毕赤酵母表达系统说明书


毕赤酵母表达系统说明书

You can put the power of P. pastoris into your laboratory with a complete Pichia Expression Kit or with individual Pichia expression vectors. Three Pichia Expression Kits are available, each of which includes vectors, P. pastoris strains, reagents for transformation, sequencing primers, media, and a comprehensive manual (Table 3). In addition, the pPICZ, pGAPZ, and pPIC6 vectors are available separay (see insert for ordering information). Choose the kit or vector that best meets your research needs and take advantage of the proven strength of P. pastoris.

Table 3—Pichia expression kit components.

EasySelect™ Pichia

Expression Kit

Multi-Copy Pichia

Expression Kit

Original Pichia

Expression Kit

Vectors

pPICZ A, B, C 

(20 µg each)

pPICZα A, B, C 

(20 µg each)

pPIC9K (20 µg)

pPIC3.5K (20 µg)

pAO815 (20 µg)

pPIC9 (10 µg) 

pPIC3.5 (10 µg)

pHIL-D2 (10 µg)

pHIL-S1 (10 µg)

Strains

X-33 (Mut+

, His+

GS115 (Mut+

KM71 (MutS

GS115/pPICZ/lacZ 

(control)

GS115 (Mut+

KM71 (MutS

GS115/β-gal (control)

GS115/albumin (control)

GS115 (Mut+

KM71 (MutS

GS115/β-gal (control)

GS115/albumin (control)

Transformation 

reagents

Pichia EasyComp™ Kit

(120 transformations)

Spheroplast Module

(50 transformations)

Spheroplast Module

(50 transformations)

Sequencing

primers

5´ AOX1

3´ AOX1

α-factor

5´ AOX1

3´ AOX1

α-factor

5´ AOX1

3´ AOX1

α-factor

Media and 

supplements

YP base medium 

 (2 pouches)*

YP base agar 

 (2 pouches)*

Yeast Nitrogen Base 

 (1 pouch)†

 Zeocin™ (250 mg)

YP base medium 

 (2 pouches)*

YP base agar

 (2 pouches)*

Yeast Nitrogen Base 

 (1 pouch)†

YP base medium 

 (2 pouches)*

YP base agar

 (2 pouches)*

Yeast Nitrogen Base

 (1 pouch)†