SYSY 131 002 说明书

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VGAT (vesicular GABA transporter, VIAAT)

GABA transporter in the membrane of synaptic vesicles

Fact Sheet Cat. No. 131 002

Polyclonal rabbit antiserum, antibody 1 cytoplasmic domain
Cat. No. 131 002
crude antiserum
200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 (AP staining, see remarks) blot 
IP: yes 
ICC: 1 : 100 up to 1 : 1000 image 
IHC: 1 : 500 up to 1 : 3000 image 
IHC-P: yes
Immunogen Synthetic peptide AEPPVEGDIHYQR (aa 75 – 87 in rat) coupled to key-hole limpet hemocyanin via an added N- terminal cysteine.
Reactivity Human, rat, mouse, monkey; other species not tested yet.
Specificity Specific for VGAT (K.O. verified).
Remarks WB: VGAT aggregates after boiling, making it necessary to run SDS-PAGE only withnon-boiled samples.

 

VGAT(水泡GABA转运蛋白,VIAAT)

突触小泡膜中的GABA转运蛋白

一般信息

The vesicular GABA transporter VGAT is responsible for uptake and storage of GABA and glycine by synaptic vesicles in the central nervous system. For this reason it is frequently referred to as thevesicular inhibitory aminoacid transporter VIAAT. It is different from the plasma membrane transporters in that it is driven by a proton electrochemical gradient across the vesicle membrane. So far, only one isoform is known. VGAT is currently the best marker for inhibitory nerve terminals.

 

相关产品信息如下:

Cat. No. Antigen Product Applications Clone Qty.
 
131 011 VGAT 
cytoplasmic domain
mouse monoclonal, purified IgG, unlabeled 
K.O. verified
WB IP ICC IHC IHC-P EM 117G4 100µg
131 011C2 VGAT 
cytoplasmic domain
mouse monoclonal, purified IgG, Oyster 488-labeled 
K.O. verified
ICC 117G4 100µg
131 011C3 VGAT 
cytoplasmic domain
mouse monoclonal, purified IgG, Oyster 550-labeled 
K.O. verified
ICC IHC 117G4 100µg
131 011BT VGAT 
cytoplasmic domain
mouse monoclonal, purified IgG, biotin-labeled 
K.O. verified
WB IP ICC IHC 117G4 100µg
131 002 VGAT 
cytoplasmic domain
rabbit polyclonal, antiserum 
antibody 1, K.O. verified
WB IP ICC IHC IHC-P   200µl
131 003 VGAT 
cytoplasmic domain
rabbit polyclonal, affinity purified 
antibody 1, K.O. verified
WB IP ICC IHC EM   50µg
131 006 VGAT 
cytoplasmic domain
chicken polyclonal, purified IgY ICC IHC IHC-P   50µg
131-0P VGAT 
cytoplasmic domain
control peptide     100µg
131 013 VGAT 
cytoplasmic domain
rabbit polyclonal, affinity purified 
antibody 2, K.O. verified
WB IP ICC IHC EM   50µg
131 004 VGAT 
cytoplasmic domain
Guinea pig polyclonal, antiserum, K.O. verified WB ICC IHC IHC-P EM   100µl
131-0GP VGAT 
cytoplasmic domain
control protein     100µg
131 103 VGAT 
lumenal domain
rabbit polyclonal, affinity purified, unlabeled 
K.O. verified
WB IP ICC IHC   50µg
131 103C2 VGAT 
lumenal domain
rabbit polyclonal, affinity purified, Oyster 488-labeled 
K.O. verified
ICC IHC   50µg
131 103C3 VGAT 
lumenal domain
rabbit polyclonal, affinity purified, Oyster 550-labeled 
K.O. verified
ICC IHC   50µg
131 103C5 VGAT 
lumenal domain
rabbit polyclonal, affinity purified, Oyster 650-labeled 
K.O. verified
ICC IHC   50µg
131 103CpH VGAT 
lumenal domain
rabbit polyclonal, affinity purified, CypHer5E-labeled 
K.O. verified
ICC IHC   50µg