下稳定几天。但是，它很大程度上取决于探针和溶剂。因此，建议将所有探针或溶液储存在-20°C。
Q6：SiR-肌动蛋白和SiR-微管蛋白对细胞有毒吗？
A6：是的，高于某一阈值，两种探针都显示出对细胞增殖和肌动蛋白或微管动力学改变的一些影响。然而，探针的毒性比其母体药物低几个数量级。在HeLa细胞中，肌动蛋白和微管动力学都不会在低于100 nM的浓度下发生改变。在此浓度下，SiR探针有效地标记微管和F-肌动蛋白，从而捕获高信噪比图像。
问题7：探针是否适用于固定细胞？
A7：SiR-肌动蛋白探针可与PFA固定的细胞一起使用。SiR-肌动蛋白在PFA固定的细胞中标记F-肌动蛋白与鬼笔环肽衍生物一样有效。SiR-微管蛋白仅在乙二醇 – 双 – 琥珀酰亚胺基 – 琥珀酸酯（EGS） – 固定的细胞中标记微管。然而，观察到PFA固定细胞的中心体微管的选择性标记。SiR-肌动蛋白和SiR-微管蛋白不适用于甲醇固定的细胞。
问题8：STORM可以成像SiR探针吗？
A8：否 – 在STORM成像中通常使用的非常高的光强度下，在活细胞上观察到光毒性效应。
Q9：哪些生物和组织被SiR探针染色？
A9：此列表仅描述已报告有效的细胞系，组织或生物。细胞系，组织或生物体的省略并不意味着SiR探针不能与特定细胞，组织或生物体一金畔挥作用。
智人：U2OS，成纤维细胞，HeLa，HUVEC，MCF-10A，HCT-116，A549，红细胞
Mus musculus：C2C12，IA32，骨骼肌，原代心肌细胞，原代卵母细胞
褐家鼠：原代海马神经元，原代皮层神经元，NRK
Cercopithecus aethiops：COS-7
Mesocricetus auratus：BHK
果蝇（Drosophila melanogaster）：Notum上皮细胞，S2
Didelphis marsupialis：OK细胞
Q10。SiR探针在3D细胞培养中起作用吗？
A10：是的，探针能够在3D生长环境中染色细胞。
问题11：SiR荧光团的校正因子CF 260和CF 280是多少？
A11：CF 260 = 0.116，CF 280 = 0.147
参考
1.地狱SW和Wichmann J. 1994. 通过受激发射破坏衍射分辨率极限：受激发射 – 耗尽荧光显微镜。选择。快报。19,780-782。
2. D'Este E.等。2015年STED纳米显微镜揭示了皮质细胞骨架的周期性的活神经元的普及。Cell Rep.10,1246-1251。
3. Lukinavicius G.等。2013年的近红外荧光的细胞蛋白的活细胞超分辨率显微镜。纳特。化学。5,132-139。
4. Lukinavicius G.等。2014. 用于细胞骨架的活细胞成像的荧光探针。自然方法。11,731-733。
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特色论文
“用于细胞骨架活细胞成像的荧光探针” ; G.Lukinavičius，L.Reymond，E。D'Este，A。Masharina，F.Göttfert，H。Ta，A.Güther，M。Fournier，S。Rizzo，H。Waldmann，C。Blaukopf，C。Sommer， DW Gerlich，H.-D。Arndt，SW Hell＆K。Johnsson; Nature Methods 11,731-733,2014。
“STED纳米显微镜揭示了活体神经元中皮质下细胞骨架周期性的普遍存在” ; E. D'Este，D。Kamin，F.Göttfert，A。El-Hady，SW Hell; Cell Reports，第10卷，第8期，1246 – 1251，2015年。
“近红外荧光团用于细胞蛋白的活细胞超分辨率显微镜检查” ; G.Lukinavičius，K。Umezawa，N。Olivier，A。Honigmann，G。Yang，T。Plass，V。Mueller，L。Reymond，IRCorrêaJr，Z。Luo，C。Schultz，EA Lemke，P。Heppenstall ，C。Eggeling，S。Manley＆K。Johnsson; Nature Chemistry 5,132-139,2013。
“动态肌动蛋白丝控制人体红细胞膜的机械行为” ; DS Gokhin，RB Nowak，JA Khoory，A。de la Piedra，IC Ghiran和VM Fowler; 摩尔。生物学。细胞; 2015年2月25日。
“可裂解的溶细胞素 – 神经肽Y生物结合物能够实现特定的药物传递并证明细胞内作用模式” ; VM Ahrens，KB Kostelnik，R。Rennert，D.Böhme，S。Kalkhof，D. Kosel，L。Weber，M。von Bergen和AG Beck-Sickinger; J.控制。发布; 209：170-178,2015。
“红色Si-罗丹明药物结合物能够在GFP细胞中成像” ; E. Kim，KS Yang，RJ Giedt和R. Weissleder; 化学。Commun。，50,4504-4507,2014。
“微管的边缘带在视网膜双极突触末端转运和组织线粒体” ; M. Graffe，D。Zenisek和J. Taraska; J. Gen Physiol。卷。146 No.one：109-117,2015。
应用笔记
“用于活细胞STED显微镜的明亮染料” ; S. Pitsch，I.Köster。
|
货号 |
品名 |
规格 |
品牌 |
|
BK165-S |
Signal-Seeker™ SUMOylation 1 Detection Kit |
(10 assay) |
cytoskeleton |
|
BK165 |
Signal-Seeker™ SUMOylation 1 Detection Kit |
(30 assay) |
cytoskeleton |
|
BK163-S |
Signal-Seeker™ Acetyl-Lysine Detection Kit |
(10 assay) |
cytoskeleton |
|
BK163 |
Signal-Seeker™ Acetyl-Lysine Detection Kit |
(30 assay) |
cytoskeleton |
|
BK160-S |
Signal-Seeker™ Phosphotyrosine Detection Kit |
(10 assay) |
cytoskeleton |
|
BK160 |
Signal-Seeker™ Phosphotyrosine Detection Kit |
(30 assay) |
cytoskeleton |
|
BK162-S |
Signal-Seeker™ SUMOylation 2/3 Detection Kit |
(10 assay) |
cytoskeleton |
|
BK162 |
Signal-Seeker™ SUMOylation 2/3 Detection Kit |
(30 assay) |
cytoskeleton |
|
BK161-S |
Signal-Seeker™ Ubiquitination Detection Kit |
(10 assay) |
cytoskeleton |
|
BK161 |
Signal-Seeker™ Ubiquitination Detection Kit |
(30 assay) |
cytoskeleton |
|
AAC04-Beads |
Acetyl-Lysine Affinity Beads |
ea |
cytoskeleton |
|
APY03-Beads |
Anti-Phosphotyrosine Affinity Beads |
ea |
cytoskeleton |
|
ASM11-Beads |
SUMOylation 1 Affinity Beads |
ea |
cytoskeleton |
|
ASM24-Beads |
SUMOylation 2/3 Affinity Beads |
ea |
cytoskeleton |
|
UBA01-Beads |
Ubiquitination Affinity Beads |
ea |
cytoskeleton |
|
CIG02-Beads |
Acetyl-Lysine Control Beads |
ea |
cytoskeleton |
|
CIG03-Beads |
SUMO1 IP Control Beads |
ea |
cytoskeleton |
|
CIG01-Beads |
IgG Control Beads |
ea |
cytoskeleton |
|
CUB02-Beads |
Ubiquitination Control Beads |
ea |
cytoskeleton |
|
BLR01 |
BlastR™ Rapid Lysate Prep Kit |
ea |
cytoskeleton |
|
BLR02 |
BlastR™ Rapid Filtration Kit |
ea |
cytoskeleton |
|
AAC02 |
Acetyl Lysine Antibody Mouse Monoclonal (7B1) |
ea |
cytoskeleton |
|
AAC03 |
Acetyl Lysine Antibody Mouse Monoclonal (19C4B2.1) |
ea |
cytoskeleton |
|
AAC03-HRP |
Acetyl Lysine-HRP Antibody Mouse Monoclonal (19C4B2.1) |
ea |
cytoskeleton |
|
AAC01 |
Acetyl Lysine Antibody Mouse Monoclonal (3C6.08.20) |
ea |
cytoskeleton |
|
APY03 |
Phosphotyrosine Antibody Mouse Monoclonal (27B10) |
ea |
cytoskeleton |
|
APY03-HRP |
Phosphotyrosine-HRP Antibody Mouse Monoclonal (27B10) |
ea |
cytoskeleton |
|
ASM01 |
SUMO1 Antibody Mouse Monoclonal (Clone 5D8B16) |
ea |
cytoskeleton |
|
ASM24 |
SUMO-2/3 Antibody Mouse Monoclonal (Clone 11G2) |
ea |
cytoskeleton |
|
ASM23 |
SUMO-2/3 Antibody Mouse Monoclonal (Clone 12F3) |
ea |
cytoskeleton |
|
ASM23-HRP |
SUMO-2/3-HRP Antibody Mouse Monoclonal (Clone 12F3) |
ea |
cytoskeleton |
|
AUB01 |
Ubiquitin Antibody Mouse Monoclonal |
ea |
cytoskeleton |
|
AUB01-HRP |
Ubiquitin-HRP Antibody Mouse Monoclonal |
ea |
cytoskeleton |
|
PHDG1 |
Acti-stain 488 phalloidin |
ea |
cytoskeleton |
|
PHDH1 |
Acti-stain 555 phalloidin |
ea |
cytoskeleton |
|
PHDN1 |
Acti-stain 670 phalloidin |
ea |
cytoskeleton |
|
BK001 |
Actin Binding Protein Spin-Down Assay Biochem Kit: rabbit skeletal muscle actin |
ea |
cytoskeleton |
|
BK003 |
Actin Polymerization Biochem Kit (fluorescence format): rabbit skeletal muscle actin |
ea |
cytoskeleton |
|
AKL95 |
Actin protein (>95% pure): rabbit skeletal muscle |
ea |
cytoskeleton |
|
AD99 |
Actin protein (>99% pure): bovine cardiac muscle |
ea |
cytoskeleton |
|
AS99 |
Actin protein (>99% pure): chicken gizzard muscle |
ea |
cytoskeleton |
|
AKL99 |
Actin protein (>99% pure): rabbit skeletal muscle |
ea |
cytoskeleton |
|
AKF99 |
Actin protein (pre-formed filaments): rabbit skeletal muscle |
ea |
cytoskeleton |
|
AP05 |
Actin protein (pyrene labeled): rabbit skeletal muscle |
ea |
cytoskeleton |
|
APHR |
Actin protein (rhodamine): human platelet |
ea |
cytoskeleton |
|
AR05 |
Actin protein (rhodamine): rabbit skeletal muscle |
ea |
cytoskeleton |
|
AT01 |
Alpha-actinin protein: rabbit skeletal muscle |
ea |
cytoskeleton |
|
RP01P |
Arp2/3 protein complex: porcine brain |
ea |
cytoskeleton |
|
CS-TFC01 |
Cardiac Thin Filament Complex (CTFC) |
ea |
cytoskeleton |
|
CF01 |
Cofilin 1 protein: human recombinant |
ea |
cytoskeleton |
|
HPG6 |
Gelsolin protein: Homo sapiens recombinant |
ea |
cytoskeleton |
|
MH01 |
Heavy meromyosin protein |
ea |
cytoskeleton |
|
CS-MH03 |
Heavy Meromyosin Protein (HMM Fragment) |
ea |
cytoskeleton |
|
CS-MYS03 |
Myosin – cardiac S1 fragment |
ea |
cytoskeleton |
|
CS-MYS04 |
Myosin – skeletal muscle S1 fragment |
ea |
cytoskeleton |
|
CS-MYS05 |
Myosin – smooth muscle S1 fragment |
ea |
cytoskeleton |
|
MY02 |
Myosin II protein: rabbit skeletal muscle |
ea |
cytoskeleton |
|
MY03 |
Myosin protein: bovine cardiac muscle |
ea |
cytoskeleton |
|
PR02 |
Profilin 1 protein: Untagged, human recombinant |
ea |
cytoskeleton |
|
PHDR1 |
Rhodamine Phalloidin |
ea |
cytoskeleton |
|
CY-SC001 |
SiR-Actin Kit |
ea |
cytoskeleton |
|
CY-SC013 |
SiR700-Actin Kit |
ea |
cytoskeleton |
|
VCG03 |
WASP protein VCA domain: GST tagged: human |
ea |
cytoskeleton |
上海金畔生物科技有限公司是实验试剂一站式采购服务商
1：强大的进口辐射能力，血清、抗体、耗材、大部分限制进口品等。
2：产品种类齐全，经营超过700多品牌，基本涵盖所有生物实验试剂耗材。
3：提供加急服务，货品一般1-2周到货。
4：富有竞争力的价格优势，绝大部分价格有优势。
5：多年积累良好的信誉，大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校，ROCHE，阿斯利康、国药、fisher等知药企。
6：我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等几十家国外公司代理。
7：我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批发，欢迎合作。
Actin抗体(smooth muscle specific,小鼠单抗)(AA132)
Actin抗体(smooth muscle specific,小鼠单抗)
一键复制产品信息 产品编号: AA132
产品包装:>20次
选择包装
>20次
数
量
说明书下载
价格: ¥ 558.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AA132 | Actin抗体(smooth muscle specific,小鼠单抗) | >20次 | 558.00元 |
| 来源 | 用途 | 可检测样品 | 抗体类型 | α-actin分子量 |
| Mouse | WB, IF, IHC, ELISA | Mam, C, S, Fr | IgG2a | ～42kD |
WB, Western blot; IF, Immunofluorescence; IHC, Immunohistochemistry.
Mam, mammalian, including human, mouse, rat, bovine, sheep, pig, rabbit, dog, guinea pig; C, chicken; S, snake; Fr, frog.
本Actin抗体特异性识别α-smooth muscle actin，为进口分装，用人工合成的α-smooth muscle actin N-terminal一段十肽作为抗原制备而成的抗α-smooth muscle actin小鼠单克隆抗体。克隆号为1A4。本Actin抗体(smooth muscle specific)在Western blot或免疫染色时仅识别α-smooth muscle actin。
Actin即肌动蛋白，是细胞的一种重要骨架蛋白。同时Actin在细胞分泌、吞噬、locomotion、cytoplasmic streaming和cytokinesis等过程中起重要作用。Actin在不同物种之间高度保守，以至于很难获得较好的针对actin的抗血清。Actin大致可分为六种，其中四种是不同肌肉组织特异性的，包括α-skeletal muscle actin，α-cardiac muscle actin，α-smooth muscle actin，和γ-smooth muscle actin；其余两种广泛分布于各种组织中，包括β-actin(β-non-muscle)和γ-non-muscle actin。不同的actin之间同源性大于90%，但在N-terminal同源性仅50-60%，因此N-terminal常被用作actin的抗原。
α-smooth muscle actin的免疫染色可以用于判断是否是smooth muscle。
配套提供了Western一抗稀释液，可以用于Western检测时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IF | IHC | ELISA |
| 1:1000 | 1:400 | 1:400 | 1:1000-5000 |
本抗体如果用于常规的Western检测，至少可以检测20次。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AA132-1 | Actin抗体(smooth muscle specific, 小鼠单抗) | 20μl |
| AA132-2 | Western一抗稀释液 | 20ml |
| － | 说明书 | 1份 |
保存条件：
Actin抗体(smooth muscle specific)-20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
在Western实验后，请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10次。稀释后的抗体，包括已经使用过的稀释抗体，4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. Western检测：
a. 按照1:1000用碧云天提供的Western一抗稀释液稀释抗体。
b. 把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动1-2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c. 回收稀释的一抗，4℃保存以备下次继续使用。
d. 按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页： http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
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内参抗体
使用本产品的文献：
1. Wang X, Huang N, Yang M, Wei D, Tai H, Han X, Gong H, Zhou J, Qin J, Wei X, Chen H, Fang T, Xiao H.
FTO is required for myogenesis by positively regulating mTOR-PGC-1α pathway-mediatedmitochondria biogenesis.
Cell Death Dis. 2017 Mar 23;8(3):e2702. (IF 6.304)
2. Wang WY, Zhang J, Wu WY, Li J, Ma YL, Chen WH, Yan H, Wang K, Xu WW, Shen JH, Wang YP.
Inhibition of lipoprotein-associated phospholipase A2 ameliorates inflammation and decreasesatherosclerotic plaque formation in ApoE-deficient mice.
PLoS One. 2011;6(8):e23425. (IF 2.74)
3. Wu X, Liu BJ, Ji S, Wu JF, Xu CQ, Du YJ, You XF, Li B, Le JJ, Xu HL, Duan XH, Dong JC.
Social defeat stress promotes tumor growth and angiogenesis by upregulating vascular endothelial growth factor/extracellular signal-regulated kinase/matrix metalloproteinase signaling in a mouse model of lung carcinoma.
Mol Med Rep. 2015 Jul;12(1):1405-12. (IF 2.1)
4. Li Y, Pu G, Chen C, Yang L.
Inhibition of FHL1 inhibits cigarette smoke extract-induced proliferation in pulmonary arterial smooth muscle cells.
Mol Med Rep. 2015 Sep;12(3):3801-8. (IF 2.1)
5. Guang Jun Chen, Yi Heng Chen, Xia Qing Yang, Zhi Jie Li.
Nano-microcapsule basic fibroblast growth factor combined with hypoxia-inducible factor-1 improves random skin flap survival in rats..
Molecular medicine. 2016 Feb;13(2):1661-6. (IF 2.991)
6. Fang Zhou, Aiguo Dai, Yongliang Jiang, Xiaowu Tan, Xiufeng Zhang.
SENP 1 enhances hypoxia induced proliferation of rat pulmonary artery smooth muscle cells by regulating hypoxia inducible factor 1α.
Molecular medicine. 2016 Mar;13(4):3482-90. (IF 2.991)
7. Sun Q, Wu Y, Zhao F, Wang J.
Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts.
Mol Med Rep. 2017 Aug;16(2):1523-1529. (IF 2.1)
8. Jiang Y, Zhou Y, Peng G, Liu N, Tian H, Pan D, Liu L, Yang X, Li C, Li W, Chen L, Ran P, Dai A
Topotecan prevents hypoxia-induced pulmonary arterial hypertension and inhibits hypoxia-inducible factor-1α and TRPC channels.
INT J BIOCHEM CELL B. 2018 Nov;104:161-170. (IF 3.673)
9. Zhang L, Li B, Zhang B, Zhang H, Suo J
miR-361 enhances sensitivity to 5-fluorouracil by targeting the FOXM1-ABCC5/10 signaling pathway in colorectal cancer.
Oncol Lett. 2019 Oct;18(4):4064-4073. (IF 2.311)
10. Min Ma, Xia-Hui Lin, Hua-Hua Liu, Rui Zhang, Rong-Xin Chen
Suppression of DRP1‑mediated mitophagy increases the apoptosis of hepatocellular carcinoma cells in the setting of chemotherapy
Oncol Rep. 2020 Mar;43(3):1010-1018.;doi: 10.3892/or.2020.7476. (IF 3.417)
11. Yunhe Lu, Liangdong Li, Lei Chen, Yang Gao, Xin Chen, Yiqun Cao
TRIB3 confers glioma cell stemness via interacting with β-catenin
Environ Toxicol. 2020 Jun;35(6):697-706.;doi: 10.1002/tox.22905. (IF 3.118)
12. Xinnong Liu, Chengjia Qu, Yongbao Zhang, Jie Fang, Lequn Teng, Rujiao Zhang, Xiangyu Zhang, Chenyang Shen
Chemokine-like factor 1 (CKLF1) aggravates neointimal hyperplasia through activating the NF-κB /VCAM-1 pathway
FEBS Open Bio. 2020 Sep;10(9):1880-1890.;doi: 10.1002/2211-5463.12942. (IF 2.231)
13. Chaowei Hu, Jing Li, Yunhui Du, Juan Li, Yunyun Yang, Yifan Jia, Lu Peng, Yanwen Qin, Yongxiang Wei
Impact of chronic intermittent hypoxia on the long non-coding RNA and mRNA expression profiles in myocardial infarction
J Cell Mol Med. 2021 Jan;25(1):421-433.;doi: 10.1111/jcmm.16097. (IF 4.486)
14. Lingang Xu, Nan Sun, Guangshuai Li, Linbo Liu
LncRNA H19 promotes keloid formation through targeting the miR-769-5p/EIF3A pathway
Mol Cell Biochem. 2021 Mar;476(3):1477-1487.;doi: 10.1007/s11010-020-04024-x. (IF 2.795)
15. Feng-Ying Gao, Ji-Cai Pang, Miao Wang, Mai-Xin Lu, Zhi-Gang Liu, Jian-Meng Cao, Xiao-Li Ke, Meng-Meng Yi
Structurally diverse genes encode TLR13 in Nile tilapia: The two receptors can recognize Streptococcus 23S RNA and conduct signal transduction through MyD88
Mol Immunol. 2021 Apr;132:60-78.;doi: 10.1016/j.molimm.2021.01.020. (IF 3.641)
Pan-Actin Rabbit Monoclonal Antibody (Pan-Actin兔单抗)(AG0115)
Pan-Actin Rabbit Monoclonal Antibody
一键复制产品信息 产品编号: AG0115
产品包装:50μl
选择包装
50μl
数
量
说明书下载
价格: ¥ 798.00
产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AG0115 | Pan-Actin Rabbit Monoclonal Antibody | 50μl | 798.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB, IHC-P, IF/ICC, FC | H, M, R | 42kDa | 42kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; ICC, Immunocytochemistry; IHC-P, Immunohistochemistry-Paraffin; mIHC-P, Multiplex IHC-P; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; Ar, Arabidopsis; C, Chicken; Cerastium, Ce; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Ph, Pharbitis; Pg, Pig; Rb, Rabbit; Rc, Rice; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF/ICC | IHC-P | mIHC-P | FC | ChIP | ChIP-seq |
| 1:500-1:1000 | – | 1:50 | 1:200-1:1000 | – | 1:50-1:100 | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | Pan-Actin Rabbit Monoclonal Antibody |
| Category | Rabbit Monoclonal Antibody (mAb); Primary Antibody; 未修饰抗体 |
| Isotype | IgG |
| Purification method | Protein A affinity purification |
| Positive samples | – |
| Cellular location | 细胞骨架 |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | – |
| Sequence | – |
| Gene ID | – |
| SwissProt | – |
| Synonyms | Alpha-actin-1, Alpha-actin-2, Alpha-actin-3, Alpha-cardiac actin, Beta-actin, Gamma-actin |
| Category | Actin Dynamics |
| Background | 肌动蛋白是高度保守的蛋白，参与各种类型的细胞运动，并在所有真核细胞中普遍表达。在脊椎动物中，已鉴定出三大类肌动蛋白亚型，即α，β和γ。α肌动蛋白存在于肌肉组织中，是收缩装置的主要组成部分。β和γ肌动蛋白在大多数细胞类型中作为细胞骨架的成分和内部细胞运动的介质共存。 |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AG0115 | Pan-Actin Rabbit Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
Pan-Actin Rabbit Monoclonal Antibody -20ºC保存，需避免反复冻融。Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖 蛋白膜。
c.回收稀释的一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。
4. 代表性图片。 
Fig. 1. Blocking buffer: QuickBlock™ Blocking Buffer (P0231); Detection: BeyoECL Star (P0018A) 一抗稀释度：1:1000 一抗孵育条件：室温2小时 二抗：HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution 裂 解 液：HeLa, Jurkat, HEK293, NIH/3T3, C2C12, mouse brain, rat brain 蛋白上样量：20 μg 曝光时间：60 s 理论分子量：42 kDa 实际分子量：42 kDa
Fig. 2. 组 织：人前列腺增生 切片类型：甲醛固定&石蜡包埋 修复方式：高温高压 修 复 液：Tris/EDTA (PH 9.0) 一抗稀释度：1:1000 一抗孵育条件：室温1小时 二抗：Anti-Rabbit and Mouse Polymer HRP (即用型) 胞核复染：苏木素(蓝色) 结果描述：棕色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号
Fig. 3. 组 织：人骨骼肌 切片类型：甲醛固定&石蜡包埋 修复方式：高温高压 修 复 液：Tris/EDTA (PH 9.0) 一抗稀释度：1:1000 一抗孵育条件：室温1小时 二抗：Anti-Rabbit and Mouse Polymer HRP (即用型) 胞核复染：苏木素(蓝色) 结果描述：棕色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号
Fig. 4. 组 织：小鼠心肌 切片类型：甲醛固定&石蜡包埋 修复方式：高温高压 修 复 液：Tris/EDTA (PH 9.0) 一抗稀释度：1:1000 一抗孵育条件：室温1小时 二抗：Anti-Rabbit and Mouse Polymer HRP (即用型) 胞核复染：苏木素(蓝色) 结果描述：棕色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号
Fig. 5. 组 织：大鼠胃 切片类型：甲醛固定&石蜡包埋 修复方式：高温高压 修 复 液：Tris/EDTA (PH 9.0) 一抗稀释度：1:1000 一抗孵育条件：室温1小时 二抗：Anti-Rabbit and Mouse Polymer HRP (即用型) 胞核复染：苏木素(蓝色) 结果描述：棕色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号
Fig. 6. 细 胞 系：HeLa 固定方式：100%冰甲醇 通透方式：0.1% Triton X-100 一抗稀释度：1:50 一抗孵育条件：4度过夜 二抗：Goat Anti-Rabbit IgG 胞核复染：DAPI(蓝色) 结果描述：绿色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号
Fig. 7. 细 胞 系：A431 固定方式：100%冰甲醇 通透方式：0.1% Triton X-100 一抗稀释度：1:50 一抗孵育条件：4度过夜 二抗：Goat Anti-Rabbit IgG 胞核复染：DAPI(蓝色) 结果描述：绿色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号
Fig. 8. 细 胞 系：NIH/3T3 固定方式：4%多聚甲醛 通透方式：0.1% Triton X-100 一抗稀释度：1:50 一抗孵育条件：4度过夜 二抗：Goat Anti-Rabbit IgG 胞核复染：DAPI(蓝色) 结果描述：绿色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号
Fig. 9. 细 胞 系：Jurkat 固定方式：4%多聚甲醛 通透方式：90% 甲醇 一抗稀释度：1: 100 二抗：Goat Anti-Rabbit IgG 阴性对照：未孵育一抗和二抗的细胞(黑色) 同型对照：兔单克隆IgG(蓝色) 结果描述：红色信号为Pan-Actin Rabbit Monoclonal Antibody的阳性信号
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖 蛋白膜。
c.回收稀释的一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2.免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页： http://www.beyotime.com/support/immunol-staining.htm
3.其它实验操作请自行参考适当的protocol进行。
4. 代表性图片。
Fig. 1. Blocking buffer: QuickBlock™ Blocking Buffer (P0231); Detection: BeyoECL Star (P0018A) 一抗稀释度：1:1000 一抗孵育条件：室温2小时 二抗：HRP-labeled Goat Anti-Rabbit IgG(H+L) (A0208) at 1:1000 dilution 裂 解 液：HeLa, Jurkat, HEK293, NIH/3T3, C2C12, mouse brain, rat brain 蛋白上样量：20 μg 曝光时间：60 s 理论分子量：42 kDa 实际分子量：42 kDa Fig. 2. 组 织：人前列腺增生 切片类型：甲醛固定&石蜡包埋 修复方式：高温高压 修 复 液：Tris/EDTA (PH 9.0) 一抗稀释度：1:1000 一抗孵育条件：室温1小时 二抗：Anti-Rabbit and Mouse Polymer HRP (即用型) 胞核复染：苏木素(蓝色) 结果描述：棕色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号 Fig. 3. 组 织：人骨骼肌 切片类型：甲醛固定&石蜡包埋 修复方式：高温高压 修 复 液：Tris/EDTA (PH 9.0) 一抗稀释度：1:1000 一抗孵育条件：室温1小时 二抗：Anti-Rabbit and Mouse Polymer HRP (即用型) 胞核复染：苏木素(蓝色) 结果描述：棕色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号 Fig. 4. 组 织：小鼠心肌 切片类型：甲醛固定&石蜡包埋 修复方式：高温高压 修 复 液：Tris/EDTA (PH 9.0) 一抗稀释度：1:1000 一抗孵育条件：室温1小时 二抗：Anti-Rabbit and Mouse Polymer HRP (即用型) 胞核复染：苏木素(蓝色) 结果描述：棕色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号 Fig. 5. 组 织：大鼠胃 切片类型：甲醛固定&石蜡包埋 修复方式：高温高压 修 复 液：Tris/EDTA (PH 9.0) 一抗稀释度：1:1000 一抗孵育条件：室温1小时 二抗：Anti-Rabbit and Mouse Polymer HRP (即用型) 胞核复染：苏木素(蓝色) 结果描述：棕色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号 Fig. 6. 细 胞 系：HeLa 固定方式：100%冰甲醇 通透方式：0.1% Triton X-100 一抗稀释度：1:50 一抗孵育条件：4度过夜 二抗：Goat Anti-Rabbit IgG 胞核复染：DAPI(蓝色) 结果描述：绿色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号 Fig. 7. 细 胞 系：A431 固定方式：100%冰甲醇 通透方式：0.1% Triton X-100 一抗稀释度：1:50 一抗孵育条件：4度过夜 二抗：Goat Anti-Rabbit IgG 胞核复染：DAPI(蓝色) 结果描述：绿色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号 Fig. 8. 细 胞 系：NIH/3T3 固定方式：4%多聚甲醛 通透方式：0.1% Triton X-100 一抗稀释度：1:50 一抗孵育条件：4度过夜 二抗：Goat Anti-Rabbit IgG 胞核复染：DAPI(蓝色) 结果描述：绿色染色为Pan-Actin Rabbit Monoclonal Antibody的阳性信号 Fig. 9. 细 胞 系：Jurkat 固定方式：4%多聚甲醛 通透方式：90% 甲醇 一抗稀释度：1: 100 二抗：Goat Anti-Rabbit IgG 阴性对照：未孵育一抗和二抗的细胞(黑色) 同型对照：兔单克隆IgG(蓝色) 结果描述：红色信号为Pan-Actin Rabbit Monoclonal Antibody的阳性信号 相关产品：
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Pan-Actin Rabbit mAb(Pan-Actin兔单抗)(AG8001)
Pan-Actin Rabbit Monoclonal Antibody
一键复制产品信息 产品编号: AG8001
产品包装:50μl
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50μl
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说明书下载
价格: ¥ 456.00
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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AG8001 | Pan-Actin Rabbit Monoclonal Antibody | 50μl | 456.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB, IHC, IF/ICC, IP | H, M, R, B, Dg, Mk, Pg, Z | 42kDa | 42kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; Ar, Arabidopsis; B, Bovine; C, Chicken; Cerastium, Ce; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Ph, Pharbitis; Pg, Pig; Rb, Rabbit; Rc, Rice; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:1,000-1:2,000 | 1:40 | 1:10-1:50 | 1:800-1:1,600 | 1:10-1:50 | – | – | – |
抗体详细信息如下:
| About this Antibody | |
| Name | Pan-Actin Rabbit Monoclonal Antibody |
| Category | Recombinant Rabbit Monoclonal Antibody (mAb); Primary antibody; 内参抗体 |
| Isotype | IgG |
| Purification method | Protein A affinity purification |
| Positive samples | – |
| Cellular location | Cytoplasm |
| Customer validation | – |
| About the Immunogen | |
| Immunogen | A synthetic peptide corresponding to the N-term of actin was used as an immunogen. |
| Sequence | – |
| Gene ID | 60, 58, 71, 70, 59, 72 |
| SwissProt | P60709, P68133, P63261, P68032, P62736, P63267 |
| Synonyms | all actin isoforms |
| Category | Cytoskeletal Signaling |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. This antibody is predicted to react with all actin isoforms, including: 1) sarcomeric actins, α-skeletal (α-SKA) and α-cardiac (α-CAA), 2) smooth muscle actins (SMAs), α-SMA and γ-SMA, and 3) cytoplasmic actins (CYAs), β-CYA and γ-CYA. |
包装清单：
| 产品编号 | 产品名称 | 包装 |
| AG8001 | Pan-Actin Rabbit Monoclonal Antibody | 50µl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件：
Pan-Actin Rabbit Monoclonal Antibody -20℃保存，需避免反复冻融。Western一抗稀释液-20℃或4℃保存，一年有效，Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000×g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片。 
All lanes: Anti-actin antibody at 1:1,000 dilution Predicted MW: 42 kDa Observed MW: 42 kDa Lane 1: Jurkat Lane 2: Hela Lane 3: 293T Lane 4: Raw264.7 Lane 5: A431 Lane 6: A375 Lane 7: 3T3 Lysates at 10 μg per lane 2nd Ab: GAR HRP(H+L) 1:5,000 Exposure: 20s
Actin was immunoprecipitated from 0.4mg of PC-12 whole cell lysate with Actin Rabbit Monoclonal Antibody at 1:40 dilution. 2nd Ab: GAR HRP for IP 1:500 Lane 1: Actin Rabbit Monoclonal Antibody IP in PC-12 whole cell lysate Lane 2: PBS instead of Actin Rabbit Monoclonal Antibody in PC-12 whole cell lysate Lane 3: PC-12 whole cell lysate, 10μg (input) Exposure: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic adenocarcinoma tissue labelling actin with Actin Rabbit Monoclonal Antibody at 1:1,600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Actin Rabbit Monoclonal Antibody staining Actin in HeLa cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:50) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1:500).
请根据抗体的实际用途选择相应的使用方法。
1.Western检测：
a.按照推荐的稀释比例用碧云天提供的Western一抗稀释液稀释抗体。
b.把经过封闭的蛋白膜与稀释好的一抗4℃缓慢摇动过夜或室温缓慢摇动2小时，确保稀释的抗体至少能在摇动的瞬间覆盖蛋白膜。
c.回收稀释的一抗，4℃保存以备下次继续使用。
d.按照Western的实验步骤进行后续的洗涤、二抗孵育、洗涤和检测等操作。具体操作可以参考如下网页：
http://www.beyotime.com/support/western.htm
2. 免疫染色：
可以使用碧云天生产的免疫染色一抗稀释液(P0103)稀释抗体，使用后注意回收稀释好的一抗，具体操作可以参考如下网页：http://www.beyotime.com/support/immunol-staining.htm
3. 其它实验操作请自行参考适当的protocol进行。
4. 代表性图片。
All lanes: Anti-actin antibody at 1:1,000 dilution Predicted MW: 42 kDa Observed MW: 42 kDa Lane 1: Jurkat Lane 2: Hela Lane 3: 293T Lane 4: Raw264.7 Lane 5: A431 Lane 6: A375 Lane 7: 3T3 Lysates at 10 μg per lane 2nd Ab: GAR HRP(H+L) 1:5,000 Exposure: 20s Actin was immunoprecipitated from 0.4mg of PC-12 whole cell lysate with Actin Rabbit Monoclonal Antibody at 1:40 dilution. 2nd Ab: GAR HRP for IP 1:500 Lane 1: Actin Rabbit Monoclonal Antibody IP in PC-12 whole cell lysate Lane 2: PBS instead of Actin Rabbit Monoclonal Antibody in PC-12 whole cell lysate Lane 3: PC-12 whole cell lysate, 10μg (input) Exposure: 30s Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colonic adenocarcinoma tissue labelling actin with Actin Rabbit Monoclonal Antibody at 1:1,600. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0. Actin Rabbit Monoclonal Antibody staining Actin in HeLa cells by IF/ICC. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:50) at 4°C. An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (1:500). 相关产品：
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| P0098 | 免疫染色固定液 | 100ml/500ml |
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