Avidity BirA生物素蛋白连接酶标准反应试剂盒说明书

Avidity,LLC于1996年由Millard Cull,Larry Lansing,Ron Gill博士和Mark Seville博士创立,基于生物素对抗生物素蛋白或链霉亲和素的非凡亲和力开发肽标签和分子生物学产品。在Affymax担任研究人员的同时,Millard参与了AviTag技术的发现,发现了其商业潜力,并获得了Affymax的*权利。他开始使用Avidity作为试剂公司,将AviTag技术的使用权授予其他公司,并销售与该技术一起使用的产品。

带有AviTag标签的人类全长ORF克隆已经被构建在多种T7和CMV启动子表达系统,可以立即购买使用。AviTag标签蛋白可以被体内或体外的生物素连接酶生物素化,而且抗生物素蛋白或链霉亲和素可以专一地与生物素结合,正基于这两个反应,AviTag?技术可以应用于蛋白的固定,纯化和显影。 虽然Avidity是一个小公司,科学带来的好处及其AviTag已得到认可。目前, AviTag技术已经在22个国家的世界制药公司和研究人员中得到认可。 

随着Avidity成熟,该公司正在扩大其科学视野,并调查使用生物发光 – 如在深海生物体中发现 – 使其产品对科学界更有用。Prolume有限公司的Bruce Bryan博士于2004年接触AvidTag与Prolume的高斯荧光素酶和绿色荧光蛋白(GFP)结合使用。*款高斯AviTag产品于2007年11月发布。

此外,Avidity正在与几家公司合作,通过将非常灵敏的光学传感器与基于荧光素酶的测定相结合,开发出新一代高度移动,简单,廉价且功能强大的诊断功能。结合BP蛋白质组学(新型测定生物化学),Prolume(发光/荧光分子和硬件创新专家),黑森林工程(光学器件工程),Avidity正在开发用于检测和鉴定的蛋白质和DNA微阵列生物芯片和生物传感器的疾病病原体和复合高通量筛选(HTS)。

 

 

  BirA生物素蛋白连接酶标准反应试剂盒

货号:BirA500 

品名:BirA-500: BirA biotin-protein ligase standard reaction kit

 

产品信息

生物素蛋白连接酶(BirA的EC 6.3.4.15)activates形成生物素生物素和5′- adenylate转移到生物素蛋白的生物素接受(如avitag™肽)。它也抑制功能的生物素操纵子。该基因编码的蛋白是由BirA的基因。

 

BirA生物素 – 蛋白质连接酶(EC 6.3.4.15)以和靶向的方式通过ATP中间体(生物素5'-腺苷酸)共价加入生物素受体肽/蛋白质中的d生物素。酶促生物素化蛋白质的下游应用是多样的,重要和有力的。*的生物素 – 抗生物素蛋白/链霉抗生物素蛋白相互作用通常用于亲和层析或蛋白质固定在表面或底物上。通过抗生物素抗体或抗生物素蛋白/链霉亲和素 – 报道酶结合物(-HRP,碱性磷酸酶)或荧光探针进行蛋白质检测成为可能。生物素化MHC分子的多聚体形式是免疫生物学中的常用工具。

当与我们的AviTag TM生物素受体肽氨基酸序列组合使用时,生物素化发生在天然大肠杆菌BCCP底物的两倍,比其他肽序列的生物素化多达一个数量级。因此,与其他可用序列相比,AviTagTM序列需要较少的BirA酶和较短的生物素化孵育时间才能完成。如果蛋白质不稳定或蛋白酶活动是关注的,这对下游成功可能是重要的。

我们的BirA酶是由birA基因编码的大肠杆菌野生型,并通过传统方法纯化至大于99%的纯度。

该酶的其他名称包括:生物素连接酶;生物素操纵子阻遏蛋白;的birA;生物素全酶合成酶;生物素 – [乙酰-CoA羧化酶]合成酶;生物素 -[乙酰辅酶A羧化酶]连接酶;生物素 -[乙酰-CoA羧化酶]合成酶;乙酰CoA高脱羧酶合成酶;乙酰CoA高脱羧酶合成酶;生物素:apocar​boxylase连接酶;生物素全酶合成酶;HCS。

 

试剂盒组成:

两瓶,每瓶20uL1mg/mL BirA生物素蛋白连接酶(共40ug)

两瓶,每瓶1.5mLBiomixA(0.5M双甘酯缓冲溶液,pH8.3)

两瓶,每瓶1.5mLBiomixB(100mM ATP,100mM镁(OAC)2,500uM d-生物素)

两瓶,每瓶1.5mL额外的BIO-200(500uM d-生物素)

 

储存条件:

试剂盒,干冰运输,-80度保存。如解冻后使用,4度保存。解冻后的酶可以在液氮中重新储存,-80度储存。

其他组份,biomixa,biomixb和bio-200,-20度长期保存。biomixa和bio-200,在4度可短期储存。Biomixb,-20度保存或者更低温度储存。

稳定性:生物素蛋白连接酶BirA,4度存放一个月,活性保留>80。长期保存-80度,每次解冻,酶活性降低10%。

实验步骤:

以700 µl纯化好的 MHC/peptide 复合物(1-2mg)为例,加入:100 µl BiomixA,100 µl BiomixB,100 µl d-biotin,10 µl BirA enzyme (30 µg),0.5 ul pepstatin (2 mg/ml in DMSO),0.5 µl leupeptin (2 mg/ml in dH2O)室温孵育过夜(反应液中不要有EDTA)

 

 

 

https://www.avidity.com/avitag/bira-500-bira-biotin-protein-ligase-standard-reaction-kit

 

citeqbiologics抗体和生物素化试剂盒

山羊抗小鼠抗体

结合我们的生物素化 HDM 过敏原产品,抗体可用于使用 ELISA 方法量化小鼠血清中的 HDM 过敏原特异性 IgE/IgG1/IgG2a 抗体。该抗体可以包被在 ELISA 板上并作为捕获抗体来固定血清 IgE/IgG1/IgG2a 抗体。在下一步中,添加生物素化的 HDM 过敏原以促进检测 IgE/IgG1/IgG2a 同种型的 HDM 过敏原特异性抗体。使用参考血清或通过比较多种血清,可以(相对)量化 HDM 过敏原特异性抗体。

对于正在使用小鼠模型进行过敏性哮喘研究的研究人员来说,可以通过查看小鼠血清中针对 HDM 蛋白的特异性抗体的数量来确定对屋尘螨 (HDM) 小鼠的免疫是否成功。

citeqbiologics抗体和生物素化试剂盒

描述

抗体和生物素化试剂盒

我们的多克隆抗小鼠抗体可与我们的生物素化 HDM 过敏原产品一起使用,以使用 ELISA 方法量化小鼠血清中的 HDM 过敏原特异性抗体。该试剂盒包含 IgE/ IgG1/ IgG2a 抗体和生物素化 D. pteronyssinus HDM。

抗原特异性血清抗体的定量

该抗体可以包被在 ELISA 板上并作为捕获抗体来固定血清 IgE/IgG1/IgG2a 抗体。在下一步中,添加生物素化的 HDM 过敏原以促进检测 IgE/IgG1/IgG2a 同种型的 HDM 过敏原特异性抗体。使用参考血清或通过比较多种血清,可以(相对)量化 HDM 过敏原特异性抗体。

对于正在使用小鼠模型进行过敏性哮喘研究的研究人员来说,可以通过查看小鼠血清中针对 HDM 蛋白的特异性抗体的数量来确定对屋尘螨 (HDM) 小鼠的免疫是否成功。

捕获的 HDM 抗体的特异性必须使用生物素化 HDM 或生物素化主要过敏原(如生物素化 Der p 1 或生物素化 Der p 2)进行测试。 抗体和生物素化产品可作为单独的产品提供,但也可在包含抗小鼠 IgE/抗小鼠 IgG1/抗小鼠 IgG2A 和生物素化的 HDM 提取物(或生物素化的 Der p 1/生物素化的 Der p 2)。

套件内容

  • 1 x 抗小鼠 IgE(足以包被 1 x 96 孔板)

  • 1 x 抗小鼠 IgG1(足以包被 1 x 96 孔板)

  • 1 x 抗小鼠 IgG2A(足以包被 1 x 96 孔板)

  • 您可以从以下生物素化产品中进行选择:

    • 生物素化 D. pteronyssinus (HDM) 提取物

    • 生物素化 Der p 1

    • 生物素化的 Der p 2

 

重组生物素化人LAG3/CD223蛋白(His-Avi标记) Human LAG3/CD223 Protein

重组生物素化人LAG3/CD223蛋白(His-Avi标记) Human LAG3/CD223 Protein

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

LAG3; FDC;CD223; sLAG-3

表达区间及表达系统(Source)

Biotinylated Human LAG3/CD223 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu23-Gly434.[Accession | P18627-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Anti-LAG3 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12.7ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

LAG3; FDC;CD223; sLAG-3

表达区间及表达系统(Source)

Biotinylated Human LAG3/CD223 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu23-Gly434.[Accession | P18627-1]

分子量大小(Molecular Weight)

The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

ELISA Data: Immobilized Anti-LAG3 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12.7ng/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

重组生物素化人CEACAM-8/CD66b蛋白 Recombinant Biotinylated Human CEACAM-8/CD66b Protein,His-Avi Tag

重组生物素化人CEACAM-8/CD66b蛋白 Recombinant Biotinylated Human CEACAM-8/CD66b Protein,His-Avi Tag

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

CD66b; CEACAM8; CGM6; CD67; NCA-95

表达区间及表达系统(Source)

Biotinylated Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln35-Ser319.[Accession | NP_001807.2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

SPR Data:Biotinylated Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 6.13 μM as determined in SPR assay .

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

CD66b; CEACAM8; CGM6; CD67; NCA-95

表达区间及表达系统(Source)

Biotinylated Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gln35-Ser319.[Accession | NP_001807.2]

分子量大小(Molecular Weight)

The protein has a predicted MW of 34.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE and HPLC.

活性(Activity)

SPR Data:Biotinylated Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 6.13 μM as determined in SPR assay .

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

1.The product should be stored at -25~-15℃ for 1 year from date of receipt.

2.2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3.3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

4.Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

metkinenchemistry 生物素-11-dCTP产品简介

metkinenchemistry 生物素-11-dCTP产品简介

生物素-11-dCTP

5-[3-(6-(生物素酰氨基)己酰氨基)丙烯基]-2'-脱氧胞苷-5'-三磷酸、三乙铵或四锂(可选)盐



目录号: metkinenchemistry 303-21

纯度: > 95%

稀释剂: dd-H 2 O

储存:冷冻储存,-10 至 -30°C,干燥。

溶液稳定性: 1 mM 水溶液或中性缓冲液在 +4°C 下可保存 1 个月,1 mM 水溶液或中性缓冲液在 -20°C 下可保存 6 个月。

Metkinen Chemistry专注于 RNA 和 DNA 合成试剂的研究和制造。我们拥有核苷化学、寡核苷酸合成试剂、亚磷酰胺和各种固体支持物方面的专业知识。寡核苷酸合成产品线的试剂包括 Truly Universal 固体支持物、化学磷酸化试剂和三聚体(密码子)亚磷酰胺。可能的应用包括具有三聚体(密码子)亚磷酰胺的组合寡核苷酸文库、使用我们的 Truly Universal 固相支持物进行寡核苷酸合成以及使用化学磷酸化试剂方便地纯化寡核苷酸 5'-磷酸。今天,Metkinen Chemistry 的努力集中在我们广泛的嘌呤核苷系列上. 超纯嘌呤核苷类似物用于寡核苷酸疗法的开发和研究应用。新产品,即3'-Amino-2',3'-dideoxy-AG核苷可作为抗酶降解寡核苷酸的构建模块。另一组新核苷 – 2'-deoxy-2'-fluoro-AG,以及2'-deoxy-2'-amino-AG是用于稳定小干扰 RNA (siRNA) 和适体的流行构建时钟。Metkinen Chemistry 的生物转化技术解决了传统合成方法的问题,并提供了生物转化的替代变体。我们的灵活性和专业知识使 Metkinen Chemistry 能够满足一系列客户需求:从学术界的一次性寡核苷酸合成到大规模商业生产。

SV0050 超级链霉抗生物素蛋白链珠

Ocean Nanotech总部位于加州圣地亚哥,是一家私人拥有的生物技术公司。我们是用于研发和诊断应用的各种纳米粒子的制造商。结合我们强大的分子生物学和细胞生物学专长与纳米粒子技术和平台,我们开发和销售各种基于磁性粒子的生物制药和体外诊断工具。我们继续与学术界,生物技术和制药行业的合作伙伴密切合作,开发新产品和应用。我们茁壮成长为我们的合作伙伴提供基于纳米/微米颗粒的综合解决方案。

Ocean Nanotech  SV0050  超级链霉抗生物素蛋白链珠

 

这些是直径为50nm的链霉抗生物素蛋白缀合的Super Mag珠粒。在与表面共价偶联的单层重组链霉抗生物素蛋白之前,将磁珠用一层BSA预先封闭。链霉亲和素的单层使得大部分生物素结合位点在空间上可用于结合生物素化的核酸,抗体或其他生物素化的配体和靶标。50nm超级链霉亲和素珠具有高容量,的磁拉力,并且在孵育期间不会沉降。

特征:

  • 高结合能力
  • 与蛋白质和核酸的非特异性结合显着较低
  • 由于磁含量比例高,可以通过永磁体和高梯度磁柱进行分离

应用:

理想的细胞分离和检测开发。蛋白质和核酸的非特异性结合很低。

Binding capacity:

一毫克的50纳米超级磁珠通常绑定:

  • > 5,500 pmoles游离生物素
  • 〜850pmol生物素化肽
  • >35μg生物素化的IgG
  • 〜35μgds-DNA
  • 〜1,100pmol的ss-寡核苷酸

规范:

产品描述:链亲和素缀合的超顺磁珠
溶剂:  去离子水,0.05%的NaN 3,0.01%吐温20,0.1%BSA 
浓度: 1mg / mL的
通过动态光散射粒度: 50-80纳米
的表面配体:链亲和素
磁性含量: > 80%
1 mL中的珠粒数量: 6×10 11 
储存: 2-8°C; 不要冻结。保持小瓶直立。

目录#

产品

单位大小

SV0051

超级链霉抗生物素蛋白链珠-1毫升

1毫升

SV0052

超级链霉抗生物素蛋白链珠-5毫升

5毫升

SV0053

超级链霉抗生物素蛋白链珠 – 10毫升

10毫升

 

浓度

1毫克/毫升

粒度

0.05

产品描述

超级链霉亲和素珠,0.05μm

表面

亲水

功能

链亲和素

这些是直径为50nm的链霉抗生物素蛋白缀合的Super Mag珠粒。在与表面共价偶联的单层重组链霉抗生物素蛋白之前,将磁珠用一层BSA预先封闭。链霉亲和素的单层使得大部分生物素结合位点在空间上可用于结合生物素化的核酸,抗体或其他生物素化的配体和靶标。50nm超级链霉亲和素珠具有高容量,的磁拉力,并且在孵育期间不会沉降。

特征:

  • 高结合能力
  • 与蛋白质和核酸的非特异性结合显着较低
  • 由于磁含量比例高,可以通过永磁体和高梯度磁柱进行分离

应用:

理想的细胞分离和检测开发。蛋白质和核酸的非特异性结合很低。

装订能力:

一毫克的50纳米超级磁珠通常绑定:

  • > 5,500 pmoles游离生物素
  • 〜850pmol生物素化肽
  • >35μg生物素化的IgG
  • 〜35μgds-DNA
  • 〜1,100pmol的ss-寡核苷酸

规范:

产品描述:链亲和素缀合的超顺磁珠
溶剂:  去离子水,0.05%的NaN 3,0.01%吐温20,0.1%BSA 
浓度: 1mg / mL的
通过动态光散射粒度: 50-80纳米
的表面配体:链亲和素
磁性含量: > 80%
1 mL中的珠粒数量: 6×10 11 
储存: 2-8°C; 不要冻结。保持小瓶直立。

1.1产品标识符

产品名称:Super Mag Streptavidin Beads  

目录号:SV0050 – SV0200

 1.2物质或混合物的相关确定用途和不适宜的用途

已确定的用途:实验室化学品,物质的制造

 

 

Streptavidin 系列产品

Product Name

Description

 

 

SV0050

Super Mag Streptavidin Beads, 0.05 µm

 

 

Catalog#
SV0051

Particle Size(µm)
0.05

Concentration
1 mg/mL

Amount
1 mL

Catalog#
SV0052

Particle Size(µm)
0.05

Concentration
1 mg/mL

Amount
5 mL

Catalog#
SV0053

Particle Size(µm)
0.05

Concentration
1 mg/mL

Amount
10 mL

SV0100

Super Mag Streptavidin Beads, 0.1 µm

 

 

Catalog#
SV0101

Particle Size(µm)
0.1

Concentration
1 mg/mL

Amount
1 mL

Catalog#
SV0102

Particle Size(µm)
0.1

Concentration
1 mg/mL

Amount
5 mL

Catalog#
SV0103

Particle Size(µm)
0.1

Concentration
1 mg/mL

Amount
10 mL

HV0150

Hi-Sur Mag Streptavidin Beads, 0.15 µm

 

 

Catalog#
HV0151

Particle Size(µm)
0.15

Concentration
1 mg/mL

Amount
1 mL

Catalog#
HV0152

Particle Size(µm)
0.15

Concentration
1 mg/mL

Amount
5 mL

Catalog#
HV0153

Particle Size(µm)
0.15

Concentration
1 mg/mL

Amount
10 mL

SV0150

Super Mag Streptavidin Beads, 0.15 µm

 

 

Catalog#
SV0151

Particle Size(µm)
0.15

Concentration
1 mg/mL

Amount
1 mL

Catalog#
SV0152

Particle Size(µm)
0.15

Concentration
1 mg/mL

Amount
5 mL

Catalog#
SV0153

Particle Size(µm)
0.15

Concentration
1 mg/mL

Amount
10 mL

SV0200

Super Mag Streptavidin Beads, 0.2 µm

 

 

Catalog#
SV0201

Particle Size(µm)
0.2

Concentration
1 mg/mL

Amount
1 mL

Catalog#
SV0202

Particle Size(µm)
0.2

Concentration
1 mg/mL

Amount
5 mL

Catalog#
SV0203

Particle Size(µm)
0.2

Concentration
1 mg/mL

Amount
10 mL

MV0500

Mono Mag Streptavidin Beads, 0.5 µm

 

 

Catalog#
MV0501

Particle Size(µm)
0.5

Concentration
10 mg/mL

Amount
2 mL

Catalog#
MV0502

Particle Size(µm)
0.5

Concentration
10 mg/mL

Amount
10 mL

MV1000

Mono Mag Streptavidin Beads, 1 µm

 

 

Catalog#
MV1001

Particle Size(µm)
1

Concentration
10 mg/mL

Amount
2 mL

Catalog#
MV1002

Particle Size(µm)
1

Concentration
10 mg/mL

Amount
10 mL

HV1000

Hi-Sur Mag Streptavidin Beads, 1 µm

 

 

Catalog#
HV1001

Particle Size(µm)
1

Concentration
10 mg/mL

Amount
2 mL

Catalog#
HV1002

Particle Size(µm)
1

Concentration
10 mg/mL

Amount
10 mL

Catalog#
HV1003

Particle Size(µm)
1

Concentration
10 mg/mL

Amount
100 mL

MV3000

Mono Mag Streptavidin Beads, 3 µm

 

 

Catalog#
MV3001

Particle Size(µm)
3

Concentration
10 mg/mL

Amount
2 mL

Catalog#
MV3002

Particle Size(µm)
3

Concentration
10 mg/mL

Amount
10 mL

MV4500

Mono Mag Streptavidin Beads, 4.5 µm

 

 

Catalog#
MV4501

Particle Size(µm)
4.5

Concentration
10 mg/mL

Amount
2 mL

Catalog#
MV4502

Particle Size(µm)
4.5

Concentration
10 mg/mL

Amount
10 mL

 

上海金畔生物科技有限公司是实验试剂一站式采购服务商

1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

2:产品种类齐全,经营超过700多个品牌,基本涵盖所有生物实验试剂耗材。

3:提供加急服务,货品一般1-2周到货。

4:富有竞争力的价格优势,绝大部分价格有优势。

5:多年积累良好的信誉,大部分客户提供货到付款服务。客户包括清华、北大、交大、复旦、中山等100多所高校,ROCHE,阿斯利康、国药、fisher等药企。

6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。

 

 

重组生物素化小鼠PVRIG蛋白(hFc-Avi标签) Mouse PVRIG Protein

重组生物素化小鼠PVRIG蛋白(hFc-Avi标签) Mouse PVRIG Protein

产品说明书

FAQ

COA

已发表文献

 

性能参数

分子别名(Synonyms)

MGC104322; CD112R;C7orf15; MGC2463

表达区间及表达系统(Source)

Biotinylated Mouse PVRIG Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Ser35-Asp165.[Accession | A0A1B0GS01]

分子量大小(Molecular Weight)

The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE

活性(Activity)

ELISA Data: Immobilized Mouse Nectin-2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 1.76μg/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

 

性能参数

分子别名(Synonyms)

MGC104322; CD112R;C7orf15; MGC2463

表达区间及表达系统(Source)

Biotinylated Mouse PVRIG Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Ser35-Asp165.[Accession | A0A1B0GS01]

分子量大小(Molecular Weight)

The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result.

内毒素(Endotoxin)

Less than 1EU per μg by the LAL method.

纯度(Purity)

> 95% as determined by SDS-PAGE

活性(Activity)

ELISA Data: Immobilized Mouse Nectin-2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 1.76μg/ml determined by ELISA.

制剂(Formulation)

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

重构方法(Reconstitution)

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

 

储存条件

The product should be stored at -25~-15℃ for 1 year from date of receipt.

2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.

3 -6 months, -85~-65℃ under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

注意事项

1.Please operate with lab coats and disposable gloves,for your safety.

2.This product is for research use only.

生物素高效标记山羊抗兔IgG(H+L)(A0279)

生物素高效标记山羊抗兔IgG(H+L)
生物素高效标记山羊抗兔IgG(H+L)(A0279) 一键复制产品信息

产品编号: A0279

产品包装:0.5ml
选择包装

0.5ml

说明书下载

262.00 253.00 10

价格: ¥ 262.00

促销价: ¥ 262.00

促销价: ¥ 262.00 253.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
A0279 生物素高效标记山羊抗兔IgG(H+L) 0.5ml 262.00元

来源 用途 WB ICC/IF IHC ELISA
Goat WB, ICC, IF, IHC, ELISA 1:500-5000 1:50-200 1:50-200 1:2000-30000

WB, Western blot; ICC, Immunocytochemistry; IF, Immunofluorescence; IHC, Immunohistochemistry; ELISA, Enzyme-linked immune-sorbent assay.
本生物素高效标记山羊抗兔IgG(H+L) (Biotin-labeled Goat Anti-Rabbit IgG(H+L) with high mole ratio)为进口分装,用于Western、免疫细胞化学、免疫荧光、免疫组化和ELISA等检测的高纯度高特异性二抗。
生物素标记后可以采用SABC(Streptavidin-Biotin Complex)法进行检测;也可以使用辣根过氧化物酶标记的Streptavidin或碱性磷酸酯酶标记的Streptavidin来识别生物素,后续用辣根过氧化物酶或碱性磷酸酯酶对应的检测体系进行显色反应或发光反应等。
本抗体为用纯化的兔IgG(H+L)免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过小鼠IgG等吸附纯化和生物标记的优质二抗。对小鼠等的血清蛋白几乎没有结合能力(Minimal Cross-reaction to Mouse)。
本生物素高效标记山羊抗兔IgG(H+L)每摩尔IgG结合多摩尔的生物素,能结合多个streptavidin或avidin分子,在SABC或ABC等检测系统中,能有效放大信号,提高检测灵敏度。
本生物素高效标记山羊抗兔IgG(H+L)用于Western、免疫细胞化学、免疫荧光、免疫组化和ELISA的推荐稀释比例参考上表,实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。
本抗体如果用于常规的Western检测,以每次检测需10毫升1:2000稀释的二抗计算,可以检测100次;如果用于常规的免疫细胞化学或免疫组化检测,按照每样需要100微升1:100稀释的本抗体计算,可以检测500个样品。
包装清单:

产品编号 产品名称 包装
A0279 生物素高效标记山羊抗兔IgG(H+L) 0.5ml
说明书 1份

保存条件:
-20℃保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1.Western、免疫细胞化学、免疫荧光、免疫组化和ELISA请参考相关实验步骤进行。起始稀释浓度请按照产品简介中推荐的稀释比例进行稀释。

相关产品:

产品编号 产品名称 包装
A0277 生物素标记山羊抗兔IgG(H+L) 1ml
A0279 生物素高效标记山羊抗兔IgG(H+L) 0.5ml
A0286 生物素标记山羊抗小鼠IgG(H+L) 1ml
A0288 生物素高效标记山羊抗小鼠IgG(H+L) 0.5ml
A0303 辣根过氧化物酶标记Streptavidin 0.2ml
A0312 碱性磷酸酯酶标记Streptavidin 0.2ml
P0100A 内源性过氧化物酶封闭液 100ml
P0100B 内源性过氧化物酶强力封闭液 100ml
P0100C 内源性碱性磷酸酶封闭液 6ml
P0101 生物素检测封闭试剂盒 200次
P0603 SABC-HRP Kit (IHC, ICC, Blotting & ELISA) 1000次
P0606 SABC-AP Kit (IHC, ICC, Blotting & ELISA) 1000次
P0612 SABC-HRP Kit with Anti-Mouse IgG (IHC & ICC) 500次
P0615 SABC-HRP Kit with Anti-Rabbit IgG (IHC & ICC) 500次
P0625 SABC-AP Kit with Anti-Mouse IgG (IHC & ICC) 500次
P0628 SABC-AP Kit with Anti-Rabbit IgG (IHC & ICC) 500次

生物素高效标记山羊抗兔IgG(H+L)(A0279)
生物素高效标记山羊抗兔IgG(H+L)(A0279)

物质安全数据单
输入右侧验证码点击下载:

生物素高效标记山羊抗兔IgG(H+L)(A0279)

生物素高效标记山羊抗兔IgG(H+L)(A0279)
质检证书
输入批号搜寻COA

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相关产品请点击如下按钮:
Biotin标记二抗
免疫染色

使用本产品的文献:

1. Ni-Ya Wang, Jin-Nan Li, Wei-Lin Liu, Qi Huang, Wen-Xing Li, Ya-Hong Tan, Fang Liu, Zi-Hua Song, Meng-Yue Wang, Ning Xie, Rong-Rong Mao, Ping Gan, Yu-Qiang Ding, Zhi Zhang, Bao-Ci Shan, Li-Dian Chen, Qi-Xin Zhou, Lin Xu
Ferulic Acid Ameliorates Alzheimer’s Disease-like Pathology and Repairs Cognitive Decline by Preventing Capillary Hypofunction in APP/PS1 Mice
Neurotherapeutics. 2021 Apr;18(2):1064-1080.;doi: 10.1007/s13311-021-01024-7. (IF 6.035)

2. Jiayi Lin, Jiacheng Du, Xiexing Wu, Congxin Xu, Jiangtao Liu, Luyong Jiang, Xiaoqiang Cheng, Gaoran Ge, Liang Chen, Qingjiang Pang, Dechun Geng, Haiqing Mao
SIRT3 mitigates intervertebral disc degeneration by delaying oxidative stress-induced senescence of nucleus pulposus cells
J Cell Physiol. 2021 Sep;236(9):6441-6456.;doi: 10.1002/jcp.30319. (IF 5.546)

3. Gan P, Xia Q, Hang G, Zhou Y, Qian X, Wang X, Ding L
Knockdown of cathepsin D protects dopaminergic neurons against neuroinflammation-mediated neurotoxicity through inhibition of NF-κB signalling pathway in Parkinson’s disease model.
CLIN EXP PHARMACOL P . 2018 Nov 28. (IF 2.092)

4. Hui GD, Xiu WY, Yong C, Yuan CB, Jun Z, Jun GJ, Jun YJ, Xiang XX, Wei HS, Feng ML
AMP-activated protein kinase α1 serves a carcinogenic role via regulation of vascular endothelial growth factor expression in patients with non-small cell lung cancer.
Oncol Lett. 2019 May;17(5):4329-4334. (IF 2.311)

5. Ding Y, He J, Huang J, Yu T, Shi X, Zhang T, Yan G, Chen S, Peng C
Harmine induces anticancer activity in breast cancer cells via targeting TAZ.
Int J Oncol. 2019 Jun;54(6):1995-2004. (IF 3.899)

6. Han L, Zan Y, Huang C, Zhang S
NELFE promoted pancreatic cancer metastasis and the epithelial‑to‑mesenchymal transition by decreasing the stabilization of NDRG2 mRNA.
Int J Oncol. 2019 Dec;55(6):1313-1323. (IF 3.899)

7. Guangfeng Zhang, Huafeng Li, Wenjie Zhao, Min Li, Linlin Tian, Wenwen Ju, Xiaobing Li
miR-205 regulates bone turnover in elderly female patients with type 2 diabetes mellitus through targeted inhibition of Runx2
Exp Ther Med. 2020 Aug;20(2):1557-1565.;doi: 10.3892/etm.2020.8867. (IF 1.785)

8. Bei-Bei Deng, Bao-Ping Jiao, Yang-Jun Liu, Yan-Rong Li, Gui-Jun Wang
BIX-01294 enhanced chemotherapy effect in gastric cancer by inducing GSDME-mediated pyroptosis
Cell Biol Int. 2020 Sep;44(9):1890-1899.;doi: 10.1002/cbin.11395. (IF 2.571)

9. Sen Wang, Xiaomin Hao, Sai He, Changli Liu, Qilong Wang
Suppressive effects of RASAL2 on renal cell carcinoma via SOX2/ERK/p38 MAPK pathway
Exp Ther Med. 2020 Dec;20(6):151.;doi: 10.3892/etm.2020.9280. (IF 1.785)

10. Xiexing Wu, Yijie Liu, Jiacheng Du, Xiaoping Li, Jiayi Lin, Li Ni, Pengfei Zhu, Hong Zhou, Fanchen Kong, Huilin Yang, Dechun Geng, Haiqing Mao
Melatonin Attenuates Intervertebral Disk Degeneration via Maintaining Cartilaginous Endplate Integrity in Rats
Front Physiol. 2021 Jun 14;12:672572.;doi: 10.3389/fphys.2021.672572. (IF 3.367)

生物素高效标记山羊抗小鼠IgG(H+L)(A0288)

生物素高效标记山羊抗小鼠IgG(H+L)
生物素高效标记山羊抗小鼠IgG(H+L)(A0288) 一键复制产品信息

产品编号: A0288

产品包装:0.5ml
选择包装

0.5ml

说明书下载

265.00 256.00 10

价格: ¥ 265.00

促销价: ¥ 265.00

促销价: ¥ 265.00 256.00

产品简介
使用说明
产品文件
相关产品
相关论文
产品问答
产品编号 产品名称 产品包装 产品价格
A0288 生物素高效标记山羊抗小鼠IgG(H+L) 0.5ml 265.00元

来源 用途 WB ICC/IF IHC ELISA
Goat WB, ICC, IF, IHC, ELISA 1:200-2000 1:50-200 1:50-200 1:2000-30000

WB, Western blot; ICC, Immunocytochemistry; IF, Immunofluorescence; IHC, Immunohistochemistry; ELISA, Enzyme-linked immune-sorbent assay.
本生物素高效标记山羊抗小鼠IgG(H+L) (Biotin-labeled Goat Anti-mouse IgG(H+L) with high mole ratio)为进口分装,用于Western、免疫细胞化学、免疫荧光、免疫组化和ELISA等检测的高纯度高特异性二抗。
生物素标记后可以采用SABC(Streptavidin-Biotin Complex)法进行检测;也可以使用辣根过氧化物酶标记的Streptavidin或碱性磷酸酯酶标记的Streptavidin来识别生物素,后续用辣根过氧化物酶或碱性磷酸酯酶对应的检测体系进行显色反应或发光反应等。
本抗体为用纯化的小鼠IgG(H+L)免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过兔IgG等吸附纯化和生物标记的优质二抗。对兔等的血清蛋白几乎没有结合能力(Minimal Cross-reaction to Rabbit)。
本生物素高效标记山羊抗小鼠IgG(H+L)每摩尔IgG结合多摩尔的生物素,能结合多个streptavidin或avidin分子,在SABC或ABC等检测系统中,能有效放大信号,提高检测灵敏度。
本生物素高效标记山羊抗小鼠IgG(H+L)用于Western、免疫细胞化学、免疫荧光、免疫组化和ELISA的推荐稀释比例参考上表,实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。
本抗体如果用于常规的Western检测,以每次检测需10毫升1:1000稀释的二抗计算,可以检测50次;如果用于常规的免疫细胞化学或免疫组化检测,按照每样需要100微升1:100稀释的本抗体计算,可以检测500个样品。
包装清单:

产品编号 产品名称 包装
A0288 生物素高效标记山羊抗小鼠IgG(H+L) 0.5ml
说明书 1份

保存条件:
-20℃保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。 

使用说明:
1. Western、免疫细胞化学、免疫荧光、免疫组化和ELISA请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。

相关产品:

产品编号 产品名称 包装
A0277 生物素标记山羊抗兔IgG(H+L) 1ml
A0279 生物素高效标记山羊抗兔IgG(H+L) 0.5ml
A0286 生物素标记山羊抗小鼠IgG(H+L) 1ml
A0288 生物素高效标记山羊抗小鼠IgG(H+L) 0.5ml
A0303 辣根过氧化物酶标记Streptavidin 0.2ml
A0312 碱性磷酸酯酶标记Streptavidin 0.2ml
P0100A 内源性过氧化物酶封闭液 100ml
P0100B 内源性过氧化物酶强力封闭液 100ml
P0100C 内源性碱性磷酸酶封闭液 6ml
P0101 生物素检测封闭试剂盒 200次
P0603 SABC-HRP Kit (IHC, ICC, Blotting & ELISA) 1000次
P0606 SABC-AP Kit (IHC, ICC, Blotting & ELISA) 1000次
P0612 SABC-HRP Kit with Anti-Mouse IgG (IHC & ICC) 500次
P0615 SABC-HRP Kit with Anti-Rabbit IgG (IHC & ICC) 500次
P0625 SABC-AP Kit with Anti-Mouse IgG (IHC & ICC) 500次
P0628 SABC-AP Kit with Anti-Rabbit IgG (IHC & ICC) 500次

生物素高效标记山羊抗小鼠IgG(H+L)(A0288)
生物素高效标记山羊抗小鼠IgG(H+L)(A0288)

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生物素高效标记山羊抗小鼠IgG(H+L)(A0288)

生物素高效标记山羊抗小鼠IgG(H+L)(A0288)
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生物素标记山羊抗小鼠IgG(H+L)(A0286)

生物素标记山羊抗小鼠IgG(H+L)
生物素标记山羊抗小鼠IgG(H+L)(A0286) 一键复制产品信息

产品编号: A0286

产品包装:1ml
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1ml

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328.00 317.00 10

价格: ¥ 328.00

促销价: ¥ 328.00

促销价: ¥ 328.00 317.00

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产品编号 产品名称 产品包装 产品价格
A0286 生物素标记山羊抗小鼠IgG(H+L) 1ml 328.00元

来源 用途 WB/ELISA IHC/IC IF/F
Goat WB, ELISA, IHC, IC, IF, F 1:1000-3000 1:50-500 1:25-250

WB,Western blot; IHC,Immunohistochemistry; IC,Immunocytochemistry; IF,Immunofluorescence; F,Flow cytometry。
本生物素标记山羊抗小鼠IgG(H+L)(Biotin-labeled Goat Anti-Mouse IgG(H+L))为进口分装,用于Western、ELISA、免疫组化、免疫细胞化学、免疫荧光和流式细胞仪分析的高纯度高特异性二抗。
生物素标记后可以采用辣根过氧化物酶标记的Streptavidin或碱性磷酸酯酶标记的Streptavidin进行后续的检测。随后用辣根过氧化物酶或碱性磷酸酯酶对应的检测体系进行显色反应或发光反应等。
本抗体为用纯化的小鼠IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、牛IgG、马IgG、兔IgG和猪IgG吸附纯化的优质二抗。对人、牛、马、兔和猪的血清蛋白几乎没有结合能力(Minimal Cross-reaction to Human, Bovine, Horse, Rabbit and Swine Serum Proteins)。
本生物素标记山羊抗小鼠IgG(H+L)用于Western、ELISA、免疫组化、免疫细胞化学、免疫荧光和流式细胞仪分析的推荐稀释比例参考上表,实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。
本抗体如果用于常规的Western检测,以每次检测需10毫升1:1000稀释的二抗计,可以检测100次。
包装清单:

产品编号 产品名称 包装
A0286 生物素标记山羊抗小鼠IgG (H+L) 1ml
说明书 1份

保存条件:
-20℃保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. Western、ELISA、免疫组化、免疫细胞化学、免疫荧光和流式细胞仪分析请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。

生物素标记山羊抗小鼠IgG(H+L)(A0286)
生物素标记山羊抗小鼠IgG(H+L)(A0286)

物质安全数据单
输入右侧验证码点击下载:

生物素标记山羊抗小鼠IgG(H+L)(A0286)

生物素标记山羊抗小鼠IgG(H+L)(A0286)
质检证书
输入批号搜寻COA

搜索

相关产品请点击如下按钮:
Biotin标记二抗

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