标签归档：产品代理

Quansys Biosciences 产品代理

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Quansys Biosciences 产品代理

简要描述：Quansys公司的Q-View Imager细胞因子阵列分析仪，支持基于96孔板的化学发光成像和化学发光Western blot成像。应用领域是蛋白质和核酸研究，具体包括Western分析，In-Gel Western分析，蛋白质定量分析，双色磷酸化分析，考马斯亮兰胶的扫描，蛋白双向电泳，双色EMSA，双色微孔板分析，Southern Blot等。Quansys Biosciences

详细介绍

产品咨询

世界*实验材料供应商Quansys Biosciences上海金畔生物为其中国代理，Quansys Biosciences 在一直是行业的*,一直为广大科研客户提供zui为优质的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， Quansys Biosciences 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Quansys Biosciences 中国代理，Quansys Biosciences 上海代理，Quansys Biosciences北京代理，Quansys Biosciences 广东代理，Quansys Biosciences 江苏代理 Quansys Biosciences 湖北代理, Quansys Biosciences 天津, Quansys Biosciences 黑龙江代理, Quansys Biosciences内蒙古代理, Quansys Biosciences 吉林代理, Quansys Biosciences 福建代理, Quansys Biosciences 江苏代理, Quansys Biosciences 浙江代理, Quansys Biosciences 四川代理,

Quansys Biosciences公司的主打产品Q-Plex?芯片基于多重ELISA检测技术，通过含有2400种捕获抗体的抗体芯片实现对免疫原的高通量检测，研究人员可分析大鼠（小鼠或人）细胞因子、化学增活素、免疫球蛋白、脂肪因子、血管生成、血细胞生成、肿瘤和激素的标志物等多种免疫原。      

  Quansys Biosciences公司的Q-View Imager细胞因子阵列分析仪，支持基于96孔板的化学发光成像和化学发光Western blot成像。应用领域是蛋白质和核酸研究，具体包括Western分析，In-Gel Western分析，蛋白质定量分析，双色磷酸化分析，考马斯亮兰胶的扫描，蛋白双向电泳，双色EMSA，双色微孔板分析，Northern Blot，Southern Blot等；可以满足生命科学研究、蛋白质组学、基因组学、药物开发和高通量筛选技术（HTS）各领域内的科研需求。另外，还包含软件包Q-View Software，可直接进行Q-Plex阵列和其他化学发光检测数据分析。

我们公司zui大优势是强大的采购，

1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，

2： 货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，

3：提供加急服务，一般1-2周到货，超过时限加急费全免

4：价格公道，绝大部分价格有优势，当然不能保证100%产品都是,因为意味着没有服务.

5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大 交大 复旦，中山等100多所大学，ROCHE，阿斯利康，国药 ，fisher等500多家公司

6：我们还是santa,Quansys Biosciences Inc;Athens Research & Technology, bangs, BBInternational, crystalchem, dianova, Quansys Biosciences, Inc.  Genebridege; Quansys Biosciences Biotechnology GmbH; iduron; Quansys Biosciences； Quansys Biosciences； neuroprobe; omicronbio; Polysciences; prospecbi; QA-BIO；quickzyme；RESEARCH DIETS, INC；sterlitech；sysy；TriLink BioTechnologies, Inc；worthington-biochem;zyagen;……几十家国外公司代理

7：我们还从事invitrogen，qiagen; abcam ;sigma;neb; roche;merck; rnd; BD; GE; pierce; Quansys Biosciences….等*批发

上海金畔生物科技有限公司

琼脂糖凝胶产品代理

发表于由

 

产  品

分离范围/

载量

粒径µm

应  用

PH

流速（cm/h）

琼脂糖凝胶 2B

70,000-40 ×106

60-200

蛋白、大分子复合物、病毒、核酸、多糖的分离、分子量测定

4-9

110

琼脂糖凝胶 4B

60,000-20 ×106

45-165

蛋白、多肽、多糖的分离、分子量测定

4-9

115

琼脂糖凝胶 6B

10,000-4 ×106

45-165

蛋白、多肽、多糖的分离、分子量测定

4-9

140

琼脂糖凝胶CL-2B

70,000-40 ×106

60-200

蛋白、大分子复合物、病毒、核酸、多糖的分离、分子量测定

3-14

150

琼脂糖凝胶CL-4B

60,000-20 ×106

45-165

蛋白、多肽、多糖的分离、分子量测定，特别适合不溶于水的大分子

3-14

260

琼脂糖凝胶CL-6B

10,000-4 ×106

45-165

蛋白、多肽、多糖的分离、分子量测定，特别适合不溶于水的大分子

3-14

300

琼脂糖凝胶 4FF

60,000-20 ×106

45-165

巨大分子病毒、疫苗的分离

2-14

250

琼脂糖凝胶 6FF

10,000-4 ×106

45-165

巨大分子DNA质粒、病毒  的分离

2-14

300

SP-琼脂糖凝胶 H.P.

55mg RNase A

10-45

医药工业精细分离纯化

4-13

150

SP –琼脂糖凝胶FF

70mg RNase A

45-165

医药工业快速高产量纯化

3-14

750

CM-琼脂糖凝胶 FF

50mg RNase A

45-165

医药工业快速高产量纯化

2-14

750

CM-琼脂糖凝胶CL-6B

120mg RNase A

45-165

医药工业精细分离纯化

3-12

150

Q-琼脂糖凝胶 H.P.       

70mg HSA

10-45

医药工业精细分离纯化

2-12

150

Q –琼脂糖凝胶 FF

120mg HSA

45-165

医药工业快速高产量纯化

2-12

750

DEAE-琼脂糖凝胶FF

110mg HSA

45-165

医药工业快速高产量纯化

2-13

750

DEAE-琼脂糖凝胶CL-6B

170mg HSA

45-165

医药工业精细分离纯化

3-12

150

苯基–琼脂糖凝胶 6FF

40 µmol

45-165

医药工业精细分离纯化

2-14

450

苯基–琼脂糖凝胶H.P

25 µmol

10-45

医药工业精细分离纯化

2-14

100

苯基–琼脂糖凝胶CL-4B

40 µmol

10-45

医药工业精细分离纯化

2-14

150

丁基–琼脂糖凝胶 4FF

40 µmol

45-165

医药工业精细分离纯化

3-12.5

240

丁基–琼脂糖凝胶H.P

50 µmol

10-45

医药工业精细分离纯化

3-12.5

100

丁基–琼脂糖凝胶4B

10-14 µmol

45-165

医药工业精细分离纯化

3-12.5

150

辛基–琼脂糖凝胶 4FF

5 µmol

45-165

医药工业精细分离纯化

3-12

240

辛基–琼脂糖凝胶H.P

25 µmol

10-45

医药工业精细分离纯化

3-12

100

辛基–琼脂糖凝胶CL-4B

40 µmol

45-165

医药工业精细分离纯化

3-12

150

镍–琼脂糖凝胶 6FF

10-20 µmol

45-165

分离含有多个组氨酸的蛋白以及对金属离子有吸附作用的多肽、蛋白和核苷酸

3-10

150

镍–琼脂糖凝胶 H.P.

10-20 µmol

10-45

分离含有多个组氨酸的蛋白以及对金属离子有吸附作用的多肽、蛋白和核苷酸

3-10

150

Blue-琼脂糖凝胶 6FF

7 µmol

45-165

适用于白蛋白、干扰素、α2巨球蛋白、凝血因子及各种需要NAD+和NADP+的酶的分离纯化

3-10

300

Blue-琼脂糖凝胶 CL-6B

2 µmol

45-165

适用于白蛋白、干扰素、α2巨球蛋白、凝血因子及各种需要NAD+和NADP+的酶的分离纯化

3-10

150

肝素–琼脂糖凝胶 6FF

4mg/ml

45-165

用于抗凝血因子Ⅲ、凝血因子、蛋白合成因子、脂蛋白、干扰素、核酸结合蛋白、限制内切酶、凝血酶及类凝血酶等生物大分子的分离纯化

4-10

300

肝素–琼脂糖凝胶 H.P.

4mg/ml

10-45

用于抗凝血因子Ⅲ、凝血因子、蛋白合成因子、脂蛋白、干扰素、核酸结合蛋白、限制内切酶、凝血酶及类凝血酶等生物大分子的分离纯化

4-10

150

谷胱甘肽–琼脂糖凝胶4B

5mg/ml

45-165

用于纯化含有GST标签的重组蛋白

4-13

75

谷胱甘肽–琼脂糖凝胶4FF

5mg/ml

45-165

用于纯化含有GST标签的重组蛋白

3-12

100

谷胱甘肽–琼脂糖凝胶HP

5mg/ml

10-45

用于纯化含有GST标签的重组蛋白

3-12

300

胰蛋白酶–琼脂糖凝胶4B

≤10mg/ml

45-165

用于纯化一些能和它结合的物质，如丝氨酸蛋白酶抑制剂等

1.5-10

150

苯甲醚–琼脂糖凝胶 6B

7 µmol/ml

45-165

用于胰蛋白酶、凝血酶、尿激酶、激肽释放酶、前激肽释放酶等丝氨酸蛋白酶的分离纯化

3-10

75

苯甲醚–琼脂糖凝胶 4FF

14 µmol/ml

45-165

用于胰蛋白酶、凝血酶、尿激酶、激肽释放酶、前激肽释放酶等丝氨酸蛋白酶的分离纯化

2-9

300

ConA-琼脂糖凝胶 4B

10-16 mg/ml

45-165

用于各类带甘露醇及葡萄糖残基的糖、糖蛋白、糖脂的分离纯化

4-9

100

赖氨酸–琼脂糖凝胶 4B

4-7 µmol/ml

45-165

用于纯化纤维蛋白溶酶原激活剂及核糖核糖蛋白体核酸

2-11

75

羧基–琼脂糖凝胶 4B

12-16µmol/ml

45-165

适用于固定化小分子

3-14

75

明胶–琼脂糖凝胶 4FF

5mg/ml

45-165

用于纯化或除去纤维连接蛋白

3-10

200

Phosphosolutions品牌产品代理

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Phosphosolutions品牌产品代理

简要描述：Phosphosolutions公司

详细介绍

产品咨询

 公司概况

 

背景

基因工程– Phosphosolutions是*代可以完整描绘人体的遗传物质序列的企业。

蛋白质体学项目：Phosphosolutions是第二代试图将所有体内蛋白质表达出来的企业。

PhosphoSolutions公司—第三步我们将超越蛋白质体学 进而 专注于磷蛋白质。

 

our focus 专业特色

PhosphoSolutions公司专注于蛋白质组学中的一个（10-20%）含量的小部分磷蛋白质。磷蛋白是监管控制组蛋白质的关键,这一部分是被称为phosphosome蛋白质。磷蛋白被认为是在神经系统疾病如老年痴呆症和癌症方面的关键元素，实质上,phosphosome是蛋白质组学作物的精华。

 

公司目标

简明概述：我们要成为世界上的磷蛋白组的提供者。

方案#1， 特异性磷抗体：首先我们要准备磷蛋白组。在激活或磷酸化状态下磷蛋白组是蛋白质识别研究中的*关键工具。

 

Antibodies 抗体

特异性磷抗体：Detection and quantitation of changes in the state of phosphorylation of specific proteins is of great utility in the quest to establish the function of a given protein and the consequences of its reversible phosphorylation. Two methods commonly used to measure protein phosphorylation and dephosphorylation in cell preparations employ prelabeling with 32Pi or back phosphorylation. These methods continue to be very effective and have advantages for many test systems, but they do have several practical and theoretical limitations （Nestler and Greengard, 1984）. Based in large part on the successful use of short synthetic peptides to produce epitope-targeted antibodies （Lerner, 1982;Sutcliffe et al., 1983）, an immunochemical approach became an attractive alternative for detecting changes in the state of phosphorylation of specific proteins at a specific site. The use of phosphorylation state-specific antibodies takes advantage of the sensitivity and selectivity afforded by immunochemical methodology, combined with relatively simple preparation and potentially broad applications.

The first report of phosphorylation-dependent antibodies appeared in 1981, when polyclonal antibodies that could detect phosphotyrosine-containing proteins were produced by immunization with benzyl phosphonate conjugated to keyhole limpet hemocyanin （KLH） （Ross et al., 1981）. Shortly thereafter, Nairn and colleagues reported the production of serum antibodies that distinguished between the phospho- and dephospho-forms of G-substrate, a protein localized to cerebellar Purkinje cells and phosphorylated by cGMP-dependent protein kinase （Nairn et al., 1982）. A synthetic heptapeptide, Arg-Lys-Asp-Thr-Pro-Ala-Leu, corresponding to a repeated sequence surrounding two phosphorylated threonyl residues in the intact protein, served as antigen. Rabbit antisera against a peptide-KLH conjugate were specific for the dephospho-form of G-substrate. Phospho-specific antibodies were prepared by immunization of rabbits with the purified phosphoprotein, phosphorylated in vitro to a stoichiometry of 2 mol/mol with cGMP-dependent protein kinase. Despite this initial success, other attempts in our laboratory to produce phospho-specific polyclonal antisera by immunization with the phospho-form of intact proteins were not very successful, probably because of two significant factors. First, many phosphorylated proteins are believed to undergo rapid dephosphorylation during immunization, regardless of the route of injection, leading to the loss of the desired phospho-epitope. Second, holoproteins generally contain multiple immunogenic epitopes; this decreases the probability that colonal dominance for a phospho-specific epitope will be obtained.

Taking a more direct approach utilizing phosphorylated and unphosphorylated forms of synthetic phosphopeptides, we developed a general protocol for the production of phosphorylation state-specific antibodies for substrates with established site（s） of phosphorylation （Czernik et al., 1991））. In early stages of our development of this methodology, phosphopeptides were routinely prepared by enzymatic phosphorylation （Czernik et al., 1991）. Although this approach remains perfectly valid today, the preparation of synthetic phosphopeptides using Fmoc derivatives of phosphoamino acids has become the state-of-the-art （Czernik et al., 1995;Czernik et al., 1996）. Likewise, we have examined the use of both polyclonal and monoclonal techniques for antibody production. Given the high success rate that we and others have obtained with the polyclonal technique, it has become the method of choice, because it is an easier and less costly method for the average laboratory. However, when appropriate, this approach can be readily adapted for monoclonal antibody production.

参考文献

1. Czernik AJ, Girault J-A, Nairn AC, Chen J, Snyder G, Kebabian J, Greengard P （1991） Production of phosphorylation state-specific antibodies. Methods Enzymol 201: 264-283.

2. Czernik AJ, Mathers J, Mische SM （1997） Phosphorylation state-specific antibodies. Neuromethods: Regulatory Protein Modification: Techniques & Protocols 30: 219-250.

3. Czernik AJ, Mathers J, Tsou K, Greengard P, Mische SM （1995） Phosphorylation state-specific antibodies: preparation and applications. Neuroprotocols 6: 56-61.

4. Lerner, R. A. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299, 593-596. 1982.

5. Nairn AC, Detre JA, Casnellie JE, Greengard P （1982） Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein. Nature （Lond ） 299: 734-736.

6. Nestler, E. J. and Greengard, P. Protein Phosphorylation in the Nervous System. Nestler and Greengard. Protein Phosphorylation in the Nervous System. [8], 255-299. 1984. New York, Wiley. 

8. Sutcliffe JG, Shinnick TM, Green N, Lerner RA （1983） Antibodies that react with predetermined sites on proteins. Science 219: 660-666.

主营产品清单如下：

 

Anti-Alpha II Spectrin-NEW!Datasheet: Cat #: 99-A2SR

Formulation: polyclonal antibody

Specificity: Rat, Mouse, Human

Species: Rabbit     Applications: WB, IF$275.00

for 100 ul

QTYORDER

add to cart

Anti-Alpha Internexin （NF66）Datasheet: Cat #: 101-AIN

Formulation: Total IgY fraction

Specificity: Rat, Mouse, Human

Species: Chicken     Applications: WB, IF$275.00

for 100 µl

QTYORDER

add to cart

Anti-Alpha Internexin （NF66）Datasheet: Cat #: 100-AIN

Formulation: Total IgG fraction

Specificity: Most mammals

Species: Mouse     Applications: WB, IF, IHC$275.00

for 100 µl

QTYORDER

add to cart

Anti-alpha SynucleinDatasheet: Cat #: 107-ASYN

Formulation: mouse ascites fluid

Specificity: Rat, Mouse, Human

Species: Mouse     Applications: WB, IF$275.00

for 100 ul

QTYORDER

add to cart

Anti-Alpha Synuclein （Ser129）Datasheet: Cat #: p1571-129

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Non-human primate, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-Amyloid Precursor ProteinDatasheet: Cat #: 144-APP

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Non-human primate, Canine

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-Aquaporin2 （Ser261）Datasheet: Cat #: p112-261

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-Aquaporin2 （Ser264）Datasheet: Cat #: p112-264

Formulation: Affinity purified polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-Aquaporin2 （Ser269）Datasheet: Cat #: p112-269

Formulation: Affinity purified polyclonal antibody

Specificity: Rat, Mouse, Bovine, Canine

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-ATF2 （Ser490,498）Datasheet: Cat #: p115-4908

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Human

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-ATF2 （Thr52）-NEW!Datasheet: Cat #: p115-52

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-Beclin-1 （Ser234）-NEW!Datasheet: Cat #: p117-234

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Non-human primate, Sheep, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-Beclin-1 （Ser295）-NEW!Datasheet: Cat #: p117-295

Formulation: Affinity purified, polyclonal antibody

Specificity: Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Sheep, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-Calcitonin Gene Related Peptide （CGRP）Datasheet: Cat #: 311-CGRP

Formulation: affinity purified monoclonal antibody

Specificity: Rat, Human, Most mammals

Species: Mouse     Applications: ICC$325.00

for 100 ul

QTYORDER

add to cart

Anti-CalretininDatasheet: Cat #: 303-CAL

Formulation: Affinity purified polyclonal antibody

Specificity: Rat, Mouse, Human, Non-human primate

Species: Sheep     Applications: WB, IF, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-CaM Kinase II （Thr286）Datasheet: Cat #: p1005-286

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-CaM Kinase II （Thr305）Datasheet: Cat #: p1005-305

Formulation: Serum

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Zebra fish, Canine

Species: Rabbit     Applications: WB$275.00

for 100 µl

QTYORDER

add to cart

Anti-Catenin, β- （Ser33,37）Datasheet: Cat #: p120-3337

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-cdc2 （Tyr15）Datasheet: Cat #: p135-15

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Zebra fish

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-CDK5Datasheet: Cat #: 308-CDK5

Formulation: Purified mouse monoclonal antibody

Specificity: Rat, Mouse, Human

Species: Mouse     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-Choline AcetyltransferaseDatasheet: Cat #: 316-ChAT

Formulation: neat serum

Specificity: Rat, Mouse, Human, Chicken, Non-human primate, Guinea Pig

Species: Rabbit     Applications: WB, IHC$275.00

for 100 µl

QTYORDER

add to cart

Anti-Choline AcetyltransferaseDatasheet: Cat #: 315-ChAT

Formulation: Affiinity Purified, goat polyclonal antibody

Specificity: Rat, Mouse, Human, Chicken, Non-human primate, Guinea Pig

Species: Goat     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-Clavesin1/2Datasheet: Cat #: 317-CLAV

Formulation: Affinity purified polyclonal antibody

Specificity: Rat, Mouse, Human

Species: Rabbit     Applications: WB, ICC$325.00

for 100 ul

QTYORDER

add to cart

Anti-CNP （CNPase）Datasheet: Cat #: 325-CNP

Formulation: neat serum

Specificity: Rat, Mouse, Human, Sheep, Rabbit

Species: Rabbit     Applications: WB, IHC$275.00

for 50 ul

QTYORDER

add to cart

Anti-COBRA1 （NELF B）–NEW!Datasheet: Cat #: 319-COBRA

Formulation: Purified mouse monoclonal antibody

Specificity: Rat, Mouse, Human

Species: Mouse     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-Collagen I, alpha 1, propeptideDatasheet: Cat #: 321-COLP

Formulation: Affinity purified polyclonal antibody

Specificity: Rat, Mouse, Human

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-Collagen I, alpha 1, opeptideDatasheet: Cat #: 322-COLT

Formulation: Affinity purified polyclonal antibody

Specificity: Rat, Mouse, Human, Sheep

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-Connexin43Datasheet: Cat #: 327-CON

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Chicken, Non-human primate, Zebra fish, Sheep, Canine

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-Connexin43 （Ser368）Datasheet: Cat #: p1007-368

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Zebra fish, Canine, Guinea Pig

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-COXIV （Ser58）-NEW!Datasheet: Cat #: p1008-58

Formulation: Affinity purified polyclonal antibody

Specificity: Rat, Mouse

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-CREB （Ser133）Datasheet: Cat #: p1010-133

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Sheep, Canine

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-DARPP 32Datasheet: Cat #: 374-DARPP

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Canine

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-DARPP 32 （Ser137）-NEW!Datasheet: Cat #: p1025-137

Formulation: Affinity purified polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-DARPP 32 （Thr34）Datasheet: Cat #: p1025-34

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-DARPP 32 （Thr75）Datasheet: Cat #: p1025-75

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-DGCR8 （DiGeorge Syndrome Critical Region 8） （Ser377）-NEW!Datasheet: Cat #: p1035-377

Formulation: Affinity purified, polyclonal antibody

Specificity: Mouse, Human

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-DOPA DecarboxylaseDatasheet: Cat #: 417-DDC

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Bovine

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-DOPA Decarboxylase, HumanDatasheet: Cat #: 418-DDCH

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Human, Bovine, Sheep, Canine, Rabbit, Guinea Pig

Species: Rabbit     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-Dopamine β-Hydroxylase, C-TerminusDatasheet: Cat #: 420-DBHC

Formulation: Affinity purified, polyclonal antibody

Specificity: Mouse, Human, Non-human primate

Species: Sheep     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-Dopamine β-Hydroxylase, N-TerminusDatasheet: Cat #: 423-DBHN

Formulation: Affinity purified, polyclonal antibody

Specificity: Human, Non-human primate

Species: Sheep     Applications: WB$325.00

for 100 µl

QTYORDER

add to cart

Anti-Dopamine Transporter, C-TerminusDatasheet: Cat #: 431-DATC

Formulation: Affinity purified, polyclonal antibody

Specificity: Mouse, Human, Non-human primate

Species: Rabbit     Applications: WB, IHC$325.00

for 100 µl

QTYORDER

add to cart

Anti-Dopamine Transporter （DAT） （Thr53）-NEW!Datasheet: Cat #: p435-53

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-Dopamine Transporter, Extracellular Loop 2Datasheet: Cat #: 434-DA2

Formulation: Affinity purified, polyclonal antibody

Specificity: Human, Non-human primate

Species: Rabbit     Applications: WB, IHC$325.00

for 100 µl

QTYORDER

add to cart

Anti-Doublecortin （DCX）-NEW!Datasheet: Cat #: 451-DCX

Formulation: Affinity purified mouse monoclonal antibody

Specificity: Rat, Mouse, Human, Bovine

Species: Mouse     Applications: WB, IF$325.00

for 100 ul

QTYORDER

add to cart

Anti-DsbA-L （Disulfide-bond-A oxidoreductase-like protein）Datasheet: Cat #: 465-DsbA-L

Formulation: serum

Specificity: Mouse, Human

Species: Rabbit     Applications: WB, IHC$275.00

for 100 ul

QTYORDER

add to cart

Anti-DynaminDatasheet: Cat #: 480-DYN

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-Dynamin （Ser774）Datasheet: Cat #: p1050-774

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine

Species: Sheep     Applications: WB, IF, IHC, Other$325.00

for 100 ul

QTYORDER

add to cart

Anti-Dynamin （Ser778）Datasheet: Cat #: p1050-778

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Canine

Species: Sheep     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-Elk-1 （Ser383）Datasheet: Cat #: p155-383

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Non-human primate, Zebra fish, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-EphrinB （Tyr298）Datasheet: Cat #: p1110-298

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-EphrinB （Tyr317）Datasheet: Cat #: p1110-317

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Zebra fish

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-EphrinB （Tyr331）Datasheet: Cat #: p1110-331

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Zebra fish

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-ERK/MAPKDatasheet: Cat #: 500-ERK

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-ERK/MAPK （Thr202,Tyr204）Datasheet: Cat #: p160-2024

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Canine

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-FibrillarinDatasheet: Cat #: 560-FIB

Formulation: Total IgG fraction

Specificity: Rat, Human, Drosophila, Some invertebrates

Species: Mouse     Applications: WB, IF$275.00

for 100 ul

QTYORDER

add to cart

Anti-FOX1-NEW!Datasheet: Cat #: 581-FOX1

Formulation: Affinity purified, monoclonal antibody

Specificity: Rat, Mouse, Human, Bovine

Species: Mouse     Applications: WB, IF$325.00

for 100 ul

QTYORDER

add to cart

Anti-FOX1-NEW!Datasheet: Cat #: 581-FOX1

Formulation: Affinity purified, monoclonal antibody

Specificity: Rat, Mouse, Human, Bovine

Species: Mouse     Applications: WB, IF$325.00

for 100 ul

QTYORDER

add to cart

Anti-FOX3 （NeuN）-NEW!Datasheet: Cat #: 583-FOX3

Formulation: Affinity purified, monoclonal antibody

Specificity: Rat, Mouse, Human, Bovine

Species: Mouse     Applications: WB, IF$325.00

for 100 ul

QTYORDER

add to cart

Anti-Fractin （fragment of actin）–NEW!Datasheet: Cat #: 592-FRAC

Formulation: neat serum

Specificity: Rat, Human

Species: Rabbit     Applications: WB, IHC$275.00

for 100 ul

QTYORDER

add to cart

Anti-Fragile X Mental Retardation Protein （FMRP） （Ser499）-NEW!Datasheet: Cat #: p1125-499

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Sheep, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-FtoDatasheet: Cat #: 597-FTO

Formulation: Affinity purified monoclonal antibody

Specificity: Rat, Mouse, Human

Species: Mouse     Applications: WB, IF$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABA B R1 （Ser923）Datasheet: Cat #: p1148-923

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Non-human primate

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABA B R2 （Ser783）Datasheet: Cat #: p1148-783

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate

Species: Rabbit     Applications: WB, IF$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor g2（Ser327）Datasheet: Cat #: p1130-327

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor β1Datasheet: Cat #: 860-GB1C

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor β2Datasheet: Cat #: 862-GB2C

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Non-human primate, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor β3Datasheet: Cat #: 863-GB3C

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor β3（Ser408/409） -NEW!Datasheet: Cat #: p1130-4089

Formulation: Affinity purified polyclonal antibody

Specificity: Rat, Mouse, Human, Xenopus, Bovine, Chicken, Non-human primate, Zebra fish, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor γ2Datasheet: Cat #: 830-GG2

Formulation: Serum

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Zebra fish

Species: Rabbit     Applications: WB, IHC$275.00

for 50 ul

QTYORDER

add to cart

Anti-GABAA Receptor γ2Datasheet: Cat #: 832-GG2C

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Chicken, Non-human primate, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor α1Datasheet: Cat #: 811-GA1C

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Non-human primate, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor α1, N-TerminusDatasheet: Cat #: 812-GA1N

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse, Bovine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor α2Datasheet: Cat #: 840-GA2

Formulation: Serum

Specificity: Rat, Mouse, Human, Bovine, Non-human primate, Canine

Species: Rabbit     Applications: WB$275.00

for 50 ul

QTYORDER

add to cart

Anti-GABAA Receptor α2Datasheet: Cat #: 822-GA2C

Formulation: Affiinity Purified, Polyclonal antibody

Specificity: Rat, Mouse, Human, Bovine, Non-human primate, Zebra fish, Canine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor α3, N-TerminusDatasheet: Cat #: 824-GA3N

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor α4Datasheet: Cat #: 845-GA4C

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Human, Bovine

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor α4, N-TerminusDatasheet: Cat #: 844-GA4N

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat

Species: Rabbit     Applications: WB, IHC$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor α5Datasheet: Cat #: 846-GA5C

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor α6Datasheet: Cat #: 850-GA6

Formulation: Serum

Specificity: Rat, Mouse, Human

Species: Rabbit     Applications: WB$275.00

for 50 ul

QTYORDER

add to cart

Anti-GABAA Receptor α6Datasheet: Cat #: 847-GA6C

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse

Species: Rabbit     Applications: WB$325.00

for 100 ul

QTYORDER

add to cart

Anti-GABAA Receptor, δ-Subunit, N-TerminusDatasheet: Cat #: 868-GDN

Formulation: Affinity purified, polyclonal antibody

Specificity: Rat, Mouse

Species: Rabbit     Applications: WB, IHC, IP$325.00

for 100 ul

Bethyl产品代理

发表于由

Bethyl产品代理

简要描述：Bethyl公司提供各种动物抗体和免疫试剂盒，同时是世界著名的一抗和二抗生产商，成立于1972年，美国农业部（USDA）认证企业，符合GMP标准。

详细介绍

产品咨询

世界*实验材料供应商Bethyl Laboratories上海金畔生物为其中国代理，Bethyl Laboratories在一直是行业的*,一直为广大科研客户提供zui为优质的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， Bethyl Laboratories就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Bethyl Laboratories中国代理，Bethyl Laboratories上海代理，Bethyl Laboratories北京代理，Bethyl Laboratories广东代理，Bethyl Laboratories江苏代理 Bethyl Laboratories湖北代理, Bethyl Laboratories天津, Bethyl Laboratories黑龙江代理, Bethyl Laboratories内蒙古代理, Bethyl Laboratories吉林代理, Bethyl Laboratories福建代理, Bethyl Laboratories江苏代理, Bethyl Laboratories浙江代理, Bethyl Laboratories四川代理,

          Bethyl Laboratories 公司1972 年成立于美国的得克萨斯州，是美国著名的抗体生产商，已有近2,000 种抗体、ELISA 检测试剂盒和RID （放射免疫扩散法）检测试剂盒，应用于细胞生物学和分子生物学研究的各个领域。Bethyl 公司一向秉承“质量至上”的原则，全部产品 均按GMP 标准生产，所有抗体均经抗原亲合层析纯化，以达到zui高的纯度和的亲合力，并且均进行了ELISA，Western Blot、免疫沉淀、免疫组化和免疫细胞化学等检测方法的测试。

Bethyl公司致力于给研究者提供高品质抗体。主要生产抗体为细胞生物学类。公司为临床和科研提供高品质的抗体生产和服务，拥有世界上zui丰富的细胞凋亡产品。如：凯氏半胱氨酸蛋白酶分析试剂盒和试剂，细胞凋亡检测试剂盒及试剂，与细胞凋亡相关的抗体，细胞增殖和细胞毒性检测试剂盒，生长因子，细胞因子和趋化因子，分子生物学试剂盒及试剂等研究产品。

此外，该公司生产的各种动物免疫球蛋白的elisa试剂盒，规格一般为10x96T，性价比优越，颇受广大科研工作者的亲睐。

更多查询请登录Bethyl公司：www.bethyl.com

序列号（Accession Number） 1 NP_065147
基因名（Gene Name） DEAD (Asp-Glu-Ala-Asp) box polypeptide 24
靶标（Target） DDX24
物种（Species） Human
反应活性（Reactivity） Human
结合物/附属物/标记（Conjugate/Tag/Label） Unconjugated
应用（Applications） WB, IP
宿主（Host） Rabbit
类型（Type） Polyclonal
样式（Form） Whole IgG
靶标/分子同义密码子（Target/Molecule Synonym） DDX24, DDX-24, DEAD (Asp-Glu-Ala-Asp) box polypeptide 24.
板式（Format） Whole IgG
GeneID URL（GeneID URL） 57062
ProteinID URL（ProteinID URL） NP_065147.1
抗体类型（Antibody Type） Polyclonal
纯度（Purity） Antigen Affinity Purified
结合物（Conjugate） Unconjugated
物理状态（Physical State） Liquid
产品分类（Product Category） 抗体(Antibody)

我们公司zui大优势是强大的采购，

1：基本什么都能进口，血清，抗体，耗材，还有部分限制进口的，

2： 货品全，现经营过700多个品牌，基本所有生物试剂耗材都可以进口，特别是冷偏的产品那就更有优势，

3：提供加急服务，一般1-2周到货，超过时限加急费全免

4：价格公道，绝大部分价格有优势，当然不能保证100%产品都是,因为意味着没有服务.

5：良好的信誉，大部分客户我们提供货到付款服务，客户包括清华，北大 交大 复旦，中山等100多所大学，ROCHE，阿斯利康，国药 ，fisher等500多家公司

6：我们还是santa,Bethyl Laboratories Inc;Athens Research & Technology, bangs, BBInternational, crystalchem, dianova, Bethyl Laboratories, Inc.  Genebridege; Bethyl Laboratories Biotechnology GmbH; iduron; Bethyl Laboratories； Bethyl Laboratories； neuroprobe; omicronbio; Polysciences; prospecbi; QA-BIO；quickzyme；RESEARCH DIETS, INC；sterlitech；sysy；TriLink BioTechnologies, Inc；worthington-biochem;zyagen;……几十家国外公司代理

7：我们还从事invitrogen，qiagen; abcam ;sigma;neb; roche;merck; rnd; BD; GE; pierce; BioLegend….等*批发

Angiobio心血管产品代理

发表于由

Angiobio心血管产品代理

简要描述：Angiobio是美国一家著名的高科技公司,公司关注血管新生相关技术和产品服务,有相当全的产品提供

详细介绍

产品咨询

Angiobio是美国一家著名的高科技公司,公司关注血管新生相关技术和产品服务,有相当全的产品提供

厂商 目录号 描述  规格 目录价格
AngioBio 11-001 anti-VEGF   200 ug 4760
AngioBio 11-002 anti-Prox1 0.2 ml 4760
AngioBio 11-002P anti-Prox1 100 ug 5100
AngioBio 11-003 anti-human podoplanin 0.2 ml 5100
AngioBio 11-004 anti-hVEGF （JH121） 100 ug 5100
AngioBio 11-008 anti-Flk-1/KDR 100 ug 4250
AngioBio 11-009 anti-human podoplanin 0.2 ml 4760
AngioBio 11-011 anti-Flt-1 100 ug 4250
AngioBio 11-015 anti-Flt-4/VEGFR3 100 ug 5100
AngioBio 11-021 anti-endostatin/CollagenXVIII 100 ug 4250
AngioBio 11-031 anti-human podoplanin 100 ug 5100
AngioBio 11-032 anti-human LYVE-1 100 ug 5100
AngioBio 11-033 anti-mouse podoplanin 100 ug 4760
AngioBio 11-034 anti-mouse LYVE-1 100 ug 5100

美国LifeSensors产品代理信息

发表于由

Life Sensors是一家为药物发现和研究市场提供方法和建立可行性技术方法的生物技术公司。该公司为泛素和泛素蛋白酶体通路研究提供创新性的研究工具而占据世界。其原核和真核蛋白表达系统SUMOpro 和SUMOstar 已被世界范围内的学术、政府研究机构和制药、生物技术公司广泛应用。

部分产品信息如下：

CHOOSE TYPE OF EXPRESSION

E. Coli

Bacterial expression is the usual starting point for expression of heterologous proteins. Bacterial fermentations are inexpensive and can reach high cell densities resulting in high volumetric yields of the target protein. Our SUMO fusion technology is ideally suited for this system.

  • EXPRESSION KITS
  • VECTORS
  • PROTEASE
  • ANTIBODY

Mammalian

Mammalian cell expression has become the system of choice for production of complex, glycosylated biotherapeutic proteins such as antibodies, growth factors and fertility hormones. Our SUMOstar system has been designed for transient transfection studies in HEK293 or CHO cells.

  • EXPRESSION KITS
  • VECTORS
  • PROTEASE
  • ANTIBODY

Insect

Baculovirus/insect cell systems have found wide application for the expression of highly recalcitrant proteins such as protein tyrosine kinases. We have developed a SUMOstar-based system to support expression studies in this system.

  • EXPRESSION KITS
  • VECTORS
  • PROTEASE
  • ANTIBODY

Yeast

As with bacterial expression systems, yeast offer relatively inexpensive growth media and high density fermentation. Furthermore, yeast offer the capability of carrying out limited post-translational modifications such as disulfide bond formation and glycosylation. We offer two yeast expression systems, one for intracellular expression in S. cerevisiae and the second for extracellular expression in P. pastoris.

  • EXPRESSION KITS
  • VECTORS
  • PROTEASE
  • ANTIBODY

Phosphosolutions公司Anti-Actin产品代理

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Phosphosolutions公司Anti-Actin产品代理

简要描述：Phosphosolutions公司Anti-Actin产品代理

详细介绍

产品咨询

 公司概况

 
背景
基因工程– Phosphosolutions是*代可以完整描绘人体的遗传物质序列的企业。
蛋白质体学项目：Phosphosolutions是第二代试图将所有体内蛋白质表达出来的企业。
PhosphoSolutions公司—第三步我们将超越蛋白质体学 进而 专注于磷蛋白质。
 
our focus 专业特色
PhosphoSolutions公司专注于蛋白质组学中的一个（10-20%）含量的小部分磷蛋白质。磷蛋白是监管控制组蛋白质的关键,这一部分是被称为phosphosome蛋白质。磷蛋白被认为是在神经系统疾病如老年痴呆症和癌症方面的关键元素，实质上,phosphosome是蛋白质组学作物的精华。
 
公司目标
简明概述：我们要成为世界上的磷蛋白组的提供者。
方案#1， 特异性磷抗体：首先我们要准备磷蛋白组。在激活或磷酸化状态下磷蛋白组是蛋白质识别研究中的*关键工具。
 
Antibodies 抗体
特异性磷抗体：Detection and quantitation of changes in the state of phosphorylation of specific proteins is of great utility in the quest to establish the function of a given protein and the consequences of its reversible phosphorylation. Two methods commonly used to measure protein phosphorylation and dephosphorylation in cell preparations employ prelabeling with 32Pi or back phosphorylation. These methods continue to be very effective and have advantages for many test systems, but they do have several practical and theoretical limitations （Nestler and Greengard, 1984）. Based in large part on the successful use of short synthetic peptides to produce epitope-targeted antibodies （Lerner, 1982;Sutcliffe et al., 1983）, an immunochemical approach became an attractive alternative for detecting changes in the state of phosphorylation of specific proteins at a specific site. The use of phosphorylation state-specific antibodies takes advantage of the sensitivity and selectivity afforded by immunochemical methodology, combined with relatively simple preparation and potentially broad applications.
The first report of phosphorylation-dependent antibodies appeared in 1981, when polyclonal antibodies that could detect phosphotyrosine-containing proteins were produced by immunization with benzyl phosphonate conjugated to keyhole limpet hemocyanin （KLH） （Ross et al., 1981）. Shortly thereafter, Nairn and colleagues reported the production of serum antibodies that distinguished between the phospho- and dephospho-forms of G-substrate, a protein localized to cerebellar Purkinje cells and phosphorylated by cGMP-dependent protein kinase （Nairn et al., 1982）. A synthetic heptapeptide, Arg-Lys-Asp-Thr-Pro-Ala-Leu, corresponding to a repeated sequence surrounding two phosphorylated threonyl residues in the intact protein, served as antigen. Rabbit antisera against a peptide-KLH conjugate were specific for the dephospho-form of G-substrate. Phospho-specific antibodies were prepared by immunization of rabbits with the purified phosphoprotein, phosphorylated in vitro to a stoichiometry of 2 mol/mol with cGMP-dependent protein kinase. Despite this initial success, other attempts in our laboratory to produce phospho-specific polyclonal antisera by immunization with the phospho-form of intact proteins were not very successful, probably because of two significant factors. First, many phosphorylated proteins are believed to undergo rapid dephosphorylation during immunization, regardless of the route of injection, leading to the loss of the desired phospho-epitope. Second, holoproteins generally contain multiple immunogenic epitopes; this decreases the probability that colonal dominance for a phospho-specific epitope will be obtained.
Taking a more direct approach utilizing phosphorylated and unphosphorylated forms of synthetic phosphopeptides, we developed a general protocol for the production of phosphorylation state-specific antibodies for substrates with established site（s） of phosphorylation （Czernik et al., 1991））. In early stages of our development of this methodology, phosphopeptides were routinely prepared by enzymatic phosphorylation （Czernik et al., 1991）. Although this approach remains perfectly valid today, the preparation of synthetic phosphopeptides using Fmoc derivatives of phosphoamino acids has become the state-of-the-art （Czernik et al., 1995;Czernik et al., 1996）. Likewise, we have examined the use of both polyclonal and monoclonal techniques for antibody production. Given the high success rate that we and others have obtained with the polyclonal technique, it has become the method of choice, because it is an easier and less costly method for the average laboratory. However, when appropriate, this approach can be readily adapted for monoclonal antibody production.
参考文献
1. Czernik AJ, Girault J-A, Nairn AC, Chen J, Snyder G, Kebabian J, Greengard P （1991） Production of phosphorylation state-specific antibodies. Methods Enzymol 201: 264-283.
2. Czernik AJ, Mathers J, Mische SM （1997） Phosphorylation state-specific antibodies. Neuromethods: Regulatory Protein Modification: Techniques & Protocols 30: 219-250.
3. Czernik AJ, Mathers J, Tsou K, Greengard P, Mische SM （1995） Phosphorylation state-specific antibodies: preparation and applications. Neuroprotocols 6: 56-61.
4. Lerner, R. A. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299, 593-596. 1982.
5. Nairn AC, Detre JA, Casnellie JE, Greengard P （1982） Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein. Nature （Lond ） 299: 734-736.
6. Nestler, E. J. and Greengard, P. Protein Phosphorylation in the Nervous System. Nestler and Greengard. Protein Phosphorylation in the Nervous System. [8], 255-299. 1984. New York, Wiley. 
8. Sutcliffe JG, Shinnick TM, Green N, Lerner RA （1983） Antibodies that react with predetermined sites on proteins. Science 219: 660-666.
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125-ACT $275.00 ascites fluid WB, IF, IHC 100 ul

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简要描述：Finnzymes为高效的PCR、RT-PCR和qPCR提供全面的解决方案。其在DNA聚合酶方面的专业技术显著提高了DNA聚合酶的性能，使PCR的过程更快速更精确。其所具备的快速、可重复地扩增和定量特殊DNA序列的能力施惠于各种应用领域，包括基因组学基础研究、遗传检测、法医鉴定以及食品检测等。

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Finnzymes为高效的PCR、RT-PCR和qPCR提供全面的解决方案。其在DNA聚合酶方面的专业技术显著提高了DNA聚合酶的性能，使PCR的过程更快速更精确。其所具备的快速、可重复地扩增和定量特殊DNA序列的能力施惠于各种应用领域，包括基因组学基础研究、遗传检测、法医鉴定以及食品检测等。
 

Legacy Cat. No. Product name Amount （UOM） 2012 List Price
Finnzymes ASF-0010 Adhesive Seal for Piko/PikoReal 100 seals ￥2,690
Finnzymes ASF-0020 Adhesive Seal for Piko/PikoReal 400 seals ￥2,266
Finnzymes F-120L Phire Hot Start DNA Polymerase 1000 x 50ul ￥3,757
Finnzymes F-120S Phire Hot Start DNA Polymerase 200 x 50ul ￥939
Finnzymes F-122L Phire Hot Start II DNA Polymerase 1000 x 50ul ￥3,757
Finnzymes F-122S Phire Hot Start II DNA Polymerase 200 x 50ul ￥939
Finnzymes F-130 Phire Plant Direct PCR Kit 200 x 50ul ￥1,921
Finnzymes F-140 Phire Animal Tissue Direct PCR 200 x 50ul ￥2,433
Finnzymes F-150 Phusion Human Specimen Direct 200 x 50ul ￥1,810
Finnzymes F-185L Harris Uni-Core/25-0.50mm Blue Bag of 25 ￥2,305
Finnzymes F-185S Harris Uni-Core/5-0.50mm Blue Bag of 5 ￥602
Finnzymes F-190S Harris Cutting Mat 6.4 x 7.6 cm, Bag of 5 Bag of 5 ￥376
Finnzymes F-203L Terminal Transferase （TdT） 2500 U ￥1,800
Finnzymes F-203S Terminal Transferase （TdT） 500 U ￥450
Finnzymes F-400L DyNAmo SYBR Green qPCR Kit 500 x 20ul ￥960
Finnzymes F-400S DyNAmo SYBR Green qPCR Kit 100 x 20ul ￥240
Finnzymes F-410L DyNAmo HS SYBR Green qPCR Kit 500 x 20ul ￥1,920
Finnzymes F-410S DyNAmo HS SYBR Green qPCR Kit 100 x 20ul ￥480
Finnzymes F-410XL DyNAmo HS SYBR Green qPCR Kit 2500 x 20 ul ￥7,600
Finnzymes F-415L DyNAmo Flash SYBR Green qPCR Kit 500 x 20ul ￥2,100
Finnzymes F-415S DyNAmo Flash SYBR Green qPCR Kit 100 x 20ul ￥525
Finnzymes F-415XL DyNAmo Flash SYBR Green qPCR Kit 2500 x 20 ul ￥8,000
Finnzymes F-416L DyNAmo ColorFlash SYBR Green qPCR Kit 500 x 20ul ￥2,100
Finnzymes F-416S DyNAmo ColorFlash SYBR Green qPCR Kit 100 x 20ul ￥525
Finnzymes F-416XL DyNAmo ColorFlash SYBR Green qPCR Kit 2500 x 20 ul ￥8,000
Finnzymes F-420L DyNAmo Capillary SYBR Green qPCR Kit  500 x 20ul ￥5,336
Finnzymes F-420S DyNAmo Capillary SYBR Green qPCR Kit  100 x 20ul ￥1,332
Finnzymes F-430L DyNAmo SYBR Green 2-step qRT-PCR Kit  100 x RT, 500 x qPCR ￥3,200
Finnzymes F-430S DyNAmo SYBR Green 2-step qRT-PCR Kit  20 x RT, 100 x qPCR ￥800
Finnzymes F-440L DyNAmo Capillary SYBR Green 2-step qRT-PCR Kit  100 x RT, 500 x qPCR ￥6,659
Finnzymes F-440S DyNAmo Capillary SYBR Green 2-step qRT-PCR Kit  20 x RT, 100 x qPCR ￥1,665
Finnzymes F-450L DyNAmo Probe qPCR Kit  500 x 20ul ￥2,100
Finnzymes F-450S DyNAmo Probe qPCR Kit  100 x 20ul ￥525
Finnzymes F-450XL DyNAmo Probe qPCR Kit  2500 x 20 ul ￥7,900
Finnzymes F-455L DyNAmo Flash Probe qPCR Kit  500 x 20ul ￥2,205
Finnzymes F-455S DyNAmo Flash Probe qPCR Kit  100 x 20ul ￥545
Finnzymes F-455XL DyNAmo Flash Probe qPCR Kit  2500 x 20 ul ￥8,300
Finnzymes F-456L DyNAmo ColorFlash Probe qPCR Kit  500 x 20ul ￥2,205
Finnzymes F-456S DyNAmo ColorFlash Probe qPCR Kit  100 x 20ul ￥545
Finnzymes F-456XL DyNAmo ColorFlash Probe qPCR Kit  2500 x 20 ul ￥8,300
Finnzymes F-460L DyNAmo Probe 2-step qRT-PCR Kit  100 x RT, 500 x qPCR ￥4,800
Finnzymes F-460S DyNAmo Probe 2-step qRT-PCR Kit  20 x RT, 100 x qPCR ￥1,160
Finnzymes F-470L DyNAmo cDNA Synthesis Kit  100 Rxns ￥2,500
Finnzymes F-470S DyNAmo cDNA Synthesis Kit  20 Rxns ￥600
Finnzymes F-480L DyNAmo SNP Genotyping Master Mix  1000 x 25 ul ￥8,300
Finnzymes F-480S DyNAmo SNP Genotyping Master Mix  200 x 25 ul ￥2,090
Finnzymes F-480XL DyNAmo SNP Genotyping Master Mix  4000 x 25 ul ￥26,760
Finnzymes F-500L DyNAzyme DNA Polymerase （native） 500 U ￥2,142
Finnzymes F-500S DyNAzyme DNA Polymerase （native） 100 U ￥500
Finnzymes F-501L DyNAzyme II DNA Polymerase 1000 U ￥1,200
Finnzymes F-501S DyNAzyme II DNA Polymerase 250 U ￥375
Finnzymes F-502L DyNAzyme DNA Polymerase with Mg-free buffer 500 U ￥2,232
Finnzymes F-502S DyNAzyme DNA Polymerase with Mg-free buffer 100 U ￥522
Finnzymes F-503L DyNAzyme II DNA Polymerase with Mg-free buffer 1000 U ￥1,320
Finnzymes F-503S DyNAzyme II DNA Polymerase with Mg-free buffer 250 U ￥412
Finnzymes F-504L DyNAzyme II Hot Start DNA Polymerase 1000 U ￥3,400
Finnzymes F-504S DyNAzyme II Hot Start DNA Polymerase 200 U ￥1,000
Finnzymes F-505L DyNAzyme EXT DNA Polymerase 1000 U ￥3,000
Finnzymes F-505S DyNAzyme EXT DNA Polymerase 200 U ￥750
Finnzymes F-510L Mg-free DyNAzyme Buffer 4 x 1,5 ml ￥102
Finnzymes F-510S Mg-free DyNAzyme Buffer 2 x 1,5 ml ￥68
Finnzymes F-511L Optimized DyNAzyme Buffer 4 x 1,5 ml ￥102
Finnzymes F-511S Optimized DyNAzyme Buffer 2 x 1,5 ml ￥68
Finnzymes F-512L DyNAzyme EXT Buffer Pack 4 x 1,5 ml ￥120
Finnzymes F-512S DyNAzyme EXT Buffer Pack 2 x 1,5 ml ￥77
Finnzymes F-514L Optimized DyNAzyme EXT Buffer 4 x 1,5 ml ￥100
Finnzymes F-514S Optimized DyNAzyme EXT Buffer 2 x 1,5 ml ￥67
Finnzymes F-516L Optimized DyNAzyme buffer, detergent-free 4 x 1,5 ml ￥102
Finnzymes F-516S Optimized DyNAzyme buffer, detergent-free 2 x 1,5 ml ￥68
Finnzymes F-517L Optimized DyNAzyme EXT Buffer, detergent-free 4 x 1,5 ml ￥81
Finnzymes F-517S Optimized DyNAzyme EXT Buffer, detergent-free 2 x 1,5 ml ￥54
Finnzymes F-518L Phusion HF Buffer Pack 4 x 1,5 ml ￥120
Finnzymes F-518S Phusion HF Buffer Pack 2 x 1,5 ml ￥77
Finnzymes F-519L Phusion GC Buffer Pack 4 x 1,5 ml ￥120
Finnzymes F-519S Phusion GC Buffer Pack 2 x 1,5 ml ￥77
Finnzymes F-520L Detergent-free Phusion HF Buffer Pack 4 x 1,5 ml ￥120
Finnzymes F-520S Detergent-free Phusion HF Buffer Pack 2 x 1,5 ml ￥77
Finnzymes F-521L Detergent-free Phusion GC Buffer Pack 4 x 1,5 ml ￥120
Finnzymes F-521S Detergent-free Phusion GC Buffer Pack 2 x 1,5 ml ￥77
Finnzymes F-522L DyNAzyme II Hot Start Reaction Buffer 4 x 1,5 ml ￥120
Finnzymes F-522S DyNAzyme II Hot Start Reaction Buffer 2 x 1,5 ml ￥77
Finnzymes F-524L Phire Reaction Buffer 4 x 1,5 ml ￥120
Finnzymes F-524S Phire Reaction Buffer 2 x 1,5 ml ￥77
Finnzymes F-525L Detergent-free 5x Phire Reaction Buffer 4 x 1,5 ml ￥120
Finnzymes F-525S Detergent-free 5x Phire Reaction Buffer 2 x 1,5 ml ￥77
Finnzymes F-530L Phusion High-Fidelity DNA Polymerase 500U ￥3,517
Finnzymes F-530S Phusion High-Fidelity DNA Polymerase 100U ￥879
Finnzymes F-531L Phusion High-Fidelity PCR Master Mix with HF Buffer 500 x 50ul ￥5,703
Finnzymes F-531S Phusion High-Fidelity PCR Master Mix with HF Buffer 100 x 50ul ￥1,426
Finnzymes F-532L Phusion High-Fidelity PCR Master Mix with GC Buffer 500 x 50ul ￥5,703
Finnzymes F-532S Phusion High-Fidelity PCR Master Mix with GC Buffer 100 x 50ul ￥1,426
Finnzymes F-540L Phusion Hot Start DNA Polymerase 500U ￥4,269
Finnzymes F-540S Phusion Hot Start DNA Polymerase 100U ￥1,067
Finnzymes F-541 Phusion Site-Directed Mutagenesis Kit 20 Rxns ￥1,195
Finnzymes F-546L Phusion RT-PCR Kit 100 Rxns ￥3,244
Finnzymes F-546S Phusion RT-PCR Kit 20 Rxns ￥811
Finnzymes F-547L Phusion Blood Direct PCR Kit 500 Rxns ￥3,244
Finnzymes F-547S Phusion Blood DirectPCR Kit 100 Rxns ￥811
Finnzymes F-548L Phusion Flash High-Fidelity PCR Master Mix 500 Rxns ￥2,664
Finnzymes F-548S Phusion Flash High-Fidelity PCR Master Mix 100 Rxns ￥666
Finnzymes F-549L Phusion Hot Start II High-Fidelity DNA Polymerase 500U ￥4,269
Finnzymes F-549S Phusion Hot Start II High-Fidelity DNA Polymerase 100U ￥1,067
Finnzymes F-550L DyNAzyme I DNA Polymerase Kit 500 units ￥1,950
Finnzymes F-550S DyNAzyme I DNA Polymerase Kit 100 units ￥415
Finnzymes F-551L DyNAzyme II DNA Polymerase Kit 500 units ￥1,638
Finnzymes F-551S DyNAzyme II DNA Polymerase Kit 100 units ￥378
Finnzymes F-552L DyNAzyme EXT PCR Kit  1000 Rxns ￥3,600
Finnzymes F-552S DyNAzyme EXT PCR Kit  200 Rxns ￥900
Finnzymes F-553L Phusion High-Fidelity PCR Kit 200 x 50ul ￥1,878
Finnzymes F-553S Phusion High-Fidelity PCR Kit 50 x 50ul ￥555
Finnzymes F-570L AMV Reverse Transcriptase 2 500 U ￥3,150
Finnzymes F-570S AMV Reverse Transcriptase 500 U ￥720
Finnzymes F-572L M-MuLV Reverse Transcriptase, RNase H- 50 000 U ￥3,380
Finnzymes F-572S M-MuLV Reverse Transcriptase, RNase H- 10 000 U ￥854
Finnzymes F-580L RobusT I RT-PCR Kit 100 rxn ￥2,250
Finnzymes F-580S RobusT I RT-PCR Kit 20 rxn ￥540
Finnzymes F-590L RobusT II RT-PCR Kit 100 rxn ￥2,250
Finnzymes F-590S RobusT II RT-PCR Kit 20 rxn ￥540
Finnzymes F-602L PowerCut Dicer 300 U ￥2,700
Finnzymes F-602S PowerCut Dicer 60 U ￥630
Finnzymes F-610 Replicator RNAi Kit 40 rxn ￥2,700
Finnzymes F-611L Phi6 RNA Replicase 300 U ￥3,240
Finnzymes F-611S Phi6 RNA Replicase 60 U ￥720
Finnzymes F-630 Phi6 dsRNA 20 ug ￥540
Finnzymes F-701 Mutation Generation System Kit 10 rxns ￥1,800
Finnzymes F-702 Template Generation System II Kit 20 rxns ￥1,800
Finnzymes F-703 Stop Generation System Kit 10 rxns ￥1,800
Finnzymes F-750 MuA Transposase 20 ul ￥1,152
Finnzymes F-750C MuA Transposase 5x concentrate 20 ul ￥5,220
Finnzymes F-760 Entranceposon （M1-Cam） 10 ul ￥351
Finnzymes F-762 Entranceposon （M1-Kan） 10 ul ￥351
Finnzymes F-764 Entransceposon （TetR） 20 ul ￥351
Finnzymes F-765 pEntranceposon （Cam） 40 ul ￥540
Finnzymes F-766 pEntranceposon（KanR） 40 ul ￥540
Finnzymes F-767 pEntranceposon （Tet） 40 ul ￥540
Finnzymes F-771 Entranceposon （SupF） 20 ul ￥351
Finnzymes F-772 Entranceposon （SupF-2） 20 ul ￥351
Finnzymes F-773 pEntranceposon （SupF） 40 ul ￥540
Finnzymes F-774 Entranceposon （LacZ） 20 ul ￥351
Finnzymes F-775 Entranceposon （LacZ-PT） 20 ul ￥351
Finnzymes F-778 Entranceposon （Cam-3） 20 ul ￥351
Finnzymes F-779 Entranceposon （Kan-3） 20 ul ￥351
Finnzymes F-784 Entranceposon （TetR-3） 20 ul ￥351
Finnzymes PRK0963 96-well Preparation Rack with cover, 5 pcs 5 pcs ￥342
Finnzymes PRK1383 384-well Aluminium Support Rack 1 ￥1,699
Finnzymes PRK1963 96-well Aluminium Support Rack 1 ￥1,699
Finnzymes SFR-0241 Piko PCR 24-well plate frames 50 frames ￥500
Finnzymes SFR-0961 Piko PCR 96-well plate frames 50 frames ￥500
Finnzymes SPL0240 24-well Piko PCR plate, Clear 200 plates ￥4,180
Finnzymes SPL0241 24-well Piko PCR plate, White 200 plates ￥4,180
Finnzymes SPL0960 96-well Piko PCR plate, Clear 200 plates ￥4,180
Finnzymes SPL0961 96-well Piko PCR plate, White 200 plates ￥4,180
Finnzymes TCS-1080 8-Cap Strips, Flat, Optically Clear 120 strips ￥4,180
Finnzymes TUC0010 UTW 0.1ml Individual Tubes with Flat Cap （Clear） 960 pcs ￥1,416
Finnzymes TUC0011 UTW 0.1ml Individual Tubes with Flat Cap （White） 960 pcs ￥1,416
Finnzymes F-180L Harris Uni-Core/25-0.50mm Blue bag of 25 ￥2,997
Finnzymes F-180S Harris Uni-Core/5-0.50mm Blue bag of 5 ￥666
Finnzymes PIP-2496 Piko Plate Illuminator 1 ￥5,051
Finnzymes TUC0080 UTW 8-Tube Strips with Caps （Clear） 120 pcs ￥1,332
Finnzymes TUC0081 UTW Bi-Color 8-Tube Strip with Caps （White） 120 pcs ￥1,480
Finnzymes F-400RL DyNAmo SYBR Green qPCR Kit with ROX 500 x 20ul ￥761
Finnzymes F-400RS DyNAmo SYBR Green qPCR Kit with ROX 100 x 20ul ￥195
Finnzymes F-508L DyNAzyme II PCR Master Mix 1000 x 50 ul Rxns ￥1,912
Finnzymes F-508S DyNAzyme II PCR Master Mix 200 x 50 ul Rxns ￥425