Merck蛋白抽提产品
简要描述：BugBuster蛋白抽提试剂用于温和破坏大肠杆菌细胞壁以释放活性蛋白。作为激烈的机械法（如弗式压榨法和超声波法）的替代方法，这种试剂处理起来非常简便、快速，且成本低廉，适合制备表达蛋白用于下游的纯化等应用。
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Merck蛋白抽提
MERCK蛋白抽提
1、BugBuster®蛋白抽提试剂——尤其适用于GST融合蛋白的抽提
BugBuster蛋白抽提试剂用于温和破坏大肠杆菌细胞壁以释放活性蛋白。作为激烈的机械法（如弗式压榨法和超声波法）的替代方法，这种试剂处理起来非常简便、快速，且成本低廉，适合制备表达蛋白用于下游的纯化等应用。
2、BugBuster Master Mix预混蛋白抽提试剂
BugBuster Master Mix是将BugBuster蛋白抽提试剂，Benzonase核酸酶和rLysozyme™溶菌酶溶液按*化配比混合好的一种非常方便使用的蛋白抽提试剂，有助于在*化条件下获得可溶活性蛋白。
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Product
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Size
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Cat.No.
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Price（￥）
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BugBuster® Protein Extraction Reagent
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100 ml
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70584-3
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750.00
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500 ml
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70584-4
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3,789.00
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BugBuster® Master Mix
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100 ml
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71456-3
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1,700.00
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500 ml
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71456-4
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5,200.00
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CytoBuster™ Protein Extraction Reagent
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50 ml
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71009-3
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824.00
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250 ml
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71009-4
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3,122.00
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Comparison of E. coli lysis methods
50 ml samples of an induced 500 ml culture of BL21（DE3） containing pET-41a（+） encoding GST were harvested by centrifugation and resuspended in 2 ml 1X PBS, another commercially available protein extraction reagent, or BugBuster Reagent. The sample in PBS was sonicated with 10 pulses at 50% duty for 30 s total. Samples in lysis reagent were treated according to their respective protocols. Extracts were clarified by centrifugation and assayed for GST enzymatic activity.
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3、Benzonase® 核酸酶——赛多利斯病毒纯化试剂盒推荐使用
应用范围
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² 蛋白抽提操作中降低样品粘度
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² 双向电泳中样品制备过程
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² 重组蛋白样品制备中去除核酸污染
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Product
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Size
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Cat.No.
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Price（￥）
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Benzonase® Nuclease, Purity > 90%
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10 ku
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70746-3
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1,200.00
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2.5 ku
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70746-4
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400.00
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Viscosity reduction by Benzonase Nuclease
E. coli BL21（DE3） cells containing a pET construct were suspended in BugBuster Reagent （5 ml/g wet weight）. Aliquots of the suspension were treated with the indicated amounts of Benzonase Nuclease for 10 min at
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