Tubulin-Tracker Red (抗体法微管红色荧光探针)
产品编号: C1050
产品包装:40μl
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40μl
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Tubulin-Tracker Red (抗体法微管红色荧光探针)(C1050)
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价格: ¥ 906.00
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| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| C1050 | Tubulin-Tracker Red (抗体法微管红色荧光探针) | 40μl | 906.00元 |
Tubulin-Tracker Red是一种Tubulin红色荧光探针，可以用于培养细胞或组织切片的Tubulin特异性荧光染色。
Tubulin-Tracker Red探针为荧光染料Alexa Fluor 555标记的抗α-Tubulin小鼠单克隆抗体(克隆号为DM1A)，可以识别α-Tubulin的425-450aa。可以用于人、小鼠、大鼠、牛、猪、豚鼠和禽类(avian)样品α-Tubulin的检测。最大激发波长为555nm，最大发射波长为565nm。Alexa Fluor 555的荧光光谱和Cy3非常接近，可以用Cy3的检测条件进行检测。
本产品可以用于细胞或组织内的微管(microtubule)的荧光检测。可以用于荧光显微镜观察，也可用于流式细胞仪检测。使用本产品染色细胞内微管的效果请参考图1。
图1.Tubulin-Tracker Red对NRK-52E细胞(大鼠肾小管上皮细胞)的染色效果。图A为明场下大鼠肾小管上皮细胞形态图；绿色荧光为Actin-Tracker Green-488 (C2201S)染色的微丝(图B)，红色荧光为Tubulin-Tracker Red染色的微管(图C)，图D为Tubulin-Tracker Red、Actin-Tracker Green-488及Hoechst 33342三重染色的叠加图。实测数据会因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。
本产品使用时的推荐稀释比例为1:50-100，共可以配制2-4ml染色液。如果每个片子需要使用100µl染色工作液，每个包装的本产品足够用于20-40个片子的染色。
包装清单：
| 产品编号 | 产品名称 | 包装 |
| C1050 | Tubulin-Tracker Red(抗体法微管红色荧光探针) | 40µl |
| － | 说明书 | 1份 |
保存条件：
-20℃避光保存，1年有效。
注意事项：
对于微量的液体，每次使用前先离心数秒钟，使液体充分沉降到管底。
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
需自备盖玻片和载玻片(可以向碧云天订购)。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1.固定细胞或组织切片的荧光染色：
a.用PBS洗涤细胞或组织切片2次。
b.用碧云天的免疫染色固定液(P0098)或PBS配制的3.7%甲醛溶液室温固定细胞或组织切片约10-20分钟。
c.用碧云天的免疫染色洗涤液(P0106)或含0.1% Triton X-100的PBS洗涤2-3次，每次5分钟。
d.用碧云天的免疫荧光染色二抗稀释液(P0108)或含有1-5%BSA和0.1% Triton X-100的PBS按照1:50-100的比例稀释Tubulin-Tracker Red，例如4µl Tubulin-Tracker Red用0.2ml或0.4ml稀释液稀释，稀释后的溶液即为Tubulin-Tracker Red染色工作液。稀释比例可以根据实际染色效果进行适当调整。
e.把Tubulin-tracker Red染色工作液按照约每个片子100µl的比例滴加到片子上，室温避光孵育30-60分钟。为防止蒸发，孵育时最好将片子置于载玻片染色盒中，载玻片染色盒(FSR958)可以向碧云天订购。
f.用碧云天的免疫染色洗涤液(P0106) 或含0.1% Triton X-100的PBS洗涤2-4次，每次5分钟。
g.随后可以直接用荧光显微镜进行观察。也可以用抗荧光淬灭封片液封片后保存和观察。
2.流式细胞仪检测：
a.每个样品收集约20-50万细胞。
b.PBS洗涤一次。
c.用碧云天的免疫染色固定液(P0098)或PBS配制的3.7%甲醛溶液室温固定细胞约10-20分钟。
d.用碧云天的免疫染色洗涤液(P0106)或含0.1% Triton X-100的PBS洗涤2-3次，每次5分钟。
e.用碧云天的免疫荧光染色二抗稀释液(P0108)或含有1-5%BSA和0.1% Triton X-100的PBS重悬细胞，然后按照1:50-100的比例加入Tubulin-Tracker Red。例如1µl Tubulin-Tracker Red加入到50-100µl细胞悬液中。Tubulin-Tracker Red稀释比例可以根据实际染色效果进行适当调整。
f.室温避光孵育1小时。
g.用碧云天的免疫染色洗涤液(P0106)或含0.1% Triton X-100的PBS洗涤2-3次，每次5分钟。随后即可进行流式细胞仪分析。
相关产品：
| 产品编号 | 产品名称 | 包装 |
| C1002 | DAPI | 5mg/ml×0.2ml |
| C1005/C1006 | DAPI染色液 | 10ml/50ml |
| C1011 | Hoechst 33258 | 10mg |
| C1017/C1018 | Hoechst 33258染色液 | 10ml/50ml |
| C1022 | Hoechst 33342 | 10mg |
| C1025/C1026 | Hoechst 33342染色液 | 10ml/50ml |
| C1027/C1028/C1029 | Hoechst 33342活细胞染色液(100X) | 0.1ml/0.5ml/3ml |
| C1033 | Actin-Tracker Green (微丝绿色荧光探针) | 0.2ml |
| C1036 | DiI (细胞膜红色荧光探针) | 10mg |
| C1038 | DiO (细胞膜绿色荧光探针) | 10mg |
| C1039-10mg | DiD (细胞膜远红外荧光探针) | 10mg |
| C1041 | ER-Tracker Red (内质网红色荧光探针) | 20μl |
| C1042S | ER-Tracker Green (内质网绿色荧光探针) | 20μl |
| C1043 | Golgi-Tracker Red (高尔基体红色荧光探针) | 1mg |
| C1045S | Golgi-Tracker Green (高尔基体绿色荧光探针) | 1mg |
| C1046 | Lyso-Tracker Red (溶酶体红色荧光探针) | 50μl |
| C1047S | Lyso-Tracker Green (溶酶体绿色荧光探针) | 50μl |
| C1048 | Mito-Tracker Green (线粒体绿色荧光探针) | 50μg |
| C1049B-50μg | Mito-Tracker Red CMXRos (线粒体红色荧光探针) | 50μg |
| C1049B-250μg | Mito-Tracker Red CMXRos (线粒体红色荧光探针) | 50μg×5 |
| C1050 | Tubulin-Tracker Red (抗体法微管红色荧光探针) | 40μl |
| C1051S | Tubulin-Tracker Green (抗体法微管绿色荧光探针) | 40μl |
| C1991S | 细胞膜红色荧光染色试剂盒(DiI) | 200-1000次 |
| C1993S | 细胞膜绿色荧光染色试剂盒(DiO) | 200-1000次 |
| C1995S | 细胞膜远红外荧光染色试剂盒(DiD) | 200-1000次 |
| C2005 | JC-1 | 1mg |
| C2007 | Rhodamine 123 | 5mg |
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