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Trans-Hi™ DNA Transfection Reagent (new)
!!! New And The Best !!!
We are very excited to announce the introduction of our brand new product, Trans-HiTM, as a superior transfection reagent to compete against the leading products in the market. Trans-HiTM is created based on a proprietary polymer technology. It forms stable complex particles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It offers superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-HiTM is very cost-efficient and 1.0mL is sufficient for over 650 transfections in 24 well plates.
Trans-Hi™ – In vitro Transfection Reagent
SKU:
F90101TH-10
Availability:
1day
* Select volume:
- 0.5 mL
- 1.0 mL
- 10 x 1.0 mL
Product Description
Trans-Hi™ is a superior transfection reagent capable to compete against the leading products on the market. It is based on a proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hi™ is very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates.
Key Features for Trans-HiTM
- Excellent transfection for single DNA transfection or multi DNA co-transfection
- Rapid onset and long-lasting transfection
- Suitable for very long DNAs
- Simple and easy to use transfection procedure
- Robust and reproducible transfection
- High level of recombinant protein production
- Very cost efficient
- Long shelf-life: 12 months
Specifications:
| Product type: | In vitro cell transfection reagent | |
| Applications | Plasmid transfection, protein overexpression | |
| Technique: | Polymer-based DNA nanoparticles | |
| Cell type: | Established cell lines, primary cells, hard-to-transfect cells Adherent cells and suspension cells | |
| Sample type: | Plasmid DNA | |
| Serum compatibility: | Yes | |
| Product size: | 0.5, 1.0 and 10×1.0 mL | |
| Number of transfections | Up to 660 in 24-well plates with 1.0mL reagent up to 330 transfections in 6-well plates | |
| Shelf-life: | 12 months or longer when store refrigerated (2-8°C) | |
| Shipping condition: | Room temperature |
Comparison study of In vitro plasmid DNA transfection of Trans-HiTM and Lipofectamine® 2000 in HEK293T cells with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and 0.5µg DNA per well used for both products in 24 well plate 30 hours post transfection.
Trans-Hi™/DNA ratio (µL/µg) (HEK293T)
Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in 24 well plate 30 hours post transfection, 0.5ug DNA per well).
Head-to-head comparison Trans-Hi™ against Lipofectamine 2000 HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).
| Trans-Hi™ | Lipofectamine-2000 | |||||
| Time | Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 |
| HEK293A (%) | 79.5 | 93.4 | 93.3 | 75.7 | 89.4 | 50.6 |
Comparison study of In vitro plasmid DNA transfection of Trans-Hi™ and Lipofectamine® 2000 in Hela cells with GFP vector ((reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).
Broad Transfection Spectrum for Mammalian Cell Types
|
Cell Lines |
Efficiency (% GFP) |
|
COS-7 |
85 – 90% |
|
Hela |
85 – 90% |
|
HEK-293T |
85-90% |
|
NIH 3T3 |
75% |
|
A549 |
75% |
|
Hep3D |
65-75% |
|
SHEP |
65-70% |
|
CaCo2 |
60% |
|
HepG2 |
70% |
|
SKOV3 |
65% |
|
MCF-7 |
65-70% |
|
3T3-442A |
35-40% |
|
CHO |
80-90% |