Fluoro-Gel是一种水基封片胶,主要用作组织切片免疫荧光染色后*性封片。由于有些含有甲苯或者二甲苯的封片剂在荧光染色步骤不适合应用,此产品的*配方就发挥了作用,阻止了染色后的一些荧光信号快速变弱的现象。推荐以下荧光染料染色后用本产品封片,FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent Protein (GFP), Tetramethylyrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC)。荧光封片胶封片后建议在暗处4ºC保存。
保存温度 | 货号 | 品名 | 规格 |
2-8ºC | 17985-10 | Fluoro-Gel, (with Tris Buffer) | 20 ml |
2-8ºC | 17985-11 | Fluoro-Gel, (with Tris Buffer) | 100 ml |
2-8ºC | 17985-30 | Fluoro-Gel, (with TES Buffer) | 20 ml |
2-8ºC | 17985-31 | Fluoro-Gel, (with TES Buffer) | 100 ml |
2-8ºC | 17985-40 | Fluoro-Gel, (with PIPE Buffer) | 20 ml |
2-8ºC | 17985-41 | Fluoro-Gel, (with PIPE Buffer) | 100 ml |
二号荧光封片胶Fluoro-Gel II Mounting Medium
与Fluoro-Gel相似(Tris Buffer),不同在于二号产品含有DAPI(4, 6-diamino-2-phenylindole),DAPI是DNA复染的染料。此产品在原位杂交技术或者其他需要DNA荧光染色的技术中经常用到。DAPI发蓝色荧光,RNA同时被染。
货号 | 品名 | 规格 |
17985-50 | Fluoro-Gel II | 20 ml |
17985-51 | Fluoro-Gel II | 100 ml |
三号荧光封片胶Fluoro-Gel III Mounting Medium
Fluoro-Gel III与Fluoro-Gel相似(Tris Buffer),不同在于三号产品加了PI (Propidium Iodide),这也是DNA复染的一种染料。三号产品在以下染料FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa Fluoro 488, Alexa Fluoro 594, Green Fluorescent protein (GFP), Tetramethylyrhodamine, Redox, Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC)。本产品不含Phynylenediamine,Phynylenediamine极容易破坏Cy dyes, RP-E, PC, and APC的免疫荧光,PI发红色荧光,RNA同时也被染色。
货号 | 品名 | 规格 |
17985-60 | Fluoro-Gel III | 20 ml |
17985-61 | Fluoro-Gel III | 100 ml |