pTK-hyg
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VSC0467 | pTK-hyg | Clontech | 四环素调控系统 |
Description
pTK-Hyg is a selection vector that confers hygromycin resistance in mammalian cells for the
selection of stably transformed cells. pTK-Hyg is especially useful for selection of double-stable
cell lines using the Tet-On® or Tet-Off® Gene Expression Systems because the absence of an
enhancer element on pTK-Hyg prevents the unwanted activation of pTRE- and pBI-derived
plasmids upon cointegration into the host cell’s genome.
Use
pTK-Hyg can be cotransfected into the host cell line with an expression plasmid that contains
a gene of interest using any standard transfection technique. Most mammalian cell lines
require 200 µg/ml of Hygromycin to select for stably transformed cells.
Location of Features
• HSV TK polyA signals: 1473–1478 & 1486–1491 （complementary）
• Hygr
（hygromycin resistance gene）: 1537–2574 （complementary）
• P
HSV TK （HSV TK promoter）: 2588–2835 （complementary）
• pUC origin ofreplication: 3152–3795
• Ampr
（ampicillin resistance gene; beta-lactamase）: 3943–4803
Propagation in E. coli
• Suitable host strains: DH™ and other general purpose strains.
• Selectable marker: plasmid confers resistance to ampicillin （50 µg/ml） on E. coli hosts.
• E. coli replication origin: pUC