辣根过氧化物酶标记驴抗山羊IgG(H+L)
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辣根过氧化物酶标记驴抗山羊IgG(H+L)(A0181)
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价格: ¥ 213.00
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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| A0181 | 辣根过氧化物酶标记驴抗山羊IgG(H+L) | 0.2ml | 213.00元 |
| 来源 | 用途 | WB | ELISA | IHC |
| Donkey | WB, ELISA, IHC | 1:1000-2000 | 1:250-500 | 1:50-100 |
WB, Western blot; IHC, Immunohistochemistry.
本辣根过氧化物酶标记驴抗山羊IgG(H+L)(HRP-labeled Donkey Anti-Goat IgG(H+L))为进口分装，用于Western、ELISA和IHC等的二抗。HRP，即horseradish peroxidase，中文名为辣根过氧化物酶，可以在Western检测时催化ECL类试剂(如ECL、BeyoECL等)产生化学发光，可以在ELISA时催化TMB产生蓝色，也可以在IHC或Western blot检测时催化DAB产生棕色沉淀。
本辣根过氧化物酶标记驴抗山羊IgG(H+L)用于WB、ELISA和IHC的推荐稀释比例参考上表，实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。例如对于WB，推荐的可以调节的稀释范围为1:500-5000。
本抗体为用纯化的山羊IgG免疫驴，然后用亲和纯化柱对获得的抗血清进行纯化，并经过人、兔、小鼠、大鼠、豚鼠、仓鼠、马、鸡血清吸附纯化的优质二抗。对人、兔、小鼠、大鼠、豚鼠、仓鼠、马、鸡血清蛋白几乎没有结合能力(Minimal Cross-reaction to Human, Rabbit, Mice, Rat, Guinea Pig, Syrian Hamster, Horse and Chicken Serum Proteins)。
本抗体如果用于常规的Western检测，以每次检测需10毫升1:1000稀释的二抗计，可以检测20次。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| A0181 | 辣根过氧化物酶标记驴抗山羊IgG (H+L) | 0.2ml |
| — | 说明书 | 1份 |
保存条件：
-20℃保存，一年有效。4℃保存，半年有效。
注意事项：
叠氮钠会抑制辣根过氧化物酶的活性，在含有辣根过氧化物酶的二抗中不可加入叠氮钠。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1.Western、ELISA或IHC请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
1.Western、ELISA或IHC请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
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HRP标记二抗
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碱性磷酸酯酶标记驴抗山羊IgG(H+L)(A0266)
碱性磷酸酯酶标记驴抗山羊IgG(H+L)
一键复制产品信息 产品编号: A0266
产品包装:0.5ml
选择包装
0.5ml
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说明书下载
价格: ¥ 469.00
产品简介
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相关产品
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产品问答
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| A0266 | 碱性磷酸酯酶标记驴抗山羊IgG(H+L) | 0.5ml | 469.00元 |
| 来源 | 用途 | WB | ELISA | IHC |
| Donkey | WB, ELISA, IHC | 1:500-5000 | 1:500-5000 | 1:500-2000 |
WB, Western blot; IHC, Immunohistochemistry.
本碱性磷酸酯酶标记驴抗山羊IgG(H+L)(AP-labeled Donkey Anti-Goat IgG(H+L))为进口分装，用于Western、ELISA和IHC等的二抗。
碱性磷酸酯酶(Alkaline Phosphatase, AP/ALP/AKP/ALKP/ALPase/Alk Phos)常被称作碱性磷酸酶(EC 3.1.3.1)，是一类水解酶，通过水解磷酸单酯将底物分子上的磷酸基团除去，并生成磷酸根离子和自由的羟基，其去磷酸化作用的底物包括蛋白质、核苷酸和生物碱等，并在碱性条件下最为有效。该酶是一组同功酶的统称。常见的小牛肠碱性磷酸酶(Calf Intestinal Alkaline Phosphatase, CIAP/CIP)被广泛用于二抗等的标记最终用于蛋白和核酸等的检测，例如在Western、ELISA、IHC、ICC、Northern、Southern等时，催化BCIP/NBT等显色试剂显色或催化适当的化学发光试剂产生化学发光。本产品标记的就是小牛肠碱性磷酸酶。
本碱性磷酸酯酶标记驴抗山羊IgG(H+L)用于WB、ELISA和IHC的推荐稀释比例参考上表，实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。
本抗体为用纯化的山羊IgG免疫驴，然后用亲和纯化柱对获得的抗血清进行纯化，并经过人、兔、小鼠、大鼠、豚鼠、仓鼠、马、鸡血清吸附纯化的优质二抗。对人、兔、小鼠、大鼠、豚鼠、仓鼠、马、鸡血清蛋白几乎没有结合能力(Minimal Cross-reaction to Human, Rabbit, Mice, Rat, Guinea Pig, Syrian Hamster, Horse and Chicken Serum Proteins)。
本抗体如果用于常规的Western检测，以每次检测需10毫升1:1000稀释的二抗计，可以检测50次。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| A0266 | 碱性磷酸酯酶标记驴抗山羊IgG (H+L) | 0.5ml |
| — | 说明书 | 1份 |
保存条件：
-20℃保存，一年有效。4℃保存，2个月有效。
注意事项：
BSA或脱脂奶粉可以和anti-Goat IgG产生轻微的交叉反应，因此BSA或脱脂奶粉作为封闭试剂或用于抗体的稀释时，会导致背景升高或二抗的效价有所下降。使用anti-Goat IgG时，总体上还是可以用BSA或脱脂奶粉作为封闭试剂或抗体的稀释试剂，但如果使用其它合适的试剂则效果更佳。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. Western、ELISA或IHC请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
质检证书
输入批号搜寻COA
搜索
相关产品请点击如下按钮：
ALP标记二抗
使用本产品的文献：
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