假尿苷三磷酸钠盐溶液(单组分pUTP)
产品说明书
FAQ
COA
已发表文献
Pseudouridine-5-triphosphate, trisodium salt solution is one of the most commonly used modified nucleoside triphosphates.. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics, and reduces its immunogenicity. It has a wide range of applications in the field of therapy and diagnosis.
This product is produced in accordance with GMP process requirements and provided in liquid form.
Product Properties
|
CAS No. |
1175-34-4 (free acid) |
|
Molecular formula |
C9H12N2Na3O15P3 |
|
Molecular weight |
550.10 g/moL |
|
Structure |
|
|
Purity |
≥ 99% |
|
Content |
100 mM ± 3 mM |
Shipping and Storage
The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.
Cautions
- It can be dissolved at room temperature. After dissolution, it should be stored in an ice box or on an ice bath. After use, it should be stored at -20°C immediately.
- For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
HB220902
Pseudouridine-5-triphosphate, trisodium salt solution is one of the most commonly used modified nucleoside triphosphates.. It is mostly used as the reaction substrate or coenzyme of enzymes, such as in vitro transcription, RNA amplification, siRNA synthesis, etc. The modified mRNA containing pseudouridine has better nuclease stability and translation characteristics, and reduces its immunogenicity. It has a wide range of applications in the field of therapy and diagnosis.
This product is produced in accordance with GMP process requirements and provided in liquid form.
Product Properties
|
CAS No. |
1175-34-4 (free acid) |
|
Molecular formula |
C9H12N2Na3O15P3 |
|
Molecular weight |
550.10 g/moL |
|
Structure |
|
|
Purity |
≥ 99% |
|
Content |
100 mM ± 3 mM |
Shipping and Storage
The products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.
Cautions
- It can be dissolved at room temperature. After dissolution, it should be stored in an ice box or on an ice bath. After use, it should be stored at -20°C immediately.
- For your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.
HB220902
Q: UTP的消光系数为10,000 Lmol-1cm-1 at 262 nm，而N1-甲基假UTP的消光系数为8,877 Lmol-1cm-1 at 271 nm，二者差别较大，在用N1-甲基假UTP替代UTP转录的mRNA A260/A280小于2.0，原有的mRNA浓度计算公式RNA浓度(ng/uL) =0D260xN(稀释倍数)x40就不在适用了，请问浓度计算公式系数40变为多少合适？有相关研究数据或文献资料分享吗？
A: 目前没有了解过是否影响计算，NanoDrop 的检测方法比较粗略，如果想要更精确的检测，可以使用Qubit检测RNA的浓度，这两种方法也是市面上比较常见的两种检测方法。
Q: 由于之前使用的其他公司的UTP，想要用翌圣的原料去替换，是否使用方法不同？
A: 一般采用完全替代的方式，假尿苷或者 N1-甲基假尿苷的使用量与原体系尿苷的使用量一致。
暂无内容
