AbboMax2020年免疫组化抗体新产品

AbboMax Inc是一种的抗体,多肽和分析产品和服务提供商。作为一家以研究为导向,以客户为中心的公司,AbboMax致力于开发生物产品并提供专业的定制服务,以增加研究领域的价值和改善人们的生活质量

 

Abbomax的科学专业人员一直致力于高性能的研究、开发和制造。
免疫分析试剂多年。我们向生命科学领域的科学家提供高质量的产品。我们是
是许多先进的研究机构和临床诊断公司值得信赖和信赖的供应商。
在我们自己的硅谷核心工厂,我们的产品90%以上都是原装的,

Immunohistochemistry (IHC) Antibodies

AbboMax2020年免疫组化抗体新产品

免疫组织化学(IHC)应用的一级抗体

 

4E-BP1
Cat #: 604-010
克隆:A7J
宿主:小鼠
抗体类型:单克隆
同型:小鼠igg1
交叉反应性:hu,ms

 

乙酰辅酶A Acetyl-CoA
货号:604-030
克隆:A3H
宿主:小鼠
抗体类型:单克隆
同型:小鼠igg1
交叉反应性:Hu

 

ATF2
货号:604-070
克隆:A7D
宿主:小鼠
抗体类型:单克隆
同型:小鼠igg1
交叉反应性:Hu

 

 

BCL—2A
货号:500—1124
克隆:ABM117
宿主:小鼠
抗体类型:单克隆
同型:小鼠igg1
交叉反应性:Hu

 

 

B-RAF
货号:604-110
克隆:A9J
宿主:小鼠
抗体类型:单克隆
同型:小鼠igg1/k
交叉反应性:Hu

 

CCDC6(VEGFR2)
货号:604-120
克隆:A9C
宿主:小鼠
抗体类型:单克隆
同型:小鼠igg1
交叉反应性:hu,ms

 

CD15
货号:500—120
克隆:ABM119
宿主:小鼠
抗体类型:单克隆
同型:小鼠IgM
交叉反应性:Hu

 

CD43
货号:603-990
克隆:10D43A
宿主:小鼠
抗体类型:单克隆
同型:小鼠igg1
交叉反应性:Hu

 

CD44
货号:604-230
克隆:A7G
宿主:小鼠
抗体类型:单克隆
同型:小鼠igg1
交叉反应性:Hu

 

 

4E-BP1          604-010        Mouse monoclonal     A7J    100 ug     Hu, Ms

Acetyl-CoA      604-030        Mouse monoclonal      A3H    100 ug     Hu

ATF2             604-070       Mouse monoclonal      A7D     100 ug     Hu

Bcl-2a            500-1124     Mouse monoclonal     ABM117    100 ug     Hu

B-raf            604-110        Mouse monoclonal      A9J      100 ug     Hu

CCDC6(VEGFR2)     604-120         Mouse monoclonal    A9C        100 ug    Hu, Ms

CD15              500-1204       Mouse monoclonal      ABM119     100 ug    Hu

CD43              603-990            Mouse monoclonal  10D43A      100 ug    Hu

CD44              604-230           Mouse monoclonal        A7G     100 ug     Hu

CEA                 603-070            Mouse monoclonal    ABM330  100 ug    Hu

c-Myc            603-080              Mouse monoclonal      9B7     100 ug  Hu

EGFR           500-6694              Mouse monoclonal       ABM341   100 ug   Hu

HPV 16         500-5834                 Mouse monoclonal    ABM257    100 ug   Hu

Keratin 5/6/18 500-5934            ouse monoclonal     ABM267  100 ug Hu, Rt, Ms

Keratin 7      500-5964        Mouse monoclonal       ABM270    100 ug Hu, Ms

Ki67           604-240          Mouse monoclonal        C8F      100 ug   Hu

MHC II (HLA-DP and DR)  500-6144  Mouse monoclonal      ABM288   100 ug   Hu

Myosin Smooth Muscle Heavy Chain  500-5244  Mouse monoclonal ABM201 100 ug Hu

PAX6           604-250         Mouse monoclonal         E5B       100 ug    Hu

Prostate Specific Acid Phosphatase (PSAP) 500-6394 Mouse monoclonal ABM312 100 ug Hu, Rt, Ms

Retinoblastoma (RB)   500-6814   Mouse monoclonal    ABM353    100 ug      Hu

TSH                 600-570         Mouse monoclonal   M1A10    100 ug      Hu

Vimentin            603-890        Mouse monoclonal     V9B1     100 ug      Hu

 

 

 

常见免疫组化难题集锦(下)


11、如何才能充分脱蜡?

 (1)蜡不溶于水,如果脱蜡不干净,少许蜡存留于切片上,将会引起染色不均匀、阳性物时隐时现、真假难辨、背景染色增加等。为了解决上述的问题,切片在染色前必须*脱蜡,目前用于脱蜡的试剂主要是二甲苯,因它脱蜡力强,脱蜡时间较短;
(2)脱蜡的时间要根据季节,室温和试剂的新鲜谋面是在不同。如果在夏天,室温较高,脱蜡试剂也新鲜,则脱蜡时间不需很多,3-5分钟就已足够。如果在冬天,室温较低,脱蜡试剂也较陈旧,则脱蜡时间需要延长,10-20分钟或更长。
(3)当天切的切片,烧烤2小时后进行染色,切片带有温度进行脱蜡这将可加速脱蜡的过程,如果预先切好烤好的切片,在染色前,还必须对切片进行加温10-20分钟,然后再行脱蜡,这样脱蜡速度加快,效果魁伟更好。总之,操作时应根据不同的季节,不同的室温,不同的试剂来决定,脱蜡的时间,原则上是要*、干净、*地脱去切片上的蜡。

12、如何zui大限度地降低组织非特异性染色?

(1)缩短一抗/二抗孵育时间、稀释抗体来控制。这是zui重要的一条。
(2)一抗用多克隆抗体易出现非特异性着色,建议试用单克隆抗体看看。
(3)内源性过氧化物酶和生物素在肝脏、肾脏等组织含量很高(含血细胞多的组织),需要通过延长灭活时间和增加灭活剂浓度来降低背景染色;
(4)非特异性组分与抗体结合,这需要通过延长二抗来源的动物免疫血清封闭时间和适当增加浓度来加强封闭效果;
(5)缩短DAB孵育时间或降低DAB浓度/过氧化氢浓度等;
(6)适当增加PBS冲洗次数和浸洗时间,在一抗、二抗或SP孵育之后的浸洗尤为重要;
(7)防止标本染色过程中出现干片,这容易增强非特异性着色。

13、苏木素复染时间的把握?

(1)苏木素复染时间要看当时的室温、溶液的新旧、目标抗原的定位等情况,一般数秒-数分钟。不过这个如果染色不理想可以补救的。即:染色深则分化时间稍长些即可;染色浅则再置于苏木素中染色即可。
(2)盐酸酒精是分化,氨水是返兰。作用不同。片子复染完后流水振洗,然后置于盐酸酒精中数秒(一定动作要快)后拿出流水振洗,在放入氨水中返兰即可。
(3)如果分化的颜色过浅,可以复置于苏木素中染色。

14、PBS的清洗方式选择、次数和时间的选择?

(1)单独冲洗,防止交叉反应造成污染。 临床上每天检测的病例很多,所用的抗体种类及项目也多,如果在加入一抗孵育完后,将它们在一个缸内洗,这样就会造成交叉污染,影响zui后的结果。正确的做法是单独地进行冲洗,冲洗的PBS为一次性,保证切片没有相互交叉污染的机会。
(2)温柔冲洗,防止切片的脱落。冲洗切片,取出切片,将PBS从上轻轻地冲洗,让PBS自上而下流下来,不要拿起切片将PBS对准切片冲洗,这样由于冲出的PBS有一定的冲击力,很容易使切片周边引起松动,导致切片的脱落。
(3)冲洗的时间要足够,才能*洗去结合的物质。冲洗切片有人提出用微振荡器振染,振下未结合的各种物,使之不产生背景,此种做法一是浪费时间,二是很容易导致切片的脱落。切片的冲洗据实践认为,用一小烧杯柔和地于切片上冲洗,就能*冲洗去未结合的物质,无需附加其它条件,冲洗好于切片上再注入PBS,持续2分钟左右就*足够了。
(4)PBS的PH和离子强度的使用和要求。刘彦仿指出:中性及弱硷性条件(PH7-8)有利于免疫复合物的形成,而酸性条件则有利于分解;低离子强度有利于免疫复合物的形成,而高离子强度则有利于分解。[]免疫组化P56我们目前常用的PBS的PH在7.4-7.6浓度是0.01M,根据本室十几年来的使用情况,认为该溶液价格便宜配制方面,使用效果好。
(5)常用试剂的配制和使用。在免疫组织化学的染色过程中,用得zui多的试剂就是缓冲液,因为切片在整个染色中,抗体的加、换、切片的冲洗,DAB的配制都离不开缓冲液。可见缓冲液在整个染色中都起至关键的作用,缓冲液的过酸或偏碱,都将影响染色的结果。

15、脱片产生的原因和如何防止脱片?

 (1)多聚赖氨酸玻片质量的问题。我原先是买的,迈新按说也是不错的,可是都脱成什么样子了。后面补做第二批时用的病理科老师自己做的片子,要好一点。
(2)组织切的不好,切片机的问题例如比较老的旧的机器切的厚或者不均匀,或者切片者手法不好等。
(3)组织的问题,我用的组织癌症的很多,越是癌症组织有坏死之类越容易脱。
(4)没烤好,时间短温度不够之类。
(5)操作的时候甩的太猛了,有脱片嫌疑的片子不甩或轻轻甩,用卫生纸从边缘上慢慢吸水。
(6)修复的问题:抗原修复的时候高压时间过长了,或者放进100度的修复液时手法不好,咚的一声就丢进去了,这样超容易脱片。此外,用EDTA修复比柠檬酸容易脱片,但是你要用到EDTA的时候也没办法,只有从另外的问题上着手。
(7)此外,一旦见到有组织漂起来操作就更加要谨慎,用PBS的时候尽量用泡的,不要冲。基本上把这些方面都注意到了,能改善的尽量改善,脱片可以减少很多。

16、背景染色较深的原因有哪些?

 (1)抗体浓度过高:一抗浓度过高是常见的原因之一。解决办法是,每次使用新抗体前应当对其工作浓度进行测试,使每一抗体个体化,找到适合自己实验室的理想工作浓度,既使是即用型的抗体也应如此,不能只简单的按说明书进行染色。
(2)抗体孵育时间过长或温度较高:解决办法是,严格执行操作规程,随身佩带报时表或报时钟,及时提醒,避免因遗忘而造成时间延长。现在流行的二步法(Polymer)敏感性很高,要求一抗孵育的时间不是传统的1小时,而是30分钟,因此,要根据染色结果进行调整。
(3)DAB变质和显色时间太长:DAB现用现配,如有沉渣应进行过滤后再用。配制好的DAB不应存放时间太长,因为在没有酶的情况下,过氧化氢也会游离出氧原子与DAB产生反应而降低DAB的效力,未用完的DAB存放在冰箱里几天后再用这种似乎节约的办法是不可取的。DAB的显色在显微镜下监控,达到理想的染色程度时立即终止反应。不过当染色片太多时或用染色机时,这样做似乎不现实,但至少应对一些新的或少用的抗体显色时进行监控,避免显色时间过长。
(4)组织变干:修复液溢出后未及时补充液体、染色切片太多、动作太慢、忘记滴液、滴液流失等都是造成组织变干的原因。解决的办法是操作要认真仔细,采用DAKO笔或PAP Pen在组织周围画圈,可以有效的避免液体流失,也能提高操作速度。
(5)切片在缓冲液或修复液中浸泡时间太长(大于24小时):原因上不清楚,但现象存在。有的实验室喜欢前一天将切片脱蜡至修复,第二天加抗体进行免疫组化染色,如果将装有切片和修复液的容器放在4ºC冰箱过夜,对结果无明显影响,如果放在室温,特别是炎热的夏天,会出现背景着色,因此,不可存放时间太长。
(6)一抗变质、质量差的多克隆抗体:注意抗体的有效期,过期的抗体要麽不显色要麽背景着色。用新买抗体时设立阳性对照和用使用过的抗体作比较。

17、苏木素复染后氨水返蓝这一步如何做、浓度多少、时间多长? 

答:返蓝可以用碱性溶液(PBS/Na2HPO4/淡氨水)或45度温水、冷水蓝化均可。一般蓝化5~10 min。淡氨水有人用50ml自来水+三四滴的氢氧化铵。

biosb——免疫组化(IHC)抗体和检测

biosb——免疫组化(IHC)抗体和检测

biosb

链接:www.biosb.com

biosb成立于1998年,位于美国加利福尼亚州,致力于分子病理学研究,研发高品质的组织化学检测产品。biosb提供高质量的人类和动物组织样本、抗体、试剂和诊断试剂等产品,用于科研和诊断领域。该公司的产品主要用于用于免疫组织化学(IHC)、免疫细胞化学(ICC)、免疫荧光(IF)、显色原位杂交(CISH)和荧光原位杂交(FISH)技术涵盖癌症、免疫学、神经科学、糖尿病、心血管和代谢疾病等领域的研究和治疗。biosb的使命是开发和提供高质量的产品,同时为客户提供优质的技术服务。


biosb主要产品线:

一、免疫组织化学产品

免疫组织化学使用抗体、酶偶联物在组织或细胞中定位特定抗原的方法。抗原抗体反应可以在光学显微镜下观察到。抗体可以是多克隆的或单克隆的(通常来自老鼠或兔子)。用于研究和临床实验室检测感染性病原体、癌症其他目标蛋白质。

biosb提供广泛的免疫组织化学产品(IHC),包括用于IVDASR应用的兔和小鼠单克隆抗体和多克隆抗体,以及完整的检测系统或其组件。biosb提供HRPAP试剂盒:ImmunoDetector试剂盒使用传统的三步生物素链霉亲和素酶/底物色原技术,而PolyDetectorPolyDetectorPlus试剂盒是使用专有的串联超标记技术开发的,用于用酶直接标记免疫球蛋白。这确保了在不同类型的组织或细胞中对所有类型的细胞核、细胞质和膜抗原进行一致且可重复的免疫染色。我们还提供用于ER/PRHER-2neuCD117RUO Complete PolyDetector HRP/DAB试剂盒,包括所有试剂、溶液、组织对照和试剂对照

 

二、免疫荧光产品

免疫荧光是一种用于荧光显微镜的光学显微镜技术,主要用于生物样品。该技术利用抗体对其抗原的特异性,同时使用荧光染料在细胞内产生抗原抗体的信号,因此使靶分子在样品中的分布可视化。biosb提供一系列的直接免疫荧光和间接免疫荧光抗体

 

三、荧光原位杂交法(FISH)与显色原位杂交法(CISH

biosb通过与德国ZytoVision的分销合作伙伴关系,提供分子病理学行业最大的CISHFISH产品线之一。包括数百个用于CISHFISH应用的探针和试剂盒。ZytoFast®Zytodot®提供多种ChromegenecCISH)解决方案。此外ZytoLight®产品线提供了100多种单色、双色、三色和四色探针。

 

四、组织和细胞系芯片

正常人组织芯片NH-TMA)、癌症组织芯片CH-TMA)和癌症人细胞系芯片CH-CLMA)是在多个组织上测试抗体、ISH探针或其他试剂的一种方法。

所有产品都已通过免疫组织化学方法进行了验证,并且对100多种抗体呈阳性。

biosb优势:

1.专业研发:Bio SB专注于免疫组织化学(IHC)抗体和检测系统的研发,提供包括IHC、ICC、IF、CISH和FISH技术在内的广泛产品线,满足不同研究和诊断需求。

2.高质量标准:公司产品遵循FDA QSR 21 CFR Part 820 cGMP、CE IVD和ISO 13485:2016等国际认证标准生产,确保了产品的高标准和质量。

3.环境友好:Bio SB提供环保型的辅助设备,体现了公司对环境保护和可持续发展的承诺。

4.创新技术:公司致力于开发独t的IVD验证抗体和检测系统,推动分子病理学领域的技术进步。

5.国际认可:Bio SB的产品和解决方案在全球范围内受到认可,服务于多个国家和地区的科研和医疗市场。

6.以人为本:公司注重团队合作和人才发展,以诚信和热忱的态度完成工作,推动公司创新和成功。

7.社交媒体影响力:Bio SB在LinkedIn等职业社交网络上拥有一定的关注者,显示出其在行业内的社交影响力和认可度。

biosb客户群:

1.医院和诊断实验室:这些机构需要使用Bio SB提供的免疫组织化学(IHC)抗体和检测系统来进行疾病诊断和研究。

2.大学和研究机构:在进行基础科学研究和开发新型治疗方法时,这些学术机构会使用Bio SB的产品。

3.生物技术公司:专注于生物制药和分子诊断的公司可能会采用Bio SB的产品进行药物开发和生物技术研究。

4.制药企业:在开发新药和进行临床试验的过程中,制药企业可能会利用Bio SB的IVD验证抗体和其他分子病理学产品。

5.认证机构:在产品的认证和标准化过程中,可能需要使用Bio SB的高质量标准产品。

6.消费部门:随着生物基产品需求的增长,终端消费者市场也在逐渐成为Bio SB的潜在客户群。

7.全球市场:Bio SB的产品和解决方案在全球范围内受到认可,服务于不同国家和地区的科研和医疗市场。

8.社交媒体关注者:Bio SB在LinkedIn上的1,235名关注者可能包括行业内专业人士、合作伙伴或潜在客户。

 biosb热卖产品

货号

产品名称

规格

品牌

BSB-3739-05

MTAP

0.5 mL

biosb

BSB2224

Survivin (EP119), RMab

7 mL

biosb

BSB-3739-01

MTAP

0.1 mL

biosb

BSB-3728-01

HEG1

0.1 mL

biosb

BSB6866

c-myc

0.1 mL

biosb

BSB 2227

Survivin (EP119), RMab

0.5 mL

biosb

BSB6583

Tinto c-Met/HGFR

3 mL

biosb

BSB6558

Cyclin E1

0.1 mL

biosb

BSB6556

Tinto Cyclin E1

7 mL

biosb

BSB3200

SATB2

0.1 mL

biosb

BSB0128

PolyDetector HRP Green Kit

15 mL

biosb

BSB 2223

Survivin (EP119), RMab

3.0 mL

biosb

BSB-3754-3

Tinto Trop-2/EGP-1

3 mL

biosb

BSB3363

Tinto LAG-3/CD223

3 mL

biosb

BSB6956

MUM1

0.1 mL

biosb

BSB2513

HER2 neu

0.1 mL

biosb

BSB2981

MDM2

0.1 mL

biosb

BSB3282

Helicobacter Pylori (EP279), RMab

0.1 mL

biosb

BSB3283

Helicobacter Pylori (EP279), RMab

0.5 mL

biosb

BSB3284

Helicobacter Pylori (EP279), RMab

1.0 mL

biosb

BSB5270

CD56

0.1 mL

biosb

BSB5642

Herpes Simplex Virus I & II

0.5 mL

biosb

BSB0155

ChromoProtector

500 mL

biosb

BSB6936

MSH6

0.5 mL

biosb

BSB5712

Ki-67

0.5 mL

biosb

BSB6243

Glypican-3

0.1 mL

biosb

BSB6579

c-myc

0.1ml

biosb

BSB6720

EGFR Phospho

0.5 mL

biosb

BSB-0355-5

Mohs PolyDetector Plus DAB HRP Brown Detection System

5 mL

biosb

BSB 0309

Mohs Mouse/Rabbit PolyDetector DAB HRP Brown Detection System

100 mL

biosb

BSB 2225

Tinto Survivin

15 mL

biosb

BSB 3202

SATB2 (EP281), RMab

1 mL

biosb

BSB 5272

CD56 (123C3.D5), MMab

1 mL

biosb

BSB 6320

CD25

0.1 mL

biosb

BSB 6560

Cyclin E1 (EP126), RMab

1.0 mL

biosb

BSB 6581

c-Myc (EP121), RMab

1 mL

biosb

BSB 6868

c-Myc (9E10), MMab

1 mL

biosb

BSB 6958

MUM1 (EP190), RMab

1 mL

biosb

BSB2136

Prolactin

0.1 mL

biosb

BSB-3739-1

MTAP

1 mL

biosb

BSB-3742-05

pan-TRK

0.5 mL

biosb

BSB-3803-3

Tinto Candida Albicans

3 mL

biosb

BSB-7087

TintoRetriever Digital Pressure Cooker Bundle

EA

biosb

BSB-9419-CS

Trop-2/EGP-1

5 Slides

biosb

BSB0077

PolyDetector Alk Magenta

15 mL

biosb

BSB 0307S

Mohs Mouse/Rabbit PolyDetector DAB HRP Brown Detection System

5.0 ml

biosb

BSB2073

p40

0.1 mL

biosb

BSB2123

PMS2

0.5 mL

biosb

USBiological品牌代理

USBiological

简要描述:

Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。

Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,000种抗体、抗原和生化产品,为科研用户提供了诸多 *选择。


We are committed to reducing the cost of research with value, integrity and a truly personal buying experience. We believe in answering all inquiries within a few hours of receipt. You always get to speak with humans (our rabbits are shy). Our technical support department is comprised of scientists with decades of experience. We will promptly and thoroughly answer your inquiries and enjoy helping guide you through protocols whether it be for our products or your personal research.


United States Biological's biochemicals, antibodies, recombinant proteins, cell culture media and molecular biology kits are used in virtually all scientific applications and settings, including genomic research, biotechnology, pharmaceutical development and the diagnosis of disease. Products are available from affordable research quantities to larger bulk amounts for process development and production. Our customers include most of the leading pharmaceutical and biotechnology companies located in the US and abroad.International Customer service and technical support is available in all countries; hundreds of employees and distributors provide excellent service worldwide.


产品列表:


Primary Antibodies

Secondary Antibodies

Isotype Controls

Epitope Tags

Matched Pair Antibody Sets

Custom Antibodies

Amino Acids

Antibiotics & Antimycotics

Drugs & Analogues

Dyes & Stains

Fine Chemicals

Inorganics

Isotopes

Molecular Biology

Phytochemicals

Cells & Lysates

Plasma & Serum

Tissues

Culture Media

Insect Media

Microbiological Media

Molecular Biology Media

Nematode Media

pEX™ Protein Expression

Plant Media

Yeast Media/Drop-out

Cloning

Custom

Enzymes

Glycobiology

Growth Factors/Cytokines

Immunoglobulins

Infectious Disease

Lectins

Peptides

Proteins

Separation Detection

Antibody Sets

Apoptosis

Cell Growth, Axol™

Cloning & PCR

Custom

DNA & RNA Purification

ELISA

Electrochemiluminescence

Immunohistochemistry

Immunoprecipitation

Molecular Biology

Western Blot


Novocastra品牌代理

Novocastra

简要描述:

Novocastra是一家英国公司,成立于1989年,致力于生物医学研究试剂的开发与生产,并不断拓宽免疫组化、免疫荧光、Western blotting、原位杂交等产品。

Novocastra是一家英国公司,成立于1989年,致力于生物医学研究试剂的开发与生产,并不断拓宽免疫组化、免疫荧光、Western blotting、原位杂交等产品。


产品列表:


No.

品牌

货号

名称

规格

1

Novocastra

AKT-PHOS-L-CE

NCL-L-Akt-Phos

1 mL

2

Novocastra

PA0117

Bcl-2 Bond RTU Primary

7 mL

3

Novocastra

CD4-368-L-CE-H

NCL-L-CD4-368

0.5 mL

4

Novocastra

PA0134

CK HMW Bond RTU Primary

7ml

5

Novocastra

MYOTILIN

NCL-MYOTILIN

1ml

6

Novocastra

N-CAD-L-CE

NCL-L-N-Cad

1ml

7

Novocastra

PA0057

p53 Bond RTU Primary

7ml

8

Novocastra

PA0238

Galectin-3 Bond RTU Primary

7ml

9

Novocastra

PA0704

HGH Bond RTU Primary

7ml

10

Novocastra

DRP2-CE

NCL-DRP2

2.5ml

11

Novocastra

PA0776

TSH Bond RTU Primary

7ml

12

Novocastra

ZAP-70-L-CE

NCL-L-ZAP-70

1ml

13

Novocastra

VILLIN-L-A

NCL-L-VILLIN ASR

1ml

14

Novocastra

G-SARC-CE

NCL-g-SARC

1ml 

15

Novocastra

THY-L

NCL-L-THY

1ml

免疫组化——封片剂篇

一 、普通型免疫组化封片剂VectaMount ™Mounting Media

*性免疫组化封片剂VectaMount ™ 
● *性,非水溶性封片剂(样本需经脱水处理)
● 不含甲苯和二甲苯
● 危险性低
● 无特殊气味
● 可与过氧化物酶、碱性磷酸酶、葡萄糖氧化酶显色底物(如DAB, TMB, and BCIP/NBT, a,Vector® NovaRED™, Vector® VIP, Vector® SG, ImmPACT™ DAB, ImmPACT™ DAB EqV, ImmPACT™ NovaRED™, ImmPACT™ VIP, ImmPACT™ SG, Vector® Red, Vector® Blue, and Vector® Black)兼容

产品信息

 产品名称

 产品编号

 规格

 VectaMount Permanent Mounting Medium

 H-5000

 60ml

VectaMount AQ Aqueous Mounting Medium

产品特点
● 水溶性封片剂(样本无需经脱水处理)
● 可与过氧化物酶、碱性磷酸酶、葡萄糖氧化酶显色底物(如AEC, ImmPACT™ AEC, and ImmPACT™ AMEC Red, DAB, Vector® SG, BCIP/NBT, Vector® Red, Vector® Blue, ImmPACT™ DAB, ImmPACT™ DAB EqV, and ImmPACT™ SG)兼容

 

 产品名称

 产品编号

 规格

 VectaMount AQ Aqueous Mounting Medium

 H-5501

 60ml

二 、防荧光淬灭免疫组化封片剂VECTASHIELD Mounting Media for Fluorescence
VECTASHIELD®系列免疫组化封片剂特别适用于防止荧光淬灭。不同形式的VECTASHIELD®封片剂均可很好地起到防荧光淬灭的作用,且可与荧光素, Texas Red®, AMCA, DyLight®染料、Alexa Fluor® 染料、荧光核染料、荧光蛋白、荧光示踪剂、组织化学染料和多数荧光基团兼容。

产品特点
● 可防止多数荧光淬灭
● 提供非硬化和硬化形式
● 提供含或不含DAPI/PI形式
● 无需加温处理
● 切片可长期保存
● 可长期防止封闭的切片发生荧光淬灭
● 使用方便
● 切片清晰
● 封片后折射率佳
 

 荧光染料

 荧光保存率(%)

 DyLight®488 

 92%

 Alexa Fluor®488

 97%

 Fluoresein

 98%

 Alexa Fluor®546

 86%

 DyLight®549

 96%

 Tetramethylrhodamine

 97%

 Cy™3

 94%

 DyLight®594

 96%

 Alexa Fluor®594

 87%

 Texas Red®

 100%

 DyLight®649

 94%

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

*VECTASHIELD® Mounting Medium防荧光淬灭特性
 

 荧光染料

 荧光保存率(%)

 DyLight®488

 100%

 Alexa Fluor®488

 97%

 Fluoresein

 97%

 Alexa Fluor®546

 72%

 DyLight®549

 99%

 Tetramethylrhodamine

 99%

 DyLight®594

 92%

 Alexa Fluor®594

 93%

 Texas Red®

 97%

 DyLight®649

 99%

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

*VECTASHIELD® HardSet™ Mounting Medium防荧光淬灭特性

产品信息

 产品名称  复染剂  产品编号  规格  硬化与否  折光率
 VECTASHIELD®Mounting Medium  none  H-1000  10ml  否  1.44
 DAPI  H-1200  10ml  否  1.44
 PI  H-1300  10ml  否  1.44
 VECTASHIELD®HardSet™ Mounting
 Medium
 none  H-1400  10ml  是  1.46*
 DAPI  H-1500  10ml  是  1.46*

*硬化后检测的折射率

Electron Microscopy Sciences 免疫组化耗材


Electron Microscopy Sciences 免疫组化耗材

简要描述:SEM用导电胶带,镍颗粒嵌在粘结剂中。
总厚度:0 .075 mm (3.0 mil)
粘结剂厚度:0.040mm (1.6 mil)
基底材质:压延铜箔
导电物质:镍粉

详细介绍

产品咨询

Embedding Supplies, Capsules, Molds, and Markers

ProcessingEmbedding Cassettes Systems by Tissue Tek®; Sakura Finetek

1. Tissue-Tek® Process/Embedding Cassette

Made from chemical resistant plastic. These cassettes have an angled, texture writing surface, ensuring positive identification throughout processing, embedding, sectioning and storage. Stainless steel cover is available separay

Cat. No. Tissue-Tek Description Pack Price  
62520-C 4191 Clear 1500/cs 207.00 Add to Cart
62520-W 4187 White 1500/cs 206.00 Add to Cart
62520-A 4129 Aqua 1500/cs 228.00 Add to Cart
62520-B 4184 Blue 1500/cs 228.00 Add to Cart
62520-G 4128 Gold 1500/cs 230.00 Add to Cart
62520-GR 4183 Green 1500/cs 230.00 Add to Cart
62520-O 4126 Orange 1500/cs 230.00 Add to Cart
62520-P 4182 Pink 1500/cs 230.00 Add to Cart
62520-Y 4179 Yellow 1500/cs 230.00 Add to Cart
62520-25 4194 SS Cassette Cover 25/pk 43.00 Add to Cart
2. Tissue-Tek® Embedding Rings

Made from polystyrene and reinforced with the dual bar center support to prevent warping, these rings have a cross-hatch surface design that provide excellent paraffin bonding for clamping and sectioning.

Cat. No. Tissue-Tek Description Pack Price  
62525-W 4151 White 1000/pk 187.00 Add to Cart
62525-B 4116 Blue 1000/pk 187.00 Add to Cart
62525-G 4116 Green 1000/pk 187.00 Add to Cart
62525-P 4116 Pink 1000/pk 187.00 Add to Cart
62525-Y 4116 Yellow 1000/pk 187.00 Add to Cart
3. Tissue-Tek® Base Molds for Embedding Rings

Made from high quality stainless steel for optimal thermal conductivity, and they have a well polished surface for easy paraffin block removal. All of the corners are rounded for good paraffin ribboning. These Base Molds are for all applications in specimen embedding with Tissue-Tek® Embedding Rings shown above.

Cat. No. Tissue-Tek Description Pack Price  
62527-16 4121 Base Mold 16x16x6 12/pk 89.00 Add to Cart
62527-22 4122 Base Mold 22x22x6 12/pk 89.00 Add to Cart
62527-32 4123 Base Mold 32x25x6 12/pk 89.00 Add to Cart
62527-38 4124 Base Mold 38x25x6 12/pk 102.00 Add to Cart
62528-22 4131 Base Mold 22x22x12 12/pk 102.00 Add to Cart
62528-32 4132 Base Mold 32x25x12 12/pk 102.00 Add to Cart
62528-38 4133 Base Mold 38x25x12 12/pk 102.00 Add to Cart

Tissue-Tek® Embedding Supplies; Sakura Finetek

1. Tissue-Tek® Mold Release, Concentrate

Use this solution to coat base molds to ensure complete separation of embedding paraffin from the mold. The working solution is prepared by adding 5 ml of Concentrate to 95 ml of ethyl alcohol or isopropyl alcohol. Molds are dipped in solution and dried prior to use.

2. Tissue-Tek® Marking Pencil

These pencils are made from soft lead, which is ideal for marking plastic cassettes.

Cat. No. Tissue-Tek Description Pack Price  
62530-01 4141 Mold Release 4 oz 9.50 Add to Cart
62530-12 4141 Mold Release 12/cs 108.00 Add to Cart
62531-01 4160 Marking Pencil 12/pk 12.75 Add to Cart
62531-06 4160 Marking Pencil 6pk/cs 68.00 Add to Cart

Tissue-Tek® Cryomold®; Sakura Finetek

These disposable plastic Cryomolds produce a uniformly shaped flat surface specimen block with tissue freezing medium (O.C.T.) for frozen sectioning, Standard Cryomold fits directly into Cryobar® freezing wells of the Tissue-Tek® II microtome/cryostat. Use a Cryomold® adapter to adapt smaller biopsy and intermediate Cryomold® molds to freezing wells on the Cryobar® unit.

Cat. No. Tissue-Tek Description Pack Price  
62534-25 4557 Standard, 25x20x5mm 100/pk 19.50 Add to Cart
62534-10 4565 Biopsy, 10x10x5mm 100/pk 19.50 Add to Cart
62534-15 4566 Intermediate 15x15x5mm 100/pk 19.50 Add to Cart

Tissue-Tek® II Microtome Object Holders; Sakura Finetek

A reusable brass thermal conductive holder with an embossed surface for the Tissue-Tek II microtome/cryostat and for conventional procedures for frozen tissue embedding.

Cat. No. Tissue-Tek Description Pack Price  
62538-01 4560 Solid Object Holder (left image) each 17.50 Add to Cart
62538-06 4560 Solid Object Holder 6/pk 99.00 Add to Cart

Accessories for Tissue-Tek® CRYO3® Microtome/Cryostat Cryo 2000™

1. Cryomold® Specimen Holders

Plastic Cryomold®, round shapes with a tab for easy handling. Available in standard and biopsy configurations. The standard mold measures 22mm (D) x 5mm deep. The Biopsy mold measures 15mm (D) x 5mm deep.

 
Cat. No. Tissue-Tek Description Pack Price  
62532-01 4728 CRYO3 CryoMold, Standard 100/cs 26.00 Add to Cart
62532-12 4728 CRYO3 CryoMold, Standard 1200/cs 218.00 Add to Cart
62533-01 4730 CRYO3 Cryomold, Biopsy 100/cs 26.00 Add to Cart
62533-12 4730 CRYO3 Cryomold, Biopsy 1200/cs 218.00 Add to Cart
2. Round Object Holders

Machined from solid aluminum with an embossed surface for holding frozen embedding blocks. Measures: 26mm top diameter (without ‘O’ ring), 28.55mm diameter with ‘O’ ring, 23mm body diameter, 16mm height .

62537-01 4726 Round Object Holder, 25mm each 32.00 Add to Cart
62537-06 4726 Round Object Holder, 25mm 6/cs 180.00 Add to Cart

Autopsy Baskets and Cassettes

Made from stainless steel. It is fully perforated allowing for free movement of fluids. It comes with a snap-cap which provides positive enclosure.

62310-03 is no longer available. Please see 62320-01.
 

Catalog # Description Measures, mm Pack Price  
62310-12 SS Autopsy Cassettes 50(L)x25(W)x12(D) each 53.00 Add to Cart

Embedding Rings

Molded from the highest quality plastic, our Embedding Rings fit most base molds. For added convenience, there are four colors from which to choose (Blue, Pink, Green, Yellow). The large writing surface further eases identification and handling. The base measures 1 5/8″ x 1 1/8″ and the ring measures 1″ x 1″ (25 x 25 mm).

62350-W Embedding Ring White 250/bx 30.50 Add to Cart
62350-B Embedding Ring, Blue 250/bx 30.50 Add to Cart
62350-P Embedding Ring, Pink 250/bx 30.50 Add to Cart
62350-G Embedding Ring, Green 250/bx 30.50 Add to Cart
62350-Y Embedding Ring, Yellow 250/bx 30.50 Add to Cart

Disposable Base Mold

Our Disposable Base Mold is designed to fit all existing cassettes. Because our base molds are disposable you save time on cleaning and storing and they offer you less exposure to hazardous chemicals.

62352-07 Disposable Base Mold 7x7x5mm 500/pk 68.00 Add to Cart
62352-15 Disposable Base Mold 15x15x5mm 500/pk 68.00 Add to Cart
62352-24 Disposable Base Mold 24x24x5mm 500/pk 68.00 Add to Cart
62352-30 Disposable Base Mold 30x24x5mm 500/pk 68.00 Add to Cart
62352-37 Disposable Base Mold 37x24x5mm 500/pk 68.00 Add to Cart

Paraffin Tissue Microarrays

Paraffin tissue microarrays (TMAs) are becoming more common in the laboratories. Current techniques including organizing, punching, and inserting tissues is time consuming and involving costly equipments.This Arraymold is the simple tool but great answer for this current technique for laboratories to perform TMAs with less time consuming, excellent results.

With this Arraymold, you can process up to 150 specimens onto one single slide with very little time consuming, minimal technical training.

The DVD instruction is accompanying with each kit, will step by step guide you to perform through the entire TMAs process. Arraymold features include:

  • Allow pathologist or researcher to view many different samples on the same single slide
  • Helpful in finding IHC markers for control or quality assurance from hundreds of samples
  • Staining markers will be more consistent when hundreds of samples are on one slide
  • Used less costly antibody
  • Arraymold kit is small enough to be stored in a drawer
  • Rubber arraymold can be reused many times without losing its flexibility.

Ordering:

Arraymold Kit A, (60 cores)

This mold generates 2 mm diameter sample core, 60 cores per mold. Kit consists of:

10 Disposable 2 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 2 mm reusable rubber Arraymold (60 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

69130-10 Arraymold Kit A, 60 Cores each 999.00 Add to Cart
Arraymold Kit B, (150 cores)

This mold generates 1.5 mm diameter sample core, 150 cores per mold. Kit consists of

10 Disposable 2 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 1.5 mm reusable rubber Arraymold (150 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

69130-20 Arraymold Kit B, 150 Cores each 999.00 Add to Cart
Arraymold Kit C, (150 cores)

This mold generates 4 mm diameter sample core, 15 cores per mold. Kit consists of:

10 Disposable 4 mm sterile dermal needle

2 Stylets (used to remove sample from needle)

1 Brass Plate (for setting cryoarray punches)

1 4 mm reusable rubber Arraymold (150 cores)

1 DVD instruction (NTSC) for showing how to use the mold.

69130-40 Arraymold Kit C, 15 Cores each 999.00 Add to Cart
Arraymold Package Kit

To save money, you can order Arraymold Package Kit. This package includes two or three Arraymold kits listed above.

  • Arraymold Kit 1: Including Arraymold Kit A and Arraymold Kit B
  • Arraymold Kit 2: Including Arraymold Kit A and Arraymold Kit C
  • Arraymold Kit 3: Including Arraymold Kit B and Arraymold Kit C
  • Arraymold Kit 4: Including all three Arraymold Kit A, B, and C
69131-01 Arraymold Package Kit 1 (A +B) each 1798.00 Add to Cart
69131-02 Arraymold Package Kit 2 (A +C) each 1798.00 Add to Cart
69131-03 Arraymold Package Kit 2 (B +C) each 1798.00 Add to Cart
69131-04 Arraymold Package Kit 2 (A +B+C) each 2677.00 Add to Cart

Micro Baskets

Made from stainless steel and has a positive clip-on cover. It is ideal for processing micro specimens. It can be used as a holder for paraffin sections while removing paraffin wax. Available in medium and fine mesh. It measures: 20mm(dia.)x 5mm(deep).

62312-10 Micro Baskets, Medium Mesh each 22.50 Add to Cart
62312-16 Micro Baskets, Medium Mesh 6/pk 112.00 Add to Cart
62312-20 Micro Baskets, Fine Mesh each 22.00 Add to Cart
62312-26 Micro Baskets, Fine Mesh 6/pk 112.00 Add to Cart

Expandable Capsules

Made from stainless steel and comes with a positive snap cap. They are expandable. When closed its dimensions are 40mm(dia.)x 7mm (deep) and can be expanded to 11mm deep. Perforated top and bottom.

62314-01 Expandable Capsule each 29.00 Add to Cart
62314-06 Expandable Capsule 6/pk 160.00 Add to Cart

Multi-Purpose Capsules

Product 62316 no longer is available. Please see 62320.

Stainless Steel Tissue Capsules

Features:

  • A secure positive snap-on cover.
  • Perforated on the top and bottom for easy fluid circulation.
  • Can be partitioned for up to 4 sections.

The separator is not included
They measure: 37mm(I.D.) x 6mm(deep) (17/16″ x 3/16″)

62320-01 SS Tissue Capsule each 18.00 Add to Cart
62320-06 SS Tissue Capsule 6/pk 82.00 Add to Cart
62320-20 Tissue Capsule Separator 2/pk 19.00 Add to Cart

Plastic Tissue Capsules

Features:

  • Molded from polyethylene; resistant to all processing chemicals, including Zenker’s fixative.
  • A three-compartment divider which is removable for specimens up to 35mm.
  • A weighted end floats the capsule in a upright position to conserve space.
  • Perforated on the top and bottom for better circulation of the fluid.
  • Snap-on hinged cover.

They measure: 35mm(I.D.)x35mm(I.D.)x10mm(deep) (13/8×5/16)

62322-12 Plastic Tissue Capsules 12/pk 70.00 Add to Cart

Specimen Discs

Accuray machined chrome plated aluminum discs that fit most microtomes and cryostats. They have a knurled surface for better specimen handling. Straight shank. Available in a variety of disc sizes.

62355-01 Specimen Disc, size 7/8″ (2.2cm) each 53.00 Add to Cart
62355-04 Specimen Disc, size 17/8″ (2.9cm) each 54.00 Add to Cart
62355-07 Specimen Disc, size 1½” (3.8cm) each 62.00 Add to Cart
62355-10 Specimen Disc, size 2″ (5.1cm) each 58.00 Add to Cart

Tissue Processing Capsules

These one piece plastic capsules are hinged and perfectly aligned, offering convenience and ease of use. Both the top and the bottom of the capsule have an open mesh offering maximum fluid flow during processing. A frosted marking surface assures permanent identification. These capsules are commonly used with the Embedding Ring System. They are available in two sizes and five colors.

To Use: Simply place the tissue in the capsule, close the cover to lock, and the sample is now ready for processing.

  • The 38mm(Dia.)x8mm(Deep) are intended for use with normal tissue.
  • The 29mm(Dia.)x6mm(Deep) are intended for use with Biopsy tissue
Cat. No Color Capsule Size Pack Price  
62358-W White 38mmx8mm 500 105.00 Add to Cart
62359-W White 29mmx6mm 500 105.00 Add to Cart

Embryo Dishes

These dishes are molded from clear glass and come with a glass lid for the viewing or staining of free-floating specimens.

70543-30 Embryo Dish, 30 mm Dia x 12mm Deep each 8.50 Add to Cart
70543-45 Embryo Dish, 45 mm Dia x 12mm Deep each 14.50 Add to Cart

Paper Biopsy Bags

Due to the amazing demand from of our customers, EMS is re-introducing the original Paper Biopsy Bags. These bags are made from porous paper which provides maximum tissue safety during processing. The bags permit proper fluid exchange and drainage.

To Use: Simply introduce the specimen into the bag and fold its end, enclosing the cassette or capsule – you are now ready to process as usual.

Bag measurement: Overall 13⁄4” W x 3” L (45 x 75 mm), Inside Seam: 13⁄8” W (35 mm)

62326-01 Biopsy Paper Bags 100/pk 9.00 Add to Cart
62326-05 Biopsy Paper Bags 500/pk 36.00 Add to Cart

Biopsy Foam Pads

A cellular polyester blue foam that has uniform air pockets throughout the pad for optimal fluid flow. It prevents either small or fragmented tissue specimens from being lost.

To Use: Place small samples or specimen fragments between the two pads and place the specimen in a cassette or capsule.

Cat. No. Foam Pad Size Use With Pack Price  
62325-01 25mm Diameter 29mm Capsules 1000/pk 26.00 Add to Cart
62325-04 35mm Diameter 38mm Capsules 1000/pk 30.00 Add to Cart
62325-06 25mmx32mm 1×1¼”Cassettes 1000/pk 23.00 Add to Cart

Biopsy Bags

  • Made from nylon cloth, 0.2mm mesh diameter. All seams are heat-sealed.
  • Allows for unrestricted fluid movement around the tissue.
  • Reduces the loss of small biopsy specimens.
  • Reduces cross contamination and pressure artifacts by using sponges.
62324-35 Nylon Biopsy Bag 35 x 50mm 100/pk 44.00 Add to Cart
62324-75 Nylon Biopsy Bag 45 x 74mm 100/pk 60.00 Add to Cart
62324-98 Nylon Biopsy Bag 98 x 75mm 100/pk 67.00 Add to Cart

CellSafe™ Biopsy Insert

The CellSafe is a disposable plastic biopsy insert consisting of two hinged interlocking frames with an integral nylon mesh. The mesh is extra fine and therefore ensures a high level of specimen security.

Measurements:
Overall: 28 mm x 25 mm x 5 mm high (1.1” x 1” x ¼”);
The mesh area: 18 mm x 18 mm x 2 mm high.

62327-10 CellSafe™ Biopsy Insert 100/pk. 68.00 Add to Cart

Stainless Steel Biopsy Inserts

A two-part loose fitting stainless steel capsule with welded mesh that fits inside the processing cassettes preventing the loss of small or friable material while allowing interchange of fluids.

They fit all process/embedding cassettes such as 70025 and 72025 as well as Uni-Cassettes (62500).

62328-01 Stainless Steel Biopsy Insert each $16.00 Add to Cart

Peel Away Disposable Embedding Molds

For plastic or paraffin embedding; no block trimming needed. Inside fingers hold ID slip which becomes embedded in the block for positive permanent identification.

70180 Truncated Mold, size 8x8mm 288/cs 162.00 Add to Cart
70181 Truncated Mold, size 12x12mm 288/cs 162.00 Add to Cart
70182 Square Mold, size 22x22mm 288/cs 162.00 Add to Cart
70183 Rectangular Mold, size 22x30mm 288/cs 162.00 Add to Cart
70184 Rectangular Mold, size 22x40mm 288/cs 162.00 Add to Cart

Adjustable Embedding Mold

These two “L’s” shape and stainless steel base plate form an adjustable embedding mold, suitable for embedding various size specimens with a minimum of waste material. Available in 5 different sizes.

62361-01 Adjustable Mold, 7⁄8″ x 13⁄4″ x ½” (22x44x13mm) each 58.00 Add to Cart
62361-03 Adjustable Mold, 7⁄8″ x 13⁄4″ x 3⁄4″ (22x44x19mm) each 59.00 Add to Cart
62361-05 Adjustable Mold, 7⁄8″ x 13⁄4″ x 1″ (22x44x25mm) each 59.00 Add to Cart
62361-07 Adjustable Mold, 7⁄8″ x 13⁄4″ x 11⁄4″ (22x44x32mm) each 70.00 Add to Cart
62361-09 Adjustable Mold, 3½” x 53⁄4″ x 1″ (89x146x25mm) each 98.00 Add to Cart

Slide Coating Adhesive

Prepared from 3-aminopropyltriethoxysilane in acetone. Good for coating glass slides to adhere paraffin sections for further processing without sections falling off from the slide. READY TO USE.

71302 Slide Coating Adhesive 100 ml 26.00 Add to Cart

Embedding Blocks

  • Precisely machined from hard phenolic resin .
  • The faces of the embedding blocks are cut very deeply providing a firm hold of the embedded specimen.
  • Suitable for cellodin or paraffin sections.
    Available in four sizes.
62363-01 Embedding Block, 19x19x19mm High each 44.00 Add to Cart
62363-02 Embedding Block, 25x25x19mm High each 50.00 Add to Cart
62363-03 Embedding Block, 38x38x19mm High each 60.00 Add to Cart
62363-04 Embedding Block, 51x51x19mm High each 70.00 Add to Cart

Embedding Molds and Mold Release Agents

For a complete selection, please see Molds and Mold Release Agent

Liquid Blocker–Super PAP Pen

The Newest Staining Guard Pen

New and improved. The Super PAP Pen outperforms the original PAP Pen. The Super PAP Pen may be used at temperatures as high as 120°F. (The old style PAP Pen worked only up to 90°F). With this high temperature feature, one can now use the Super PAP Pen to perform the peroxidase-anti-peroxidase method, ABC method, and Fluorescent antibodies method with satisfactory results.

71310 Super PAP Pen, Large each $58.00 Add to Cart
71312 Super PAP Pen, Small each $40.00 Add to Cart

Fro-Marker

A wide tipped pen designed for use with frozen sections. The pen provides a sticky membrane which when applied to the slide and onto which the section is placed it eliminates the section from falling off, moving, or wrinkling on the slide during immunostaining procedures. The membrane is stable up to 110°C and is suitable for vigorous applications such as in-situ hybridization.

71313-10 Fro-Marker each 85.00 Add to Cart

Para-Marker

A wide tipped pen, designed for use with paraffin sections. The pen provides a sticky membrane to the glass slide which not only prevents paraffin sections from falling off but also keeps the sections flat on the slide.

71313-20 Para-Marker each 74.00 Add to Cart

Tissue Capture Pen

With one swift application of the EMS Tissue Capture Pen, you are able to change your plain glass slide into a coated one.

The EMS Tissue Capture Pen is a coating pen with a wide point (18mm) for fixing various tissue sections (paraffin embedded, frozen and formalin sections, etc.) The unique pen is unaffected by heat up to 120ºC so it offers secure and firm adhesion of the tissue section. It may be used in Microwave staining, immunostaining, in situ etc….

One application is all that is needed, and after it is applied, allow to dry for 1-2 minutes.

The EMS Tissue Capture may be used in conjunction with our Pap-Pen ( 71310) and will allow for easy placement of adherent as well as nonadherent cells on a glass slide. Immunostaining/ISH can be performed by applying a few drops of tissue suspension or liquid media onto the glass slide which is circled by the Pap-Pen, and then your section can be placed.

Ordering

71314-10 EMS Tissue Capture Pen each 92.00 Add to Cart

兔免疫组化(IHC)检测试剂盒 Immunohischemistry Kit for Rabbit Primary Antibody

兔免疫组化(IHC)检测试剂盒 Immunohischemistry Kit for Rabbit Primary Antibody

产品说明书

FAQ

COA

已发表文献

产品描述

IHC Kit for Rabbit Primary Antibody可用于检测以兔单克隆或多克隆抗体为一抗的免疫组化实验,检测原理基于高特异性高亲和力的链霉亲和素生物素结合:已固定或培养细胞的抗原与一抗形成抗原抗体复合物,生物素化二抗特异性结合上述复合物,经辣根过氧化物酶标记的链霉亲和素特异性识别生物素(即抗原所在位置),最后经辣根过氧化物酶底物显色即可检测相应抗原。

本品可适用于多种类型样本,如石蜡包埋切片、冰冻切片、培养细胞涂片及血液标本等。

 

产品组分

组分

组分编码

组分名称

产品编号/规格

储存条件

Part Ⅰ

36312-A

1×PBS 缓冲液(干粉,4L

2L×2

室温

36312-B

100×柠檬酸钠抗原修复液

32 mL

28

36312-C

内源性过氧化物酶阻断剂(即用型)

5 mL

28

36312-D

抗体稀释液(即用型)

5 mL

28

36312-E

封闭用正常山羊血清工作液(即用型)

5 mL

28

36312-F

生物素标记羊抗兔 IgG(即用型)

5 mL

28

36312-G

辣根酶标记链酶卵白素工作液(即用型)

5 mL

28

36312-J

苏木素染液(即用型)

6 mL

室温

Part Ⅱ

36312-H

DAB kit (20×)显色液

0.5 mL

20

36312-I

DAB kit (20×)稀释液

0.5 mL

20

 

运输和保存方法

冰袋运输。Part ⅡH,I组分-20℃保存,其他组分4℃保存,有效期1年。勿冰冻!

 

注意事项

1.了您的安全和健康,请穿实验服并戴一次性手套操作。

2.DAB是一潜在致癌物,使用时请注意自我防护。

3.检测样本时需同时做阴性对照和阳性对照。

4.产品仅作科研用途!

 

准备试剂

二甲苯、乙醇、甲醇、去离子水、封片剂(Cat#36313

 

使用方法

1.脱蜡水化:将石蜡切片置于新鲜二甲苯中浸泡15 min,重复三次。去除多余的液体后,依次置于无水乙醇、95%乙醇、90%乙醇、80%乙醇、70%乙醇,各浸泡5 min,蒸馏水(或自来水)冲洗5 min。用PBS缓冲液(试剂A,将干粉溶解于2 L去离子水中)冲洗切片5 min,重复三次。

2.抗原修复:将脱蜡水化后的组织切片置于烧杯(或修复盒)中的耐高温塑料切片架上,加适量修复液柠檬酸钠抗原修复液(试剂B,将100×柠檬酸钠抗原修复液加去离子水稀释成柠檬酸钠抗原修复液),液面要浸过切片组织一定高度,将修复盒置于沸水中煮沸修复15 min后取出玻片,自然凉至室温。用PBS缓冲液冲洗切片5 min,重复三次。注意:抗原修复时,修复液务必保证切片始终浸泡在液体内,一般情况:修复液的量约为800 mL/1架。

3.阻断内源性过氧化物酶:用吸水纸擦干玻片,免疫组化笔在组织周围画圈,滴加50 μL内源性过氧化物酶阻断剂(试剂C到切片组织上以阻断内源性过氧化物酶,室温孵育30 min,用PBS缓冲液冲洗切片5 min,重复三次。

4.血清封闭:用吸水纸擦干玻片,滴加50 μL正常山羊血清工作液(试剂E),37℃封闭20 min,以减少非特异性染色。

5.一抗孵育:用抗体稀释液(试剂 D)将一抗原液稀释成工作液,用吸水纸擦干玻片组织周围的液体,滴加适量抗体工作液,置于孵育4℃孵育过夜或37℃孵育1h2h

6.复温:4℃过夜后从冰箱拿出样本,在室温下孵育15 min复温(抗体孵育为4℃过夜选用此步骤,如室温孵育直接进入下一步清洗)。用PBS缓冲液冲洗切片5 min,重复三次。

7.二抗孵育:吸水纸擦干切片后滴加50 μL生物素标记的羊抗兔IgG(试剂F37℃孵育20 min,用PBS缓冲液冲洗切片5 min,重复三次。

8.三抗孵育:吸水纸擦干切片后滴加50 μL 辣根酶标记的链酶卵白素孵育(试剂G37℃孵育20 min,用PBS缓冲液冲洗切片5 min,重复三次。

9.显色:甩去 PBS 缓冲液,吸水纸擦干切片;每张切片滴加新鲜配制的DAB工作液50 μL(试剂H:试剂I:试剂A=1:1:18比例配置),孵育3-5 min,光学显微镜下观察染色结果,切勿显色过深;显色后用自来水冲洗切片终止显色。

【注】:1. DAB显色液需现配现用,避光放置,且在30 min内使用。

2.可根据需要一次染多张切片,各种试剂量按照上述比例放大即可。

10.复染:滴加适量苏木素染液(试剂J)复染,孵育1-5 min,自来水冲洗5 min,滴加盐酸酒精分化液分化30 sec,自来水冲洗。

11.脱水封片:将玻片依次置于70%乙醇、80%乙醇、90%乙醇、95%乙醇、无水乙醇,各浸泡 5 min,再将玻片放入二甲苯

中浸泡15 min,重复三次。玻片取出来稍晾干,用中性树胶封片。

12.结果判定:

在光学显微镜下对染色的切片观察并判断。苏木素染细胞核为蓝色,DAB显色的阳性表达为棕黄色。

 

HB221025

Q:该产品适用于石蜡包埋切片、冰冻切片、培养细胞涂片及血液标本吗?

A:适用。

Q:该产品需在 4℃下储存吗?

A:部分Ⅱ,H,I 组分-20℃保存,其他组分 4℃保存,勿冰冻。

Q:配置好的 DAB 显色液可以保存多久?

ADAB 显色液需现配现用,避光放置,且在 30 min 内使用。

Q:该产品可一次染多张切片吗?

A:可根据需要一次染多张切片,各种试剂量按照说明书比例放大即可。

[1] Li M, Li SC, Dou BK, et al. Cycloastragenol upregulates SIRT1 expression, attenuates apoptosis and suppresses neuroinflammation after brain ischemia. Acta Pharmacol Sin. 2020;41(8):1025-1032. doi:10.1038/s41401-020-0386-6(IF:5.064)

产品描述

IHC Kit for Rabbit Primary Antibody可用于检测以兔单克隆或多克隆抗体为一抗的免疫组化实验,检测原理基于高特异性高亲和力的链霉亲和素生物素结合:已固定或培养细胞的抗原与一抗形成抗原抗体复合物,生物素化二抗特异性结合上述复合物,经辣根过氧化物酶标记的链霉亲和素特异性识别生物素(即抗原所在位置),最后经辣根过氧化物酶底物显色即可检测相应抗原。

本品可适用于多种类型样本,如石蜡包埋切片、冰冻切片、培养细胞涂片及血液标本等。

 

产品组分

组分

组分编码

组分名称

产品编号/规格

储存条件

Part Ⅰ

36312-A

1×PBS 缓冲液(干粉,4L

2L×2

室温

36312-B

100×柠檬酸钠抗原修复液

32 mL

28

36312-C

内源性过氧化物酶阻断剂(即用型)

5 mL

28

36312-D

抗体稀释液(即用型)

5 mL

28

36312-E

封闭用正常山羊血清工作液(即用型)

5 mL

28

36312-F

生物素标记羊抗兔 IgG(即用型)

5 mL

28

36312-G

辣根酶标记链酶卵白素工作液(即用型)

5 mL

28

36312-J

苏木素染液(即用型)

6 mL

室温

Part Ⅱ

36312-H

DAB kit (20×)显色液

0.5 mL

20

36312-I

DAB kit (20×)稀释液

0.5 mL

20

 

运输和保存方法

冰袋运输。Part ⅡH,I组分-20℃保存,其他组分4℃保存,有效期1年。勿冰冻!

 

注意事项

1.了您的安全和健康,请穿实验服并戴一次性手套操作。

2.DAB是一潜在致癌物,使用时请注意自我防护。

3.检测样本时需同时做阴性对照和阳性对照。

4.产品仅作科研用途!

 

准备试剂

二甲苯、乙醇、甲醇、去离子水、封片剂(Cat#36313

 

使用方法

1.脱蜡水化:将石蜡切片置于新鲜二甲苯中浸泡15 min,重复三次。去除多余的液体后,依次置于无水乙醇、95%乙醇、90%乙醇、80%乙醇、70%乙醇,各浸泡5 min,蒸馏水(或自来水)冲洗5 min。用PBS缓冲液(试剂A,将干粉溶解于2 L去离子水中)冲洗切片5 min,重复三次。

2.抗原修复:将脱蜡水化后的组织切片置于烧杯(或修复盒)中的耐高温塑料切片架上,加适量修复液柠檬酸钠抗原修复液(试剂B,将100×柠檬酸钠抗原修复液加去离子水稀释成柠檬酸钠抗原修复液),液面要浸过切片组织一定高度,将修复盒置于沸水中煮沸修复15 min后取出玻片,自然凉至室温。用PBS缓冲液冲洗切片5 min,重复三次。注意:抗原修复时,修复液务必保证切片始终浸泡在液体内,一般情况:修复液的量约为800 mL/1架。

3.阻断内源性过氧化物酶:用吸水纸擦干玻片,免疫组化笔在组织周围画圈,滴加50 μL内源性过氧化物酶阻断剂(试剂C到切片组织上以阻断内源性过氧化物酶,室温孵育30 min,用PBS缓冲液冲洗切片5 min,重复三次。

4.血清封闭:用吸水纸擦干玻片,滴加50 μL正常山羊血清工作液(试剂E),37℃封闭20 min,以减少非特异性染色。

5.一抗孵育:用抗体稀释液(试剂 D)将一抗原液稀释成工作液,用吸水纸擦干玻片组织周围的液体,滴加适量抗体工作液,置于孵育4℃孵育过夜或37℃孵育1h2h

6.复温:4℃过夜后从冰箱拿出样本,在室温下孵育15 min复温(抗体孵育为4℃过夜选用此步骤,如室温孵育直接进入下一步清洗)。用PBS缓冲液冲洗切片5 min,重复三次。

7.二抗孵育:吸水纸擦干切片后滴加50 μL生物素标记的羊抗兔IgG(试剂F37℃孵育20 min,用PBS缓冲液冲洗切片5 min,重复三次。

8.三抗孵育:吸水纸擦干切片后滴加50 μL 辣根酶标记的链酶卵白素孵育(试剂G37℃孵育20 min,用PBS缓冲液冲洗切片5 min,重复三次。

9.显色:甩去 PBS 缓冲液,吸水纸擦干切片;每张切片滴加新鲜配制的DAB工作液50 μL(试剂H:试剂I:试剂A=1:1:18比例配置),孵育3-5 min,光学显微镜下观察染色结果,切勿显色过深;显色后用自来水冲洗切片终止显色。

【注】:1. DAB显色液需现配现用,避光放置,且在30 min内使用。

2.可根据需要一次染多张切片,各种试剂量按照上述比例放大即可。

10.复染:滴加适量苏木素染液(试剂J)复染,孵育1-5 min,自来水冲洗5 min,滴加盐酸酒精分化液分化30 sec,自来水冲洗。

11.脱水封片:将玻片依次置于70%乙醇、80%乙醇、90%乙醇、95%乙醇、无水乙醇,各浸泡 5 min,再将玻片放入二甲苯

中浸泡15 min,重复三次。玻片取出来稍晾干,用中性树胶封片。

12.结果判定:

在光学显微镜下对染色的切片观察并判断。苏木素染细胞核为蓝色,DAB显色的阳性表达为棕黄色。

 

HB221025

Q:该产品适用于石蜡包埋切片、冰冻切片、培养细胞涂片及血液标本吗?

A:适用。

Q:该产品需在 4℃下储存吗?

A:部分Ⅱ,H,I 组分-20℃保存,其他组分 4℃保存,勿冰冻。

Q:配置好的 DAB 显色液可以保存多久?

ADAB 显色液需现配现用,避光放置,且在 30 min 内使用。

Q:该产品可一次染多张切片吗?

A:可根据需要一次染多张切片,各种试剂量按照说明书比例放大即可。

[1] Li M, Li SC, Dou BK, et al. Cycloastragenol upregulates SIRT1 expression, attenuates apoptosis and suppresses neuroinflammation after brain ischemia. Acta Pharmacol Sin. 2020;41(8):1025-1032. doi:10.1038/s41401-020-0386-6(IF:5.064)