标签归档：序列

pCMV-VSV-G 载体序列

发表于由

 pCMV-VSV-G 载体序列

型号 载体名称 出品公司 载体用途
VMA0369 pCMV-VSV-G Addgene 慢病毒载体
产品参数: 

5′ sequencing primer: T7

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.

Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus （VSV-G） under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus（MMLV）-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipidcomponents of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein. 
The vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria.

质粒图谱: 

pShuttle-CMV载体序列说明书

发表于由

 

pShuttle-CMV

型号 载体名称 出品公司 载体用途
VXS0388 pShuttle-CMV Stratagene 腺病毒系统
产品参数: 

Size （bp）: 7452
5′ sequencing primer: CMV-F

载体抗性: 
卡那霉素（Kanamycin）
载体描述: 

Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired.
Max insert size = 6.6kb with pAdEasy®1 and 9.3kb with pAdEasy®2.

质粒图谱: 

pCMV-VSV-G载体序列

发表于由

 pCMV-VSV-G载体序列说明书

型号 载体名称 出品公司 载体用途
VMA0369 pCMV-VSV-G Addgene 慢病毒载体
产品参数: 

5′ sequencing primer: T7

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

Vector for constitutive expression of VSV-G glycoprotein. Also known as pHCMV-G or pHCMV-VSV-G.

Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. pCMV-VSV-G expresses the G glycoprotein of the vesicular stomatitis virus （VSV-G） under the control of the CMV immediate-early promoter. VSV-G is used in pseudotyping of Moloney Murine Leukenia Virus（MMLV）-based retroviral vectors by mediating viral entry. VSV-G interacts with phospholipidcomponents of the target cell membrane and fosters the fusion of viral and cellular membranes. VSV-G does not require a cell surface receptor and can serve as a surrogate viral envelope protein. 
The vector contains the ampicillin-resistance gene for propagation and antibiotic selection in bacteria.

质粒图谱: 

CleanCap Cas9 mRNA开放阅读框序列

发表于由

 

CleanCap Cas9 mRNA开放阅读框序列
TriLink产品：
L-7206（用5-甲氧基-U取代）https://www.trilinkbiotech.com/cleancap-cas9-mrna-5mou.html
L-7606（野生型）https://www.trilinkbiotech.com/cleancap-cas9-mrna.html
mRNA可从TriLink BioTechnologies，LLC购买。有关更多信息，请联系sales@trilinkbiotech.com。
此处包含的序列是TriLink BioTechnologies，LLC的机密信息，仅供客户使用。

1 AUGGCCCCCA AGAAGAAGCG GAAGGUGGGC AUCCACGGCG UGCCCGCCGC CGACAAGAAG UACAGCAUCG GCCUGGACAU CGGCACCAAC AGCGUGGGCU 101 GGGCCGUGAU CACCGACGAG UACAAGGUGC CCAGCAAGAA GUUCAAGGUG CUGGGCAACA CCGACCGGCA CAGCAUCAAG AAGAACCUGA UCGGCGCCCU 201 GCUGUUCGAC AGCGGCGAGA CCGCCGAGGC CACCCGGCUG AAGCGGACCG CCCGGCGGCG GUACACCCGG CGGAAGAACC GGAUCUGCUA CCUGCAGGAG 301 AUCUUCAGCA ACGAGAUGGC CAAGGUGGAC GACAGCUUCU UCCACCGGCU GGAGGAGAGC UUCCUGGUGG AGGAGGACAA GAAGCACGAG CGGCACCCCA 401 UCUUCGGCAA CAUCGUGGAC GAGGUGGCCU ACCACGAGAA GUACCCCACC AUCUACCACC UGCGGAAGAA GCUGGUGGAC AGCACCGACA AGGCCGACCU 501 GCGGCUGAUC UACCUGGCCC UGGCCCACAU GAUCAAGUUC CGGGGCCACU UCCUGAUCGA GGGCGACCUG AACCCCGACA ACAGCGACGU GGACAAGCUG 601 UUCAUCCAGC UGGUGCAGAC CUACAACCAG CUGUUCGAGG AGAACCCCAU CAACGCCAGC GGCGUGGACG CCAAGGCCAU CCUGAGCGCC CGGCUGAGCA 701 AGAGCCGGCG GCUGGAGAAC CUGAUCGCCC AGCUGCCCGG CGAGAAGAAG AACGGCCUGU UCGGCAACCU GAUCGCCCUG AGCCUGGGCC UGACCCCCAA 801 CUUCAAGAGC AACUUCGACC UGGCCGAGGA CGCCAAGCUG CAGCUGAGCA AGGACACCUA CGACGACGAC CUGGACAACC UGCUGGCCCA GAUCGGCGAC 901 CAGUACGCCG ACCUGUUCCU GGCCGCCAAG AACCUGAGCG ACGCCAUCCU GCUGAGCGAC AUCCUGCGGG UGAACACCGA GAUCACCAAG GCCCCCCUGA1001 GCGCCAGCAU GAUCAAGCGG UACGACGAGC ACCACCAGGA CCUGACCCUG CUGAAGGCCC UGGUGCGGCA GCAGCUGCCC GAGAAGUACA AGGAGAUCUU1101 CUUCGACCAG AGCAAGAACG GCUACGCCGG CUACAUCGAC GGCGGCGCCA GCCAGGAGGA GUUCUACAAG UUCAUCAAGC CCAUCCUGGA GAAGAUGGAC1201 GGCACCGAGG AGCUGCUGGU GAAGCUGAAC CGGGAGGACC UGCUGCGGAA GCAGCGGACC UUCGACAACG GCAGCAUCCC CCACCAGAUC CACCUGGGCG1301 AGCUGCACGC CAUCCUGCGG CGGCAGGAGG ACUUCUACCC CUUCCUGAAG GACAACCGGG AGAAGAUCGA GAAGAUCCUG ACCUUCCGGA UCCCCUACUA1401 CGUGGGCCCC CUGGCCCGGG GCAACAGCCG GUUCGCCUGG AUGACCCGGA AGAGCGAGGA GACCAUCACC CCCUGGAACU UCGAGGAGGU GGUGGACAAG1501 GGCGCCAGCG CCCAGAGCUU CAUCGAGCGG AUGACCAACU UCGACAAGAA CCUGCCCAAC GAGAAGGUGC UGCCCAAGCA CAGCCUGCUG UACGAGUACU1601 UCACCGUGUA CAACGAGCUG ACCAAGGUGA AGUACGUGAC CGAGGGCAUG CGGAAGCCCG CCUUCCUGAG CGGCGAGCAG AAGAAGGCCA UCGUGGACCU1701 GCUGUUCAAG ACCAACCGGA AGGUGACCGU GAAGCAGCUG AAGGAGGACU ACUUCAAGAA GAUCGAGUGC UUCGACAGCG UGGAGAUCAG CGGCGUGGAG1801 GACCGGUUCA ACGCCAGCCU GGGCACCUAC CACGACCUGC UGAAGAUCAU CAAGGACAAG GACUUCCUGG ACAACGAGGA GAACGAGGAC AUCCUGGAGG1901 ACAUCGUGCU GACCCUGACC CUGUUCGAGG ACCGGGAGAU GAUCGAGGAG CGGCUGAAGA CCUACGCCCA CCUGUUCGAC GACAAGGUGA UGAAGCAGCU2001 GAAGCGGCGG CGGUACACCG GCUGGGGCCG GCUGAGCCGG AAGCUGAUCA ACGGCAUCCG GGACAAGCAG AGCGGCAAGA CCAUCCUGGA CUUCCUGAAG2101 AGCGACGGCU UCGCCAACCG GAACUUCAUG CAGCUGAUCC ACGACGACAG CCUGACCUUC AAGGAGGACA UCCAGAAGGC CCAGGUGAGC GGCCAGGGCG2201 ACAGCCUGCA CGAGCACAUC GCCAACCUGG CCGGCAGCCC CGCCAUCAAG AAGGGCAUCC UGCAGACCGU GAAGGUGGUG GACGAGCUGG UGAAGGUGAU2301 GGGCCGGCAC AAGCCCGAGA ACAUCGUGAU CGAGAUGGCC CGGGAGAACC AGACCACCCA GAAGGGCCAG AAGAACAGCC GGGAGCGGAU GAAGCGGAUC2401 GAGGAGGGCA UCAAGGAGCU GGGCAGCCAG AUCCUGAAGG AGCACCCCGU GGAGAACACC CAGCUGCAGA ACGAGAAGCU GUACCUGUAC UACCUGCAGA2501 ACGGCCGGGA CAUGUACGUG GACCAGGAGC UGGACAUCAA CCGGCUGAGC GACUACGACG UGGACCACAU CGUGCCCCAG AGCUUCCUGA AGGACGACAG2601 CAUCGACAAC AAGGUGCUGA CCCGGAGCGA CAAGAACCGG GGCAAGAGCG ACAACGUGCC CAGCGAGGAG GUGGUGAAGA AGAUGAAGAA CUACUGGCGG2701 CAGCUGCUGA ACGCCAAGCU GAUCACCCAG CGGAAGUUCG ACAACCUGAC CAAGGCCGAG CGGGGCGGCC UGAGCGAGCU GGACAAGGCC GGCUUCAUCA2801 AGCGGCAGCU GGUGGAGACC CGGCAGAUCA CCAAGCACGU GGCCCAGAUC CUGGACAGCC GGAUGAACAC CAAGUACGAC GAGAACGACA AGCUGAUCCG2901 GGAGGUGAAG GUGAUCACCC UGAAGAGCAA GCUGGUGAGC GACUUCCGGA AGGACUUCCA GUUCUACAAG GUGCGGGAGA UCAACAACUA CCACCACGCC3001 CACGACGCCU ACCUGAACGC CGUGGUGGGC ACCGCCCUGA UCAAGAAGUA CCCCAAGCUG GAGAGCGAGU UCGUGUACGG CGACUACAAG GUGUACGACG3101 UGCGGAAGAU GAUCGCCAAG AGCGAGCAGG AGAUCGGCAA GGCCACCGCC AAGUACUUCU UCUACAGCAA CAUCAUGAAC UUCUUCAAGA CCGAGAUCAC3201 CCUGGCCAAC GGCGAGAUCC GGAAGCGGCC CCUGAUCGAG ACCAACGGCG AGACCGGCGA GAUCGUGUGG GACAAGGGCC GGGACUUCGC CACCGUGCGG3301 AAGGUGCUGA GCAUGCCCCA GGUGAACAUC GUGAAGAAGA CCGAGGUGCA GACCGGCGGC UUCAGCAAGG AGAGCAUCCU GCCCAAGCGG AACAGCGACA3401 AGCUGAUCGC CCGGAAGAAG GACUGGGACC CCAAGAAGUA CGGCGGCUUC GACAGCCCCA CCGUGGCCUA CAGCGUGCUG GUGGUGGCCA AGGUGGAGAA3501 GGGCAAGAGC AAGAAGCUGA AGAGCGUGAA GGAGCUGCUG GGCAUCACCA UCAUGGAGCG GAGCAGCUUC GAGAAGAACC CCAUCGACUU CCUGGAGGCC3601 AAGGGCUACA AGGAGGUGAA GAAGGACCUG AUCAUCAAGC UGCCCAAGUA CAGCCUGUUC GAGCUGGAGA ACGGCCGGAA GCGGAUGCUG GCCAGCGCCG3701 GCGAGCUGCA GAAGGGCAAC GAGCUGGCCC UGCCCAGCAA GUACGUGAAC UUCCUGUACC UGGCCAGCCA CUACGAGAAG CUGAAGGGCA GCCCCGAGGA3801 CAACGAGCAG AAGCAGCUGU UCGUGGAGCA GCACAAGCAC UACCUGGACG AGAUCAUCGA GCAGAUCAGC GAGUUCAGCA AGCGGGUGAU CCUGGCCGAC3901 GCCAACCUGG ACAAGGUGCU GAGCGCCUAC AACAAGCACC GGGACAAGCC CAUCCGGGAG CAGGCCGAGA ACAUCAUCCA CCUGUUCACC CUGACCAACC4001 UGGGCGCCCC CGCCGCCUUC AAGUACUUCG ACACCACCAU CGACCGGAAG CGGUACACCA GCACCAAGGA GGUGCUGGAC GCCACCCUGA UCCACCAGAG4101 CAUCACCGGC CUGUACGAGA CCCGGAUCGA CCUGAGCCAG CUGGGCGGCG ACAGCGGCGG CAAGCGGCCC GCCGCCACCA AGAAGGCCGG CCAGGCCAAG4201 AAGAAGAAGG GCAGCUACCC CUACGACGUG CCCGACUACG CCUGA     ...AUGGCCCCCAAGAAGAAGCGGAAGGUGGGCAUCCACGGCGUGCCCGCCGCCGACAAGAAGUACAGCAUCGGCCUGGACAUCGGCACCAACAGCGUGGGCUGGGCCGUGAUCACCGACGAGUACAAGGUGCCCAGCAAGAAGUUCAAGGUGCUGGGCAACACCGACCGGCACAGCAUCAAGAAGAACCUGAUCGGCGCCCUGCUGUUCGACAGCGGCGAGACCGCCGAGGCCACCCGGCUGAAGCGGACCGCCCGGCGGCGGUACACCCGGCGGAAGAACCGGAUCUGCUACCUGCAGGAGAUCUUCAGCAACGAGAUGGCCAAGGUGGACGACAGCUUCUUCCACCGGCUGGAGGAGAGCUUCCUGGUGGAGGAGGACAAGAAGCACGAGCGGCACCCCAUCUUCGGCAACAUCGUGGACGAGGUGGCCUACCACGAGAAGUACCCCACCAUCUACCACCUGCGGAAGAAGCUGGUGGACAGCACCGACAAGGCCGACCUGCGGCUGAUCUACCUGGCCCUGGCCCACAUGAUCAAGUUCCGGGGCCACUUCCUGAUCGAGGGCGACCUGAACCCCGACAACAGCGACGUGGACAAGCUGUUCAUCCAGCUGGUGCAGACCUACAACCAGCUGUUCGAGGAGAACCCCAUCAACGCCAGCGGCGUGGACGCCAAGGCCAUCCUGAGCGCCCGGCUGAGCAAGAGCCGGCGGCUGGAGAACCUGAUCGCCCAGCUGCCCGGCGAGAAGAAGAACGGCCUGUUCGGCAACCUGAUCGCCCUGAGCCUGGGCCUGACCCCCAACUUCAAGAGCAACUUCGACCUGGCCGAGGACGCCAAGCUGCAGCUGAGCAAGGACACCUACGACGACGACCUGGACAACCUGCUGGCCCAGAUCGGCGACCAGUACGCCGACCUGUUCCUGGCCGCCAAGAACCUGAGCGACGCCAUCCUGCUGAGCGACAUCCUGCGGGUGAACACCGAGAUCACCAAGGCCCCCCUGAGCGCCAGCAUGAUCAAGCGGUACGACGAGCACCACCAGGACCUGACCCUGCUGAAGGCCCUGGUGCGGCAGCAGCUGCCCGAGAAGUACAAGGAGAUCUUCUUCGACCAGAGCAAGAACGGCUACGCCGGCUACAUCGACGGCGGCGCCAGCCAGGAGGAGUUCUACAAGUUCAUCAAGCCCAUCCUGGAGAAGAUGGACGGCACCGAGGAGCUGCUGGUGAAGCUGAACCGGGAGGACCUGCUGCGGAAGCAGCGGACCUUCGACAACGGCAGCAUCCCCCACCAGAUCCACCUGGGCGAGCUGCACGCCAUCCUGCGGCGGCAGGAGGACUUCUACCCCUUCCUGAAGGACAACCGGGAGAAGAUCGAGAAGAUCCUGACCUUCCGGAUCCCCUACUACGUGGGCCCCCUGGCCCGGGGCAACAGCCGGUUCGCCUGGAUGACCCGGAAGAGCGAGGAGACCAUCACCCCCUGGAACUUCGAGGAGGUGGUGGACAAGGGCGCCAGCGCCCAGAGCUUCAUCGAGCGGAUGACCAACUUCGACAAGAACCUGCCCAACGAGAAGGUGCUGCCCAAGCACAGCCUGCUGUACGAGUACUUCACCGUGUACAACGAGCUGACCAAGGUGAAGUACGUGACCGAGGGCAUGCGGAAGCCCGCCUUCCUGAGCGGCGAGCAGAAGAAGGCCAUCGUGGACCUGCUGUUCAAGACCAACCGGAAGGUGACCGUGAAGCAGCUGAAGGAGGACUACUUCAAGAAGAUCGAGUGCUUCGACAGCGUGGAGAUCAGCGGCGUGGAGGACCGGUUCAACGCCAGCCUGGGCACCUACCACGACCUGCUGAAGAUCAUCAAGGACAAGGACUUCCUGGACAACGAGGAGAACGAGGACAUCCUGGAGGACAUCGUGCUGACCCUGACCCUGUUCGAGGACCGGGAGAUGAUCGAGGAGCGGCUGAAGACCUACGCCCACCUGUUCGACGACAAGGUGAUGAAGCAGCUGAAGCGGCGGCGGUACACCGGCUGGGGCCGGCUGAGCCGGAAGCUGAUCAACGGCAUCCGGGACAAGCAGAGCGGCAAGACCAUCCUGGACUUCCUGAAGAGCGACGGCUUCGCCAACCGGAACUUCAUGCAGCUGAUCCACGACGACAGCCUGACCUUCAAGGAGGACAUCCAGAAGGCCCAGGUGAGCGGCCAGGGCGACAGCCUGCACGAGCACAUCGCCAACCUGGCCGGCAGCCCCGCCAUCAAGAAGGGCAUCCUGCAGACCGUGAAGGUGGUGGACGAGCUGGUGAAGGUGAUGGGCCGGCACAAGCCCGAGAACAUCGUGAUCGAGAUGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACAGCCGGGAGCGGAUGAAGCGGAUCGAGGAGGGCAUCAAGGAGCUGGGCAGCCAGAUCCUGAAGGAGCACCCCGUGGAGAACACCCAGCUGCAGAACGAGAAGCUGUACCUGUACUACCUGCAGAACGGCCGGGACAUGUACGUGGACCAGGAGCUGGACAUCAACCGGCUGAGCGACUACGACGUGGACCACAUCGUGCCCCAGAGCUUCCUGAAGGACGACAGCAUCGACAACAAGGUGCUGACCCGGAGCGACAAGAACCGGGGCAAGAGCGACAACGUGCCCAGCGAGGAGGUGGUGAAGAAGAUGAAGAACUACUGGCGGCAGCUGCUGAACGCCAAGCUGAUCACCCAGCGGAAGUUCGACAACCUGACCAAGGCCGAGCGGGGCGGCCUGAGCGAGCUGGACAAGGCCGGCUUCAUCAAGCGGCAGCUGGUGGAGACCCGGCAGAUCACCAAGCACGUGGCCCAGAUCCUGGACAGCCGGAUGAACACCAAGUACGACGAGAACGACAAGCUGAUCCGGGAGGUGAAGGUGAUCACCCUGAAGAGCAAGCUGGUGAGCGACUUCCGGAAGGACUUCCAGUUCUACAAGGUGCGGGAGAUCAACAACUACCACCACGCCCACGACGCCUACCUGAACGCCGUGGUGGGCACCGCCCUGAUCAAGAAGUACCCCAAGCUGGAGAGCGAGUUCGUGUACGGCGACUACAAGGUGUACGACGUGCGGAAGAUGAUCGCCAAGAGCGAGCAGGAGAUCGGCAAGGCCACCGCCAAGUACUUCUUCUACAGCAACAUCAUGAACUUCUUCAAGACCGAGAUCACCCUGGCCAACGGCGAGAUCCGGAAGCGGCCCCUGAUCGAGACCAACGGCGAGACCGGCGAGAUCGUGUGGGACAAGGGCCGGGACUUCGCCACCGUGCGGAAGGUGCUGAGCAUGCCCCAGGUGAACAUCGUGAAGAAGACCGAGGUGCAGACCGGCGGCUUCAGCAAGGAGAGCAUCCUGCCCAAGCGGAACAGCGACAAGCUGAUCGCCCGGAAGAAGGACUGGGACCCCAAGAAGUACGGCGGCUUCGACAGCCCCACCGUGGCCUACAGCGUGCUGGUGGUGGCCAAGGUGGAGAAGGGCAAGAGCAAGAAGCUGAAGAGCGUGAAGGAGCUGCUGGGCAUCACCAUCAUGGAGCGGAGCAGCUUCGAGAAGAACCCCAUCGACUUCCUGGAGGCCAAGGGCUACAAGGAGGUGAAGAAGGACCUGAUCAUCAAGCUGCCCAAGUACAGCCUGUUCGAGCUGGAGAACGGCCGGAAGCGGAUGCUGGCCAGCGCCGGCGAGCUGCAGAAGGGCAACGAGCUGGCCCUGCCCAGCAAGUACGUGAACUUCCUGUACCUGGCCAGCCACUACGAGAAGCUGAAGGGCAGCCCCGAGGACAACGAGCAGAAGCAGCUGUUCGUGGAGCAGCACAAGCACUACCUGGACGAGAUCAUCGAGCAGAUCAGCGAGUUCAGCAAGCGGGUGAUCCUGGCCGACGCCAACCUGGACAAGGUGCUGAGCGCCUACAACAAGCACCGGGACAAGCCCAUCCGGGAGCAGGCCGAGAACAUCAUCCACCUGUUCACCCUGACCAACCUGGGCGCCCCCGCCGCCUUCAAGUACUUCGACACCACCAUCGACCGGAAGCGGUACACCAGCACCAAGGAGGUGCUGGACGCCACCCUGAUCCACCAGAGCAUCACCGGCCUGUACGAGACCCGGAUCGACCUGAGCCAGCUGGGCGGCGACAGCGGCGGCAAGCGGCCCGCCGCCACCAAGAAGGCCGGCCAGGCCAAGAAGAAGAAGGGCAGCUACCCCUACGACGUGCCCGACUACGCCUGA

pSicoR载体序列

发表于由

 pSicoR载体说明书

型号 载体名称 出品公司 载体用途
VMA0372 pSicoR Addgene 慢病毒载体
产品参数: 

Backbone size （bp）: 7567
Modifications to Backbone: EGFP is expressed from this plasmid as a marker, but it is not a fusion protein. EGFP and the shRNA oligo are flanked by LoxP sites. Cre causes both to be recombined out of the construct, turning off shRNA expression.
Cloning site 5′: HpaI
Cloning site 3′: XhoI
5′ sequencing primer: mU6-F

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pSicoR allows for conditional （Cre-Lox）, stable expression of shRNAs for RNA interference in cells and transgenic mice. Addition of Cre TURNS OFF shRNA expression.

质粒图谱: 

pAdTrack 载体 序列及说明书

发表于由

 

pAdTrack

型号 载体名称 出品公司 载体用途
VXC0391 pAdTrack Coloncancer 腺病毒系统
产品参数: 

Size （bp）: 8320

载体抗性: 
卡那霉素（Kanamycin）
载体描述: 

Vector pAdTrack is used for expression of transgenes under a chosen （non-CMV） promoter when a GFP tracer is desired.

质粒图谱: