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Collagenase NB 4 Standard Grade from Clostridium histolyticum
(Clostridiopeptidase A)
EC 3.4.24.3 • Mr ca. 70 000 – 120 000 (collagenases) • CAS [9001-12-1]
Contains class I and class II collagenase (1) and a balanced ratio of proteolytic side activities.
PZ activity (Wünsch): ≥ 0.10 U/mg
Suitable for different applications in tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.
As Collagenase NB 4 Standard Grade has comparable enzymatic properties to Collagenase NB 5 Sterile Grade (cat. no. 17459) and Collagenase NB 6 GMP Grade (cat. no. 17458), this enzyme might also be used in order to establish applications which have to meet sterile or GMP requirements like tissue engineering and transplantation.
Unit definition: 1 U according to Wünsch (2) catalyzes the hydrolysis of
1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.
References:
- Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091
- Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51
DANGER |
Hazard Statements | H315 – H317 – H319 – H334 – H335 |
Precautions | P280 |
Reaction | P302 +P352 – P304 +P340 – P305 +P351 +P338 – P342 +P311 – P362 – P362 +P364 |
EINECS: 232-582-9 • WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C
货号 | 规格 |
17454.02 | 500 mg |
17454.01 | 1 g |
17454.03 | 5 g |