货号：L6030-W-L
品名：WGS Ligase （WGS连接酶）
产品描述：
WGS连接酶针对WGS碎片化后的连接进行了优化。它催化在双链DNA或RNA的末端5'磷酸和3'羟基之间形成磷酸二酯键。该酶有效连接平端和粘性末端，并修复双链RNA，RNA或DNA / RNA杂合体中的单链缺口（1）。
|
规格： |
24 Reactions |
|
浓度： |
N/A |
|
体积： |
0.24 mL |
|
保存： |
-25°C至-15°C |
|
纯度（SDS-PAGE） |
> 99％ |
|
活性： |
300,000 U/mg |
|
SS核酸外切酶 |
6,000 U< 1.0 % Released |
|
DS核酸外切酶 |
6,000 U< 1.0 % Released |
|
DS内切核酸酶 |
6,000 U=No Conversion |
|
大肠杆菌 DNA污染 |
6,000 U <10 copies |
蛋白质来源
携带克隆的T4 DNA连接酶基因的重组大肠杆菌。
提供：
10 mM Tris-HCl
50 mM KCl
1 mM dithiothreitol
0.1 mM EDTA
50% glycerol
pH 7.5 @ 25°C
随附：
B9020: 330mM Tris-HCl
50mM MgCl
5mM DTT
5mM ATP
30% PEG 6000
pH 7.6 @ 25°C
单位定义
1单位定义为在23℃孵育30分钟后，在50μl1X DNA连接酶缓冲液中加入100ng具有粘性末端的DNA的片段的50％所需的DNA连接酶的量。
分子量
55,292Da
质量控制分析
单位表征测定
单位活动使用2倍连续稀释法测量。在1X DNA连接酶反应缓冲液中进行酶批次的稀释并加入到20μL含有双链DNA的片段和1X DNA连接酶反应缓冲液的反应中。将反应在23℃孵育30分钟，停止，并在用溴化乙锭染色的1％琼脂糖凝胶上分析。
蛋白质浓度由OD 280吸光度确定。
物理纯度通过浓缩和稀释的酶溶液的SDS-PAGE评估，然后进行银染检测。通过比较浓缩样品中污染物条带的总质量与稀释样品中目的蛋白质条带的质量来评估纯度。
单链核酸外切酶在含有放射性标记的单链DNA底物的50μL反应液中和10μL酶溶液在37℃下温育4小时。
在含有放射性标记的双链DNA底物的50μl反应液中和在37℃下温育4小时的10μL酶溶液测定双链核酸外切酶。
双链核酸内切酶在含有0.5μg质粒DNA的50μL反应液和10μL酶溶液中在37℃下温育4小时。
大肠杆菌的16S rDNA污染用5μL评价 ř的变性，并筛选酶溶液在的TaqMan qPCR测定对于污染的存在eplicate样品大肠杆菌使用对应于所述的16S rRNA基因座寡核苷酸引物的基因组DNA。
部分产品信息：
|
货号 |
品名 |
规格 |
浓度 |
品牌 |
|---|---|---|---|---|
|
N2070-10-L |
ATP Solution |
50 µmol |
10 mM |
Enzymatics |
|
N2050-10-L |
10 mM dNTP Solution Mix |
100 µmol |
10 mM |
Enzymatics |
|
N2010L |
100 mM 75µmol dNTP Solution Set |
75 µmol |
100 mM |
Enzymatics |
|
N2010-A-L |
100 mM dATP Solution |
75 µmol |
100 mM |
Enzymatics |
|
N2050L |
100µmol dNTP Solution Mix |
100 µmol |
25 mM |
Enzymatics |
|
Y9180L |
10x Uracil Cleavage System |
750 Reactions |
N/A |
Enzymatics |
|
P7670 |
2x HiFi PCR Master Mix |
1 rxn |
2x |
Enzymatics |
|
Y9420L |
5x ER/A-Tailing Enzyme Mix |
24 reactions |
N/A |
Enzymatics |
|
Y9380L |
E. coli Pyrophosphatase |
50 U |
100 U/mL |
Enzymatics |
|
Y9030L |
E. coli Single-Stranded DNA Binding Protein |
1.5 mg |
2.75 mg/ml |
Enzymatics |
|
Y9140-HC-L |
End-Repair Mix (High Concentration) |
75 Reactions |
1 Rxn/µL |
Enzymatics |
|
Y9140-LC-L |
End-Repair Mix (Low Concentration) |
200 Reactions |
1 Rxn/µL |
Enzymatics |
|
Y9080L |
Endonuclease VIII |
10,000 U |
10,000 U/ml |
Enzymatics |
|
P7600L |
EnzScript™ (MMLV Reverse Transcriptase RNase H-) |
10,000 U |
200,000 U/mL |
Enzymatics |
|
X8010L |
Exonuclease I |
30,000 U |
20,000 U/ml |
Enzymatics |
|
X8020L |
Exonuclease III |
50,000 U |
100,000 U/ml |
Enzymatics |
|
P7010-HC-L |
Klenow (3′→ 5′ exo-) (High Concentration) |
10,000 U |
50,000 U/ml |
Enzymatics |
|
P7010-LC-L |
Klenow (3′→ 5′ exo-) (Low Concentration) |
10,000 U |
5,000 U/ml |
Enzymatics |
|
P7060L |
Klenow Fragment |
2,500 U |
5,000 U/ml |
Enzymatics |
|
X8030L |
Lambda Exonuclease |
10,000 U |
5,000 U/ml |
Enzymatics |
|
P7040L |
M-MuLV Reverse Transcriptase |
100,000 U |
200,000 U/ml |
Enzymatics |
|
P7090L |
Mako DNA Polymerase (3′→ 5′ exo-) |
3,000 U |
30,000 U/ml |
Enzymatics |
|
P7140-HC-L |
Manta 1.0 DNA Polymerase (High Concentration) |
100,000 U |
400,000 U/ml |
Enzymatics |
|
P7140-LC-L |
Manta 1.0 DNA Polymerase (Low Concentration) |
100,000 U |
40,000 U/ml |
Enzymatics |
|
P7610L |
NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions |
250 Reactions |
2X |
Enzymatics |
|
P7590L |
Phoenix™ Hot Start Taq DNA Polymerase |
500 U |
5,000 U/ml |
Enzymatics |
|
P7460L |
Poly(A) Polymerase |
1,000 U |
5,000 U/mL |
Enzymatics |
|
Y9260L |
RecA |
1.5 mg |
2.0 mg/mL |
Enzymatics |
|
Y9220L |
RNAse H |
5,000 U |
5,000 U/ml |
Enzymatics |
|
Y9240L |
RNAse Inhibitor |
20,000 U |
40,000 U/ml |
Enzymatics |
|
L6010L |
T3 DNA Ligase |
900,000 U |
3,000,000 U/ml |
Enzymatics |
|
L6030-LC-L |
T4 DNA Ligase |
150,000 U |
120,000 U/ml |
Enzymatics |
|
L6030-HC-L |
T4 DNA Ligase (Rapid) |
240,000 U |
600,000 U/ml |
Enzymatics |
|
P7080L |
T4 DNA Polymerase |
2,000 U |
3,000 U/ml |
Enzymatics |
|
Y9130L |
T4 Gene 32 Protein |
1.0 mg |
10 mg/mL |
Enzymatics |
|
Y9040L |
T4 Polynucleotide Kinase |
10,000 U |
10,000 U/ml |
Enzymatics |
|
L6050L |
T4 RNA Ligase 1 |
10,000 U |
20,000 U/ml |
Enzymatics |
|
L6080L |
T4 RNA Ligase 2 |
4,500 U |
30,000 U/ml |
Enzymatics |
|
L6070L |
T4 RNA Ligase 2 Truncated |
500 U |
5,000 U/ml |
Enzymatics |
|
L6020L |
T7 DNA Ligase |
900,000 U |
3,000,000 U/ml |
Enzymatics |
|
P7260L |
T7 DNA Polymerase |
3,500 U |
10,000 U/ml |
Enzymatics |
|
P7180L |
T7 RNA Polymerase |
50,000 U |
50,000 U/ml |
Enzymatics |
|
L6060L |
Taq DNA Ligase |
20,000 U |
40,000 U/ml |
Enzymatics |
|
P7110L |
Taq DSC 2.0 DNA Polymerase |
1,250 U |
5,000 U/ml |
Enzymatics |
|
P7250L |
Taq-B DNA Polymerase |
10,000 U |
5,000 U/ml |
Enzymatics |
|
P7620L |
TaqIT |
5,000 U |
50,000 U/mL |
Enzymatics |
|
P7070L |
Terminal deoxynucleotidyl Transferase (TdT) |
6,000 U |
20,000 U/ml |
Enzymatics |
|
G5020L |
Thermolabile UDG |
500 U |
1,000 U/ml |
Enzymatics |
|
Y9370L |
Thermostable Pyrophosphatase |
1,250 U |
2,000 U/ml |
Enzymatics |
|
G5010L |
Uracil DNA Glycosylase |
10,000 U |
2,000 U/ml |
Enzymatics |
|
P7511L |
VeraSeq™ 2.0 High-Fidelity DNA Polymerase |
500 U |
2,000 U/ml |
Enzymatics |
|
P7520L |
VeraSeq™ ULtra DNA Polymerase |
500 U |
2,000 U/ml |
Enzymatics |
|
L6030-W-L |
WGS Ligase |
24 Reactions |
N/A |
Enzymatics |
|
P7640L |
ZipScript™ One-Step RT-qPCR Kit |
1000 Reactions |
N/A |
Enzymatics |
|
P7020-HC-L |
φ29 DNA Polymerase (High Concentration) |
2,000 U |
100,000 U/ml |
Enzymatics |
|
P7020-LC-L |
φ29 DNA Polymerase (Low Concentration) |
2,000 U |
10,000 U/ml |
Enzymatics |
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